Example #1
0
    def _Run(self, parent, params, comm_title):

        input_files = m.ObtainImageFiles(params['Image Folder'])

        if len(input_files) == 0:
            print('No images in the Image Folder.')
            return False
        im = m.imread(input_files[0], cv2.IMREAD_UNCHANGED)
        root, ext_image = os.path.splitext(os.path.basename(input_files[0]))

        print('')
        print('Target file to check color type : ', input_files[0])
        print('Image dimensions                : ', im.shape)
        print('Image filetype                  : ', im.dtype)
        image_size_x = im.shape[1]
        image_size_y = im.shape[0]

        if (image_size_x <= 256 or image_size_y <= 256):
            print('Image size is too small.')
            return False

        # Generate tmpdir
        tmpdir_standardized = os.path.join(
            params['Output Segmentation Folder (Empty)'],
            "standardized" + str(threading.get_ident()).zfill(6)[-6:])
        if os.path.exists(tmpdir_standardized):
            shutil.rmtree(tmpdir_standardized)
        os.mkdir(tmpdir_standardized)
        #
        tmpdir_output = os.path.join(
            params['Output Segmentation Folder (Empty)'],
            "output" + str(threading.get_ident()).zfill(6)[-6:])
        if os.path.exists(tmpdir_output):
            shutil.rmtree(tmpdir_output)
        os.mkdir(tmpdir_output)

        ## Check image size
        max_image_size = params['Maximal unit image size']
        if max_image_size == '512':
            std_sizes = np.array([512])
        elif max_image_size == '1024':
            std_sizes = np.array([512, 1024])
        elif max_image_size == '2048':
            std_sizes = np.array([512, 1024, 2048])
        else:
            print('Internal error at Maximal unit image size.')
            return False

        max_std_size = np.max(std_sizes)
        if image_size_x > max_std_size:
            unit_image_size_x = max_std_size
            num_tiles_x = np.int(np.ceil(float(image_size_x) / max_std_size))
        else:
            unit_image_size_x = np.min(std_sizes[std_sizes >= image_size_x])
            num_tiles_x = 1

        if image_size_y > max_std_size:
            unit_image_size_y = max_std_size
            num_tiles_y = np.int(np.ceil(float(image_size_y) / max_std_size))
        else:
            unit_image_size_y = np.min(std_sizes[std_sizes >= image_size_y])
            num_tiles_y = 1
#
        converted_size_x = unit_image_size_x * num_tiles_x
        converted_size_y = unit_image_size_y * num_tiles_y
        fringe_size_x = converted_size_x - image_size_x
        fringe_size_y = converted_size_y - image_size_y

        #
        #
        output_files = []
        print('Image standardization: ')
        for input_file in input_files:
            im_col = m.imread(input_file)
            # im_col = self._ChangeIntoColor(im_col)

            filename = path.basename(input_file)
            print(filename + ' ')
            for ext in [
                    '.TIF', '.tif', '.TIFF', '.tiff', '.PNG', '.jpg', '.jpeg',
                    '.JPG', '.JPEG'
            ]:
                filename = filename.replace(ext, '.png')

            output_files.append(filename)

            # add fringe X
            im_fringe_x = cv2.flip(im_col, 1)  # flipcode > 0, left-right
            im_fringe_x = im_fringe_x[:, 0:fringe_size_x]
            converted_image = cv2.hconcat([im_col, im_fringe_x])
            # add fringe Y
            im_fringe_y = cv2.flip(converted_image,
                                   0)  # flipcode = 0, top-bottom
            im_fringe_y = im_fringe_y[0:fringe_size_y, :]
            converted_image = cv2.vconcat([converted_image, im_fringe_y])
            # Save
            if (num_tiles_x == 1) and (num_tiles_y == 1):
                converted_filename = os.path.join(tmpdir_standardized,
                                                  filename)
                m.imwrite(converted_filename, converted_image)
            else:
                for iy in range(num_tiles_y):
                    for ix in range(num_tiles_x):
                        y0 = iy * unit_image_size_y
                        y1 = y0 + unit_image_size_y
                        x0 = ix * unit_image_size_x
                        x1 = x0 + unit_image_size_x
                        current_tile = converted_image[y0:y1, x0:x1]
                        converted_filename = str(ix).zfill(3)[-3:] + '_' + str(
                            iy).zfill(3)[-3:] + '_' + filename
                        converted_filename = os.path.join(
                            tmpdir_standardized, converted_filename)
                        m.imwrite(converted_filename, current_tile)

