def read_channels(img_path, series):
dapi = bioformats.load_image(img_path, c=0, series=series, rescale=False)
mt = bioformats.load_image(img_path, c=1, series=series, rescale=False)
rsv1 = bioformats.load_image(img_path, c=2, series=series, rescale=False)
tub = bioformats.load_image(img_path, c=3, series=series, rescale=False)
return dapi, mt, rsv1, tub
def plot_overlay_from_czi_timepoints(path_file, timepoint_one, timepoint_two):
"""
Plot two timepoints from a czi image in a red/green overlay. Warning: requires a running javabridge
:param path_file: Path to the czi file
:param timepoint_one: First timepoint
:param timepoint_two: Second timepoint
:return:
"""
array_one = bf.load_image(str(path_file), t=timepoint_one)
array_two = bf.load_image(str(path_file), t=timepoint_two)
image_one = sitk.GetImageFromArray(array_one)
image_two = sitk.GetImageFromArray(array_two)
itkplot.plot_overlay(image_one, image_two, sitk.Transform(2, sitk.sitkIdentity), continuous_update=True,
downsample=False)
class ImageReader(object):
def ReadImages(self, fds):
'''fds -- list of file descriptors (filenames or urls)
returns a list of channels as numpy float32 arrays
'''
return self.read_images_via_bioformats(fds)
def _read_image_via_bioformats(self, filename_or_url):
# The opener's destructor deletes the temprary files, so the
# opener must not be GC'ed until the image has been loaded.
opener = ThrowingURLopener()
if p.image_url_prepend:
parsed = urlparse.urlparse(p.image_url_prepend + filename_or_url)
if parsed.scheme:
try:
filename_or_url, ignored_headers = opener.retrieve(
parsed.geturl())
except IOError, e:
if e.args[0] == 'http error':
status_code, message = e.args[1:3]
raise ClearException(
'Failed to load image from %s' % parsed.geturl(),
'%d %s' % (status_code, message))
else:
raise
logging.info('Loading image from "%s"' % filename_or_url)
return bioformats.load_image(filename_or_url)
def main(args):
if hasattr(sys, 'frozen'):
jar_path = os.path.join(os.path.dirname(sys.executable), "jars")
jars = [os.path.join(jar_path, os.path.split(jar)[1])
for jar in bioformats.JARS]
else:
jars = bioformats.JARS
javabridge.start_vm(class_path=jars)
try:
filenames = sum(map(glob.glob, sys.argv[1:]), [])
app = wx.PySimpleApp()
if len(filenames) == 0:
with wx.FileDialog(
None,
"Pick files for z-stack",
wildcard="Tiff files (*.tif)|*.tif|All files (*.*)|*.*",
style = wx.FD_FILE_MUST_EXIST | wx.FD_MULTIPLE | wx.FD_OPEN) as dlg:
assert isinstance(dlg, wx.FileDialog)
if dlg.ShowModal() != wx.ID_OK:
return
filenames = dlg.Paths
planes = [bioformats.load_image(filename) for filename in filenames]
img_red, img_green, img_blue = [
np.dstack([plane[:, :, i] for plane in planes]) *255
for i in range(3)]
frame = Q3DFrame(img_red, img_green, img_blue, None,
size=(1024, 768))
frame.SetTitle("Q3DStack: %s" %filenames[0])
app.MainLoop()
finally:
javabridge.kill_vm()
def load_image(image_path,
c=None,
z=0,
t=0,
series=None,
index=None,
rescale=True,
wants_max_intensity=False,
channel_names=None):
"""
Load in image from a file using bioformats, always returning a tuple of
(image, scale) where the image is a numpy.ndarray object, and the scale
is an integer or None.
"""
result = bioformats.load_image(path=image_path,
c=c,
z=z,
t=t,
series=series,
index=index,
rescale=rescale,
wants_max_intensity=wants_max_intensity,
channel_names=channel_names)
if wants_max_intensity:
# The value of result is a tuple: ( image, scale )
image, scale = result
else:
image = result
scale = None
# The image is a numpy.ndarray object, and the scale is an integer or None.
return image, scale
def run(self, workspace):
image_name = self.image_name.value
measurements = workspace.measurements
image_set = workspace.image_set
M_FILE_NAME = cpmeas.C_FILE_NAME + "_" + image_name
M_PATH_NAME = cpmeas.C_PATH_NAME + "_" + image_name
#
# Use measurements.get_measurement to get the file name and path name
# for this cycle
#
path_name = measurements.get_measurement(cpmeas.IMAGE, M_PATH_NAME)
file_name = measurements.get_measurement(cpmeas.IMAGE, M_FILE_NAME)
#
# use os.path.join(pathname, filename) to get the name for BioFormats
#
path = os.path.join(path_name, file_name)
#
# call load_image(path) to load the file into a numpy array
#
pixel_data = load_image(path)
#
# make a cpi.Image for the image data
#
image = cpi.Image(pixel_data, path_name = path_name, file_name = file_name)
#
# call image_set.add to add the image to the image set
#
image_set.add(image_name, image)
def ProcessFileList(self):
self.Nfiles = len(self.video_list)
for idx in range(self.Nfiles):
input_video = self.video_list[idx]
input_roif = self.roif_list[idx]
input_ext = os.path.splitext(input_video)[1]
self.output_prefix = os.path.join(self.save_direct,
os.path.splitext(
os.path.basename(input_video))[0])
#File that contains the ROI coordinates
this_roif = np.array(bioformats.load_image(input_roif,rescale=False))
self.roi_buff_dict,self.roi_path_dict = self.growROI(this_roif,self.roi_buffer)
#Output file for intensity timeseries
self.this_ofile = os.path.join(
self.save_direct,
os.path.basename(
input_video
).replace(input_ext,'.csv')
)
self.this_nofile = self.this_ofile.replace('.csv','_normalized.csv')
self.this_noplot = self.this_nofile.replace('.csv','.pdf')
#Currently tested importing nd2 files or TIFF files...theoretically can load any bioformats file
if input_ext=='.nd2':
self.ProcessND2(input_video)
else:
self.ProcessOther(input_video)
def test_load_using_bioformats():
path = os.path.join(os.path.dirname(__file__), 'resources',
'Channel1-01-A-01.tif')
image, scale = bioformats.load_image(path,
rescale=False,
wants_max_intensity=True)
print(image.shape)
def measure_image(image_name):
sample = re.sub('_pc_fov_[0-9]*_[0-9]*.czi', '', image_name)
image = bioformats.load_image(image_name, rescale=False)
confocal_image_name = '{}_fov_1_488.czi'.format(sample)
confocal_image = bioformats.load_image(confocal_image_name, rescale=False)
segmentation = segment_images(image)
cells = skimage.measure.regionprops(segmentation)
average_photon_count = np.empty((len(cells), image.shape[2]))
total_photon_count = np.empty((len(cells), image.shape[2]))
for k in range(0, image.shape[2]):
cells = skimage.measure.regionprops(segmentation,
