def import_dataset(params, name: str) -> SequenceDataset:
"""
Returns randomly generated receptor dataset according to the parameters;
YAML specification:
result_path: path/where/to/store/results/
sequence_count: 100 # number of random sequences to generate
chain_1_length_probabilities:
14: 0.8 # 80% of all generated sequences for all sequences will have length 14
15: 0.2 # 20% of all generated sequences across all sequences will have length 15
labels:
epitope1: # label name
True: 0.5 # 50% of the sequences will have class True
False: 0.5 # 50% of the sequences will have class False
epitope2: # next label with classes that will be assigned to sequences independently of the previous label or other parameters
1: 0.3 # 30% of the generated sequences will have class 1
0: 0.7 # 70% of the generated sequences will have class 0
"""
valid_keys = [
"sequence_count", "length_probabilities", "labels", "result_path"
]
ParameterValidator.assert_all_in_valid_list(
list(params.keys()), valid_keys, "RandomSequenceDatasetImport",
"params")
return RandomDatasetGenerator.generate_sequence_dataset(
sequence_count=params["sequence_count"],
length_probabilities=params["length_probabilities"],
labels=params["labels"],
path=params["result_path"])
def test_generate(self):
path = EnvironmentSettings.tmp_test_path + "relevant_sequence_exporter/"
PathBuilder.build(path)
df = pd.DataFrame({
"v_genes": ["TRBV1-1", "TRBV1-1"],
'j_genes': ["TRBJ1-1", "TRBJ1-2"],
"sequence_aas": ['ACCF', "EEFG"]
})
df.to_csv(path + 'sequences.csv', index=False)
dataset = RandomDatasetGenerator.generate_repertoire_dataset(
2, {2: 1}, {4: 1}, {}, path + "data/")
dataset.encoded_data = EncodedData(
examples=None,
info={'relevant_sequence_path': path + 'sequences.csv'},
encoding="SequenceAbundanceEncoder")
report_result = RelevantSequenceExporter(dataset, path + "result/",
'somename').generate_report()
self.assertEqual(1, len(report_result.output_tables))
self.assertTrue(os.path.isfile(report_result.output_tables[0].path))
self.assertTrue(
all(col in ["v_call", "j_call", "cdr3_aa"] for col in pd.read_csv(
report_result.output_tables[0].path).columns))
shutil.rmtree(path)
def test_run(self):
path = PathBuilder.build(
f"{EnvironmentSettings.tmp_test_path}subsampling/")
dataset = RandomDatasetGenerator.generate_receptor_dataset(
200,
labels={"epitope": {
"A": 0.5,
"B": 0.5
}},
path=path,
chain_1_length_probabilities={3: 1},
chain_2_length_probabilities={4: 1})
dataset.name = "d1"
inst = SubsamplingInstruction(dataset=dataset,
subsampled_dataset_sizes=[100, 50],
dataset_export_formats=[PickleExporter],
name="subsampling_inst")
state = inst.run(path + "result/")
self.assertEqual(2, len(state.subsampled_datasets))
self.assertEqual(100, state.subsampled_datasets[0].get_example_count())
self.assertEqual(50, state.subsampled_datasets[1].get_example_count())
self.assertTrue(
all(
os.path.isfile(state.subsampled_dataset_paths[name]['pickle'])
for name in
[dataset.name for dataset in state.subsampled_datasets]))
shutil.rmtree(path)
def test_generate(self):
path = PathBuilder.build(f"{EnvironmentSettings.tmp_test_path}kernel_sequence_logo/")
dataset = RandomDatasetGenerator.generate_receptor_dataset(receptor_count=500, chain_1_length_probabilities={4: 1},
chain_2_length_probabilities={4: 1},
labels={"CMV": {True: 0.5, False: 0.5}}, path=path + "dataset/")
enc_dataset = OneHotReceptorEncoder(True, 1, False, "enc1").encode(dataset, EncoderParams(path + "result/",
