def main():
title=''
#1. Getting data
########################################################
########################################################
# df=pd.read_csv('int_data/seqs_rs_redef.csv') #Histone types info #Does not really seem that we need to redefine variants based on best score.
df=pd.read_csv('int_data/seqs_rs.csv') #Histone types info
fasta_dict=pickle.load( open( "int_data/fasta_dict.p", "rb" )) #Sequences
#2. Filtering - filter initial dataset by type, variant and other parameters
########################################################
########################################################
#2.1. Narrow by variant/type
########################################################
title+='H2A'
# f_df=df[(df['hist_var']=='canonical_H4')]
# f_df['hist_var']='canonical_H4'
f_df=df[((df['hist_var']=='canonical_H2A')|(df['hist_var']=='H2A.X'))&(df['partial']==False)&(df['non_st_aa']==False)]
# f_df=df[((df['hist_var']=='H2A.Z'))&(df['partial']==False)&(df['non_st_aa']==False)]
# f_df=df[(df['hist_type']=='H2A')]
print "Number of seqs after narrowing by hist type/var:", len(f_df)
#2.2. Filter by list of taxonomy clades - restrict sequences to certain taxonomic clades
#########################################################
title+=' across cellular organisms'
# parent_nodes=[9443] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
parent_nodes=[131567] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
#33682 - euglenozoa
#6656 - arthropods
# 4751 - fungi
#5782 - dictostelium
#This is akin manual removal of bad species
del_nodes=[5782,5690]
print "Selecting taxonomic subset for taxids: ",parent_nodes
print "while removing taxonomic subset for taxids: ",del_nodes
taxids=set(parent_nodes)
for i in parent_nodes:
taxids.update(ncbi.get_descendant_taxa(i,intermediate_nodes=True))
for i in del_nodes:
taxids=taxids.difference(set([i]))
taxids=taxids.difference(set(ncbi.get_descendant_taxa(i,intermediate_nodes=True)))
f_df=f_df[f_df['taxid'].isin(taxids)]
print "Number of seq after taxonomic subset: ",len(f_df)
#2.3.0 Marking number of identical sequence within each species and subspecies.
#This will simplify further analysis of sequence filtering on similarity
#We know that all refseqs are duplicated for instance.
################################################
ident=dict()
new_gis=list()
tids=set(list(f_df['taxid']))
for i in tids:
# print i.name, i.sci_name
temp_df=f_df[(f_df['taxid']==i)]
gis=list(temp_df['gi']) #this is to limit exec time
# print gis
if(len(gis)>1):
res=cluster_seq_support({gi:fasta_dict[str(gi)] for gi in gis},ident_thresh=1.00)
ident.update(res)
else:
ident.update({gis[0]:1})
f_df['ident']=[ident.get(k,1) for k in f_df['gi']]
#where ident - number of identical sequnces for current sepecies/subspecies.
print "Identity of sequence inside each taxid determined"
#2.3.1. Calculate number of similar seqs for every seq in tax group
#########################################################
# Use powerful method, to get rid of random errors is to identify identical sequences
# if a sequence is supported by two or more entires - this is good.
# Here we add a degen column to our data set - showing how many similar sequences are found
# for a given sequence in its taxonomic clade (genus currently)
#We will traverse the species tree by species, genus or family, and determine degeneracy level
degen=dict()
new_gis=list()
tids=list(f_df['taxid'])
t = ncbi.get_topology(tids,intermediate_nodes=True)
for i in t.search_nodes(rank='family'):
# print i.name, i.sci_name
nodeset=list()
for k in i.traverse():
nodeset.append(int(k.name))
temp_df=f_df[(f_df['taxid'].isin(nodeset))]
gis=list(temp_df['gi']) #this is to limit exec time
# print gis
res=cluster_seq_support({gi:fasta_dict[str(gi)] for gi in gis},ident_thresh=1.00)
degen.update(res)
# print degen
f_df['degen']=[degen.get(k,1) for k in f_df['gi']]
#2.3.2. Remove seqs that do not have support outside their species
# if they are not curated or RefSeq NP.
###########################################################
f_df=f_df.sort(['RefSeq','degen'],ascending=False) # so that RefSeq record get priority on removing duplicates
f_df=f_df[(f_df['degen']>f_df['ident'])|(f_df['curated']==True)|(f_df['RefSeq']==2)]
print "After removing mined seqs with no support in neighboring species: ",len(f_df)
#2.3.3. Shuffle sequnces, so that upon further selection, RefSeq and high degeneracy get priority
###########################################################
#RefSeq and degenerate sequence get priority
# title+=' 1ptax'
f_df=f_df.sort(['RefSeq','degen'],ascending=False) # so that RefSeq record get priority on removing duplicates
# print f_df[0:10]
# f_df=f_df.drop_duplicates(['taxid','hist_var'])
#2.4 Take one best representative per specific taxonomic rank (e.g. genus)
############################################################
pruningrank='genus'
print "Pruning taxonomy by ", pruningrank
title+=' , one seq. per %s'%pruningrank
#Common ranks: superorder-order-suborder-infraorder-parvorder-superfamily-family-subfamily-genus-species-subspecies
seqtaxids=list(f_df['taxid']) #old list
grouped_taxids=group_taxids(seqtaxids,rank=pruningrank)
# print seqtaxids
# print grouped_taxids
#Now we need to take best representative
#refseq NP, curated, or the one with largest degeneracy
new_gis=list()
for tids in grouped_taxids:
t_df=f_df[f_df['taxid'].isin(tids)]
#try take curated first
if(len(t_df[t_df['curated']==True])>0):
new_gis.append(t_df.loc[t_df.curated==True,'gi'].values[0])
continue
#try take NP records nest
#RefSeq 2 means NP, 1 means XP
if(len(t_df[t_df['RefSeq']==2])>0):
new_gis.append(t_df.loc[t_df.RefSeq==2,'gi'].values[0])
continue
# take best degenerate otherwise
else:
t_df=t_df.sort(['degen','RefSeq'],ascending=False)
new_gis.append(t_df['gi'].iloc[0])
f_df=f_df[f_df['gi'].isin(new_gis)]
print "After pruning taxonomy we have: ",len(f_df)
#2.5. Check seq for sanity - needs to be checked!
