pointer_pd['num_ctrl_spots_ft'] = [len(ctrl_pd_ft)] * len(pointer_pd)
data_log['num_ctrl_spots'] = len(ctrl_pd_ft)
print("### Image analysis: photobleaching correction ...")
# photobleaching factor calculation
if len(ctrl_pd_ft) != 0:
pointer_pd = ble.frap_pb_correction(pointer_pd, ctrl_pd_ft)
# normalize frap curve and measure mobile fraction and t-half based on curve itself
frap_pd = ble.frap_analysis(pointer_pd, max_t,
acquire_time_tseries, real_time,
frap_start_delay, frap_start_mode)
pointer_pd = pd.concat([pointer_pd, frap_pd], axis=1)
# frap curve fitting
print("### Imaging analysis: curve fitting ...")
pointer_pd = ble.frap_curve_fitting(pointer_pd)
pointer_pd['pos'] = [pos] * len(pointer_pd)
pointer_ft_pd = pointer_pd[pointer_pd['frap_filter_%s' %
fitting_mode] == 1]
data_log['num_frap_curves'] = [len(pointer_ft_pd)]
print("%d spots passed filters for FRAP curve quality control." %
data_log['num_frap_curves'][0])
# --------------------------
# OUTPUT
# --------------------------
print("### Export data ...")
storage_path = save_path
if not os.path.exists(storage_path):
os.makedirs(storage_path)
len(ctrl_nuclear_bg_pd_ft)
] * len(pointer_nuclear_bg_pd)
data_log['num_ctrl_spots_nuclear_bg'] = len(ctrl_nuclear_bg_pd_ft)
print("### Image analysis: photobleaching correction ...")
# photobleaching factor calculation
if len(ctrl_pd_ft) != 0:
pointer_pd = ble.frap_pb_correction(pointer_pd, ctrl_pd_ft)
# normalize frap curve and measure mobile fraction and t-half based on curve itself
frap_pd = ble.frap_analysis(pointer_pd, max_t, acquire_time_tseries,
real_time, frap_start_delay, frap_start_mode)
pointer_pd = pd.concat([pointer_pd, frap_pd], axis=1)
# frap curve fitting
print("### Imaging analysis: curve fitting ...")
pointer_pd = ble.frap_curve_fitting(pointer_pd)
pointer_pd['pos'] = [pos] * len(pointer_pd)
pointer_ft_pd = pointer_pd[pointer_pd['frap_filter_%s' %
fitting_mode] == 1]
data_log['num_frap_curves'] = [len(pointer_ft_pd)]
print("%d spots passed filters for FRAP curve quality control." %
data_log['num_frap_curves'][0])
# --------------------------
# OUTPUT
# --------------------------
print("### Export data ...")
storage_path = save_path
if not os.path.exists(storage_path):
os.makedirs(storage_path)