def read_self_mate_map_data_and_trim(cursor,conn,sample,species,DB_NAME,tablename,table_raw):
conn1=mb.connect(host="localhost",user="******",passwd="123456",db=DB_NAME)
cursor1 = conn1.cursor()
files = ['self_refseq','mate_refseq']
rec = ['S_R','M_R']
for index,filename in enumerate(files):
values = []
cmd = 'samtools view -F 4 '+d00.get_sample_file(cursor,sample,filename)
p1 = subprocess.Popen(cmd,shell = True,stdout=subprocess.PIPE)
for line in p1.stdout:
t = str.split(line)
values.append((t[0],rec[index],'T','101M'))
cursor1.executemany("insert into "+tablename+" values(%s,%s,%s,1000,%s) ",values);
conn1.commit()
files = ['mate_genome']
rec = ['M_G']
for index,filename in enumerate(files):
values = []
cmd = 'samtools view -F 4 '+d00.get_sample_file(cursor,sample,filename)
p1 = subprocess.Popen(cmd,shell = True,stdout=subprocess.PIPE)
for line in p1.stdout:
t = str.split(line)
if species == 'mm10':
t[2] = 'chr'+t[2]
if len(t[5])>20:
t[5] = t[5][0:20]
values.append((t[0],rec[index],t[2],t[3],t[5]))
cursor1.executemany("insert into "+tablename+" values(%s,%s,%s,%s,%s) ",values);
conn1.commit()
def FPKM_DB(cursor, conn, genes_table, samples, intype, insert, tablename):
sql = 'create table %s select * from %s' % (tablename, genes_table)
genes = d00.table_2_dict(cursor, genes_table, ['gene', 'gene'])
try:
cursor.execute(sql)
conn.commit()
cursor.execute("create index reci on " + tablename + "(gene);")
conn.commit()
except:
print "exists"
for sample in samples:
exp = d00.get_sample_info(cursor, sample, 'exp') + insert
try:
cursor.execute("alter table " + tablename + " add " + exp +
" float DEFAULT '0'")
conn.commit()
except:
print "EXISTS",
fpkm = []
f = open(d00.get_sample_file(cursor, sample, intype))
f.readline()
print d00.get_sample_file(cursor, sample, intype)
for line in f:
t = re.split('\s+', line)
if t[9] > 0 and t[0] in genes:
fpkm.append([t[9], t[0]])
cursor.executemany(
"update " + tablename + " set " + exp + "=%s where gene = %s",
fpkm)
conn.commit()
def FPKM_DB(cursor,conn,genes_table,samples,intype,insert,tablename):
sql = 'create table %s select * from %s' %(tablename,genes_table)
genes = d00.table_2_dict(cursor,genes_table,['gene','gene'])
try:
cursor.execute(sql)
conn.commit()
cursor.execute("create index reci on "+tablename+"(gene);")
conn.commit()
except:
print "exists"
for sample in samples:
exp = d00.get_sample_info(cursor,sample,'exp')+insert
try:
cursor.execute("alter table "+tablename+" add "+exp+" float DEFAULT '0'")
conn.commit()
except:
print "EXISTS",
fpkm = []
f = open(d00.get_sample_file(cursor,sample,intype))
f.readline()
print d00.get_sample_file(cursor,sample,intype)
for line in f:
t = re.split('\s+',line)
if t[9] > 0 and t[0] in genes:
fpkm.append([t[9],t[0]])
cursor.executemany("update "+tablename+" set "+exp+"=%s where gene = %s",fpkm)
conn.commit()
def BOWTIE_alignment(cursor,conn,samples,species,ref,ins,outdir,rec):
cmds = []
for sample in samples:
path = outdir+'/BAM.anchor_genome.'+sample+'_'+rec+'.bam'
fq1 = d00.get_sample_file(cursor,sample,ins[0])
fq2 = d00.get_sample_file(cursor,sample,ins[1])
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/BOWTIE.pair.sh %s %s %s %s' %(fq1,fq2,path[:-4],refall[species]['bowtie2'][ref])
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
conn.commit()
if not os.path.exists(path):
cmds.append(cmd)
return cmds
def BOWTIE_alignment(cursor,conn,samples,species,in1,in2,rec,para,outdir):
cmds = []
for sample in samples:
outsam = outdir+'/SAM.anchor.'+sample+'_'+rec+'.sam'