##############################################
# title+=' seqQC '
# print "Checkig sequence quality"
# newgis=list()
# for i,row in f_df.iterrows():
# gi=row['gi']
# seq=fasta_dict[str(gi)].seq
# hist_type=row['hist_type']
# hist_var=row['hist_var']
# if(check_hist_length(seq,hist_type,hist_var,5)&check_hist_core_length(seq,hist_type,5)):
# newgis.append(gi)
# f_df=f_df[f_df['gi'].isin(newgis)] #remake the dataframe
# print len(f_df)
#3. Make a list of seq with good ids and descriptions
##############################################
f_fasta_dict={key: value for (key,value) in fasta_dict.iteritems() if int(key) in list(f_df['gi'])}
print len(f_fasta_dict)
taxid2name = ncbi.get_taxid_translator(list(f_df['taxid']))
#Relabel sequences gi=> type and organism
f_fasta_dict={key: SeqRecord(id=key, description=f_df.loc[f_df.gi==int(key),'hist_var'].values[0]+' '+taxid2name[f_df.loc[f_df.gi==int(key),'taxid'].values[0]],seq=value.seq) for (key,value) in f_fasta_dict.iteritems() }
#with arbitrary index
# f_fasta_dict_rel={key: SeqRecord(id=str(index), description=f_hist_df.loc[f_hist_df.gi==key,'hist_var'].values[0]+' '+taxid2names[f_hist_df.loc[f_hist_df.gi==key,'taxid'].values[0]],seq=f_fasta_dict[key].seq) for (index,key) in enumerate(f_fasta_dict) }
# exit()
#4. Make MSA
#################
#Here we construct MSA
msa=muscle_aln(f_fasta_dict.values(),gapopen=float(-20))
AlignIO.write(msa, "int_data/example_msa.fasta", "fasta")
msa_annot=MultipleSeqAlignment([SeqRecord(Seq(''.join(get_hist_ss_in_aln_as_string(msa)).replace(' ','-')),id='annotation',name='')])
msa_annot.extend(msa)
AlignIO.write(msa_annot, "int_data/example_msa_annot.fasta", "fasta")
for i in range(len(msa)):
gi=msa[i].id
msa[i].description=f_fasta_dict[gi].description.replace('canonical','ca')
msa.sort(key=lambda x: x.description)
#5. Visualize MSA############
aln2html(msa,'example_h2a.html',features=get_hist_ss_in_aln_for_html(msa,'H2A',0),title="canonical H2A alignment",description=True,field1w=10,field2w=35)
#6. Trim alignment - this is optional
#6.1. Trim gaps
# title+=' gaptrim'
# msa_tr=trim_aln_gaps(msa,threshold=0.8)
#6.2. Trim to histone core sequence
msa_tr=trim_hist_aln_to_core(msa)
# msa_tr=msa
# print get_hist_ss_in_aln_for_shade(msa_tr,below=True)
# exit()
#7. Vizualize MSA with ete2.##########
taxid2gi={f_df.loc[f_df.gi==int(gi),'taxid'].values[0]:gi for gi in list(f_df['gi'])}
gi2variant={gi:f_df.loc[f_df.gi==int(gi),'hist_var'].values[0] for gi in list(f_df['gi'])}
msa_dict={i.id:i.seq for i in msa_tr}
t = ncbi.get_topology(list(f_df['taxid']),intermediate_nodes=False)
a=t.add_child(name='annotation')
a.add_feature('sci_name','annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if(node.rank in ['order','class','phylum','kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name=='annotation':
s=str(msa_dict[str(taxid2gi[int(node.name)])])
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
gi=taxid2gi[int(node.name)]
add_face_to_node(TextFace(' '+str(gi)+' '),node,column=1, position = "aligned")
add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
if node.is_leaf() and node.name=='annotation':
s=get_hist_ss_in_aln_as_string(msa_tr)
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
add_face_to_node(TextFace(' '+'NCBI_GI'+' '),node,column=1, position = "aligned")
add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render("example_motifs_H2A.svg", w=6000, dpi=300, tree_style=ts)
#10. Conservation############
#############################
features=get_hist_ss_in_aln_for_shade(msa_tr,below=True)
cn=add_consensus(msa_tr,threshold=0.5)[-2:-1]
