def compute_stats(values, concentrations, background_std, clean=True):
def preprocess_concentrations():
u_concentrations = np.unique(concentrations)[1:]
re_concentrations = np.log(u_concentrations)
_5_p = np.log(1.05)
backbone = squareform(pdist(re_concentrations[:, np.newaxis]))
msk = np.array((backbone < _5_p).nonzero()).T
collapse = []
for i, j in msk.tolist():
if i > j:
collapse.append((u_concentrations[i], u_concentrations[j]))
for c1, c2 in collapse:
concentrations[concentrations == c2] = c1
preprocess_concentrations()
unique_values = np.unique(concentrations)
means = np.zeros_like(unique_values)
errs = np.zeros_like(unique_values)
stds = np.zeros_like(unique_values)
freedom_degs = np.zeros_like(unique_values)
for i, val in enumerate(unique_values):
mask = concentrations == val
vals = rm_nans(values[:, mask, :])
means[i] = np.mean(vals)
stds[i] = np.sqrt(np.std(vals) ** 2 + background_std ** 2)
freedom_degs[i] = np.max((vals.shape[0] - 1, 1))
errs[i] = get_t_distro_outlier_bound_estimation(vals, background_std) / freedom_degs[i]
return means, errs, stds, freedom_degs, unique_values
def preformat(means_accumulator, errs_accumulator, all_cell_lines_arr, names_accumulator):
means_accumulator = means_accumulator.tolist()
errs_accumulator = errs_accumulator.tolist()
all_cell_lines = np.sort(all_cell_lines_arr).tolist()
idx1 = all_cell_lines.index("184A1")
idx2 = all_cell_lines.index("184B5")
mean_for_proxy_wt = np.nanmean(np.array(means_accumulator)[[idx1, idx2], :], axis=0)
errs_for_proxy_wt = np.nanmean(np.array(errs_accumulator)[[idx1, idx2], :], axis=0)
all_cell_lines.append("WT_proxy")
means_accumulator.append(mean_for_proxy_wt.tolist())
errs_accumulator.append(errs_for_proxy_wt.tolist())
means_accumulator = np.array(means_accumulator)
errs_accumulator = np.array(errs_accumulator)
support = np.logical_not(np.isnan(means_accumulator[-1, :]))
names_accumulator = np.array(names_accumulator)
all_cell_lines_arr = np.array(all_cell_lines)
tmp_calc = rm_nans(means_accumulator)
q1 = np.percentile(tmp_calc, 25)
q3 = np.percentile(tmp_calc, 75)
ub = q3 + (q3 - q1) * 1.5
means_accumulator[means_accumulator > ub] = np.nan
errs_accumulator[means_accumulator > ub] = np.nan
means_accumulator = means_accumulator[:, support]
errs_accumulator = errs_accumulator[:, support]
names_accumulator = names_accumulator[support]
line_wise_support = np.sum(np.logical_not(np.isnan(means_accumulator)), axis=1)
cell_lines_support_filter = line_wise_support > 10
ban_list = ["MCF10A", "MCF10F", "MCF12A"]
for item in ban_list:
idx = all_cell_lines.index(item)
cell_lines_support_filter[idx] = False
means_accumulator = means_accumulator[cell_lines_support_filter, :]
errs_accumulator = errs_accumulator[cell_lines_support_filter, :]
all_cell_lines_arr = all_cell_lines_arr[cell_lines_support_filter]
column_wise_support = np.sum(np.logical_not(np.isnan(means_accumulator)), axis=0)
drugs_support_filter = column_wise_support > 20
means_accumulator = means_accumulator[:, drugs_support_filter]
errs_accumulator = errs_accumulator[:, drugs_support_filter]
names_accumulator = names_accumulator[drugs_support_filter]
return means_accumulator, errs_accumulator, all_cell_lines_arr, names_accumulator
def get_t_distro_outlier_bound_estimation(array, background_std):
nums_only_array = rm_nans(array)
low, up = t.interval(
0.95,
nums_only_array.shape[0] - 1,
np.mean(nums_only_array),
np.sqrt(np.var(nums_only_array) + background_std ** 2),
)
up, low = (up - np.mean(nums_only_array), np.mean(nums_only_array) - low)
return max(up, low)
def errplot_with_selectors(table, errtable):
for i in range(0, len(selector)):
v1 = table.reset_index().values[:, 1:][i, :].flatten()
v2 = errtable.reset_index().values[:, 1:][i, :].flatten()
v1 = v1.astype(np.float)
nl = np.sum(np.logical_not(np.isnan(v1)))
gni = gini_coeff(1./rm_nans(v1))
plt.errorbar(condition_index, v1, v2, fmt='.', label='%s; gini: %.2f, valid: %s' %
(pre_selector[i], gni, nl))
plt.xticks(condition_index, condition_names, rotation='vertical')
plt.gca().set_yscale("log", nonposy='clip')
plt.legend(loc='upper left', prop={'size': 10})
plt.show()
def logistic_regression(tf, t0, concentrations, background_std):
def get_1p_bounds(mean, std, dof):
return t.interval(0.99, dof, mean, std)
mask = concentrations == 0.0
vals_at_conc_0 = rm_nans(tf[:, mask, :])
max_capacity = (
get_1p_bounds(
np.mean(vals_at_conc_0), np.sqrt(np.var(vals_at_conc_0) + background_std ** 2), vals_at_conc_0.shape[0]
