def work(self):
data = self.data
self.logger(message="Initializing...")
if data.editFeatures:
record = sequence_to_biopython_record(data.sequence.upper())
for feature in sorted(data.editedFeatures.values(),
key=lambda f: (f.start, f.end)):
annotate_record(
record,
feature_type="misc_feature",
location=(feature.start, feature.end),
label=feature.label,
)
else:
record = records_from_data_files([data.file])[0]
problem = DnaOptimizationProblem.from_record(record,
logger=self.logger)
problem.optimization_stagnation_tolerance = 30
success, summary, zip_data = problem.optimize_with_report(
target="@memory", project_name=record.id)
return {
"zip_file": {
"data": data_to_html_data(zip_data, "zip"),
"name": "optimization_report.zip",
"mimetype": "application/zip",
},
"success": success,
"summary": summary,
}
def work(self):
data = self.data
self.logger(message='Initializing...')
if data.editFeatures:
record = sequence_to_biopython_record(data.sequence.upper())
for feature in sorted(data.editedFeatures.values(),
key=lambda f: (f.start, f.end)):
annotate_record(record,
feature_type="misc_feature",
location=(feature.start, feature.end),
label=feature.label)
else:
records, fmt = records_from_data_file(data.file)
record = records[0]
problem = DnaOptimizationProblem.from_record(record)
problem.max_random_iters = 1000
problem.logger = self.logger
success, summary, zip_data = optimization_with_report(
target="@memory", problem=problem, project_name=record.id)
return {
'zip_file': {
'data': data_to_html_data(zip_data, 'zip'),
'name': 'optimization_report.zip',
'mimetype': 'application/zip'
},
'success': success,
'summary': summary
}
def __init__(
self,
name="unnamed domesticator",
left_flank="",
right_flank="",
constraints=(),
objectives=(),
cds_by_default=False,
description=None,
simultaneous_mutations=1,
minimize_edits=True,
logger=None,
):
if isinstance(left_flank, str):
left_flank = sequence_to_biopython_record(left_flank)
annotate_record(left_flank, label="left flank")
if isinstance(right_flank, str):
right_flank = sequence_to_biopython_record(right_flank)
annotate_record(right_flank, label="right flank")
self.name = name
self.constraints = constraints
self.left_flank = left_flank
self.right_flank = right_flank
self.constraints = list(constraints)
self.objectives = list(objectives)
self.description = description
self.logger = logger
self.simultaneous_mutations = simultaneous_mutations
self.minimize_edits = minimize_edits
self.cds_by_default = cds_by_default
def test_feature_to_spec():
sequence = random_dna_sequence(100)
record = sequence_to_biopython_record(sequence)
label = "@gc(40-60%/20bp) & @no(BsaI_site) & @keep"
annotate_record(record, label=label)
feature = record.features[0]
specs = Specification.list_from_biopython_feature(feature)
assert len(specs) == 3
def test_record_with_multispec_feature():
sequence = random_dna_sequence(100)
record = sequence_to_biopython_record(sequence)
label = "@gc(40-60%/20bp) & @no(BsaI_site) & @keep"
annotate_record(record, label=label)
problem = DnaOptimizationProblem.from_record(record)
assert len(problem.constraints) == 3
c1, c2, c3 = problem.constraints
assert c1.mini == 0.4
assert c2.pattern.name == "BsaI"
def test_that_constraints_in_records_are_accounted_for():
sequence = "ATACGTCTCTAG"
rec = sequence_to_biopython_record(sequence)
annotate_record(rec, label="@cds")
from genedom import BUILTIN_STANDARDS
emma = BUILTIN_STANDARDS["EMMA"]
p7 = emma.domesticators["p7"]
result = p7.domesticate(rec)
seq_after = str(
result.record_after[len(p7.left_flank):-len(p7.right_flank)].seq)
assert translate(seq_after) == translate(sequence)
def __init__(
self,
left_overhang,
right_overhang,
left_addition="",
right_addition="",
enzyme="BsmBI",
extra_avoided_sites=(),
description="Golden Gate domesticator",
name="unnamed_domesticator",
cds_by_default=False,
constraints=(),
objectives=(),
):
self.enzyme = enzyme
self.left_overhang = left_overhang
left_overhang = sequence_to_biopython_record(left_overhang)
self.right_overhang = right_overhang
right_overhang = sequence_to_biopython_record(right_overhang)
for seq in [left_overhang, right_overhang]:
annotate_record(seq, label=str(seq.seq))
enzyme_seq = Restriction.__dict__[enzyme].site
enzyme_seq = sequence_to_biopython_record(enzyme_seq)
annotate_record(enzyme_seq, label=enzyme)
self.enzyme_seq = enzyme_seq
left_flank = self.enzyme_seq + "A" + left_overhang + left_addition
right_flank = (right_addition + right_overhang +
