def calc_tree(alignment):
a = egglib.Align(alignment)
a = a.extract(0, len(a.sequence(1)) - 3) # remove stop codon
if len(a.sequence(1))%3 != 0:
print(alignment, " not in frame")
return (a, None)
if a.ns() < 3:
return (a, None)
for i in range(a.ns()):
a.sequence(i, sequence=a.sequence(i).upper())
tree, loglk = wrappers.phyml(a)
return (a, tree)
def withinGeneLD(directory):
outfile = open("LDstats.txt", "w")
outfile.write("Gene\tPosition1\tPosition2\tDistance\tD'\tR2\n")
for f in listdir_fullpath(directory):
a = egglib.Align(f)
gene = os.path.basename(f).split("_")[0]
siteIndices = a.polymorphism()['siteIndices']
ldStats = a.matrixLD()
for i in range(len(siteIndices)):
for j in range(i + 1, len(siteIndices)):
pos1 = siteIndices[i]
pos2 = siteIndices[j]
distance = ldStats['d'][pos1][pos2]
dPrime = ldStats['Dp'][pos1][pos2]
rSquared = ldStats['r2'][pos1][pos2]
outfile.write("{0}\t{1}\t{2}\t{3}\t{4}\t{5}\n".format(
gene, pos1, pos2, distance, dPrime, rSquared))
outfile.close()
def get_seqid_str(ref_genome, chrom):
""" Create the str to give to the limit parameter in the
gffutils """
seqid_str = None
seq = egglib.Align()
with egglib.io.fasta_iter(ref_genome) as f:
for item in f:
if item.name == chrom:
seq.add_sample(item.name, item.sequence.str())
length = item.ls
seqid_str = chrom + ':1-%s' % length
break
return seqid_str, seq
def calc_pi(alignment, sequence):
piDict = {}
piList = []
a = egglib.Align(alignment)
for i in range(a.ns()):
a.sequence(i, sequence=a.sequence(i).upper())
seqIndex = a.find(sequence, strict=False)
for i in range(a.ns()):
if i != seqIndex:
tempAlign = egglib.Align.create([a[seqIndex], a[i]])
polyDict = tempAlign.polymorphism()
piDict[a.name(i)] = polyDict['Pi']
piList.append(float(polyDict['Pi']))
minPi = min(piList)
strainList = []
for strain in piDict:
if float(piDict[strain]) == minPi:
strainList.append(strain)
return strainList
def calc_stats(alignment, outgroup):
statDict = {}
a = egglib.Align(alignment)
for i in range(a.ns()):
a.sequence(i, sequence=a.sequence(i).upper())
if args.frame:
a.sequence(i, sequence=replace_stop(a.sequence(i)))
polyDict = a.polymorphism()
statDict['theta'] = polyDict['thetaW']
statDict['pi'] = polyDict['Pi']
statDict['tajimaD'] = polyDict['D']
if args.frame:
if len(a.sequence(1)) % 3 != 0:
print("The following alignment is not in frame:")
print(alignment)
return {}
polyDictBPP = a.polymorphismBPP(dataType=4)
statDict['piN'] = polyDictBPP['PiNS']
statDict['piS'] = polyDictBPP['PiS']
statDict['NS'] = polyDictBPP['NSsites']
statDict['S'] = polyDictBPP['Ssites']
if outgroup is not None:
for o in outgroup:
temp_a = a.extract(0, len(a.sequence(1)) - 3) # remove stop codon
otherOutgroups = outgroup[:]
otherOutgroups.remove(o)
for otherOutgroup in otherOutgroups:
index = temp_a.find(otherOutgroup, strict=False)
if index is not None:
del temp_a[index]
try:
temp_a.group(temp_a.find(o, strict=False), group=999)
except IndexError:
print("The following outgroup is not present in alignment")
print(o, a.find(o, strict=False))
sys.exit()
polyDictBPP = temp_a.polymorphismBPP(dataType=4)
statDict['MK_' + o] = polyDictBPP['MK']
statDict['NI_' + o] = polyDictBPP['NI']
