def init_func_preproc_wf(bold_file):
"""
This workflow controls the functional preprocessing stages of *fMRIPrep*.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.tests import mock_config
from fmriprep import config
from fmriprep.workflows.bold.base import init_func_preproc_wf
with mock_config():
bold_file = config.execution.bids_dir / 'sub-01' / 'func' \
/ 'sub-01_task-mixedgamblestask_run-01_bold.nii.gz'
wf = init_func_preproc_wf(str(bold_file))
Parameters
----------
bold_file
BOLD series NIfTI file
Inputs
------
bold_file
BOLD series NIfTI file
t1w_preproc
Bias-corrected structural template image
t1w_mask
Mask of the skull-stripped template image
t1w_dseg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1w_asec
Segmentation of structural image, done with FreeSurfer.
t1w_aparc
Parcellation of structural image, done with FreeSurfer.
t1w_tpms
List of tissue probability maps in T1w space
template
List of templates to target
anat2std_xfm
List of transform files, collated with templates
std2anat_xfm
List of inverse transform files, collated with templates
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1w2fsnative_xfm
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
fsnative2t1w_xfm
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
Outputs
-------
bold_t1
BOLD series, resampled to T1w space
bold_mask_t1
BOLD series mask in T1w space
bold_std
BOLD series, resampled to template space
bold_mask_std
BOLD series mask in template space
confounds
TSV of confounds
surfaces
BOLD series, resampled to FreeSurfer surfaces
aroma_noise_ics
Noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
bold_cifti
BOLD CIFTI image
cifti_variant
combination of target spaces for `bold_cifti`
See Also
--------
* :py:func:`~niworkflows.func.util.init_bold_reference_wf`
* :py:func:`~fmriprep.workflows.bold.stc.init_bold_stc_wf`
* :py:func:`~fmriprep.workflows.bold.hmc.init_bold_hmc_wf`
* :py:func:`~fmriprep.workflows.bold.t2s.init_bold_t2s_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_t1_trans_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_bold_confounds_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_ica_aroma_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_std_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_preproc_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_surf_wf`
* :py:func:`~sdcflows.workflows.fmap.init_fmap_wf`
* :py:func:`~sdcflows.workflows.pepolar.init_pepolar_unwarp_wf`
* :py:func:`~sdcflows.workflows.phdiff.init_phdiff_wf`
* :py:func:`~sdcflows.workflows.syn.init_syn_sdc_wf`
* :py:func:`~sdcflows.workflows.unwarp.init_sdc_unwarp_wf`
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.func.util import init_bold_reference_wf
from niworkflows.interfaces.nibabel import ApplyMask
from niworkflows.interfaces.utility import KeySelect
from niworkflows.interfaces.utils import DictMerge
from sdcflows.workflows.base import init_sdc_estimate_wf, fieldmap_wrangler
ref_file = bold_file
mem_gb = {'filesize': 1, 'resampled': 1, 'largemem': 1}
bold_tlen = 10
multiecho = isinstance(bold_file, list)
# Have some options handy
layout = config.execution.layout
omp_nthreads = config.nipype.omp_nthreads
freesurfer = config.workflow.run_reconall
spaces = config.workflow.spaces
if multiecho:
tes = [layout.get_metadata(echo)['EchoTime'] for echo in bold_file]
ref_file = dict(zip(tes, bold_file))[min(tes)]
if os.path.isfile(ref_file):
bold_tlen, mem_gb = _create_mem_gb(ref_file)
wf_name = _get_wf_name(ref_file)
config.loggers.workflow.debug(
'Creating bold processing workflow for "%s" (%.2f GB / %d TRs). '
'Memory resampled/largemem=%.2f/%.2f GB.', ref_file,
mem_gb['filesize'], bold_tlen, mem_gb['resampled'], mem_gb['largemem'])
sbref_file = None
# Find associated sbref, if possible
entities = layout.parse_file_entities(ref_file)
entities['suffix'] = 'sbref'
entities['extension'] = ['nii', 'nii.gz'] # Overwrite extensions
files = layout.get(return_type='file', **entities)
refbase = os.path.basename(ref_file)
if 'sbref' in config.workflow.ignore:
config.loggers.workflow.info("Single-band reference files ignored.")
elif files and multiecho:
config.loggers.workflow.warning(
"Single-band reference found, but not supported in "
"multi-echo workflows at this time. Ignoring.")
elif files:
sbref_file = files[0]
sbbase = os.path.basename(sbref_file)
if len(files) > 1:
config.loggers.workflow.warning(
"Multiple single-band reference files found for {}; using "
"{}".format(refbase, sbbase))
else:
config.loggers.workflow.info(
"Using single-band reference file %s.", sbbase)
else:
config.loggers.workflow.info("No single-band-reference found for %s.",
refbase)
metadata = layout.get_metadata(ref_file)
# Find fieldmaps. Options: (phase1|phase2|phasediff|epi|fieldmap|syn)
fmaps = None
if 'fieldmaps' not in config.workflow.ignore:
fmaps = fieldmap_wrangler(layout,
ref_file,
use_syn=config.workflow.use_syn,
force_syn=config.workflow.force_syn)
elif config.workflow.use_syn or config.workflow.force_syn:
# If fieldmaps are not enabled, activate SyN-SDC in unforced (False) mode
fmaps = {'syn': False}
# Short circuits: (True and True and (False or 'TooShort')) == 'TooShort'
run_stc = (bool(metadata.get("SliceTiming"))
and 'slicetiming' not in config.workflow.ignore
and (_get_series_len(ref_file) > 4 or "TooShort"))
# Check if MEEPI for T2* coregistration target
if config.workflow.t2s_coreg and not multiecho:
config.loggers.workflow.warning(
"No multiecho BOLD images found for T2* coregistration. "
"Using standard EPI-T1 coregistration.")
config.workflow.t2s_coreg = False
# By default, force-bbr for t2s_coreg unless user specifies otherwise
if config.workflow.t2s_coreg and config.workflow.use_bbr is None:
config.workflow.use_bbr = True
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__postdesc__ = """\
All resamplings can be performed with *a single interpolation
step* by composing all the pertinent transformations (i.e. head-motion
transform matrices, susceptibility distortion correction when available,
and co-registrations to anatomical and output spaces).
Gridded (volumetric) resamplings were performed using `antsApplyTransforms` (ANTs),
configured with Lanczos interpolation to minimize the smoothing
effects of other kernels [@lanczos].
Non-gridded (surface) resamplings were performed using `mri_vol2surf`
(FreeSurfer).
"""
inputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_file', 'subjects_dir', 'subject_id', 't1w_preproc', 't1w_mask',
't1w_dseg', 't1w_tpms', 't1w_aseg', 't1w_aparc', 'anat2std_xfm',
'std2anat_xfm', 'template', 't1w2fsnative_xfm', 'fsnative2t1w_xfm'
]),
name='inputnode')
inputnode.inputs.bold_file = bold_file
if sbref_file is not None:
from niworkflows.interfaces.images import ValidateImage
val_sbref = pe.Node(ValidateImage(in_file=sbref_file),
name='val_sbref')
outputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_t1', 'bold_t1_ref', 'bold_mask_t1', 'bold_aseg_t1',
'bold_aparc_t1', 'bold_std', 'bold_std_ref', 'bold_mask_std',
'bold_aseg_std', 'bold_aparc_std', 'bold_native', 'bold_cifti',
'cifti_variant', 'cifti_metadata', 'cifti_density', 'surfaces',
'confounds', 'aroma_noise_ics', 'melodic_mix', 'nonaggr_denoised_file',
'confounds_metadata'
]),
name='outputnode')
# Generate a brain-masked conversion of the t1w
t1w_brain = pe.Node(ApplyMask(), name='t1w_brain')
