def parse(inFq, inDomtblout, inProtFna=None):
"""
Read in joined pair-end reads and its HMM annotation from the FASTQ, DOMTBLOUT, (and optionally PROT FASTA file)
@param inFq: FASTQ file containing joined pair-end reads
@param inDomtblout: HMM annotation file
@param inProtFna: corresponding prot sequence (can be None)
@type inFq: str
@type inDomtblout: str
@type inProtFna: str | None
@rtype: list[read_rec.ReadRec]
@return: a list of read-records
"""
recList = []
# read in prot sequences
if inProtFna is None:
nameToProtSeq = {}
else:
nameToProtSeq = fas.fastaFileToDictWholeNames(inProtFna)
# read in dom file
nameToDom = hmm.readDomblout(inDomtblout)
assert inProtFna is None or len(nameToProtSeq) == len(nameToDom)
# read in pair-end reads, create ReadRec
for readName, dna, comment, qs in fq.ReadFqGen(inFq):
readName = readName[1:] # strip starting @
protSeq = nameToProtSeq.get(readName, None)
hit, frameTag = nameToDom[readName]
annotStart, annotLen, strain, score, acc = hmm.dnaHitInfo(hit, dna, protSeq) # strain, score, acc not used
if strain == 1:
assert 1 <= frameTag <= 3
else:
assert 4 <= frameTag <= 6
# alignment env coord
protStart = int(hit[19]) - 1
protLen = int(hit[20]) - protStart
assert annotLen == 3 * protLen
# alignment coord
protStartAli = int(hit[17]) - 1
protLenAli = int(hit[18]) - protStartAli
# hmm coordinates
hmmCoordStart = int(hit[15]) - 1
hmmCoordLen = int(hit[16]) - hmmCoordStart
# the env coordinates start (and end) often before the alignment coordinates and end after, get the offsets
offsetEnv = protStartAli - protStart
offsetEnvE = protLen - offsetEnv - protLenAli
assert offsetEnv >= 0
assert offsetEnvE >= 0
hmmCoordStart -= offsetEnv
hmmCoordLen += offsetEnv + offsetEnvE
tokens = comment.split('\t')
if len(tokens) == 5:
# get the ends of the pair-end read and corresponding quality-scores
p, dna1, qs1, dna2, qs2 = tokens
# get the QSArray representation of the first read-end
qsA1 = qs_man.QSArray(dna=dna1, qsArrayFq=qs1)
# get the reverse complement of the second read-end
qsA2 = qs_man.QSArray(dna=dna2, qsArrayFq=qs2)
qsA2.revCompl()
# get the consensus QS Array representing of the joined read
qsA = qs_man.QSArray(qsA1=qsA1, qsA2=qsA2, pos1Within2=len(dna2) - len(dna))
else:
# there is just a simple dna sequence (i.e. not joined reads)
qsA = qs_man.QSArray(dna=dna, qsArrayFq=qs)
recList.append(read_rec.ReadRec(readName, qsA, frameTag, annotStart, annotLen, hmmCoordStart, hmmCoordLen,
protSeq, protStart, protLen))
return recList
def partitionReads(sampleDir, scoreThreshold, accuracyThreshold, shuffleRandSeed, pfamPartitionedDir, joinedReads=True,
considerSam=True):
"""
Partitioning reads into the individual gene-domains.
