def run(self, config, config_file, parallel, dirs, samples):
## Alignment and preparation requiring the entire input file (multicore cluster)
with prun.start(_wres(parallel, ["aligner", "samtools", "sambamba"],
(["reference", "fasta"], ["reference", "aligner"], ["files"])),
samples, config, dirs, "multicore",
multiplier=alignprep.parallel_multiplier(samples)) as run_parallel:
with profile.report("alignment preparation", dirs):
samples = run_parallel("prep_align_inputs", samples)
samples = disambiguate.split(samples)
with profile.report("alignment", dirs):
samples = run_parallel("process_alignment", samples)
samples = alignprep.merge_split_alignments(samples, run_parallel)
samples = disambiguate.resolve(samples, run_parallel)
with profile.report("callable regions", dirs):
samples = run_parallel("postprocess_alignment", samples)
samples = run_parallel("combine_sample_regions", [samples])
samples = region.clean_sample_data(samples)
with profile.report("coverage", dirs):
samples = coverage.summarize_samples(samples, run_parallel)
## Variant calling on sub-regions of the input file (full cluster)
with prun.start(_wres(parallel, ["gatk", "picard", "variantcaller"]),
samples, config, dirs, "full",
multiplier=region.get_max_counts(samples), max_multicore=1) as run_parallel:
with profile.report("alignment post-processing", dirs):
samples = region.parallel_prep_region(samples, run_parallel)
with profile.report("variant calling", dirs):
samples = genotype.parallel_variantcall_region(samples, run_parallel)
## Finalize variants (per-sample cluster)
with prun.start(_wres(parallel, ["gatk", "gatk-vqsr", "snpeff", "bcbio_variation"]),
samples, config, dirs, "persample") as run_parallel:
with profile.report("joint squaring off/backfilling", dirs):
samples = joint.square_off(samples, run_parallel)
with profile.report("variant post-processing", dirs):
samples = run_parallel("postprocess_variants", samples)
samples = run_parallel("split_variants_by_sample", samples)
with profile.report("validation", dirs):
samples = run_parallel("compare_to_rm", samples)
samples = genotype.combine_multiple_callers(samples)
## Finalizing BAMs and population databases, handle multicore computation
with prun.start(_wres(parallel, ["gemini", "samtools", "fastqc", "bamtools", "bcbio_variation",
"bcbio-variation-recall"]),
samples, config, dirs, "multicore2") as run_parallel:
with profile.report("prepped BAM merging", dirs):
samples = region.delayed_bamprep_merge(samples, run_parallel)
with profile.report("ensemble calling", dirs):
samples = ensemble.combine_calls_parallel(samples, run_parallel)
with profile.report("validation summary", dirs):
samples = validate.summarize_grading(samples)
with profile.report("structural variation", dirs):
samples = structural.run(samples, run_parallel)
with profile.report("population database", dirs):
samples = population.prep_db_parallel(samples, run_parallel)
with profile.report("quality control", dirs):
samples = qcsummary.generate_parallel(samples, run_parallel)
with profile.report("archive", dirs):
samples = archive.compress(samples, run_parallel)
logger.info("Timing: finished")
return samples
def run(self, config, config_file, parallel, dirs, samples):
## Alignment and preparation requiring the entire input file (multicore cluster)
with prun.start(_wres(parallel, ["aligner", "samtools", "sambamba"],
(["reference", "fasta"], ["reference", "aligner"], ["files"])),
samples, config, dirs, "multicore",
multiplier=alignprep.parallel_multiplier(samples)) as run_parallel:
with profile.report("alignment preparation", dirs):
samples = run_parallel("prep_align_inputs", samples)
samples = disambiguate.split(samples)
with profile.report("alignment", dirs):
samples = run_parallel("process_alignment", samples)
samples = alignprep.merge_split_alignments(samples, run_parallel)
samples = disambiguate.resolve(samples, run_parallel)
with profile.report("callable regions", dirs):
samples = run_parallel("postprocess_alignment", samples)
samples = run_parallel("combine_sample_regions", [samples])
samples = region.clean_sample_data(samples)
with profile.report("coverage", dirs):
samples = coverage.summarize_samples(samples, run_parallel)
## Variant calling on sub-regions of the input file (full cluster)
with prun.start(_wres(parallel, ["gatk", "picard", "variantcaller"]),
samples, config, dirs, "full",
multiplier=region.get_max_counts(samples), max_multicore=1) as run_parallel:
with profile.report("alignment post-processing", dirs):
samples = region.parallel_prep_region(samples, run_parallel)
with profile.report("variant calling", dirs):
samples = genotype.parallel_variantcall_region(samples, run_parallel)
## Finalize variants (per-sample cluster)
with prun.start(_wres(parallel, ["gatk", "gatk-vqsr", "snpeff", "bcbio_variation"]),
samples, config, dirs, "persample") as run_parallel:
with profile.report("variant post-processing", dirs):
samples = run_parallel("postprocess_variants", samples)