# Below are three methods that we find useful.
# plot_prof4seq('cons_sofp_psic',map(float,cons_prof(msa_tr,f=2,c=2)),cn,features,axis='conservation')
plot_prof4seq('example_cons_ent_unw',map(lambda x:log(20)+x,map(float,cons_prof(msa_tr,f=0,c=0))),cn,features,axis='conservation')
plot_prof4seq('example_cons_ent_unw_norm',map(lambda x:log(20)+x,map(float,cons_prof(msa_tr,f=0,c=0,norm="T"))),cn,features,axis='conservation')
# plot_prof4seq('cons_sofp_unw',map(float,cons_prof(msa_tr,f=0,c=2)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_unw_renorm1',map(float,cons_prof(msa_tr,f=0,c=2,m=1)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_unw',map(float,cons_prof(msa_tr,f=0,c=2,m=0)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_psic_renorm1',map(float,cons_prof(msa_tr,f=2,c=2,m=1)),cn,features,axis='conservation')
def main():
title=''
#1. Getting data
df=pd.read_csv('int_data/seqs_rs_redef.csv') #Histone types info
fasta_dict=pickle.load( open( "int_data/fasta_dict.p", "rb" )) #Sequences
# exit()
#2. Filtering
##########
#2.1. Narrow by variant/type
title+='Canonical H2A'
# f_df=df[(df['hist_var']=='canonical_H4')]
# f_df['hist_var']='canonical_H4'
f_df=df[(df['hist_var']=='canonical_H2A')|(df['hist_var']=='H2A.1')]
# f_df=df[(df['hist_type']=='H2A')]
# exit()
print len(f_df)
#2.2. #####select one variant per taxid
# title+=' 1ptax'
f_df=f_df.sort(['RefSeq'],ascending=False) # so that RefSeq record get priority on removing duplicates
f_df=f_df.drop_duplicates(['taxid','hist_var'])
# exit()
#2.3. Filter by list of taxonomy clades
################
title+=' across cellular organisms'
# parent_nodes=[9443] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
parent_nodes=[131567] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
#33682 - euglenozoa
#6656 - arthropods
# 4751 - fungi
print "Selecting taxonomic subset"
taxids=list(parent_nodes)
for i in parent_nodes:
taxids.extend(ncbi.get_descendant_taxa(i,intermediate_nodes=True))
f_df=f_df[f_df['taxid'].isin(taxids)]
print len(f_df)
# exit()
#2.4 Take one representative per specific taxonomic rank.
################
title+=', one sequence per order'
print "Pruning taxonomy"
#Common ranks: superorder-order-suborder-infraorder-parvorder-superfamily-family-subfamily-genus-species-subspecies
seqtaxids=list(f_df['taxid']) #old list
new_seqtaxids=subsample_taxids(seqtaxids,rank='order') #new subsampled list
f_df=f_df[f_df['taxid'].isin(new_seqtaxids)] #remake the dataframe
# print "---"
print len(f_df)
# exit()
#2.5. Check seq for sanity
################
# title+=' seqQC '
print "Checkig sequence quality"
newgis=list()
for i,row in f_df.iterrows():
gi=row['gi']
seq=fasta_dict[str(gi)].seq
hist_type=row['hist_type']
hist_var=row['hist_var']
if(check_hist_length(seq,hist_type,hist_var,1)&check_hist_core_length(seq,hist_type,1)):
newgis.append(gi)
f_df=f_df[f_df['gi'].isin(newgis)] #remake the dataframe
print len(f_df)
# print list(f_df['gi'])
# exit()
#3. Make a list of seq with good ids and descriptions
####################
f_fasta_dict={key: value for (key,value) in fasta_dict.iteritems() if int(key) in list(f_df['gi'])}
print len(f_fasta_dict)
taxid2name = ncbi.get_taxid_translator(list(f_df['taxid']))
#Relabel sequences gi=> type and organism
f_fasta_dict={key: SeqRecord(id=key, description=f_df.loc[f_df.gi==int(key),'hist_var'].values[0]+' '+taxid2name[f_df.loc[f_df.gi==int(key),'taxid'].values[0]],seq=value.seq) for (key,value) in f_fasta_dict.iteritems() }
#with arbitrary index
# f_fasta_dict_rel={key: SeqRecord(id=str(index), description=f_hist_df.loc[f_hist_df.gi==key,'hist_var'].values[0]+' '+taxid2names[f_hist_df.loc[f_hist_df.gi==key,'taxid'].values[0]],seq=f_fasta_dict[key].seq) for (index,key) in enumerate(f_fasta_dict) }
# exit()
#4. Make MSA
#################
#Here we construct MSA
msa=muscle_aln(f_fasta_dict.values())
AlignIO.write(msa, "results/h2a_ca_cellular.fasta", "fasta")
msa_annot=MultipleSeqAlignment([SeqRecord(Seq(''.join(get_hist_ss_in_aln_as_string(msa)).replace(' ','-')),id='annotation',name='')])
msa_annot.extend(msa)
AlignIO.write(msa_annot, "results/h2a_ca_cellular_annot.fasta", "fasta")
for i in range(len(msa)):
gi=msa[i].id
msa[i].description=f_fasta_dict[gi].description.replace('canonical','ca')
msa.sort(key=lambda x: x.description)
#5. Visualize MSA
aln2html(msa,'results/h2a_ca_cellular.html',features=get_hist_ss_in_aln_for_html(msa,'H2A',0),title="canonical H2A in cellular organisms",description=True,field1w=10,field2w=35)
#6. Trim alignment - this is optional
#6.1. Trim gaps
title+=', gaps removed'
# msa_tr=trim_aln_gaps(msa,threshold=0.8)
#6.2. Trim to histone core sequence
msa_tr=trim_hist_aln_to_core(msa)
#7. Vizualize MSA with ete2.
