def merge_mvf(args):
"""Main method"""
args.qprint("Running MergeMVF")
if any(fpath.endswith('.gz') for fpath in args.mvf):
print("WARNING! Running MergeMVF with gzipped input files is "
"extremely slow and strongly discouraged.")
concatmvf = MultiVariantFile(args.out, 'write', overwrite=args.overwrite)
# Copy the first file's metadata
args.qprint("Reading First File and Establishing Output")
if args.main_header_file:
if args.main_header_file not in args.mvf:
raise RuntimeError("{} not found in files".format(
args.main_header_file))
else:
args.main_header_file = args.mvf.index(args.main_header_file)
else:
args.main_header_file = 0
first_mvf = MultiVariantFile(args.mvf[args.main_header_file], 'read')
concatmvf.metadata = first_mvf.metadata.copy()
# Open each MVF file, read headers to make unified header
transformers = []
mvfmetadata = []
concatmvf_reverse_contig = dict(
(x['label'], k) for (k, x) in concatmvf.metadata['contigs'].items())
inputfiles = []
for mvfname in args.mvf:
args.qprint("Reading headers from {}".format(mvfname))
# This will create a dictionary of samples{old:new}, contigs{old:new}
args.qprint("Processing Headers and Indexing: {}".format(mvfname))
transformer = MvfTransformer()
mvf = MultiVariantFile(mvfname,
'read',
contigindex=(not args.skip_index))
if args.skip_index:
mvf.read_index_file()
mvf.reset_max_contig_id()
mvfmetadata.append(mvf.metadata)
for i, label in enumerate(mvf.get_sample_labels()):
if label not in concatmvf.get_sample_labels():
concatmvf.metadata['labels'].append(label)
concatmvf.metadata['samples'][
concatmvf.metadata['labels'].index(label)] = {
'label': label
}
# if concatmvf.metadata['labels'].index(label) != i:
transformer.set_label(i, concatmvf.metadata['labels'].index(label))
for contigid, contigdata in iter(mvf.metadata['contigs'].items()):
if contigdata['label'] not in concatmvf_reverse_contig:
newid = (contigid
if contigid not in concatmvf.metadata['contigs'] else
concatmvf.get_next_contig_id())
concatmvf.metadata['contigs'][newid] = contigdata
concatmvf_reverse_contig[contigdata['label']] = newid
else:
newid = concatmvf_reverse_contig[contigdata['label']]
transformer.set_contig(contigid, newid)
transformers.append(transformer)
inputfiles.append(mvf)
# Write output header
args.qprint("Writing headers to merge output")
concatmvf.reset_ncol()
concatmvf.write_data(concatmvf.get_header())
contigs = concatmvf.metadata['contigs']
# Now loop through each file
blank_entry = '-' * len(concatmvf.metadata['samples'])
for current_contig in contigs:
contig_merged_entries = {}
args.qprint("Merging Contig: {}".format(current_contig))
for ifile, mvffile in enumerate(inputfiles):
if current_contig not in transformers[ifile].contigs:
continue
localcontig = transformers[ifile].contigs[current_contig]
for chrom, pos, allelesets in mvffile.itercontigentries(
localcontig, decode=True):
if pos not in contig_merged_entries:
contig_merged_entries[pos] = blank_entry[:]
for j, base in enumerate(allelesets[0]):
xcoord = transformers[ifile].labels_rev[j]
if contig_merged_entries[pos][xcoord] != '-':
if contig_merged_entries[pos][xcoord] == base:
continue
if base == '-' or base == 'X':
continue
raise RuntimeError(
"Merging columns have two different bases: {} {} {}"
.format(pos, contig_merged_entries[pos][xcoord],
base))
contig_merged_entries[pos] = (
contig_merged_entries[pos][:xcoord] + base +
contig_merged_entries[pos][xcoord + 1:])
concatmvf.write_entries(
((current_contig, coord, (entry, ))