# BOLD buffer: an identity used as a pointer to either the original BOLD
# or the STC'ed one for further use.
boldbuffer = pe.Node(niu.IdentityInterface(fields=['bold_file']),
name='boldbuffer')
summary = pe.Node(FunctionalSummary(
slice_timing=run_stc,
registration=('FSL', 'FreeSurfer')[freesurfer],
registration_dof=config.workflow.bold2t1w_dof,
registration_init=config.workflow.bold2t1w_init,
pe_direction=metadata.get("PhaseEncodingDirection"),
tr=metadata.get("RepetitionTime")),
name='summary',
mem_gb=config.DEFAULT_MEMORY_MIN_GB,
run_without_submitting=True)
summary.inputs.dummy_scans = config.workflow.dummy_scans
func_derivatives_wf = init_func_derivatives_wf(
bids_root=layout.root,
cifti_output=config.workflow.cifti_output,
freesurfer=freesurfer,
metadata=metadata,
output_dir=str(config.execution.output_dir),
spaces=spaces,
use_aroma=config.workflow.use_aroma,
)
workflow.connect([
(outputnode, func_derivatives_wf, [
('bold_t1', 'inputnode.bold_t1'),
('bold_t1_ref', 'inputnode.bold_t1_ref'),
('bold_aseg_t1', 'inputnode.bold_aseg_t1'),
('bold_aparc_t1', 'inputnode.bold_aparc_t1'),
('bold_mask_t1', 'inputnode.bold_mask_t1'),
('bold_native', 'inputnode.bold_native'),
('confounds', 'inputnode.confounds'),
('surfaces', 'inputnode.surf_files'),
('aroma_noise_ics', 'inputnode.aroma_noise_ics'),
('melodic_mix', 'inputnode.melodic_mix'),
('nonaggr_denoised_file', 'inputnode.nonaggr_denoised_file'),
('bold_cifti', 'inputnode.bold_cifti'),
('cifti_variant', 'inputnode.cifti_variant'),
('cifti_metadata', 'inputnode.cifti_metadata'),
('cifti_density', 'inputnode.cifti_density'),
('confounds_metadata', 'inputnode.confounds_metadata'),
]),
])
# Generate a tentative boldref
bold_reference_wf = init_bold_reference_wf(omp_nthreads=omp_nthreads)
bold_reference_wf.inputs.inputnode.dummy_scans = config.workflow.dummy_scans
if sbref_file is not None:
workflow.connect([
(val_sbref, bold_reference_wf, [('out_file',
'inputnode.sbref_file')]),
])
# Top-level BOLD splitter
bold_split = pe.Node(FSLSplit(dimension='t'),
name='bold_split',
mem_gb=mem_gb['filesize'] * 3)
# HMC on the BOLD
bold_hmc_wf = init_bold_hmc_wf(name='bold_hmc_wf',
mem_gb=mem_gb['filesize'],
omp_nthreads=omp_nthreads)
# calculate BOLD registration to T1w
bold_reg_wf = init_bold_reg_wf(name='bold_reg_wf',
freesurfer=freesurfer,
use_bbr=config.workflow.use_bbr,
bold2t1w_dof=config.workflow.bold2t1w_dof,
bold2t1w_init=config.workflow.bold2t1w_init,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# apply BOLD registration to T1w
bold_t1_trans_wf = init_bold_t1_trans_wf(name='bold_t1_trans_wf',
freesurfer=freesurfer,
use_fieldwarp=bool(fmaps),
multiecho=multiecho,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# get confounds
bold_confounds_wf = init_bold_confs_wf(
mem_gb=mem_gb['largemem'],
metadata=metadata,
regressors_all_comps=config.workflow.regressors_all_comps,
regressors_fd_th=config.workflow.regressors_fd_th,
regressors_dvars_th=config.workflow.regressors_dvars_th,
name='bold_confounds_wf')
bold_confounds_wf.get_node('inputnode').inputs.t1_transform_flags = [False]
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_bold_trans_wf = init_bold_preproc_trans_wf(
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=not config.execution.low_mem,
use_fieldwarp=bool(fmaps),
name='bold_bold_trans_wf')
bold_bold_trans_wf.inputs.inputnode.name_source = ref_file
# SLICE-TIME CORRECTION (or bypass) #############################################
if run_stc is True: # bool('TooShort') == True, so check True explicitly
bold_stc_wf = init_bold_stc_wf(name='bold_stc_wf', metadata=metadata)
workflow.connect([
(bold_reference_wf, bold_stc_wf, [('outputnode.skip_vols',
'inputnode.skip_vols')]),
(bold_stc_wf, boldbuffer, [('outputnode.stc_file', 'bold_file')]),
])
if not multiecho:
workflow.connect([(bold_reference_wf, bold_stc_wf, [
('outputnode.bold_file', 'inputnode.bold_file')
])])
else: # for meepi, iterate through stc_wf for all workflows
meepi_echos = boldbuffer.clone(name='meepi_echos')
meepi_echos.iterables = ('bold_file', bold_file)
workflow.connect([(meepi_echos, bold_stc_wf,
[('bold_file', 'inputnode.bold_file')])])
elif not multiecho: # STC is too short or False
# bypass STC from original BOLD to the splitter through boldbuffer
workflow.connect([(bold_reference_wf, boldbuffer,
[('outputnode.bold_file', 'bold_file')])])
else:
# for meepi, iterate over all meepi echos to boldbuffer
boldbuffer.iterables = ('bold_file', bold_file)
# SDC (SUSCEPTIBILITY DISTORTION CORRECTION) or bypass ##########################
bold_sdc_wf = init_sdc_estimate_wf(fmaps,
metadata,
omp_nthreads=omp_nthreads,
debug=config.execution.debug)
# MULTI-ECHO EPI DATA #############################################
if multiecho:
from niworkflows.func.util import init_skullstrip_bold_wf
skullstrip_bold_wf = init_skullstrip_bold_wf(name='skullstrip_bold_wf')
inputnode.inputs.bold_file = ref_file # Replace reference w first echo
join_echos = pe.JoinNode(
niu.IdentityInterface(fields=['bold_files']),
joinsource=('meepi_echos' if run_stc is True else 'boldbuffer'),
joinfield=['bold_files'],
name='join_echos')
# create optimal combination, adaptive T2* map
bold_t2s_wf = init_bold_t2s_wf(echo_times=tes,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
t2s_coreg=config.workflow.t2s_coreg,
name='bold_t2smap_wf')
workflow.connect([
(skullstrip_bold_wf, join_echos,
[('outputnode.skull_stripped_file', 'bold_files')]),
(join_echos, bold_t2s_wf, [('bold_files', 'inputnode.bold_file')]),
])
# MAIN WORKFLOW STRUCTURE #######################################################
workflow.connect([
(inputnode, t1w_brain, [('t1w_preproc', 'in_file'),
('t1w_mask', 'in_mask')]),
# Generate early reference
(inputnode, bold_reference_wf, [('bold_file', 'inputnode.bold_file')]),
# BOLD buffer has slice-time corrected if it was run, original otherwise
(boldbuffer, bold_split, [('bold_file', 'in_file')]),
# HMC
(bold_reference_wf, bold_hmc_wf,
[('outputnode.raw_ref_image', 'inputnode.raw_ref_image'),
('outputnode.bold_file', 'inputnode.bold_file')]),
(bold_reference_wf, summary, [('outputnode.algo_dummy_scans',
'algo_dummy_scans')]),
# EPI-T1 registration workflow
(
inputnode,
bold_reg_wf,
[
('t1w_dseg', 'inputnode.t1w_dseg'),
# Undefined if --fs-no-reconall, but this is safe
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('fsnative2t1w_xfm', 'inputnode.fsnative2t1w_xfm')
]),
(t1w_brain, bold_reg_wf, [('out_file', 'inputnode.t1w_brain')]),
(inputnode, bold_t1_trans_wf, [('bold_file', 'inputnode.name_source'),
('t1w_mask', 'inputnode.t1w_mask'),
('t1w_aseg', 'inputnode.t1w_aseg'),
('t1w_aparc', 'inputnode.t1w_aparc')]),
(t1w_brain, bold_t1_trans_wf, [('out_file', 'inputnode.t1w_brain')]),
# unused if multiecho, but this is safe
(bold_hmc_wf, bold_t1_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_t1_trans_wf, [('outputnode.itk_bold_to_t1',
'inputnode.itk_bold_to_t1')]),
(bold_t1_trans_wf, outputnode,
[('outputnode.bold_t1', 'bold_t1'),
('outputnode.bold_t1_ref', 'bold_t1_ref'),
('outputnode.bold_aseg_t1', 'bold_aseg_t1'),
('outputnode.bold_aparc_t1', 'bold_aparc_t1')]),
(bold_reg_wf, summary, [('outputnode.fallback', 'fallback')]),
# SDC (or pass-through workflow)
(t1w_brain, bold_sdc_wf, [('out_file', 'inputnode.t1w_brain')]),
(bold_reference_wf, bold_sdc_wf,
[('outputnode.ref_image', 'inputnode.epi_file'),
('outputnode.ref_image_brain', 'inputnode.epi_brain'),
('outputnode.bold_mask', 'inputnode.epi_mask')]),
(bold_sdc_wf, bold_t1_trans_wf, [('outputnode.out_warp',
'inputnode.fieldwarp')]),
(bold_sdc_wf, bold_bold_trans_wf,
[('outputnode.out_warp', 'inputnode.fieldwarp'),
('outputnode.epi_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, summary, [('outputnode.method', 'distortion_correction')
]),
# Connect bold_confounds_wf
(inputnode, bold_confounds_wf, [('t1w_tpms', 'inputnode.t1w_tpms'),
('t1w_mask', 'inputnode.t1w_mask')]),
(bold_hmc_wf, bold_confounds_wf, [('outputnode.movpar_file',
'inputnode.movpar_file')]),
(bold_reg_wf, bold_confounds_wf, [('outputnode.itk_t1_to_bold',
'inputnode.t1_bold_xform')]),
(bold_reference_wf, bold_confounds_wf, [('outputnode.skip_vols',
'inputnode.skip_vols')]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_metadata', 'confounds_metadata'),
]),
# Connect bold_bold_trans_wf
(bold_split, bold_bold_trans_wf, [('out_files', 'inputnode.bold_file')]
),
(bold_hmc_wf, bold_bold_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
# Summary
(outputnode, summary, [('confounds', 'confounds_file')]),
])
if not config.workflow.t2s_coreg:
workflow.connect([
(bold_sdc_wf, bold_reg_wf, [('outputnode.epi_brain',
'inputnode.ref_bold_brain')]),
(bold_sdc_wf, bold_t1_trans_wf,
[('outputnode.epi_brain', 'inputnode.ref_bold_brain'),
('outputnode.epi_mask', 'inputnode.ref_bold_mask')]),
])
else:
workflow.connect([
# For t2s_coreg, replace EPI-to-T1w registration inputs
(bold_t2s_wf, bold_reg_wf, [('outputnode.bold_ref_brain',
'inputnode.ref_bold_brain')]),
(bold_t2s_wf, bold_t1_trans_wf,
[('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain'),
('outputnode.bold_mask', 'inputnode.ref_bold_mask')]),
])
# for standard EPI data, pass along correct file
if not multiecho:
workflow.connect([
(inputnode, func_derivatives_wf, [('bold_file',
'inputnode.source_file')]),
(bold_bold_trans_wf, bold_confounds_wf,
[('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_split, bold_t1_trans_wf, [('out_files',
'inputnode.bold_split')]),
])
else: # for meepi, create and use optimal combination
workflow.connect([
# update name source for optimal combination
(inputnode, func_derivatives_wf,
[(('bold_file', combine_meepi_source), 'inputnode.source_file')]),
(bold_bold_trans_wf, skullstrip_bold_wf, [('outputnode.bold',
'inputnode.in_file')]),
(bold_t2s_wf, bold_confounds_wf,
[('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_t2s_wf, bold_t1_trans_wf, [('outputnode.bold',
'inputnode.bold_split')]),
])
if fmaps:
from sdcflows.workflows.outputs import init_sdc_unwarp_report_wf
# Report on BOLD correction
fmap_unwarp_report_wf = init_sdc_unwarp_report_wf()
workflow.connect([
(inputnode, fmap_unwarp_report_wf, [('t1w_dseg',
'inputnode.in_seg')]),
(bold_reference_wf, fmap_unwarp_report_wf,
[('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, fmap_unwarp_report_wf, [('outputnode.itk_t1_to_bold',
'inputnode.in_xfm')]),
(bold_sdc_wf, fmap_unwarp_report_wf, [('outputnode.epi_corrected',
'inputnode.in_post')]),
])