taxid2gi={f_df.loc[f_df.gi==int(gi),'taxid'].values[0]:gi for gi in list(f_df['gi'])}
gi2variant={gi:f_df.loc[f_df.gi==int(gi),'hist_var'].values[0] for gi in list(f_df['gi'])}
msa_dict={i.id:i.seq for i in msa_tr}
print taxid2gi
t = ncbi.get_topology(list(f_df['taxid']),intermediate_nodes=False)
a=t.add_child(name='annotation')
a.add_feature('sci_name','annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if(node.rank in ['order','class','phylum','kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name=='annotation':
s=str(msa_dict[str(taxid2gi[int(node.name)])])
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
gi=taxid2gi[int(node.name)]
add_face_to_node(TextFace(' '+str(gi)+' '),node,column=1, position = "aligned")
add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
if node.is_leaf() and node.name=='annotation':
s=get_hist_ss_in_aln_as_string(msa_tr)
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
add_face_to_node(TextFace(' '+'NCBI_GI'+' '),node,column=1, position = "aligned")
add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render("results/h2a_ca_cellular.svg", w=6000, dpi=300, tree_style=ts)
#10. Conservation
features=get_hist_ss_in_aln_for_shade(msa_tr,below=True)
cn=add_consensus(msa_tr,threshold=0.5)[-2:-1]
# Below are three methods that we find useful.
# plot_prof4seq('cons_sofp_psic',map(float,cons_prof(msa_tr,f=2,c=2)),cn,features,axis='conservation')
plot_prof4seq('results/h2a_ca_cellular_cons_ent_unw',map(lambda x:log(20)+x,map(float,cons_prof(msa_tr,f=0,c=0))),cn,features,axis='conservation',title='Conservation, canonical H2A cellular organisms')
# plot_prof4seq('cons_sofp_unw',map(float,cons_prof(msa_tr,f=0,c=2)),cn,features,axis='conservation')
plot_prof4seq('results/h2a_ca_cellular_cons_sofp_unw_renorm1',map(float,cons_prof(msa_tr,f=0,c=2,m=1)),cn,features,axis='conservation',title='Conservation, canonical H2A cellular organisms')
plot_prof4seq('results/h2a_ca_cellular_cons_sofp_psic_renorm1',map(float,cons_prof(msa_tr,f=2,c=2,m=1)),cn,features,axis='conservation',title='Conservation, canonical H2A cellular organisms')
def main():
title=''
#1. Getting data
df=pd.read_csv('int_data/seqs_rs_redef.csv') #Histone types info
fasta_dict=pickle.load( open( "int_data/fasta_dict.p", "rb" )) #Sequences
# exit()
#2. Filtering
##########
#2.1. Narrow by variant/type
title+='CenH3'
# f_df=df[(df['hist_var']=='canonical_H4')]
# f_df['hist_var']='canonical_H4'
f_df=df[((df['hist_var']=='cenH3'))&(df['partial']==False)]
# f_df=df[(df['hist_type']=='H2A')]
# exit()
print len(f_df)
#2.2. Filter by list of taxonomy clades
################
title+=' across cellular organisms'
# parent_nodes=[9443] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
parent_nodes=[131567] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
#33682 - euglenozoa
#6656 - arthropods
# 4751 - fungi
#5782 - dictostelium
print "Selecting taxonomic subset"
taxids=list(parent_nodes)
for i in parent_nodes:
taxids.extend(ncbi.get_descendant_taxa(i,intermediate_nodes=True))
f_df=f_df[f_df['taxid'].isin(taxids)]
print len(f_df)
# exit()
#2.3*. Alternative powerful method, to get rid of random errors in seqs
#We need to cluster seqs, and select only if we have support by two or more similar seqs
#We will traverse the species tree by species, genus or family, and determine degeneracy level
degen=dict()
new_gis=list()
tids=list(f_df['taxid'])
t = ncbi.get_topology(tids,intermediate_nodes=True)
for i in t.search_nodes(rank='genus'):
# print i.name, i.sci_name
nodeset=list()
for k in i.traverse():
nodeset.append(int(k.name))
temp_df=f_df[(f_df['taxid'].isin(nodeset))]
gis=list(temp_df['gi']) #this is to limit exec time
# print gis
res=cluster_seq_support({gi:fasta_dict[str(gi)] for gi in gis},ident_thresh=0.95)
degen.update(res)
# exit()
# for k,v in res.iteritems():
# if v>2.0:
# new_gis.append(k)
# f_df=f_df[f_df['gi'].isin(new_gis)]
print degen
f_df['degen']=[degen.get(k,1) for k in f_df['gi']]
#2.4. #####select one variant per taxid, priority to RefSeq
# title+=' 1ptax'
f_df=f_df.sort(['RefSeq','degen'],ascending=False) # so that RefSeq record get priority on removing duplicates
print f_df[0:10]
# f_df=f_df.drop_duplicates(['taxid','hist_var'])
#2.4 Take one best representative per specific taxonomic rank.