LabelConfiguration([Label("CMV", [True, False])])))
cnn = ReceptorCNN(kernel_count=2, kernel_size=[3], positional_channels=3, sequence_type="amino_acid", device="cpu",
number_of_threads=4, random_seed=1, learning_rate=0.01, iteration_count=10, l1_weight_decay=0.1, evaluate_at=5,
batch_size=100, training_percentage=0.8, l2_weight_decay=0.0)
cnn.fit(enc_dataset.encoded_data, "CMV")
report = KernelSequenceLogo(method=cnn, result_path=path + "logos/")
report.generate_report()
self.assertTrue(os.path.isfile(f"{path}logos/alpha_kernel_3_1.png"))
self.assertTrue(os.path.isfile(f"{path}logos/alpha_kernel_3_2.png"))
self.assertTrue(os.path.isfile(f"{path}logos/beta_kernel_3_1.png"))
self.assertTrue(os.path.isfile(f"{path}logos/beta_kernel_3_2.png"))
self.assertTrue(os.path.isfile(f"{path}logos/alpha_kernel_3_1.csv"))
self.assertTrue(os.path.isfile(f"{path}logos/alpha_kernel_3_2.csv"))
self.assertTrue(os.path.isfile(f"{path}logos/beta_kernel_3_1.csv"))
self.assertTrue(os.path.isfile(f"{path}logos/beta_kernel_3_2.csv"))
self.assertTrue(os.path.isfile(f"{path}logos/fully_connected_layer_weights.csv"))
self.assertTrue(os.path.isfile(f"{path}logos/fully_connected_layer_weights.html"))
shutil.rmtree(path)
def test_run(self):
path = PathBuilder.build(
f"{EnvironmentSettings.tmp_test_path}dataset_export_instruction/")
dataset = RandomDatasetGenerator.generate_repertoire_dataset(
10, {10: 1}, {12: 1}, {}, path)
dataset.name = "d1"
instruction = DatasetExportInstruction(datasets=[dataset],
exporters=[AIRRExporter])
result_path = f"{path}generated/"
state = instruction.run(result_path=result_path)
self.assertTrue(isinstance(state, DatasetExportState))
self.assertEqual(1, len(state.datasets))
self.assertEqual(1, len(state.formats))
self.assertEqual("AIRR", state.formats[0])
self.assertTrue(os.path.isdir(result_path))
self.assertEqual(1, len(list(glob(f"{state.result_path}*/"))))
self.assertEqual(
1, len(list(glob(f"{state.result_path}{dataset.name}/*/"))))
self.assertTrue(
os.path.isdir(f"{state.result_path}{dataset.name}/AIRR/"))
self.assertTrue(
os.path.isfile(
f"{state.result_path}{dataset.name}/AIRR/metadata.csv"))
self.assertEqual(
10,
len(
list(
glob(
f"{state.result_path}{dataset.name}/AIRR/repertoires/*"
))))
shutil.rmtree(path)
def test__split_repertoire_dataset(self):
path = PathBuilder.build(EnvironmentSettings.tmp_test_path + "manual_splitter/")
dataset = RandomDatasetGenerator.generate_repertoire_dataset(10, {4: 1}, {3: 1}, {}, path)
train_metadata = pd.DataFrame({"subject_id": ["rep_1", "rep_2", "rep_4", "rep_5", "rep_9", "rep_7"]})
train_metadata.to_csv(path + "train.csv")
test_metadata = pd.DataFrame({"subject_id": ["rep_0", "rep_3", "rep_6", "rep_8"]})
test_metadata.to_csv(path + "test.csv")
train_datasets, test_datasets = ManualSplitter._split_repertoire_dataset(
DataSplitterParams(dataset, SplitType.MANUAL, split_count=1, paths=[path + 'result/'],
split_config=SplitConfig(manual_config=ManualSplitConfig(path + "train.csv",
path + "test.csv"),
split_count=1, split_strategy=SplitType.MANUAL)))
self.assertEqual(1, len(train_datasets))
self.assertEqual(1, len(test_datasets))
self.assertEqual(6, train_datasets[0].get_example_count())
self.assertEqual(4, test_datasets[0].get_example_count())
self.assertTrue(all(subject_id in ["rep_1", "rep_2", "rep_4", "rep_5", "rep_9", "rep_7"]
for subject_id in train_datasets[0].get_metadata(["subject_id"])["subject_id"]))
self.assertTrue(all(subject_id in ["rep_0", "rep_3", "rep_6", "rep_8"]
