def HTSeqCount(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
gtf = configRobot.popParas(cmdset, ['GTF'])
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
for sample in samples:
paraset = copy.deepcopy(cmdset)
jobfnprefix = prefix + '_' + sample
if bam == '=sample':
inputfile = sample
outputfile = sample + '.count'
else:
inputfile = sample + '/' + bam
outputfile = sample + '/' + bam + '.count'
cmdGenerator.checkPath(outputpath + sample, create=createpath)
samcmd = 'samtools view -h %s | '%(inputpath+inputfile)
htseq = 'python -m HTSeq.scripts.count -q '
countcmd = cmdGenerator.formatCmd(samcmd, htseq, paraset, '-', gtf, ' > %s'%(outputpath + outputfile))
mvscriptcmd = cmdGenerator.formatCmd('mv ./%s%s %s'%(jobfnprefix, jobmanager.ext, outputpath))
jobmanager.createJob(jobfnprefix, [setuppathcmd, countcmd, mvscriptcmd], outpath = outputpath, outfn = jobfnprefix)
return jobmanager
def gmfit(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, call, mem, time, prefix, Ks, datafn = configRobot.popParas(cmdset, ['cmd', 'call', 'mem', 'time', 'prefix', 'Ks', 'datafn'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
cmdGenerator.checkPath(outputpath, create=createpath)
if type(Ks) != type([]):
Ks = [Ks]
for fn in datafn:
for k in Ks:
jobname = prefix + fn.replace('.mat', '') + 'k%03d'%(int(k))
functionCall = call + "('%s', %s, '%s');"%(inputpath+fn, k, outputpath + jobname + '.mat')
CMD = []
CMD.append( cmdGenerator.formatCmd( matlabcmd%(functionCall) ) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def cuffcompare(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, gtf = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'gtf'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
sampletext = ''
for sample in samples:
sampletext = sampletext + '%s%s/%s '%(inputpath, sample, gtf)
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
jobname = prefix
CMD = []
CMD.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outputpath + paraset['-o']
paraset = configRobot.validParas(paraset, availParas['cuffcompare'])
CMD.append( cmdGenerator.formatCmd(cmd, paraset, sampletext) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def markDup(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(cmdset,['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
javacmd = 'java -Xmx%dg -jar'%(int(mem.replace('G',''))-1)
mdupjar = 'MarkDuplicates.jar'
idxcmd = 'samtools index'
for sample in samples:
jobname = prefix + '_' + sample
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s/%s'%(inputpath + sample, bam)
paraset['OUTPUT'] = paraset['INPUT'].replace('.bam', '.mdup.bam')
paraset['METRICS_FILE'] = '=%s/%s'%(inputpath + sample, prefix + '_mdupmetrics.txt')
paraset = configRobot.validParas(paraset, availParas[mdupjar])
CMDs = []
CMDs.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
CMDs.append( cmdGenerator.formatCmd(javacmd, programpath+mdupjar, paraset) )
CMDs.append( cmdGenerator.formatCmd(idxcmd, paraset['OUTPUT'].strip('=')) )
CMDs.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, inputpath+sample)) )
jobmanager.createJob(jobname, CMDs, outpath = inputpath+sample, outfn = jobname)
return jobmanager
def RSeQC(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time = configRobot.popParas(cmdset, ['cmd', 'mem', 'time'])
samples, bam, prefix = configRobot.popParas(cmdset, ['sample', 'bam', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
programs = ['inner_distance.py', 'junction_annotation.py', 'junction_saturation.py', 'read_GC.py', 'read_duplication.py']
for sample in samples:
jobname = prefix + '_' + sample
CMDs = []
CMDs.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
for prog in programs:
paraset = copy.deepcopy(cmdset)
paraset['-i'] = inputpath + sample + '/' + bam
paraset['-o'] = outputpath + sample + '.%s'%(prog.replace('.py', ''))
paraset = configRobot.validParas(paraset, availParas[prog])
if '-o' not in paraset.keys():
paraset['>'] = outputpath + sample + '.%s'%(prog.replace('.py', ''))
CMDs.append( cmdGenerator.formatCmd('python', programpath+prog, paraset) )
CMDs.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMDs, outpath = outputpath, outfn = jobname)
return jobmanager
def gseaTestAnt2Pheno(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, call, mem, time, prefix, datasets, phenos, features, njobs = configRobot.popParas(cmdset, ['cmd', 'call', 'mem', 'time', 'prefix', 'dataset', 'pheno', 'feature', 'njob'])
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
cmdGenerator.checkPath(outputpath, create=createpath)
for di in range(len(datasets)):
numjobs = int(njobs[di])
if datasets[di] != 'CCLE':
continue
for jobidx in range(1, numjobs+1):
jobname = prefix + datasets[di] + '%02d'%jobidx
functionCall = call + "(%d, %d, '%s', 'dataset', '%s', 'pheno', '%s', 'feature', '%s');"%(jobidx, numjobs, outputpath + prefix, datasets[di], phenos[di], features[di])
CMD = []
CMD.append( cmdGenerator.formatCmd( matlabcmd%(functionCall) ) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def cuffmerge(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, gtf = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'gtf'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
sampletext = '"'
for sample in samples:
sampletext = sampletext + '%s%s/%s\\n' % (inputpath, sample, gtf)
sampletext = sampletext + '"'
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
jobname = prefix
CMD = []
CMD.append(cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outputpath + paraset['-o']
CMD.append(
cmdGenerator.formatCmd('echo', sampletext, '>',
paraset['-o'] + '.samples'))
paraset = configRobot.validParas(paraset, availParas['cuffmerge'])
cmdGenerator.checkPath(paraset['-o'], create=createpath)
CMD.append(cmdGenerator.formatCmd(cmd, paraset,
paraset['-o'] + '.samples'))
CMD.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, paraset['-o'])))
CMD.append(cmdGenerator.formatCmd('rm -f', paraset['-o'] + '.samples'))
sgeopt = []
if '-p' in paraset.keys():
if int(paraset['-p']) > 1: #multi threads
sgeopt = ['-pe smp ' + paraset['-p']]
elif '--num-threads' in paraset.keys():
if int(paraset['--num-threads']) > 1:
sgeopt = ['-pe smp ' + paraset['-p']]
jobmanager.createJob(jobname,
CMD,
outpath=paraset['-o'],
outfn=jobname,
sgeopt=sgeopt,
trackcmd=False)
return jobmanager
def tophat(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix'])
paired = configRobot.popParas(cmdset, ['paired'])
readext, outpath, genome = configRobot.popParas(
cmdset, ['readext', 'outputpath', 'genome'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd(
'source ~/libraries/setup_seqtools\necho $BOWTIE2_INDEXES')
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
cmdGenerator.checkPath(outpath + '%s/' % prefix, create=createpath)
for sample in samples:
if paired == 'paired' or paired == 'yes':
reads = map(lambda (i): inputpath + sample + '_%d' % i + readext,