# Overwrite ``out_path_base`` of unwarping DataSinks
for node in fmap_unwarp_report_wf.list_node_names():
if node.split('.')[-1].startswith('ds_'):
fmap_unwarp_report_wf.get_node(
node).interface.out_path_base = 'fmriprep'
for node in bold_sdc_wf.list_node_names():
if node.split('.')[-1].startswith('ds_'):
bold_sdc_wf.get_node(node).interface.out_path_base = 'fmriprep'
if 'syn' in fmaps:
sdc_select_std = pe.Node(KeySelect(fields=['std2anat_xfm']),
name='sdc_select_std',
run_without_submitting=True)
sdc_select_std.inputs.key = 'MNI152NLin2009cAsym'
workflow.connect([
(inputnode, sdc_select_std, [('std2anat_xfm', 'std2anat_xfm'),
('template', 'keys')]),
(sdc_select_std, bold_sdc_wf, [('std2anat_xfm',
'inputnode.std2anat_xfm')]),
])
if fmaps.get('syn') is True: # SyN forced
syn_unwarp_report_wf = init_sdc_unwarp_report_wf(
name='syn_unwarp_report_wf', forcedsyn=True)
workflow.connect([
(inputnode, syn_unwarp_report_wf, [('t1w_dseg',
'inputnode.in_seg')]),
(bold_reference_wf, syn_unwarp_report_wf,
[('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, syn_unwarp_report_wf,
[('outputnode.itk_t1_to_bold', 'inputnode.in_xfm')]),
(bold_sdc_wf, syn_unwarp_report_wf, [('outputnode.syn_ref',
'inputnode.in_post')]),
])
# Overwrite ``out_path_base`` of unwarping DataSinks
for node in syn_unwarp_report_wf.list_node_names():
if node.split('.')[-1].startswith('ds_'):
syn_unwarp_report_wf.get_node(
node).interface.out_path_base = 'fmriprep'
# Map final BOLD mask into T1w space (if required)
nonstd_spaces = set(spaces.get_nonstandard())
if nonstd_spaces.intersection(('T1w', 'anat')):
from niworkflows.interfaces.fixes import (FixHeaderApplyTransforms as
ApplyTransforms)
boldmask_to_t1w = pe.Node(ApplyTransforms(interpolation='MultiLabel',
float=True),
name='boldmask_to_t1w',
mem_gb=0.1)
workflow.connect([
(bold_reg_wf, boldmask_to_t1w, [('outputnode.itk_bold_to_t1',
'transforms')]),
(bold_t1_trans_wf, boldmask_to_t1w, [('outputnode.bold_mask_t1',
'reference_image')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
boldmask_to_t1w, [('outputnode.bold_mask', 'input_image')]),
(boldmask_to_t1w, outputnode, [('output_image', 'bold_mask_t1')]),
])
if nonstd_spaces.intersection(('func', 'run', 'bold', 'boldref', 'sbref')):
workflow.connect([
(bold_bold_trans_wf, outputnode, [('outputnode.bold',
'bold_native')]),
(bold_bold_trans_wf, func_derivatives_wf,
[('outputnode.bold_ref', 'inputnode.bold_native_ref'),
('outputnode.bold_mask', 'inputnode.bold_mask_native')]),
])
if spaces.get_spaces(nonstandard=False, dim=(3, )):
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_std_trans_wf = init_bold_std_trans_wf(
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
spaces=spaces,
name='bold_std_trans_wf',
use_compression=not config.execution.low_mem,
use_fieldwarp=bool(fmaps),
)
workflow.connect([
(inputnode, bold_std_trans_wf,
[('template', 'inputnode.templates'),
('anat2std_xfm', 'inputnode.anat2std_xfm'),
('bold_file', 'inputnode.name_source'),
('t1w_aseg', 'inputnode.bold_aseg'),
('t1w_aparc', 'inputnode.bold_aparc')]),
(bold_hmc_wf, bold_std_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_std_trans_wf, [('outputnode.itk_bold_to_t1',
'inputnode.itk_bold_to_t1')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
bold_std_trans_wf, [('outputnode.bold_mask',
'inputnode.bold_mask')]),
(bold_sdc_wf, bold_std_trans_wf, [('outputnode.out_warp',
'inputnode.fieldwarp')]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_std', 'bold_std'),
('outputnode.bold_std_ref', 'bold_std_ref'),
('outputnode.bold_mask_std', 'bold_mask_std')]),
])
if freesurfer:
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('outputnode.bold_aseg_std', 'inputnode.bold_aseg_std'),
('outputnode.bold_aparc_std', 'inputnode.bold_aparc_std'),
]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_aseg_std', 'bold_aseg_std'),
('outputnode.bold_aparc_std', 'bold_aparc_std')]),
])
if not multiecho:
workflow.connect([(bold_split, bold_std_trans_wf,
[('out_files', 'inputnode.bold_split')])])
else:
split_opt_comb = bold_split.clone(name='split_opt_comb')
workflow.connect([(bold_t2s_wf, split_opt_comb,
[('outputnode.bold', 'in_file')]),
(split_opt_comb, bold_std_trans_wf,
[('out_files', 'inputnode.bold_split')])])
# func_derivatives_wf internally parametrizes over snapshotted spaces.
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('outputnode.template', 'inputnode.template'),
('outputnode.spatial_reference',
'inputnode.spatial_reference'),
('outputnode.bold_std_ref', 'inputnode.bold_std_ref'),
('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
]),
])
if config.workflow.use_aroma: # ICA-AROMA workflow
from .confounds import init_ica_aroma_wf
ica_aroma_wf = init_ica_aroma_wf(
mem_gb=mem_gb['resampled'],
metadata=metadata,
omp_nthreads=omp_nthreads,
use_fieldwarp=bool(fmaps),
err_on_aroma_warn=config.workflow.aroma_err_on_warn,
aroma_melodic_dim=config.workflow.aroma_melodic_dim,
name='ica_aroma_wf')
join = pe.Node(niu.Function(output_names=["out_file"],
function=_to_join),
name='aroma_confounds')
mrg_conf_metadata = pe.Node(niu.Merge(2),
name='merge_confound_metadata',
run_without_submitting=True)
mrg_conf_metadata2 = pe.Node(DictMerge(),
name='merge_confound_metadata2',
run_without_submitting=True)
workflow.disconnect([
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_metadata', 'confounds_metadata'),
]),
])
workflow.connect([
(inputnode, ica_aroma_wf, [('bold_file',
'inputnode.name_source')]),
(bold_hmc_wf, ica_aroma_wf, [('outputnode.movpar_file',
'inputnode.movpar_file')]),
(bold_reference_wf, ica_aroma_wf, [('outputnode.skip_vols',
'inputnode.skip_vols')]),
(bold_confounds_wf, join, [('outputnode.confounds_file',
'in_file')]),
(bold_confounds_wf, mrg_conf_metadata,
[('outputnode.confounds_metadata', 'in1')]),
(ica_aroma_wf, join, [('outputnode.aroma_confounds',
'join_file')]),
(ica_aroma_wf, mrg_conf_metadata,
[('outputnode.aroma_metadata', 'in2')]),
(mrg_conf_metadata, mrg_conf_metadata2, [('out', 'in_dicts')]),
(ica_aroma_wf, outputnode,
[('outputnode.aroma_noise_ics', 'aroma_noise_ics'),
('outputnode.melodic_mix', 'melodic_mix'),
('outputnode.nonaggr_denoised_file', 'nonaggr_denoised_file')
]),
(join, outputnode, [('out_file', 'confounds')]),
(mrg_conf_metadata2, outputnode, [('out_dict',
'confounds_metadata')]),
(bold_std_trans_wf, ica_aroma_wf,
[('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
('outputnode.spatial_reference',
'inputnode.spatial_reference')]),
])
# SURFACES ##################################################################################
# Freesurfer
freesurfer_spaces = spaces.get_fs_spaces()
if freesurfer and freesurfer_spaces:
config.loggers.workflow.debug(
'Creating BOLD surface-sampling workflow.')
bold_surf_wf = init_bold_surf_wf(
mem_gb=mem_gb['resampled'],
surface_spaces=freesurfer_spaces,
medial_surface_nan=config.workflow.medial_surface_nan,
name='bold_surf_wf')
workflow.connect([
(inputnode, bold_surf_wf,
[('t1w_preproc', 'inputnode.t1w_preproc'),
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1w2fsnative_xfm', 'inputnode.t1w2fsnative_xfm')]),
(bold_t1_trans_wf, bold_surf_wf, [('outputnode.bold_t1',
'inputnode.source_file')]),
(bold_surf_wf, outputnode, [('outputnode.surfaces', 'surfaces')]),
(bold_surf_wf, func_derivatives_wf, [('outputnode.target',
'inputnode.surf_refs')]),
])
# CIFTI output
if config.workflow.cifti_output:
from .resampling import init_bold_grayords_wf
bold_grayords_wf = init_bold_grayords_wf(
grayord_density=config.workflow.cifti_output,
mem_gb=mem_gb['resampled'],
repetition_time=metadata['RepetitionTime'])
workflow.connect([
(inputnode, bold_grayords_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bold_std_trans_wf, bold_grayords_wf,
[('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.spatial_reference',
'inputnode.spatial_reference')]),
(bold_surf_wf, bold_grayords_wf, [
('outputnode.surfaces', 'inputnode.surf_files'),
('outputnode.target', 'inputnode.surf_refs'),
]),
(bold_grayords_wf, outputnode,
[('outputnode.cifti_bold', 'bold_cifti'),
('outputnode.cifti_variant', 'cifti_variant'),
('outputnode.cifti_metadata', 'cifti_metadata'),
('outputnode.cifti_density', 'cifti_density')]),
])
if spaces.get_spaces(nonstandard=False, dim=(3, )):
carpetplot_wf = init_carpetplot_wf(
mem_gb=mem_gb['resampled'],
metadata=metadata,
cifti_output=config.workflow.cifti_output,
name='carpetplot_wf')
if config.workflow.cifti_output:
workflow.connect(bold_grayords_wf, 'outputnode.cifti_bold',
carpetplot_wf, 'inputnode.cifti_bold')
else:
# Xform to 'MNI152NLin2009cAsym' is always computed.
carpetplot_select_std = pe.Node(KeySelect(
fields=['std2anat_xfm'], key='MNI152NLin2009cAsym'),
name='carpetplot_select_std',
run_without_submitting=True)
workflow.connect([
(inputnode, carpetplot_select_std, [('std2anat_xfm',
'std2anat_xfm'),
('template', 'keys')]),
(carpetplot_select_std, carpetplot_wf,
[('std2anat_xfm', 'inputnode.std2anat_xfm')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
carpetplot_wf, [('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask',
'inputnode.bold_mask')]),
(bold_reg_wf, carpetplot_wf, [('outputnode.itk_t1_to_bold',
'inputnode.t1_bold_xform')]),
])
workflow.connect([(bold_confounds_wf, carpetplot_wf, [
('outputnode.confounds_file', 'inputnode.confounds_file')
])])
# REPORTING ############################################################
reportlets_dir = str(config.execution.work_dir / 'reportlets')
ds_report_summary = pe.Node(DerivativesDataSink(desc='summary',
keep_dtype=True),
name='ds_report_summary',
run_without_submitting=True,
mem_gb=config.DEFAULT_MEMORY_MIN_GB)
ds_report_validation = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='validation', keep_dtype=True),
name='ds_report_validation',
run_without_submitting=True,
mem_gb=config.DEFAULT_MEMORY_MIN_GB)
workflow.connect([
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bold_reference_wf, ds_report_validation,
[('outputnode.validation_report', 'in_file')]),
])
# Fill-in datasinks of reportlets seen so far
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_report'):
workflow.get_node(node).inputs.base_directory = reportlets_dir
workflow.get_node(node).inputs.source_file = ref_file
return workflow
def init_single_subject_wf(
debug,
freesurfer,
fast_track,
hires,
layout,
longitudinal,
low_mem,
name,
omp_nthreads,
output_dir,
skull_strip_fixed_seed,
skull_strip_mode,
skull_strip_template,
spaces,
subject_id,
bids_filters,
):
"""
Create a single subject workflow.