        #Complete
        print('')
        print('Images were split and changed into RGB 8bit, and stored in ',
              tmpdir_standardized)
        print('')

        tmp = ['--mode'  , 'predict' , \
         '--save_freq' , '0'  , \
         '--input_dir' , tmpdir_standardized, \
   '--output_dir' , tmpdir_output, \
   '--checkpoint' , params['Model Folder'], \
            '--image_height', str(unit_image_size_y), \
            '--image_width' , str(unit_image_size_x)]

        comm = parent.u_info.exec_translate[:]
        comm.extend(tmp)

        print('')
        print('  '.join(comm))
        print('')
        print('Start inference.')
        print('')
        m.UnlockFolder(parent.u_info,
                       params['Output Segmentation Folder (Empty)']
                       )  # Only for shared folder/file
        s.run(comm)
        print('')
        print('Segmentation reconstruction: ')
        for output_file in output_files:
            ##
            if (num_tiles_x == 1) and (num_tiles_y == 1):
                ## Remove fringes
                filename = os.path.join(tmpdir_output, output_file)
                inferred_segmentation = m.imread(filename,
                                                 flags=cv2.IMREAD_GRAYSCALE,
                                                 dtype='uint8')
            else:
                ## Merge split images.
                inferred_segmentation = np.zeros(
                    (converted_size_y, converted_size_x), dtype='uint8')
                for iy in range(num_tiles_y):
                    for ix in range(num_tiles_x):
                        y0 = iy * unit_image_size_y
                        y1 = y0 + unit_image_size_y
                        x0 = ix * unit_image_size_x
                        x1 = x0 + unit_image_size_x
                        current_tile_filename = str(ix).zfill(
                            3)[-3:] + '_' + str(iy).zfill(
                                3)[-3:] + '_' + output_file
                        current_tile_filename = os.path.join(
                            tmpdir_output, current_tile_filename)
                        current_tile = m.imread(current_tile_filename,
                                                flags=cv2.IMREAD_GRAYSCALE,
                                                dtype='uint8')
                        inferred_segmentation[y0:y1,
                                              x0:x1] = current_tile[:, :]
            inferred_segmentation = inferred_segmentation[0:image_size_y,
                                                          0:image_size_x]

            print('inferred_segmentation: ', inferred_segmentation.shape,
                  inferred_segmentation.dtype)

            ## Save
            filename_base = os.path.splitext(os.path.basename(output_file))[0]
            filename_base = os.path.join(
                params['Output Segmentation Folder (Empty)'], filename_base)

            filetype = params['Output Filetype']

            if filetype == '8-bit gray scale PNG':
                filename = filename_base + '.png'
                m.save_png8(inferred_segmentation, filename)
            elif filetype == '8-bit gray scale TIFF (Uncompressed)':
                filename = filename_base + '.tif'
                m.save_tif8(inferred_segmentation, filename, compression=1)
            elif filetype == '8-bit gray scale TIFF (Compressed)':
                filename = filename_base + '.tif'
                m.save_tif8(inferred_segmentation, filename)
            else:
                print('Internel error: bad filetype.')
            print(filename)

        ##

# rm tmpdir
        if os.path.exists(tmpdir_standardized):
            shutil.rmtree(tmpdir_standardized)
        if os.path.exists(tmpdir_output):
            shutil.rmtree(tmpdir_output)

        parent.parent.ExecuteCloseFileFolder(
            params['Output Segmentation Folder (Empty)'])
        parent.parent.OpenFolder(params['Output Segmentation Folder (Empty)'])
        print('')
        print('Finish inference.')
        print('')
        return True
Example #2
0
    def _Run(self, parent, params, comm_title):
        ##
        ## Transform bitdepth of EM images and segmentation in the target directory.
        ## Translate.py only accepts unit24 (RGB color).
        ##
        img_files = glob.glob(os.path.join(params['Image Folder'], "*.jpg"))
        img_png = glob.glob(os.path.join(params['Image Folder'], "*.png"))
        img_tif = glob.glob(os.path.join(params['Image Folder'], "*.tif"))
        img_files.extend(img_png)
        img_files.extend(img_tif)
        img_files = sorted(img_files)
        if len(img_files) == 0:
            print('No image file.')
            return False

        im = m.imread(img_files[0], cv2.IMREAD_UNCHANGED)
        print('')
        print('Number of images : ', len(img_files))
        print('Image color type : ', img_files[0])
        print('Image dimensions : ', im.shape)
        print('Image filetype   : ', im.dtype)

        seg_files = glob.glob(
            os.path.join(params['Segmentation Folder'], "*.jpg"))
        seg_png = glob.glob(
            os.path.join(params['Segmentation Folder'], "*.png"))
        seg_tif = glob.glob(
            os.path.join(params['Segmentation Folder'], "*.tif"))
        seg_files.extend(seg_png)
        seg_files.extend(seg_tif)
        seg_files = sorted(seg_files)
        if len(seg_files) == 0:
            print('')
            print('No segmentation file.')
            print('Aborted.')
            return False

        sg = m.imread(seg_files[0], cv2.IMREAD_UNCHANGED)
        print('')
        print('Number of Segmentation images : ', len(seg_files))
        print('Segmentation image dimensions : ', sg.shape)
        print('Segmentation filetype         : ', sg.dtype)
        print('')

        if len(img_files) != len(seg_files):
            print(
                'The number of images is not equal to that of segmenation images.'
            )
            print('Aborted.')
            return False

