def create_feature_annot(loc_range, featuretype, s):
""" Create a new feature annotation at loc_range with featuretype on strand s. """
location = SeqFeature.FeatureLocation(
SeqFeature.ExactPosition(loc_range[0]),
SeqFeature.ExactPosition(loc_range[1]))
new_feature = SeqFeature.SeqFeature(location, type=featuretype, strand=s)
return (new_feature)
def modify_genbank(gb_file, fasta_file):
gb_filename = re.search(r'(.*/users/.*/uploads/.*).(\w*)', gb_file)
out_file = str(gb_filename.group(1)) + '_modified.' + str(
gb_filename.group(2))
genome = SeqIO.read(fasta_file, "fasta").seq
final_annotations = get_final_annotations(genome)
final_features = []
for record in SeqIO.parse(open(gb_file, "r"), "genbank"):
for feature in record.features:
if feature.type == "gene" or feature.type == "CDS":
locus_tag = feature.qualifiers["locus_tag"][0]
if locus_tag in final_annotations.keys():
new_start = final_annotations[locus_tag]["start"]
feature.location = SeqFeature.FeatureLocation(
SeqFeature.ExactPosition(new_start - 1),
SeqFeature.ExactPosition(
feature.location.end.position),
feature.location.strand)
if feature.type == "CDS":
feature.qualifiers["product"][0] = final_annotations[
locus_tag]["function"]
feature.qualifiers["translation"][
0] = final_annotations[locus_tag]["translation"]
else:
continue
final_features.append(feature) # Append final features
record.features = final_features
with open(out_file, "w") as new_gb:
SeqIO.write(record, new_gb, "genbank")
return out_file
def convert_annotations(block, gb):
# Add My annotations as features
for annotation in block["sequence"]["annotations"]:
gb_annot = SeqFeature.SeqFeature()
annotation_type = "unknown"
if "role" in annotation and annotation["role"] != "":
annotation_type = annotation["role"]
for key, value in annotation.iteritems():
if key not in ["start", "end", "notes", "strand", "color", "role"]:
gb_annot.qualifiers[key] = value
elif key == "color":
gb_annot.qualifiers["GC_Color"] = value
elif key == "notes":
for notes_key, notes_value in annotation["notes"].iteritems():
if notes_key == "genbank":
for gb_key, gb_value in notes_value.iteritems():
if gb_key not in ["type"]:
gb_annot.qualifiers[gb_key] = gb_value
elif gb_key == "type":
annotation_type = gb_value
if "start" in annotation:
strand = 1
if "strand" in annotation and annotation["strand"] == -1:
strand = -1
gb_annot.location = SeqFeature.FeatureLocation(
annotation["start"], annotation["end"] + 1, strand)
gb_annot.type = annotation_type
gb.features.append(gb_annot)
def read_reference(fname, genemap):
try:
ref = str(SeqIO.read(fname, 'fasta').seq)
except:
with open(fname, 'r') as fh:
ref = "".join([x.strip() for x in fh])
translations = {}
with open(genemap, 'r') as fh:
for line in fh:
if line[0] == '#':
continue
entries = [x.strip() for x in line.strip().split('\t')]
start = int(entries[3])
end = int(entries[4])
strand = entries[6]
attributes = {
x.split()[0]: ' '.join(x.split()[1:])
for x in entries[8].split(';')
}
if 'gene_name' in attributes:
name = attributes['gene_name'].strip('"')
else:
name = None
translation = Seq.translate(
SeqFeature.SeqFeature(
SeqFeature.FeatureLocation(
start - 1, end,
strand=-1 if strand == '-' else 1)).extract(ref))
translations[name] = str(translation)
return {"nuc": ref, "translations": translations}
def create_feature(hit, end):
'''
Creates a feature from the hit, setting colours
based on orientation and noting whether it is a
left or right end hit.
'''
# Set up coordinates
start = int(hit[1])
stop = int(hit[2])
quals = {}
location = SeqFeature.FeatureLocation(start, stop)
# Annotate with end information and colour accordingly
if end == 'five':
quals['colour'] = '2'
quals['end'] = 'left_end'
feat_type = 'left end'
elif end == 'three':
quals['colour'] = '7'
quals['end'] = 'right end'
feat_type = 'right_end'
# Create feature
feature = SeqFeature.SeqFeature(location, type=feat_type, qualifiers=quals)
return feature
def _parse_feature(element):
feature = SeqFeature.SeqFeature()
for k, v in element.attrib.items():
feature.qualifiers[k] = v
feature.type = element.attrib.get('type', '')
if 'id' in element.attrib:
feature.id = element.attrib['id']
for feature_element in element.getchildren():
if feature_element.tag == NS + 'location':
position_elements = feature_element.findall(NS +
'position')
if position_elements:
element = position_elements[0]
start_position = _parse_position(element, -1)
end_position = _parse_position(element)
else:
element = feature_element.findall(NS + 'begin')[0]
start_position = _parse_position(element, -1)
element = feature_element.findall(NS + 'end')[0]
end_position = _parse_position(element)
feature.location = SeqFeature.FeatureLocation(
start_position, end_position)
else:
try:
feature.qualifiers[feature_element.tag.replace(
NS, '')] = feature_element.text
except:
pass #skip unparsable tag
self.ParsedSeqRecord.features.append(feature)
def add_feature(self, search_str, label):
"""Label a feature by literal string match, failing silently.