path = outdir+'/BAM.anchor_genome.'+sample+'_'+rec+'.bam'
fq1 = d00.get_sample_file(cursor,sample,in1)
fq2 = d00.get_sample_file(cursor,sample,in2)
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/cRNA/BOWTIE.pair.sh %s %s %s %s %s %s' %(fq1,fq2,outsam,path[:-4],Ref[species]['bowtie2']['genome'],para)
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
conn.commit()
if not os.path.exists(path):
cmds.append(cmd)
return cmds
def BWA_PAIRED(cursor,conn,specise,ref,samples,intype,folder,rec):
cmds = []
for sample in samples:
insert = rec+'_'+sample
fq1 = d00.get_sample_file(cursor,sample,intype[0])
fq2 = d00.get_sample_file(cursor,sample,intype[1])
path = folder+'/BAM'+insert+'.bam'
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/BWA.pair.sh ' +folder+' '+fq1+' '+fq2\
+' '+refall[specise]['bwa'][ref]+" "+path[:-4]+" "+insert
cmd = add_files(cmd,"/data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/BWA.pair.sh")
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s) ",[sample,rec,path,cmd])
cmds.append(cmd)
conn.commit()
return cmds
def BOWTIE_PAIRED(cursor,conn,samples,species,ref,ins,outdir,usage,rec,server="TANG"):
cmds = []
for sample in samples:
path = outdir+'/BAM.'+rec+'_'+sample+'.bam'
fq1 = d00.get_sample_file(cursor,sample,ins[0])
fq2 = d00.get_sample_file(cursor,sample,ins[1])
refpath = d00.get_ref(cursor,species,'bowtie2',ref,server)
(scriptpath,scriptcmds) = d00.get_script(cursor,'bowtie_pair',server)
cmd = 'bash %s %s %s %s %s %s' %(scriptpath,fq1,fq2,path[:-4],refpath,usage)
method = cmd+" \n"+scriptcmds
cursor.execute("replace into files (sample,type,path,method,server)values(%s,%s,%s,%s,%s)",[sample,rec,path,method,server])
conn.commit()
if not os.path.exists(path):
cmds.append(cmd)
return cmds
def TOPHAT_PAIRED(cursor,conn,specise,ref,samples,intype,report,folder,rec,server="TANG"):
cmds = []
for sample in samples:
insert = rec+'_'+sample
outdir = folder+'/'+insert
fq1 = d00.get_sample_file(cursor,sample,intype[0])
fq2 = d00.get_sample_file(cursor,sample,intype[1])
path = outdir+'/BAM'+insert+'.bam'
refpath = d00.get_ref(cursor,specise,'bowtie2',ref,server)
cmd = "bash %sscripts/TOPHAT.pair.sh %s %s %s %s %s %s %s" %(dirname,outdir,fq1,fq2,refpath,path[:-4],insert,report)
method = add_files(cmd,dirname+"scripts/TOPHAT.pair.sh")
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,method])
cmds.append(cmd)
conn.commit()
return cmds
def BWA_PAIRED(cursor,conn,specise,ref,samples,intype,folder,rec,server="TANG"):
cmds = []
for sample in samples:
insert = rec+'_'+sample
fq1 = d00.get_sample_file(cursor,sample,intype[0])
fq2 = d00.get_sample_file(cursor,sample,intype[1])
path = folder+'/BAM'+insert+'.bam'
(scriptpath,scriptcmds) = d00.get_script(cursor,'bwa_pair',server)
refpath = d00.get_ref(cursor,specise,'bwa',ref,server)
cmd = "bash %s %s %s %s %s %s %s" %(scriptpath,folder,fq1,fq2,refpath,path[:-4],insert)
method = cmd+" \n"+scriptcmds
cursor.execute("replace into files (sample,type,path,method,server) values(%s,%s,%s,%s,%s) ",[sample,rec,path,method,server])
cmds.append(cmd)
conn.commit()
return cmds
def BOWTIE_alignment(cursor, conn, samples, species, ref, ins, outdir, rec):
cmds = []
for sample in samples:
path = outdir + '/BAM.anchor_genome.' + sample + '_' + rec + '.bam'
fq1 = d00.get_sample_file(cursor, sample, ins[0])
fq2 = d00.get_sample_file(cursor, sample, ins[1])
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/BOWTIE.pair.sh %s %s %s %s' % (
fq1, fq2, path[:-4], refall[species]['bowtie2'][ref])