intensity_image=image[:, :, k])
average_photon_count[:, k] = [x.mean_intensity for x in cells]
total_photon_count[:, k] = [x.mean_intensity * x.area for x in cells]
average_confocal_intensity = np.empty((len(cells), image.shape[2]))
total_confocal_intensity = np.empty((len(cells), image.shape[2]))
for k in range(0, image.shape[2]):
cells = skimage.measure.regionprops(
segmentation, intensity_image=confocal_image[:, :, k])
average_confocal_intensity[:, k] = [x.mean_intensity for x in cells]
total_confocal_intensity[:, k] = [
x.mean_intensity * x.area for x in cells
]
cell_areas = [x.area * 0.07 * 0.07 for x in cells]
fig = plt.figure()
fig.set_size_inches(cm_to_inches(8), cm_to_inches(5))
plt.hist(cell_areas, bins=100, color=(0, 0.5, 1), histtype='step')
plt.xlabel(r'$A_c$ [$\mu m^2$]', fontsize=8, color=theme_color)
plt.ylabel('Frequency', fontsize=8, color=theme_color)
plt.tick_params(direction='in', length=2, labelsize=8, colors=theme_color)
plt.axes().spines['left'].set_color(theme_color)
plt.axes().spines['bottom'].set_color(theme_color)
plt.axes().spines['right'].set_color(theme_color)
plt.axes().spines['top'].set_color(theme_color)
plt.subplots_adjust(left=0.22, bottom=0.25, right=0.98, top=0.97)
plt.savefig('{}_cell_area.pdf'.format(sample), dpi=300, transparent=True)
plt.close()
save_segmentation(segmentation, sample)
average_photon_counts_filename = '{}_average_photon_counts.csv'.format(
sample)
total_photon_counts_filename = '{}_total_photon_counts.csv'.format(sample)
pd.DataFrame(average_photon_count).to_csv(average_photon_counts_filename,
index=None)
pd.DataFrame(total_photon_count).to_csv(total_photon_counts_filename,
index=None)
return
def max_projection(path, t_slice, z_slices, image_channel):
count = 0
for z in z_slices:
if z == np.min(z_slices):
test_img = bioformats.load_image(path=path,
t=t_slice,
z=z,
series=0)
shape = np.shape(test_img)
img_array = np.zeros([shape[0], shape[1], len(z_slices)])
img_loop = bioformats.load_image(path=path, t=t_slice, z=z,
series=0) * 65535
if len(np.shape(img_loop)) > 2:
img_loop = img_loop[:, :, image_channel]
img_array[:, :, count] = img_loop
count += 1
max_intensity = np.amax(img_array, axis=2)
return max_intensity
def read_test_image(file_name, **kwargs):
'''Read an image from the test directory
file_name - name of the file within the test directory
**kwargs - arguments passe into load_image
'''
from bioformats import load_image
path = os.path.join(testimages_directory, file_name)
return load_image(path, **kwargs)
def read_test_image(file_name, **kwargs):
'''Read an image from the test directory
file_name - name of the file within the test directory
**kwargs - arguments passe into load_image
'''
from bioformats import load_image
path = os.path.join(testimages_directory, file_name)
return load_image(path, **kwargs)
def load_tif_stack(fname, limit=None):
stack = []
for i in itertools.count():
if not limit or i < limit:
try:
stack.append(bioformats.load_image(fname, t=i))
except javabridge.jutil.JavaException:
break
else:
break
return np.array(stack)
def load_tif_stack(fname, limit=None):
stack = []
for i in itertools.count():
if not limit or i < limit:
try:
stack.append(bioformats.load_image(fname, t=i))
except javabridge.jutil.JavaException:
break
else:
break
return np.array(stack)
def read_example_image(folder, file_name, **kwargs):
'''Read an example image from one of the example image directories
folder - folder containing images, e.g. "ExampleFlyImages"
file_name - the name of the file within the folder
**kwargs - any keyword arguments are passed onto load_image
'''
from bioformats import load_image
path = os.path.join(example_images_directory(), folder, file_name)
return load_image(path, **kwargs)
def provide_image(self, image_set):
"""Load an image from a pathname
"""
if self.__image is not None:
return self.__image
if self.volume:
return self.get_image_volume()
self.cache_file()
filename = self.get_filename()
channel_names = []
url = self.get_url()
properties = {}
if self.index is None:
metadata = bioformats.get_omexml_metadata(self.get_full_name())
ometadata = bioformats.omexml.OMEXML(metadata)
pixel_metadata = ometadata.image(
0 if self.series is None else self.series).Pixels
nplanes = pixel_metadata.SizeC * pixel_metadata.SizeZ * pixel_metadata.SizeT
indexes = list(range(nplanes))
elif numpy.isscalar(self.index):
indexes = [self.index]
else:
indexes = self.index
planes = []
offset = 0
for i, index in enumerate(indexes):
properties["index"] = str(index)
if self.series is not None:
if numpy.isscalar(self.series):
properties["series"] = self.series
else:
properties["series"] = self.series[i]
img = bioformats.load_image(self.get_full_name(),
rescale=False,
**properties).astype(int)
img = convert_image_to_objects(img).astype(numpy.int32)
img[img != 0] += offset
offset += numpy.max(img)
planes.append(img)
image = Image(
numpy.dstack(planes),
path_name=self.get_pathname(),
file_name=self.get_filename(),
convert=False,
)
self.__image = image
return image
def read_example_image(folder, file_name, **kwargs):
'''Read an example image from one of the example image directories
folder - folder containing images, e.g. "ExampleFlyImages"
file_name - the name of the file within the folder
**kwargs - any keyword arguments are passed onto load_image
'''
from bioformats import load_image
path = os.path.join(example_images_directory(), folder, file_name)
maybe_download_example_image([folder], file_name)
return load_image(path, **kwargs)
def czi_timepoint_to_sitk_image(path_file, position, resolution, resolution_unit='microns'):
"""
Open a timepoint from a czi image and make it into an ITK image. Warning: requires a running javabridge.
:param path_file: Path to the czi file
:param position: Timepoint to open
:param resolution: Resolution of the czi image
:param resolution_unit: The unit of measure (e.g. microns) of the czi image
:return: A SimpleITK image made from the timepoint
"""
array = bf.load_image(str(path_file), t=position)
image = sitk.GetImageFromArray(array)
image.SetSpacing([resolution, resolution])
image.SetMetaData('Unit', resolution_unit)
return image
def _read_image(self, filename_or_url, log_io=True):