Does not label features that wrap around circular sequence
Args:
search_str (str): string representing feature
label (str): feature name to display"""
f_matches = re.finditer(search_str, str(self.seq), re.IGNORECASE)
r_matches = ()
if search_str.lower() != dna.revc(search_str.lower()):
# Don't label palindromes twice
r_matches = re.finditer(dna.revc(search_str), str(self.seq),
re.IGNORECASE)
for m, strand in chain(izip_longest(f_matches, [1]),
izip_longest(r_matches, [-1])):
if m:
feature = SeqFeature.SeqFeature(
SeqFeature.FeatureLocation(m.start(), m.end(), strand),
'misc_feature')
color = self.colors.next()
feature.qualifiers = {
'label': [label],
'ApEinfo_fwdcolor': [color],
'ApEinfo_revcolor': [color],
'ApEinfo_graphicformat':
['arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0']
}
self.features.append(feature)
return
def _parse_feature(element):
feature = SeqFeature.SeqFeature()
for k, v in element.attrib.items():
feature.qualifiers[k] = v
feature.type = element.attrib.get("type", "")
if "id" in element.attrib:
feature.id = element.attrib["id"]
for feature_element in element:
if feature_element.tag == NS + "location":
position_elements = feature_element.findall(NS + "position")
if position_elements:
element = position_elements[0]
start_position = _parse_position(element, -1)
end_position = _parse_position(element)
else:
element = feature_element.findall(NS + "begin")[0]
start_position = _parse_position(element, -1)
element = feature_element.findall(NS + "end")[0]
end_position = _parse_position(element)
feature.location = SeqFeature.FeatureLocation(
start_position, end_position
)
else:
try:
feature.qualifiers[
feature_element.tag.replace(NS, "")
] = feature_element.text
except Exception: # TODO - Which exceptions?
pass # skip unparsable tag
self.ParsedSeqRecord.features.append(feature)
def _retrieve_reference(adaptor, primary_id):
# XXX dbxref_qualifier_value
refs = adaptor.execute_and_fetchall(
"SELECT start_pos, end_pos, "
" location, title, authors,"
" dbname, accession"
" FROM bioentry_reference"
" JOIN reference USING (reference_id)"
" LEFT JOIN dbxref USING (dbxref_id)"
" WHERE bioentry_id = %s"
" ORDER BY rank", (primary_id,))
references = []
for start, end, location, title, authors, dbname, accession in refs:
reference = SeqFeature.Reference()
# If the start/end are missing, reference.location is an empty list
if (start is not None) or (end is not None):
if start is not None:
start -= 1 # python counting
reference.location = [SeqFeature.FeatureLocation(start, end)]
# Don't replace the default "" with None.
if authors:
reference.authors = authors
if title:
reference.title = title
reference.journal = location
if dbname == 'PUBMED':
reference.pubmed_id = accession
elif dbname == 'MEDLINE':
reference.medline_id = accession
references.append(reference)
if references:
return {'references': references}
else:
return {}
def make_genbank_recs(rec):
new_rec = rec
#new_rec.seq.alphabet = generic_dna
scaffold = new_rec.id
scaffold_recs = list(
filter(lambda x: x.id.startswith(scaffold + '_'), protein_recs))
for protein_rec in scaffold_recs:
start = int(protein_rec.description.split(' # ')[1])
startpos = SeqFeature.ExactPosition(start)
end = int(protein_rec.description.split(' # ')[2])
endpos = int(SeqFeature.ExactPosition(end))
strand = int(protein_rec.description.split(' # ')[3])
rec_location = FeatureLocation(startpos, endpos)
rec_feature = SeqFeature.SeqFeature(rec_location,
type="CDS",
strand=strand)
#Add ORF name without genome ID
rec_feature.qualifiers['protein_id'] = protein_rec.id
rec_feature.qualifiers['translation'] = protein_rec.seq
rec_feature.qualifiers['locus_tag'] = protein_rec.description
new_rec.features.append(rec_feature)
return new_rec
def createFEATUREannot(loc_range, featuretype, s):
""" Creates a new SeqFeature with ExactPositions based on range."""