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, cmd])
conn.commit()
if not os.path.exists(path):
cmds.append(cmd)
return cmds
def circularRNA_tophat_pair(cursor,conn,samples,species,ref,insert,out,in1,in2):
cmds = []
for sample in samples:
outdir = out+'/'+sample
if not os.path.exists(outdir):
os.mkdir(outdir)
path = outdir+'/BAM_PE_'+sample+'_'+insert+'.bam'
c = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/cRNA_TOPHAT.pair.sh '
fq1 = d00.get_sample_file(cursor,sample,in1)
fq2 = d00.get_sample_file(cursor,sample,in2)
cmd = "%s %s %s %s %s %s %s" %(c,outdir,fq1,fq2,path[:-4],insert,refall[species]['bowtie2'][ref])
if not os.path.exists(path):
cmds.append(cmd)
cursor.execute("insert ignore into files values(%s,%s,%s,NULL,%s)",[sample,'BAM_PE_CRNA_SORT',path,cmd])
conn.commit()
return cmds
def GET_BINNED_COUNTS_ALONG_GENOME_Multiple(cursor,conn,samples,bamfile,binsize,chrom_size,tablename):
print tablename,
colnames = ['chr','bin_id']+samples
types = ['varchar(20)','int']+['int']*len(samples)
print colnames,types
in05.CREATE_TABLE(cursor,tablename,colnames,types)
cursor.execute("select chr,`length` from "+chrom_size)
results = cursor.fetchall()
samfiles = []
for sample in samples:
bamname = d00.get_sample_file(cursor,sample,bamfile)
samfiles.append(pysam.Samfile(bamname, "rb"))
counts = []
for chr in results:
for pos in range(1,int(chr[1]),binsize):
bin_info = [chr[0],int(pos/binsize)]
for samfile in samfiles:
bin_info.append(samfile.count(chr[0],pos,min(pos+binsize,int(chr[1]))))
counts.append(bin_info)
up_cmd = "insert ignore into "+tablename+" values("+" %s,"*len(colnames)
up_cmd = up_cmd[0:len(up_cmd)-1]+")"
print up_cmd
cursor.executemany(up_cmd,counts)
conn.commit()
def summarize_pair_map_conditions_db(cursor,samples,dbname,tablename,in1,dist,anchor_length): cmds = [] for sample in samples: bamfile = d00.get_sample_file(cursor,sample,in1) cmd = 'python /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/cRNA/summarize_reads_db.py %s %s %s %s %s %s' %(dbname,tablename,bamfile,dist,sample,anchor_length) cmds.append(cmd) return cmds
def RPKM_DB(cursor,conn,g_t,gene_length,totalreads,samples,intype,insert,tablename):
sql = 'create table %s select * from %s' %(tablename,gene_length)
try:
cursor.execute(sql)
conn.commit()
cursor.execute("create index reci on "+tablename+"(gene);")
conn.commit()
except:
print "exists"
gt = d00.table_2_dict(cursor,g_t,['transc','gene'])
gl = d00.table_2_dict(cursor,gene_length,['gene','length'])
for sample in samples:
total = int(d00.get_sample_info(cursor,sample,totalreads))
count = {}
exp = d00.get_sample_info(cursor,sample,'exp')+insert
try:
cursor.execute("alter table "+tablename+" add "+exp+" float DEFAULT '0'")
except:
print "EXISTS_colume"
f = open(d00.get_sample_file(cursor,sample,intype))
for line in f:
t = re.split('\s+',line)
if t[0] not in gt:
continue
gene = gt[t[0]]
if gene in gl:
if gene not in count:
count[gene] = 0
count[gene] += int(t[1])
values = []
for gene in count:
values.append([float(count[gene]*1000000*1000)/(total*int(gl[gene])),gene])
print len(values),values[1]
cursor.executemany("update "+tablename+" set "+exp+"=%s where gene = %s",values)
conn.commit()
def FILES_GROUPER(cursor,conn,samples,newsName,intype,sep):
newname = ""
for sample in samples:
newname += d00.get_sample_file(cursor,sample,intype)+sep
print newname[:-1]
method = "FILES_GROUPER #"+" "+intype
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[newsName,intype,newname,method])
conn.commit()