samples = run_parallel("split_variants_by_sample", samples)
with profile.report("validation", dirs):
samples = run_parallel("compare_to_rm", samples)
samples = genotype.combine_multiple_callers(samples)
## Finalizing BAMs and population databases, handle multicore computation
with prun.start(_wres(parallel, ["gemini", "samtools", "fastqc", "bamtools", "bcbio_variation",
"bcbio-variation-recall"]),
samples, config, dirs, "multicore2") as run_parallel:
with profile.report("prepped BAM merging", dirs):
samples = region.delayed_bamprep_merge(samples, run_parallel)
with profile.report("ensemble calling", dirs):
samples = ensemble.combine_calls_parallel(samples, run_parallel)
with profile.report("validation summary", dirs):
samples = validate.summarize_grading(samples)
with profile.report("structural variation", dirs):
samples = structural.run(samples, run_parallel)
with profile.report("population database", dirs):
samples = population.prep_db_parallel(samples, run_parallel)
with profile.report("quality control", dirs):
samples = qcsummary.generate_parallel(samples, run_parallel)
with profile.report("archive", dirs):
samples = archive.compress(samples, run_parallel)
logger.info("Timing: finished")
return samples
def run(self, config, config_file, run_parallel, parallel, dirs, samples):
## Alignment and preparation requiring the entire input file (multicore cluster)
with global_parallel(parallel, "multicore", ["process_alignment", "postprocess_alignment"],
samples, dirs, config,
multiplier=alignprep.parallel_multiplier(samples)) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: alignment")
samples = run_parallel("prep_align_inputs", samples)
samples = disambiguate.split(samples)
samples = run_parallel("process_alignment", samples)
samples = alignprep.merge_split_alignments(samples, run_parallel)
samples = disambiguate.resolve(samples, run_parallel)
samples = run_parallel("postprocess_alignment", samples)
regions = callable.combine_sample_regions(samples)
samples = region.add_region_info(samples, regions)
samples = region.clean_sample_data(samples)
logger.info("Timing: coverage")
samples = coverage.summarize_samples(samples, run_parallel)
## Variant calling on sub-regions of the input file (full cluster)
with global_parallel(parallel, "full", ["piped_bamprep", "variantcall_sample"],
samples, dirs, config,
multiplier=len(regions["analysis"]), max_multicore=1) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: alignment post-processing")
samples = region.parallel_prep_region(samples, regions, run_parallel)
logger.info("Timing: variant calling")
samples = region.parallel_variantcall_region(samples, run_parallel)
## Finalize variants (per-sample cluster)
with global_parallel(parallel, "persample", ["postprocess_variants"],
samples, dirs, config) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: variant post-processing")
samples = run_parallel("postprocess_variants", samples)
logger.info("Timing: validation")
samples = run_parallel("compare_to_rm", samples)
samples = combine_multiple_callers(samples)
logger.info("Timing: ensemble calling")
samples = ensemble.combine_calls_parallel(samples, run_parallel)
samples = validate.summarize_grading(samples)
## Finalizing BAMs and population databases, handle multicore computation
with global_parallel(parallel, "multicore2", ["prep_gemini_db", "delayed_bam_merge"],
samples, dirs, config) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: prepped BAM merging")
samples = region.delayed_bamprep_merge(samples, run_parallel)
logger.info("Timing: structural variation")
samples = structural.run(samples, run_parallel)
logger.info("Timing: population database")
samples = population.prep_db_parallel(samples, run_parallel)
logger.info("Timing: quality control")
samples = qcsummary.generate_parallel(samples, run_parallel)
logger.info("Timing: finished")
return samples
def run(self, config, config_file, run_parallel, parallel, dirs,
lane_items):
## Alignment and preparation requiring the entire input file (multicore cluster)
with global_parallel(parallel, "multicore", ["align_prep_full"],
lane_items, dirs, config) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: alignment")
samples = run_parallel(
"align_prep_full",
[list(x) + [config_file] for x in lane_items])
regions = callable.combine_sample_regions(samples)
samples = region.add_region_info(samples, regions)
samples = region.clean_sample_data(samples)
logger.info("Timing: coverage")
samples = coverage.summarize_samples(samples, run_parallel)
## Variant calling on sub-regions of the input file (full cluster)
with global_parallel(parallel,
"full", ["piped_bamprep", "variantcall_sample"],
samples,
dirs,
config,
multiplier=len(regions["analysis"])) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: alignment post-processing")
samples = region.parallel_prep_region(samples, regions,
run_parallel)
logger.info("Timing: variant calling")
samples = region.parallel_variantcall_region(samples, run_parallel)
