def _add_gene_equivalencies(self, xrefs, gene_id, taxon):
"""
Add equivalentClass and sameAs relationships
Uses external resource map located in
/resources/clique_leader.yaml to determine
if an ID space is a clique leader
"""
clique_map = self.open_and_parse_yaml(self.resources['clique_leader'])
if self.testMode:
graph = self.testgraph
else:
graph = self.graph
filter_out = ['Vega', 'IMGT/GENE-DB', 'Araport']
# These will be made xrefs
taxon_spec_xref_filters = {'10090': ['ENSEMBL'], '9606': ['ENSEMBL']}
if taxon in taxon_spec_xref_filters:
taxon_spec_filters = taxon_spec_xref_filters[taxon]
else:
taxon_spec_filters = []
model = Model(graph)
# deal with the xrefs
# MIM:614444|HGNC:HGNC:16851|Ensembl:ENSG00000136828|HPRD:11479|Vega:OTTHUMG00000020696
for ref in xrefs.strip().split('|'):
xref_curie = self._cleanup_id(ref)
if xref_curie is not None and xref_curie.strip() != '':
if re.match(r'HPRD', xref_curie):
# proteins are not == genes.
model.addTriple(gene_id,
self.properties['has_gene_product'],
xref_curie)
continue
# skip some of these for now
if xref_curie.split(':')[0] in filter_out:
continue
if xref_curie.split(':')[0] in taxon_spec_xref_filters:
model.addXref(gene_id, xref_curie)
if re.match(r'^OMIM', xref_curie):
if DipperUtil.is_omim_disease(xref_curie):
continue
try:
if self.class_or_indiv.get(gene_id) == 'C':
model.addEquivalentClass(gene_id, xref_curie)
if int(taxon) in clique_map:
if clique_map[int(taxon)] == xref_curie.split(
':')[0]:
model.makeLeader(xref_curie)
elif clique_map[int(taxon)] == gene_id.split(
':')[0]:
model.makeLeader(gene_id)
else:
model.addSameIndividual(gene_id, xref_curie)
except AssertionError as e:
logger.warn("Error parsing {0}: {1}".format(gene_id, e))
return
def _add_gene_equivalencies(self, xrefs, gene_id, taxon):
"""
Add equivalentClass and sameAs relationships
Uses external resource map located in
/resources/clique_leader.yaml to determine
if an ID space is a clique leader
"""
clique_map = self.open_and_parse_yaml(self.resources['clique_leader'])
if self.testMode:
graph = self.testgraph
else:
graph = self.graph
filter_out = ['Vega', 'IMGT/GENE-DB', 'Araport']
taxon_spec_filters = {
'10090': ['ENSEMBL']
}
if taxon in taxon_spec_filters:
filter_out += taxon_spec_filters[taxon]
model = Model(graph)
# deal with the xrefs
# MIM:614444|HGNC:HGNC:16851|Ensembl:ENSG00000136828|HPRD:11479|Vega:OTTHUMG00000020696
for ref in xrefs.strip().split('|'):
xref_curie = self._cleanup_id(ref)
if xref_curie is not None and xref_curie.strip() != '':
if re.match(r'HPRD', xref_curie):
# proteins are not == genes.
model.addTriple(
gene_id,
self.properties['has_gene_product'], xref_curie)
continue
# skip some of these for now
if xref_curie.split(':')[0] in filter_out:
continue
if re.match(r'^OMIM', xref_curie):
if DipperUtil.is_omim_disease(xref_curie):
continue
try:
if self.class_or_indiv.get(gene_id) == 'C':
model.addEquivalentClass(
gene_id, xref_curie)
if int(taxon) in clique_map:
if clique_map[int(taxon)] == xref_curie.split(':')[0]:
model.makeLeader(xref_curie)
elif clique_map[int(taxon)] == gene_id.split(':')[0]:
model.makeLeader(gene_id)
else:
model.addSameIndividual(gene_id, xref_curie)
except AssertionError as e:
logger.warn("Error parsing {0}: {1}".format(gene_id, e))
return
def _add_gene_equivalencies(self, xrefs, gene_id, taxon):
"""
Add equivalentClass and sameAs relationships
Uses external resource map located in
/resources/clique_leader.yaml to determine
if an NCBITaxon ID space is a clique leader
"""
clique_map = self.open_and_parse_yaml(self.resources['clique_leader'])
if self.test_mode:
graph = self.testgraph
else:
graph = self.graph
model = Model(graph)
filter_out = ['Vega', 'IMGT/GENE-DB', 'Araport']
# deal with the dbxrefs
# MIM:614444|HGNC:HGNC:16851|Ensembl:ENSG00000136828|HPRD:11479|Vega:OTTHUMG00000020696
for dbxref in xrefs.strip().split('|'):
prefix = ':'.join(dbxref.split(':')[:-1]).strip()
if prefix in self.localtt:
prefix = self.localtt[prefix]
dbxref_curie = ':'.join((prefix, dbxref.split(':')[-1]))
if dbxref_curie is not None and prefix != '':
if prefix == 'HPRD': # proteins are not == genes.