for coord, entry in sorted(contig_merged_entries.items())),
encoded=False)
args.qprint("Entries written for contig {}: {}".format(
current_contig, len(contig_merged_entries)))
return ''
def mvf_join(args):
"""Main method"""
concatmvf = MultiVariantFile(args.out, 'write', overwrite=args.overwrite)
# Copy the first file's metadata
if args.main_header_file:
if args.main_header_file not in args.mvf:
raise RuntimeError("{} not found in files".format(
args.main_header_file))
else:
args.main_header_file = args.mvf.index(args.main_header_file)
else:
args.main_header_file = 0
first_mvf = MultiVariantFile(args.mvf[args.main_header_file], 'read')
concatmvf.metadata = first_mvf.metadata.copy()
# Open each MVF file, read headers to make unified header
transformers = []
for mvfname in args.mvf:
# This will create a dictionary of samples{old:new}, contigs{old:new}
transformer = MvfTransformer()
mvf = MultiVariantFile(mvfname, 'read')
mvf.reset_max_contig_id()
for i, label in enumerate(mvf.get_sample_labels()):
if label not in concatmvf.get_sample_labels():
concatmvf.metadata['labels'].append(label)
concatmvf.metadata['samples'][
concatmvf.metadata['labels'].index(label)] = {
'label': label
}
if concatmvf.metadata['labels'].index(label) != i:
transformer.set_label(
i, concatmvf.metadata['labels'].index(label))
for contigid, contigdata in iter(mvf.metadata['contigs'].items()):
if contigdata['label'] not in [
concatmvf.metadata['contigs'][x]['label']
for x in concatmvf.metadata['contigs']
]:
newid = (contigid not in concatmvf.metadata['contigs']
and contigid or concatmvf.get_next_contig_id())
concatmvf.metadata['contigs'][newid] = contigdata
else:
for concatid, concatdata in (
concatmvf.metadata['contigs'].items()):
if contigdata['label'] == concatdata['label']:
newid = concatid
break
if newid != contigid:
transformer.set_contig(contigid, newid)
transformers.append(transformer)
# Write output header
concatmvf.write_data(concatmvf.get_header())
# Now loop through each file
entries = []
nentries = 0
for ifile, mvfname in enumerate(args.mvf):
if not args.quiet:
sys.stderr.write("Processing {} ...\n".format(mvfname))
transformer = transformers[ifile]
mvf = MultiVariantFile(mvfname, 'read')
for contigid, pos, allelesets in mvf.iterentries(decode=False,
quiet=args.quiet):
if transformer.labels:
allelesets = [mvf.decode(x) for x in allelesets]
for j, alleles in enumerate(allelesets):
allelesets[j] = concatmvf.encode(''.join([
x in transformer.labels
and alleles[transformer.labels[x]] or alleles[x]
for x in range(len(alleles))
]))
if transformer.contigs:
contigid = (contigid in transformer['contigs']
and transformer['contigs'][contigid] or contigid)
entries.append((contigid, pos, allelesets))
nentries += 1
if nentries == args.line_buffer:
concatmvf.write_entries(entries)
entries = []
nentries = 0
if entries:
concatmvf.write_entries(entries)
entries = []
nentries = 0
if not args.quiet:
sys.stderr.write("done\n")
return ''
def vcf2mvf(args=None):
"""Main method for vcf2mvf"""
sepchars = dict([("TAB", "\t"), ("SPACE", " "), ("DBLSPACE", " "),
("COMMA", ","), ("MIXED", None)])
args.fieldsep = sepchars[args.field_sep]
# ESTABLISH VCF
args.qprint("Opening input VCF: {}".format(args.vcf))
vcf = VariantCallFile(args.vcf, indexcontigs=(not args.no_autoindex))
# ESTABLISH MVF
args.qprint("Establishing output MVF: {}".format(args.out))
mvf = MultiVariantFile(args.out, 'write', overwrite=args.overwrite)
# PROCESS CONTIG INFO
args.qprint("Processing VCF headers.")