################
title+=' , one seq. per genus, trimmed'
print "Pruning taxonomy"
#Common ranks: superorder-order-suborder-infraorder-parvorder-superfamily-family-subfamily-genus-species-subspecies
seqtaxids=list(f_df['taxid']) #old list
grouped_taxids=group_taxids(seqtaxids,rank='species')
print seqtaxids
print grouped_taxids
#Now we need to take best representative
#refseq NP, or the one with larges degeneracy
new_gis=list()
for tids in grouped_taxids:
t_df=f_df[f_df['taxid'].isin(tids)]
#take NP if we have it
if(len(t_df[t_df['RefSeq']==2])>0):
new_gis.append(t_df.loc[t_df.RefSeq==2,'gi'].values[0])
continue
else: # take best degenerate
t_df=t_df.sort(['degen','RefSeq'],ascending=False) # so that RefSeq record get priority on removing duplicates
if(t_df['degen'].iloc[0]>100):
new_gis.append(t_df['gi'].iloc[0])
f_df=f_df[f_df['gi'].isin(new_gis)]
# new_seqtaxids=subsample_taxids(seqtaxids,rank='species') #new subsampled list
# f_df=f_df[f_df['taxid'].isin(new_seqtaxids)] #remake the dataframe
# print "---"
# exit()
#2.5. Check seq for sanity
################
# title+=' seqQC '
# print "Checkig sequence quality"
# newgis=list()
# for i,row in f_df.iterrows():
# gi=row['gi']
# seq=fasta_dict[str(gi)].seq
# hist_type=row['hist_type']
# hist_var=row['hist_var']
# if(check_hist_length(seq,hist_type,hist_var,5)&check_hist_core_length(seq,hist_type,5)):
# newgis.append(gi)
# f_df=f_df[f_df['gi'].isin(newgis)] #remake the dataframe
# print len(f_df)
#3. Make a list of seq with good ids and descriptions
####################
f_fasta_dict={key: value for (key,value) in fasta_dict.iteritems() if int(key) in list(f_df['gi'])}
print len(f_fasta_dict)
taxid2name = ncbi.get_taxid_translator(list(f_df['taxid']))
#Relabel sequences gi=> type and organism
f_fasta_dict={key: SeqRecord(id=key, description=f_df.loc[f_df.gi==int(key),'hist_var'].values[0]+' '+taxid2name[f_df.loc[f_df.gi==int(key),'taxid'].values[0]],seq=value.seq) for (key,value) in f_fasta_dict.iteritems() }
#with arbitrary index
# f_fasta_dict_rel={key: SeqRecord(id=str(index), description=f_hist_df.loc[f_hist_df.gi==key,'hist_var'].values[0]+' '+taxid2names[f_hist_df.loc[f_hist_df.gi==key,'taxid'].values[0]],seq=f_fasta_dict[key].seq) for (index,key) in enumerate(f_fasta_dict) }
# exit()
#4. Make MSA
#################
#Here we construct MSA
msa=muscle_aln(f_fasta_dict.values(),gapopen=float(-20))
AlignIO.write(msa, "int_data/example_msa.fasta", "fasta")
msa_annot=MultipleSeqAlignment([SeqRecord(Seq(''.join(get_hist_ss_in_aln_as_string(msa)).replace(' ','-')),id='annotation',name='')])
msa_annot.extend(msa)
AlignIO.write(msa_annot, "int_data/example_msa_annot.fasta", "fasta")
for i in range(len(msa)):
gi=msa[i].id
msa[i].description=f_fasta_dict[gi].description.replace('canonical','ca')
msa.sort(key=lambda x: x.description)
#5. Visualize MSA
aln2html(msa,'example_h2a.html',features=get_hist_ss_in_aln_for_html(msa,'H2A',0),title="canonical H2A alignment",description=True,field1w=10,field2w=35)
#6. Trim alignment - this is optional
#6.1. Trim gaps
# title+=' gaptrim'
# msa_tr=trim_aln_gaps(msa,threshold=0.8)
#6.2. Trim to histone core sequence
msa_tr=trim_hist_aln_to_core(msa)
# msa_tr=msa
# print get_hist_ss_in_aln_for_shade(msa_tr,below=True)
# exit()
#7. Vizualize MSA with ete2.