for subject_id in test_datasets[0].get_metadata(["subject_id"])["subject_id"]))
self.assertTrue(os.path.isfile(train_datasets[0].metadata_file))
self.assertTrue(os.path.isfile(test_datasets[0].metadata_file))
shutil.rmtree(path)
def import_dataset(params: dict, dataset_name: str) -> RepertoireDataset:
valid_keys = ["result_path", "repertoire_count", "sequence_count_probabilities", "sequence_length_probabilities", "labels"]
ParameterValidator.assert_all_in_valid_list(list(params.keys()), valid_keys, "RandomRepertoireDatasetImport", "params")
return RandomDatasetGenerator.generate_repertoire_dataset(repertoire_count=params["repertoire_count"],
sequence_count_probabilities=params["sequence_count_probabilities"],
sequence_length_probabilities=params["sequence_length_probabilities"],
labels=params["labels"],
path=params["result_path"])
def test_run(self):
path = EnvironmentSettings.tmp_test_path + "mlapplicationtest/"
PathBuilder.build(path)
dataset = RandomDatasetGenerator.generate_repertoire_dataset(
50, {5: 1}, {5: 1}, {"l1": {
1: 0.5,
2: 0.5
}}, path + 'dataset/')
ml_method = LogisticRegression()
encoder = KmerFreqRepertoireEncoder(
NormalizationType.RELATIVE_FREQUENCY,
ReadsType.UNIQUE,
SequenceEncodingType.CONTINUOUS_KMER,
3,
scale_to_zero_mean=True,
scale_to_unit_variance=True)
label_config = LabelConfiguration([Label("l1", [1, 2])])
enc_dataset = encoder.encode(
dataset,
EncoderParams(result_path=path,
label_config=label_config,
filename="tmp_enc_dataset.pickle",
pool_size=4))
ml_method.fit(enc_dataset.encoded_data, 'l1')
hp_setting = HPSetting(
encoder, {
"normalization_type": "relative_frequency",
"reads": "unique",
"sequence_encoding": "continuous_kmer",
"k": 3,
"scale_to_zero_mean": True,
"scale_to_unit_variance": True
}, ml_method, {}, [], 'enc1', 'ml1')
PathBuilder.build(path + 'result/instr1/')
shutil.copy(path + 'dict_vectorizer.pickle',
path + 'result/instr1/dict_vectorizer.pickle')
shutil.copy(path + 'scaler.pickle',
path + 'result/instr1/scaler.pickle')
ml_app = MLApplicationInstruction(dataset, label_config, hp_setting, 4,
"instr1", False)
ml_app.run(path + 'result/')
predictions_path = path + "result/instr1/predictions.csv"
self.assertTrue(os.path.isfile(predictions_path))
df = pd.read_csv(predictions_path)
self.assertEqual(50, df.shape[0])
shutil.rmtree(path)
def test_encode(self):
path = PathBuilder.build(EnvironmentSettings.tmp_test_path + "atchley_kmer_encoding/")
dataset = RandomDatasetGenerator.generate_repertoire_dataset(3, {1: 1}, {4: 1}, {"l1": {True: 0.4, False: 0.6}}, path + "dataset/")
encoder = AtchleyKmerEncoder.build_object(dataset, **{"k": 2, "skip_first_n_aa": 1, "skip_last_n_aa": 1, "abundance": "RELATIVE_ABUNDANCE",
"normalize_all_features": False})
encoded_dataset = encoder.encode(dataset, EncoderParams(path + "result/", LabelConfiguration(labels=[Label("l1")])))
self.assertEqual((3, 11, 3), encoded_dataset.encoded_data.examples.shape)
self.assertEqual(0., encoded_dataset.encoded_data.examples[0, -1, 0])
shutil.rmtree(path)
def test_export_receptor_dataset(self):
path = EnvironmentSettings.tmp_test_path + "pickleexporter_receptor/"
PathBuilder.build(path)
dataset = RandomDatasetGenerator.generate_receptor_dataset(
10, {2: 1}, {3: 1}, {}, path)
dataset.name = "d1"
PickleExporter.export(dataset, path)
with open(f"{path}/{dataset.name}.iml_dataset", "rb") as file:
dataset2 = pickle.load(file)
self.assertTrue(isinstance(dataset2, ReceptorDataset))
self.assertEqual(10, dataset2.get_example_count())