model.addTriple(gene_id, self.globaltt['has gene product'],
dbxref_curie)
continue
# skip some of these for now based on curie prefix
if prefix in filter_out:
continue
if prefix == 'ENSEMBL':
model.addXref(gene_id, dbxref_curie)
if prefix == 'OMIM':
if DipperUtil.is_omim_disease(dbxref_curie):
continue
try:
if self.class_or_indiv.get(gene_id) == 'C':
model.addEquivalentClass(gene_id, dbxref_curie)
if taxon in clique_map:
if clique_map[taxon] == prefix:
model.makeLeader(dbxref_curie)
elif clique_map[taxon] == gene_id.split(':')[0]:
model.makeLeader(gene_id)
else:
model.addSameIndividual(gene_id, dbxref_curie)
except AssertionError as err:
LOG.warning("Error parsing %s: %s", gene_id, err)
return
def _process_genes(self, limit=None):
if self.testMode:
graph = self.testgraph
else:
graph = self.graph
geno = Genotype(graph)
model = Model(graph)
raw = '/'.join((self.rawdir, self.files['genes']['file']))
line_counter = 0
logger.info("Processing HGNC genes")
with open(raw, 'r', encoding="utf8") as csvfile:
filereader = csv.reader(csvfile, delimiter='\t', quotechar='\"')
# curl -s ftp://ftp.ebi.ac.uk/pub/databases/genenames/new/tsv/hgnc_complete_set.txt | head -1 | tr '\t' '\n' | grep -n .
for row in filereader:
(hgnc_id, symbol, name, locus_group, locus_type, status,
location, location_sortable, alias_symbol, alias_name,
prev_symbol, prev_name, gene_family, gene_family_id,
date_approved_reserved, date_symbol_changed,
date_name_changed, date_modified, entrez_id, ensembl_gene_id,
vega_id, ucsc_id, ena, refseq_accession, ccds_id, uniprot_ids,
pubmed_id, mgd_id, rgd_id, lsdb, cosmic, omim_id, mirbase,
homeodb, snornabase, bioparadigms_slc, orphanet,
pseudogene_org, horde_id, merops, imgt, iuphar,
kznf_gene_catalog, mamit_trnadb, cd, lncrnadb, enzyme_id,
intermediate_filament_db, rna_central_ids) = row
line_counter += 1
# skip header
if line_counter <= 1:
continue
if self.testMode and entrez_id != '' and \
int(entrez_id) not in self.gene_ids:
continue
if name == '':
name = None
gene_type_id = self.resolve(locus_type,
False) # withdrawn -> None?
if gene_type_id != locus_type:
model.addClassToGraph(hgnc_id, symbol, gene_type_id, name)
if locus_type == 'withdrawn':
model.addDeprecatedClass(hgnc_id)
else:
model.makeLeader(hgnc_id)
if entrez_id != '':
model.addEquivalentClass(hgnc_id, 'NCBIGene:' + entrez_id)
if ensembl_gene_id != '':
model.addEquivalentClass(hgnc_id,
'ENSEMBL:' + ensembl_gene_id)
if omim_id != '' and "|" not in omim_id:
omim_curie = 'OMIM:' + omim_id
if not DipperUtil.is_omim_disease(omim_curie):
model.addEquivalentClass(hgnc_id, omim_curie)
geno.addTaxon(self.hs_txid, hgnc_id)
# add pubs as "is about"
if pubmed_id != '':
for p in re.split(r'\|', pubmed_id.strip()):
if str(p) != '':
graph.addTriple('PMID:' + str(p.strip()),
self.globaltt['is_about'], hgnc_id)
# add chr location
# sometimes two are listed, like: 10p11.2 or 17q25
# -- there are only 2 of these FRA10A and MPFD
# sometimes listed like "1 not on reference assembly"
# sometimes listed like 10q24.1-q24.3
# sometimes like 11q11 alternate reference locus
band = chrom = None
chr_pattern = r'(\d+|X|Y|Z|W|MT)[pq$]'
chr_match = re.match(chr_pattern, location)
if chr_match is not None and len(chr_match.groups()) > 0:
chrom = chr_match.group(1)
chrom_id = makeChromID(chrom, self.hs_txid, 'CHR')
band_pattern = r'([pq][A-H\d]?\d?(?:\.\d+)?)'