taxid2gi={f_df.loc[f_df.gi==int(gi),'taxid'].values[0]:gi for gi in list(f_df['gi'])}
gi2variant={gi:f_df.loc[f_df.gi==int(gi),'hist_var'].values[0] for gi in list(f_df['gi'])}
msa_dict={i.id:i.seq for i in msa_tr}
print taxid2gi
t = ncbi.get_topology(list(f_df['taxid']),intermediate_nodes=False)
a=t.add_child(name='annotation')
a.add_feature('sci_name','annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if(node.rank in ['order','class','phylum','kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name=='annotation':
s=str(msa_dict[str(taxid2gi[int(node.name)])])
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
gi=taxid2gi[int(node.name)]
add_face_to_node(TextFace(' '+str(gi)+' '),node,column=1, position = "aligned")
add_face_to_node(TextFace(' '+str(int(node.name))+' '),node,column=2, position = "aligned")
add_face_to_node(TextFace(' '+str(gi2variant[gi])+' '),node,column=3, position = "aligned")
if node.is_leaf() and node.name=='annotation':
s=get_hist_ss_in_aln_as_string(msa_tr)
seqFace = SeqMotifFace(s,[[0,len(s), "seq", 10, 10, None, None, None]],scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
add_face_to_node(TextFace(' '+'NCBI_GI'+' '),node,column=1, position = "aligned")
add_face_to_node(TextFace(' '+'NCBI_TAXID'+' '),node,column=2, position = "aligned")
add_face_to_node(TextFace(' '+'Variant'+' '),node,column=3, position = "aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render("example_motifs_H2A.svg", w=6000, dpi=300, tree_style=ts)
#10. Conservation
features=get_hist_ss_in_aln_for_shade(msa_tr,below=True)
cn=add_consensus(msa_tr,threshold=0.5)[-2:-1]
# Below are three methods that we find useful.
# plot_prof4seq('cons_sofp_psic',map(float,cons_prof(msa_tr,f=2,c=2)),cn,features,axis='conservation')
plot_prof4seq('example_cons_ent_unw',map(lambda x:log(20)+x,map(float,cons_prof(msa_tr,f=0,c=0))),cn,features,axis='conservation')
# plot_prof4seq('cons_sofp_unw',map(float,cons_prof(msa_tr,f=0,c=2)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_unw_renorm1',map(float,cons_prof(msa_tr,f=0,c=2,m=1)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_unw',map(float,cons_prof(msa_tr,f=0,c=2,m=0)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_psic_renorm1',map(float,cons_prof(msa_tr,f=2,c=2,m=1)),cn,features,axis='conservation')
def main():
title = ''
#1. Getting data
########################################################
########################################################
# df=pd.read_csv('int_data/seqs_rs_redef.csv') #Histone types info #Does not really seem that we need to redefine variants based on best score.
df = pd.read_csv('int_data/seqs_rs.csv') #Histone types info
fasta_dict = pickle.load(open("int_data/fasta_dict.p", "rb")) #Sequences
#2. Filtering - filter initial dataset by type, variant and other parameters
########################################################
########################################################
#2.1. Narrow by variant/type
########################################################
title += 'H2A'
# f_df=df[(df['hist_var']=='canonical_H4')]
# f_df['hist_var']='canonical_H4'
f_df = df[(
(df['hist_var'] == 'canonical_H2A') | (df['hist_var'] == 'H2A.X'))
& (df['partial'] == False) & (df['non_st_aa'] == False)]
# f_df=df[((df['hist_var']=='H2A.Z'))&(df['partial']==False)&(df['non_st_aa']==False)]
# f_df=df[(df['hist_type']=='H2A')]
print "Number of seqs after narrowing by hist type/var:", len(f_df)
#2.2. Filter by list of taxonomy clades - restrict sequences to certain taxonomic clades
#########################################################
title += ' across cellular organisms'
# parent_nodes=[9443] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
parent_nodes = [
131567
] #131567 - cellular organisms, 7215 4930 Drosophila and yeast, 9443 - primates
#33682 - euglenozoa
#6656 - arthropods
# 4751 - fungi
#5782 - dictostelium
#This is akin manual removal of bad species
del_nodes = [5782, 5690]
print "Selecting taxonomic subset for taxids: ", parent_nodes
print "while removing taxonomic subset for taxids: ", del_nodes
taxids = set(parent_nodes)
for i in parent_nodes:
taxids.update(ncbi.get_descendant_taxa(i, intermediate_nodes=True))
for i in del_nodes:
taxids = taxids.difference(set([i]))
taxids = taxids.difference(
set(ncbi.get_descendant_taxa(i, intermediate_nodes=True)))
f_df = f_df[f_df['taxid'].isin(taxids)]
print "Number of seq after taxonomic subset: ", len(f_df)
#2.3.0 Marking number of identical sequence within each species and subspecies.
#This will simplify further analysis of sequence filtering on similarity
#We know that all refseqs are duplicated for instance.
################################################
ident = dict()
new_gis = list()
tids = set(list(f_df['taxid']))
for i in tids:
# print i.name, i.sci_name
temp_df = f_df[(f_df['taxid'] == i)]
gis = list(temp_df['gi']) #this is to limit exec time
# print gis
if (len(gis) > 1):
res = cluster_seq_support({gi: fasta_dict[str(gi)]
for gi in gis},
ident_thresh=1.00)
ident.update(res)
else:
ident.update({gis[0]: 1})
f_df['ident'] = [ident.get(k, 1) for k in f_df['gi']]
#where ident - number of identical sequnces for current sepecies/subspecies.