[1, 2])
elif paired == 'single' or paired == 'no':
reads = inputpath + sample + readext
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outpath + '%s/' % prefix + sample
jobfnprefix = prefix + '_' + sample
tophatcmd = cmdGenerator.formatCmd(cmd, paraset, genome, reads)
mvscriptcmd = cmdGenerator.formatCmd(
'mv ./%s%s %s' % (jobfnprefix, jobmanager.ext, paraset['-o']))
if int(paraset['-p']) > 1: #multiple threads per job
sgeopt = ['-pe smp ' + paraset['-p']]
else:
sgeopt = []
#need to create the output directory first, otherwise SGE complains cannot put the stdout in its path
cmdGenerator.checkPath(paraset['-o'], create=createpath)
jobmanager.createJob(jobfnprefix,
[setuppathcmd, tophatcmd, mvscriptcmd],
outpath=paraset['-o'],
outfn=jobfnprefix,
sgeopt=sgeopt)
return jobmanager
def DESeqPair(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmdset = configRobot.makeParasList(cmdset, ['meta', 'group1', 'group2'])
cmd, mem, time, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'prefix'])
group1, group2 = configRobot.popParas(cmdset, ['group1', 'group2'])
template = open(cmdset.pop('template')).read()
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
cmdset['inputpath'] = inputpath
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
cmdset['outputpath'] = outputpath
cmdset['prefix'] = prefix
meta = configRobot.popParas(cmdset, ['meta'])
if meta[0] == "''" and len(meta) == 1:
cmdset['meta'] = 'c()'
else:
cmdset['meta'] = 'c(\'' + '\', \''.join(meta) + '\')'
if cmdset['countfnprefix'] == "''": cmdset['countfnprefix'] = ''
if cmdset['countfnsuffix'] == "''": cmdset['countfnsuffix'] = ''
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
for i in range(len(group1)):
paraset = copy.deepcopy(cmdset)
paraset['gr1'] = group1[i]
paraset['gr2'] = group2[i]
jobfnprefix = prefix + '_' + group1[i] + '_' + group2[i]
f = open('./%s.R' % (jobfnprefix), 'w')
f.write(template % paraset)
f.close()
deseqcmd = cmdGenerator.formatCmd('Rscript', './%s.R' % (jobfnprefix))
mvRscriptcmd = cmdGenerator.formatCmd('mv ./%s.R %s' %
(jobfnprefix, outputpath))
mvscriptcmd = cmdGenerator.formatCmd(
'mv ./%s%s %s' % (jobfnprefix, jobmanager.ext, outputpath))
jobmanager.createJob(
jobfnprefix, [setuppathcmd, deseqcmd, mvRscriptcmd, mvscriptcmd],
outpath=outputpath,
outfn=jobfnprefix)
return jobmanager
def cuffdiff_v1(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, gtf, bam = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'gtf', 'bam'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
sampletext = ''
for sample in samples:
sampletext = sampletext + '%s%s/%s ' % (inputpath, sample, bam)
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
jobname = prefix
CMD = []
CMD.append(cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
paraset = copy.deepcopy(cmdset)
paraset = configRobot.validParas(paraset, availParas['cuffdiff'])
cmdGenerator.checkPath(paraset['--output-dir'], create=createpath)
CMD.append(
cmdGenerator.formatCmd(
'/ifs/home/c2b2/dp_lab/bc2252/SeqTool/cufflinks_1/cuffdiff',
paraset, gtf, sampletext))
CMD.append(
cmdGenerator.formatCmd(
'mv ./%s%s %s' %
(jobname, jobmanager.ext, paraset['--output-dir'])))
sgeopt = []
if '-p' in paraset.keys():
if int(paraset['-p']) > 1: #multi threads
sgeopt = ['-pe smp ' + paraset['-p']]
elif '--num-threads' in paraset.keys():
if int(paraset['--num-threads']) > 1:
sgeopt = ['-pe smp ' + paraset['-p']]
jobmanager.createJob(jobname,
CMD,
outpath=paraset['--output-dir'],
outfn=jobname,
sgeopt=sgeopt,
trackcmd=False)
return jobmanager
def picardReorderSam(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(
cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar' % (int(mem.replace('G', '')) - 2)
reorder = programpath + 'ReorderSam.jar VALIDATION_STRINGENCY=LENIENT'
for sample in samples:
CMDs = []
CMDs.append(
cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
jobname = prefix + '_' + sample
#reorder by chrm
paraset = copy.deepcopy(cmdset)
if bam == '=sample':
inputfile = sample
else:
inputfile = sample + '/' + bam
paraset['INPUT'] = '=%s' % (inputpath + inputfile)
paraset['OUTPUT'] = '=%s.reorder.bam' % (outputpath +
inputfile.replace('.bam', ''))
paraset = configRobot.validParas(paraset, availParas['ReorderSam.jar'])
CMDs.append(cmdGenerator.formatCmd(javacmd, reorder, paraset))
CMDs.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, outputpath)))
jobmanager.createJob(jobname, CMDs, outpath=outputpath, outfn=jobname)
return jobmanager
def RNASeQC(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time = configRobot.popParas(cmdset, ['cmd', 'mem', 'time'])
samples, bam, prefix = configRobot.popParas(cmdset,
['sample', 'bam', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(
cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar' % (int(mem.replace('G', '')) - 2)
#need to generate -s, -o
#generate a temperory file
samplestr = '"Sample ID\\tBam File\\tNotes\\n'
for sample in samples:
samplestr = samplestr + '%s\\t%s\\t%s\\n' % (
sample, inputpath + sample + '/' + bam, sample)
samplestr = samplestr + '"'
samplefile = '%s.samples' % prefix
cmdset['-s'] = samplefile
cmdset['-o'] = inputpath + 'RNA-SeQC/'
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
setupcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
createsamplefile = cmdGenerator.formatCmd('echo', samplestr, '>',
samplefile)
removesamplefile = cmdGenerator.formatCmd('rm -f', samplefile)
mvscriptcmd = cmdGenerator.formatCmd(
'mv %s%s %s' % (prefix, jobmanager.ext, cmdset['-o']))
qccmd = cmdGenerator.formatCmd(javacmd, programpath + cmd, cmdset)
cmdGenerator.checkPath(cmdset['-o'], create=createpath)
jobmanager.createJob(
prefix,
[setupcmd, createsamplefile, qccmd, removesamplefile, mvscriptcmd],
outfn=prefix,
outpath=cmdset['-o'],
trackcmd=False)
return jobmanager
def cufflinks(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
outputpath = cmdGenerator.checkPath(outputpath + '%s/' % prefix,
create=createpath)
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
for sample in samples:
jobname = prefix + '_' + sample
CMD = []
CMD.append(cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outputpath + sample
paraset = configRobot.validParas(paraset, availParas['cufflinks'])
cmdGenerator.checkPath(paraset['-o'], create=createpath)
CMD.append(
cmdGenerator.formatCmd(cmd, paraset,
inputpath + '%s/' % sample + bam))
CMD.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, paraset['-o'])))
sgeopt = []
if '-p' in paraset.keys():
if int(paraset['-p']) > 1: #multi threads
sgeopt = ['-pe smp ' + paraset['-p']]
elif '--num-threads' in paraset.keys():
if int(paraset['--num-threads']) > 1:
sgeopt = ['-pe smp ' + paraset['-p']]
jobmanager.createJob(jobname,
CMD,
outpath=paraset['-o'],
outfn=jobname,
sgeopt=sgeopt)
return jobmanager
def picardQC(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, bam, prefix = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'bam', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