def circularRNA_tophat_pair(cursor,conn,samples,species,ref,out,in1,in2,rec,server="TANG"):
cmds = []
for sample in samples:
outdir = out+'/'+rec+"_"+sample
if not os.path.exists(outdir):
os.mkdir(outdir)
path = outdir+'/'+rec+"_"+sample+'.bam'
c = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/cRNA_TOPHAT.pair.sh '
fq1 = d00.get_sample_file(cursor,sample,in1)
fq2 = d00.get_sample_file(cursor,sample,in2)
refpath = d00.get_ref(cursor,species,'bowtie2',ref,server)
cmd = "%s %s %s %s %s %s %s" %(c,outdir,fq1,fq2,path[:-4],rec,refpath)
if not os.path.exists(path):
cmds.append(cmd)
cursor.execute("insert ignore into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
conn.commit()
return cmds
def BOWTIE_alignment(cursor, conn, samples, species, in1, in2, rec, para,
outdir):
cmds = []
for sample in samples:
outsam = outdir + '/SAM.anchor.' + sample + '_' + rec + '.sam'
path = outdir + '/BAM.anchor_genome.' + sample + '_' + rec + '.bam'
fq1 = d00.get_sample_file(cursor, sample, in1)
fq2 = d00.get_sample_file(cursor, sample, in2)
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/cRNA/BOWTIE.pair.sh %s %s %s %s %s %s' % (
fq1, fq2, outsam, path[:-4], Ref[species]['bowtie2']['genome'],
para)
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, cmd])
conn.commit()
if not os.path.exists(path):
cmds.append(cmd)
return cmds
def summarize_pair_map_conditions_db(cursor, samples, dbname, tablename, in1,
dist, anchor_length):
cmds = []
for sample in samples:
bamfile = d00.get_sample_file(cursor, sample, in1)
cmd = 'python /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/cRNA/summarize_reads_db.py %s %s %s %s %s %s' % (
dbname, tablename, bamfile, dist, sample, anchor_length)
cmds.append(cmd)
return cmds
def BAM_FLAGSTAT(cursor,conn,samples,intype,folder,rec):
cmds = []
for sample in samples:
path = folder+'/flagstat.'+sample+"_"+intype+'.txt'
f1 = d00.get_sample_file(cursor,sample,intype)
cmd = 'samtools flagstat '+f1+' > '+path
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
cmds.append(cmd)
conn.commit()
return cmds
def circularRNA_tophat_pair(cursor, conn, samples, species, ref, insert, out,
in1, in2):
cmds = []
for sample in samples:
outdir = out + '/' + sample
if not os.path.exists(outdir):
os.mkdir(outdir)
path = outdir + '/BAM_PE_' + sample + '_' + insert + '.bam'
c = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/cRNA_TOPHAT.pair.sh '
fq1 = d00.get_sample_file(cursor, sample, in1)
fq2 = d00.get_sample_file(cursor, sample, in2)
cmd = "%s %s %s %s %s %s %s" % (c, outdir, fq1, fq2, path[:-4], insert,
refall[species]['bowtie2'][ref])
if not os.path.exists(path):
cmds.append(cmd)
cursor.execute("insert ignore into files values(%s,%s,%s,NULL,%s)",
[sample, 'BAM_PE_CRNA_SORT', path, cmd])
conn.commit()
return cmds
def BWA_PAIRED(cursor, conn, specise, ref, samples, intype, folder, rec):
cmds = []
for sample in samples:
insert = rec + '_' + sample
fq1 = d00.get_sample_file(cursor, sample, intype[0])
fq2 = d00.get_sample_file(cursor, sample, intype[1])
path = folder + '/BAM' + insert + '.bam'
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/BWA.pair.sh ' +folder+' '+fq1+' '+fq2\
+' '+refall[specise]['bwa'][ref]+" "+path[:-4]+" "+insert
cmd = add_files(
cmd,
"/data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/BWA.pair.sh"
)
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s) ",
[sample, rec, path, cmd])
cmds.append(cmd)
conn.commit()
return cmds
def SUMMARIZE(cursor,conn,samples,intype,folder,para,rec):
cmds = []