print "Identity of sequence inside each taxid determined"
#2.3.1. Calculate number of similar seqs for every seq in tax group
#########################################################
# Use powerful method, to get rid of random errors is to identify identical sequences
# if a sequence is supported by two or more entires - this is good.
# Here we add a degen column to our data set - showing how many similar sequences are found
# for a given sequence in its taxonomic clade (genus currently)
#We will traverse the species tree by species, genus or family, and determine degeneracy level
degen = dict()
new_gis = list()
tids = list(f_df['taxid'])
t = ncbi.get_topology(tids, intermediate_nodes=True)
for i in t.search_nodes(rank='family'):
# print i.name, i.sci_name
nodeset = list()
for k in i.traverse():
nodeset.append(int(k.name))
temp_df = f_df[(f_df['taxid'].isin(nodeset))]
gis = list(temp_df['gi']) #this is to limit exec time
# print gis
res = cluster_seq_support({gi: fasta_dict[str(gi)]
for gi in gis},
ident_thresh=1.00)
degen.update(res)
# print degen
f_df['degen'] = [degen.get(k, 1) for k in f_df['gi']]
#2.3.2. Remove seqs that do not have support outside their species
# if they are not curated or RefSeq NP.
###########################################################
f_df = f_df.sort(
['RefSeq', 'degen'], ascending=False
) # so that RefSeq record get priority on removing duplicates
f_df = f_df[(f_df['degen'] > f_df['ident']) | (f_df['curated'] == True) |
(f_df['RefSeq'] == 2)]
print "After removing mined seqs with no support in neighboring species: ", len(
f_df)
#2.3.3. Shuffle sequnces, so that upon further selection, RefSeq and high degeneracy get priority
###########################################################
#RefSeq and degenerate sequence get priority
# title+=' 1ptax'
f_df = f_df.sort(
['RefSeq', 'degen'], ascending=False
) # so that RefSeq record get priority on removing duplicates
# print f_df[0:10]
# f_df=f_df.drop_duplicates(['taxid','hist_var'])
#2.4 Take one best representative per specific taxonomic rank (e.g. genus)
############################################################
pruningrank = 'genus'
print "Pruning taxonomy by ", pruningrank
title += ' , one seq. per %s' % pruningrank
#Common ranks: superorder-order-suborder-infraorder-parvorder-superfamily-family-subfamily-genus-species-subspecies
seqtaxids = list(f_df['taxid']) #old list
grouped_taxids = group_taxids(seqtaxids, rank=pruningrank)
# print seqtaxids
# print grouped_taxids
#Now we need to take best representative
#refseq NP, curated, or the one with largest degeneracy
new_gis = list()
for tids in grouped_taxids:
t_df = f_df[f_df['taxid'].isin(tids)]
#try take curated first
if (len(t_df[t_df['curated'] == True]) > 0):
new_gis.append(t_df.loc[t_df.curated == True, 'gi'].values[0])
continue
#try take NP records nest
#RefSeq 2 means NP, 1 means XP
if (len(t_df[t_df['RefSeq'] == 2]) > 0):
new_gis.append(t_df.loc[t_df.RefSeq == 2, 'gi'].values[0])
continue
# take best degenerate otherwise
else:
t_df = t_df.sort(['degen', 'RefSeq'], ascending=False)
new_gis.append(t_df['gi'].iloc[0])
f_df = f_df[f_df['gi'].isin(new_gis)]
print "After pruning taxonomy we have: ", len(f_df)
#2.5. Check seq for sanity - needs to be checked!
##############################################
# title+=' seqQC '
# print "Checkig sequence quality"
# newgis=list()
# for i,row in f_df.iterrows():
# gi=row['gi']
# seq=fasta_dict[str(gi)].seq
# hist_type=row['hist_type']
# hist_var=row['hist_var']
# if(check_hist_length(seq,hist_type,hist_var,5)&check_hist_core_length(seq,hist_type,5)):
# newgis.append(gi)
# f_df=f_df[f_df['gi'].isin(newgis)] #remake the dataframe
# print len(f_df)
#3. Make a list of seq with good ids and descriptions
##############################################
f_fasta_dict = {
key: value
for (key, value) in fasta_dict.iteritems()
if int(key) in list(f_df['gi'])
}
print len(f_fasta_dict)
taxid2name = ncbi.get_taxid_translator(list(f_df['taxid']))
#Relabel sequences gi=> type and organism
f_fasta_dict = {
key: SeqRecord(
id=key,
description=f_df.loc[f_df.gi == int(key), 'hist_var'].values[0] +
' ' + taxid2name[f_df.loc[f_df.gi == int(key), 'taxid'].values[0]],
seq=value.seq)
for (key, value) in f_fasta_dict.iteritems()
}
#with arbitrary index
