def test_single_well(self):
p = Protocol()
test_plate = p.ref("test", None, "96-flat", discard=True)
p.absorbance(test_plate, test_plate.well(0), "475:nanometer",
"test_reading")
self.assertTrue(isinstance(p.instructions[0].wells, list))
}, {
"column": 10,
"volume": "110:microliter"
}, {
"column": 11,
"volume": "120:microliter"
}])
p.seal(test_plate)
p.thermocycle(
test_plate,
[{
"cycles": 1,
"steps": [{
"temperature": "95:celsius",
"duration": "30:minute",
}]
}])
p.unseal(test_plate)
p.image_plate(test_plate, mode="top", dataref="test_plate_result")
p.absorbance(test_plate,
sample_plate.wells_from(0, 12),
"600:nanometer",
"test_reading",
flashes=50)
#p.unseal(test_plate)
print(json.dumps(p.as_dict(), indent=2))
protocol.transfer(nc_cells.well("A1"), growth_plate.well("H1"),"30:microliter", mix_after=True)
# Step 5 - Add the engineered cells to the measurement plate
protocol.distribute(growth_plate.well("A1"), measurement_plate.wells_from("A1", 3, columnwise=False), "100:microliter")
# Step 6 - Add the negative control cells to the measurement plate
protocol.distribute(growth_plate.well("H1"), measurement_plate.wells_from("C1", 3, columnwise=False), "100:microliter")
# Step 7 - Add LB medium to the measurement plate
protocol.distribute(lb_medium_c.well("A1"), measurement_plate.wells_from("E1", 3, columnwise=False), "100:microliter")
# Step 8 - Cover the growth plate
protocol.cover(growth_plate, lid="standard")
# Step 9 - Measure indirectly cell concentrations
protocol.absorbance(measurement_plate, ["A1", "A2", "A3", "C1", "C2", "C3", "E1", "E2", "E3",], "600:nanometer","Before Incubation")
# Step 10 - Cover the measurement plate
protocol.cover(measurement_plate, lid="standard")
# Step 11 - Grow new engineered and negative control cells
protocol.incubate(growth_plate, "warm_37", "5:hour", shaking=True)
# Step 12 - Store the measurement plate in the fridge
# This step is being run in series instead of parallel. Will uncomment it when it runs in parallel.
# The measurement plate remains covered and in room temperature while the growth plate is in the incubator.
#protocol.incubate(measurement_plate, "cold_4", "4:hour")
# Step 13 - Uncover the growth plate
protocol.uncover(growth_plate)