## Finalize variants (per-sample cluster)
with global_parallel(parallel, "persample", ["postprocess_variants"],
samples, dirs, config) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: variant post-processing")
samples = run_parallel("postprocess_variants", samples)
logger.info("Timing: validation")
samples = run_parallel("compare_to_rm", samples)
samples = combine_multiple_callers(samples)
logger.info("Timing: ensemble calling")
samples = ensemble.combine_calls_parallel(samples, run_parallel)
samples = validate.summarize_grading(samples)
logger.info("Timing: quality control")
samples = qcsummary.generate_parallel(samples, run_parallel)
## Finalizing BAMs and population databases, handle multicore computation
with global_parallel(parallel, "multicore2",
["prep_gemini_db", "delayed_bam_merge"], samples,
dirs, config) as parallel:
run_parallel = parallel_runner(parallel, dirs, config)
logger.info("Timing: prepped BAM merging")
samples = region.delayed_bamprep_merge(samples, run_parallel)
logger.info("Timing: population database")
samples = population.prep_db_parallel(samples, run_parallel)
logger.info("Timing: finished")
return samples
def run(self, config, run_info_yaml, parallel, dirs, samples):
## Alignment and preparation requiring the entire input file (multicore cluster)
with prun.start(_wres(parallel, ["aligner", "samtools", "sambamba"],
(["reference", "fasta"], ["reference", "aligner"], ["files"])),
samples, config, dirs, "multicore",
multiplier=alignprep.parallel_multiplier(samples)) as run_parallel:
with profile.report("organize samples", dirs):
samples = run_parallel("organize_samples", [[dirs, config, run_info_yaml,
[x[0]["description"] for x in samples]]])
with profile.report("alignment preparation", dirs):
samples = run_parallel("prep_align_inputs", samples)
samples = run_parallel("disambiguate_split", [samples])
with profile.report("alignment", dirs):
samples = run_parallel("process_alignment", samples)
samples = disambiguate.resolve(samples, run_parallel)
samples = alignprep.merge_split_alignments(samples, run_parallel)
with profile.report("callable regions", dirs):
samples = run_parallel("prep_samples", [samples])
samples = run_parallel("postprocess_alignment", samples)
samples = run_parallel("combine_sample_regions", [samples])
samples = region.clean_sample_data(samples)
with profile.report("coverage", dirs):
samples = coverage.summarize_samples(samples, run_parallel)
with profile.report("structural variation initial", dirs):
samples = structural.run(samples, run_parallel, initial_only=True)
## Variant calling on sub-regions of the input file (full cluster)
with prun.start(_wres(parallel, ["gatk", "picard", "variantcaller"]),
samples, config, dirs, "full",
multiplier=region.get_max_counts(samples), max_multicore=1) as run_parallel:
with profile.report("alignment post-processing", dirs):
samples = region.parallel_prep_region(samples, run_parallel)
with profile.report("variant calling", dirs):
samples = genotype.parallel_variantcall_region(samples, run_parallel)
## Finalize variants, BAMs and population databases (per-sample multicore cluster)
with prun.start(_wres(parallel, ["gatk", "gatk-vqsr", "snpeff", "bcbio_variation",
"gemini", "samtools", "fastqc", "bamtools",
"bcbio-variation-recall", "qsignature"]),
samples, config, dirs, "multicore2") as run_parallel:
with profile.report("joint squaring off/backfilling", dirs):
samples = joint.square_off(samples, run_parallel)
with profile.report("variant post-processing", dirs):
samples = run_parallel("postprocess_variants", samples)
samples = run_parallel("split_variants_by_sample", samples)
with profile.report("prepped BAM merging", dirs):
samples = region.delayed_bamprep_merge(samples, run_parallel)
with profile.report("validation", dirs):
samples = run_parallel("compare_to_rm", samples)
samples = genotype.combine_multiple_callers(samples)
with profile.report("ensemble calling", dirs):
samples = ensemble.combine_calls_parallel(samples, run_parallel)
with profile.report("validation summary", dirs):
samples = validate.summarize_grading(samples)
with profile.report("structural variation final", dirs):
samples = structural.run(samples, run_parallel)
with profile.report("heterogeneity", dirs):
samples = heterogeneity.run(samples, run_parallel)
with profile.report("population database", dirs):
samples = population.prep_db_parallel(samples, run_parallel)
with profile.report("quality control", dirs):
samples = qcsummary.generate_parallel(samples, run_parallel)
with prun.start(_wres(parallel, ["gatk"], ensure_mem = {"gatk": 8}),
samples, config, dirs, "coverage") as run_parallel:
with profile.report("report", dirs):
samples = qcsummary.report_summary(samples, run_parallel)
with profile.report("archive", dirs):
samples = archive.compress(samples, run_parallel)
with profile.report("upload", dirs):
samples = run_parallel("upload_samples", samples)
for sample in samples:
run_parallel("upload_samples_project", [sample])
logger.info("Timing: finished")
return samples