location = SeqFeature.FeatureLocation(
SeqFeature.ExactPosition(loc_range[0]),
SeqFeature.ExactPosition(loc_range[1]))
new_feature = SeqFeature.SeqFeature(location, type=featuretype, strand=s)
return (new_feature)
def ins_tag(self,tag_seq,protease_seq,ins_name,ins_sites,side=5): #the cutpoint is after ins_sites[0] bp and after ins_sites[1] bp
from Bio.Alphabet import IUPAC
from Bio.Seq import Seq
from Bio.SeqRecord import SeqRecord
for feature in self.record.features:
if feature.qualifiers.has_key("note")\
and re.search(r"^mcs",feature.qualifiers["note"][0],re.I):
mcs_start=int(str(feature.location.start))
mcs_end=int(str(feature.location.end))
mcs_qualifiers=feature.qualifiers
if ins_sites[0]>mcs_start and ins_sites[1]<mcs_end:
f_mcs=SeqFeature(FeatureLocation(mcs_start,
mcs_end+ins_sites[0]-ins_sites[1]+len(tag_seq+protease_seq)),type="mcs")
f_mcs.qualifiers=mcs_qualifiers
if side==5:
ins_record=SeqRecord(Seq(tag_seq+protease_seq,IUPAC.ambiguous_dna))
f=SeqFeature(FeatureLocation(0,len(tag_seq)),type="tag")
elif side==3:
ins_record=SeqRecord(Seq(protease_seq+tag_seq,IUPAC.ambiguous_dna))
f=SeqFeature(FeatureLocation(len(protease_seq),len(protease_seq+tag_seq)),type="tag")
f.qualifiers["note"]=[ins_name,]
ins_record.features=[f]
old_name=self.record.name
self.record=self.record[:ins_sites[0]]+ins_record+self.record[ins_sites[1]:]
self.record.name=old_name
self.whole_len=len(self.record)
self.record.features.append(f_mcs)
self.record.features=sorted(self.record.features,key=lambda x:int(str(x.location.start)))
def _make_position(location_string, offset=0):
"""Turn a Swiss location position into a SeqFeature position object (PRIVATE).
An offset of -1 is used with a start location to make it pythonic.
"""
if location_string == "?":
return SeqFeature.UnknownPosition()
# Hack so that feature from 0 to 0 becomes 0 to 0, not -1 to 0.
try:
return SeqFeature.ExactPosition(max(0, offset + int(location_string)))
except ValueError:
pass
if location_string.startswith("<"):
try:
return SeqFeature.BeforePosition(
max(0, offset + int(location_string[1:])))
except ValueError:
pass
elif location_string.startswith(">"): # e.g. ">13"
try:
return SeqFeature.AfterPosition(
max(0, offset + int(location_string[1:])))
except ValueError:
pass
elif location_string.startswith("?"): # e.g. "?22"
try:
return SeqFeature.UncertainPosition(
max(0, offset + int(location_string[1:])))
except ValueError:
pass
raise NotImplementedError("Cannot parse location '%s'" % location_string)
def al_string2feat(queryseq, ampsdict): #lib5pr is subjectseq; t7 is queryseq
'''
This function accepts a query seq and a dictionary of subjectseqs, where the key (amp)
is contained in a field in queryseq, highlighting the location of queryseq in it.
Returns a string.
'''
subjectseq = SeqRecord(ampsdict[queryseq[1][0]])
#for seqrecord in subjectseq:
locstart = queryseq[1][1]
#print queryseq
locend = queryseq[1][2]
fwdlocs = []
revlocs = []
# Figure out which strand the BLAST hit is on
if locstart <= locend:
fwdlocs.append(locstart)
if locstart > locend:
revlocs.append(locend)
for item in fwdlocs:
start = ExactPosition(int(item))
end = ExactPosition(int((item) + len(queryseq[0].seq) + 1))
location = FeatureLocation(start, end)
feature = SeqFeature(location,type=str("cutsite_fwd"), strand = +1)
subjectseq.features.append(feature)
for item in revlocs:
start = ExactPosition(int(item))
end = ExactPosition(start + len(queryseq[0].seq))
location = FeatureLocation(start, end)
feature = SeqFeature(location,type=str("cutsite_rev"), strand = -1)
subjectseq.features.append(feature)
#print subjectseq.features
return subjectseq
def _get_feature(self, feature_dict):
"""Retrieve a Biopython feature from our dictionary representation.
"""
location = SeqFeature.FeatureLocation(*feature_dict['location'])
new_feature = SeqFeature.SeqFeature(location, feature_dict['type'],
id=feature_dict['id'], strand=feature_dict['strand'])
new_feature.qualifiers = feature_dict['quals']
return new_feature
def _make_seqfeature(name, from_res, to_res, description, ft_id):
"""Construct SeqFeature from feature data from parser (PRIVATE)."""
loc = SeqFeature.FeatureLocation(_make_position(from_res, -1),
_make_position(to_res, 0))
if not ft_id:
ft_id = "<unknown id>" # The default in SeqFeature object
return SeqFeature.SeqFeature(loc, type=name, id=ft_id,
qualifiers={"description": description})
def create_feature(sequence, name, start, end, strand=+1):
if str(name) and int(start) and int(end):
my_feature_location = SeqFeature.FeatureLocation(start,
end,
strand=strand)
my_feature = SeqFeature.SeqFeature(my_feature_location, type=name)
sequence.features.append(my_feature)
def add_features(block, allblocks, gb, start):
# Disregard fillers... don't create features for them
if is_filler(block):
return start + block["sequence"]["length"]
