def movetoch4():
fluidics.stop_flow()
stage.move_to("J1")
stage.move_to("Ch1")
setpressure(0.1)
fluidics.open(1, 2, 3, 4, 6)
reset_force()
print("setup should be ready to go")
def thelooploop(): #return 1 if done
chk = 0
while chk == 0:
print("starting loop loop")
chk = themainloop()
if chk == 1:
chk = false_positive()
else:
target_traps = traps[:2]
target_traps[1].clear()
target_traps[0].clear()
#hopefully caught something, move to buffer channel and reset all
stage.move_to("buffer")
gohome()
fluidics.stop_flow()
reset_force()
print("in the buffer channel with all reset")
#0 for no ssDNA, 1 for good, 2 for failed to pull
if makessDNA == 1:
print("time to try for ssDNA")
s = 0
while s != 1:
s = ssDNAstretch()
if s == 0:
setpressure(0.3)
fluidics.open(3, 6)
target_traps = traps[:2]
target_traps[1].clear()
target_traps[0].clear()
fluidics.close(3, 6)
return s
if s == 2:
continue
else:
return 1
while fluidics.pressure < pres:
fluidics.increase_pressure()
pause(0.1) #important!
else:
while fluidics.pressure > pres:
fluidics.decrease_pressure()
pause(0.1) #important!
def timeprogression(time, numsteps):
stepsize = round(time / numsteps, 2)
for i in range(1, numsteps + 1):
pause(stepsize)
print(" " + str(round(i * stepsize * 100 / time)) +
"% of time has elapsed (" + str(round(i * stepsize / 60)) +
" min)")
#MAIN ------------------------------------------
print("starting high pressure")
setpressure(1.4)
fluidics.open(1, 2, 3, 4, 5, 6)
timeprogression(60 * 3, 3)
print("high done, starting low pressure")
setpressure(0.4)
timeprogression(60 * 27, 14)
fluidics.stop_flow()
print(" should be done and vented")