samples = cmdset.pop('sample')
javacmd = 'java -Xmx%dg -jar'%(int(mem.replace('G',''))-2)
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
metrics = {'CollectRnaSeqMetrics.jar': 'RnaSeq', 'CollectMultipleMetrics.jar': '', 'EstimateLibraryComplexity.jar': 'Lib', 'CollectGcBiasMetrics.jar': 'GC'}
metrickeys = ['CollectRnaSeqMetrics.jar', 'CollectMultipleMetrics.jar', 'EstimateLibraryComplexity.jar', 'CollectGcBiasMetrics.jar']
for sample in samples:
jobname = prefix + '_' + sample
allcmds = []
allcmds.append(cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
paraset = copy.deepcopy(cmdset)
if bam == '=sample':
paraset['INPUT'] = '=%s'%(inputpath+sample)
else:
paraset['INPUT'] = '=%s/%s'%(inputpath+sample, bam)
paraset['TMP_DIR'] = paraset['TMP_DIR'] + prefix + '_' + sample + '/'
cmdGenerator.checkPath(paraset['TMP_DIR'].strip('='), create=createpath)
for metric in metrickeys:
if 'MultipleMetrics' in metric:
paraset['OUTPUT'] = '=%s'%(outputpath + sample + metrics[metric])
else:
paraset['OUTPUT'] = '=%s.txt'%(outputpath + sample + '.' + metrics[metric])
paraset['CHART_OUTPUT'] = '%s'%(paraset['OUTPUT'].replace('.txt', '.pdf'))
paraset['SUMMARY_OUTPUT'] = '%s'%(paraset['OUTPUT'].replace('.txt', '.summary.txt'))
#filter out parameters that are not supported
metricparaset = configRobot.validParas(paraset, availParas[metric])
allcmds.append(cmdGenerator.formatCmd(javacmd, programpath + metric, metricparaset))
allcmds.append(cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)))
allcmds.append(cmdGenerator.formatCmd('rm -Rf', paraset['TMP_DIR'].strip('=')))
jobmanager.createJob(jobname, allcmds, outpath = outputpath, outfn = jobname)
return jobmanager
def GATK_genotyper(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
gatkpath = cmdGenerator.checkPath(cmdset.pop('gatkpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(
cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar' % (int(mem.replace('G', '')) - 2)
GATK = gatkpath + 'GenomeAnalysisTK.jar '
genotyper = '-T UnifiedGenotyper '
CMDs = []
CMDs.append(cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
jobname = prefix
paraset = copy.deepcopy(cmdset)
paraset['-I'] = inputpath + samples[0] + '/' + bam
for si in range(1, len(samples)):
paraset['-I'] = paraset['-I'] + ' -I ' + inputpath + samples[
si] + '/' + bam
CMDs.append(cmdGenerator.formatCmd(javacmd, GATK + genotyper, paraset))
CMDs.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, outputpath)))
jobmanager.createJob(jobname, CMDs, outpath=outputpath, outfn=jobname)
return jobmanager
def RSeQC(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time = configRobot.popParas(cmdset, ['cmd', 'mem', 'time'])
samples, bam, prefix = configRobot.popParas(cmdset,
['sample', 'bam', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
programs = [
'inner_distance.py', 'junction_annotation.py',
'junction_saturation.py', 'read_GC.py', 'read_duplication.py'
]
for sample in samples:
jobname = prefix + '_' + sample
CMDs = []
CMDs.append(
cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
for prog in programs:
paraset = copy.deepcopy(cmdset)
paraset['-i'] = inputpath + sample + '/' + bam
paraset['-o'] = outputpath + sample + '.%s' % (prog.replace(
'.py', ''))
paraset = configRobot.validParas(paraset, availParas[prog])
if '-o' not in paraset.keys():
paraset['>'] = outputpath + sample + '.%s' % (prog.replace(
'.py', ''))
CMDs.append(
cmdGenerator.formatCmd('python', programpath + prog, paraset))
CMDs.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, outputpath)))
jobmanager.createJob(jobname, CMDs, outpath=outputpath, outfn=jobname)
return jobmanager
def picardReorderSam(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(cmdset,['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar'%(int(mem.replace('G',''))-2)
reorder = programpath + 'ReorderSam.jar VALIDATION_STRINGENCY=LENIENT'
for sample in samples:
CMDs = []
CMDs.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
jobname = prefix + '_' + sample
#reorder by chrm
paraset = copy.deepcopy(cmdset)
if bam == '=sample':
inputfile = sample
else:
inputfile = sample + '/' + bam
paraset['INPUT'] = '=%s'%(inputpath + inputfile)
paraset['OUTPUT'] = '=%s.reorder.bam'%(outputpath + inputfile.replace('.bam',''))
paraset = configRobot.validParas(paraset, availParas['ReorderSam.jar'])
CMDs.append( cmdGenerator.formatCmd(javacmd, reorder, paraset) )
CMDs.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMDs, outpath = outputpath, outfn = jobname)
return jobmanager
def markDup(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
javacmd = 'java -Xmx%dg -jar' % (int(mem.replace('G', '')) - 1)
mdupjar = 'MarkDuplicates.jar'
idxcmd = 'samtools index'
for sample in samples:
jobname = prefix + '_' + sample
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s/%s' % (inputpath + sample, bam)
paraset['OUTPUT'] = paraset['INPUT'].replace('.bam', '.mdup.bam')
paraset['METRICS_FILE'] = '=%s/%s' % (inputpath + sample,
prefix + '_mdupmetrics.txt')
paraset = configRobot.validParas(paraset, availParas[mdupjar])
CMDs = []
CMDs.append(
cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
CMDs.append(
cmdGenerator.formatCmd(javacmd, programpath + mdupjar, paraset))
CMDs.append(
cmdGenerator.formatCmd(idxcmd, paraset['OUTPUT'].strip('=')))
CMDs.append(
cmdGenerator.formatCmd(
'mv ./%s%s %s' %
(jobname, jobmanager.ext, inputpath + sample)))
jobmanager.createJob(jobname,
CMDs,
outpath=inputpath + sample,
outfn=jobname)
return jobmanager
def HTSeqCount(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
gtf = configRobot.popParas(cmdset, ['GTF'])
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
for sample in samples:
paraset = copy.deepcopy(cmdset)
jobfnprefix = prefix + '_' + sample
if bam == '=sample':
inputfile = sample
outputfile = sample + '.count'
else:
inputfile = sample + '/' + bam
outputfile = sample + '/' + bam + '.count'
cmdGenerator.checkPath(outputpath + sample, create=createpath)
samcmd = 'samtools view -h %s | ' % (inputpath + inputfile)
htseq = 'python -m HTSeq.scripts.count -q '
countcmd = cmdGenerator.formatCmd(samcmd, htseq, paraset, '-', gtf,
' > %s' % (outputpath + outputfile))
mvscriptcmd = cmdGenerator.formatCmd(
'mv ./%s%s %s' % (jobfnprefix, jobmanager.ext, outputpath))
jobmanager.createJob(jobfnprefix,
[setuppathcmd, countcmd, mvscriptcmd],
outpath=outputpath,
outfn=jobfnprefix)
return jobmanager
def cuffmerge(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, gtf = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'gtf'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
sampletext = '"'
for sample in samples:
sampletext = sampletext + '%s%s/%s\\n'%(inputpath, sample, gtf)
sampletext = sampletext + '"'
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
jobname = prefix
CMD = []