# For handling list blocks!
if "current_option" in block:
option = [b for b in allblocks if b["id"] == block["current_option"]][0]
return add_features(option, allblocks, gb, start)
# Add Myself as a feature
sf = SeqFeature.SeqFeature()
# Set the type based on the original type or the role type
if "genbank" in block["metadata"] and "type" in block["metadata"]["genbank"]:
sf.type = block["metadata"]["genbank"]["type"]
elif "rules" in block and "role" in block["rules"] and block["rules"]["role"] is not None and block["rules"]["role"] != "":
sf.type = block["rules"]["role"]
else:
sf.type = "misc_feature"
# Set up the location of the feature
feature_strand = 1
if "strand" in block["metadata"]:
feature_strand = block["metadata"]["strand"]
# And copy all the other qualifiers that came originally from genbank
if "genbank" in block["metadata"]:
for annot_key, annot_value in block["metadata"]["genbank"].iteritems():
if annot_key not in ["name_source", "note"]:
sf.qualifiers[annot_key] = annot_value
convert_block_name(sf, block)
add_GC_info(sf, block, allblocks)
convert_annotations(block, gb, start)
# Add my children as features
child_start = start
for i in range(0, len(block["components"])):
block_id = block["components"][i]
bl = [b for b in allblocks if b["id"] == block_id][0]
child_start = add_features(bl, allblocks, gb, child_start)
if child_start != start:
# The end is where the last child ended...
end = child_start
else:
# No children, look at the block's length
if "sequence" in block:
end = start + block["sequence"]["length"]
else:
end = start
sf.location = SeqFeature.FeatureLocation(start, end, strand=feature_strand)
gb.features.append(sf)
return end
def spacersonly(seqs):
sgRNAconst = SeqRecord(Seq("GTTTAAGAG"))
while True:
seqrecord = seqs.next()
#for seqrecord in seqs:
fwdlocs = []
revlocs = []
fwdlocs = [
tloc.start()
for tloc in re.finditer(str(sgRNAconst.seq), str(seqrecord.seq))
]
for item in fwdlocs:
start = ExactPosition(int(item) + 1)
end = ExactPosition(int((item) + len(sgRNAconst)))
location = FeatureLocation(start, end)
feature = SeqFeature(location, type="sgRNAconst", strand=+1)
seqrecord.features.append(feature)
revlocs = [
tloc.start() for tloc in re.finditer(
str(sgRNAconst.reverse_complement().seq), str(seqrecord.seq))
]
for item in revlocs:
start = ExactPosition(int(item) + 1)
end = ExactPosition(start + len(sgRNAconst) - 1)
location = FeatureLocation(start, end)
feature = SeqFeature(location, type="sgRNAconst", strand=-1)
seqrecord.features.append(feature)
for feat in seqrecord.features:
if feat.strand == 1:
tgtstart = int(feat.location.start) - 36 # -21
tgtend = int(feat.location.start) - 1
sgtgt = seqrecord[tgtstart:tgtend]
#yield sgtgt
#alltgts.append(sgtgt)
#print "pos \n \n"
if feat.strand == -1:
tgtend = int(feat.location.end) + 36 # +21
tgtstart = int(feat.location.end)
sgtgt = seqrecord[tgtstart:tgtend].reverse_complement()
sgtgt.name = seqrecord.name
#yield sgtgt
#alltgts.append(sgtgt)
bad = 0
try:
l = [
tloc.end()
for tloc in re.finditer("ACTCACTATAG", str(sgtgt.seq))
]
sgtgt = sgtgt[int(l[0]):]
except:
None
for score in sgtgt.letter_annotations["phred_quality"]:
if score < 30:
bad = 1
if bad == 0 and len(sgtgt) > 10:
yield sgtgt
break
def translateFeatureLocation(location, region, translation=0):
location2 = location + translation + 1
if location2.end < 0:
logging.debug('Error-prone feature detected: {}'.format(location2))
return SeqFeature.FeatureLocation(start=0, end=0, strand=0)
else:
return SeqFeature.FeatureLocation(start=max(0, location2.start),
end=min(location2.end, region.end),
strand=location2.strand)
def addFeatureComplSTF():
if m.end() <= seqLength:
newFeature = SeqFeature(FeatureLocation(m.start(),m.end(), strand=-1), type=str(feature))
newFeature.qualifiers['note'] = featureName
newRecord.features.append(newFeature)
else:
newFeature = SeqFeature(CompoundLocation([FeatureLocation(m.start(),seqLength, strand=-1), FeatureLocation(1, (seqLength - m.end()), strand=-1)]), type=str(feature))
newFeature.qualifiers['note'] = featureName
newRecord.features.append(newFeature)
def test_GenerateFeatLoc__make_start_fuzzy__1(self):
''' Test to evaluate function `make_start_fuzzy` of class `GenerateFeatLoc`.
This test evaluates the case where FeatureLocations are made fuzzy. '''
from Bio import SeqFeature
start_pos = SeqFeature.ExactPosition(5)
end_pos = SeqFeature.ExactPosition(9)
location_object = SeqFeature.FeatureLocation(start_pos, end_pos)
out = GnOps.GenerateFeatLoc().make_start_fuzzy(location_object)
self.assertIsInstance(out, Bio.SeqFeature.FeatureLocation) # FeatureLocation
self.assertIsInstance(out.start, Bio.SeqFeature.BeforePosition) # Fuzzy Start
def contig_info(contig_id, contig_seq, species_informations):
"""
Create contig information from species_informations dictionary and contig id and contig seq.