return statDict
# GP2=metal
print "SCAFFOLD\tPAIR\tSTART\tEND\tDxy\tDa\tPi1\tPi2"
scafs = [
"1", "2", "3", "4", "5", "6", "7", "8", "9", "10", "11", "12", "13", "14",
"15", "16", "17", "18", "19", "20", "21", "22", "23", "24", "25", "26",
"27", "28", "29", "30", "31", "32", "33", "34", "35", "36", "37", "38",
"39", "40", "41", "42", "43", "44", "45", "46", "47"
]
#listed by doing ls HiQ_Sb_woNsSin_woSb32Sb7_indivscafs_fasta_concat/ | while read f; do g=`echo $f|sed 's/HiQ_Sb_woNsSin_woSb32Sb7_scaffold_//'| sed 's/.fasta//'`; echo -n $g"\",\""; done
#for fasta in os.listdir(path+"/"+scaffolds):
for scaf in scafs:
fasta = fastas + scaf + ".fasta"
data = egglib.Align(path + fasta, groups=False)
popdata = {}
for pop in pop_ids:
popdata[pop] = []
for seq in data:
seq.name = seq.name.replace(scaf + '_', '')
for pop in pop_ids:
if seq.name in pops[pop]:
popdata[pop].append(seq)
for pair in itertools.combinations(pop_ids, 2):
if sorted(pair) not in [
sorted(['WAca', 'CAWAca']),
sorted(['WAnm', 'NMWAnm']),
sorted(['AKEU', 'AK'])
]: #*****CHECK THIS!!!!
align1 = egglib.Align.create(popdata[pair[0]])
GP2 = [
"502931_1151576", "502931_1151572", "502931_1143066", "502931_1143067",
"502931_1143068", "502931_1151574", "502931_1143052", "502931_1143050",
"502931_1143051", "502931_1143078", "502931_1151580", "502931_1151581",
"502931_1151582", "502931_1143070", "502931_1143073", "502931_1151559",
"502931_1151560", "502931_1151561", "502931_1151562", "502931_1151564"
]
pops = {'GP1': GP1, 'GP2': GP2}
pop_ids = ['GP1', 'GP2']
print "scaffold\twindow\tgroup\tS\tthetaW\tPi\tD\tHe\tK"
dataset = dir.replace("concatscafs_fasta", "") #
for file in os.listdir(path + dir):
# print file
data = egglib.Align(path + dir + "/" + file, groups=False)
popdata = {}
for pop in pop_ids:
popdata[pop] = []
scaf = file.replace(dataset + "_scaffold_", "")
scaf = scaf.replace(".fasta", "")
print scaf
for seq in data:
seq.name = (seq.name).replace("scaffold_" + scaf + "_", "")
# print seq.name
for pop in pop_ids:
if seq.name in pops[pop]:
popdata[pop].append(seq)
# for name,seq in popdata['GP1']:
# print name,seq
def step(ext, dirname, names):
ext = ext.lower()
# this will make sure the extension is lowercase
for name in names:
if name.lower().endswith(ext):
my_align = egglib.io.from_fasta(name)
## index corresponds to the position of each sample in the list -1 (Python!!)
brau_list = [0, 47]
chlo_list = [1, 2]
clar_list = [3, 4]
defi_list = [5, 6, 39]
der_list = [7]
irano_list = [69, 70]
mix1_list = [9, 12, 13, 14]
mix2_list = [10, 11]
par_list = [17, 41, 66]
por1_list = [19, 21]
por2_list = [18, 22, 23]
praB_list = [25, 28]
praC_list = [24, 26, 27]
rad1_list = [15, 16, 20, 29, 30, 31, 32, 33, 35, 36, 37]
rad2_list = [34, 38, 40]
rud1_list = [45, 46, 56]
rud2_list = [42, 43, 44, 68]
sax_list = [8, 48]
stei_list = [49, 50, 51]
val_list = [52, 55, 57, 58, 59, 60, 61, 62, 63, 64, 65, 67, 53, 54]
# to make a new file I will create a new alignment
#rand_align = egglib.Align.create(align)
rand_align = egglib.Align()
# group_of_items = {1, 2, 3, 4} # a sequence or set will work here.
num_of_inds_per_sps = 2 # set the number to select here.