This workflow organizes the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from collections import namedtuple
from niworkflows.utils.spaces import SpatialReferences, Reference
from smriprep.workflows.base import init_single_subject_wf
BIDSLayout = namedtuple('BIDSLayout', ['root'])
wf = init_single_subject_wf(
debug=False,
freesurfer=True,
fast_track=False,
hires=True,
layout=BIDSLayout('.'),
longitudinal=False,
low_mem=False,
name='single_subject_wf',
omp_nthreads=1,
output_dir='.',
skull_strip_fixed_seed=False,
skull_strip_mode='force',
skull_strip_template=Reference('OASIS30ANTs'),
spaces=SpatialReferences(spaces=['MNI152NLin2009cAsym', 'fsaverage5']),
subject_id='test',
bids_filters=None,
)
Parameters
----------
debug : :obj:`bool`
Enable debugging outputs
freesurfer : :obj:`bool`
Enable FreeSurfer surface reconstruction (may increase runtime)
fast_track : :obj:`bool`
If ``True``, attempt to collect previously run derivatives.
hires : :obj:`bool`
Enable sub-millimeter preprocessing in FreeSurfer
layout : BIDSLayout object
BIDS dataset layout
longitudinal : :obj:`bool`
Treat multiple sessions as longitudinal (may increase runtime)
See sub-workflows for specific differences
low_mem : :obj:`bool`
Write uncompressed .nii files in some cases to reduce memory usage
name : :obj:`str`
Name of workflow
omp_nthreads : :obj:`int`
Maximum number of threads an individual process may use
output_dir : :obj:`str`
Directory in which to save derivatives
skull_strip_fixed_seed : :obj:`bool`
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1
skull_strip_mode : :obj:`str`
Determiner for T1-weighted skull stripping (`force` ensures skull stripping,
`skip` ignores skull stripping, and `auto` automatically ignores skull stripping
if pre-stripped brains are detected).
skull_strip_template : :py:class:`~niworkflows.utils.spaces.Reference`
Spatial reference to use in atlas-based brain extraction.
spaces : :py:class:`~niworkflows.utils.spaces.SpatialReferences`
Object containing standard and nonstandard space specifications.
subject_id : :obj:`str`
List of subject labels
bids_filters : dict
Provides finer specification of the pipeline input files through pybids entities filters.
A dict with the following structure {<suffix>:{<entity>:<filter>,...},...}
Inputs
------
subjects_dir
FreeSurfer SUBJECTS_DIR
"""
from ..interfaces.reports import AboutSummary, SubjectSummary
if name in ('single_subject_wf', 'single_subject_smripreptest_wf'):
# for documentation purposes
subject_data = {
't1w': ['/completely/made/up/path/sub-01_T1w.nii.gz'],
}
else:
subject_data = collect_data(layout,
subject_id,
bids_filters=bids_filters)[0]
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *sMRIPprep* {smriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(smriprep_ver=__version__, nipype_ver=nipype_ver)
workflow.__postdesc__ = """
For more details of the pipeline, see [the section corresponding
to workflows in *sMRIPrep*'s documentation]\
(https://smriprep.readthedocs.io/en/latest/workflows.html \
"sMRIPrep's documentation").
### References
"""
deriv_cache = None
if fast_track:
from ..utils.bids import collect_derivatives
std_spaces = spaces.get_spaces(nonstandard=False, dim=(3, ))
deriv_cache = collect_derivatives(
Path(output_dir) / 'smriprep', subject_id, std_spaces, freesurfer)
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=True),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(bids_dir=layout.root),
name='bids_info',
run_without_submitting=True)
summary = pe.Node(
SubjectSummary(output_spaces=spaces.get_spaces(nonstandard=False)),
name='summary',
run_without_submitting=True)
about = pe.Node(AboutSummary(version=__version__,
command=' '.join(sys.argv)),
name='about',
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=output_dir,
dismiss_entities=("session", ),
desc='summary',
datatype="figures"),
name='ds_report_summary',
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=output_dir,
dismiss_entities=("session", ),
desc='about',
datatype="figures"),
name='ds_report_about',
run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=layout.root,
debug=debug,
existing_derivatives=deriv_cache,
freesurfer=freesurfer,
hires=hires,
longitudinal=longitudinal,
name="anat_preproc_wf",
t1w=subject_data['t1w'],
omp_nthreads=omp_nthreads,
output_dir=output_dir,
skull_strip_fixed_seed=skull_strip_fixed_seed,
skull_strip_mode=skull_strip_mode,
skull_strip_template=skull_strip_template,
spaces=spaces,
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')
]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix),
'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
return workflow
def init_ica_aroma_wf(template,
metadata,
mem_gb,
omp_nthreads,
name='ica_aroma_wf',
susan_fwhm=6.0,
ignore_aroma_err=False,
aroma_melodic_dim=-200,
use_fieldwarp=True):
"""
This workflow wraps `ICA-AROMA`_ to identify and remove motion-related
independent components from a BOLD time series.
The following steps are performed:
#. Remove non-steady state volumes from the bold series.
#. Smooth data using FSL `susan`, with a kernel width FWHM=6.0mm.
#. Run FSL `melodic` outside of ICA-AROMA to generate the report
#. Run ICA-AROMA
#. Aggregate identified motion components (aggressive) to TSV
#. Return ``classified_motion_ICs`` and ``melodic_mix`` for user to complete
non-aggressive denoising in T1w space
#. Calculate ICA-AROMA-identified noise components
(columns named ``AROMAAggrCompXX``)
Additionally, non-aggressive denoising is performed on the BOLD series
resampled into MNI space.
There is a current discussion on whether other confounds should be extracted
before or after denoising `here <http://nbviewer.jupyter.org/github/poldracklab/\
fmriprep-notebooks/blob/922e436429b879271fa13e76767a6e73443e74d9/issue-817_\
aroma_confounds.ipynb>`__.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.bold.confounds import init_ica_aroma_wf
wf = init_ica_aroma_wf(template='MNI152NLin2009cAsym',
metadata={'RepetitionTime': 1.0},
mem_gb=3,
omp_nthreads=1)
**Parameters**
template : str
Spatial normalization template used as target when that
registration step was previously calculated with
:py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`.
The template must be one of the MNI templates (fMRIPrep uses
``MNI152NLin2009cAsym`` by default).
metadata : dict
BIDS metadata for BOLD file
mem_gb : float
Size of BOLD file in GB
omp_nthreads : int
Maximum number of threads an individual process may use
name : str
Name of workflow (default: ``bold_mni_trans_wf``)
susan_fwhm : float
Kernel width (FWHM in mm) for the smoothing step with
FSL ``susan`` (default: 6.0mm)
use_fieldwarp : bool
Include SDC warp in single-shot transform from BOLD to MNI
ignore_aroma_err : bool
Do not fail on ICA-AROMA errors
aroma_melodic_dim: int
Set the dimensionality of the MELODIC ICA decomposition.
Negative numbers set a maximum on automatic dimensionality estimation.
Positive numbers set an exact number of components to extract.
(default: -200, i.e., estimate <=200 components)
**Inputs**
itk_bold_to_t1
Affine transform from ``ref_bold_brain`` to T1 space (ITK format)
t1_2_mni_forward_transform
ANTs-compatible affine-and-warp transform file
name_source
BOLD series NIfTI file
Used to recover original information lost during processing
skip_vols
number of non steady state volumes
bold_split
Individual 3D BOLD volumes, not motion corrected
bold_mask
BOLD series mask in template space
hmc_xforms
List of affine transforms aligning each volume to ``ref_image`` in ITK format
fieldwarp
a :abbr:`DFM (displacements field map)` in ITK format
movpar_file
SPM-formatted motion parameters file
**Outputs**
aroma_confounds
TSV of confounds identified as noise by ICA-AROMA
aroma_noise_ics
CSV of noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
nonaggr_denoised_file
BOLD series with non-aggressive ICA-AROMA denoising applied
.. _ICA-AROMA: https://github.com/maartenmennes/ICA-AROMA
"""
workflow = Workflow(name=name)
workflow.__postdesc__ = """\
Automatic removal of motion artifacts using independent component analysis
[ICA-AROMA, @aroma] was performed on the *preprocessed BOLD on MNI space*
time-series after removal of non-steady state volumes and spatial smoothing
with an isotropic, Gaussian kernel of 6mm FWHM (full-width half-maximum).
Corresponding "non-aggresively" denoised runs were produced after such
smoothing.
Additionally, the "aggressive" noise-regressors were collected and placed
in the corresponding confounds file.
