def create_shotgun_process(user, norm_plate):
kappa = ReagentComposition(4)
stub = ReagentComposition(5)
shotgun_process = LibraryPrepShotgunProcess.create(
user, norm_plate, 'Test Shotgun Library %s' % datetime.now(), kappa,
stub, 4000, Plate(19), Plate(20))
shotgun_plate = shotgun_process.plates[0]
return shotgun_process, shotgun_plate
def create_shotgun_process(user, norm_plate):
kappa = ReagentComposition(4)
stub = ReagentComposition(5)
primer_a, primer_b = get_primer_plate(is_96=False)
shotgun_process = LibraryPrepShotgunProcess.create(
user, norm_plate, 'Test Shotgun Library %s' % datetime.now(), kappa,
stub, 4000, primer_a, primer_b)
shotgun_plate = shotgun_process.plates[0]
return shotgun_process, shotgun_plate
def post(self):
plate_name = self.get_argument('plate_name')
volume = self.get_argument('volume')
plate = self.get_argument('plate')
i5_plate = self.get_argument('i5_plate')
i7_plate = self.get_argument('i7_plate')
kappa_hyper_plus_kit = self.get_argument('kappa_hyper_plus_kit')
stub_lot = self.get_argument('stub_lot')
process = LibraryPrepShotgunProcess.create(
self.current_user, Plate(plate), plate_name,
ReagentComposition.from_external_id(kappa_hyper_plus_kit),
ReagentComposition.from_external_id(stub_lot), volume,
Plate(i5_plate), Plate(i7_plate))
self.write({'process': process.id})
def post(self):
user = self.current_user
plates_info = self.get_argument('plates_info')
volume = self.get_argument('volume')
kappa_hyper_plus_kit = self.get_argument('kappa_hyper_plus_kit')
stub_lot = self.get_argument('stub_lot')
processes = [[
pid,
LibraryPrepShotgunProcess.create(
user, Plate(pid), plate_name,
ReagentComposition.from_external_id(kappa_hyper_plus_kit),
ReagentComposition.from_external_id(stub_lot), volume,
Plate(i5p), Plate(i7p)).id
] for pid, plate_name, i5p, i7p in json_decode(plates_info)]
self.write({'processes': processes})