def _format_variants(self, variant, index, case_obj, add_all_info=False):
"""Return a Variant object
Format variant make a variant that includes enough information for
the variant view.
If add_all_info then all transcripts will be parsed
Args:
variant (cython2.Variant): A variant object
index (int): The index of the variant
case_obj (puzzle.models.Case): A case object
"""
header_line = self.head.header
# Get the individual ids for individuals in vcf file
vcf_individuals = set([ind_id for ind_id in self.head.individuals])
#Create a info dict:
info_dict = dict(variant.INFO)
chrom = variant.CHROM
if chrom.startswith('chr') or chrom.startswith('CHR'):
chrom = chrom[3:]
variant_obj = Variant(
CHROM=chrom,
POS=variant.POS,
ID=variant.ID,
REF=variant.REF,
ALT=variant.ALT[0],
QUAL=variant.QUAL,
FILTER=variant.FILTER,
)
variant_obj._set_variant_id()
logger.debug("Creating a variant object of variant {0}".format(
variant_obj.variant_id))
variant_obj.index = index
logger.debug("Updating index to: {0}".format(index))
########### Get the coordinates for the variant ##############
variant_obj.start = variant.start
variant_obj.stop = variant.end
#SV variants needs to be handeled a bit different since the can be huge
#it would take to much power to parse all vep/snpeff entrys for these.
if self.variant_type == 'sv':
variant_obj.stop = int(info_dict.get('END', variant_obj.POS))
self._add_sv_coordinates(variant_obj)
variant_obj.sv_type = info_dict.get('SVTYPE')
# Special for FindSV software:
# SV specific tag for number of occurances
occurances = info_dict.get('OCC')
if occurances:
logger.debug("Updating occurances to: {0}".format(occurances))
variant_obj['occurances'] = float(occurances)
variant_obj.add_frequency('OCC', occurances)
else:
self._add_thousand_g(variant_obj, info_dict)
self._add_cadd_score(variant_obj, info_dict)
self._add_genetic_models(variant_obj, info_dict)
self._add_transcripts(variant_obj, info_dict)
self._add_exac(variant_obj, info_dict)
self._add_hgnc_symbols(variant_obj)
if add_all_info:
self._add_genotype_calls(variant_obj, str(variant), case_obj)
self._add_compounds(variant_obj, info_dict)
self._add_gmaf(variant_obj, info_dict)
self._add_genes(variant_obj)
##### Add consequences ####
self._add_consequences(variant_obj, str(variant))
self._add_most_severe_consequence(variant_obj)
self._add_impact_severity(variant_obj)
self._add_rank_score(variant_obj, info_dict)
variant_obj.set_max_freq()
return variant_obj