CMD.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outputpath + paraset['-o']
CMD.append( cmdGenerator.formatCmd('echo', sampletext, '>', paraset['-o'] + '.samples') )
paraset = configRobot.validParas(paraset, availParas['cuffmerge'])
cmdGenerator.checkPath(paraset['-o'], create=createpath)
CMD.append( cmdGenerator.formatCmd(cmd, paraset, paraset['-o'] + '.samples') )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, paraset['-o'])) )
CMD.append( cmdGenerator.formatCmd('rm -f', paraset['-o'] + '.samples') )
sgeopt = []
if '-p' in paraset.keys():
if int(paraset['-p']) > 1: #multi threads
sgeopt = ['-pe smp ' + paraset['-p']]
elif '--num-threads' in paraset.keys():
if int(paraset['--num-threads']) > 1:
sgeopt = ['-pe smp ' + paraset['-p']]
jobmanager.createJob(jobname, CMD, outpath=paraset['-o'], outfn=jobname, sgeopt=sgeopt, trackcmd=False)
return jobmanager
def DESeqPair(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmdset = configRobot.makeParasList(cmdset, ['meta', 'group1', 'group2'])
cmd, mem, time, prefix = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'prefix'])
group1, group2 = configRobot.popParas(cmdset, ['group1', 'group2'])
template = open(cmdset.pop('template')).read()
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
cmdset['inputpath'] = inputpath
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
cmdset['outputpath'] = outputpath
cmdset['prefix'] = prefix
meta = configRobot.popParas(cmdset, ['meta'])
if meta[0] == "''" and len(meta) == 1:
cmdset['meta'] = 'c()'
else:
cmdset['meta'] = 'c(\'' + '\', \''.join(meta) + '\')'
if cmdset['countfnprefix'] == "''": cmdset['countfnprefix'] = ''
if cmdset['countfnsuffix'] == "''": cmdset['countfnsuffix'] = ''
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
for i in range(len(group1)):
paraset = copy.deepcopy(cmdset)
paraset['gr1'] = group1[i]
paraset['gr2'] = group2[i]
jobfnprefix = prefix + '_' + group1[i] + '_' + group2[i]
f = open('./%s.R'%(jobfnprefix), 'w')
f.write(template%paraset)
f.close()
deseqcmd = cmdGenerator.formatCmd('Rscript', './%s.R'%(jobfnprefix))
mvRscriptcmd = cmdGenerator.formatCmd('mv ./%s.R %s'%(jobfnprefix, outputpath))
mvscriptcmd = cmdGenerator.formatCmd('mv ./%s%s %s'%(jobfnprefix, jobmanager.ext, outputpath))
jobmanager.createJob(jobfnprefix, [setuppathcmd, deseqcmd, mvRscriptcmd, mvscriptcmd], outpath = outputpath, outfn = jobfnprefix)
return jobmanager
def cuffcompare(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, gtf = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'gtf'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
sampletext = ''
for sample in samples:
sampletext = sampletext + '%s%s/%s ' % (inputpath, sample, gtf)
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
jobname = prefix
CMD = []
CMD.append(cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outputpath + paraset['-o']
paraset = configRobot.validParas(paraset, availParas['cuffcompare'])
CMD.append(cmdGenerator.formatCmd(cmd, paraset, sampletext))
CMD.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, outputpath)))
jobmanager.createJob(jobname,
CMD,
outpath=outputpath,
outfn=jobname,
trackcmd=False)
return jobmanager
def tophat(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix'])
paired = configRobot.popParas(cmdset, ['paired'])
readext, outpath, genome = configRobot.popParas(cmdset, ['readext', 'outputpath', 'genome'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools\necho $BOWTIE2_INDEXES')
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
cmdGenerator.checkPath(outpath + '%s/'%prefix, create=createpath)
for sample in samples:
if paired == 'paired' or paired == 'yes':
reads = map(lambda(i): inputpath + sample + '_%d'%i + readext, [1,2])
elif paired == 'single' or paired == 'no':
reads = inputpath + sample + readext
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outpath + '%s/'%prefix + sample
jobfnprefix = prefix + '_' + sample
tophatcmd = cmdGenerator.formatCmd(cmd, paraset, genome, reads)
mvscriptcmd = cmdGenerator.formatCmd('mv ./%s%s %s'%(jobfnprefix, jobmanager.ext, paraset['-o']))
if int(paraset['-p']) > 1: #multiple threads per job
sgeopt = ['-pe smp ' + paraset['-p']]
else:
sgeopt = []
#need to create the output directory first, otherwise SGE complains cannot put the stdout in its path
cmdGenerator.checkPath(paraset['-o'], create=createpath)
jobmanager.createJob(jobfnprefix, [setuppathcmd, tophatcmd, mvscriptcmd], outpath = paraset['-o'], outfn = jobfnprefix, sgeopt=sgeopt)
return jobmanager
def GATK_genotyper(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(cmdset,['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
gatkpath = cmdGenerator.checkPath(cmdset.pop('gatkpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar'%(int(mem.replace('G',''))-2)
GATK = gatkpath + 'GenomeAnalysisTK.jar '
genotyper = '-T UnifiedGenotyper '
CMDs = []
CMDs.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
jobname = prefix
paraset = copy.deepcopy(cmdset)
paraset['-I'] = inputpath + samples[0] + '/' + bam
for si in range(1,len(samples)):
paraset['-I'] = paraset['-I'] + ' -I ' + inputpath + samples[si] + '/' + bam
CMDs.append( cmdGenerator.formatCmd(javacmd, GATK+genotyper, paraset) )
CMDs.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMDs, outpath = outputpath, outfn = jobname)
return jobmanager
def RNASeQC(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time = configRobot.popParas(cmdset, ['cmd', 'mem', 'time'])
samples, bam, prefix = configRobot.popParas(cmdset, ['sample', 'bam', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar'%(int(mem.replace('G',''))-2)
#need to generate -s, -o
#generate a temperory file
samplestr = '"Sample ID\\tBam File\\tNotes\\n'
for sample in samples:
samplestr = samplestr + '%s\\t%s\\t%s\\n'%(sample, inputpath + sample + '/' + bam, sample)
samplestr = samplestr + '"'
samplefile = '%s.samples'%prefix
cmdset['-s'] = samplefile
cmdset['-o'] = inputpath + 'RNA-SeQC/'
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
setupcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
createsamplefile = cmdGenerator.formatCmd('echo', samplestr, '>', samplefile)
removesamplefile = cmdGenerator.formatCmd('rm -f', samplefile)
mvscriptcmd = cmdGenerator.formatCmd('mv %s%s %s'%(prefix, jobmanager.ext, cmdset['-o']))
qccmd = cmdGenerator.formatCmd(javacmd, programpath+cmd, cmdset)
cmdGenerator.checkPath(cmdset['-o'], create=createpath)
jobmanager.createJob(prefix, [setupcmd, createsamplefile, qccmd, removesamplefile, mvscriptcmd], outfn = prefix, outpath = cmdset['-o'], trackcmd=False)
return jobmanager
def runBootstrap(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, call, mem, time, prefix, datasets, phenos, njobs = configRobot.popParas(cmdset, ['cmd', 'call', 'mem', 'time', 'prefix', 'dataset', 'pheno', 'njob'])
iter, usecn, combineSplit, useDisease = configRobot.popParas(cmdset, ['iter', 'usecn', 'combineSplit', 'useDisease'])