"""
inputnode = pe.Node(niu.IdentityInterface(fields=[
'itk_bold_to_t1', 't1_2_mni_forward_transform', 'name_source',
'skip_vols', 'bold_split', 'bold_mask', 'hmc_xforms', 'fieldwarp',
'movpar_file'
]),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(fields=[
'aroma_confounds', 'aroma_noise_ics', 'melodic_mix',
'nonaggr_denoised_file'
]),
name='outputnode')
bold_mni_trans_wf = init_bold_mni_trans_wf(
template=template,
freesurfer=False,
mem_gb=mem_gb,
omp_nthreads=omp_nthreads,
template_out_grid=str(
get_template('MNI152Lin') /
'tpl-MNI152Lin_space-MNI_res-02_T1w.nii.gz'),
use_compression=False,
use_fieldwarp=use_fieldwarp,
name='bold_mni_trans_wf')
bold_mni_trans_wf.__desc__ = None
rm_non_steady_state = pe.Node(niu.Function(function=_remove_volumes,
output_names=['bold_cut']),
name='rm_nonsteady')
calc_median_val = pe.Node(fsl.ImageStats(op_string='-k %s -p 50'),
name='calc_median_val')
calc_bold_mean = pe.Node(fsl.MeanImage(), name='calc_bold_mean')
def _getusans_func(image, thresh):
return [tuple([image, thresh])]
getusans = pe.Node(niu.Function(function=_getusans_func,
output_names=['usans']),
name='getusans',
mem_gb=0.01)
smooth = pe.Node(fsl.SUSAN(fwhm=susan_fwhm), name='smooth')
# melodic node
melodic = pe.Node(fsl.MELODIC(no_bet=True,
tr_sec=float(metadata['RepetitionTime']),
mm_thresh=0.5,
out_stats=True,
dim=aroma_melodic_dim),
name="melodic")
# ica_aroma node
ica_aroma = pe.Node(ICA_AROMARPT(denoise_type='nonaggr',
generate_report=True,
TR=metadata['RepetitionTime']),
name='ica_aroma')
add_non_steady_state = pe.Node(niu.Function(function=_add_volumes,
output_names=['bold_add']),
name='add_nonsteady')
# extract the confound ICs from the results
ica_aroma_confound_extraction = pe.Node(
ICAConfounds(ignore_aroma_err=ignore_aroma_err),
name='ica_aroma_confound_extraction')
ds_report_ica_aroma = pe.Node(DerivativesDataSink(suffix='ica_aroma'),
name='ds_report_ica_aroma',
run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
def _getbtthresh(medianval):
return 0.75 * medianval
# connect the nodes
workflow.connect([
(inputnode, bold_mni_trans_wf,
[('name_source', 'inputnode.name_source'),
('bold_split', 'inputnode.bold_split'),
('bold_mask', 'inputnode.bold_mask'),
('hmc_xforms', 'inputnode.hmc_xforms'),
('itk_bold_to_t1', 'inputnode.itk_bold_to_t1'),
('t1_2_mni_forward_transform',
'inputnode.t1_2_mni_forward_transform'),
('fieldwarp', 'inputnode.fieldwarp')]),
(inputnode, ica_aroma, [('movpar_file', 'motion_parameters')]),
(inputnode, rm_non_steady_state, [('skip_vols', 'skip_vols')]),
(bold_mni_trans_wf, rm_non_steady_state, [('outputnode.bold_mni',
'bold_file')]),
(bold_mni_trans_wf, calc_median_val, [('outputnode.bold_mask_mni',
'mask_file')]),
(rm_non_steady_state, calc_median_val, [('bold_cut', 'in_file')]),
(rm_non_steady_state, calc_bold_mean, [('bold_cut', 'in_file')]),
(calc_bold_mean, getusans, [('out_file', 'image')]),
(calc_median_val, getusans, [('out_stat', 'thresh')]),
# Connect input nodes to complete smoothing
(rm_non_steady_state, smooth, [('bold_cut', 'in_file')]),
(getusans, smooth, [('usans', 'usans')]),
(calc_median_val, smooth, [(('out_stat', _getbtthresh),
'brightness_threshold')]),
# connect smooth to melodic
(smooth, melodic, [('smoothed_file', 'in_files')]),
(bold_mni_trans_wf, melodic, [('outputnode.bold_mask_mni', 'mask')]),
# connect nodes to ICA-AROMA
(smooth, ica_aroma, [('smoothed_file', 'in_file')]),
(bold_mni_trans_wf, ica_aroma, [('outputnode.bold_mask_mni',
'report_mask'),
('outputnode.bold_mask_mni', 'mask')]),
(melodic, ica_aroma, [('out_dir', 'melodic_dir')]),
# generate tsvs from ICA-AROMA
(ica_aroma, ica_aroma_confound_extraction, [('out_dir', 'in_directory')
]),
(inputnode, ica_aroma_confound_extraction, [('skip_vols', 'skip_vols')
]),
# output for processing and reporting
(ica_aroma_confound_extraction,
outputnode, [('aroma_confounds', 'aroma_confounds'),
('aroma_noise_ics', 'aroma_noise_ics'),
('melodic_mix', 'melodic_mix')]),
# TODO change melodic report to reflect noise and non-noise components
(ica_aroma, add_non_steady_state, [('nonaggr_denoised_file',
'bold_cut_file')]),
(bold_mni_trans_wf, add_non_steady_state, [('outputnode.bold_mni',
'bold_file')]),
(inputnode, add_non_steady_state, [('skip_vols', 'skip_vols')]),
(add_non_steady_state, outputnode, [('bold_add',
'nonaggr_denoised_file')]),
(ica_aroma, ds_report_ica_aroma, [('out_report', 'in_file')]),
])
return workflow
def init_func_preproc_wf(
aroma_melodic_dim,
bold2t1w_dof,
bold_file,
cifti_output,
debug,
dummy_scans,
err_on_aroma_warn,
fmap_bspline,
fmap_demean,
force_syn,
freesurfer,
ignore,
low_mem,
medial_surface_nan,
omp_nthreads,
output_dir,
output_spaces,
regressors_all_comps,
regressors_dvars_th,
regressors_fd_th,
reportlets_dir,
t2s_coreg,
use_aroma,
use_bbr,
use_syn,
layout=None,
num_bold=1,
):
"""
This workflow controls the functional preprocessing stages of FMRIPREP.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.bold import init_func_preproc_wf
from collections import namedtuple, OrderedDict
BIDSLayout = namedtuple('BIDSLayout', ['root'])
wf = init_func_preproc_wf(
aroma_melodic_dim=-200,
bold2t1w_dof=9,
bold_file='/completely/made/up/path/sub-01_task-nback_bold.nii.gz',
cifti_output=False,
debug=False,
dummy_scans=None,
err_on_aroma_warn=False,
fmap_bspline=True,
fmap_demean=True,
force_syn=True,
freesurfer=True,
ignore=[],
low_mem=False,
medial_surface_nan=False,
omp_nthreads=1,
output_dir='.',
output_spaces=OrderedDict([
('MNI152Lin', {}), ('fsaverage', {'density': '10k'}),
('T1w', {}), ('fsnative', {})]),
regressors_all_comps=False,
regressors_dvars_th=1.5,
regressors_fd_th=0.5,
reportlets_dir='.',
t2s_coreg=False,
use_aroma=False,
use_bbr=True,
use_syn=True,
layout=BIDSLayout('.'),
num_bold=1,
)
**Parameters**
aroma_melodic_dim : int
Maximum number of components identified by MELODIC within ICA-AROMA
(default is -200, ie. no limitation).
bold2t1w_dof : 6, 9 or 12
Degrees-of-freedom for BOLD-T1w registration
bold_file : str
BOLD series NIfTI file
cifti_output : bool
Generate bold CIFTI file in output spaces
debug : bool
Enable debugging outputs
dummy_scans : int or None
Number of volumes to consider as non steady state
err_on_aroma_warn : bool
Do not crash on ICA-AROMA errors
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
force_syn : bool
**Temporary**: Always run SyN-based SDC
freesurfer : bool
Enable FreeSurfer functional registration (bbregister) and resampling
BOLD series to FreeSurfer surface meshes.
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
medial_surface_nan : bool
Replace medial wall values with NaNs on functional GIFTI files
omp_nthreads : int
Maximum number of threads an individual process may use
output_dir : str
Directory in which to save derivatives
output_spaces : OrderedDict
Ordered dictionary where keys are TemplateFlow ID strings (e.g. ``MNI152Lin``,
``MNI152NLin6Asym``, ``MNI152NLin2009cAsym``, or ``fsLR``) strings designating
nonstandard references (e.g. ``T1w`` or ``anat``, ``sbref``, ``run``, etc.),
or paths pointing to custom templates organized in a TemplateFlow-like structure.
Values of the dictionary aggregate modifiers (e.g. the value for the key ``MNI152Lin``
could be ``{'resolution': 2}`` if one wants the resampling to be done on the 2mm
resolution version of the selected template).
regressors_all_comps
Return all CompCor component time series instead of the top fraction
regressors_dvars_th
Criterion for flagging DVARS outliers
regressors_fd_th
Criterion for flagging framewise displacement outliers
reportlets_dir : str
Absolute path of a directory in which reportlets will be temporarily stored
t2s_coreg : bool
For multiecho EPI, use the calculated T2*-map for T2*-driven coregistration
use_aroma : bool
Perform ICA-AROMA on MNI-resampled functional series
use_bbr : bool or None
Enable/disable boundary-based registration refinement.
If ``None``, test BBR result for distortion before accepting.
When using ``t2s_coreg``, BBR will be enabled by default unless
explicitly specified otherwise.
