def add_tiles_and_assign_dna(design):
# left tiles
left_left = 11
left_right = 32
for col, seq in zip(range(2, 18, 2), tile_dna_seqs):
bot_helix = top_helix + 1
ss_top = sc.Domain(helix=top_helix,
forward=True,
start=left_left,
end=left_right)
ss_bot = sc.Domain(helix=bot_helix,
forward=False,
start=left_left,
end=left_right)
tile = sc.Strand(domains=[ss_bot, ss_top], color=sc.Color(0, 0, 0))
design.add_strand(tile)
design.assign_dna(tile, seq)
# right tiles
right_left = 480
right_right = 501
for top_helix, seq in zip(range(2, 18, 2), tile_dna_seqs):
bot_helix = top_helix + 1
ss_top = sc.Domain(helix=top_helix,
forward=True,
start=right_left,
end=right_right)
ss_bot = sc.Domain(helix=bot_helix,
forward=False,
start=right_left,
end=right_right)
tile = sc.Strand(domains=[ss_bot, ss_top], color=sc.Color(0, 0, 0))
design.add_strand(tile)
design.assign_dna(tile, seq)
def main():
blue = sc.Color(r=0, g=0, b=255)
black = sc.Color(r=0, g=0, b=0)
red = sc.Color(r=255, g=0, b=0)
green = sc.Color(r=0, g=150, b=0)
cols = 8
rows = 8
# helices = [sc.Helix(major_tick_distance=10) for _ in range(rows+1)]
strands = []
for col in range(cols):
for row in range(rows):
helix = row
forward = row % 2 == 0
if forward:
offset = col * 20
ss1 = sc.Domain(helix, forward, offset, offset + 20)
ss2 = sc.Domain(helix + 1, forward, offset + 20, offset + 40)
else:
offset = col * 20
ss1 = sc.Domain(helix, forward, offset + 30, offset + 50)
ss2 = sc.Domain(helix + 1, forward, offset + 10, offset + 30)
if forward:
color = blue if col % 2 == 0 else black
else:
color = red if col % 2 == 0 else green
strand = sc.Strand([ss1, ss2], color=color)
strands.append(strand)
# design = sc.DNADesign(helices=helices, strands=strands, grid=sc.square)
design = sc.DNADesign(major_tick_distance=10,
strands=strands,
grid=sc.square)
return design
def add_tiles_and_assign_dna(design):
# left tiles
left_left = 11
left_right = 32
for top_helix, seq in zip(range(2, 18, 2), tile_dna_seqs):
bot_helix = top_helix + 1
ss_top = sc.Domain(helix=top_helix,
forward=True,
start=left_left,
end=left_right)
ss_bot = sc.Domain(helix=bot_helix,
forward=False,
start=left_left,
end=left_right)
idt = sc.IDTFields(name=f'tile-left-{top_helix}-{bot_helix}',
scale='25nm',
purification='STD')
tile = sc.Strand(domains=[ss_bot, ss_top],
color=sc.Color(0, 0, 0),
idt=idt)
design.add_strand(tile)
design.assign_dna(tile, seq)
# right tiles
right_left = 480
right_right = 501
for top_helix, seq in zip(range(2, 18, 2), tile_dna_seqs):
bot_helix = top_helix + 1
ss_top = sc.Domain(helix=top_helix,
forward=True,
start=right_left,
end=right_right)
ss_bot = sc.Domain(helix=bot_helix,
forward=False,
start=right_left,
end=right_right)
idt = sc.IDTFields(name=f'tile-right-{top_helix}-{bot_helix}',
scale='25nm',
purification='STD')
tile = sc.Strand(domains=[ss_bot, ss_top],
color=sc.Color(0, 0, 0),
idt=idt)
design.add_strand(tile)
design.assign_dna(tile, seq)