def fetch_reads_refs_from_sampleset(ref, ws_url, srv_wiz_url):
"""
From the given object ref, return a list of all reads objects that are a part of that
object. E.g., if ref is a ReadsSet, return a list of all PairedEndLibrary or SingleEndLibrary
refs that are a member of that ReadsSet. This is returned as a list of dictionaries as follows:
{
"ref": reads object reference,
"condition": condition string associated with that reads object,
"name": reads object name (needed for saving an AlignmentSet)
}
The only one required is "ref", all other keys may or may not be present, based on the reads
object or object type in initial ref variable. E.g. a RNASeqSampleSet might have condition info
for each reads object, but a single PairedEndLibrary may not have that info.
If ref is already a Reads library, just returns a list with ref as a single element.
"""
obj_type = get_object_type(ref, ws_url)
refs = list()
if "KBaseSets.ReadsSet" in obj_type or "KBaseRNASeq.RNASeqSampleSet" in obj_type:
print("Looking up reads references in ReadsSet object")
set_client = SetAPI(srv_wiz_url)
reads_set = set_client.get_reads_set_v1({
"ref": ref,
"include_item_info": 0,
"include_set_item_ref_paths": 1
})
print("Got results from ReadsSet object")
pprint(reads_set)
ref_list = [r["ref_path"] for r in reads_set["data"]["items"]]
reads_names = get_object_names(ref_list, ws_url)
for reads in reads_set["data"]["items"]:
ref = reads["ref_path"]
refs.append({
"ref": ref,
"condition": reads["label"],
"name": reads_names[ref]
})
elif ("KBaseAssembly.SingleEndLibrary" in obj_type or
"KBaseFile.SingleEndLibrary" in obj_type or
"KBaseAssembly.PairedEndLibrary" in obj_type or
"KBaseFile.PairedEndLibrary" in obj_type):
refs.append({
"ref": ref,
"name": get_object_names([ref], ws_url)[ref]
})
else:
raise ValueError("Unable to fetch reads reference from object {} "
"which is a {}".format(ref, obj_type))
return refs
def test_run_hisat2_sampleset_ok(self):
res = self.get_impl().run_hisat2(self.get_context(), {
"ws_name": self.ws_name,
"sampleset_ref": self.single_end_sampleset,
"genome_ref": self.genome_ref,
"alignmentset_suffix": "_sampleset_alignments",
"alignment_suffix": "_sampleset_alignment",
"num_threads": 2,
"quality_score": "phred33",
"skip": 0,
"trim3": 0,
"trim5": 0,
"np": 1,
"min_intron_length": 20,
"max_intron_length": 500000,
"no_spliced_alignment": 0,
"transcriptome_mapping_only": 0,
"build_report": 1
})[0]
self.assertIsNotNone(res)
print("Done with HISAT2 run! {}".format(res))
self.assertIn("report_ref", res)
self.assertTrue(check_reference(res["report_ref"]))
self.assertIn("report_name", res)
self.assertIn("alignmentset_ref", res)
self.assertTrue(check_reference(res["alignmentset_ref"]))
self.assertTrue(get_object_names([res["alignmentset_ref"]], self.wsURL)[res["alignmentset_ref"]].endswith("_sampleset_alignments"))
self.assertIn("alignment_objs", res)
self.assertTrue(len(res["alignment_objs"].keys()) == 2)
for reads_ref in res["alignment_objs"]:
ref_from_refpath = reads_ref.split(';')[-1]
self.assertIn(ref_from_refpath, self.reads_refs)
self.assertTrue(res["alignment_objs"][reads_ref]["name"].endswith("_sampleset_alignment"))
self.assertTrue(check_reference(res["alignment_objs"][reads_ref]["ref"]))
def run_single(self, reads_ref, params):
"""
Performs a single run of HISAT2 against a single reads reference. The rest of the info
is taken from the params dict - see the spec for details.
