class MirrorControllerPython(MirrorController): def __init__(self): sys.path.append(os.path.dirname(__file__)) from asdk import DM super(MirrorControllerPython,self).__init__() self.mirror_id = ccfg.mirror_id #self.dm = DM(os.path.join(ccfg.dm_directory,self.mirror_id)) self.dm = DM(self.mirror_id) n_actuators_queried = int( self.dm.Get('NBOfActuator') ) try: assert n_actuators_queried==ccfg.mirror_n_actuators except AssertionError as ae: print 'Number of actuator disagreement.' self.command[:] = 0.0 # this doesn't really matter def set(self,vec): self.command[:] = vec[:] def send(self): self.clip() return self.dm.Send(self.command)
class scope(QThread): def __init__(self,parent=None): super(scope, self).__init__() self.delay = 0.02 # delay in loops (StackExt) self.handleA = None self.handleB = None self.QUIT = False self.stackparams = {'Date/Time':0,'X':0,'Y':0,'Z1':0,'Z2':0,'Zstep':0,'Exposure(s)':0,'CCD Temperature':0,'Pixel Size(nm)':89,'CCD setting':'','User':''} # camera self.ccd = pyAndor_P271_slm.ccd() self.ccd.CoolerON() self.ccd.SetCoolerMode(COOLER_MODE) self.ccd.SetTemperature(TEMPERATURE_SETPOINT) self.data = N.zeros(self.ccd.image_size, dtype=N.uint16) # piezo self.zst = zstage.zstage() self.zst.setPositionf(50) self.zpos = 0 # Filter wheel self.fw = filterWheel.filter_wheel() # deformable mirror self.serialName = 'BAX228' self.dm = DM( self.serialName ) self.nbAct = int( self.dm.Get('NBOfActuator') ) # self.values = [0.] * self.nbAct # self.dm.Send( self.values ) with open(FLAT_FILE, "r") as file: self.dmfile = eval(file.readline()) if (all(i <= 1.0 for i in self.dmfile)): self.dm.Send(self.dmfile) else: raise Exception(' Error: push value greater than 1.0 ') #motorized polarizer print('Initializing Polarizer') self.pol = mp2.A8MRU() self.pol.MotorOn() self.pol.setSpeed(1000,1) self.pol_array = N.array([50.,20.,-10.]) #3D print(self.pol.getState()) # priorxy self.prior = priorxyP27.prior() self.xpos = 0 self.ypos = 0 self.prior.limits=[-10000, 10000, -10000, 10000] # qxga qx.initiate() qx.open_usb_port() #Coherent 647 laser try: self.ll647 = Coherent_P33.obis('COM16') self.ll647.SetLaserOn() self.ll647.SetDigitalModMode() except: self.ll647 = None print('647nm Laser not on') #raise Exception('647nm Laser not on') #set save path t=time.localtime() self.is_videomode = False dpth=str(int(t[0]*1e4+t[1]*1e2+t[2]))+'_'+getuser() self.path=os.path.join(datapath,dpth) try: os.mkdir(self.path) except: print('Directory already exists') # defaults self.coord=(200,400,200,400) self.Name='default' ### self.Name = 'default' self.coordVal = self.ccd.GetImageCoordinates() self.old_Cval = self.ccd.GetImageCoordinates() self.normal_mode = False def __del__(self): if (self.handleA != None): daq.CCDTrig_close(self.handleA,self.handleB) x = self.dm.Reset() if (x==0): print('DM Reset') else: print('DM cannot reset') qx.close() # self.dm.close() # def get_img(self): daq.CCDTrig_run(self.handleA,self.handleB) self.ccd.WaitForNewData() self.data[:,:] = self.ccd.images def open_Acq(self,exposure=0.1,emgain=200,laser=False,Rlaser=False,cntLaser=False,LED12=1,FTM=False,conv=False,ccd=True,trig=1,regen=False): # setup camera self.ccd.SetTriggerMode(trig) #0:internal, 