for sample in samples:
insert = rec+'_'+sample
bam = d00.get_sample_file(cursor,sample,intype)
path = folder+'/summarize.'+insert+'.txt'
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/SUMMARIZE.sh '+bam+' '+path+' '+para
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
cmds.append(cmd)
conn.commit()
return cmds
def MAPPED_SINGLE(cursor,conn,samples,bamtype,folder,rec):
cmds = []
for sample in samples:
bam = d00.get_sample_file(cursor,sample,bamtype)
path = folder+'/'+rec+'_'+sample+'.fa.gz'
cmd = 'python /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/assembly/mapped_reads_single.py ' +bam+' '+path+' '+sample
method = add_files(cmd,"/data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/assembly/mapped_reads_single.py")
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,method])
cmds.append(cmd)
conn.commit()
return cmds
def BAM_FLAGSTAT(cursor, conn, samples, intype, folder, rec):
cmds = []
for sample in samples:
path = folder + '/flagstat.' + sample + "_" + intype + '.txt'
f1 = d00.get_sample_file(cursor, sample, intype)
cmd = 'samtools flagstat ' + f1 + ' > ' + path
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, cmd])
cmds.append(cmd)
conn.commit()
return cmds
def SUMMARIZE(cursor,conn,samples,intype,folder,para,rec,server="TANG"):
cmds = []
for sample in samples:
insert = rec+'_'+sample
bam = d00.get_sample_file(cursor,sample,intype)
path = folder+'/summarize.'+insert+'.txt'
(scriptpath,scriptcmds) = d00.get_script(cursor,'summarize',server)
cmd = 'bash %s %s %s %s' %(scriptpath,bam,path,para)
cursor.execute("replace into files (sample,type,path,method,server)values(%s,%s,%s,%s,%s)",[sample,rec,path,cmd,server])
cmds.append(cmd)
conn.commit()
return cmds
def Read_BWA_flagstat(cursor,conn,samples,intype,recs):
d02.check_table_colume(cursor,conn,'samples',recs[0],'INT')
d02.check_table_colume(cursor,conn,'samples',recs[1],'INT')
for sample in samples:
f1 = open(d00.get_sample_file(cursor,sample,intype))
a = re.split('\s+',f1.readline())[0]
f1.readline()
b = re.split('\s+',f1.readline())[0]
sql = "update %s set %s=%s,%s=%s where sample='%s'" %('samples',recs[0],a,recs[1],b,sample)
print sql
cursor.execute(sql)
conn.commit()
def read_VCF_file_single(cursor, conn, DB_NAME, tablename, samples, type):
limit = 30
sample_infos = ''
for sample in samples:
sample_info = " %s_DP varchar(5) DEFAULT '0',%s_alt float DEFAULT '0'," % (
sample, sample)
sample_infos += sample_info
sql = """CREATE TABLE %s (
`chr` varchar(20) NOT NULL DEFAULT '',
`pos` int(11) NOT NULL DEFAULT '0',
`Ref` varchar(30) DEFAULT NULL,
`Alt` varchar(30) NOT NULL DEFAULT '',
%s
PRIMARY KEY (`chr`,`pos`,`Alt`)
) ENGINE=InnoDB DEFAULT CHARSET=latin1""" % (tablename, sample_infos)
print sql
try:
cursor.execute(sql)
except:
print "EXISTS"
for sample in samples:
path = d00.get_sample_file(cursor, sample, type)
file = open(path)
values = []
for line in file:
if re.search('#', line):
continue
t = re.split('\s*', line)
info = {}
for i in re.split(';', t[7]):
a = re.split('=', i)
if len(a) > 1:
info[a[0]] = a[1]
if 'DP4' not in info:
continue
DP4 = re.split(',', info['DP4'])
if len(t[3]) > limit:
t[3] = t[3][0:limit]
continue
if len(t[4]) > limit:
t[4] = t[4][0:limit]
continue
value = (t[0], t[1], t[3], t[4], info['DP'],
float(int(DP4[2]) + int(DP4[3])) / int(info['DP']))
values.append(value)
cmd = "insert into %s (chr,pos,Ref,Alt,%s,%s)values(%%s,%%s,%%s,%%s,%%s,%%s) on duplicate key update %s=values(%s),%s=values(%s)" % (
tablename, sample + '_DP', sample + '_alt', sample + '_DP',