shutil.rmtree(path)
def test_generate_sequence_dataset(self):
path = f"{EnvironmentSettings.tmp_test_path}random_sequence_dataset_generation/"
dataset = RandomDatasetGenerator.generate_sequence_dataset(sequence_count=100,
length_probabilities={4: 0.5, 5: 0.5},
labels={"HLA": {"A": 0.5, "B": 0.5}},
path=path)
self.assertEqual(SequenceDataset, type(dataset))
self.assertEqual(100, dataset.get_example_count())
for sequence in dataset.get_data():
self.assertTrue(len(sequence.amino_acid_sequence) in [4, 5])
self.assertTrue(sequence.get_attribute("HLA") in ["A", "B"])
shutil.rmtree(path)
def test_parse(self):
path = PathBuilder.build(
f'{EnvironmentSettings.tmp_test_path}subsampling_parser/')
dataset = RandomDatasetGenerator.generate_receptor_dataset(
30, {3: 1}, {2: 1}, {}, path)
symbol_table = SymbolTable()
symbol_table.add("d1", SymbolType.DATASET, dataset)
SubsamplingParser().parse(
'inst1', {
'dataset': 'd1',
'type': 'Subsampling',
'subsampled_dataset_sizes': [10, 20],
'dataset_export_formats': ['Pickle']
}, symbol_table)
with self.assertRaises(AssertionError):
SubsamplingParser().parse(
'inst1', {
'dataset': 'd1',
'type': 'Subsampling',
'subsampled_dataset_sizes': [10, 50],
'dataset_export_formats': ['Pickle']
}, symbol_table)
with self.assertRaises(AssertionError):
SubsamplingParser().parse(
'inst1', {
'dataset': 'd2',
'type': 'Subsampling',
'subsampled_dataset_sizes': [10, 20],
'dataset_export_formats': ['Pickle']
}, symbol_table)
with self.assertRaises(AssertionError):
SubsamplingParser().parse(
'inst1', {
'dataset': 'd2',
'type': 'Subsampling',
'subsampled_dataset_sizes': [10, 20],
'dataset_export_formats': ['Random']
}, symbol_table)
shutil.rmtree(path)
def test_generate_receptor_dataset(self):
path = f"{EnvironmentSettings.tmp_test_path}random_receptor_dataset_generation/"
dataset = RandomDatasetGenerator.generate_receptor_dataset(receptor_count=100,
chain_1_length_probabilities={4: 0.5, 5: 0.5},
chain_2_length_probabilities={4: 0.5, 5: 0.5},
labels={"HLA": {"A": 0.5, "B": 0.5}},
path=path)
self.assertEqual(ReceptorDataset, type(dataset))
self.assertEqual(100, dataset.get_example_count())
for receptor in dataset.get_data():
self.assertTrue(len(sequence_aa) in [4, 5] for sequence_aa in [receptor.alpha.amino_acid_sequence, receptor.beta.amino_acid_sequence])
self.assertTrue(receptor.metadata["HLA"] in ["A", "B"])
shutil.rmtree(path)
def test_generate_repertoire_dataset(self):
path = f"{EnvironmentSettings.tmp_test_path}random_repertoire_dataset_generation/"
dataset = RandomDatasetGenerator.generate_repertoire_dataset(repertoire_count=100,
sequence_count_probabilities={5: 0.5, 6: 0.5},
sequence_length_probabilities={4: 0.5, 5: 0.5},
labels={"HLA": {"A": 0.5, "B": 0.5}},
path=path)
self.assertEqual(RepertoireDataset, type(dataset))
self.assertEqual(100, dataset.get_example_count())
for repertoire in dataset.repertoires:
self.assertTrue(repertoire.get_element_count() == 5 or repertoire.get_element_count() == 6)
self.assertTrue(all(len(sequence_aa) in [4, 5] for sequence_aa in repertoire.get_sequence_aas().tolist()))
self.assertTrue(repertoire.metadata["HLA"] in ["A", "B"])
shutil.rmtree(path)
def test_run_with_receptors(self):
path = PathBuilder.build(EnvironmentSettings.root_path + "test/tmp/signalImplanter_receptor/")
dataset = RandomDatasetGenerator.generate_receptor_dataset(100, {10: 1}, {12: 1}, {}, path + "dataset/")