vcfcontigs = vcf.metadata['contigs'].copy()
args.qprint("{} contigs found.".format(len(vcfcontigs)))
contig_translate = {}
if args.contig_ids:
for cid, cvcf, cmvf in (x.split(';') for x in args.contig_ids):
try:
cid = int(cid)
except ValueError:
pass
assert cvcf in [vcfcontigs[x]['label'] for x in vcfcontigs]
for vid in vcfcontigs:
if vcfcontigs[vid]['label'] == cvcf:
contig_translate[cvcf] = [cid, cmvf]
if cid in mvf.metadata['contigs']:
raise RuntimeError(
'Contig id {} is not unique'.format(cid))
mvf.metadata['contigs'][cid] = vcfcontigs[vid].copy()
if cmvf in mvf.get_contig_labels():
raise RuntimeError(
'Contig label {} is not unique'.format(cmvf))
mvf.metadata['contigs'][cid]['label'] = cmvf[:]
mvf.reset_max_contig_id()
args.qprint("Processing contigs.")
static_contig_ids = mvf.get_contig_ids()
for vcid in vcfcontigs:
vlabel = vcfcontigs[vcid]['label']
if vlabel not in static_contig_ids:
if ((is_int(vlabel) or len(vlabel) < 3) and
vlabel not in static_contig_ids):
newid = vlabel[:]
else:
newid = mvf.get_next_contig_id()
mvf.metadata['contigs'][newid] = vcfcontigs[vcid].copy()
static_contig_ids.append(newid)
contig_translate[vlabel] = [newid, vlabel]
mvf.reset_max_contig_id()
new_contigs = [(x, mvf.metadata['contigs'][x]['label'])
for x in mvf.metadata['contigs']]
if args.skip_contig_label_check is False:
args.qprint("Checking contigs for label/id overlap errors.")
xids = [x[0] for x in new_contigs]
xlabels = [x[1] for x in new_contigs]
for i, (newid, newlabel) in enumerate(new_contigs):
if newid in xlabels[:i] or newid in xlabels[i+1:]:
raise RuntimeError("Error contig id {} is the same as"
" the label for another contig"
" ({})".format(
newid, xlabels))
if newlabel in xids[:i] or newlabel in xids[i+1:]:
raise RuntimeError("Error contig label {} is the same"
"as the id for another contig"
"({})".format(
newlabel, xlabels))
# PROCESS SAMPLE INFO
args.qprint("Processing samples.")
samplelabels = [args.ref_label] + vcf.metadata['samples'][:]
if args.alleles_from:
args.alleles_from = args.alleles_from.split(':')
samplelabels += args.alleles_from
if args.sample_replace:
newsample = [x.split(':') if ':' in tuple(x) else tuple([x, x])
for x in args.sample_replace]
unmatched = [x for x in enumerate(samplelabels)]
for old, new in newsample:
labelmatched = False
for j, (i, name) in enumerate(unmatched):
if old in name:
samplelabels[i] = new
labelmatched = j
break
if labelmatched is not False:
del unmatched[labelmatched]
mvf.metadata['labels'] = samplelabels[:]
for i, label in enumerate(samplelabels):
mvf.metadata['samples'][i] = {'label': label}
mvf.metadata['ncol'] = len(mvf.metadata['labels'])
mvf.metadata['sourceformat'] = vcf.metadata['sourceformat']
# WRITE MVF HEADER
mvf.write_data(mvf.get_header())
mvfentries = []
nentry = 0
args.qprint("Processing VCF entries.")
for vcfrecord in vcf.iterentries(args):
# try:
mvf_alleles = encode_mvfstring(''.join(vcfrecord['genotypes']))
if args.out_flavor in ('dnaqual',):
qual_alleles = encode_mvfstring(''.join(vcfrecord['qscores']))
if mvf_alleles:
mvfentries.append(
(contig_translate.get(vcfrecord['contig'])[0],
vcfrecord['coord'],
((mvf_alleles, qual_alleles) if
args.out_flavor in ('dnaqual',) else
(mvf_alleles,))))
nentry += 1
if nentry == args.line_buffer:
mvf.write_entries(mvfentries, encoded=True)
mvfentries = []
nentry = 0
# except Exception as exception:
if mvfentries:
mvf.write_entries(mvfentries)
mvfentries = []
return ''