# The opener's destructor deletes the temprary files, so the
# opener must not be GC'ed until the image has been loaded.
opener = ThrowingURLopener()
if p.image_url_prepend:
parsed = urllib.parse.urlparse(p.image_url_prepend +
filename_or_url)
if parsed.scheme:
try:
filename_or_url, ignored_headers = opener.retrieve(
parsed.geturl())
except IOError as e:
if e.args[0] == 'http error':
status_code, message = e.args[1:3]
raise ClearException(
'Failed to load image from %s' % parsed.geturl(),
'%d %s' % (status_code, message))
else:
raise
if not p.force_bioformats and os.path.splitext(
filename_or_url)[-1].lower() in IMAGEIO_FORMATS:
if log_io:
logging.info('ImageIO: Loading image from "%s"' %
filename_or_url)
try:
return imageio.imread(filename_or_url)
except FileNotFoundError:
logging.error(f"File not found: {filename_or_url}")
return
except:
logging.info(
'Loading with ImageIO failed, falling back to BioFormats')
if javabridge.get_env() is None:
logging.debug("Starting javabridge")
javabridge.start_vm(class_path=bioformats.JARS, run_headless=True)
javabridge.attach()
if log_io:
logging.info('BioFormats: Loading image from "%s"' %
filename_or_url)
try:
return bioformats.load_image(filename_or_url, rescale=False)
except FileNotFoundError:
logging.error(f"File not found: {filename_or_url}")
except:
logging.error(
'Loading with BioFormats failed, file may be corrupted or unavailable'
)
def load_TIFF(path, opath, serie=0):
meta, _ = _metadata(path, serie=serie)
image = np.empty((meta['SizeX'], meta['SizeY'], meta['SizeC']))
#with bioformats.ImageReader(path) as rdr:
# for t in range(0, meta['SizeT']):
# for z in range(0, meta['SizeZ']):
# for c in range(0, meta['SizeC']):
# image[:,:,c]=rdr.read(c=c, z=z, t=t, series=serie,
# index=None, rescale=False, wants_max_intensity=False,
# channel_names=None)
img = bioformats.load_image(path, c=0)
directory = _new_directory(opath, meta)
return (img, directory, meta)
def load_slide_scanner_image(self, z=0, serie=4):
self.downfactor = 1
self._img_vsi = bf.load_image(self.path.as_posix(),
t=0,
series=serie,
z=z)
metadata = bf.get_omexml_metadata(self.path.as_posix())
o = bf.OMEXML(metadata)
n_zslices = o.image_count
self.n_slices_known.emit(n_zslices, self._img_vsi.shape[-1], 4, 1)
self.slice_slide_scanner()
def read_bf(path):
"""
read an image into a np-array using BioFormats
Parameters
----------
path: str
file path to read
Returns
-------
img: np.array
image as np-array
"""
javabridge.start_vm(class_path=bioformats.JARS, run_headless=True)
img = bioformats.load_image(path, rescale=False)
return img
def _read_image_via_bioformats(self, filename_or_url):
# The opener's destructor deletes the temprary files, so the
# opener must not be GC'ed until the image has been loaded.
opener = ThrowingURLopener()
if p.image_url_prepend:
parsed = urlparse.urlparse(p.image_url_prepend + filename_or_url)
if parsed.scheme:
try:
filename_or_url, ignored_headers = opener.retrieve(parsed.geturl())
except IOError as e:
if e.args[0] == 'http error':
status_code, message = e.args[1:3]
raise ClearException(
'Failed to load image from %s' % parsed.geturl(),
'%d %s' % (status_code, message))
else:
raise
logging.info('Loading image from "%s"' % filename_or_url)
return bioformats.load_image(filename_or_url)
def _read_image_via_bioformats(self, filename_or_url):
# The opener's destructor deletes the temprary files, so the
# opener must not be GC'ed until the image has been loaded.
opener = ThrowingURLopener()
if p.image_url_prepend:
parsed = urlparse.urlparse(p.image_url_prepend + filename_or_url)
if parsed.scheme:
try:
filename_or_url, ignored_headers = opener.retrieve(
parsed.geturl())
except IOError as e:
if e.args[0] == 'http error':
status_code, message = e.args[1:3]
raise ClearException(
'Failed to load image from %s' % parsed.geturl(),
'%d %s' % (status_code, message))
else:
raise
logging.info('Loading image from "%s"' % filename_or_url)
return bioformats.load_image(filename_or_url)
def measure_reference_images(image_name, cal_toggle, calibration_norm):
sample = re.sub('_[0-9]*.czi', '', image_name[0])
print('Analyzing sample {}...'.format(sample))
image_stack = [bioformats.load_image(filename) for filename in image_name]
segmentation, image_stack = segment_images(image_stack)
if cal_toggle == 'T':
image_ffc = correct_images(image_stack, calibration_norm)
else:
image_ffc = image_stack.copy()
cells = skimage.measure.regionprops(segmentation)
avgint = np.empty((len(cells), image_ffc.shape[2]))
for k in range(0, image_ffc.shape[2]):
cells = skimage.measure.regionprops(segmentation,
intensity_image=image_ffc[:, :, k])
avgint[:, k] = [x.mean_intensity for x in cells]
save_segmentation(segmentation, sample)
avgint_norm = avgint / np.max(avgint, axis=1)[:, None]
avgintfilename = sample + '_avgint.csv'
avgintnormfilename = sample + '_avgint_norm.csv'
np.savetxt(avgintfilename, avgint, delimiter=',')
np.savetxt(avgintnormfilename, avgint_norm, delimiter=',')
return (segmentation, avgint)
def correctBackground(self,input_frame,input_video,channel,is_nd2=False):
if self.ts == 0:
for idx in range(self.irrad_frame):
if is_nd2:
this_frame = input_video.get_frame_2D(c=channel,t=idx)
else:
this_frame = np.array(bioformats.load_image(input_video,c=self.protein_channel,t=self.ts,rescale=False))
self.histogram,bins = np.histogram(this_frame,
bins=np.arange(1,np.max(this_frame)+1))
#Smooth the histogram out
temp = np.convolve(self.histogram,
np.ones(3,dtype=int),'valid')
ranges = np.arange(1,2,2)
start = np.cumsum(self.histogram[:2])[::2]/ranges
stop = (np.cumsum(self.histogram[:-3:-1])[::2]/ranges)[::-1]
self.smoothed_hist = np.concatenate((start,temp,stop))
self.peaks = scipy.signal.find_peaks(self.smoothed_hist)[0]
self.min_peak_int = np.max(self.smoothed_hist[self.peaks])
self.min_peak = np.where(self.smoothed_hist==self.min_peak_int)[0][0]
self.bg_value = bins[np.where(
self.smoothed_hist[self.min_peak:]<=\
(0.67*self.min_peak_int))[0][0]]\
+self.min_peak
self.avg_bg = np.average(this_frame[np.where(
input_frame<=self.bg_value)])
try:
self.arr_of_avgs = np.append(self.arr_of_avgs,
self.avg_bg)
except:
self.arr_of_avgs = np.array([self.avg_bg])
self.bg_correction = np.average(self.arr_of_avgs)
output_frame = input_frame - self.bg_correction
output_frame[np.where(output_frame < 0)] = 0
return output_frame
def load_image( image_path, c=None, z=0, t=0, series=None, index=None,
rescale=True, wants_max_intensity=False, channel_names=None ):
"""
Load in image from a file using bioformats, always returning a tuple of
(image, scale) where the image is a numpy.ndarray object, and the scale
is an integer or None.