sample + '_DP', sample + '_alt', sample + '_alt')
print cmd, values[0]
cursor.executemany(cmd, values)
conn.commit()
cursor.close()
conn.close()
def Read_BWA_flagstat(cursor, conn, samples, intype, recs):
d02.check_table_colume(cursor, conn, 'samples', recs[0], 'INT')
d02.check_table_colume(cursor, conn, 'samples', recs[1], 'INT')
for sample in samples:
f1 = open(d00.get_sample_file(cursor, sample, intype))
a = re.split('\s+', f1.readline())[0]
f1.readline()
b = re.split('\s+', f1.readline())[0]
sql = "update %s set %s=%s,%s=%s where sample='%s'" % (
'samples', recs[0], a, recs[1], b, sample)
print sql
cursor.execute(sql)
conn.commit()
def SUMMARIZE(cursor, conn, samples, intype, folder, para, rec):
cmds = []
for sample in samples:
insert = rec + '_' + sample
bam = d00.get_sample_file(cursor, sample, intype)
path = folder + '/summarize.' + insert + '.txt'
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/SUMMARIZE.sh ' + bam + ' ' + path + ' ' + para
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, cmd])
cmds.append(cmd)
conn.commit()
return cmds
def BWA_SINGLE(cursor,conn,specise,ref,samples,intype,folder,rec):
cmds = []
for sample in samples:
insert = rec+'_'+sample
fq = d00.get_sample_file(cursor,sample,intype)
path = folder+'/BAM'+insert+'.bam'
refpath = d00.get_ref(cursor,specise,'bwa',ref,server)
cmd = "bash %sscripts/BWA.pair.sh %s %s %s %s %s" %(dirname,folder,fq,refpath,path[:-4],insert)
method = add_files(cmd,dirname+"scripts/BWA.single.sh")
cursor.execute("insert ignore into files (sample,type,path,method)values(%s,%s,%s,%s) ",[sample,rec,path,method])
cmds.append(cmd)
conn.commit()
return cmds
def CUFFLINKS(cursor,conn,species,ref,samples,intype,folder,rec):
cmds = []
for sample in samples:
insert = rec+'_'+sample
outdir = folder+'/'+insert
if not os.path.exists(outdir):
os.mkdir(outdir)
bam = d00.get_sample_file(cursor,sample,intype)
path = outdir+'/genes.fpkm_tracking'
cmd = 'cufflinks -o %s -p 6 -G %s %s' %(outdir,refall[species]['gtf'][ref],bam)
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
cmds.append(cmd)
conn.commit()
return cmds
def TOPHAT_SINGLE(cursor,conn,specise,ref,samples,intype,folder,report,rec):
cmds = []
for sample in samples:
insert = rec+'_'+sample
outdir = folder+'/'+insert
bam = d00.get_sample_file(cursor,sample,intype)
path = outdir+'/BAM'+insert+'.bam'
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/TOPHAT.single.sh ' +outdir+' '+bam\
+' '+refall[specise]['bowtie2'][ref]+" "+path[:-4]+" "+insert+" "+report
method = add_files(cmd,"/data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/TOPHAT.single.sh")
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,method])
cmds.append(cmd)
conn.commit()
return cmds
def read_self_mate_map_data_and_trim(cursor, conn, sample, species, DB_NAME,
tablename, table_raw):
conn1 = mb.connect(host="localhost",
user="******",
passwd="123456",
db=DB_NAME)
cursor1 = conn1.cursor()
files = ['self_refseq', 'mate_refseq']
rec = ['S_R', 'M_R']
for index, filename in enumerate(files):
values = []
cmd = 'samtools view -F 4 ' + d00.get_sample_file(
cursor, sample, filename)
p1 = subprocess.Popen(cmd, shell=True, stdout=subprocess.PIPE)
for line in p1.stdout:
t = str.split(line)
values.append((t[0], rec[index], 'T', '101M'))
cursor1.executemany(
"insert into " + tablename + " values(%s,%s,%s,1000,%s) ", values)
conn1.commit()
files = ['mate_genome']
rec = ['M_G']
for index, filename in enumerate(files):
values = []
