def protocol(self):
self.robot.get_serial_ports_list()
self.robot.home()
tiprack = containers_load(
self.robot,
'tiprack-200ul', # container type
'A1', # slot
'tiprack' # user-defined name
)
plate = containers_load(
self.robot,
'96-flat',
'B1',
'plate'
)
trash = containers_load(
self.robot,
'point',
'C2',
'trash'
)
trough = containers_load(
self.robot,
'trough-12row',
'B2',
'trough'
)
p200 = pipette.Pipette(
self.robot,
name="p200",
trash_container=trash,
tip_racks=[tiprack],
max_volume=200,
min_volume=0.5,
axis="b",
channels=1
)
self.robot.clear_commands()
# distribute
p200.pick_up_tip(tiprack[0])
p200.aspirate(96 * 2, trough[0])
for i in range(96):
p200.dispense(2, plate[i]).touch_tip()
p200.drop_tip(tiprack[0])
p200.pick_up_tip(tiprack[1])
for i in range(96):
p200.aspirate(2, plate[95 - i])
p200.dispense(trough[0])
p200.drop_tip(tiprack[1])
def test_deck_setup(robot):
deck = robot.deck
pip = pipette.Pipette(robot, mount='left', max_volume=300, ul_per_mm=18.0)
# Check that the fixed trash has loaded on to the pipette
trash = pip.trash_container
tiprack = containers_load(robot, 'tiprack-10ul', '5')
assert isinstance(tiprack, Container)
assert isinstance(deck, Deck)
# Check that well location is the same on the robot as the pipette
assert robot._deck['12']['tall-fixed-trash'][0] == trash
assert deck.has_container(tiprack)
def test_protocol_head(robot):
trash = containers_load(robot, 'point', '1', 'myTrash')
tiprack = containers_load(robot, 'tiprack-10ul', '5')
p200 = pipette.Pipette(
robot,
name='myPipette',
trash_container=trash,
tip_racks=[tiprack],
max_volume=200,
min_volume=10, # These are variable
ul_per_mm=18.0,
mount='left',
channels=1)
instruments_list = robot.get_instruments()
assert instruments_list[0] == ('left', p200)
instruments_list = robot.get_instruments('myPipette')
assert instruments_list[0] == ('left', p200)
def test_serial_dilution(robot):
plate = load(robot, '96-flat', '2', 'plate')
tiprack = load(
robot,
'tiprack-200ul', # container type from library
'1', # slot on deck
'tiprack' # calibration reference for 1.2 compatibility
)
trough = load(robot, 'trough-12row', '5', 'trough')
trash = load(robot, 'point', '3', 'trash')
p200 = pipette.Pipette(
robot,
ul_per_mm=18.5,
trash_container=trash,
tip_racks=[tiprack],
min_volume=10,
max_volume=200, # These are variable
mount='left',
channels=1)
p200.calibrate_plunger(top=0, bottom=10, blow_out=12, drop_tip=13)
for t, col in enumerate(plate.cols):
p200.pick_up_tip(tiprack[t])
p200.aspirate(10, trough[t])
p200.dispense(10, col[0])
for well, next_well in zip(col[:-1], col[1:]):
p200.aspirate(10, well)
p200.dispense(10, next_well)
p200.mix(repetitions=3, volume=10, location=next_well)
p200.drop_tip(trash)