# Generate tmpdir
        tmpdir = os.path.join(params['Model Folder (Empty)'], "paired" +
                              str(threading.get_ident()).zfill(6)[-6:])  #  )
        if os.path.exists(tmpdir):
            shutil.rmtree(tmpdir)
        os.mkdir(tmpdir)

        for img_file, seg_file in zip(img_files, seg_files):

            img = m.imread(img_file)
            seg = m.imread(seg_file)

            img = np.array(img, dtype=np.uint8)
            seg = np.array(seg, dtype=np.uint8)

            if len(img.shape) == 2:
                img = cv2.cvtColor(img, cv2.COLOR_GRAY2BGR)
            elif img.shape[2] == 4:
                img = cv2.cvtColor(img, cv2.COLOR_BGRA2BGR)
            elif img.shape[2] != 3:
                print('File is broken: ', img_file)
                print('Aborted.')
                return False

            if len(seg.shape) == 2:
                seg = cv2.cvtColor(seg, cv2.COLOR_GRAY2BGR)
            elif seg.shape[2] == 4:
                seg = cv2.cvtColor(seg, cv2.COLOR_BGRA2BGR)
            elif seg.shape[2] != 3:
                print('File is broken: ', seg_file)
                print('Aborted.')
                return False

            paired = cv2.hconcat([img, seg])

            tmpname = os.path.splitext(os.path.basename(img_file))[0]
            filename_paired = os.path.join(tmpdir, tmpname + '.png')
            m.imwrite(filename_paired, paired)

        print('Paired images (RGB 8bit) are stored in ', tmpdir)
        print('')

        #
        # Dialog to specify directory
        #

        aug = params['Augmentation']
        if aug == "fliplr, flipud, transpose":
            augmentation = ['--fliplr', '--flipud', '--transpose']
        elif aug == "fliplr, flipud":
            augmentation = ['--fliplr', '--flipud', '--no_transpose']
        elif aug == "fliplr":
            augmentation = ['--fliplr', '--no_flipud', '--no_transpose']
        elif aug == "flipud":
            augmentation = ['--no_fliplr', '--flipud', '--no_transpose']
        elif aug == "None":
            augmentation = ['--no_fliplr', '--no_flipud', '--no_transpose']
        else:
            print(
                "Internal error at Augumentation of PartDialogTrainingExecutor."
            )
            self._Cancel()
            return False
        #
        #   ' --model ' + params['Model'] + ' '
        #
# parent.u_info.exec_translate, \

        tmp = ['--batch_size'  , '4', \
            '--mode'   , 'train', \
   '--input_dir'  , tmpdir, \
   '--output_dir'  , params['Model Folder (Empty)'], \
            '--loss'   , params['Loss Function'], \
   '--network'  , params['Network'], \
         '--max_epochs'  , str( params['Maximal Epochs']  ),  \
         '--display_freq' , str( params['Display Frequency'] ),  \
         '--u_depth'  , str( params['U depth'] ),  \
         '--n_res_blocks' , str( params['N res blocks'] ), \
         '--n_highway_units', str( params['N highway units'] ), \
         '--n_dense_blocks' , str( params['N dense blocks'] ), \
         '--n_dense_layers' , str( params['N dense layers']) ]

        comm = parent.u_info.exec_translate[:]
        comm.extend(tmp)
        comm.extend(augmentation)

        print('')
        print('  '.join(comm))
        print('')
        print('Start training.')
        print('')
        m.UnlockFolder(parent.u_info, params['Model Folder (Empty)'])
        s.run(comm)
        # rm tmpdir
        if os.path.exists(tmpdir):
            shutil.rmtree(tmpdir)
        ## m.LockFolder(parent.u_info,  params['Model Folder (Empty)']) ## Tentatively commented out.
        parent.parent.ExecuteCloseFileFolder(params['Model Folder (Empty)'])
        parent.parent.OpenFolder(params['Model Folder (Empty)'])
        print('')
        print('Finish training.')
        print('')

        return True