)[1]
* 1.05
)
compensation_t0 = -np.log2(max_capacity / t0 - 1)[:, :, np.newaxis]
compensation_tf = -np.log2(max_capacity / tf - 1)
alphas = compensation_tf - compensation_t0
return alphas
def __init__(self, pth, fle, alpha_bound_percentile=5):
cells = []
drugs = []
drug_versions = defaultdict(list)
plates = []
with open(path.join(pth, fle)) as src:
rdr = reader(src, dialect='excel-tab')
header = rdr.next()
for row in rdr:
expanded_drug_name = (row[1], float(row[47]))
cells.append(row[0])
drug_versions[row[1]].append(expanded_drug_name)
drugs.append(expanded_drug_name)
plates.append(row[2])
cell_idx = supporting_functions.index(set(cells))
drug_idx = supporting_functions.index(set(drugs))
plates_idx = supporting_functions.index(set(plates))
drug_versions = dict([(key, list(set(values)))
for key, values in drug_versions.iteritems()])
cell_idx_rv = dict([(value, key) for key, value in cell_idx.iteritems()])
drug_idx_rv = dict([(value, key) for key, value in drug_idx.iteritems()])
plates_idx_rv = dict([(value, key) for key, value in plates_idx.iteritems()])
cells_no = len(cell_idx)
drugs_no = len(drug_idx)
plates_no = len(plates_idx)
depth_limiter = 7
storage = np.empty((cells_no, drugs_no, depth_limiter, 10, 3))
storage.fill(np.NaN)
background = np.empty((cells_no, drugs_no, depth_limiter, 4))
background.fill(np.NaN)
t0_median = np.empty((cells_no, drugs_no, depth_limiter))
t0_median.fill(np.NaN)
t0_background = np.empty((cells_no, drugs_no, depth_limiter))
t0_background.fill(np.NaN)
tf_background = np.empty((cells_no, drugs_no, depth_limiter))
tf_background.fill(np.NaN)
background_noise = np.empty((plates_no, 2))
background_noise.fill(np.NaN)
cl_drug_replicates = np.zeros((cells_no, drugs_no))
with open(path.join(pth, fle)) as src:
rdr = reader(src, dialect='excel-tab')
test_array = rdr.next()
supporting_functions.broadcast(test_array[6:36])
for row in rdr:
cell_no = cell_idx[row[0]]
drug_no = drug_idx[(row[1], float(row[47]))]
plate_no = plates_idx[row[2]]
depth_index = min(cl_drug_replicates[cell_no, drug_no], depth_limiter-1)
storage[cell_no, drug_no, depth_index, :, :] = supporting_functions.broadcast(row[6:36])
background[cell_no, drug_no, depth_index, :] = supporting_functions.lgi(row, [4, 5, 36, 37])
t0_median[cell_no, drug_no, depth_index] = row[38]
t0_background[cell_no, drug_no, depth_index] = np.mean(
supporting_functions.lgi(row, [4, 5]).astype(np.float64)).tolist()
tf_background[cell_no, drug_no, depth_index] = np.mean(
supporting_functions.lgi(row, [36, 37]).astype(np.float64)).tolist()
background_noise[plate_no, :] = np.abs(
supporting_functions.lgi(row, [4, 36]).astype(np.float64) -
supporting_functions.lgi(row, [5, 37]).astype(
np.float64))
cl_drug_replicates[cell_no, drug_no] += 1
cl_drug_replicates[cl_drug_replicates < 1] = np.nan
alpha_bound = np.percentile(rm_nans(background_noise), 100 - alpha_bound_percentile)
std_of_tools = np.percentile(rm_nans(background_noise), 66)
background = supporting_functions.p_stabilize(background, 0.5)
t0_background = supporting_functions.p_stabilize(t0_background, 0.5)
tf_background = supporting_functions.p_stabilize(tf_background, 0.5)
storage_dblanc = storage - tf_background[:, :, :, np.newaxis, np.newaxis]
self.header_line = header
self.cell_line_2_idx = cell_idx
self.drug_2_idx = drug_idx
self.idx_2_cell_line = cell_idx_rv
self.idx_2_drug = drug_idx_rv
self.raw_data = storage_dblanc # cell_line, drug, concentration -> 3 replicates
self.background = background # cell_line, drug -> (T0_1, T0_2, T_final, T_final) backgrounds
self.t0_background = t0_background
self.t_f_background = tf_background
self.t0_median = t0_median # for each cell_line and drug contains T0
self.alpha_bound = alpha_bound # lower significance bound
self.std_of_tools = std_of_tools
self.background_noise = background_noise
self.drug_versions = dict(drug_versions) # drug names + concentrations versions
self.cl_drug_replicates = cl_drug_replicates
def p_stabilize(array, percentile):
p_low = np.percentile(rm_nans(array), percentile)
p_high = np.percentile(rm_nans(array), 100 - percentile)
array[array < p_low] = p_low
array[array > p_high] = p_high
return array
def retrieve_normalization_factor(T0_median_array):
redux_function = lambda x: rm_nans(x)[0]
retour = np.apply_along_axis(redux_function, 1, T0_median_array)
return retour
def quick_hist(data):
plt.hist(np.log10(rm_nans(data)), bins=20)
plt.show()