"""
result = bioformats.load_image( path=image_path,
c=c,
z=z,
t=t,
series=series,
index=index,
rescale=rescale,
wants_max_intensity=wants_max_intensity,
channel_names=channel_names )
if wants_max_intensity:
# The value of result is a tuple: ( image, scale )
image, scale = result
else:
image = result
scale = None
# The image is a numpy.ndarray object, and the scale is an integer or None.
return image, scale
import sys
matplotlib.use('WXAgg')
from wx.lib.inspection import InspectionTool
import matplotlib.pyplot
from scipy.ndimage import label
from centrosome.otsu import otsu
javabridge.start_vm(class_path=bioformats.JARS)
try:
app = wx.PySimpleApp()
figure = matplotlib.figure.Figure()
images = []
objects = []
masks = []
for i, arg in enumerate(sys.argv[1:]):
img = bioformats.load_image(arg)
images.append(
ImageData("Image %d" % (i+1), img,
alpha = 1.0 / (len(sys.argv) - 1),
mode = MODE_COLORIZE))
thresh = otsu(img)
l, _ = label(img >= thresh, np.ones((3,3), bool))
outline_color = tuple([int(idx == i) for idx in range(3)])
objects.append(ObjectsData(
"Objects %d" % (i+1), [l],
outline_color = outline_color,
mode = MODE_LINES))
ii = np.linspace(-1, 1, num = img.shape[0])[:, np.newaxis]
jj = np.linspace(-1, 1, num = img.shape[1])[np.newaxis, :]
mask = (ii ** (2*i+2) + jj ** (2*i+2)) ** (1.0 / (2*i+2)) < .75
masks.append(MaskData("Mask %d" % (i+1), mask,
def test_01_08_get_height(self):
file_name = os.path.join(self.root_dir, "ExampleSBSImages",
"Channel1-01-A-01.tif")
ip = I.load_imageplus(file_name)
target = load_image(file_name)
self.assertEqual(ip.getHeight(), target.shape[0])
def generate_2d_segmentation(sample):
excitations = ['488', '514', '561', '633']
image_name = ['{}_{}.czi'.format(sample, x) for x in excitations]
image_stack = [bioformats.load_image(filename) for filename in image_name]
image_sum = [np.sum(image, axis=2) for image in image_stack]
shift_vectors = [
skimage.feature.register_translation(np.log(image_sum[0] + 1e-4),
np.log(image_sum[i]))[0]
for i in range(1, 4)
]
shift_vectors.insert(0, np.asarray([0.0, 0.0]))
image_registered = [np.zeros(image.shape) for image in image_stack]
shift_filter_mask = [
np.full((image.shape[0], image.shape[1]), False, dtype=bool)
for image in image_stack
]
image_shape = image_stack[0].shape[0]
for i in range(len(image_stack)):
shift_row = int(shift_vectors[i][0])
shift_col = int(shift_vectors[i][1])
if np.abs(shift_row) > 15:
shift_row = 0
if np.abs(shift_col) > 15:
shift_col = 0
print(shift_row, shift_col)
original_row_min = int(np.maximum(0, shift_row))
original_row_max = int(image_shape + np.minimum(0, shift_row))
original_col_min = int(np.maximum(0, shift_col))
original_col_max = int(image_shape + np.minimum(0, shift_col))
registered_row_min = int(-np.minimum(0, shift_row))
registered_row_max = int(image_shape - np.maximum(0, shift_row))
registered_col_min = int(-np.minimum(0, shift_col))
registered_col_max = int(image_shape - np.maximum(0, shift_col))
image_registered[i][
original_row_min:original_row_max,
original_col_min:original_col_max, :] = image_stack[i][
registered_row_min:registered_row_max,
registered_col_min:registered_col_max, :]
shift_filter_mask[i][original_row_min:original_row_max,
original_col_min:original_col_max] = True
image_channel = np.dstack(image_registered)
image_registered_sum = np.sum(image_channel, axis=2)
image_registered_sum_norm = image_registered_sum / np.max(
image_registered_sum)
image_noise_variance = skimage.restoration.estimate_sigma(
image_registered_sum_norm)
image_registered_sum_nl = skimage.restoration.denoise_nl_means(
image_registered_sum_norm, h=0.02)
image_padded = skimage.util.pad(image_registered_sum_nl, 5, mode='edge')
image_lp = line_profile_2d_v2(image_padded.astype(np.float64), 11, 9)
image_lp = np.nan_to_num(image_lp)
image_lp_min = np.min(image_lp, axis=3)
image_lp_max = np.max(image_lp, axis=3)
image_lp_max = image_lp_max - image_lp_min
image_lp = image_lp - image_lp_min[:, :, :, None]
image_lp_rel_norm = image_lp / image_lp_max[:, :, :, None]
image_lp_rnc = image_lp_rel_norm[:, :, :, 5]
image_lprns = np.average(image_lp_rnc, axis=2)
image_lprn_lq = np.percentile(image_lp_rnc, 25, axis=2)
image_lprn_uq = np.percentile(image_lp_rnc, 75, axis=2)
image_lprn_qcv = np.zeros(image_lprn_uq.shape)
image_lprn_qcv_pre = (image_lprn_uq - image_lprn_lq) / (
image_lprn_uq + image_lprn_lq + 1e-8)
image_lprn_qcv[image_lprn_uq > 0] = image_lprn_qcv_pre[image_lprn_uq > 0]
image_final = image_lprns * (1 - image_lprn_qcv)
intensity_rough_seg = KMeans(n_clusters=2, random_state=0).fit_predict(
image_final.reshape(np.prod(image_final.shape),
1)).reshape(image_final.shape)
image0 = image_final * (intensity_rough_seg == 0)
image1 = image_final * (intensity_rough_seg == 1)
i0 = np.average(image0[image0 > 0])
i1 = np.average(image1[image1 > 0])
if (i0 < i1):
intensity_rough_seg_mask = (intensity_rough_seg == 1)
intensity_rough_seg_bkg = (intensity_rough_seg == 0)
else:
intensity_rough_seg_mask = (intensity_rough_seg == 0)
intensity_rough_seg_bkg = (intensity_rough_seg == 1)
image_lprns_rsfbo = skimage.morphology.binary_opening(
intensity_rough_seg_mask)
image_lprns_rsfbosm = skimage.morphology.remove_small_objects(
image_lprns_rsfbo, 10)
image_lprns_rsfbosm_bfh = binary_fill_holes(image_lprns_rsfbosm)
intensity_rough_seg_mask_bfh = binary_fill_holes(intensity_rough_seg_mask)
image_watershed_seeds = skimage.measure.label(image_lprns_rsfbosm_bfh *
intensity_rough_seg_mask_bfh)
image_registered_sum_nl_log = np.log10(image_registered_sum_nl)
image_bkg_filter = KMeans(n_clusters=2, random_state=0).fit_predict(
image_registered_sum_nl_log.reshape(
np.prod(image_registered_sum_nl_log.shape),
1)).reshape(image_registered_sum_nl_log.shape)
image0 = image_registered_sum_nl * (image_bkg_filter == 0)
image1 = image_registered_sum_nl * (image_bkg_filter == 1)
i0 = np.average(image0[image0 > 0])
i1 = np.average(image1[image1 > 0])
if (i0 < i1):
image_bkg_filter_mask = (image_bkg_filter == 1)
else:
image_bkg_filter_mask = (image_bkg_filter == 0)
