def add_newlinks_gamma_to_gamma(path, args):
""" function to add new links to above all of the betas """
# Initialize default values
path.reinit()
# make a copy of the yvar and xvar for iteration, because I am going to be
# messing with both lists
_xvar = list(path.xvar)
# Iterate over all exogenous variables (cols of gamma), turn them into an
# endogenous variable 1 at a time and then add all betas
for col, target in enumerate(_xvar):
# Determine if gene has multiple isoforms
tCnt = utils.isoCount(target)
# Convert exogenous varaible to an endogenous variable
path.convert_ExogToEndog(target)
# Now iterate over the new endogenous variable list (cols of gamma) and
# add links 1 at a time to gamma
for index, gene in enumerate(path.xvar):
model_type = "Adding Newlinks Gamma to Gamma :\n {0} -> {1}".format(gene, target)
# Determine if gene has multiple isoforms
isoCnt = utils.isoCount(gene)
# Skip when one gene is already regulating the other
path.gamma[-tCnt:, index:index+isoCnt] = 1
createOutput(path, model_type, args)
path.gamma[-tCnt:, index:index+isoCnt] = 0
path.count_increment()
# Initialize default values
path.reinit()
def add_newlinks_beta_to_beta(path, args):
""" function to add new links to above all of the betas """
# Initialize default values
path.reinit()
# Iterate overall of the endogenous variables (cols of beta)
for index, gene in enumerate(path.yvar):
# Determine if gene has multiple isoforms
isoCnt = utils.isoCount(gene)
# Iterate over the the endogenous varaibles (rows of beta) and add link
# one at a time.
for row, target in enumerate(path.yvar):
# A gene cannot regulate itself in SEMs
if row != index:
# Determine if gene has multiple isoforms
tCnt = utils.isoCount(target)
# Skip when one gene is already regulating the other
if path.beta[row, index] == 0 and path.beta[index, row] == 0:
model_type = "Adding Newlinks Beta to Beta:\n {0} -> {1}".format(gene, target)
path.beta[row:row+tCnt, index:index+isoCnt] = 1
createOutput(path, model_type, args)
path.beta[row:row+tCnt, index:index+isoCnt] = 0
path.count_increment()
def add_genes_bt_beta(path, newgene, args):
""" Iterate through beta matrix and add genes between two betas. """
# Initialize default values again
path.reinit()
# Expand gamma and beta to account for new isoforms
for iso in range(0, newgene.count):
path.expand_beta()
path.expand_gamma()
# Make copy of oiriginal for iterating
_yvar = list(path.yvar)
# Append new gene to yvar
path.yvar.append(newgene.name)
# Iterate through BETA matrix and add genes between betas
cIndex = 0
for col in _yvar:
# How many isoforms does the current column have
colCnt = utils.isoCount(col)
rIndex = 0
for row in _yvar:
# A gene cannot act on itself, so skip when row=col
if col == row:
continue
# How many isoforms does the current row have
rowCnt = utils.isoCount(row)
# Zero slice, is the current location in Beta, if there are 1s
# there I will turn them 0
zSlice = np.s_[rIndex:rIndex + rowCnt, cIndex:cIndex + colCnt]
# Target slice, is the bottom 'newgene.count' rows, that will get turned 1
# showing that current 'col' is acting on the newgene
tSlice = np.s_[-newgene.count:, cIndex:cIndex + colCnt]
# Source slice, is the right most 'newgene.count' cols, that will get turned 1
# showing that newgene is acting on the current row
sSlice = np.s_[rIndex:rIndex + rowCnt, -newgene.count:]
# If there was a previous interaction (i.e. 1) then make models
if np.all(path.beta[zSlice]):
model_type = "Adding Genes Between Betas:\n {0} -> {2} -> {1}".format(
col, row, newgene.name)
path.beta[zSlice] = 0
path.beta[tSlice] = 1
path.beta[sSlice] = 1
createOutput(path, model_type, args)
path.beta[zSlice] = 1
path.beta[tSlice] = 0
path.beta[sSlice] = 0
path.count_increment()
rIndex += rowCnt
cIndex += colCnt
def add_genes_bt_gamma_beta(path, newgene, args):
""" Iterate through gamma matrix and place new genes between gamma and ds beta. """
# Initialize default values again
path.reinit()
# Expand gamma and beta to account for new isoforms
for iso in range(0, newgene.count):
path.expand_beta()
path.expand_gamma()
# Make copy of oiriginal for iterating
_yvar = list(path.yvar)