motif1 = Motif(identifier="motif1", instantiation=GappedKmerInstantiation(), seed_chain1="AAA", name_chain1=Chain.ALPHA, seed_chain2="CCC",
name_chain2=Chain.BETA)
signal1 = Signal(identifier="signal1", motifs=[motif1], implanting_strategy=ReceptorImplanting(GappedMotifImplanting()))
simulation = Simulation([Implanting(dataset_implanting_rate=0.5, signals=[signal1])])
sim_state = SimulationState(dataset=dataset, result_path=path, simulation=simulation, signals=[signal1])
new_dataset = SignalImplanter.run(sim_state)
self.assertEqual(100, new_dataset.get_example_count())
self.assertEqual(50, len([receptor for receptor in new_dataset.get_data(40) if receptor.metadata["signal_signal1"] is True]))
shutil.rmtree(path)
def test_generate(self):
path = PathBuilder.build(EnvironmentSettings.tmp_test_path +
"gliph2_export/")
dataset = RandomDatasetGenerator.generate_receptor_dataset(
10, {3: 1}, {2: 1}, {"epitope": {
"ep1": 0.4,
"ep2": 0.6
}}, path)
report_result = GLIPH2Exporter(dataset, path + "result/", "somename",
"epitope").generate_report()
self.assertEqual(1, len(report_result.output_tables))
self.assertTrue(os.path.isfile(report_result.output_tables[0].path))
df = pd.read_csv(report_result.output_tables[0].path, sep="\t")
self.assertTrue(
all(col in [
"CDR3b", "TRBV", "TRBJ", "CDR3a", "subject:condition", "count"
] for col in df.columns))
self.assertEqual(10, df.shape[0])
shutil.rmtree(path)
def test_fit(self):
path = PathBuilder.build(EnvironmentSettings.tmp_test_path +
"kmermil/")
repertoire_count = 10
dataset = RandomDatasetGenerator.generate_repertoire_dataset(
repertoire_count=repertoire_count,
sequence_count_probabilities={2: 1},
sequence_length_probabilities={4: 1},
labels={"l1": {
True: 0.5,
False: 0.5
}},
path=path + "dataset/")
enc_dataset = AtchleyKmerEncoder(
2, 1, 1, 'relative_abundance', False).encode(
dataset,
EncoderParams(path + "result/",
LabelConfiguration([Label("l1",
[True, False])])))
cls = AtchleyKmerMILClassifier(iteration_count=10,
threshold=-0.0001,
evaluate_at=2,
use_early_stopping=False,
random_seed=1,
learning_rate=0.01,
zero_abundance_weight_init=True,
number_of_threads=8)
cls.fit(enc_dataset.encoded_data, "l1")
predictions = cls.predict(enc_dataset.encoded_data, "l1")
self.assertEqual(repertoire_count, len(predictions["l1"]))
self.assertEqual(
repertoire_count,
len([pred for pred in predictions["l1"]
if isinstance(pred, bool)]))
predictions_proba = cls.predict_proba(enc_dataset.encoded_data, "l1")
self.assertEqual(repertoire_count,
np.rint(np.sum(predictions_proba["l1"])))
self.assertEqual(repertoire_count, predictions_proba["l1"].shape[0])
cls.store(path + "model_storage/",
feature_names=enc_dataset.encoded_data.feature_names)
cls2 = AtchleyKmerMILClassifier(iteration_count=10,
threshold=-0.0001,
evaluate_at=2,
use_early_stopping=False,
random_seed=1,
learning_rate=0.01,
zero_abundance_weight_init=True,
number_of_threads=8)
cls2.load(path + "model_storage/")
cls2_vars = vars(cls2)
del cls2_vars["logistic_regression"]
cls_vars = vars(cls)
del cls_vars["logistic_regression"]
for item, value in cls_vars.items():
if not isinstance(value, np.ndarray):
loaded_value = cls2_vars[item]
self.assertEqual(value, loaded_value)
model = cls.get_model("l1")
self.assertEqual(vars(cls), model)
shutil.rmtree(path)
def test_fit(self):
path = PathBuilder.build(EnvironmentSettings.tmp_test_path + "cnn/")
dataset = RandomDatasetGenerator.generate_receptor_dataset(
receptor_count=500,
chain_1_length_probabilities={4: 1},
chain_2_length_probabilities={4: 1},