image_final_bkg_filtered = image_registered_sum_nl_log * image_bkg_filter_mask
image_sum_bkg_filtered = image_registered_sum * image_bkg_filter_mask
image_watershed_seeds_bkg_filtered = image_watershed_seeds * image_bkg_filter_mask
image_watershed_sauvola = threshold_sauvola(image_registered_sum_nl)
image_watershed_mask_bkg_filtered = (
image_registered_sum_nl >
image_watershed_sauvola) * image_bkg_filter_mask
image_seg = skimage.morphology.watershed(
-image_final_bkg_filtered,
image_watershed_seeds_bkg_filtered,
mask=image_watershed_mask_bkg_filtered)
image_seg = skimage.segmentation.relabel_sequential(image_seg)[0]
adjacency_seg = skimage.morphology.watershed(
-image_sum_bkg_filtered,
image_watershed_seeds_bkg_filtered,
mask=image_bkg_filter_mask)
adjacency_seg = skimage.segmentation.relabel_sequential(adjacency_seg)[0]
save_segmentation(image_seg, sample)
return (image_registered_sum, image_channel, image_final_bkg_filtered,
image_seg, adjacency_seg)
def test_load_using_bioformats():
path = os.path.join(os.path.dirname(__file__), 'resources', 'Channel1-01-A-01.tif')
image, scale = bioformats.load_image(path, rescale=False, wants_max_intensity=True)
print(image.shape)
def z_and_t_area(path,meta_number=None, cycle_vm=True,crop_img=False,print_number=5,edge=False,binary=False,
image_channel=1,rescale_factor=255,meta_stage_loop=True,t_sample=1,show=True,
rebin=True,compute_height=False,canny_range=.33,
hist_cutoff=0.01,manual_thresh=False,m_thresh_high=1000,m_thresh_low=100):
# start javabridge
if cycle_vm:
javabridge.start_vm(class_path=bioformats.JARS)
# read in metadata using bioformats and make ararys for t ans z slices
metadata=v.extract_metadata(path,cycle_vm=False,meta_number=meta_number,stage_loop=meta_stage_loop)
z_slices=np.arange(0,metadata['size_Z'])
z_slices_scaled=z_slices*float(metadata['relative step width'])
t_slices=np.arange(0,metadata['size_T']-1)
t_slices=t_slices[::t_sample]
t_slices_scaled=t_slices*float(metadata['cycle time'])
# declare dataframes to store analysis results in
area_df=pd.DataFrame()
if compute_height:
height_df=pd.DataFrame()
# crop image option to do if specified
if crop_img:
# determine cropping bounds
img=bioformats.load_image(path=path,z=z_slices[3],t=t_slices[-1],series=0)
img = (img * rescale_factor).astype('uint8')
bounds = get_bounds(img,scale=0.4)
print_thresh=np.linspace(0,100,print_number)
print_count=0
plot_grid_count=1
finished_plotting=False
# iterate through time slices, stop before the last one
for i in range(len(t_slices)-1):
percent=t_slices[i]/(len(t_slices)-1)
if percent>print_thresh[print_count]:
# print('\tt: {:.2f} S'.format(t_slices_scaled[i]))
print_count+=1
# declare surface_area array outside of z-stack loop
area=np.zeros(len(z_slices))
for j in range(len(z_slices)):
# read image
img=bioformats.load_image(path=path,z=z_slices[j],t=t_slices[i],
rescale=False)
# if the image consists of multiple channels take just one channel
if len(np.shape(img))>2:
img=img[:,:,image_channel]
equalize_hist=exposure.equalize_adapthist(img,clip_limit=hist_cutoff,nbins=65535)
ratio=np.amax(equalize_hist)/65535
equalize_hist=np.round(equalize_hist/ratio,0).astype(int)
# if rebin is specified rebin image using 2x2 binning
if rebin:
equalize_hist=bin2d(img,2)
if crop_img:
crop=equalize_hist[int(bounds[1]):int(bounds[1] + bounds[3]), int(bounds[0]):int(bounds[0] + bounds[2])]
else:
crop=equalize_hist
# threshold image
if edge:
bw=edge_and_fill(crop,canny_range=canny_range)
else:
if manual_thresh:
bw=thresh_and_fill(img,low=m_thresh_low)
else:
bw=auto_threshold(img,'triangle')
# if compute height specified and on the firt step of loop make a zeros array
# the length and width of the image and height of the z_stacks number
if compute_height:
if j==0:
img_shape=np.shape(img)
img_stack=np.zeros([img_shape[0],img_shape[1],len(z_slices)])
img_stack[:,:,j]=bw
# subpart of program to show images comparing plots andbin
if show:
f1=plt.figure()
z_count=0
t_count=0
count_satisfied,t_count,z_count=show_handler(i,len(t_slices),
j,len(z_slices),
t_count,z_count)
if count_satisfied and not finished_plotting:
plt.subplot(6,6,plot_grid_count)
plt.imshow(equalize_hist)
plt.axis('off')
plt.subplot(6,6,plot_grid_count+1)
plt.imshow(bw)
plt.axis('off')
plot_grid_count+=2
if plot_grid_count>=36:
finished_plotting=True
f1.show()
# calculate area of thresholded image
if rebin:
scale=metadata['scale']**2
else:
scale=metadata['scale']**2
area[j]=get_area(bw,scale)
if compute_height:
average_height,length=calc_height(img_stack,scale,metadata['relative step width'])
loop_height=pd.DataFrame({
'Length (um)':length,
'Height (um)':average_height,
'time (s)':[t_slices_scaled[i]]*len(length)
})
height_df=height_df.append(loop_height,ignore_index=True)
# store in datafame along with other imporant variables
loop_area_df=pd.DataFrame({'Thrombus Area (um^2)':area,
'Z loc (um)':z_slices_scaled,
'time (s)':[t_slices_scaled[i]]*len(area)
})
area_df=area_df.append(loop_area_df,ignore_index=True)
if cycle_vm:
javabridge.kill_vm()
if compute_height:
return area_df,height_df
else:
return area_df
#### load z-stack
i = 0
nt = ox.image(i).Pixels.SizeT
nx = ox.image(i).Pixels.SizeX
ny = ox.image(i).Pixels.SizeY
nz = ox.image(i).Pixels.SizeZ
nc = ox.image(i).Pixels.SizeC
na = np.zeros((nt,nx,ny,nz,nc), dtype = 'uint8')
for it in range(nt):
for iz in range(nz):
na[it,:,:,iz,:] = bf.load_image(path, c=None, z=iz, t=it, series=i, index=None, rescale=False, wants_max_intensity=False, channel_names=None)
bg = np.zeros((nx,ny,nz), dtype = 'uint8')
viewer = vv.Viewer()
viewer.addGrayscaleLayer(bg, name="black")
green_layer = viewer.addAlphaModulatedLayer(na[0,...,0], name="green", tintColor=QColor(0,255,0))
blue_layer = viewer.addAlphaModulatedLayer(na[0,...,1], name="blue", tintColor=QColor(0,0,255))
green_layer.opacity = 1.0
blue_layer.opacity = 1.0
viewer.show()
#### load z-stack
def get_preview_image(fname, meta_xml=None, maxwh=256, series=0):
""" Generate a thumbnail of an image at the specified path. Gets the
middle Z plane of channel=0 and timepoint=0 of the specified series.