#list_of_random_items = random.sample(group_of_items, num_of_inds_per_sps)
# first_random_item = list_of_random_items[0]
# second_random_item = list_of_random_items[1]
rand_brau = random.sample(brau_list, num_of_inds_per_sps)
rand_chlo = random.sample(chlo_list, num_of_inds_per_sps)
rand_clar = random.sample(clar_list, num_of_inds_per_sps)
rand_defi = random.sample(defi_list, num_of_inds_per_sps)
rand_der = der_list
rand_irano = random.sample(irano_list, num_of_inds_per_sps)
rand_mix1 = random.sample(mix1_list, num_of_inds_per_sps)
rand_mix2 = random.sample(mix2_list, num_of_inds_per_sps)
rand_par = random.sample(par_list, num_of_inds_per_sps)
rand_par12 = par12_list
rand_por1 = random.sample(por1_list, num_of_inds_per_sps)
rand_por2 = random.sample(por2_list, num_of_inds_per_sps)
rand_praB = random.sample(praB_list, num_of_inds_per_sps)
rand_praC = random.sample(praC_list, num_of_inds_per_sps)
rand_rad1 = random.sample(rad1_list, num_of_inds_per_sps)
rand_rad2 = random.sample(rad2_list, num_of_inds_per_sps)
rand_rud1 = random.sample(rud1_list, num_of_inds_per_sps)
rand_rud2 = random.sample(rud2_list, num_of_inds_per_sps)
rand_sax = random.sample(sax_list, num_of_inds_per_sps)
rand_stei = random.sample(stei_list, num_of_inds_per_sps)
rand_val = random.sample(val_list, num_of_inds_per_sps)
brau = my_align.subset(rand_brau)
chlo = my_align.subset(rand_chlo)
clar = my_align.subset(rand_clar)
defi = my_align.subset(rand_defi)
der = my_align.subset(rand_der)
irano = my_align.subset(rand_irano)
mix1 = my_align.subset(rand_mix1)
mix2 = my_align.subset(rand_mix2)
par = my_align.subset(rand_par)
par12 = my_align.subset(rand_par12)
por1 = my_align.subset(rand_por1)
por2 = my_align.subset(rand_por2)
praB = my_align.subset(rand_praB)
praC = my_align.subset(rand_praC)
rad1 = my_align.subset(rand_rad1)
rad2 = my_align.subset(rand_rad2)
rud1 = my_align.subset(rand_rud1)
rud2 = my_align.subset(rand_rud2)
sax = my_align.subset(rand_sax)
stei = my_align.subset(rand_stei)
val = my_align.subset(rand_val)
# add on the alignment the random sequences
rand_align.add_samples(brau)
rand_align.add_samples(chlo)
rand_align.add_samples(clar)
rand_align.add_samples(defi)
rand_align.add_samples(der)
rand_align.add_samples(mix1)
rand_align.add_samples(mix2)
rand_align.add_samples(par)
rand_align.add_samples(par12)
rand_align.add_samples(por1)
rand_align.add_samples(por2)
rand_align.add_samples(praB)
rand_align.add_samples(praC)
rand_align.add_samples(rad1)
rand_align.add_samples(rad2)
rand_align.add_samples(rud1)
rand_align.add_samples(rud2)
rand_align.add_samples(sax)
rand_align.add_samples(stei)
rand_align.add_samples(val)
rand_align.add_samples(irano)
# now the brau subset has all sequences in the order I want
rand_align.to_fasta(fname=name + '.fa')
nex = rand_align.nexus()
nexfile = open(name + '.nexus', 'w')
nexfile.write(nex)
statDict['theta'] = polyDict['thetaW']
statDict['pi'] = polyDict['Pi']
statDict['tajimaD'] = polyDict['D']
statDict['FayWuH'] = polyDict['H']
return statDict
alignment, winWidth, winStep, outgroup = get_arguments(sys.argv[1:])
if alignment is None:
usage()
sys.exit()
outfile = open('windowStats_' + os.path.splitext(alignment)[0] + '.txt', 'w')
outfile.write("Start\tStop\tTheta\tPi\tTajimasD\tFay&WuH\n")
align = egglib.Align(alignment)
for i in range(align.ns()):
align.sequence(i, sequence=align.sequence(i).upper())