band_match = re.search(band_pattern, location)
feat = Feature(graph, hgnc_id, None, None)
if band_match is not None and len(band_match.groups()) > 0:
band = band_match.group(1)
band = chrom + band
# add the chr band as the parent to this gene
# as a feature but assume that the band is created
# as a class with properties elsewhere in Monochrom
band_id = makeChromID(band, self.hs_txid, 'CHR')
model.addClassToGraph(band_id, None)
feat.addSubsequenceOfFeature(band_id)
else:
model.addClassToGraph(chrom_id, None)
feat.addSubsequenceOfFeature(chrom_id)
if not self.testMode and limit is not None and line_counter > limit:
break
# end loop through file
return
def _process_omim2gene(self, limit=None):
"""
This method maps the OMIM IDs and KEGG gene ID.
Currently split based on the link_type field.
Equivalent link types are mapped as gene XRefs.
Reverse link types are mapped as disease to gene associations.
Original link types are currently skipped.
Triples created:
<kegg_gene_id> is a Gene
<omim_gene_id> is a Gene
<kegg_gene_id>> hasXref <omim_gene_id>
<assoc_id> has subject <omim_disease_id>
<assoc_id> has object <kegg_gene_id>
:param limit:
:return:
"""
LOG.info("Processing OMIM to KEGG gene")
if self.test_mode:
graph = self.testgraph
else:
graph = self.graph
model = Model(graph)
line_counter = 0
geno = Genotype(graph)
raw = '/'.join((self.rawdir, self.files['omim2gene']['file']))
with open(raw, 'r', encoding="iso-8859-1") as csvfile:
filereader = csv.reader(csvfile, delimiter='\t', quotechar='\"')
for row in filereader:
line_counter += 1
(kegg_gene_id, omim_id, link_type) = row
if self.test_mode and kegg_gene_id not in self.test_ids['genes']:
continue
kegg_gene_id = 'KEGG-' + kegg_gene_id.strip()
omim_id = re.sub(r'omim', 'OMIM', omim_id)
if link_type == 'equivalent':
# these are genes!
# so add them as a class then make equivalence
model.addClassToGraph(omim_id, None)
geno.addGene(kegg_gene_id, None)
if not DipperUtil.is_omim_disease(omim_id):
model.addEquivalentClass(kegg_gene_id, omim_id)
elif link_type == 'reverse':
# make an association between an OMIM ID & the KEGG gene ID
# we do this with omim ids because
# they are more atomic than KEGG ids
alt_locus_id = self._make_variant_locus_id(kegg_gene_id, omim_id)
alt_label = self.label_hash[alt_locus_id]
model.addIndividualToGraph(
alt_locus_id, alt_label, self.globaltt['variant_locus'])
geno.addAffectedLocus(alt_locus_id, kegg_gene_id)
model.addBlankNodeAnnotation(alt_locus_id)