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
layout : BIDSLayout
BIDSLayout structure to enable metadata retrieval
num_bold : int
Total number of BOLD files that have been set for preprocessing
(default is 1)
**Inputs**
bold_file
BOLD series NIfTI file
t1_preproc
Bias-corrected structural template image
t1_brain
Skull-stripped ``t1_preproc``
t1_mask
Mask of the skull-stripped template image
t1_seg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1_tpms
List of tissue probability maps in T1w space
anat2std_xfm
ANTs-compatible affine-and-warp transform file
std2anat_xfm
ANTs-compatible affine-and-warp transform file (inverse)
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1_2_fsnative_forward_transform
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
t1_2_fsnative_reverse_transform
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
**Outputs**
bold_t1
BOLD series, resampled to T1w space
bold_mask_t1
BOLD series mask in T1w space
bold_std
BOLD series, resampled to template space
bold_mask_std
BOLD series mask in template space
confounds
TSV of confounds
surfaces
BOLD series, resampled to FreeSurfer surfaces
aroma_noise_ics
Noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
bold_cifti
BOLD CIFTI image
cifti_variant
combination of target spaces for `bold_cifti`
**Subworkflows**
* :py:func:`~fmriprep.workflows.bold.util.init_bold_reference_wf`
* :py:func:`~fmriprep.workflows.bold.stc.init_bold_stc_wf`
* :py:func:`~fmriprep.workflows.bold.hmc.init_bold_hmc_wf`
* :py:func:`~fmriprep.workflows.bold.t2s.init_bold_t2s_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_t1_trans_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_bold_confounds_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_ica_aroma_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_std_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_preproc_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_surf_wf`
* :py:func:`~fmriprep.workflows.fieldmap.pepolar.init_pepolar_unwarp_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_fmap_estimator_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_sdc_unwarp_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_nonlinear_sdc_wf`
"""
from .resampling import NONSTANDARD_REFERENCES
from ..fieldmap.base import init_sdc_wf # Avoid circular dependency (#1066)
# Filter out standard spaces to a separate dict
std_spaces = OrderedDict([(key, modifiers)
for key, modifiers in output_spaces.items()
if key not in NONSTANDARD_REFERENCES])
volume_std_spaces = OrderedDict([(key, modifiers)
for key, modifiers in std_spaces.items()
if not key.startswith('fs')])
ref_file = bold_file
mem_gb = {'filesize': 1, 'resampled': 1, 'largemem': 1}
bold_tlen = 10
multiecho = isinstance(bold_file, list)
if multiecho:
tes = [layout.get_metadata(echo)['EchoTime'] for echo in bold_file]
ref_file = dict(zip(tes, bold_file))[min(tes)]
if os.path.isfile(ref_file):
bold_tlen, mem_gb = _create_mem_gb(ref_file)
wf_name = _get_wf_name(ref_file)
LOGGER.log(
25, ('Creating bold processing workflow for "%s" (%.2f GB / %d TRs). '
'Memory resampled/largemem=%.2f/%.2f GB.'), ref_file,
mem_gb['filesize'], bold_tlen, mem_gb['resampled'], mem_gb['largemem'])
sbref_file = None
# For doc building purposes
if not hasattr(layout, 'parse_file_entities'):
LOGGER.log(25, 'No valid layout: building empty workflow.')
metadata = {
'RepetitionTime': 2.0,
'SliceTiming': [0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9],
'PhaseEncodingDirection': 'j',
}
fmaps = [{
'suffix':
'phasediff',
'phasediff':
'sub-03/ses-2/fmap/sub-03_ses-2_run-1_phasediff.nii.gz',
'magnitude1':
'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude1.nii.gz',
'magnitude2':
'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude2.nii.gz',
}]
run_stc = True
multiecho = False
else:
# Find associated sbref, if possible
entities = layout.parse_file_entities(ref_file)
entities['suffix'] = 'sbref'
entities['extension'] = ['nii', 'nii.gz'] # Overwrite extensions
files = layout.get(return_type='file', **entities)
refbase = os.path.basename(ref_file)
if 'sbref' in ignore:
LOGGER.info("Single-band reference files ignored.")
elif files and multiecho:
LOGGER.warning("Single-band reference found, but not supported in "
"multi-echo workflows at this time. Ignoring.")
elif files:
sbref_file = files[0]
sbbase = os.path.basename(sbref_file)
if len(files) > 1:
LOGGER.warning(
"Multiple single-band reference files found for {}; using "
"{}".format(refbase, sbbase))
else:
LOGGER.log(
25, "Using single-band reference file {}".format(sbbase))
else:
LOGGER.log(25,
"No single-band-reference found for {}".format(refbase))
metadata = layout.get_metadata(ref_file)
# Find fieldmaps. Options: (phase1|phase2|phasediff|epi|fieldmap|syn)
fmaps = []
if 'fieldmaps' not in ignore:
for fmap in layout.get_fieldmap(ref_file, return_list=True):
if fmap['suffix'] == 'phase':
LOGGER.warning("""\
Found phase1/2 type of fieldmaps, which are not currently supported. \
fMRIPrep will discard them for susceptibility distortion correction. \
Please, follow up on this issue at \
https://github.com/poldracklab/fmriprep/issues/1655.""")
else:
fmap['metadata'] = layout.get_metadata(
fmap[fmap['suffix']])
fmaps.append(fmap)
# Run SyN if forced or in the absence of fieldmap correction
if force_syn or (use_syn and not fmaps):
fmaps.append({'suffix': 'syn'})
# Short circuits: (True and True and (False or 'TooShort')) == 'TooShort'
run_stc = ("SliceTiming" in metadata and 'slicetiming' not in ignore
and (_get_series_len(ref_file) > 4 or "TooShort"))
# Check if MEEPI for T2* coregistration target
if t2s_coreg and not multiecho:
LOGGER.warning(
"No multiecho BOLD images found for T2* coregistration. "
"Using standard EPI-T1 coregistration.")
t2s_coreg = False
# By default, force-bbr for t2s_coreg unless user specifies otherwise
if t2s_coreg and use_bbr is None:
use_bbr = True
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__desc__ = """
Functional data preprocessing
: For each of the {num_bold} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""".format(num_bold=num_bold)
workflow.__postdesc__ = """\
All resamplings can be performed with *a single interpolation
step* by composing all the pertinent transformations (i.e. head-motion
transform matrices, susceptibility distortion correction when available,
and co-registrations to anatomical and output spaces).
Gridded (volumetric) resamplings were performed using `antsApplyTransforms` (ANTs),
configured with Lanczos interpolation to minimize the smoothing
effects of other kernels [@lanczos].
Non-gridded (surface) resamplings were performed using `mri_vol2surf`
(FreeSurfer).
"""
inputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_file', 'subjects_dir', 'subject_id', 't1_preproc', 't1_brain',
't1_mask', 't1_seg', 't1_tpms', 't1_aseg', 't1_aparc', 'anat2std_xfm',
'std2anat_xfm', 'template', 'joint_anat2std_xfm', 'joint_std2anat_xfm',
'joint_template', 't1_2_fsnative_forward_transform',
't1_2_fsnative_reverse_transform'
]),
name='inputnode')
inputnode.inputs.bold_file = bold_file
if sbref_file is not None:
from niworkflows.interfaces.images import ValidateImage
val_sbref = pe.Node(ValidateImage(in_file=sbref_file),
name='val_sbref')
outputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_t1', 'bold_t1_ref', 'bold_mask_t1', 'bold_aseg_t1',
'bold_aparc_t1', 'bold_std', 'bold_std_ref'
'bold_mask_std', 'bold_aseg_std', 'bold_aparc_std', 'bold_native',
'bold_cifti', 'cifti_variant', 'cifti_variant_key', 'surfaces',
'confounds', 'aroma_noise_ics', 'melodic_mix', 'nonaggr_denoised_file',
'confounds_metadata'
]),
name='outputnode')
# BOLD buffer: an identity used as a pointer to either the original BOLD
# or the STC'ed one for further use.
boldbuffer = pe.Node(niu.IdentityInterface(fields=['bold_file']),
name='boldbuffer')
summary = pe.Node(FunctionalSummary(
slice_timing=run_stc,
registration=('FSL', 'FreeSurfer')[freesurfer],
registration_dof=bold2t1w_dof,
pe_direction=metadata.get("PhaseEncodingDirection"),
tr=metadata.get("RepetitionTime")),
name='summary',
mem_gb=DEFAULT_MEMORY_MIN_GB,
run_without_submitting=True)
summary.inputs.dummy_scans = dummy_scans
# CIfTI output: currently, we only support fsaverage{5,6}
cifti_spaces = set(s for s in output_spaces.keys()
if s in ('fsaverage5', 'fsaverage6'))
fsaverage_den = output_spaces.get('fsaverage', {}).get('den')
if fsaverage_den:
cifti_spaces.add(FSAVERAGE_DENSITY[fsaverage_den])
cifti_output = cifti_output and cifti_spaces
func_derivatives_wf = init_func_derivatives_wf(
bids_root=layout.root,
cifti_output=cifti_output,
freesurfer=freesurfer,
metadata=metadata,
output_dir=output_dir,
output_spaces=output_spaces,
standard_spaces=list(std_spaces.keys()),
use_aroma=use_aroma,
)
workflow.connect([
(outputnode, func_derivatives_wf, [
('bold_t1', 'inputnode.bold_t1'),
('bold_t1_ref', 'inputnode.bold_t1_ref'),
('bold_aseg_t1', 'inputnode.bold_aseg_t1'),
('bold_aparc_t1', 'inputnode.bold_aparc_t1'),
('bold_mask_t1', 'inputnode.bold_mask_t1'),
('bold_native', 'inputnode.bold_native'),
('confounds', 'inputnode.confounds'),
('surfaces', 'inputnode.surfaces'),
('aroma_noise_ics', 'inputnode.aroma_noise_ics'),
('melodic_mix', 'inputnode.melodic_mix'),
('nonaggr_denoised_file', 'inputnode.nonaggr_denoised_file'),
('bold_cifti', 'inputnode.bold_cifti'),
('cifti_variant', 'inputnode.cifti_variant'),
('cifti_variant_key', 'inputnode.cifti_variant_key'),
