def test_non_overlap_true11(self):
"""
If everything works with new overlap parameter off
For specified input we expect not to find any overlap
:return:
"""
qclm_data = generate_qclm_input(11)
qclm_data.config.non_overlapping_primers = True
exp = set()
for mut in qclm_data.mutations:
exp.update(set(extract_position_from_key(mut)))
random.seed(121)
result = qclm_solve(qclm_data)
print(result)
mut_positions, primers_postions = extract_muations(result)
# determine overlaps
cnt_overlaps = count_overlaps(primers_postions)
print("The solution has {} number of overlapping primers".format(cnt_overlaps))
if len(mut_positions) != len(exp):
print(len(mut_positions), len(exp))
# we should have 0 overlaps
self.assertEqual(cnt_overlaps, 0)
def test_non_overlap_false(self):
"""
If everything works with new overlap parameter off
For specified input we expect to find some overlaps
:return:
"""
qclm_data = generate_qclm_input(11)
qclm_data.config.non_overlapping_primers = False
exp = set()
for mut in qclm_data.mutations:
exp.update(set(extract_position_from_key(mut)))
random.seed(121)
result = qclm_solve(qclm_data)
print(result)
mut_positions = set()
# here we store primers start and enc positions, as a key we use mutations, which the primer is covering
primers_postions = {}
for res in result["results"]:
# we create key from just mutation positions therefore [1:-1]
key = "-".join(res["mutations"])
key_pos = "-".join([m[1:-1] for m in res["mutations"]])
print(key, res["primers"])
for primer in res["primers"]:
st = int(primer["start"])
end = int(primer["start"]) + int(primer["length"])
# we can have multiple primers for 1 mutaiton set, therefore we store just the minimal start and maximal end
if key_pos in primers_postions.keys():
st = min(st, primers_postions[key_pos][0])
end = max(end, primers_postions[key_pos][1])
primers_postions[key_pos] = (st, end)
mut_positions.update(set(extract_position_from_key(key)))
# determine overlaps
cnt_overlaps = 0
for pair in primers_postions.values():
# we look if we have overlap with any other primer
if any([other[0] > pair[0] < other[1] for other in primers_postions.values()]):
cnt_overlaps += 1
print("The solution has {} number of overlapping primers".format(cnt_overlaps))
if len(mut_positions) != len(exp):
print(len(mut_positions), len(exp))
# we should have 0 overlaps
self.assertEqual(len(mut_positions), len(exp))
def runTest(self, non_overlap_flag, ind):
self.non_overlap_flag = non_overlap_flag
self.qclm_data = generate_qclm_input(ind)
self.qclm_data.config.non_overlapping_primers = self.non_overlap_flag
random.seed(121)
self.qclm_result = qclm_solve(self.qclm_data)
self.exp = set()
for mut in self.qclm_data.mutations:
self.exp.update(set(extract_position_from_key(mut)))
mut_positions = set()
# here we store primers start and enc positions, as a key we use mutations, which the primer is covering
primers_postions = {}
for res in self.qclm_result["results"]:
# we create key from just mutation positions therefore [1:-1]
key = "-".join(res["mutations"])
key_pos = "-".join([m[1:-1] for m in res["mutations"]])
print(key, res["primers"])
for primer in res["primers"]:
st = int(primer["start"])
end = int(primer["start"]) + int(primer["length"])
# we can have multiple primers for 1 mutaiton set, therefore we store just the minimal start and maximal end
if key_pos in primers_postions.keys():
st = min(st, primers_postions[key_pos][0])
end = max(end, primers_postions[key_pos][1])
primers_postions[key_pos] = (st, end)
mut_positions.update(set(extract_position_from_key(key)))
# determine overlaps
cnt_overlaps = 0
for pair in primers_postions.values():
# we look if we have overlap with any other primer
if any([other[0] > pair[0] < other[1] for other in primers_postions.values()]):
cnt_overlaps += 1
print("The solution has {} number of overlapping primers".format(cnt_overlaps))
if len(mut_positions) != len(self.xp):
print(len(mut_positions), len(self.exp))
# we should have 0 overlaps if the flag is on
if self.non_overlap_flag:
self.assertEqual(cnt_overlaps, 0)
if len(mut_positions) != len(self.exp):
print("Not full coverage ERROR: {}".format(str(len(mut_positions)/len(self.exp))))