1:External self.ccd.SetShutterMode(2) #auto self.ccd.SetReadMode(4) #image self.ccd.SetADChannel(0) # 14-bit channel self.ccd.SetOutputAmplifier(int(conv)) self.ccd.SetHSSpeed(0) self.ccd.SetEMCCDGain(emgain) if FTM: # Frame transfer mode self.ccd.SetExposureTime(0) self.ccd.SetFrameTransferMode(1) self.ccd.SetAcquisitionMode(7) # run until abort else: self.ccd.SetExposureTime(exposure) self.ccd.SetFrameTransferMode(0) self.ccd.SetAcquisitionMode(5) # run until abort q,w,e = self.ccd.GetAcquisitionTimings() # setup triggers if not self.handleA == None: daq.CCDTrig_close(self.handleA,self.handleB) if cntLaser: digsig = self.getTiming3(FREQ,1e3*exposure,1e3*exposure,1,laser,Rlaser,LED=LED12,CCD=ccd) elif regen: digsig = self.getTiming2(FREQ,1e3*exposure,1e3*exposure,1,laser,Rlaser,LED=LED12,CCD=ccd) else: digsig = self.getTiming(FREQ,1e3*exposure,1e3*exposure,1,laser,Rlaser,LED=LED12,CCD=ccd) b = daq.CCDTrig_open(FREQ,digsig) self.handleA = b[0] self.handleB = b[1] xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] self.data = N.zeros((xs,ys), dtype=N.uint16) self.ccd.SetShutterMode(1) self.ccd.Acquire() #self.slm.SLM_on() print(q,w,e) return (q,w,e) def close_Acq(self): self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) return True def run(self): self.is_videomode = True if (self.normal_mode==True): while (self.is_videomode): self.get_img() self.emit(SIGNAL('update')) else: while (self.is_videomode): #self.slm.show_next_patt() # psz = qx.getordernum() psz =15 phs = int(psz/3) for m in range(3): self.pol.MoveAbs(self.pol_array[m]) time.sleep(0.02) for n in range(phs): qx.selecteorder(m*phs+n) qx.activate() self.get_img() self.emit(SIGNAL('update')) qx.deactivate() self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) #self.slm.SLM_off() ############## External ###################################################### def StackExt(self,start,stop,step=0.2,verbose=True): init_loc=self.zst.getPosition() no = int((stop-start)/step)+1 pos = start xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] self.data = N.zeros((no,xs,ys), N.uint16) self.ccd.SetShutterMode(1) q = self.ccd.Acquire() time.sleep(0.2) # was 0.05 for p in range(no): self.zst.setPositionf(pos) daq.CCDTrig_run(self.handleA,self.handleB) q = self.ccd.WaitForNewData() print(p,q) self.data[p] = self.ccd.images pos += step time.sleep(self.delay) self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) if verbose: T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) cur_pos = self.prior.getPosition() self.stackTags(cur_pos[0],cur_pos[1],start,stop,step,function='Z-Stack') self.zst.setPositionf(init_loc) return True def Stack_Patterns(self,start,stop,step=0.2, verbose=True): rots = self.pol_array no = int((stop-start)/step)+1 pos = start xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] # psz = qx.getordernum() psz = 15 phs = int(psz/3) self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16) # self.slm.SLM_on() self.ccd.SetShutterMode(1) q = self.ccd.Acquire() time.sleep(0.1) # was 0.2, changed 20141114 for p in range(no): self.zst.setPositionf(pos) for w in range(3): self.pol.MoveAbs(rots[w]) time.sleep(0.4) for m in