"""
try:
strainDirList = map(lambda x: os.path.join(sampleDir, x), os.listdir(sampleDir))
samplePartDir = os.path.join(sampleDir, pfamPartitionedDir)
if not os.path.isdir(samplePartDir):
os.mkdir(samplePartDir)
# for each gene-dom
fqOutDict = {}
fqProtOutDict = {}
fqSamOutDict = {}
fqDomOutDict = {}
for strainDir in strainDirList:
strainAcc = os.path.basename(strainDir)
if strainAcc == 'sample_partitioned': # skip the directory containing the partitioned data
continue
if os.path.isdir(strainDir):
# for each dom file
for f in os.listdir(strainDir):
if (joinedReads and f.endswith('join_prot.domtblout.gz')) \
or (not joinedReads and f.endswith('pair1_prot.domtblout.gz')): # a domtblout file found
i = f.split('_', 1)[0]
if i.isdigit():
if joinedReads:
domPath = os.path.join(strainDir, f)
domPath2 = None
fqPath = os.path.join(strainDir, '%s_join.fq.gz' % (i,))
fqPair1Path = os.path.join(strainDir, '%s_pair1.fq.gz' % (i,))
fqPair2Path = os.path.join(strainDir, '%s_pair2.fq.gz' % (i,))
fqProtPath = os.path.join(strainDir, '%s_join_prot.fna.gz' % (i,))
fqProtPath2 = None
samPath = os.path.join(strainDir, '%s_join_gmap.sam.gz' % (i,))
assert os.path.isfile(fqPath)
else:
domPath = os.path.join(strainDir, f)
domPath2 = os.path.join(strainDir, '%s_pair2_prot.domtblout.gz' % (i,))
fqPath = None
fqPair1Path = os.path.join(strainDir, '%s_pair1.fq.gz' % (i,))
fqPair2Path = os.path.join(strainDir, '%s_pair2.fq.gz' % (i,))
fqProtPath = os.path.join(strainDir, '%s_pair1_prot.fna.gz' % (i,))
fqProtPath2 = os.path.join(strainDir, '%s_pair2_prot.fna.gz' % (i,))
samPath = os.path.join(strainDir, '%s_pair.sam.gz' % (i,))
assert os.path.isfile(domPath2) and os.path.isfile(fqProtPath2)
assert os.path.isfile(domPath) and os.path.isfile(fqPair1Path) \
and os.path.isfile(fqPair2Path) and os.path.isfile(fqProtPath)
if considerSam:
assert os.path.isfile(samPath)
# map: read-name -> list of hits (lists sorted according to the scores)
nameToHitList = getReadNameToHitList(domPath)
if not joinedReads:
nameToHitList2 = getReadNameToHitList(domPath2)
len1 = len(nameToHitList)
len2 = len(nameToHitList2)
nameToHitList.update(nameToHitList2)
assert len(nameToHitList) == len1 + len2
# map: read-name-prot -> seq-prot
protNameToSeq = fas.fastaFileToDictWholeNames(fqProtPath)
if not joinedReads:
protNameToSeq.update(fas.fastaFileToDictWholeNames(fqProtPath2))
# map: read-name -> sam-line-entry
if considerSam:
readNameToSam = {}
if joinedReads:
for line in gzip.open(samPath):
line = line.strip()
if line.startswith('#'):
continue
readName = line.split('\t', 1)[0]
# lines with only 11 entries will be padded with * to 12
if len(line.split('\t')) == 11:
line += '\t*'
readNameToSam[readName] = line + '\t' + strainAcc
else:
entry = []
for line in gzip.open(samPath):
line = line.strip()
if line.startswith('#') or line.startswith('@'):
continue
if len(entry) < 2:
entry.append(line)
if len(entry) == 2:
readName = entry[0].split('\t', 1)[0]
assert readName == entry[1].split('\t', 1)[0]
readNameToSam[readName] = entry[0] + '\t*\t' + strainAcc + '\n' \
+ entry[1] + '\t*\t' + strainAcc
entry = []
# map read-name -> "pair1-dna tab pair1-qs tab pair2-dna tab pair2-qs"
if joinedReads:
readNameToPairReads = fq.getFqToDict(fqPair1Path, fqPair2Path)
else:
readNameToPairReads = None
if joinedReads:
g1 = fq.ReadFqGen(fqPath)
g2 = []
else:
g1 = fq.ReadFqGen(fqPair1Path)
g2 = fq.ReadFqGen(fqPair2Path)