"""
record = SeqRecord(contig_seq,
id=contig_id,
name=contig_id,
description=species_informations['description'],
annotations={"molecule_type": "DNA"})
if IUPAC:
record.seq.alphabet = IUPAC.ambiguous_dna
if 'data_file_division' in species_informations:
record.annotations['data_file_division'] = species_informations[
'data_file_division']
record.annotations['date'] = datetime.date.today().strftime(
'%d-%b-%Y').upper()
if 'topology' in species_informations:
record.annotations['topology'] = species_informations['topology']
record.annotations['accessions'] = contig_id
if 'organism' in species_informations:
record.annotations['organism'] = species_informations['organism']
# Use of literal_eval for taxonomy and keywords to retrieve list.
if 'taxonomy' in species_informations:
record.annotations['taxonomy'] = species_informations['taxonomy']
if 'keywords' in species_informations:
record.annotations['keywords'] = species_informations['keywords']
if 'source' in species_informations:
record.annotations['source'] = species_informations['source']
new_feature_source = sf.SeqFeature(sf.FeatureLocation(
1 - 1, len(contig_seq)),
type="source")
new_feature_source.qualifiers['scaffold'] = contig_id
if 'isolate' in species_informations:
new_feature_source.qualifiers['isolate'] = species_informations[
'isolate']
# db_xref corresponds to the taxon NCBI ID.
# Important if you want to use Pathway Tools after.
if 'db_xref' in species_informations:
new_feature_source.qualifiers['db_xref'] = species_informations[
'db_xref']
if 'cell_type' in species_informations:
new_feature_source.qualifiers['cell_type'] = species_informations[
'cell_type']
if 'dev_stage' in species_informations:
new_feature_source.qualifiers['dev_stage'] = species_informations[
'dev_stage']
if 'mol_type' in species_informations:
new_feature_source.qualifiers['mol_type'] = species_informations[
'mol_type']
record.features.append(new_feature_source)
return record
def _parse_dbReference(element):
self.ParsedSeqRecord.dbxrefs.append(element.attrib["type"] + ":" +
element.attrib["id"])
if "type" in element.attrib:
# <dbReference type="EMBL" id="U96180">
# <property type="protein sequence ID" value="AAB66902.1"/>
# <property type="molecule type" value="mRNA"/>
# </dbReference>
if element.attrib["type"] == "EMBL":
for ref_element in element:
if "type" in ref_element.attrib and "value" in ref_element.attrib:
if ref_element.attrib[
"type"] == "protein sequence ID":
self.ParsedSeqRecord.dbxrefs.append(
"EMBL-CDS:" + ref_element.attrib["value"])
# e.g.
# <dbReference type="PDB" key="11" id="2GEZ">
# <property value="X-ray" type="method"/>
# <property value="2.60 A" type="resolution"/>
# <property value="A/C/E/G=1-192, B/D/F/H=193-325" type="chains"/>
# </dbReference>
elif element.attrib["type"] == "PDB":
method = ""
resolution = ""
for ref_element in element:
if ref_element.tag == NS + "property":
dat_type = ref_element.attrib["type"]
if dat_type == "method":
method = ref_element.attrib["value"]
if dat_type == "resolution":
resolution = ref_element.attrib["value"]
if dat_type == "chains":
pairs = ref_element.attrib["value"].split(",")
for elem in pairs:
pair = elem.strip().split("=")
if pair[1] != "-":
# TODO - How best to store these, do SeqFeatures make sense?
feature = SeqFeature.SeqFeature()
feature.type = element.attrib["type"]
feature.qualifiers[
"name"] = element.attrib["id"]
feature.qualifiers["method"] = method
feature.qualifiers[
"resolution"] = resolution
feature.qualifiers["chains"] = pair[
0].split("/")
start = int(pair[1].split("-")[0]) - 1
end = int(pair[1].split("-")[1])
feature.location = SeqFeature.FeatureLocation(
start, end)
# self.ParsedSeqRecord.features.append(feature)
for ref_element in element:
if ref_element.tag == NS + "property":
pass # this data cannot be fitted in a seqrecord object with a simple list. however at least ensembl and EMBL parsing can be improved to add entries in dbxrefs
def _get_feature(self, feature_dict):
"""Retrieve a Biopython feature from our dictionary representation.