range(phs): #self.pr.setVoltage(rots[m]) qx.selecteorder(phs*w+m) qx.activate() time.sleep(0.02) #print self.pr.getVoltage() #self.dmd.set_image(patt) #self.slm.show_next_patt() #self.slm.show_patt(m) daq.CCDTrig_run(self.handleA,self.handleB) q = self.ccd.WaitForNewData() print (p,q) self.data[psz*p + 5*w + m] = self.ccd.images qx.deactivate() # time.sleep(0.02) pos += step self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) # self.slm.SLM_off() #self.pr.setVoltage(0.0) if verbose: T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) cur_pos = self.zst.getPosition() self.stackTags(cur_pos,start,stop,step,function='Z-Stack patterns') return True def Stack_Sectioning(self,start,stop,step=0.2, verbose=True): no = int((stop-start)/step)+1 pos = start xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] # psz = qx.getordernum() psz = 3 self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16) self.ccd.SetShutterMode(1) q = self.ccd.Acquire() self.pol.MoveAbs(0) time.sleep(0.4) for p in range(no): self.zst.setPositionf(pos) for m in range(psz): qx.selecteorder(15+m) qx.activate() time.sleep(0.02) daq.CCDTrig_run(self.handleA,self.handleB) q = self.ccd.WaitForNewData() print (p,q) self.data[psz*p + m] = self.ccd.images qx.deactivate() time.sleep(0.02) pos += step self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) if verbose: T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) cur_pos = self.zst.getPosition() self.stackTags(cur_pos,start,stop,step,function='Z-Stack patterns') return True def Image_Patterns(self, angle=0, no=200, pol=0, verbose=True): pos = self.zst.getPosition() xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] # psz = self.slm.ni psz = 5 self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16) # self.slm.SLM_on() self.ccd.SetShutterMode(1) q = self.ccd.Acquire() time.sleep(0.01) # was 0.2, changed 20141114 self.zst.setPositionf(pos) for p in range(no): for m in range(angle*5,psz+angle*5): #self.pr.setVoltage(rots[m]) self.pol.MoveAbs(pol) qx.selecteorder(m) qx.activate() #time.sleep(.50) #print self.pr.getVoltage() #self.dmd.set_image(patt) #self.slm.show_next_patt() # self.slm.show_patt(m) daq.CCDTrig_run(self.handleA,self.handleB) q = self.ccd.WaitForNewData() print (p,q) self.data[psz*p+m%5] = self.ccd.images qx.deactivate() time.sleep(self.delay) self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) # self.slm.SLM_off() #self.pr.setVoltage(0.0) if verbose: T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) cur_pos = self.prior.getPosition() self.stackTags(cur_pos[0],cur_pos[1],function='Z-Stack patterns') return True def si_2d_pattern(self,start,stop,step=0.2, verbose=True): rots = self.pol_array no = int((stop-start)/step)+1 pos = start xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] # psz = qx.getordernum() psz = 9 phs = int(psz/3) self.data = N.zeros((psz*no,xs,ys), dtype=N.uint16) # self.slm.SLM_on() self.ccd.SetShutterMode(1) q = self.ccd.Acquire() time.sleep(0.1) # was 0.2, changed 20141114 for p in range(no): self.zst.setPositionf(pos) for w in range(3): self.pol.MoveAbs(rots[w]) time.sleep(0.8) for m in range(phs): #self.pr.setVoltage(rots[m]) qx.selecteorder(19+phs*w+m) qx.activate() time.sleep(0.02) #print