def test_sweep_tag_neighborhood():
lb = GraphLabels(20, 1e7, 4)
filename = utils.get_test_data('single-read.fq')
lb.graph.consume_fasta_and_tag(filename)
tags = lb.sweep_tag_neighborhood('CAGGCGCCCACCACCGTGCCCTCCAACCTGATGGT')
assert len(tags) == 1
assert tags.pop() == 173473779682
def test_sweep_tag_neighborhood():
lb = GraphLabels(20, 1e7, 4)
filename = utils.get_test_data('single-read.fq')
lb.graph.consume_fasta_and_tag(filename)
tags = lb.sweep_tag_neighborhood('CAGGCGCCCACCACCGTGCCCTCCAACCTGATGGT')
assert len(tags) == 1
assert list(tags) == [173473779682]
def test_label_tag_correctness_save_load():
lb_pre = GraphLabels(20, 1e7, 4)
filename = utils.get_test_data('test-labels.fa')
lb_pre.consume_fasta_and_tag_with_labels(filename)
# save labels to a file
savepath = utils.get_temp_filename('saved.labels')
lb_pre.save_labels_and_tags(savepath)
# trash the old GraphLabels
del lb_pre
# create new, load labels & tags
lb = GraphLabels(20, 1e7, 4)
lb.load_labels_and_tags(savepath)
# read A
labels = lb.sweep_label_neighborhood(
'ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCCTAGAGCTAGGCTAGGTGT')
print(
lb.sweep_tag_neighborhood(
'TTCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCTAGAGCTAGGCTAGGTGT'))
print(labels)
print(len('ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAG') - 19)
assert len(labels) == 2
assert 0 in labels
assert 1 in labels
# read B
labels = lb.sweep_label_neighborhood(
'GCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGCTCTGCCTAGAGCTAGGCTAGGTGTTGGGGATAG'
'ATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGA')
print(labels)
assert len(labels) == 3
assert 0 in labels
assert 1 in labels
assert 2 in labels
# read C
labels = lb.sweep_label_neighborhood(
'TGGGATAGATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGACCTAGAG'
'CTAGGCTAGGTGTTGGGGATAGATAGATAGATGAGTTGGGGATAGATAGATAGATGAGTGTAGATCCA'
'ACAACACATACA')
print(labels)
assert len(labels) == 2
assert 1 in labels
assert 2 in labels
# read D
labels = lb.sweep_label_neighborhood(
'TATATATATAGCTAGCTAGCTAACTAGCTAGCATCGATCGATCGATC')
print(labels)
assert len(labels) == 1
assert 3 in labels
def test_label_tag_correctness_save_load():
lb_pre = GraphLabels(20, 1e7, 4)
filename = utils.get_test_data('test-labels.fa')
lb_pre.consume_fasta_and_tag_with_labels(filename)
# save labels to a file
savepath = utils.get_temp_filename('saved.labels')
lb_pre.save_labels_and_tags(savepath)
# trash the old GraphLabels
del lb_pre
# create new, load labels & tags
lb = GraphLabels(20, 1e7, 4)
lb.load_labels_and_tags(savepath)
# read A
labels = lb.sweep_label_neighborhood(
'ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCCTAGAGCTAGGCTAGGTGT')
print(lb.sweep_tag_neighborhood(
'TTCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCTAGAGCTAGGCTAGGTGT'))
print(labels)
print(len('ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAG') - 19)
assert len(labels) == 2
assert 0 in labels
assert 1 in labels
# read B
labels = lb.sweep_label_neighborhood(
'GCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGCTCTGCCTAGAGCTAGGCTAGGTGTTGGGGATAG'
'ATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGA')
print(labels)
assert len(labels) == 3
assert 0 in labels
assert 1 in labels
assert 2 in labels
# read C
labels = lb.sweep_label_neighborhood(
'TGGGATAGATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGACCTAGAG'
'CTAGGCTAGGTGTTGGGGATAGATAGATAGATGAGTTGGGGATAGATAGATAGATGAGTGTAGATCCA'
'ACAACACATACA')
print(labels)
assert len(labels) == 2
assert 1 in labels
assert 2 in labels
# read D
labels = lb.sweep_label_neighborhood(
'TATATATATAGCTAGCTAGCTAACTAGCTAGCATCGATCGATCGATC')
print(labels)
assert len(labels) == 1
assert 3 in labels
def test_label_tag_correctness():
lb = GraphLabels(20, 1e7, 4)
filename = utils.get_test_data('test-labels.fa')
lb.consume_fasta_and_tag_with_labels(filename)
# read A
labels = lb.sweep_label_neighborhood(
'ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCCTAGAGCTAGGCTAGGTGT')
print(
lb.sweep_tag_neighborhood(
'TTCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCTAGAGCTAGGCTAGGTGT'))
print(labels)
print(len('ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAG') - 19)
assert len(labels) == 2
assert 0 in labels
assert 1 in labels
# read B
labels = lb.sweep_label_neighborhood(
'GCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGCTCTGCCTAGAGCTAGGCTAGGTGTTGGGGATAG'
'ATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGA')
print(labels)
assert len(labels) == 3
assert 0 in labels
assert 1 in labels
assert 2 in labels
# read C
labels = lb.sweep_label_neighborhood(
'TGGGATAGATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGACCTAGAG'
'CTAGGCTAGGTGTTGGGGATAGATAGATAGATGAGTTGGGGATAGATAGATAGATGAGTGTAGATCCA'
'ACAACACATACA')
print(labels)
assert len(labels) == 2
assert 1 in labels
assert 2 in labels
# read D
labels = lb.sweep_label_neighborhood(
'TATATATATAGCTAGCTAGCTAACTAGCTAGCATCGATCGATCGATC')
print(labels)
assert len(labels) == 1
assert 3 in labels
def test_label_tag_correctness():
lb = GraphLabels(20, 1e7, 4)
filename = utils.get_test_data('test-labels.fa')
lb.consume_fasta_and_tag_with_labels(filename)
# read A
labels = lb.sweep_label_neighborhood(
'ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCCTAGAGCTAGGCTAGGTGT')
print(lb.sweep_tag_neighborhood(
'TTCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGGCTCTGCCTAGAG'
'CTAGGCTAGGTGTGCTCTGCTAGAGCTAGGCTAGGTGT'))
print(labels)
print(len('ATCGTGTAAGCTATCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAG') - 19)
assert len(labels) == 2
assert 0 in labels
assert 1 in labels
# read B
labels = lb.sweep_label_neighborhood(
'GCGTAATCGTAAGCTCTGCCTAGAGCTAGGCTAGCTCTGCCTAGAGCTAGGCTAGGTGTTGGGGATAG'
'ATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGA')
print(labels)
assert len(labels) == 3
assert 0 in labels
assert 1 in labels
assert 2 in labels
# read C
labels = lb.sweep_label_neighborhood(
'TGGGATAGATAGATAGATGACCTAGAGCTAGGCTAGGTGTTGGGGATAGATAGATAGATGACCTAGAG'
'CTAGGCTAGGTGTTGGGGATAGATAGATAGATGAGTTGGGGATAGATAGATAGATGAGTGTAGATCCA'
'ACAACACATACA')
print(labels)
assert len(labels) == 2
assert 1 in labels
assert 2 in labels
# read D
labels = lb.sweep_label_neighborhood(
'TATATATATAGCTAGCTAGCTAACTAGCTAGCATCGATCGATCGATC')
print(labels)
assert len(labels) == 1
assert 3 in labels
def test_consume_fasta_and_tag_with_labels():
lb = GraphLabels(20, 1e7, 4)
read_1 = 'ACGTAACCGGTTAAACCCGGGTTTAAAACCCCGGGGTTTT'
filename = utils.get_test_data('test-transcript.fa')
total_reads, n_consumed = lb.consume_fasta_and_tag_with_labels(filename)
print("doing get")
assert lb.graph.get(read_1[:20])
assert total_reads == 3
print("doing n_labels")
print(lb.n_labels())
print("doing label dict")
print(lb.get_label_dict())
print("get tagset")
for tag in lb.graph.get_tagset():
print("forward hash")
print(tag, khmer.forward_hash(tag, 20))
for record in screed.open(filename):
print("Sweeping tags")
print(lb.sweep_tag_neighborhood(record.sequence, 40))
print("Sweeping labels...")
print(lb.sweep_label_neighborhood(record.sequence, 40))
assert lb.n_labels() == 3
def test_consume_fasta_and_tag_with_labels():
lb = GraphLabels(20, 1e7, 4)
read_1 = 'ACGTAACCGGTTAAACCCGGGTTTAAAACCCCGGGGTTTT'
filename = utils.get_test_data('test-transcript.fa')
total_reads, _ = lb.consume_fasta_and_tag_with_labels(filename)
print("doing get")
assert lb.graph.get(read_1[:20])
assert total_reads == 3
print("doing n_labels")
print(lb.n_labels())
print("doing all labels")
print(lb.get_all_labels())
print("get tagset")
for tag in lb.graph.get_tagset():
print("forward hash")
print(tag, khmer.forward_hash(tag, 20))
for record in screed.open(filename):
print("Sweeping tags")
print(lb.sweep_tag_neighborhood(record.sequence, 40))
print("Sweeping labels...")
print(lb.sweep_label_neighborhood(record.sequence, 40))
assert lb.n_labels() == 3