"""
location = SeqFeature.FeatureLocation(*feature_dict['location'])
new_feature = SeqFeature.SeqFeature(location, feature_dict['type'],
id=feature_dict['id'], strand=feature_dict['strand'])
# Support for Biopython 1.68 and above, which removed sub_features
if not hasattr(new_feature, "sub_features"):
new_feature.sub_features = []
new_feature.qualifiers = feature_dict['quals']
return new_feature
def _trans_loc(loc):
# Don't write the contig ID in the loc line unless it's trans-spliced
if loc[0] == current_contig_id:
loc[0] = None
if loc[2] == "-":
return SeqFeature.FeatureLocation(loc[1] - loc[3], loc[1], -1,
loc[0])
else:
return SeqFeature.FeatureLocation(loc[1] - 1,
loc[1] + loc[3] - 1, 1,
loc[0])
def create_genbank(gene_nucleic_seqs, gene_protein_seqs, annot, go_namespaces,
go_alternatives, output_path, species_informations):
""" Create genbank file from nucleic and protein fasta plus eggnog mapper annotation file.
Args:
gene_nucleic_seqs (dict): dictionary of nucleic sequences (key: sequence id, value: sequence)
gene_protein_seqs (dict): dictionary of protein sequences (key: sequence id, value: sequence)
annot (dict): dictionary of eggnog-ammper annotation (key: gene_id, value: ['GOs','EC', 'Preferred_name'])
go_namespaces (dict): dictionary of GO terms namespace (key: GO Term ID, value: namespace associated to GO Term)
go_alternatives (dict): dictionary of GO terms alternatives ID (key: GO Term ID, value: alternatives GO Term associated to GO Term)
output_path (str): output file or directory
species_informations (dict): dictionary containing information about species
"""
# All SeqRecord objects will be stored in a list and then give to the SeqIO writer to create the genbank.
records = []
# Iterate through each contig/gene.
for gene_nucleic_id in sorted(gene_nucleic_seqs):
# Create a SeqRecord object using gene information.
record = record_info(gene_nucleic_id,
gene_nucleic_seqs[gene_nucleic_id],
species_informations)
# If id is numeric, change it
if gene_nucleic_id.isnumeric():
id_gene = f"gene_{gene_nucleic_id}"
elif "|" in gene_nucleic_id:
id_gene = gene_nucleic_id.split("|")[1]
else:
id_gene = gene_nucleic_id
start_position = 1
end_position = len(gene_nucleic_seqs[gene_nucleic_id])
strand = 0
new_feature_gene = sf.SeqFeature(sf.FeatureLocation(
start_position, end_position, strand),
type="gene")
new_feature_gene.qualifiers['locus_tag'] = id_gene
# Add gene information to contig record.
record.features.append(new_feature_gene)
new_cds_feature = create_cds_feature(id_gene, start_position,
end_position, strand, annot,
go_namespaces, go_alternatives,
gene_protein_seqs)
new_cds_feature.qualifiers['locus_tag'] = id_gene
# Add CDS information to contig record
record.features.append(new_cds_feature)
records.append(record)
# Create Genbank with the list of SeqRecord.
SeqIO.write(records, output_path, 'genbank')
def test_GenerateFeatLoc__make_start_fuzzy__3(self):
''' Test to evaluate function `make_start_fuzzy` of class `GenerateFeatLoc`.
This test evaluates if end FeatureLocations are made fuzzy.
See AfterPosition. '''
from Bio import SeqFeature
start_pos = SeqFeature.ExactPosition(5)
end_pos = SeqFeature.ExactPosition(9)
location_object = SeqFeature.FeatureLocation(start_pos, end_pos)
out = GnOps.GenerateFeatLoc().make_end_fuzzy(location_object)
self.assertIsInstance(out, Bio.SeqFeature.FeatureLocation) # FeatureLocation
self.assertIsInstance(out.end, Bio.SeqFeature.AfterPosition) # Fuzzy End
def _get_feature(self, feature_dict):
"""Retrieve a Biopython feature from our dictionary representation."""
location = SeqFeature.FeatureLocation(*feature_dict["location"])
new_feature = SeqFeature.SeqFeature(
location,
feature_dict["type"],
id=feature_dict["id"],
strand=feature_dict["strand"],
)
new_feature.qualifiers = feature_dict["quals"]
return new_feature
def convert_annotations(block, gb, start):
if "sequence" not in block:
return
# Add My annotations as features
for annotation in block["sequence"]["annotations"]:
gb_annot = SeqFeature.SeqFeature()
annotation_type = "misc_feature"
if "role" in annotation and annotation["role"] != "":
annotation_type = annotation["role"]
for key, value in annotation.iteritems():
if key not in [
"start", "end", "notes", "strand", "color", "role",
"isForward"
]:
gb_annot.qualifiers[key] = value
elif key == "notes" and "genbank" in annotation["notes"]:
for gb_key, gb_value in annotation["notes"][
"genbank"].iteritems():
if gb_key not in ["type", "note"]:
gb_annot.qualifiers[gb_key] = gb_value
elif gb_key == "type":
annotation_type = gb_value
gc_info = {
"GC": {