self.pr.getVoltage() #self.dmd.set_image(patt) #self.slm.show_next_patt() #self.slm.show_patt(m) daq.CCDTrig_run(self.handleA,self.handleB) q = self.ccd.WaitForNewData() print (p,q) self.data[psz*p + 5*w + m] = self.ccd.images qx.deactivate() # time.sleep(0.02) pos += step self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) # self.slm.SLM_off() #self.pr.setVoltage(0.0) if verbose: T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) cur_pos = self.zst.getPosition() self.stackTags(cur_pos,start,stop,step,function='Z-Stack patterns') return True def setlaserpower(self,power): self.ll647.SetPowerLevel(power) out = self.ll647.GetPower() return out def singleSnapExt(self,verbose=True): xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] self.data = N.zeros((xs,ys), dtype=N.uint16) self.ccd.SetShutterMode(1) self.ccd.Acquire() time.sleep(0.2) # was 0.05 daq.CCDTrig_run(self.handleA,self.handleB) self.ccd.WaitForNewData() self.data[:,:] = self.ccd.images self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) if verbose: T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) cur_pos = self.prior.getPosition() self.stackTags(cur_pos[0],cur_pos[1],self.zst.getPosition(),0,0,function='Single snap') return True def setCCD_Conv_ext(self,exposure=0.100,laser=False,Rlaser=False,LED12=1): self.is_videMode = False # setup camera self.ccd.SetTriggerMode(1) #0: internal self.ccd.SetShutterMode(2) #0: auto self.ccd.SetFrameTransferMode(0) self.ccd.SetAcquisitionMode(5) # run until abort self.ccd.SetReadMode(4) #image self.ccd.SetADChannel(0) # 14-bit channel # this sets camera to 1.8us vs speed, and 3MHz readout which doesn't have a lot of pattern # to get rid of patterns entirely in conventional mode, go to 1Mhz readout self.ccd.SetVSSpeed(3) self.ccd.SetHSSpeed(0) self.ccd.SetOutputAmplifier(1) self.ccd.SetExposureTime(exposure) q,w,e = self.ccd.GetAcquisitionTimings() # setup triggers if not self.handleA == None: daq.CCDTrig_close(self.handleA,self.handleB) digsig = self.getTiming(FREQ,1e3*exposure,1e3*exposure,1,laser,Rlaser,LED=LED12) b = daq.CCDTrig_open(FREQ,digsig) self.handleA = b[0] self.handleB = b[1] return (q,w,e) def setCCD_EM_ext(self,exposure=0.1,emgain=200,laser=False,Rlaser=False,LED12=1): self.is_videMode = False # setup camera self.ccd.SetTriggerMode(1) #0:internal, 1:External self.ccd.SetShutterMode(2) #0: auto self.ccd.SetFrameTransferMode(0) self.ccd.SetAcquisitionMode(5) # run until abort self.ccd.SetReadMode(4) #image self.ccd.SetADChannel(0) # 14-bit channel self.ccd.SetOutputAmplifier(0) self.ccd.SetHSSpeed(0) self.ccd.SetEMCCDGain(emgain) self.ccd.SetExposureTime(exposure) q,w,e = self.ccd.GetAcquisitionTimings() # setup triggers if not self.handleA.value == 0: daq.CCDTrig_close(self.handleA,self.handleB) digsig = self.getTiming(FREQ,1e3*exposure,1e3*exposure,1,laser,Rlaser,LED=LED12) b = daq.CCDTrig_open(FREQ,digsig) self.handleA = b[0] self.handleB = b[1] return (q,w,e) def getTiming(self,freq,exposure,pulse,delay,laser=False,Rlaser=False,LED=1,CCD=True): ''' frequency is in Hertz, exposure and delay are in ms bit 1: CCD Trigger bit 2: LED pulse bit 3: Laser shutter bit 4: Red Laser bit 5: LED 1/2''' # laser shutter is active