# Add newGene to yvar because they are new endogenous genes
path.yvar.append(newgene.name)
# Iterate through each row of GAMMA matrix and place new gene in between
# gamma and beta
cIndex = 0
for col in path.xvar:
# How many isoforms does the current column have
colCnt = utils.isoCount(col)
rIndex = 0
for row in _yvar:
# How many isoforms does the current row have
rowCnt = utils.isoCount(row)
# Zero slice, is the current location in Gamma, if there are 1s
# there I will turn them 0
zSlice = np.s_[rIndex:rIndex + rowCnt, cIndex:cIndex + colCnt]
# Target slice, is the bottom 'newgene.count' rows in GAMMA, that
# will get turned 1 showing that current 'col' is acting on the
# newgene
tSlice = np.s_[-newgene.count:, cIndex:cIndex + colCnt]
# Source slice, is the right most 'newgene.count' cols in BETA,
# that will get turned 1 showing that newgene is acting on the
# current row
sSlice = np.s_[rIndex:rIndex + rowCnt, -newgene.count:]
# If there was a previous interaction (i.e. 1) then make models
if np.all(path.gamma[zSlice]):
model_type = "Adding Genes Between Gamma and Beta:\n {0} -> {2} -> {1}".format(
col, row, newgene.name)
path.gamma[zSlice] = 0
path.gamma[tSlice] = 1
path.beta[sSlice] = 1
createOutput(path, model_type, args)
path.gamma[zSlice] = 1
path.gamma[tSlice] = 0
path.beta[sSlice] = 0
path.count_increment()
rIndex += rowCnt
cIndex += colCnt
def add_genes_above_beta(path, newgene, args):
""" Iterate through gamma matrix and place new genes upstream of betas. """
# Initialize default values
path.reinit()
# Expand matrices GAMMA and PHI matrices
for iso in range(0, newgene.count):
path.expand_gamma(axis=1) # Add column not row
path.expand_phi()
# Append new gene to yvar
path.xvar.append(newgene.name)
index = 0
for gene in path.yvar:
# How many isoforms does the current gene have
isoCnt = utils.isoCount(gene)
# Create slice index of right most 'newgene.count' columns of gamma and
# alterating rows of gamma
gSlice = np.s_[index:index + isoCnt, -newgene.count:]
model_type = "Adding Genes Upstream of beta:\n {1} -> {0}".format(
gene, newgene.name)
path.gamma[gSlice] = 1
createOutput(path, model_type, args)
path.gamma[gSlice] = 0
path.count_increment()
index += isoCnt
def add_genes_ds_gamma(path, newgene, args):
""" Iterate through gamma matrix and place new genes downstream of each gamma. """
# Initialize default values
path.reinit()
# Expand gamma and beta to account for new isoforms
for iso in range(0, newgene.count):
path.expand_beta()
path.expand_gamma()
# Append new gene to yvar
path.yvar.append(newgene.name)
# Iterate through GAMMA matrix and output all possible models
index = 0
for gene in path.xvar:
# How many isoforms does the current gene have
isoCnt = utils.isoCount(gene)
# Create slice index of bottom 'newgene.count' rows of gamma and
# alternating columns of gamma
gSlice = np.s_[-newgene.count:, index:index + isoCnt]
model_type = "Adding Genes Downstream of Gamma:\n {0} -> {1}".format(
gene, newgene.name)
path.gamma[gSlice] = 1
createOutput(path, model_type, args)
path.gamma[gSlice] = 0
path.count_increment()
index += isoCnt
def buildGamma(yvar, xvar, paths):
""" Gamma has 'yvar' rows and 'xvar' columns. Gamma is a 0,1 matrix of the
relationships between exogenous (xvar) to endogenous (yvar) genes.
"""
# flatten yvar and xvar to remove isoform grouping
fyvar = utils.flatten_list(yvar, 1)
fxvar = utils.flatten_list(xvar, 1)
# Initialize Gamma as all 0's
nyvar = len(fyvar)
nxvar = len(fxvar)
Gamma = np.zeros((nyvar, nxvar))
# Iterate through xvar and see if there are any relationships
for colIndex, value in enumerate(xvar):
# If the current gene has a downstream target continue
if paths[value]:
# get isoform count
isoCnt = utils.isoCount(value)
# get flattened list of downstream targets
targets = utils.flatten_list(paths[value],1)
# Add ones to correct spot in Gamma
for target in targets:
rowIndex = fyvar.index(target)
Gamma[rowIndex, colIndex:colIndex+isoCnt] = 1
return Gamma
def buildBeta(yvar, paths):
""" Beta is a square matrix where the rows and columns are equal to the
endogenous genes. In other words, Beta is a 0,1 matrix of the relationships
between endogenous (yvar) genes.