# go over all reads
for readName, dna, p, qs in list(g1) + list(g2):
readName = readName[1:] # strip starting '@'
# take the hit with the highest score
topHit = None
if readName in nameToHitList:
topHit = nameToHitList[readName][0]
# is the hit significant, filter according to the score and accuracy
if topHit is None or float(topHit[13]) < scoreThreshold or float(topHit[21]) < accuracyThreshold:
continue
else:
famName = topHit[3]
if famName not in fqOutDict:
fqOutDict[famName] = []
fqProtOutDict[famName] = []
fqSamOutDict[famName] = []
fqDomOutDict[famName] = []
if joinedReads:
comment = readNameToPairReads[readName]
else:
comment = ''
fqOutDict[famName].append((readName, dna, qs, comment))
protSeqName = topHit[0]
protSeq = protNameToSeq[protSeqName]
fqProtOutDict[famName].append((readName, protSeq))
if considerSam:
if joinedReads:
fqSamOutDict[famName].append(readNameToSam[readName])
else:
fqSamOutDict[famName].append(readNameToSam[readName[:-2]])
# top hit coordinates within the read
startOnRead, overlapLen, strain = hmm.dnaHitInfo(topHit, dna, protSeq)[:3]
fqDomOutDict[famName].append('\t'.join(topHit) + '\t%s\t%s\t%s' % (startOnRead, overlapLen, strain))
if joinedReads:
ident = 'join'
else:
ident = 'pair'
# for each gene dom, store reads into a file
for famName, fqContentList in fqOutDict.iteritems():
# get the tagged fam-dom-name that can be used in file names
pf = comh.getGeneNameToFileName(famName)[:-4]
# define output files
fqOutO = os.path.join(samplePartDir, 'o_%s_%s.fq.gz' % (pf, ident)) # 'o_' ~ ordered entries
fqOutR = os.path.join(samplePartDir, 'r_%s_%s.fq.gz' % (pf, ident)) # 'r_' ~ random shuffled entries
fqProtOutO = os.path.join(samplePartDir, 'o_%s_%s_prot.fna.gz' % (pf, ident))
fqProtOutR = os.path.join(samplePartDir, 'r_%s_%s_prot.fna.gz' % (pf, ident))
fqSamOutO = os.path.join(samplePartDir, 'o_%s_%s_gmap.sam.gz' % (pf, ident))
fqSamOutR = os.path.join(samplePartDir, 'r_%s_%s_gmap.sam.gz' % (pf, ident))
fqDomOutO = os.path.join(samplePartDir, 'o_%s_%s_prot.domtblout.gz' % (pf, ident))
fqDomOutR = os.path.join(samplePartDir, 'r_%s_%s_prot.domtblout.gz' % (pf, ident))
# write FASTQ
fqOut = fq.WriteFq(fqOutO)
for e in fqContentList:
fqOut.writeFqEntry('@' + e[0], e[1], e[2], e[3])
fqOut.close()
# write PROT
fqProtOut = fq.WriteFq(fqProtOutO)
fqProtOut.write('\n'.join(map(lambda x: '>%s\n%s' % (x[0], x[1]), fqProtOutDict[famName])) + '\n')
fqProtOut.close()
# write SAM
if considerSam:
fqSamOut = fq.WriteFq(fqSamOutO)
fqSamOut.write('\n'.join(fqSamOutDict[famName]) + '\n')
fqSamOut.close()
# write DOM
fqDomOut = fq.WriteFq(fqDomOutO)
fqDomOut.write('\n'.join(fqDomOutDict[famName]) + '\n')
fqDomOut.close()
# shuffle file entries (to remove any bias imposed by the ordering)
rand.shuffleLines(fqOutO, fqOutR, 4, shuffleRandSeed)
rand.shuffleLines(fqProtOutO, fqProtOutR, 2, shuffleRandSeed)
rand.shuffleLines(fqDomOutO, fqDomOutR, 1, shuffleRandSeed)
if considerSam:
if joinedReads:
rand.shuffleLines(fqSamOutO, fqSamOutR, 1, shuffleRandSeed)
else:
rand.shuffleLines(fqSamOutO, fqSamOutR, 2, shuffleRandSeed)
# delete ordered files (keep only the shuffled ones)
os.remove(fqOutO)
os.remove(fqProtOutO)
if considerSam:
os.remove(fqSamOutO)
os.remove(fqDomOutO)
except Exception as e:
print('Exception in partitionReads:')
print sampleDir, scoreThreshold, accuracyThreshold, shuffleRandSeed, pfamPartitionedDir, joinedReads
print e.message
print type(e)
print e.args
raise e