"""
# 1. Get hisat2 index from genome.
# a. If it exists in cache, use that.
# b. Otherwise, build it
idx_prefix = self.build_index(params["genome_ref"])
# 2. Fetch the reads file and deal make sure input params are correct.
reads = fetch_reads_from_reference(reads_ref["ref"], self.callback_url)
# if the reads ref came from a different sample set, then we need to drop that
# reference inside the reads info object so it can be linked in the alignment
if reads_ref["ref"] != params["sampleset_ref"]:
reads["sampleset_ref"] = params["sampleset_ref"]
# make sure condition info carries over if we have it
if "condition" in reads_ref:
reads["condition"] = reads_ref["condition"]
elif "condition" in params:
reads["condition"] = params["condition"]
reads["name"] = reads_ref["name"]
output_file = "accepted_hits"
# 3. Finally all set, do the alignment and upload the output.
alignment_file = self.run_hisat2(idx_prefix,
reads,
params,
output_file=output_file)
alignment_name = reads["name"] + params["alignment_suffix"]
output_ref = self.upload_alignment(params, reads, alignment_name,
alignment_file)
alignment_set_ref = None
if is_set(params["sampleset_ref"], self.workspace_url):
# alignment_items, alignmentset_name, ws_name
set_name = get_object_names(
[params["sampleset_ref"]],
self.workspace_url)[params["sampleset_ref"]]
alignment_set_name = set_name + params["alignmentset_suffix"]
alignment_set_ref = self.upload_alignment_set(
[{
"ref": output_ref,
"label": reads["condition"]
}], alignment_set_name, params["ws_name"])
alignments = dict()
alignments[reads_ref["ref"]] = {
"ref": output_ref,
"name": alignment_name
}
os.remove(reads["file_fwd"])
if "file_rev" in reads:
os.remove(reads["file_rev"])
return (alignments, output_ref, alignment_set_ref)
def run_batch(self, reads_refs, params):
"""
Runs HISAT2 in batch mode.
reads_refs should be a list of dicts, where each looks like the following:
{
"ref": reads object reference,
"condition": condition for that ref (string)
}
"""
# build task list and send it to KBParallel
tasks = list()
set_name = get_object_names(
[params["sampleset_ref"]],
self.workspace_url)[params["sampleset_ref"]]
for idx, reads_ref in enumerate(reads_refs):
single_param = dict(params) # need a copy of the params
single_param["build_report"] = 0
single_param["sampleset_ref"] = reads_ref["ref"]
if "condition" in reads_ref:
single_param["condition"] = reads_ref["condition"]
else:
single_param["condition"] = "unspecified"
tasks.append({
"module_name": "kb_hisat2",
"function_name": "run_hisat2",
"version": self.my_version,
"parameters": single_param
})
# UNCOMMENT BELOW FOR LOCAL TESTING
batch_run_params = {
"tasks": tasks,
"runner": "parallel",
# "concurrent_local_tasks": 3,
# "concurrent_njsw_tasks": 0,
"max_retries": 2
}
parallel_runner = KBParallel(self.callback_url)
results = parallel_runner.run_batch(batch_run_params)["results"]
alignment_items = list()
alignments = dict()
for idx, result in enumerate(results):
# idx of the result is the same as the idx of the inputs AND reads_refs
if result["is_error"] != 0:
raise RuntimeError(
"Failed a parallel run of HISAT2! {}".format(
result["result_package"]["error"]))
reads_ref = tasks[idx]["parameters"]["sampleset_ref"]
alignment_items.append({
"ref":
result["result_package"]["result"][0]["alignment_objs"]
[reads_ref]["ref"],
"label":
reads_refs[idx].get("condition",
params.get("condition", "unspecified"))
})
alignments[reads_ref] = result["result_package"]["result"][0][
"alignment_objs"][reads_ref]
# build the final alignment set
output_ref = self.upload_alignment_set(
alignment_items, set_name + params["alignmentset_suffix"],
params["ws_name"])
return (alignments, output_ref)