('confounds_metadata', 'inputnode.confounds_metadata'),
]),
])
# Generate a tentative boldref
bold_reference_wf = init_bold_reference_wf(omp_nthreads=omp_nthreads)
bold_reference_wf.inputs.inputnode.dummy_scans = dummy_scans
if sbref_file is not None:
workflow.connect([
(val_sbref, bold_reference_wf, [('out_file',
'inputnode.sbref_file')]),
])
# Top-level BOLD splitter
bold_split = pe.Node(FSLSplit(dimension='t'),
name='bold_split',
mem_gb=mem_gb['filesize'] * 3)
# HMC on the BOLD
bold_hmc_wf = init_bold_hmc_wf(name='bold_hmc_wf',
mem_gb=mem_gb['filesize'],
omp_nthreads=omp_nthreads)
# calculate BOLD registration to T1w
bold_reg_wf = init_bold_reg_wf(name='bold_reg_wf',
freesurfer=freesurfer,
use_bbr=use_bbr,
bold2t1w_dof=bold2t1w_dof,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# apply BOLD registration to T1w
bold_t1_trans_wf = init_bold_t1_trans_wf(name='bold_t1_trans_wf',
freesurfer=freesurfer,
use_fieldwarp=(fmaps is not None
or use_syn),
multiecho=multiecho,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# get confounds
bold_confounds_wf = init_bold_confs_wf(
mem_gb=mem_gb['largemem'],
metadata=metadata,
regressors_all_comps=regressors_all_comps,
regressors_fd_th=regressors_fd_th,
regressors_dvars_th=regressors_dvars_th,
name='bold_confounds_wf')
bold_confounds_wf.get_node('inputnode').inputs.t1_transform_flags = [False]
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_bold_trans_wf = init_bold_preproc_trans_wf(
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=not low_mem,
use_fieldwarp=(fmaps is not None or use_syn),
name='bold_bold_trans_wf')
bold_bold_trans_wf.inputs.inputnode.name_source = ref_file
# SLICE-TIME CORRECTION (or bypass) #############################################
if run_stc is True: # bool('TooShort') == True, so check True explicitly
bold_stc_wf = init_bold_stc_wf(name='bold_stc_wf', metadata=metadata)
workflow.connect([
(bold_reference_wf, bold_stc_wf, [('outputnode.skip_vols',
'inputnode.skip_vols')]),
(bold_stc_wf, boldbuffer, [('outputnode.stc_file', 'bold_file')]),
])
if not multiecho:
workflow.connect([(bold_reference_wf, bold_stc_wf, [
('outputnode.bold_file', 'inputnode.bold_file')
])])
else: # for meepi, iterate through stc_wf for all workflows
meepi_echos = boldbuffer.clone(name='meepi_echos')
meepi_echos.iterables = ('bold_file', bold_file)
workflow.connect([(meepi_echos, bold_stc_wf,
[('bold_file', 'inputnode.bold_file')])])
elif not multiecho: # STC is too short or False
# bypass STC from original BOLD to the splitter through boldbuffer
workflow.connect([(bold_reference_wf, boldbuffer,
[('outputnode.bold_file', 'bold_file')])])
else:
# for meepi, iterate over all meepi echos to boldbuffer
boldbuffer.iterables = ('bold_file', bold_file)
# SDC (SUSCEPTIBILITY DISTORTION CORRECTION) or bypass ##########################
bold_sdc_wf = init_sdc_wf(fmaps,
metadata,
omp_nthreads=omp_nthreads,
debug=debug,
fmap_demean=fmap_demean,
fmap_bspline=fmap_bspline)
# If no standard space is given, use the default for SyN-SDC
if not volume_std_spaces or 'MNI152NLin2009cAsym' in volume_std_spaces:
bold_sdc_wf.inputs.inputnode.template = 'MNI152NLin2009cAsym'
else:
bold_sdc_wf.inputs.inputnode.template = next(iter(volume_std_spaces))
if not fmaps:
LOGGER.warning('SDC: no fieldmaps found or they were ignored (%s).',
ref_file)
elif fmaps[0]['suffix'] == 'syn':
LOGGER.warning(
'SDC: no fieldmaps found or they were ignored. '
'Using EXPERIMENTAL "fieldmap-less SyN" correction '
'for dataset %s.', ref_file)
else:
LOGGER.log(
25, 'SDC: fieldmap estimation of type "%s" intended for %s found.',
fmaps[0]['suffix'], ref_file)
# Overwrite ``out_path_base`` of sdcflows' DataSinks
for node in bold_sdc_wf.list_node_names():
if node.split('.')[-1].startswith('ds_'):
bold_sdc_wf.get_node(node).interface.out_path_base = 'fmriprep'
# MULTI-ECHO EPI DATA #############################################
if multiecho:
from .util import init_skullstrip_bold_wf
skullstrip_bold_wf = init_skullstrip_bold_wf(name='skullstrip_bold_wf')
inputnode.inputs.bold_file = ref_file # Replace reference w first echo
join_echos = pe.JoinNode(
niu.IdentityInterface(fields=['bold_files']),
joinsource=('meepi_echos' if run_stc is True else 'boldbuffer'),
joinfield=['bold_files'],
name='join_echos')
# create optimal combination, adaptive T2* map
bold_t2s_wf = init_bold_t2s_wf(echo_times=tes,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
t2s_coreg=t2s_coreg,
name='bold_t2smap_wf')
workflow.connect([
(skullstrip_bold_wf, join_echos,
[('outputnode.skull_stripped_file', 'bold_files')]),
(join_echos, bold_t2s_wf, [('bold_files', 'inputnode.bold_file')]),
])
# MAIN WORKFLOW STRUCTURE #######################################################
workflow.connect([
# Generate early reference
(inputnode, bold_reference_wf, [('bold_file', 'inputnode.bold_file')]),
# BOLD buffer has slice-time corrected if it was run, original otherwise
(boldbuffer, bold_split, [('bold_file', 'in_file')]),
# HMC
(bold_reference_wf, bold_hmc_wf,
[('outputnode.raw_ref_image', 'inputnode.raw_ref_image'),
('outputnode.bold_file', 'inputnode.bold_file')]),
(bold_reference_wf, summary, [('outputnode.algo_dummy_scans',
'algo_dummy_scans')]),
# EPI-T1 registration workflow
(
inputnode,
bold_reg_wf,
[
('t1_brain', 'inputnode.t1_brain'),
('t1_seg', 'inputnode.t1_seg'),
# Undefined if --no-freesurfer, but this is safe
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1_2_fsnative_reverse_transform',
'inputnode.t1_2_fsnative_reverse_transform')
]),
(inputnode, bold_t1_trans_wf, [('bold_file', 'inputnode.name_source'),
('t1_brain', 'inputnode.t1_brain'),
('t1_mask', 'inputnode.t1_mask'),
('t1_aseg', 'inputnode.t1_aseg'),
('t1_aparc', 'inputnode.t1_aparc')]),
# unused if multiecho, but this is safe
(bold_hmc_wf, bold_t1_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_t1_trans_wf, [('outputnode.itk_bold_to_t1',
'inputnode.itk_bold_to_t1')]),
(bold_t1_trans_wf, outputnode,
[('outputnode.bold_t1', 'bold_t1'),
('outputnode.bold_t1_ref', 'bold_t1_ref'),
('outputnode.bold_aseg_t1', 'bold_aseg_t1'),
('outputnode.bold_aparc_t1', 'bold_aparc_t1')]),
(bold_reg_wf, summary, [('outputnode.fallback', 'fallback')]),
# SDC (or pass-through workflow)
(inputnode, bold_sdc_wf, [('joint_template', 'inputnode.templates'),
('joint_std2anat_xfm',
'inputnode.std2anat_xfm')]),
(inputnode, bold_sdc_wf, [('t1_brain', 'inputnode.t1_brain')]),
(bold_reference_wf, bold_sdc_wf,
[('outputnode.ref_image', 'inputnode.bold_ref'),
('outputnode.ref_image_brain', 'inputnode.bold_ref_brain'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
# For t2s_coreg, replace EPI-to-T1w registration inputs
(bold_sdc_wf if not t2s_coreg else bold_t2s_wf, bold_reg_wf,
[('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain')]),
(bold_sdc_wf if not t2s_coreg else bold_t2s_wf, bold_t1_trans_wf,
[('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain'),
('outputnode.bold_mask', 'inputnode.ref_bold_mask')]),
(bold_sdc_wf, bold_t1_trans_wf, [('outputnode.out_warp',
'inputnode.fieldwarp')]),
(bold_sdc_wf, bold_bold_trans_wf,
[('outputnode.out_warp', 'inputnode.fieldwarp'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, summary, [('outputnode.method', 'distortion_correction')
]),
# Connect bold_confounds_wf
(inputnode, bold_confounds_wf, [('t1_tpms', 'inputnode.t1_tpms'),
('t1_mask', 'inputnode.t1_mask')]),
(bold_hmc_wf, bold_confounds_wf, [('outputnode.movpar_file',
'inputnode.movpar_file')]),
(bold_reg_wf, bold_confounds_wf, [('outputnode.itk_t1_to_bold',
'inputnode.t1_bold_xform')]),
(bold_reference_wf, bold_confounds_wf, [('outputnode.skip_vols',
'inputnode.skip_vols')]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_metadata', 'confounds_metadata'),
]),
# Connect bold_bold_trans_wf
(bold_split, bold_bold_trans_wf, [('out_files', 'inputnode.bold_file')]
),
(bold_hmc_wf, bold_bold_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
# Summary
(outputnode, summary, [('confounds', 'confounds_file')]),
])
# for standard EPI data, pass along correct file
if not multiecho:
workflow.connect([
(inputnode, func_derivatives_wf, [('bold_file',
'inputnode.source_file')]),
(bold_bold_trans_wf, bold_confounds_wf,
[('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_split, bold_t1_trans_wf, [('out_files',
'inputnode.bold_split')]),
])
else: # for meepi, create and use optimal combination
workflow.connect([
# update name source for optimal combination
(inputnode, func_derivatives_wf,
[(('bold_file', combine_meepi_source), 'inputnode.source_file')]),
(bold_bold_trans_wf, skullstrip_bold_wf, [('outputnode.bold',
'inputnode.in_file')]),
(bold_t2s_wf, bold_confounds_wf,
[('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_t2s_wf, bold_t1_trans_wf, [('outputnode.bold',
'inputnode.bold_split')]),
])
if fmaps:
from ..fieldmap.unwarp import init_fmap_unwarp_report_wf
# Report on BOLD correction
fmap_unwarp_report_wf = init_fmap_unwarp_report_wf()
workflow.connect([
(inputnode, fmap_unwarp_report_wf, [('t1_seg', 'inputnode.in_seg')
]),
(bold_reference_wf, fmap_unwarp_report_wf,
[('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, fmap_unwarp_report_wf, [('outputnode.itk_t1_to_bold',
'inputnode.in_xfm')]),
(bold_sdc_wf, fmap_unwarp_report_wf, [('outputnode.bold_ref',
'inputnode.in_post')]),
])
# Overwrite ``out_path_base`` of unwarping DataSinks
for node in fmap_unwarp_report_wf.list_node_names():
if node.split('.')[-1].startswith('ds_'):
fmap_unwarp_report_wf.get_node(