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
cmdGenerator.checkPath(outputpath, create=createpath)
if type(datasets) != type([]): datasets = [datasets]
if type(njobs) != type([]): njobs = [njobs]
if type(usecn) != type([]): usecn = [usecn]
if type(combineSplit) != type(combineSplit): combineSplit = [combineSplit]
for di in range(len(datasets)):
numjobs = int(njobs[di])
for cnSet in usecn:
if cnSet == 'true': cnStr = 'C1'
else: cnStr = 'C0'
for splitSet in combineSplit:
if splitSet == 'true': splitStr = 'S1'
else: splitStr = 'S0'
for jobidx in range(1, numjobs+1):
CMD = []
fnhead = prefix + datasets[di] + cnStr + splitStr
jobname = prefix + datasets[di] + cnStr + splitStr + 'J%02d'%(jobidx)
for iteridx in range(1, int(iter)+1):
functionCall = "addpath(\'./Gray/data\'); tic; " + call + "(%d, %d, %d, '%s', '%s', 'usecn', %s, 'combineSplit', %s, 'useDisease', %s); toc;"%(jobidx, numjobs, iteridx, outputpath, fnhead, cnSet, splitSet, useDisease )
CMD.append( cmdGenerator.formatCmd( matlabcmd%(functionCall) ) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def countmismatches(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
direct = ['forward', 'reverse']
for sample in samples:
for d in direct:
jobname = prefix + '_' + sample + '_' + d
CMD = []
CMD.append(
cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
CMD.append(
cmdGenerator.formatCmd(
'python', 'countmismatches.py',
inputpath + sample + '/' + bam,
outputpath + sample + '.%s.countmis' % d, d))
CMD.append(
cmdGenerator.formatCmd('mv',
'%s%s' % (jobname, jobmanager.ext),
outputpath))
jobmanager.createJob(jobname,
CMD,
outpath=outputpath,
outfn=jobname,
trackcmd=False)
return jobmanager
def countmismatches(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(cmdset,['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
direct = ['forward', 'reverse']
for sample in samples:
for d in direct:
jobname = prefix+'_'+sample+'_'+d
CMD = []
CMD.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
CMD.append( cmdGenerator.formatCmd('python','countmismatches.py',inputpath+sample+'/'+bam, outputpath+sample+'.%s.countmis'%d, d) )
CMD.append( cmdGenerator.formatCmd('mv', '%s%s'%(jobname, jobmanager.ext), outputpath) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def cuffdiff_v1(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, gtf, bam = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'gtf', 'bam'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
sampletext = ''
for sample in samples:
sampletext = sampletext + '%s%s/%s '%(inputpath, sample, bam)
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
jobname = prefix
CMD = []
CMD.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
paraset = copy.deepcopy(cmdset)
paraset = configRobot.validParas(paraset, availParas['cuffdiff'])
cmdGenerator.checkPath(paraset['--output-dir'], create=createpath)
CMD.append( cmdGenerator.formatCmd('/ifs/home/c2b2/dp_lab/bc2252/SeqTool/cufflinks_1/cuffdiff', paraset, gtf, sampletext) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, paraset['--output-dir'])) )
sgeopt = []
if '-p' in paraset.keys():
if int(paraset['-p']) > 1: #multi threads
sgeopt = ['-pe smp ' + paraset['-p']]
elif '--num-threads' in paraset.keys():
if int(paraset['--num-threads']) > 1:
sgeopt = ['-pe smp ' + paraset['-p']]
jobmanager.createJob(jobname, CMD, outpath=paraset['--output-dir'], outfn=jobname, sgeopt=sgeopt, trackcmd=False)
return jobmanager
def cufflinks(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'), create=createpath)
outputpath = cmdGenerator.checkPath(outputpath + '%s/'%prefix, create=createpath)
if type(samples) != type([]) and type(samples) != type(()):
samples = [samples]
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
for sample in samples:
jobname = prefix + '_' + sample
CMD = []
CMD.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
paraset = copy.deepcopy(cmdset)
paraset['-o'] = outputpath + sample
paraset = configRobot.validParas(paraset, availParas['cufflinks'])
cmdGenerator.checkPath(paraset['-o'], create=createpath)
CMD.append( cmdGenerator.formatCmd(cmd, paraset, inputpath+'%s/'%sample+bam) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, paraset['-o'])) )
sgeopt = []
if '-p' in paraset.keys():
if int(paraset['-p']) > 1: #multi threads
sgeopt = ['-pe smp ' + paraset['-p']]
elif '--num-threads' in paraset.keys():
if int(paraset['--num-threads']) > 1:
sgeopt = ['-pe smp ' + paraset['-p']]
jobmanager.createJob(jobname, CMD, outpath=paraset['-o'], outfn=jobname, sgeopt=sgeopt)
return jobmanager
def runBootstrapCCLE(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmdset = configRobot.makeParasList(cmdset, ['njob', 'dataset', 'pheno', 'usecn', 'useDisease', 'combineSplit', 'priormethod', 'ylogtransform', 'useallexp', 'cumulatecount', 'predList'])
cmd, call, mem, time, prefix, njobs = configRobot.popParas(cmdset, ['cmd', 'call', 'mem', 'time', 'prefix', 'njob'])
iter, priormethod, algo, ylogtransform = configRobot.popParas(cmdset, ['iter', 'priormethod', 'algo', 'ylogtransform'])
datasets, phenos, usecn, combineSplit, useDisease = configRobot.popParas(cmdset, ['dataset', 'pheno', 'usecn', 'combineSplit', 'useDisease'])
useallexp = configRobot.popParas(cmdset, ['useallexp'])
if 'trainmode' in cmdset.keys():
trainmode = configRobot.popParas(cmdset, ['trainmode'])
else: trainmode = 'false'
if 'cumulatecount' in cmdset.keys():
cumulatecount = configRobot.popParas(cmdset, ['cumulatecount'])
if 'runcv' in cmdset.keys():
runcv = range(1, int(configRobot.popParas(cmdset, ['runcv']))+1)
else: runcv = [0]
if 'samplinghead' in cmdset.keys():
sampling = configRobot.popParas(cmdset, ['samplinghead'])
else: sampling = 'Samplings'
if 'lassoparahead' in cmdset.keys():
lassopara = configRobot.popParas(cmdset, ['lassoparahead'])
else: lassopara = 'lassCvPara'
if 'predList' in cmdset.keys():
predlist = configRobot.popParas(cmdset, ['predList'])
else:
predlist = ['cancerGenes']
iterlist = list(itertools.product( datasets, phenos, usecn, combineSplit, useDisease, priormethod, ylogtransform, useallexp, cumulatecount, runcv, predlist ) )
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
cmdGenerator.checkPath(outputpath, create=createpath)
f = popen('ls %s*.finished'%(outputpath))
finfns = f.read().split()
f.close()
finfns = map(lambda(l):l.replace(outputpath, '').replace('.finished', ''), finfns)
for dataset, pheno, cnSet, splitSet, diseaseSet, prior, ylog, useExp, cumcount, cvidx, plist in iterlist:
if pheno in ['ACT', 'GI50'] and ylog == 'true': continue
if len(njobs) == 1:
numjobs = int(njobs[0])
else:
numjobs = int(njobs[ datasets.index(dataset) ] )
if cnSet == 'true': cnStr = 'C1'
else: cnStr = 'C0'
if splitSet == 'true': splitStr = 'S1'
else: splitStr = 'S0'
if diseaseSet == 'true': diseaseStr = 'D1'
else: diseaseStr = 'D0'
if prior == 'bootstrapnorm': priorStr = 'NRM'