cmd = 'samtools view -F 4 ' + d00.get_sample_file(
cursor, sample, filename)
p1 = subprocess.Popen(cmd, shell=True, stdout=subprocess.PIPE)
for line in p1.stdout:
t = str.split(line)
if species == 'mm10':
t[2] = 'chr' + t[2]
if len(t[5]) > 20:
t[5] = t[5][0:20]
values.append((t[0], rec[index], t[2], t[3], t[5]))
cursor1.executemany(
"insert into " + tablename + " values(%s,%s,%s,%s,%s) ", values)
conn1.commit()
def read_VCF_file_single(cursor,conn,DB_NAME,tablename,samples,type):
limit = 30
sample_infos = ''
for sample in samples:
sample_info = " %s_DP varchar(5) DEFAULT '0',%s_alt float DEFAULT '0'," %(sample,sample)
sample_infos += sample_info
sql = """CREATE TABLE %s (
`chr` varchar(20) NOT NULL DEFAULT '',
`pos` int(11) NOT NULL DEFAULT '0',
`Ref` varchar(30) DEFAULT NULL,
`Alt` varchar(30) NOT NULL DEFAULT '',
%s
PRIMARY KEY (`chr`,`pos`,`Alt`)
) ENGINE=InnoDB DEFAULT CHARSET=latin1""" %(tablename,sample_infos)
print sql
try:
cursor.execute(sql)
except:
print "EXISTS"
for sample in samples:
path = d00.get_sample_file(cursor,sample,type)
file = open(path)
values = []
for line in file:
if re.search('#',line):
continue
t = re.split('\s*',line)
info = {}
for i in re.split(';',t[7]):
a = re.split('=',i)
if len(a)>1:
info[a[0]] = a[1]
if 'DP4' not in info:
continue
DP4 = re.split(',',info['DP4'])
if len(t[3])>limit:
t[3]=t[3][0:limit]
continue
if len(t[4])>limit:
t[4]=t[4][0:limit]
continue
value = (t[0],t[1],t[3],t[4],info['DP'],float(int(DP4[2])+int(DP4[3]))/int(info['DP']))
values.append(value)
cmd = "insert into %s (chr,pos,Ref,Alt,%s,%s)values(%%s,%%s,%%s,%%s,%%s,%%s) on duplicate key update %s=values(%s),%s=values(%s)" %(tablename,sample+'_DP',sample+'_alt',sample+'_DP',sample+'_DP',sample+'_alt',sample+'_alt')
print cmd,values[0]
cursor.executemany(cmd,values)
conn.commit()
cursor.close()
conn.close()
def MAPPED_SINGLE(cursor, conn, samples, bamtype, folder, rec):
cmds = []
for sample in samples:
bam = d00.get_sample_file(cursor, sample, bamtype)
path = folder + '/' + rec + '_' + sample + '.fa.gz'
cmd = 'python /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/assembly/mapped_reads_single.py ' + bam + ' ' + path + ' ' + sample
method = add_files(
cmd,
"/data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/assembly/mapped_reads_single.py"
)
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, method])
cmds.append(cmd)
conn.commit()
return cmds
def makes_anchors_fq(cursor,conn,samples,bamfile,outdir,length,insert,rec):
sql = []
cmds = []
for sample in samples:
anchor1 = outdir+'/Anchor.'+insert+'_'+sample+'.1.fq.gz'
anchor2 = outdir+'/Anchor.'+insert+'_'+sample+'.2.fq.gz'
if os.path.exists(anchor1):
print "ALREADY EXISTS"
else:
bam_pe = d00.get_sample_file(cursor,sample,bamfile)
cmd = 'python /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/cRNA/make_anchors.py %s %s %s %s' %(bam_pe,anchor1,anchor2,length)
cmds.append(cmd)
sql.append([sample,rec[0],anchor1,cmd])
sql.append([sample,rec[1],anchor2,cmd])
cursor.executemany("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",sql)
conn.commit()
return cmds
def CUFFLINKS(cursor, conn, species, ref, samples, intype, folder, rec):
cmds = []
for sample in samples:
insert = rec + '_' + sample
outdir = folder + '/' + insert
if not os.path.exists(outdir):
os.mkdir(outdir)
bam = d00.get_sample_file(cursor, sample, intype)
path = outdir + '/genes.fpkm_tracking'
cmd = 'cufflinks -o %s -p 6 -G %s %s' % (
outdir, refall[species]['gtf'][ref], bam)
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, cmd])