# Add the disease to gene relationship.
rel = self.globaltt['is marker for']
assoc = G2PAssoc(graph, self.name, alt_locus_id, omim_id, rel)
assoc.add_association_to_graph()
elif link_type == 'original':
# these are sometimes a gene, and sometimes a disease
LOG.info(
'Unable to handle original link for %s-%s',
kegg_gene_id, omim_id)
else:
# don't know what these are
LOG.warning(
'Unhandled link type for %s-%s: %s',
kegg_gene_id, omim_id, link_type)
if (not self.test_mode) and (
limit is not None and line_counter > limit):
break
LOG.info("Done with OMIM to KEGG gene")
return
def _process_genes(self, limit=None):
if self.testMode:
g = self.testgraph
else:
g = self.graph
geno = Genotype(g)
model = Model(g)
raw = '/'.join((self.rawdir, self.files['genes']['file']))
line_counter = 0
logger.info("Processing HGNC genes")
with open(raw, 'r', encoding="utf8") as csvfile:
filereader = csv.reader(csvfile, delimiter='\t', quotechar='\"')
# curl -s ftp://ftp.ebi.ac.uk/pub/databases/genenames/new/tsv/hgnc_complete_set.txt | head -1 | tr '\t' '\n' | grep -n .
for row in filereader:
(hgnc_id,
symbol,
name,
locus_group,
locus_type,
status,
location,
location_sortable,
alias_symbol,
alias_name,
prev_symbol,
prev_name,
gene_family,
gene_family_id,
date_approved_reserved,
date_symbol_changed,
date_name_changed,
date_modified,
entrez_id,
ensembl_gene_id,
vega_id,
ucsc_id,
ena,
refseq_accession,
ccds_id,
uniprot_ids,
pubmed_id,
mgd_id,
rgd_id,
lsdb,
cosmic,
omim_id,
mirbase,
homeodb,
snornabase,
bioparadigms_slc,
orphanet,
pseudogene_org,
horde_id,
merops,
imgt,
iuphar,
kznf_gene_catalog,
mamit_trnadb,
cd,
lncrnadb,
enzyme_id,
intermediate_filament_db,
rna_central_ids) = row
line_counter += 1
# skip header
if line_counter <= 1:
continue
if self.testMode and entrez_id != '' \
and int(entrez_id) not in self.gene_ids:
continue
if name == '':
name = None
gene_type_id = self._get_gene_type(locus_type)
model.addClassToGraph(hgnc_id, symbol, gene_type_id, name)
if locus_type == 'withdrawn':
model.addDeprecatedClass(hgnc_id)
else:
model.makeLeader(hgnc_id)
if entrez_id != '':
model.addEquivalentClass(
hgnc_id, 'NCBIGene:' + entrez_id)
if ensembl_gene_id != '':
model.addEquivalentClass(
hgnc_id, 'ENSEMBL:' + ensembl_gene_id)
if omim_id != '' and "|" not in omim_id:
omim_curie = 'OMIM:' + omim_id
if not DipperUtil.is_omim_disease(omim_curie):
model.addEquivalentClass(hgnc_id, omim_curie)
geno.addTaxon('NCBITaxon:9606', hgnc_id)
# add pubs as "is about"
if pubmed_id != '':
for p in re.split(r'\|', pubmed_id.strip()):
if str(p) != '':
g.addTriple(
'PMID:' + str(p.strip()),
model.object_properties['is_about'], hgnc_id)
# add chr location
# sometimes two are listed, like: 10p11.2 or 17q25
# -- there are only 2 of these FRA10A and MPFD
# sometimes listed like "1 not on reference assembly"
# sometimes listed like 10q24.1-q24.3
# sometimes like 11q11 alternate reference locus
band = chrom = None
chr_pattern = r'(\d+|X|Y|Z|W|MT)[pq$]'
chr_match = re.match(chr_pattern, location)
if chr_match is not None and len(chr_match.groups()) > 0:
chrom = chr_match.group(1)
chrom_id = makeChromID(chrom, 'NCBITaxon:9606', 'CHR')
band_pattern = r'([pq][A-H\d]?\d?(?:\.\d+)?)'
band_match = re.search(band_pattern, location)
f = Feature(g, hgnc_id, None, None)
if band_match is not None and len(band_match.groups()) > 0:
band = band_match.group(1)
band = chrom + band
# add the chr band as the parent to this gene
# as a feature but assume that the band is created
# as a class with properties elsewhere in Monochrom
# TEC Monoch? Monarchdom??
band_id = makeChromID(band, 'NCBITaxon:9606', 'CHR')
model.addClassToGraph(band_id, None)
f.addSubsequenceOfFeature(band_id)
else:
model.addClassToGraph(chrom_id, None)
f.addSubsequenceOfFeature(chrom_id)
if not self.testMode \
and limit is not None and line_counter > limit:
break
# end loop through file
return