else: priorStr = ''
if ylog == 'true': logStr = 'log'
else: logStr = ''
if useExp == 'true': expStr = 'AE'
else: expStr = ''
if cumcount == 'true': cumStr = 'Cum'
else: cumStr = ''
if cvidx == 0: cvStr = ''
else: cvStr = '%02d'%(cvidx)
if plist == 'cancerGenes': plStr = ''
else: plStr = plist
if pheno == '[]': pheno = ''
for jobidx in range(1, numjobs+1):
fnhead = prefix + cvStr + plStr + priorStr + logStr + cnStr + splitStr + diseaseStr + expStr + cumStr
jobname = prefix + cvStr + plStr + priorStr + logStr + dataset + pheno + cnStr + splitStr + diseaseStr + expStr + cumStr + 'J%02d'%(jobidx)
CMD = []
for iteridx in range(1, int(iter)+1):
if fnhead+dataset+'_J%02dI%02d'%(jobidx,iteridx) in finfns: continue
if dataset.lower() == 'joe':
functionCall = "addpath(\'./Gray/data\'); tic; "
elif 'ccle' in dataset.lower():
functionCall = "addpath(\'./CCLE/data\'); tic; "
functionCall = functionCall + call + "(%d, %d, %d, '%s', '%s', '%s', '%s', 'usecn', %s, 'combineSplit', %s, 'useDisease', %s, 'algo', '%s', 'priormethod', '%s', 'ylogtransform', %s, 'trainmode', %s, 'useallexp', %s, 'cumulatecount', %s, 'sampling', '%s', 'lassopara', '%s', 'predList', '%s'); toc;"%(jobidx, numjobs, iteridx, outputpath, fnhead, dataset, pheno, cnSet, splitSet, diseaseSet, algo, prior, ylog, trainmode, useExp, cumcount, sampling+cvStr, lassopara+cvStr, plist )
if algo == 'lasso':
CMD.append( cmdGenerator.formatCmd( matlabcmd2012%(functionCall) ) )
else:
CMD.append( cmdGenerator.formatCmd( matlabcmd%(functionCall) ) )
if len(CMD) > 0:
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def picardQC(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, bam, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'bam', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'),
create=createpath)
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
samples = cmdset.pop('sample')
javacmd = 'java -Xmx%dg -jar' % (int(mem.replace('G', '')) - 2)
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
metrics = {
'CollectRnaSeqMetrics.jar': 'RnaSeq',
'CollectMultipleMetrics.jar': '',
'EstimateLibraryComplexity.jar': 'Lib',
'CollectGcBiasMetrics.jar': 'GC'
}
metrickeys = [
'CollectRnaSeqMetrics.jar', 'CollectMultipleMetrics.jar',
'EstimateLibraryComplexity.jar', 'CollectGcBiasMetrics.jar'
]
for sample in samples:
jobname = prefix + '_' + sample
allcmds = []
allcmds.append(
cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
paraset = copy.deepcopy(cmdset)
if bam == '=sample':
paraset['INPUT'] = '=%s' % (inputpath + sample)
else:
paraset['INPUT'] = '=%s/%s' % (inputpath + sample, bam)
paraset['TMP_DIR'] = paraset['TMP_DIR'] + prefix + '_' + sample + '/'
cmdGenerator.checkPath(paraset['TMP_DIR'].strip('='),
create=createpath)
for metric in metrickeys:
if 'MultipleMetrics' in metric:
paraset['OUTPUT'] = '=%s' % (outputpath + sample +
metrics[metric])
else:
paraset['OUTPUT'] = '=%s.txt' % (outputpath + sample + '.' +
metrics[metric])
paraset['CHART_OUTPUT'] = '%s' % (paraset['OUTPUT'].replace(
'.txt', '.pdf'))
paraset['SUMMARY_OUTPUT'] = '%s' % (paraset['OUTPUT'].replace(
'.txt', '.summary.txt'))
#filter out parameters that are not supported
metricparaset = configRobot.validParas(paraset, availParas[metric])
allcmds.append(
cmdGenerator.formatCmd(javacmd, programpath + metric,
metricparaset))
allcmds.append(
cmdGenerator.formatCmd('mv ./%s%s %s' %
(jobname, jobmanager.ext, outputpath)))
allcmds.append(
cmdGenerator.formatCmd('rm -Rf', paraset['TMP_DIR'].strip('=')))
jobmanager.createJob(jobname,
allcmds,
outpath=outputpath,
outfn=jobname)
return jobmanager
def runCV(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmdset = configRobot.makeParasList(cmdset, ['njob', 'dataset', 'pheno', 'usecn', 'useDisease', 'combineSplit', 'ylogtransform'])
cmd, call, mem, time, prefix, njobs = configRobot.popParas(cmdset, ['cmd', 'call', 'mem', 'time', 'prefix', 'njob'])
datasets, phenos, usecn, useDisease, combineSplit = configRobot.popParas(cmdset, ['dataset', 'pheno', 'usecn', 'useDisease', 'combineSplit'])
ylogtransform, trainmode = configRobot.popParas(cmdset, ['ylogtransform', 'trainmode'])
if 'useallexp' in cmdset.keys():
useallexp = configRobot.popParas(cmdset, ['useallexp'])
else: useallexp = 'false'
if 'samplinghead' in cmdset.keys():
samplinghead = configRobot.popParas(cmdset, ['samplinghead'])
else: samplinghead = 'Samplings'
if 'runcv' in cmdset.keys():
runcv = range(1, int(configRobot.popParas(cmdset, ['runcv']))+1)
else: runcv = [0]
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
cmdGenerator.checkPath(outputpath, create=createpath)
iterlist = list(itertools.product( datasets, phenos, usecn, combineSplit, useDisease, ylogtransform, runcv ) )
for dataset, pheno, cnSet, splitSet, diseaseSet, ylog, cvidx in iterlist:
if pheno in ['ACT', 'GI50'] and ylog == 'true': continue
if len(njobs) == 1:
numjobs = int(njobs[0])
else:
numjobs = int(njobs[ datasets.index(dataset) ] )
if cnSet == 'true': cnStr = 'C1'
else: cnStr = 'C0'
if splitSet == 'true': splitStr = 'S1'
else: splitStr = 'S0'
if diseaseSet == 'true': diseaseStr = 'D1'
else: diseaseStr = 'D0'
if ylog == 'true': logStr = 'log'
else: logStr = ''
if cvidx == 0: cvStr = ''
else: cvStr = '%02d'%(cvidx)
if pheno == '[]': pheno = ''
for jobidx in range(1, numjobs+1):
fnhead = prefix + cvStr + logStr + cnStr + splitStr + diseaseStr
jobname = prefix + cvStr + logStr + dataset + pheno + cnStr + splitStr + diseaseStr + 'J%02d'%(jobidx)
CMD = []
if dataset.lower() == 'joe':
functionCall = "addpath(\'./Gray/data\'); tic; "
else:
functionCall = "addpath(\'./CCLE/data\'); tic; "
functionCall = functionCall + call + "(%d, %d, '%s', '%s', '%s', '%s', 'usecn', %s, 'combineSplit', %s, 'useDisease', %s, 'ylogtransform', %s, 'trainmode', %s, 'sampling', '%s'); toc;"%(jobidx, numjobs, outputpath, fnhead, dataset, pheno, cnSet, splitSet, diseaseSet, ylog, trainmode, samplinghead+cvStr )
CMD.append( cmdGenerator.formatCmd( matlabcmd2012%(functionCall) ) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
def preGATK(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(cmdset,['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
picardpath = cmdGenerator.checkPath(cmdset.pop('picardpath'))
gatkpath = cmdGenerator.checkPath(cmdset.pop('gatkpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar'%(int(mem.replace('G',''))-2)
samview = 'samtools view -b -h -F 264'
reorder = picardpath + 'ReorderSam.jar VALIDATION_STRINGENCY=LENIENT'
RG = picardpath + 'AddOrReplaceReadGroups.jar VALIDATION_STRINGENCY=LENIENT RGLB=dUTP RGPL=illumina RGPU=1'
mdupjar = picardpath + 'MarkDuplicates.jar'
GATK = gatkpath + 'GenomeAnalysisTK.jar '
createTg = '-T RealignerTargetCreator '
realign = '-T IndelRealigner '
idxcmd = 'samtools index'
clearup = 'rm -f '
for sample in samples:
CMDs = []
CMDs.append( cmdGenerator.formatCmd('source ~/libraries/setup_seqtools') )
jobname = prefix + '_' + sample