Parameters
----------
fname: str
Path to image file.
meta_xml: str
OME XML metadata.
maxwh: int
Maximum width or height of the output thumbnail. If the extracted
image is larger in either x or y, the image will will be downsized
to fit.
series: int
Series for multi-stack formats (default=0)
Returns
-------
img: numpy.ndarray
N x M array of grayscale pixel intentsities.
"""
name, ext = os.path.splitext(os.path.basename(fname))
if ext[1:] not in bioformats.READABLE_FORMATS:
raise Exception("Format not supported: %s" % ext[1:])
if not meta_xml:
meta_xml = bioformats.get_omexml_metadata(fname)
ome = bioformats.OMEXML(meta_xml)
if series > ome.get_image_count():
raise Exception(
"Specified series number %s exceeds number of series " "in the image file: %s" % (series, fname)
)
# Get metadata for first series
meta = ome.image(0).Pixels
# Determine resize factor
sizex = meta.SizeX
sizey = meta.SizeY
if sizex > maxwh or sizey > maxwh:
f = min(float(maxwh) / float(sizex), float(maxwh) / float(sizey))
else:
f = max(float(maxwh) / float(sizex), float(maxwh) / float(sizey))
# Determine which Z slice
z = 0
# Determine middle Z plane
# Note: SizeZ doesn't seem to be reliable. Might need to check BF.
# Stick with first slice for now.
# if meta.SizeZ > 1:
# z = int(math.floor(float(meta.SizeZ)/2))
# Load image plane
if meta.channel_count == 1 and meta.SizeC == 4:
# RGB Image
img = bioformats.load_image(fname, t=0, z=z, series=series, rescale=False)
return zoom(img, (f, f, 1))
else:
# Grayscale, grab channel 0 only
img = bioformats.load_image(fname, c=0, t=0, z=z, series=series, rescale=False)
# if img.dtype in (np.uint16, np.uint32, np.int16, np.int32):
img = stretch_contrast(img)
return zoom(img, f)
def get_preview_image(fname, meta_xml=None, maxwh=256, series=0):
""" Generate a thumbnail of an image at the specified path. Gets the
middle Z plane of channel=0 and timepoint=0 of the specified series.
Parameters
----------
fname: str
Path to image file.
meta_xml: str
OME XML metadata.
maxwh: int
Maximum width or height of the output thumbnail. If the extracted
image is larger in either x or y, the image will will be downsized
to fit.
series: int
Series for multi-stack formats (default=0)
Returns
-------
img: numpy.ndarray
N x M array of grayscale pixel intentsities.
"""
name, ext = os.path.splitext(os.path.basename(fname))
if ext[1:] not in bioformats.READABLE_FORMATS:
raise Exception("Format not supported: %s" % ext[1:])
if not meta_xml:
meta_xml = bioformats.get_omexml_metadata(fname)
ome = bioformats.OMEXML(meta_xml)
if series > ome.get_image_count():
raise Exception("Specified series number %s exceeds number of series "
"in the image file: %s" % (series, fname))
# Get metadata for first series
meta = ome.image(0).Pixels
# Determine resize factor
sizex = meta.SizeX
sizey = meta.SizeY
if sizex > maxwh or sizey > maxwh:
f = min(float(maxwh) / float(sizex), float(maxwh) / float(sizey))
else:
f = max(float(maxwh) / float(sizex), float(maxwh) / float(sizey))
# Determine which Z slice
z = 0
# Determine middle Z plane
# Note: SizeZ doesn't seem to be reliable. Might need to check BF.
# Stick with first slice for now.
# if meta.SizeZ > 1:
# z = int(math.floor(float(meta.SizeZ)/2))
# Load image plane
if meta.channel_count == 1 and meta.SizeC == 4:
# RGB Image
img = bioformats.load_image(fname,
t=0,
z=z,
series=series,
rescale=False)
return zoom(img, (f, f, 1))
else:
# Grayscale, grab channel 0 only
img = bioformats.load_image(fname,
c=0,
t=0,
z=z,
series=series,
rescale=False)
# if img.dtype in (np.uint16, np.uint32, np.int16, np.int32):
img = stretch_contrast(img)
return zoom(img, f)
def test_file_not_found(self):
# Regression test of issue #6
path = os.path.join(os.path.dirname(__file__), 'Channel5-01-A-01.tif')
self.assertRaises(exceptions.IOError,
lambda :load_image(path))
from __future__ import absolute_import, print_function, unicode_literals
import os
import javabridge
import bioformats
from bioformats import log4j
import sys
javabridge.start_vm(class_path=bioformats.JARS, run_headless=True)
try:
log4j.basic_config()
if len(sys.argv) < 2:
image_path = os.path.join(os.path.dirname(bioformats.__file__), '..',
'tests', 'resources', 'Channel1-01-A-01.tif')
else:
image_path = sys.argv[1]
image, scale = bioformats.load_image(image_path,
rescale=False,
wants_max_intensity=True)
try:
import pylab
pylab.imshow(image)
pylab.gca().set_title(image_path)
pylab.show()
except:
print(image.shape)
finally:
javabridge.kill_vm()
if __name__ == "__main__":
javabridge.start_vm(class_path=bioformats.JARS)
sequences = glob('./VoxelMorph/data/Series009*.tif')
print(len(sequences))
for name in sequences: # filter(lambda name: name.find('Seq') != -1, sequences)
def_x_name = '/home/nadya/Projects/VoxelMorph/data/deformations/ics/' \
+ name.split('/')[-1].split('.tif')[0] + '_ffXsh_fwd.ics'
def_y_name = '/home/nadya/Projects/VoxelMorph/data/deformations/ics/' \
+ name.split('/')[-1].split('.tif')[0] + '_ffYsh_fwd.ics'
seq = io.imread(name)
deform = np.empty(seq.shape + (2, ))
print(deform.shape)
print(name, def_x_name)
for i in tqdm(range(len(seq))):
deform[i, :, :, 0] = bioformats.load_image(def_x_name, z=i)[None]
deform[i, :, :, 1] = bioformats.load_image(def_y_name, z=i)[None]
tmp = deform[0].copy()
deform = deform[1:]
deform = deform[::-1]
deform = np.concatenate([tmp[None], deform], 0)
named = './VoxelMorph/data/deformations/numpy/' + name.split(
'/')[-1].split('.tif')[0] + '_fwd.npy'
np.save(named, deform)
def_x_name = './VoxelMorph/data/deformations/ics/' + name.split(
'/')[-1].split('.tif')[0] + '_ffXsh_bcw.ics'
def_y_name = './VoxelMorph/data/deformations/ics/' + name.split(
