def test_basic():
to_amplify = 'atgtctaaaggtgaagaattattcactggtgttgtcccaatgctgctggtattacc' + \
'catggtattgatgaattgtacaaatag'
template = DNA(to_amplify)
forward, reverse = design.primers(template)
amplicon = reaction.pcr(template, forward, reverse)
assert_equal(amplicon, template)
def test_over_origin():
current_path = os.path.dirname(__file__)
template = seqio.read_dna(os.path.join(current_path,
"pMODKan-HO-pACT1GEV.ape"))
assert_true(template.topology == "circular")
primer1 = design.primer(template[-200:])
primer2 = design.primer(template.reverse_complement()[-200:])
over_origin = reaction.pcr(template, primer1, primer2)
expected = template[-200:] + template[0:200]
assert_equal(str(over_origin), str(expected))