(self.enzyme_seq + "A").reverse_complement())
self.extra_avoided_sites = extra_avoided_sites
constraints = list(constraints) + [(lambda seq: AvoidPattern(
EnzymeSitePattern(enzyme),
location=Location(len(left_flank),
len(left_flank) + len(seq)),
)) for enz in ([enzyme] + list(extra_avoided_sites))]
PartDomesticator.__init__(
self,
left_flank=left_flank,
right_flank=right_flank,
constraints=constraints,
objectives=objectives,
description=description,
name=name,
cds_by_default=cds_by_default,
)
def make_restriction_part(part_length, left_overhang, right_overhang,
enzyme, forbidden_enzymes, assembly_enzyme='BsmBI'):
l_left = len(left_overhang)
l_right = len(right_overhang)
left_overhang_location = (0, l_left)
right_overhang_location = (l_left + part_length,
l_left + part_length + l_right)
center_location = (l_left, l_left + part_length)
core_sequence = (left_overhang + dc.random_dna_sequence(part_length)
+ right_overhang)
enforce_enzyme = dc.EnforcePatternOccurence(
enzyme=enzyme, location=center_location)
problem = dc.DnaOptimizationProblem(
sequence=core_sequence,
constraints=[
dc.AvoidChanges(left_overhang_location),
dc.AvoidChanges(right_overhang_location),
] + [enforce_enzyme] + [
dc.AvoidPattern(enzyme=enzyme_name)
for enzyme_name in forbidden_enzymes + [assembly_enzyme]
]
)
problem.resolve_constraints()
core_sequence = dc.sequence_to_biopython_record(problem.sequence)
for loc in [left_overhang_location, right_overhang_location]:
dc.annotate_record(core_sequence, loc, 'overhang')
site_location = enforce_enzyme.evaluate(problem).data['matches'][0]
dc.annotate_record(core_sequence, site_location.to_tuple(), enzyme)
assembly_site = Restriction.__dict__[assembly_enzyme].site
flank = dc.sequence_to_biopython_record(assembly_site + 'A')
dc.annotate_record(flank, label='flank')
return flank + core_sequence + flank.reverse_complement()
def test_parameterization():
def all_none(variables):
return all([c is None for c in variables])
problem1 = dc.DnaOptimizationProblem(
sequence=200 * "A",
constraints=[
dc.EnforceChanges(),
dc.EnforceChanges(minimum=20),
dc.EnforceChanges(minimum_percent=5),
],
objectives=[
dc.EnforceChanges(),
dc.EnforceChanges(amount=20),
dc.EnforceChanges(amount_percent=5),
],
)
record = dc.sequence_to_biopython_record(200 * "A")
dc.annotate_record(record, label="@change")
dc.annotate_record(record, label="@change(minimum=20)")
dc.annotate_record(record, label="@change(minimum=5%)")
dc.annotate_record(record, label="~change")
dc.annotate_record(record, label="~change(amount=20)")
dc.annotate_record(record, label="~change(5%)")
problem2 = dc.DnaOptimizationProblem.from_record(record)
for problem in [problem1, problem2]:
# CHECK CONSTRAINTS
c100 = problem.constraints[0]
assert c100.minimum == 200
assert c100.minimum_percent == 100
assert all_none([c100.amount, c100.amount_percent])
c20 = problem.constraints[1]
assert c20.minimum == 20
assert all_none([c20.minimum_percent, c20.amount, c20.amount_percent])
c5 = problem.constraints[2]
assert c5.minimum == 10
assert c5.minimum_percent == 5
assert all_none([c5.amount, c5.amount_percent])
# CHECK OBJECTIVES
o100 = problem.objectives[0]
assert o100.amount == 200
assert o100.amount_percent == 100
assert all_none([o100.minimum, o100.minimum_percent])
o20 = problem.objectives[1]
assert o20.amount == 20
assert all_none([o20.minimum_percent, o20.minimum, o20.amount_percent])
o5 = problem.objectives[2]
assert o5.amount == 10
assert o5.amount_percent == 5
assert all_none([o5.minimum, o5.minimum_percent])
def test_all_shorthands():
"""This test compiles all shorthands as a check that nothing is broken."""
numpy.random.seed(123)
sequence = random_dna_sequence(1000)
record = sequence_to_biopython_record(sequence)
annotate_record(record, (100, 900), label="@no(CATG)")
annotate_record(record, (100, 900), label="@gc(40-60%)")
annotate_record(record, (100, 900), label="@insert(AarI_site)")
annotate_record(record, (650, 752), label="@cds")
annotate_record(record, (100, 200), label="@keep")
annotate_record(record, (250, 273), label="@primer")
annotate_record(record, (250, 280), label="@change")
annotate_record(record, (943, 950), label="@sequence(AKGNTKT)")
annotate_record(record, (955, 958), label="@sequence(ATT|ATC|GGG)")
problem = DnaOptimizationProblem.from_record(record)
assert len(problem.constraints) == 13 # AllowPrimer counts for 4 specs.
assert not problem.all_constraints_pass()
problem.resolve_constraints()
assert problem.all_constraints_pass()