if outgroup is not None:
align.group(align.find(outgroup, strict=False), group=999)
start = 0
stop = winWidth
location = []
TD = []
for window in align.slider(winWidth, winStep):
stats = calc_stats(window)
start += winStep
stop += winStep
outfile.write("%i\t%i\t%s\t%s\t%s\t%s\n" %
def extract_cds_align(vcf, min_dp, max_dp, sample_list, gff, gene_id, cds_dict, filtered=True):
"""
Iterator that goes site by site through vcf gene region and returns
a CDS align and positions of sites in that align
Returns:
object: list
"""
gene_cds_aligns = []
#for transcript in cds_dict[gene_id]:
transcript = cds_dict[gene_id].keys()[0]
cds_pos_list = []
for coords in cds_dict[gene_id][transcript]:
if type(coords) is tuple:
cds_pos_list += range(coords[0], coords[1] + 1)
len_bp = len(cds_pos_list)
cds_align = egglib.Align(nsit=len_bp)
for sample in sample_list:
cds_align.add_sample(sample + '_1', data='N'*len_bp)
cds_align.add_sample(sample + '_2', data='N'*len_bp)
align_pos = -1
indel_end = 0
gene_region = extract_gene(vcf, gff, gene_id)
for site in gene_region:
if site.POS not in cds_pos_list: # only extract genic sites in CDS blocks
continue
align_pos += 1
if site.REF == 'N':
# ref_N += 1
continue
if site.is_indel and site.aaf != 0.0:
if len(site.REF) > len(site.ALT):
indel_end = site.POS + len(site.REF) - 1
continue
# indels += 1
else:
continue
if len(site.ALT) >= 1 and site.ALT[-1] == '*': # SNPs at spanning deletion
# spanning_deletion += 1
continue
all_dp = [x['DP'] for x in site.samples] # get the genotype depths
if None in all_dp: # Exclude sites where a genotype DP field is set to '.'
# low_call_rate += 1
continue
mean_dp = np.mean(all_dp)
if mean_dp < min_dp or mean_dp > max_dp: # depth filter
# extreme_depth += 1
continue
if 0 in all_dp or site.call_rate < 1.0: # only consider sites where all samples have coverage
# low_call_rate += 1
continue
if site.FILTER is not None:
if site.FILTER == "REPEAT" or "REPEAT" in site.FILTER: # exclude sites in repeat regions
# repeat_sites += 1
continue
if site.is_monomorphic:
if site.POS > indel_end:
call_to_bases(site, align_pos, cds_align, sample_list)
indel_end = 0
continue
else:
continue
if site.is_indel and site.aaf == 0.0:
if site.POS > indel_end:
call_to_bases(site, align_pos, cds_align, sample_list)
indel_end = 0
# valid_sites += 1
continue
else:
continue
if site.is_snp:
if len(site.ALT) > 1:
# multiallelic_snp += 1
continue
if site.aaf == 1.0 or site.aaf == 0.0: # only want SNPs polymorphic in our sample
if site.POS > indel_end:
call_to_bases(site, align_pos, cds_align, sample_list)
continue
else:
continue
if filtered:
if site.FILTER == 'PASS' and site.POS > indel_end:
call_to_bases(site, align_pos, cds_align, sample_list)
continue
else:
# failed_snp += 1
continue
else:
if site.POS > indel_end:
call_to_bases(site, align_pos, cds_align, sample_list)
else:
continue
else:
problem_site = site.CHROM + '\t' + str(site.POS)
error_message = 'Could not assign ' + problem_site + ' to site type'
sys.exit(error_message)
if cds_dict[gene_id][transcript][-2] == '-': # reverse-complement when on '-' strand
cds_align = rc_align(cds_align)
cds_pos_list.reverse()
cds_align_nostop = cds_align.extract(0, cds_align.ls - 3) # drop the stop codon at the end
gene_cds_aligns.append((cds_align_nostop, cds_pos_list[0:-3]))
return cds_align_nostop, cds_pos_list[0:-3]