"name": annotation["name"],
"type": "annotation",
"parents": [block["id"]]
}
}
if "color" in annotation:
gc_info["GC"]["color"] = annotation["color"]
if "notes" in annotation and "genbank" in annotation[
"notes"] and "note" in annotation["notes"]["genbank"]:
gc_info["note"] = annotation["notes"]["genbank"]["note"]
gb_annot.qualifiers["note"] = json.dumps(gc_info).replace("\"", "'")
if "start" in annotation:
strand = 1
if "isForward" in annotation and annotation["isForward"] == -1:
strand = -1
# Remember: annotations start and end are relative to the block
gb_annot.location = SeqFeature.FeatureLocation(
annotation["start"] + start, annotation["end"] + start + 1,
strand)
gb_annot.type = annotation_type
gb.features.append(gb_annot)
def add_feature(sequence_rec, start_postion, end_position, strand, name,
feature_type, feature_id):
# add a feature to the seq record
my_feature_location = SeqFeature.FeatureLocation(start_postion - 1,
end_position,
strand=strand)
my_feature = SeqFeature.SeqFeature(my_feature_location,
type=feature_type,
id=feature_id)
my_feature.qualifiers["label"] = name
return my_feature
def to_seq_feature(self):
quals = {}
for q in self.qualifiers.all():
quals[q.name] = q.data
s = None
if self.direction == 'f':
s = 1
elif self.direction == 'r':
s = -1
return SeqFeature.SeqFeature(location=SeqFeature.FeatureLocation(
self.start, self.end),
type=self.type,
strand=s,
qualifiers=quals)
def make_feature(product, blast_qresult, fragment ,hit, hsp, fragstart, count):
s = hsp.hit_start
e = hsp.hit_end
if product == 'YR':
s = hsp.hit_start-int(fragstart)
e = hsp.hit_end-int(fragstart)
feature = SeqFeature(FeatureLocation(s, e), type="DOMAIN", strand= hsp.hit_strand)
feature.qualifiers['loc_on_contig'] = str(hsp.hit_start+1) + '..' + str(hsp.hit_end)
feature.qualifiers['product'] = product
feature.qualifiers['serial_on_frag'] = count
count += 1
feature.qualifiers['program'] = blast_qresult.program + "_" + blast_qresult.version
feature.qualifiers['evalue'] = hsp.evalue
feature.qualifiers['assembly'] = blast_qresult.target.split('/')[-1]
feature.qualifiers['contig'] = contig
feature.qualifiers['translation'] = feature.extract(fragment.seq).translate()
return (feature, count)
def writePBS():
global variation, seqRecordToCheck, seqRecordToCheckComplement, difference, newFeature
for variation in featureStatistic_container[feature]:
primerSeq = str(variation.seq)
primerName = variation.note
partialPrimerSeq = primerSeq[len(primerSeq) - 15::]
seqRecordToCheck = str(record.seq)
seqRecordToCheckComplement = str(reverse_complement(record.seq))
matchingPrimerPositions = SeqUtils.nt_search(seqRecordToCheck, partialPrimerSeq)
if (len(matchingPrimerPositions) > 1):
difference = len(primerSeq) - len(partialPrimerSeq)
length = len(matchingPrimerPositions)
for j in range(1, length):
if primerSeq == seqRecordToCheck[matchingPrimerPositions[j] -
difference: matchingPrimerPositions[j] - difference + len(primerSeq)]:
newFeature = SeqFeature(FeatureLocation(matchingPrimerPositions[j],
matchingPrimerPositions[j] + len(primerSeq),
strand=1), type=str(feature))
newFeature.qualifiers['note'] = primerName
newRecord.features.append(newFeature)
else:
newFeature = SeqFeature(FeatureLocation(matchingPrimerPositions[j], AfterPosition(
matchingPrimerPositions[j] + len(primerSeq)), strand=1), type=str(feature))
newFeature.qualifiers['note'] = primerName
newRecord.features.append(newFeature)
matchingPrimerPositions = SeqUtils.nt_search(seqRecordToCheckComplement, partialPrimerSeq)
if (len(matchingPrimerPositions) > 1):
difference = len(primerSeq) - len(partialPrimerSeq)
length = len(matchingPrimerPositions)
for j in range(1, length):
if primerSeq == seqRecordToCheckComplement[matchingPrimerPositions[j] -
difference: matchingPrimerPositions[j] - difference + len(primerSeq)]:
newFeature = SeqFeature(FeatureLocation(matchingPrimerPositions[j],
matchingPrimerPositions[j] + len(primerSeq),
strand=-1), type=str(feature))
newFeature.qualifiers['note'] = primerName
newRecord.features.append(newFeature)
else:
newFeature = SeqFeature(FeatureLocation(matchingPrimerPositions[j], AfterPosition(
matchingPrimerPositions[j] + len(primerSeq)), strand=-1), type=str(feature))
newFeature.qualifiers['note'] = primerName
newRecord.features.append(newFeature)
def ins_insert(self,vec_5_site,utr_5_seq,ins_seq,utr_3_seq,vec_3_site,ins_name):
from Bio.Alphabet import IUPAC
from Bio.Seq import Seq
from Bio.SeqRecord import SeqRecord
ins_record=SeqRecord(Seq(utr_5_seq+ins_seq+utr_3_seq,IUPAC.ambiguous_dna))
