def execute(self,ops):
reagents=None
for iteration in range(2):
print "Iteration ",iteration+1
# Need to clear here
trp=TRP()
if iteration==0:
trp.addTemplates(["IN"],200) # Add a template with stock concentration 200nM
else:
reagents=Sample.getAllOnPlate(Experiment.REAGENTPLATE)
for r in reagents:
if r.volume<0:
r.initvolume=-r.volume+10
Sample.clearall()
ops(self)
trp.finish()
from Experiment.sample import Sample
from Experiment.experiment import Experiment
import math
from TRPLib.trp import TRP
reagents = None
input = "BT409"
srcprefix = "B"
prodprefix = "A"
for iteration in range(2):
print "Iteration ", iteration + 1
trp = TRP()
if iteration == 0:
trp.addTemplates([input],
200) # Add a template with stock concentration 200nM
else:
reagents = Sample.getAllOnPlate(Experiment.REAGENTPLATE)
for r in reagents:
if r.volume < 0:
r.initvolume = -r.volume + 20
Sample.clearall()
# Round 1 (Keep uncleaved +theo)
t71 = trp.runT7(theo=[False, True],
src=[input, input],
tgt=[],
vol=[16, 18],
srcdil=4)
sv1t7 = trp.saveSamps(src=t71, tgt=[], vol=10, dil=[4, 4])
rt1 = trp.runRT(pos=[True, True], src=t71, tgt=[], vol=[15, 22], srcdil=2)