#filter
paraset = copy.deepcopy(cmdset)
paraset['-o'] = '%s/%s.filter.bam'%(inputpath+sample, bam.replace('.bam',''))
lastoutput = paraset['-o']
del paraset['-R']
del paraset['-filterMBQ']
#paraset = configRobot.validParas(paraset, availParas['samtools'])
CMDs.append( cmdGenerator.formatCmd(samview, paraset, inputpath+sample+'/'+bam ) )
#reorder by chrm
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s'%lastoutput
paraset['OUTPUT'] = '=%s.reorder.bam'%(lastoutput.replace('.bam',''))
paraset['REFERENCE'] = '=%s'%paraset['-R']
paraset = configRobot.validParas(paraset, availParas['ReorderSam.jar'])
CMDs.append( cmdGenerator.formatCmd(javacmd, reorder, paraset) )
CMDs.append( cmdGenerator.formatCmd(clearup, lastoutput) )
lastoutput = paraset['OUTPUT'].strip('=')
#add RG
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s'%lastoutput
paraset['OUTPUT'] = '=%s.addRG.bam'%(lastoutput.replace('.bam',''))
paraset['RGSM'] = '=%s'%sample
paraset = configRobot.validParas(paraset, availParas['AddOrReplaceReadGroups.jar'])
CMDs.append( cmdGenerator.formatCmd(javacmd, RG, paraset) )
CMDs.append( cmdGenerator.formatCmd(clearup, lastoutput) )
lastoutput = paraset['OUTPUT'].strip('=')
#mark duplicates
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s'%lastoutput
paraset['OUTPUT'] = '=%s.mdup.bam'%(lastoutput.replace('.bam', ''))
paraset['METRICS_FILE'] = '=%s/%s'%(inputpath + sample, prefix + '_mdupmetrics.txt')
paraset = configRobot.validParas(paraset, availParas['MarkDuplicates.jar'])
CMDs.append( cmdGenerator.formatCmd(javacmd, mdupjar, paraset) )
CMDs.append( cmdGenerator.formatCmd(idxcmd, paraset['OUTPUT'].strip('=')) )
lastoutput = paraset['OUTPUT'].strip('=')
#create intervals
paraset = copy.deepcopy(cmdset)
paraset['-I'] = lastoutput
paraset['-o'] = lastoutput.replace('.bam', '.intervals')
CMDs.append( cmdGenerator.formatCmd(javacmd, GATK+createTg, paraset) )
#realign
paraset['-targetIntervals'] = paraset['-o']
paraset['-o'] = lastoutput.replace('.bam', '.realign.bam')
CMDs.append( cmdGenerator.formatCmd(javacmd, GATK+realign, paraset) )
#clear up
CMDs.append( cmdGenerator.formatCmd(clearup, lastoutput) )
CMDs.append( cmdGenerator.formatCmd(clearup, lastoutput.replace('.bam', '.intervals')) )
CMDs.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, inputpath+sample)) )
jobmanager.createJob(jobname, CMDs, outpath = inputpath+sample, outfn = jobname)
return jobmanager
def preGATK(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix'])
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
picardpath = cmdGenerator.checkPath(cmdset.pop('picardpath'))
gatkpath = cmdGenerator.checkPath(cmdset.pop('gatkpath'))
bam = configRobot.popParas(cmdset, 'bam')
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
if '-Djava.io.tmpdir' in cmdset.keys():
javacmd = 'java ' + '-Djava.io.tmpdir' + cmdGenerator.checkPath(
cmdset.pop('-Djava.io.tmpdir'))
else:
javacmd = 'java'
javacmd = javacmd + ' -Xmx%dg -jar' % (int(mem.replace('G', '')) - 2)
samview = 'samtools view -b -h -F 264'
reorder = picardpath + 'ReorderSam.jar VALIDATION_STRINGENCY=LENIENT'
RG = picardpath + 'AddOrReplaceReadGroups.jar VALIDATION_STRINGENCY=LENIENT RGLB=dUTP RGPL=illumina RGPU=1'
mdupjar = picardpath + 'MarkDuplicates.jar'
GATK = gatkpath + 'GenomeAnalysisTK.jar '
createTg = '-T RealignerTargetCreator '
realign = '-T IndelRealigner '
idxcmd = 'samtools index'
clearup = 'rm -f '
for sample in samples:
CMDs = []
CMDs.append(
cmdGenerator.formatCmd('source ~/libraries/setup_seqtools'))
jobname = prefix + '_' + sample
#filter
paraset = copy.deepcopy(cmdset)
paraset['-o'] = '%s/%s.filter.bam' % (inputpath + sample,
bam.replace('.bam', ''))
lastoutput = paraset['-o']
del paraset['-R']
del paraset['-filterMBQ']
#paraset = configRobot.validParas(paraset, availParas['samtools'])
CMDs.append(
cmdGenerator.formatCmd(samview, paraset,
inputpath + sample + '/' + bam))
#reorder by chrm
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s' % lastoutput
paraset['OUTPUT'] = '=%s.reorder.bam' % (lastoutput.replace(
'.bam', ''))
paraset['REFERENCE'] = '=%s' % paraset['-R']
paraset = configRobot.validParas(paraset, availParas['ReorderSam.jar'])
CMDs.append(cmdGenerator.formatCmd(javacmd, reorder, paraset))
CMDs.append(cmdGenerator.formatCmd(clearup, lastoutput))
lastoutput = paraset['OUTPUT'].strip('=')
#add RG
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s' % lastoutput
paraset['OUTPUT'] = '=%s.addRG.bam' % (lastoutput.replace('.bam', ''))
paraset['RGSM'] = '=%s' % sample
paraset = configRobot.validParas(
paraset, availParas['AddOrReplaceReadGroups.jar'])
CMDs.append(cmdGenerator.formatCmd(javacmd, RG, paraset))
CMDs.append(cmdGenerator.formatCmd(clearup, lastoutput))
lastoutput = paraset['OUTPUT'].strip('=')
#mark duplicates
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s' % lastoutput
paraset['OUTPUT'] = '=%s.mdup.bam' % (lastoutput.replace('.bam', ''))
paraset['METRICS_FILE'] = '=%s/%s' % (inputpath + sample,
prefix + '_mdupmetrics.txt')
paraset = configRobot.validParas(paraset,
availParas['MarkDuplicates.jar'])
CMDs.append(cmdGenerator.formatCmd(javacmd, mdupjar, paraset))
CMDs.append(
cmdGenerator.formatCmd(idxcmd, paraset['OUTPUT'].strip('=')))
lastoutput = paraset['OUTPUT'].strip('=')
#create intervals
paraset = copy.deepcopy(cmdset)
paraset['-I'] = lastoutput
paraset['-o'] = lastoutput.replace('.bam', '.intervals')
CMDs.append(cmdGenerator.formatCmd(javacmd, GATK + createTg, paraset))
#realign
paraset['-targetIntervals'] = paraset['-o']
paraset['-o'] = lastoutput.replace('.bam', '.realign.bam')
CMDs.append(cmdGenerator.formatCmd(javacmd, GATK + realign, paraset))
#clear up
CMDs.append(cmdGenerator.formatCmd(clearup, lastoutput))
CMDs.append(
cmdGenerator.formatCmd(clearup,
lastoutput.replace('.bam', '.intervals')))
CMDs.append(
cmdGenerator.formatCmd(
'mv ./%s%s %s' %
(jobname, jobmanager.ext, inputpath + sample)))
jobmanager.createJob(jobname,
CMDs,
outpath=inputpath + sample,
outfn=jobname)
return jobmanager
def filetersingleton(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(cmdset,['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
if 'TMP_DIR' in cmdset.keys():
TMP_DIR = cmdset.pop('TMP_DIR')
else:
TMP_DIR = ''
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
javacmd = 'java -Xmx%dg -jar'%(int(mem.replace('G',''))-1)
samview = 'samtools view -b -h -F 8'
reorder = 'ReorderSam.jar VALIDATION_STRINGENCY=LENIENT'
RG = 'AddOrReplaceReadGroups.jar VALIDATION_STRINGENCY=LENIENT RGLB=dUTP RGPL=illumina RGPU=1'
mdup = 'MarkDuplicates.jar'
for sample in samples:
jobfnprefix = prefix + '_' + sample
paraset = copy.deepcopy(cmdset)
if TMP_DIR != '': paraset['TMP_DIR'] = '%s'%TMP_DIR
tmpbam = []
CMDs = []
CMDs.append(setuppathcmd)
paraset['INPUT'] = '=%s/%s'%(inputpath+sample, bam)
paraset['OUTPUT'] = '=%s/%s.reorder.bam'%(inputpath+sample, bam.replace('.bam',''))