f_i=SeqFeature(FeatureLocation(len(utr_5_seq),len(utr_5_seq)+len(ins_seq)),type="insert")
f_i.qualifiers["note"]=[ins_name,]
ins_record.features=[f_i]
old_name=self.record.name
for feature in self.record.features:
if feature.qualifiers.has_key("note")\
and re.search(r"^mcs",feature.qualifiers["note"][0],re.I):
mcs_start=int(str(feature.location.start))
mcs_end=int(str(feature.location.end))
mcs_qualifiers=feature.qualifiers
self.record=self.record[:vec_5_site]+ins_record+self.record[vec_3_site:]
f_mcs=SeqFeature(FeatureLocation(mcs_start,
vec_5_site+len(utr_5_seq+ins_seq+utr_3_seq)+mcs_end-vec_3_site),type="mcs")
f_mcs.qualifiers=mcs_qualifiers
self.record.features.append(f_mcs)
self.record.features=sorted(self.record.features,key=lambda x:int(str(x.location.start)))
self.record.name=old_name
self.whole_len=len(self.record)
def writeFeature(strand):
global newFeature
if (len(occurrence) > 1):
for i in range(1, len(occurrence)):
newFeature = SeqFeature(FeatureLocation(occurrence[i], occurrence[i] + len(featureSeq), strand=strand),
type=str(feature))
if variation.product is not None:
newFeature.qualifiers['product'] = variation.product
if variation.gene is not None:
newFeature.qualifiers['gene'] = variation.gene
if variation.bound_moiety is not None:
newFeature.qualifiers['bound_moiety'] = variation.bound_moiety
if variation.mobile is not None:
newFeature.qualifiers['mobile'] = variation.mobile
if variation.note is not None:
newFeature.qualifiers['note'] = variation.note
newRecord.features.append(newFeature)
# .WithinPosition: position between two nucleotides '(1.5)' in this way: position 1 is lower boundary, extension 4 is range to higher boundary
# .OneOfPosition: any of a list of several numbers
# .UnknownPosition: position of unknown location.
from Bio import SeqFeature
start_pos = SeqFeature.AfterPosition(5)
end_pos = SeqFeature.BetweenPosition(9, left=8, right=9)
mylocation = SeqFeature.FeatureLocation(start_pos, end_pos)
print mylocation, mylocation.start, mylocation.end, int(mylocation.end)
for feature in record.features:
if 4350 in feature: # if position 4350 is in any feature
print feature.type, feature.qualifiers.get('db_xref')
from Bio.SeqFeature import SeqFeature, FeatureLocation
seqParent = Seq('ACCGAGACGGCAAAGGCTAGCATAGGTATGAGACTTCCTTCCTGCCAGTGCTGAGGAACTGGGAGCCTAC')
featu = SeqFeature(FeatureLocation(5, 18), type='gene', strand=-1) # location [5:18] in reverse_complement
print featu
featureSeq = seqParent[featu.location.start:featu.location.end].reverse_complement()
print featureSeq
print featu.extract(seqParent), len(featu.extract(seqParent)), len(featu), len(featu.location)
# extract gets the subseq in location featu from seqParent
# References publications that mention it
# Bio.SeqFeature.Reference
# journal: book, magazine, journal name
# title, authors: of the paper
# medline_id, pubmed_id: ID en Medline y PubMed
# comment: about the reference
# location: to specify location in the sequence mentioned in the paper
# format: method to output as fasta or genbank formatted seq
if filename == inverted_repeats_file:
direction = 'inverted'
lines = open(filename, 'r').readlines()
readl = 0
for line in lines:
if line[0:9] == 'FASTA_HDR': #Get fragment name
parts = re.split(r'\s+',line)
fragment = ':'.join(parts[1].split(':')[0:-1])
if not fragment in repeat_features.keys():
repeat_features[fragment] = []
elif line[0:8] == 'FEATURES': #Start to read feature lines
readl = 1
elif line[0:6] == 'ORIGIN': #Stop reading feature lines and ...
readl = 0
if not end_a == 0: #If there were features, put the last one in repeat_features
feature = SeqFeature(FeatureLocation(int(start_a)-1, int(end_a)), type="REPEAT", id = direction + '_' + str(repeat_name) + '.1') #Make a SeqFeature object for the first repeat partenr
for mod in modifyers.keys():
feature.qualifiers[mod] = modifyers[mod] #add the qualifiers to the SeqFeature object
feature.qualifiers['name'] = direction + '_' + str(repeat_name) + '.1'
repeat_features[fragment].append(feature)
feature = SeqFeature(FeatureLocation(int(start_b)-1, int(end_b)), type="REPEAT", id = direction + '_' + str(repeat_name) + '.2') #Make a SeqFeature object for the second repeat partenr
for mod in modifyers.keys():
feature.qualifiers[mod] = modifyers[mod] #add the qualifiers to the SeqFeature object
feature.qualifiers['name'] = direction + '_' + str(repeat_name) + '.2'
#print ' Got repeat ' + feature.qualifiers['name'] + ' on fragment ' + fragment
repeat_features[fragment].append(feature)
repeat_name = 1 #Roll back all parameters to null for the next fragment
start_a = 0
end_a = 0
start_b = 0
end_b = 0