count = int(1.1*(exposure+delay)*0.001*freq) td = int(delay*0.001*freq) texp = int(exposure*0.001*freq) tpulse = int(pulse*0.001*freq) trigpulse = int(0.004*freq) p = range(count) laser_arr = N.zeros(count) led_arr = N.zeros(count) Rlaser_arr = N.zeros(count) if LED==1: LED12_arr = N.zeros(count) else: LED12_arr = N.ones(count) if laser: laser_arr = N.array([(i>td) & (i<(td+texp)) for i in p]) elif Rlaser: Rlaser_arr = N.array([(i>td) & (i<(td+texp)) for i in p]) else: led_arr = N.array([(i>td) & (i<(td+tpulse)) for i in p]) if (CCD): ccdtrig = N.array([(i<=(trigpulse)) for i in p]) else: ccdtrig = N.array([(0) for i in p]) #out = N.add(N.add(1.*ccdtrig,2.*lha),4.*shutt2).astype(N.int) ## out = (ccdtrig+2*led+4*laser).astype(N.int) out = (ccdtrig+2*led_arr+4*laser_arr+8*Rlaser_arr+16*LED12_arr).astype(N.int) return out def getTiming2(self,freq,exposure,pulse,delay,laser=False,Rlaser=False,LED=2,CCD=True): ''' Regen with every pulse with LED exposure time is for laser, followed by pulse time that is for LED. ''' # laser shutter is active count = int(1.1*(exposure+pulse+2*delay)*0.001*freq) td = int(delay*0.001*freq) texp = int(exposure*0.001*freq) tpulse = int(pulse*0.001*freq) trigpulse = int(0.004*freq) p = range(count) laser_arr = N.zeros(count) led_arr = N.zeros(count) Rlaser_arr = N.zeros(count) if LED==1: LED12_arr = N.zeros(count) else: LED12_arr = N.ones(count) if laser: laser_arr = N.array([(i>td) & (i<(td+texp)) for i in p]) elif Rlaser: Rlaser_arr = N.array([(i>td) & (i<(td+texp)) for i in p]) else: led_arr = N.array([(i>(2*td+texp)) & (i<(2*td+texp+tpulse)) for i in p]) if (CCD): ccdtrig = N.array([(i<=(trigpulse)) for i in p]) else: ccdtrig = N.array([(0) for i in p]) out = (ccdtrig+2*led_arr+4*laser_arr+8*Rlaser_arr+16*LED12_arr).astype(N.int) return out def getTiming3(self,freq,exposure,pulse,delay,laser=False,Rlaser=False,LED=1,CCD=True): ## def getTiming(self,freq,exposure,pulse,delay,laser=False): ''' frequency is in Hertz, exposure and delay are in ms bit 1: CCD Trigger bit 2: LED pulse bit 3: Laser shutter Contineous bit 4: Red Laser contineous bit 5: LED 1/2''' # laser shutter is active count = int(1.1*(exposure+delay)*0.001*freq) td = int(delay*0.001*freq) texp = int(exposure*0.001*freq) tpulse = int(pulse*0.001*freq) trigpulse = int(0.004*freq) p = range(count) led_arr = N.zeros(count) ccdtrig = N.zeros(count) laser_arr = N.zeros(count) Rlaser_arr = N.zeros(count) LED12_arr = N.ones(count) if LED==1: LED12_arr = N.zeros(count) if laser: laser_arr = N.ones(count) if Rlaser: Rlaser_arr = N.ones(count) if not (laser | Rlaser): # led_arr = N.array([(i>td) & (i<(td+tpulse)) for i in p]) led_arr = N.ones(count) if (CCD): ccdtrig = N.array([(i<=(trigpulse)) for i in p]) out = (ccdtrig+2*led_arr+4*laser_arr+8*Rlaser_arr+16*LED12_arr).astype(N.int) return out ####### Internal Trigger ################################################### def setCCD_Conv_Int(self,exposure=0.100): self.ccd.SetAcquisitionMode(1) # single exposure self.ccd.SetOutputAmplifier(1) self.ccd.SetTriggerMode(0) #0: internal self.ccd.SetShutterMode(2) #0: auto self.ccd.SetExposureTime(exposure) q = self.ccd.GetAcquisitionTimings() return q def StackInt(self,start,stop,step=0.2): no = int((stop-start)/step)+1 pos = start xs = self.ccd.image_size[0] ys = self.ccd.image_size[1] self.data = N.zeros((no,xs,ys), dtype=N.uint16) self.ccd.SetShutterMode(1) for p in range(no): self.zst.setPositionf(pos) q = self.ccd.Acquire() q = self.ccd.WaitForNewData() q = self.ccd.AbortAcquisition() self.data[p] = self.ccd.images pos += step self.ccd.AbortAcquisition() self.ccd.SetShutterMode(2) cur_pos = self.prior.getPosition() self.stackTags(cur_pos[0],cur_pos[1],start,stop,step,function='Z-Stack') T.imshow(self.data, vmin=self.data.min(), vmax=self.data.max()) return True ########################################################################### def saveTifA(self,slideName='',comments='',Upload=False): t=time.localtime() x = N.array([1e4,1e2,1]) t1 = int((t[0:3]*x).sum()) t2 = int((t[3:6]*x).sum()) if slideName=='': slideName=self.Name else: self.Name=slideName self.stackparams['Comments']=comments self.stackparams['Slide Name']=slideName fn = "%s-%s_%s_%s" %(t1,t2,slideName,comments) fn1 = os.path.join(self.path,fn+'.tif') fn2 = os.path.join(self.path,fn+'_ps.txt') T.imsave(fn1,self.data) self._SaveText(fn2) return fn def saveTiff(self,comments='',fn=None,Upload=False): if fn==None: return None t = fn.partition('.') fn1 = t[0]+'.tif' fn2 = t[0]+'_ps.txt' T.imsave(fn1,self.data) if comments: self.stackparams['Comments']=comments self._SaveText(fn2) return fn def _SaveText(self,fn=None): if fn==None: return False s = [] for parts in self.stackparams: s.append('%s : %s \n' % (parts, self.stackparams[parts])) s.sort() fid = open(fn,'w') fid.writelines(s) fid.close() return True def stackTags(self,xx,yy,z1,z2,zs,function='',ps=89): '''Date/Time,X,Y,Z1,Z2,Zstep,Exposure(s),CCD Temperature, Pixel Size(nm),CCD setting',User ''' self.stackparams.clear() self.stackparams['00 function']=function self.stackparams['01 Date/Time']=time.asctime() self.stackparams['05 X']=xx self.stackparams['06 Y']=yy self.stackparams['07 Z1']=z1 self.stackparams['08 Z2']=z2 self.stackparams['09 Zstep']=zs self.stackparams['02 Exposure(s)']=self.ccd.GetExposureTime() self.stackparams['03 CCD Temperature']=self.ccd.GetTemperature() self.stackparams['10 User']=getuser() self.stackparams['11 Coordinates']=self.ccd.GetImageCoordinates() self.stackparams['12 Pixel size']=ps ccdsett=self.ccd.SettingsString() i=13 for item in ccdsett.splitlines(): try: csi,csv=item.split(':') ncsi=str(i)+' '+csi self.stackparams[ncsi]=csv except: csi=0 i+=1 return True def setCoord(self,xs,xe,ys,ye): '''val=(xs,xe,ys,ye)''' oxs,oxe,oys,oye = self.coordVal self.old_Cval = (oxs,oxe,oys,oye) self.coordVal=(xs,xe,ys,ye) q=self.ccd.SetImageCoordinates(self.coordVal) return q def setCoord2(self,xs,ys,nn): '''Gets one corner (xs,ys) to make (nn x nn) image size''' oxs,oxe,oys,oye = self.coordVal self.old_Cval = (oxs,oxe,oys,oye) self.coordVal=(xs,xs+nn-1,ys,ys+nn-1) q=self.ccd.SetImageCoordinates(self.coordVal) return q def reSetCoord(self,mode = 0): '''mode 0: Full CCD:(1,512,1,512) mode 1: return to previous coordinates: old_Cval ''' if mode==0: xs,xe,ys,ye = (1,512,1,512) elif mode==1: xs,xe,ys,ye = self.old_Cval return self.setCoord(xs,xe,ys,ye)