labels={"CMV": {
True: 0.5,
False: 0.5
}},
path=path + "dataset/")
enc_dataset = OneHotReceptorEncoder(True, 1, False, "enc1").encode(
dataset,
EncoderParams(path + "result/",
LabelConfiguration([Label("CMV", [True, False])])))
cnn = ReceptorCNN(kernel_count=2,
kernel_size=[3],
positional_channels=3,
sequence_type="amino_acid",
device="cpu",
number_of_threads=4,
random_seed=1,
learning_rate=0.01,
iteration_count=10,
l1_weight_decay=0.1,
evaluate_at=5,
batch_size=100,
training_percentage=0.8,
l2_weight_decay=0.0)
cnn.fit(encoded_data=enc_dataset.encoded_data, label_name="CMV")
predictions = cnn.predict(enc_dataset.encoded_data, "CMV")
self.assertEqual(500, len(predictions["CMV"]))
self.assertEqual(
500,
len([
pred for pred in predictions["CMV"] if isinstance(pred, bool)
]))
predictions_proba = cnn.predict_proba(enc_dataset.encoded_data, "CMV")
self.assertEqual(500, np.rint(np.sum(predictions_proba["CMV"])))
self.assertEqual(500, predictions_proba["CMV"].shape[0])
cnn.store(path + "model_storage/")
cnn2 = ReceptorCNN(sequence_type="amino_acid")
cnn2.load(path + "model_storage/")
cnn2_vars = vars(cnn2)
del cnn2_vars["CNN"]
cnn_vars = vars(cnn)
del cnn_vars["CNN"]
for item, value in cnn_vars.items():
if not isinstance(value, np.ndarray):
self.assertEqual(value, cnn2_vars[item])
model = cnn.get_model(["CMV"])
self.assertEqual(vars(cnn), model)
shutil.rmtree(path)
def test_run(self):
path = PathBuilder.build(f"{EnvironmentSettings.tmp_test_path}api_galaxy_yaml_tool/")
result_path = f"{path}result/"
dataset = RandomDatasetGenerator.generate_repertoire_dataset(10, {10: 1}, {12: 1}, {}, result_path)
dataset.name = "d1"
PickleExporter.export(dataset, result_path)
specs = {
"definitions": {
"datasets": {
"new_d1": {
"format": "Pickle",
"params": {
"metadata_file": f"{result_path}d1_metadata.csv"
}
},
"d2": {
"format": "RandomRepertoireDataset",
"params": {
"repertoire_count": 50,
"sequence_length_probabilities": {10: 1},
'sequence_count_probabilities': {10: 1},
'labels': {
"CD": {
True: 0.5,
False: 0.5
}
}
}
}
},
"encodings": {
"e1": {
"Word2Vec": {
"k": 3,
"model_type": "sequence",
"vector_size": 8,
}
},
"e2": {
"Word2Vec": {
"k": 3,
"model_type": "sequence",
"vector_size": 10,
}
},
},
"ml_methods": {
"simpleLR": {
"LogisticRegression": {
"penalty": "l1"
},
"model_selection_cv": False,
"model_selection_n_folds": -1,
}
},
},
"instructions": {
"inst1": {
"type": "DatasetExport",
"datasets": ["new_d1", 'd2'],
"export_formats": ["AIRR"]
},
"inst2": {
"type": "TrainMLModel",
"settings": [
{
"encoding": "e1",
"ml_method": "simpleLR"
},
{
"encoding": "e2",
"ml_method": "simpleLR"
}
],
"assessment": {
"split_strategy": "random",
"split_count": 1,
"training_percentage": 0.7
},
"selection": {
"split_strategy": "random",
"split_count": 2,
"training_percentage": 0.7
},
"labels": ["CD"],
"dataset": "d2",
"strategy": "GridSearch",
"metrics": ["accuracy", "auc"],
"reports": [],
"number_of_processes": 10,
"optimization_metric": "accuracy",
'refit_optimal_model': False,
"store_encoded_data": False
}
}
}
specs_path = f"{path}specs.yaml"
with open(specs_path, "w") as file:
yaml.dump(specs, file)
run_immuneML(Namespace(**{"specification_path": specs_path, "result_path": result_path + 'result/', 'tool': "GalaxyYamlTool"}))
self.assertTrue(os.path.exists(f"{result_path}result/inst1/new_d1/AIRR"))
self.assertTrue(os.path.exists(f"{result_path}result/inst1/d2/AIRR"))
self.assertTrue(os.path.exists(f"{result_path}result/d2"))
shutil.rmtree(path)