# FLT3
for rtkf_name in ['FL']:
add_monomer_synth_deg(rtkf_name)
rtk_cascade = [
('FLT3', {'Y843': ['FL']}),
]
generate_pathway(model, rtk_cascade)
# ERK
for ras_name in ['HRAS', 'KRAS', 'NRAS']:
add_monomer_synth_deg(ras_name, nsites=['N'])
add_activation(
model, ras_name, 'N', 'nucleotide_exchange',
['FLT3__Y843_p']
)
mapk_cascade = [
('RAF1', {'S338': ['KRAS__N_gtp', 'HRAS__N_gtp', 'NRAS__N_gtp']}),
('BRAF', {'S447': ['KRAS__N_gtp', 'HRAS__N_gtp', 'NRAS__N_gtp']}),
('MAP2K1', {'S218_S222': ['RAF1__S338_p', 'BRAF__S447_p']}),
('MAP2K2', {'S222_S226': ['RAF1__S338_p', 'BRAF__S447_p']}),
('MAPK1', {'T185_Y187': ['MAP2K1__S218_p__S222_p',
'MAP2K2__S222_p__S226_p']}),
('MAPK3', {'T202_Y204': ['MAP2K1__S218_p__S222_p',
'MAP2K2__S222_p__S226_p']}),
]
generate_pathway(model, mapk_cascade)
add_abundance_observables(model)
('EGFR', {
'Y1173': ['EGF']
}),
('ERBB2', {
'Y1248': ['EGF']
}),
]
generate_pathway(model, erbb_cascade)
active_rtks = ['EGFR__Y1173_p', 'ERBB2__Y1248_p']
stat_rtks = ['EGFR__Y1173_p', 'ERBB2__Y1248_p']
# MAPK
for ras_name in ['HRAS', 'KRAS', 'NRAS']:
add_monomer_synth_deg(ras_name, nsites=['N'])
add_activation(model, ras_name, 'N', 'nucleotide_exchange', active_rtks)
mapk_cascade = [
('RAF1', {
'S338': ['KRAS__N_gtp', 'HRAS__N_gtp', 'NRAS__N_gtp']
}),
('BRAF', {
'S445': ['KRAS__N_gtp', 'HRAS__N_gtp', 'NRAS__N_gtp']
}),
('ARAF', {
'S299': ['KRAS__N_gtp', 'HRAS__N_gtp', 'NRAS__N_gtp']
}),
('MAP2K1', {
'S218_S222': ['RAF1__S338_p', 'BRAF__S445_p', 'ARAF__S299_p']
}),
('MAP2K2', {