'/')[-1].split('.tif')[0] + '_ffYsh_bcw.ics'
def load_ztslice(filename, z_index, t_index):
image = bioformats.load_image(filename, z=z_index, t=t_index)
return (image)
def test_02_01_compare_to_matlab(self):
path = os.path.split(__file__)[0]
png_file = 'Channel2-01-A-01Mask.png'
mat_file = 'texturemeasurements.mat'
mask_file = os.path.join(path, png_file)
if not os.path.isfile(mask_file):
github_fmt = github_url + "/cellprofiler/modules/tests/%s"
mask = load_using_bioformats_url(github_fmt % png_file) != 0
fin = urllib2.urlopen(github_fmt % mat_file)
fd, tempmat = tempfile.mkstemp(".mat")
fout = os.fdopen(fd, "wb")
shutil.copyfileobj(fin, fout)
fout.close()
texture_measurements = loadmat(tempmat, struct_as_record=True)
os.remove(tempmat)
else:
mask = load_image(mask_file) != 0
texture_measurements = loadmat(
os.path.join(path, mat_file),
struct_as_record=True)
texture_measurements = texture_measurements['m'][0,0]
folder = 'ExampleSBSImages'
file_name = 'Channel1-01-A-01.tif'
image_file = os.path.join(
example_images_directory(), folder, file_name)
maybe_download_example_image([folder], file_name)
image = load_image(image_file, rescale=False).astype(float)/255.0
labels,count = scind.label(mask.astype(bool),np.ones((3,3),bool))
centers = scind.center_of_mass(np.ones(labels.shape), labels,
np.arange(count)+1)
centers = np.array(centers)
X = 1 # the index of the X coordinate
Y = 0 # the index of the Y coordinate
order_python = np.lexsort((centers[:,X],centers[:,Y]))
workspace, module = self.make_workspace(image, labels, convert = False)
module.scale_groups[0].scale.value = 3
my_angle = M.H_HORIZONTAL
module.scale_groups[0].angles.value = my_angle
module.run(workspace)
m = workspace.measurements
tm_center_x = texture_measurements['Location_Center_X'][0,0][:,0]
tm_center_y = texture_measurements['Location_Center_Y'][0,0][:,0]
order_matlab = np.lexsort((tm_center_x,tm_center_y))
for measurement in M.F_HARALICK:
mname = '%s_%s_%s_%d'%(M.TEXTURE, measurement, INPUT_IMAGE_NAME, 3)
pymname = mname + "_" + M.H_TO_A[my_angle]
pytm = m.get_current_measurement(INPUT_OBJECTS_NAME, pymname)
tm = texture_measurements[mname][0,0][:,]
error_count = 0
for i in range(count):
matlab_val = tm[order_matlab[i]]
python_val = pytm[order_python[i]]
self.assertAlmostEqual(tm[order_matlab[i]],
pytm[order_python[i]],7,
"Measurement = %s, Loc=(%.2f,%.2f), Matlab=%f, Python=%f"%
(mname, tm_center_x[order_matlab[i]],
tm_center_y[order_matlab[i]],
tm[order_matlab[i]],
pytm[order_python[i]]))
image_measurements =\
(('Texture_AngularSecondMoment_Cytoplasm_3', 0.5412),
('Texture_Contrast_Cytoplasm_3',0.1505),
('Texture_Correlation_Cytoplasm_3', 0.7740),
('Texture_Variance_Cytoplasm_3', 0.3330),
('Texture_InverseDifferenceMoment_Cytoplasm_3',0.9321),
('Texture_SumAverage_Cytoplasm_3',2.5684),
('Texture_SumVariance_Cytoplasm_3',1.1814),
('Texture_SumEntropy_Cytoplasm_3',0.9540),
('Texture_Entropy_Cytoplasm_3',1.0677),
('Texture_DifferenceVariance_Cytoplasm_3',0.1314),
('Texture_DifferenceEntropy_Cytoplasm_3',0.4147),
('Texture_InfoMeas1_Cytoplasm_3',-0.4236),
('Texture_InfoMeas2_Cytoplasm_3',0.6609))
for feature_name, value in image_measurements:
py_feature_name = feature_name + "_" + M.H_TO_A[my_angle]
pytm = m.get_current_image_measurement(py_feature_name)
self.assertAlmostEqual(pytm, value,3,
"%s failed. Python=%f, Matlab=%f" %
(feature_name, pytm, value))
def run_as_data_tool(self):
from cellprofiler.gui.editobjectsdlg import EditObjectsDialog
import wx
from wx.lib.filebrowsebutton import FileBrowseButton
from cellprofiler.modules.namesandtypes import ObjectsImageProvider
from bioformats import load_image
with wx.Dialog(None) as dlg:
dlg.Title = "Choose files for editing"
dlg.Sizer = wx.BoxSizer(wx.VERTICAL)
sub_sizer = wx.BoxSizer(wx.HORIZONTAL)
dlg.Sizer.Add(sub_sizer, 0, wx.EXPAND | wx.ALL, 5)
new_or_existing_rb = wx.RadioBox(dlg,
style=wx.RA_VERTICAL,
choices=("New", "Existing"))
sub_sizer.Add(new_or_existing_rb, 0, wx.EXPAND)
objects_file_fbb = FileBrowseButton(
dlg,
size=(300, -1),
fileMask=
"Objects file (*.tif, *.tiff, *.png, *.bmp, *.jpg)|*.tif;*.tiff;*.png;*.bmp;*.jpg",
dialogTitle="Select objects file",
labelText="Objects file:",
)
objects_file_fbb.Enable(False)
sub_sizer.AddSpacer(5)
sub_sizer.Add(objects_file_fbb, 0, wx.ALIGN_TOP | wx.ALIGN_RIGHT)
def on_radiobox(event):
objects_file_fbb.Enable(new_or_existing_rb.GetSelection() == 1)
new_or_existing_rb.Bind(wx.EVT_RADIOBOX, on_radiobox)
image_file_fbb = FileBrowseButton(
dlg,
size=(300, -1),
fileMask=
"Objects file (*.tif, *.tiff, *.png, *.bmp, *.jpg)|*.tif;*.tiff;*.png;*.bmp;*.jpg",
dialogTitle="Select guide image file",
labelText="Guide image:",
)
dlg.Sizer.Add(image_file_fbb, 0, wx.EXPAND | wx.ALL, 5)
allow_overlap_checkbox = wx.CheckBox(dlg, -1,
"Allow objects to overlap")
allow_overlap_checkbox.Value = True
dlg.Sizer.Add(allow_overlap_checkbox, 0, wx.EXPAND | wx.ALL, 5)
buttons = wx.StdDialogButtonSizer()
dlg.Sizer.Add(buttons, 0,
wx.ALIGN_CENTER_VERTICAL | wx.ALIGN_RIGHT | wx.ALL,
5)
buttons.Add(wx.Button(dlg, wx.ID_OK))
buttons.Add(wx.Button(dlg, wx.ID_CANCEL))
buttons.Realize()
dlg.Fit()
result = dlg.ShowModal()
if result != wx.ID_OK:
return
self.allow_overlap.value = allow_overlap_checkbox.Value
fullname = objects_file_fbb.GetValue()
guidename = image_file_fbb.GetValue()
if new_or_existing_rb.GetSelection() == 1:
provider = ObjectsImageProvider("InputObjects",
pathname2url(fullname), None, None)
image = provider.provide_image(None)
pixel_data = image.pixel_data
shape = pixel_data.shape[:2]
labels = [pixel_data[:, :, i] for i in range(pixel_data.shape[2])]
else:
labels = None