# f_fasta_dict_rel={key: SeqRecord(id=str(index), description=f_hist_df.loc[f_hist_df.gi==key,'hist_var'].values[0]+' '+taxid2names[f_hist_df.loc[f_hist_df.gi==key,'taxid'].values[0]],seq=f_fasta_dict[key].seq) for (index,key) in enumerate(f_fasta_dict) }
# exit()
#4. Make MSA
#################
#Here we construct MSA
msa = muscle_aln(f_fasta_dict.values(), gapopen=float(-20))
AlignIO.write(msa, "int_data/example_msa.fasta", "fasta")
msa_annot = MultipleSeqAlignment([
SeqRecord(Seq(''.join(get_hist_ss_in_aln_as_string(msa)).replace(
' ', '-')),
id='annotation',
name='')
])
msa_annot.extend(msa)
AlignIO.write(msa_annot, "int_data/example_msa_annot.fasta", "fasta")
for i in range(len(msa)):
gi = msa[i].id
msa[i].description = f_fasta_dict[gi].description.replace(
'canonical', 'ca')
msa.sort(key=lambda x: x.description)
#5. Visualize MSA############
aln2html(msa,
'example_h2a.html',
features=get_hist_ss_in_aln_for_html(msa, 'H2A', 0),
title="canonical H2A alignment",
description=True,
field1w=10,
field2w=35)
#6. Trim alignment - this is optional
#6.1. Trim gaps
# title+=' gaptrim'
# msa_tr=trim_aln_gaps(msa,threshold=0.8)
#6.2. Trim to histone core sequence
msa_tr = trim_hist_aln_to_core(msa)
# msa_tr=msa
# print get_hist_ss_in_aln_for_shade(msa_tr,below=True)
# exit()
#7. Vizualize MSA with ete2.##########
taxid2gi = {
f_df.loc[f_df.gi == int(gi), 'taxid'].values[0]: gi
for gi in list(f_df['gi'])
}
gi2variant = {
gi: f_df.loc[f_df.gi == int(gi), 'hist_var'].values[0]
for gi in list(f_df['gi'])
}
msa_dict = {i.id: i.seq for i in msa_tr}
t = ncbi.get_topology(list(f_df['taxid']), intermediate_nodes=False)
a = t.add_child(name='annotation')
a.add_feature('sci_name', 'annotation')
t.sort_descendants(attr='sci_name')
ts = TreeStyle()
def layout(node):
# print node.rank
# print node.sci_name
if getattr(node, "rank", None):
if (node.rank in ['order', 'class', 'phylum', 'kingdom']):
rank_face = AttrFace("sci_name", fsize=7, fgcolor="indianred")
node.add_face(rank_face, column=0, position="branch-top")
if node.is_leaf():
sciname_face = AttrFace("sci_name", fsize=9, fgcolor="steelblue")
node.add_face(sciname_face, column=0, position="branch-right")
if node.is_leaf() and not node.name == 'annotation':
s = str(msa_dict[str(taxid2gi[int(node.name)])])
seqFace = SeqMotifFace(
s, [[0, len(s), "seq", 10, 10, None, None, None]],
scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
gi = taxid2gi[int(node.name)]
add_face_to_node(TextFace(' ' + str(gi) + ' '),
node,
column=1,
position="aligned")
add_face_to_node(TextFace(' ' + str(int(node.name)) + ' '),
node,
column=2,
position="aligned")
add_face_to_node(TextFace(' ' + str(gi2variant[gi]) + ' '),
node,
column=3,
position="aligned")
if node.is_leaf() and node.name == 'annotation':
s = get_hist_ss_in_aln_as_string(msa_tr)
seqFace = SeqMotifFace(
s, [[0, len(s), "seq", 10, 10, None, None, None]],
scale_factor=1)
add_face_to_node(seqFace, node, 0, position="aligned")
add_face_to_node(TextFace(' ' + 'NCBI_GI' + ' '),
node,
column=1,
position="aligned")
add_face_to_node(TextFace(' ' + 'NCBI_TAXID' + ' '),
node,
column=2,
position="aligned")
add_face_to_node(TextFace(' ' + 'Variant' + ' '),
node,
column=3,
position="aligned")
ts.layout_fn = layout
ts.show_leaf_name = False
ts.title.add_face(TextFace(title, fsize=20), column=0)
t.render("example_motifs_H2A.svg", w=6000, dpi=300, tree_style=ts)
#10. Conservation############
#############################
features = get_hist_ss_in_aln_for_shade(msa_tr, below=True)
cn = add_consensus(msa_tr, threshold=0.5)[-2:-1]
# Below are three methods that we find useful.
# plot_prof4seq('cons_sofp_psic',map(float,cons_prof(msa_tr,f=2,c=2)),cn,features,axis='conservation')
plot_prof4seq('example_cons_ent_unw',
map(lambda x: log(20) + x,
map(float, cons_prof(msa_tr, f=0, c=0))),
cn,
features,
axis='conservation')
plot_prof4seq('example_cons_ent_unw_norm',
map(lambda x: log(20) + x,
map(float, cons_prof(msa_tr, f=0, c=0, norm="T"))),
cn,
features,
axis='conservation')
# plot_prof4seq('cons_sofp_unw',map(float,cons_prof(msa_tr,f=0,c=2)),cn,features,axis='conservation')
plot_prof4seq('example_cons_sofp_unw_renorm1',
map(float, cons_prof(msa_tr, f=0, c=2, m=1)),
cn,
features,
axis='conservation')
plot_prof4seq('example_cons_sofp_unw',
map(float, cons_prof(msa_tr, f=0, c=2, m=0)),
cn,
features,
axis='conservation')
plot_prof4seq('example_cons_sofp_psic_renorm1',
map(float, cons_prof(msa_tr, f=2, c=2, m=1)),
cn,
features,
axis='conservation')