class Form(QDialog): def __init__(self): super(Form, self).__init__() uic.loadUi('Gui_FLIR.ui', self) self.connect(self.QuitButton, SIGNAL("clicked()"), self.shutdown) self.connect(self.RunButton, SIGNAL("clicked()"), self.run) # self.connect(self.StopButton, SIGNAL("clicked()"), self.stop) self.connect(self.SaveButton, SIGNAL("clicked()"), self.Saveimg) self.connect(self.SaveWFButton, SIGNAL("clicked()"), self.savedmwf) self.connect(self.SaveZMWFButton, SIGNAL("clicked()"), self.savezmwf) self.connect(self.AutoScanButton, SIGNAL("clicked()"), self.autoscandm) self.connect(self.exposurebox, SIGNAL("returnPressed()"), self.exposureupdate) self.connect(self.thresholdbox, SIGNAL("returnPressed()"), self.thresholdupdate) self.connect(self.PushDMButton, SIGNAL("clicked()"), self.pushdm) self.connect(self.ScanZernikeModeButton, SIGNAL("clicked()"), self.scanzernikemode) self.connect(self.LoadDMButton, SIGNAL("clicked()"), self.loaddmfile) self.connect(self.InitiatePushButton, SIGNAL("clicked()"), self.set_baseimage) self.connect(self.Infl_func_pushButton, SIGNAL("clicked()"), self.influencefunction) self.ProgressBar.setRange(0, 100) self.ProgressBar.setValue(0) self.index = 0 #DM self.serialName = 'BAX228' self.dm = DM(self.serialName) self.nbAct = int(self.dm.Get('NBOfActuator')) self.values = [0.] * self.nbAct self.dm.Send(self.values) #Initial camera self.system = PySpin.System.GetInstance() self.cam_list = self.system.GetCameras() self.cam = self.cam_list[0] self.cam.Init() self.nodemap = self.cam.GetNodeMap() self.node_acquisition_mode = PySpin.CEnumerationPtr( self.nodemap.GetNode('AcquisitionMode')) self.node_acquisition_mode_continuous = self.node_acquisition_mode.GetEntryByName( 'Continuous') self.acquisition_mode_continuous = self.node_acquisition_mode_continuous.GetValue( ) self.node_acquisition_mode.SetIntValue( self.acquisition_mode_continuous) self.cam.ExposureAuto.SetValue(PySpin.ExposureAuto_Off) self.exposure_time_to_set = 200.0 self.cam.ExposureTime.SetValue(self.exposure_time_to_set) self.cam.BeginAcquisition() self.image_result = self.cam.GetNextImage() self.img = self.image_result.GetNDArray() self.image = self.img.copy() self.baseimage = self.img.copy() self.image_result.Release() self.cam.EndAcquisition() self.ah = self.CCDImage.axes.imshow(self.image) # setup the wavefront self.WFS = shwfs.Wavefront_Sensor() self.phi = self.WFS.GetAberration2img(self.baseimage, self.baseimage) self.phi = self.phi * self.WFS.Zero_Mask self.wah = self.WFimage.axes.imshow(self.phi, interpolation='nearest', vmin=-0.8, vmax=0.8) self.WFimage.figure.colorbar(self.wah) def set_baseimage(self, ): self.cam.BeginAcquisition() self.image_result = self.cam.GetNextImage() self.baseimage = self.image_result.GetNDArray().copy() self.image_result.Release() self.cam.EndAcquisition() def run(self): self.cam.BeginAcquisition() self.image_result = self.cam.GetNextImage() self.image = self.image_result.GetNDArray().copy() self.image_result.Release() self.cam.EndAcquisition() self.imageupdate() def imageupdate(self): # update ccd image self.ah.set_data(self.image) self.CCDImage.figure.canvas.draw() # measure wavefront self.phi = self.WFS.GetAberration2img(self.baseimage, self.image) self.phi = self.phi * self.WFS.Zero_Mask self.wah.set_data(self.phi) self.wah.set_clim(self.phi.min(), self.phi.max()) self.WFimage.figure.canvas.draw() self.ProgressBar.setValue(self.index) self.index = (self.index + 1) % 100 def shutdown(self): x = self.dm.Reset() if (x == 0): print('DM Reset') else: print('DM cannot reset') del self.cam self.cam_list.Clear() self.system.ReleaseInstance() self.close() def pushdm(self): push = self.PushdoubleSpinBox.value() num = self.ActspinBox.value() self.values[num] = push self.dm.Send(self.values) self.values[num] = 0. return True def influencefunction(self): n = self.nbAct push = self.PushdoubleSpinBox.value() for i in range(n): self.values[i] = push self.dm.Send(self.values) self.values[i] = 0. self.run() fn = 'act_%d' % i + '_v%f.tif' % push tf.imsave(fn, self.phi.astype(np.float32), photometric='minisblack') return True def autoscandm(self): for i in range(self.nbAct): data = [] for p in range(19): push = -0.9 + p * 0.1 self.values[i] = push self.dm.Send(self.values) self.cam.BeginAcquisition() self.image_result = self.cam.GetNextImage() self.image = self.image_result.GetNDArray().copy() self.image_result.Release() self.cam.EndAcquisition() self.phi = self.WFS.GetAberration2img(self.baseimage, self.image) self.phi = self.phi * self.WFS.Zero_Mask data[p] = self.phi self.values[i] = 0. time.sleep(0.1) fn = 'act_%d.tif' % (i) tf.imsave(join(fn), data.astype(np.float32), photometric='minisblack') return True def scanzernikemode(self): n = self.ZernikeModeBox.value() h = self.PushSpinBox.value() phiin = h * zernike.Zm(n, rad=17, orig=None, Nx=33) dmarr = 0.1 * np.dot(Sm.S, phiin.reshape(33 * 33)) dmfile = [0.] * 69 for i in range(69): dmfile[i] = dmarr[i] if (all(i <= 1.0 for i in dmfile)): self.dm.Send(dmfile) else: raise Exception(' Error: push value greater than 1.0 ') def loaddmfile(self): fns = self.dmfilebox.text() fn = r'%s' % fns with open(fn, "r") as file: cmd = eval(file.readline()) if (all(i <= 1.0 for i in cmd)): self.dm.Send(cmd) else: raise Exception(' Error: push value greater than 1.0 ') def savedmwf(self): m = self.ActspinBox.value() v = self.PushdoubleSpinBox.value() fn = 'act_%d' % m + '_v%f.tif' % v tf.imsave(fn, self.phi.astype(np.float32), photometric='minisblack') def savezmwf(self): m = self.ZernikeModeBox.value() v = self.PushSpinBox.value() fn = 'zernike_mode%d' % m + '_v%f.tif' % v tf.imsave(fn, self.phi.astype(np.float32), photometric='minisblack') def Saveimg(self): tf.imsave('camera_image.tif', self.img.astype(np.float32), photometric='minisblack') tf.imsave('wfs_image.tif', self.phi.astype(np.float32), photometric='minisblack') def updatespacing(self): text = self.Spacinglinebox.text() self.px_spacing = float(text) def updateXcenter(self): xcenter = self.xCenterbox.text() self.x_center = float(xcenter) def updateYcenter(self): ycenter = self.yCenterbox.text() self.y_center = float(ycenter) def exposureupdate(self): exposure = self.exposurebox.text() exposure = int(exposure) self.cam.ExposureTime.SetValue(exposure) def thresholdupdate(self): threshold = self.thresholdbox.text() self.theshold = float(threshold) def high_pass_filter(self, data): y, x = data.shape data[data < self.threshold] = 0.0 return data