def open_instrument(self):
"""Doc."""
try:
self._inst = uc480.UC480_Camera(serial=self.serial.encode(),
reopen_policy="new")
except Exception as exc:
# general 'Exception' is due to bad exception handeling in instrumental-lib...
raise IOError(f"{self.log_ref} disconnected - {exc}")
def search_live_image(source=source, source_mag=10., flake_mag=126.5):
try:
sys.path.append(r'D:\Nonlinear_setup\Python_codes')
global cam, bg
bg = np.load(
'D:/Nonlinear_setup/Python_codes/search_live_image_bg.npy')
bg = 1. * bg / np.max(bg)
try:
from instrumental.drivers.cameras import uc480
cam = uc480.UC480_Camera()
except:
pass
clahe = cv2.createCLAHE(clipLimit=.1, tileGridSize=(80, 80))
def capture_image():
img = cam.latest_frame()
img = (img / bg).astype(np.uint8)
# img = clahe.apply(img)
img = cv2.equalizeHist(img)
img = filters.minimum_filter(img, 3)
# img = cv2.fastNlMeansDenoising(img)
return np.fliplr(img)
def press(event):
if event.key == 'b':
get_live_image_bg()
global bg
bg = np.load(
'D:/Nonlinear_setup/Python_codes/search_live_image_bg.npy')
bg = 1. * bg / np.max(bg)
cam.auto_blacklevel = 1
cam.auto_exposure = 1
cam.auto_framerate = 1
cam.auto_gain = 1
cam.start_live_video()
# cam.start_capture()
flake = capture_image()
search_single_image(flake, source, source_mag, flake_mag)
_fig.canvas.mpl_connect('key_press_event', press)
plt.pause(1e-6)
while True:
flake = capture_image()
search_single_image(flake,
source,
source_mag,
flake_mag,
update=True)
plt.pause(1e-6)
except KeyboardInterrupt:
cam.stop_live_video()
# -*- coding: utf-8 -*-
"""
Created on Tue Nov 13 11:14:13 2018
@author: USUARIO
"""
import sys
import numpy as np
import matplotlib.pyplot as plt
from instrumental.drivers.cameras import uc480
import time
sys.path.append('C:\Program Files\Thorlabs\Scientific Imaging\ThorCam')
myCamera = uc480.UC480_Camera()
print('Model {}'.format(myCamera.model))
print('Serial number {}'.format(myCamera.serial))
#raw_image = myCamera.grab_image(exposure_time='50 ms')
myCamera.start_live_video('10 Hz')
time.sleep(1)
raw_image = myCamera.latest_frame()
r = raw_image[:, :, 0]
g = raw_image[:, :, 1]
b = raw_image[:, :, 2]
self.focusTimer.stop()
self.camera.close()
if self.standAlone is True:
zMoveTo(self.actuator, 0)
if __name__ == '__main__':
print('Focus lock module running in stand-alone mode')
app = QtGui.QApplication([])
cam = uc480.UC480_Camera()
DEVICENUMBER = 0x1
adw = ADwin.ADwin(DEVICENUMBER, 1)
scan.setupDevice(adw)
# initialize fpar_52 (z) ADwin position parameters
pos_zero = tools.convert(0, 'XtoU')
adw.Set_FPar(52, pos_zero)
zMoveTo(adw, 10)
win = focusWidget(cam, adw)
win.fworker.standAlone = True
from pygame import mixer
mixer.init()
sys.path.append(os.path.abspath(r"D:\WMP_setup\Python_codes"))
sys.path.append(os.path.abspath(r"D:\Nonlinear_setup\Python_codes"))
from neo_common_code import *
from instrumental.drivers.cameras import uc480
from anal_measure_beam_profile_with_camera import *
global cam
try:
if not cam.model == 'C1285R12M':
raise Exception
else:
print('Camera C1285R12M already online.')
except:
cam = uc480.UC480_Camera('C1285R12M')
print('Camera C1285R12M online.')
cam.auto_exposure = False
cam.auto_framerate = False
cam.auto_gain = False
try:
import TL_slider_2 #sc
def block_sc():
TL_slider_2.block_laser_2()
def unblock_sc():
TL_slider_2.unblock_laser_2()
except:
print "SC shutter not connected"
def __init__(self, *args, **kwargs):
super().__init__(*args, **kwargs)
self.setWindowTitle('PyFLUX')
self.cwidget = QtGui.QWidget()
self.setCentralWidget(self.cwidget)
# Actions in menubar
menubar = self.menuBar()
fileMenu = menubar.addMenu('Measurement')
self.psfMeasAction = QtGui.QAction('PSF measurement', self)
self.psfMeasAction.setStatusTip('Routine to measure one MINFLUX PSF')
fileMenu.addAction(self.psfMeasAction)
self.psfMeasAction.triggered.connect(self.psfMeasurement)
self.minfluxMeasAction = QtGui.QAction('MINFLUX measurement', self)
self.minfluxMeasAction.setStatusTip(
'Routine to perform a tcspc-MINFLUX measurement')
fileMenu.addAction(self.minfluxMeasAction)
# GUI layout
grid = QtGui.QGridLayout()
self.cwidget.setLayout(grid)
# Dock Area
dockArea = DockArea()
grid.addWidget(dockArea, 0, 0)
# Scan
scanDock = Dock('Confocal scan', size=(1, 1))
DEVICENUMBER = 0x1
self.adw = drivers.ADwin.ADwin(DEVICENUMBER, 1)
scan.setupDevice(self.adw)
self.scanWidget = scan.scanWidget(self.adw)
scanDock.addWidget(self.scanWidget)
dockArea.addDock(scanDock, 'left')
# tcspc measurement
tcspcDock = Dock("Time-correlated single-photon counting")
ph = drivers.picoharp.PicoHarp300()
self.tcspcWidget = tcspc.tcspcWidget(ph)
tcspcDock.addWidget(self.tcspcWidget)
dockArea.addDock(tcspcDock, 'bottom', scanDock)
# focus lock
focusDock = Dock("Focus Lock")
uc480Camera = uc480.UC480_Camera()
self.focusWidget = focus.focusWidget(uc480Camera, self.adw)
focusDock.addWidget(self.focusWidget)
dockArea.addDock(focusDock, 'right')
# xy drift
xyDock = Dock("xy drift control")
andorCamera = ccd.CCD()
self.xyWidget = xy.xyWidget(andorCamera)
xyDock.addWidget(self.xyWidget)
xyDock.addWidget(self.xyWidget)
dockArea.addDock(xyDock, 'top', focusDock)
# threads
self.scanThread = QtCore.QThread(self)
self.scanThread.start()
self.scanWidget.scworker.moveToThread(self.scanThread)
self.focusThread = QtCore.QThread(self)
self.focusThread.start()
self.focusWidget.fworker.moveToThread(self.focusThread)
self.xyThread = QtCore.QThread(self)
self.xyThread.start()
self.xyWidget.xyworker.moveToThread(self.xyThread)
# self.tcspcThread = QtCore.QThread(self)
# self.tcspcThread.start()
# self.tcspcWidget.tcspcworker.moveToThread(self.tcspcThread)
# sizes to fit my screen properly
self.scanWidget.setMinimumSize(1000, 550)
self.xyWidget.setMinimumSize(800, 300)
self.move(1, 1)