#
# Load the guide image
#
guide_image = load_image(guidename)
if np.min(guide_image) != np.max(guide_image):
guide_image = (guide_image - np.min(guide_image)) / (
np.max(guide_image) - np.min(guide_image))
if labels is None:
shape = guide_image.shape[:2]
labels = [np.zeros(shape, int)]
with EditObjectsDialog(guide_image, labels, self.allow_overlap,
self.object_name.value) as dialog_box:
result = dialog_box.ShowModal()
if result != wx.OK:
return
labels = dialog_box.labels
n_frames = len(labels)
with wx.FileDialog(None,
style=wx.FD_SAVE | wx.FD_OVERWRITE_PROMPT) as dlg:
dlg.Path = fullname
dlg.Wildcard = ("Object image file (*.tif,*.tiff)|*.tif;*.tiff|"
"Ilastik project file (*.ilp)|*.ilp")
result = dlg.ShowModal()
fullname = dlg.Path
if result == wx.ID_OK:
if fullname.endswith(".ilp"):
self.save_into_ilp(fullname, labels, guidename)
else:
from bioformats.formatwriter import write_image
from bioformats.omexml import PT_UINT16
if os.path.exists(fullname):
os.unlink(fullname)
for i, l in enumerate(labels):
write_image(fullname,
l,
PT_UINT16,
t=i,
size_t=len(labels))
def load_img(path, series, z_max):
"""gets all z-stacks in an image"""
all_z_in_image = [
bioformats.load_image(path, series=series, z=z) for z in range(z_max)
]
return (all_z_in_image)
def test_01_08_get_height(self):
file_name = os.path.join(
self.root_dir, "ExampleSBSImages", "Channel1-01-A-01.tif")
ip = I.load_imageplus(file_name)
target = load_image(file_name)
self.assertEqual(ip.getHeight(), target.shape[0])
def run_as_data_tool(self):
from cellprofiler.gui.editobjectsdlg import EditObjectsDialog
import wx
from wx.lib.filebrowsebutton import FileBrowseButton
from cellprofiler.modules.namesandtypes import ObjectsImageProvider
from bioformats import load_image
with wx.Dialog(None) as dlg:
dlg.Title = "Choose files for editing"
dlg.Sizer = wx.BoxSizer(wx.VERTICAL)
box = wx.StaticBox(dlg, -1, "Choose or create new objects file")
sub_sizer = wx.StaticBoxSizer(box, wx.HORIZONTAL)
dlg.Sizer.Add(sub_sizer, 0, wx.EXPAND | wx.ALL, 5)
new_or_existing_rb = wx.RadioBox(dlg, style=wx.RA_VERTICAL,
choices = ("New", "Existing"))
sub_sizer.Add(new_or_existing_rb, 0, wx.EXPAND)
objects_file_fbb = FileBrowseButton(
dlg, size=(300, -1),
fileMask="Objects file (*.tif, *.tiff, *.png, *.bmp, *.jpg)|*.tif;*.tiff;*.png;*.bmp;*.jpg",
dialogTitle="Select objects file",
labelText="Objects file:")
objects_file_fbb.Enable(False)
sub_sizer.AddSpacer(5)
sub_sizer.Add(objects_file_fbb, 0, wx.ALIGN_TOP | wx.ALIGN_RIGHT)
def on_radiobox(event):
objects_file_fbb.Enable(new_or_existing_rb.GetSelection() == 1)
new_or_existing_rb.Bind(wx.EVT_RADIOBOX, on_radiobox)
image_file_fbb = FileBrowseButton(
dlg, size=(300, -1),
fileMask="Objects file (*.tif, *.tiff, *.png, *.bmp, *.jpg)|*.tif;*.tiff;*.png;*.bmp;*.jpg",
dialogTitle="Select guide image file",
labelText="Guide image:")
dlg.Sizer.Add(image_file_fbb, 0, wx.EXPAND | wx.ALL, 5)
allow_overlap_checkbox = wx.CheckBox(dlg, -1, "Allow objects to overlap")
allow_overlap_checkbox.Value = True
dlg.Sizer.Add(allow_overlap_checkbox, 0, wx.EXPAND | wx.ALL, 5)
buttons = wx.StdDialogButtonSizer()
dlg.Sizer.Add(buttons, 0, wx.ALIGN_CENTER_VERTICAL | wx.ALIGN_RIGHT | wx.ALL, 5)
buttons.Add(wx.Button(dlg, wx.ID_OK))
buttons.Add(wx.Button(dlg, wx.ID_CANCEL))
buttons.Realize()
dlg.Fit()
result = dlg.ShowModal()
if result != wx.ID_OK:
return
self.allow_overlap.value = allow_overlap_checkbox.Value
fullname = objects_file_fbb.GetValue()
guidename = image_file_fbb.GetValue()
if new_or_existing_rb.GetSelection() == 1:
provider = ObjectsImageProvider(
"InputObjects",
pathname2url(fullname),
None, None)
image = provider.provide_image(None)
pixel_data = image.pixel_data
shape = pixel_data.shape[:2]
labels = [pixel_data[:, :, i] for i in range(pixel_data.shape[2])]
else:
labels = None
#
# Load the guide image
#
guide_image = load_image(guidename)
if np.min(guide_image) != np.max(guide_image):
guide_image = ((guide_image - np.min(guide_image)) /
(np.max(guide_image) - np.min(guide_image)))
if labels is None:
shape = guide_image.shape[:2]
labels = [np.zeros(shape, int)]
with EditObjectsDialog(
guide_image, labels,
self.allow_overlap, self.object_name.value) as dialog_box:
result = dialog_box.ShowModal()
if result != wx.OK:
return
labels = dialog_box.labels
n_frames = len(labels)
with wx.FileDialog(None,
style = wx.FD_SAVE | wx.FD_OVERWRITE_PROMPT) as dlg:
dlg.Path = fullname
dlg.Wildcard = ("Object image file (*.tif,*.tiff)|*.tif;*.tiff|"
"Ilastik project file (*.ilp)|*.ilp")
result = dlg.ShowModal()
fullname = dlg.Path
if result == wx.ID_OK:
if fullname.endswith(".ilp"):
self.save_into_ilp(fullname, labels, guidename)
else:
from bioformats.formatwriter import write_image
from bioformats.omexml import PT_UINT16
if os.path.exists(fullname):
os.unlink(fullname)
for i, l in enumerate(labels):
write_image(fullname, l, PT_UINT16,
t = i, size_t = len(labels))
def test_file_not_found():
# Regression test of issue #6
path = os.path.join(os.path.dirname(__file__), 'resources', 'Channel5-01-A-01.tif')
with pytest.raises(IOError):
bioformats.load_image(path)
#!/usr/bin/env python
import os
import javabridge
import bioformats
from bioformats import log4j
import sys
javabridge.start_vm(class_path=bioformats.JARS,
run_headless=True)
try:
log4j.basic_config()
if len(sys.argv) < 2:
image_path = os.path.join(os.path.dirname(bioformats.__file__), 'tests',
'Channel1-01-A-01.tif')
else:
image_path = sys.argv[1]
image, scale = bioformats.load_image(image_path, rescale=False,
wants_max_intensity=True)
try:
import pylab
pylab.imshow(image)
pylab.gca().set_title(image_path)
pylab.show()
except:
print image.shape
finally:
javabridge.kill_vm()