cmds.append(cmd)
conn.commit()
return cmds
def RPKM_DB(cursor, conn, g_t, gene_length, totalreads, samples, intype,
insert, tablename):
sql = 'create table %s select * from %s' % (tablename, gene_length)
try:
cursor.execute(sql)
conn.commit()
cursor.execute("create index reci on " + tablename + "(gene);")
conn.commit()
except:
print "exists"
gt = d00.table_2_dict(cursor, g_t, ['transc', 'gene'])
gl = d00.table_2_dict(cursor, gene_length, ['gene', 'length'])
for sample in samples:
total = int(d00.get_sample_info(cursor, sample, totalreads))
count = {}
exp = d00.get_sample_info(cursor, sample, 'exp') + insert
try:
cursor.execute("alter table " + tablename + " add " + exp +
" float DEFAULT '0'")
except:
print "EXISTS_colume"
f = open(d00.get_sample_file(cursor, sample, intype))
for line in f:
t = re.split('\s+', line)
if t[0] not in gt:
continue
gene = gt[t[0]]
if gene in gl:
if gene not in count:
count[gene] = 0
count[gene] += int(t[1])
values = []
for gene in count:
values.append([
float(count[gene] * 1000000 * 1000) / (total * int(gl[gene])),
gene
])
print len(values), values[1]
cursor.executemany(
"update " + tablename + " set " + exp + "=%s where gene = %s",
values)
conn.commit()
def TOPHAT_SINGLE(cursor, conn, specise, ref, samples, intype, folder, report,
rec):
cmds = []
for sample in samples:
insert = rec + '_' + sample
outdir = folder + '/' + insert
bam = d00.get_sample_file(cursor, sample, intype)
path = outdir + '/BAM' + insert + '.bam'
cmd = 'bash /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/TOPHAT.single.sh ' +outdir+' '+bam\
+' '+refall[specise]['bowtie2'][ref]+" "+path[:-4]+" "+insert+" "+report
method = add_files(
cmd,
"/data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/scripts/TOPHAT.single.sh"
)
cursor.execute(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)",
[sample, rec, path, method])
cmds.append(cmd)
conn.commit()
return cmds
def makes_anchors_fq(cursor, conn, samples, bamfile, outdir, length, insert,
rec):
sql = []
cmds = []
for sample in samples:
anchor1 = outdir + '/Anchor.' + insert + '_' + sample + '.1.fq.gz'
anchor2 = outdir + '/Anchor.' + insert + '_' + sample + '.2.fq.gz'
if os.path.exists(anchor1):
print "ALREADY EXISTS"
else:
bam_pe = d00.get_sample_file(cursor, sample, bamfile)
cmd = 'python /data/Analysis/fanxiaoying/project/project01_polyA-RNAseq/modules/cRNA/make_anchors.py %s %s %s %s' % (
bam_pe, anchor1, anchor2, length)
cmds.append(cmd)
sql.append([sample, rec[0], anchor1, cmd])
sql.append([sample, rec[1], anchor2, cmd])
cursor.executemany(
"replace into files (sample,type,path,method)values(%s,%s,%s,%s)", sql)
conn.commit()
return cmds
def COMMAND_generator(cursor,conn,samples,template,infiles,folder,suffix,rec):
cmds = []
for sample in samples:
lists = []
for i in infiles:
lists.append(d00.get_sample_file(cursor,sample,i))
path = folder+'/'+rec+'_'+sample+suffix
if suffix=='/':
path = path[:-1]
if suffix=='/' and not os.path.exists(path):
os.mkdir(path)
lists.append(path)
cmd = template
for i in range(len(lists)):
cmd = cmd.replace("#"+str(i),lists[i])
cmd = cmd.replace("#sample",sample)
if not (rec == '' or rec == 'NA'):
cursor.execute("replace into files (sample,type,path,method)values(%s,%s,%s,%s)",[sample,rec,path,cmd])
cmds.append(cmd)
conn.commit()
return cmds
def Samples_Bam_Handles(cursor,conn,samples,bamtype): bamfiles = [] for sample in samples: file_path = d00.get_sample_file(cursor,sample,bamtype) bamfiles.append(pysam.Samfile(file_path, "rb")) return bamfiles