tmpbam.append(paraset['OUTPUT'].strip('='))
CMDs.append( cmdGenerator.formatCmd(javacmd, programpath+reorder, paraset) )
paraset['INPUT'] = '=%s/%s.reorder.bam'%(inputpath+sample, bam.replace('.bam',''))
paraset['OUTPUT'] = '=%s/%s.reorder.addRG.bam'%(inputpath+sample, bam.replace('.bam',''))
paraset['RGSM'] = '=%s'%sample
CMDs.append( cmdGenerator.formatCmd(javacmd, programpath+RG, paraset) )
paraset = copy.deepcopy(cmdset)
paraset['-o'] = '%s/%s.filter.bam'%(inputpath+sample, bam.replace('.bam','.addRG'))
CMDs.append( cmdGenerator.formatCmd(samview, paraset, inputpath+sample+'/'+bam.replace('.bam','.addRG.bam')) )
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s/%s.filter.bam'%(inputpath+sample, bam.replace('.bam','.addRG'))
paraset['OUTPUT'] = '%s/%s.mdup.bam'%(paraset['INPUT'].replace('.bam', ''))
CMDs.append( cmdGenerator.formatCmd(javacmd, programpath+mdup, paraset) )
paraset = copy.deepcopy(cmdset)
CMDs.append( cmdGenerator.formatCmd('samtools index', bam.replace('.bam', '.reorder.addRG.filter.mdup.bam')) )
CMDs.append( cmdGenerator.formatCmd('rm -f', tmpbam) )
CMDs.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobfnprefix, jobmanager.ext, inputpath+sample)) )
jobmanager.createJob(jobfnprefix, CMDs, outpath = inputpath+sample, outfn = jobfnprefix)
return jobmanager
def filetersingleton(cmdset, runmode='test'):
global availParas
if runmode == 'test':
createpath = False
else:
createpath = True
cmd, mem, time, samples, prefix, bam = configRobot.popParas(
cmdset, ['cmd', 'mem', 'time', 'sample', 'prefix', 'bam'])
if 'TMP_DIR' in cmdset.keys():
TMP_DIR = cmdset.pop('TMP_DIR')
else:
TMP_DIR = ''
inputpath = cmdGenerator.checkPath(cmdset.pop('inputpath'))
programpath = cmdGenerator.checkPath(cmdset.pop('programpath'))
jobmanager = jobFactory.jobManager(mem=mem,
time=time,
overwrite=cmdset.pop('overwrite'))
setuppathcmd = cmdGenerator.formatCmd('source ~/libraries/setup_seqtools')
javacmd = 'java -Xmx%dg -jar' % (int(mem.replace('G', '')) - 1)
samview = 'samtools view -b -h -F 8'
reorder = 'ReorderSam.jar VALIDATION_STRINGENCY=LENIENT'
RG = 'AddOrReplaceReadGroups.jar VALIDATION_STRINGENCY=LENIENT RGLB=dUTP RGPL=illumina RGPU=1'
mdup = 'MarkDuplicates.jar'
for sample in samples:
jobfnprefix = prefix + '_' + sample
paraset = copy.deepcopy(cmdset)
if TMP_DIR != '': paraset['TMP_DIR'] = '%s' % TMP_DIR
tmpbam = []
CMDs = []
CMDs.append(setuppathcmd)
paraset['INPUT'] = '=%s/%s' % (inputpath + sample, bam)
paraset['OUTPUT'] = '=%s/%s.reorder.bam' % (inputpath + sample,
bam.replace('.bam', ''))
tmpbam.append(paraset['OUTPUT'].strip('='))
CMDs.append(
cmdGenerator.formatCmd(javacmd, programpath + reorder, paraset))
paraset['INPUT'] = '=%s/%s.reorder.bam' % (inputpath + sample,
bam.replace('.bam', ''))
paraset['OUTPUT'] = '=%s/%s.reorder.addRG.bam' % (
inputpath + sample, bam.replace('.bam', ''))
paraset['RGSM'] = '=%s' % sample
CMDs.append(cmdGenerator.formatCmd(javacmd, programpath + RG, paraset))
paraset = copy.deepcopy(cmdset)
paraset['-o'] = '%s/%s.filter.bam' % (inputpath + sample,
bam.replace('.bam', '.addRG'))
CMDs.append(
cmdGenerator.formatCmd(
samview, paraset,
inputpath + sample + '/' + bam.replace('.bam', '.addRG.bam')))
paraset = copy.deepcopy(cmdset)
paraset['INPUT'] = '=%s/%s.filter.bam' % (
inputpath + sample, bam.replace('.bam', '.addRG'))
paraset['OUTPUT'] = '%s/%s.mdup.bam' % (paraset['INPUT'].replace(
'.bam', ''))
CMDs.append(
cmdGenerator.formatCmd(javacmd, programpath + mdup, paraset))
paraset = copy.deepcopy(cmdset)
CMDs.append(
cmdGenerator.formatCmd(
'samtools index',
bam.replace('.bam', '.reorder.addRG.filter.mdup.bam')))
CMDs.append(cmdGenerator.formatCmd('rm -f', tmpbam))
CMDs.append(
cmdGenerator.formatCmd(
'mv ./%s%s %s' %
(jobfnprefix, jobmanager.ext, inputpath + sample)))
jobmanager.createJob(jobfnprefix,
CMDs,
outpath=inputpath + sample,
outfn=jobfnprefix)
return jobmanager
def testSim(cmdset, runmode='test'):
if runmode == 'test':
createpath = False
else:
createpath = True
cmdset = configRobot.makeParasList(cmdset, ['njob', 'dataset', 'cumulatecount', 'costCoef', 'priormethod', 'transresidual', 'btthres', 'adaptiveSigmoid', 'splitpriornorm'])
cmd, call, mem, time, prefix, njobs = configRobot.popParas(cmdset, ['cmd', 'call', 'mem', 'time', 'prefix', 'njob'])
datasets, algo, noiseidx, cumulatecount, costCoef, priormethod, transresidual, btthres, adaptiveSigmoid, splitpriornorm = configRobot.popParas(cmdset, ['dataset', 'algo', 'noiseidx', 'cumulatecount', 'costCoef', 'priormethod','transresidual', 'btthres', 'adaptiveSigmoid', 'splitpriornorm'])
outputpath = cmdGenerator.checkPath(cmdset.pop('outputpath'))
jobmanager = jobFactory.jobManager(mem=mem, time=time, overwrite=cmdset.pop('overwrite'))
cmdGenerator.checkPath(outputpath, create=createpath)
iterlist = list(itertools.product( datasets, cumulatecount, noiseidx, costCoef, priormethod, transresidual, btthres, adaptiveSigmoid, splitpriornorm ) )
for dataset, cumcount, nsidx, betacost, prmethod, transres, btthreshold, adaptSig, splitNorm in iterlist:
if len(njobs) == 1:
numjobs = int(njobs[0])
else:
numjobs = int(njobs[ datasets.index(dataset) ] )
if cumcount == 'true': cumStr = 'Cum1'
else: cumStr = 'Cum0'
if prmethod == 'bootstrap': prStr = 'BT'
elif prmethod == 'bootstraplinear': prStr = 'BL'
elif prmethod == 'bootstrapexpected': prStr = 'BE'
elif prmethod == 'bayes': prStr = 'BY'
else:
print 'unknown prior method:', prmethod
exit(1)
if transres == 'true':
if prmethod != 'bootstrap': continue
else: resStr = 'Resd1' + '_' + btthreshold
else:
resStr = 'Resd0'
if btthreshold != '0.3': continue
else: resStr = resStr + '_0.3'
if adaptSig == 'true': sigStr = 'ADSig1'
else: sigStr = 'ADSig0'
if splitNorm == 'true': splitnormStr = 'SPN1'
else: splitnormStr = 'SPN0'
if splitNorm == 'true' and prmethod != 'bootstrapexpected': continue
resStr = resStr.replace('.','')
Nstr = 'N%s'%(nsidx)
#costStr = 'P' + betacost
costStr = ''
cumStr = ''
for jobidx in range(1, numjobs+1):
fnhead = prefix + dataset + cumStr + costStr + Nstr + prStr + resStr + sigStr + splitnormStr
jobname = prefix + dataset + cumStr + costStr + Nstr + prStr + resStr + sigStr + splitnormStr + 'J%02d'%(jobidx)
CMD = []
functionCall = "tic; "
functionCall = functionCall + call + "(%d, %d, '%s', '%s', %s, '%s', '%s', 'cumulatecount', %s, 'costCoef', %s, 'priormethod', '%s', 'transresidual', %s, 'adaptiveSigmoid', %s, 'btthres', %s, 'splitpriornorm', %s); toc;"%(jobidx, numjobs, dataset, algo, nsidx, outputpath, fnhead, cumcount, betacost, prmethod, transres, adaptSig, btthreshold, splitNorm)
if algo == 'lasso':
CMD.append( cmdGenerator.formatCmd( matlabcmd2012%(functionCall) ) )
else:
CMD.append( cmdGenerator.formatCmd( matlabcmd%(functionCall) ) )
CMD.append( cmdGenerator.formatCmd('mv ./%s%s %s'%(jobname, jobmanager.ext, outputpath)) )
jobmanager.createJob(jobname, CMD, outpath=outputpath, outfn=jobname, trackcmd=False)
return jobmanager