"""
# flatten the yvar list to remove isoform grouping
fyvar = utils.flatten_list(yvar, 1)
# Initialize Beta as all 0's
nyvar = len(fyvar)
Beta = np.zeros((nyvar, nyvar))
# Iterate through yvar and see if there are any relationships
for colIndex, value in enumerate(yvar):
# If the current gene has a downstream target continue
if paths[value]:
# get isoform count
isoCnt = utils.isoCount(value)
# get flattened list of downstream targets
targets = utils.flatten_list(paths[value],1)
# Add ones to correct spot in Beta
for target in targets:
rowIndex = fyvar.index(target)
Beta[rowIndex, colIndex:colIndex+isoCnt] = 1
return Beta
def __init__(self, newGene):
try:
# Determine if the gene we are adding to the model has multiple isoforms
# and create a counter
self.name = newGene
self.count = utils.isoCount(self.name)
except ValueError:
logger.error(
"If you are adding genes you need to create a new gene object."
)
def add_newlinks_gamma_to_beta(path, args):
""" function to add new links to above all of the betas """
# Initialize default values
path.reinit()
# Iterate overall of the exogenous variables
for index, gene in enumerate(path.xvar):
# Determine if gene has multiple isoforms
isoCnt = utils.isoCount(gene)
# Iterate over the rows of gamma and add link one at a time. In other words,
# add new links to each of the endogenous variables.
for row, target in enumerate(path.yvar):
# Determine if gene has multiple isoforms
tCnt = utils.isoCount(target)
if path.gamma[row, index] == 0:
model_type = "Adding Newlinks Gamma to Beta:\n {0} -> {1}".format(gene, target)
path.gamma[row:row+tCnt, index:index+isoCnt] = 1
createOutput(path, model_type, args)
path.gamma[row:row+tCnt, index:index+isoCnt] = 0
path.count_increment()
def convert_ExogToEndog(self, gene):
""" Convert a gene/isoform from being exogenous to endogenous """
# Get gene location from xvar
xInd = self.xvar.index(gene)
# Remove the gene/isoform from the xvar list and add it to the yvar list
curr = self.xvar.pop(xInd)
self.yvar.append(curr)
# How many isoforms does this gene have
isoCnt = utils.isoCount(gene)
# Create a range of indices so that we can remove this gene from gamma
# and phi
matRange = range(xInd, xInd + isoCnt)
# Pull a list of causative effects from gamma, we need to transfer
# these to the beta matrix.
# NOTE: I am assuming that isoforms have same effects
effects = self.gamma[:, xInd]
# Delete the corresponding col from gamma and row and col from phi
self.del_col_gamma(matRange)
self.del_row_col_phi(matRange)
# Expand gamma and beta to account for new isoforms
for cnt in matRange:
self.expand_gamma()
self.expand_beta()
# Identify where the effects from gamma are equal to 1 and set those in
# beta
causeInd = np.where(np.array(effects) == 1)[0]
for ind2 in causeInd:
self.beta[ind2, -isoCnt:] = 1
# Set row/col counts
self.rc_count()
def add_genes_above_gamma(path, newgene, args):
""" Iterate through gamma matrix and place new genes upstream of gammas. """
# Initialize default values
path.reinit()
# Make copy of oiriginal for iterating
_xvar = list(path.xvar)
for gene in _xvar:
# How many isoforms does the current gene have
isoCnt = utils.isoCount(gene)
# Convert the current exogenous gene to an enogenous gene
path.convert_ExogToEndog(gene)
# Append new gene to xvar
path.xvar.append(newgene.name)
# Expand gamma and phi matrices for my new gene
for iso in range(0, newgene.count):
path.expand_gamma(axis=1) # Add column not row
path.expand_phi()
# Create slice index of right most 'newgene.count' columns of gamma and
# the bottom 'isoCnt' rows of gamma.
gSlice = np.s_[-isoCnt:, -newgene.count:]
model_type = "Adding Genes Upstream of Gamma:\n {1} -> {0}".format(
gene, newgene.name)
path.gamma[gSlice] = 1
createOutput(path, model_type, args)
path.count_increment()
# Reset I need to move different exogenous genes
path.reinit()