node).interface.out_path_base = 'fmriprep'
if force_syn and fmaps[0]['suffix'] != 'syn':
syn_unwarp_report_wf = init_fmap_unwarp_report_wf(
name='syn_unwarp_report_wf', forcedsyn=True)
workflow.connect([
(inputnode, syn_unwarp_report_wf, [('t1_seg',
'inputnode.in_seg')]),
(bold_reference_wf, syn_unwarp_report_wf,
[('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, syn_unwarp_report_wf,
[('outputnode.itk_t1_to_bold', 'inputnode.in_xfm')]),
(bold_sdc_wf, syn_unwarp_report_wf,
[('outputnode.syn_bold_ref', 'inputnode.in_post')]),
])
# Overwrite ``out_path_base`` of unwarping DataSinks
for node in syn_unwarp_report_wf.list_node_names():
if node.split('.')[-1].startswith('ds_'):
syn_unwarp_report_wf.get_node(
node).interface.out_path_base = 'fmriprep'
# Map final BOLD mask into T1w space (if required)
if 'T1w' in output_spaces or 'anat' in output_spaces:
from niworkflows.interfaces.fixes import (FixHeaderApplyTransforms as
ApplyTransforms)
boldmask_to_t1w = pe.Node(ApplyTransforms(interpolation='MultiLabel',
float=True),
name='boldmask_to_t1w',
mem_gb=0.1)
workflow.connect([
(bold_reg_wf, boldmask_to_t1w, [('outputnode.itk_bold_to_t1',
'transforms')]),
(bold_t1_trans_wf, boldmask_to_t1w, [('outputnode.bold_mask_t1',
'reference_image')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
boldmask_to_t1w, [('outputnode.bold_mask', 'input_image')]),
(boldmask_to_t1w, outputnode, [('output_image', 'bold_mask_t1')]),
])
if set(['func', 'run', 'bold', 'boldref',
'sbref']).intersection(output_spaces):
workflow.connect([
(bold_bold_trans_wf, outputnode, [('outputnode.bold',
'bold_native')]),
(bold_bold_trans_wf, func_derivatives_wf,
[('outputnode.bold_ref', 'inputnode.bold_native_ref'),
('outputnode.bold_mask', 'inputnode.bold_mask_native')]),
])
if volume_std_spaces:
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_std_trans_wf = init_bold_std_trans_wf(
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
standard_spaces=volume_std_spaces,
name='bold_std_trans_wf',
use_compression=not low_mem,
use_fieldwarp=fmaps is not None,
)
workflow.connect([
(inputnode, bold_std_trans_wf,
[('joint_template', 'inputnode.templates'),
('joint_anat2std_xfm', 'inputnode.anat2std_xfm'),
('bold_file', 'inputnode.name_source'),
('t1_aseg', 'inputnode.bold_aseg'),
('t1_aparc', 'inputnode.bold_aparc')]),
(bold_hmc_wf, bold_std_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_std_trans_wf, [('outputnode.itk_bold_to_t1',
'inputnode.itk_bold_to_t1')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
bold_std_trans_wf, [('outputnode.bold_mask',
'inputnode.bold_mask')]),
(bold_sdc_wf, bold_std_trans_wf, [('outputnode.out_warp',
'inputnode.fieldwarp')]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_std', 'bold_std'),
('outputnode.bold_std_ref', 'bold_std_ref'),
('outputnode.bold_mask_std', 'bold_mask_std')]),
])
if freesurfer:
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('poutputnode.bold_aseg_std', 'inputnode.bold_aseg_std'),
('poutputnode.bold_aparc_std', 'inputnode.bold_aparc_std'),
]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_aseg_std', 'bold_aseg_std'),
('outputnode.bold_aparc_std', 'bold_aparc_std')]),
])
if 'MNI152NLin2009cAsym' in std_spaces:
carpetplot_wf = init_carpetplot_wf(standard_spaces=std_spaces,
mem_gb=mem_gb['resampled'],
metadata=metadata,
name='carpetplot_wf')
workflow.connect([
(inputnode, carpetplot_wf, [('joint_std2anat_xfm',
'inputnode.std2anat_xfm')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
carpetplot_wf, [('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask',
'inputnode.bold_mask')]),
(bold_reg_wf, carpetplot_wf, [('outputnode.itk_t1_to_bold',
'inputnode.t1_bold_xform')]),
(bold_confounds_wf, carpetplot_wf,
[('outputnode.confounds_file', 'inputnode.confounds_file')]),
])
if not multiecho:
workflow.connect([(bold_split, bold_std_trans_wf,
[('out_files', 'inputnode.bold_split')])])
else:
split_opt_comb = bold_split.clone(name='split_opt_comb')
workflow.connect([(bold_t2s_wf, split_opt_comb,
[('outputnode.bold', 'in_file')]),
(split_opt_comb, bold_std_trans_wf,
[('out_files', 'inputnode.bold_split')])])
# Artifacts resampled in MNI space can only be sinked if they
# were actually generated. See #1348.
# Uses the parameterized outputnode to generate all outputs
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('poutputnode.templates', 'inputnode.template'),
('poutputnode.bold_std_ref', 'inputnode.bold_std_ref'),
('poutputnode.bold_std', 'inputnode.bold_std'),
('poutputnode.bold_mask_std', 'inputnode.bold_mask_std'),
]),
])
if use_aroma and 'MNI152NLin6Asym' in std_spaces: # ICA-AROMA workflow
from .confounds import init_ica_aroma_wf
ica_aroma_wf = init_ica_aroma_wf(
metadata=metadata,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_fieldwarp=fmaps is not None,
err_on_aroma_warn=err_on_aroma_warn,
aroma_melodic_dim=aroma_melodic_dim,
name='ica_aroma_wf')
join = pe.Node(niu.Function(output_names=["out_file"],
function=_to_join),
name='aroma_confounds')
mrg_conf_metadata = pe.Node(niu.Merge(2),
name='merge_confound_metadata',
run_without_submitting=True)
mrg_conf_metadata2 = pe.Node(DictMerge(),
name='merge_confound_metadata2',
run_without_submitting=True)
workflow.disconnect([
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_metadata', 'confounds_metadata'),
]),
])
workflow.connect([
(bold_std_trans_wf, ica_aroma_wf,
[('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
('outputnode.templates', 'inputnode.templates')]),
(inputnode, ica_aroma_wf, [('bold_file',
'inputnode.name_source')]),
(bold_hmc_wf, ica_aroma_wf, [('outputnode.movpar_file',
'inputnode.movpar_file')]),
(bold_reference_wf, ica_aroma_wf, [('outputnode.skip_vols',
'inputnode.skip_vols')]),
(bold_confounds_wf, join, [('outputnode.confounds_file',
'in_file')]),
(bold_confounds_wf, mrg_conf_metadata,
[('outputnode.confounds_metadata', 'in1')]),
(ica_aroma_wf, join, [('outputnode.aroma_confounds',
'join_file')]),
(ica_aroma_wf, mrg_conf_metadata,
[('outputnode.aroma_metadata', 'in2')]),
(mrg_conf_metadata, mrg_conf_metadata2, [('out', 'in_dicts')]),
(ica_aroma_wf, outputnode,
[('outputnode.aroma_noise_ics', 'aroma_noise_ics'),
('outputnode.melodic_mix', 'melodic_mix'),
('outputnode.nonaggr_denoised_file', 'nonaggr_denoised_file')
]),
(join, outputnode, [('out_file', 'confounds')]),
(mrg_conf_metadata2, outputnode, [('out_dict',
'confounds_metadata')]),
])
# SURFACES ##################################################################################
surface_spaces = [
space for space in output_spaces.keys() if space.startswith('fs')
]
if freesurfer and surface_spaces:
LOGGER.log(25, 'Creating BOLD surface-sampling workflow.')
bold_surf_wf = init_bold_surf_wf(mem_gb=mem_gb['resampled'],
output_spaces=surface_spaces,
medial_surface_nan=medial_surface_nan,
name='bold_surf_wf')
workflow.connect([
(inputnode, bold_surf_wf,
[('t1_preproc', 'inputnode.t1_preproc'),
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1_2_fsnative_forward_transform',
'inputnode.t1_2_fsnative_forward_transform')]),
(bold_t1_trans_wf, bold_surf_wf, [('outputnode.bold_t1',
'inputnode.source_file')]),
(bold_surf_wf, outputnode, [('outputnode.surfaces', 'surfaces')]),
])
if cifti_output:
from niworkflows.interfaces.utility import KeySelect
bold_surf_wf.__desc__ += """\
*Grayordinates* files [@hcppipelines], which combine surface-sampled
data and volume-sampled data, were also generated.
"""
select_std = pe.Node(KeySelect(fields=['bold_std']),
name='select_std',
run_without_submitting=True)
select_std.inputs.key = 'MNI152NLin2009cAsym'
gen_cifti = pe.MapNode(GenerateCifti(),
iterfield=["surface_target", "gifti_files"],
name="gen_cifti")
gen_cifti.inputs.TR = metadata.get("RepetitionTime")
gen_cifti.inputs.surface_target = list(cifti_spaces)
workflow.connect([
(bold_std_trans_wf, select_std,
[('outputnode.templates', 'keys'),
('outputnode.bold_std', 'bold_std')]),
(bold_surf_wf, gen_cifti, [('outputnode.surfaces',
'gifti_files')]),
(inputnode, gen_cifti, [('subjects_dir', 'subjects_dir')]),
(select_std, gen_cifti, [('bold_std', 'bold_file')]),
(gen_cifti, outputnode, [('out_file', 'bold_cifti'),
('variant', 'cifti_variant'),
('variant_key', 'cifti_variant_key')
]),
])
# REPORTING ############################################################
ds_report_summary = pe.Node(DerivativesDataSink(desc='summary',
keep_dtype=True),
name='ds_report_summary',
run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
ds_report_validation = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='validation', keep_dtype=True),
name='ds_report_validation',
run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
workflow.connect([
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bold_reference_wf, ds_report_validation,
[('outputnode.validation_report', 'in_file')]),
])
# Fill-in datasinks of reportlets seen so far
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_report'):
workflow.get_node(node).inputs.base_directory = reportlets_dir
workflow.get_node(node).inputs.source_file = ref_file
return workflow
def init_single_subject_wf(
anat_only,
debug,
force_syn,
freesurfer,
hires,
ignore,
layout,
longitudinal,
low_mem,
name,
omp_nthreads,
output_dir,
output_spaces,
reportlets_dir,
skull_strip_fixed_seed,
skull_strip_template,
subject_id,
use_syn,
):
"""
Set-up the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and diffusion MRI preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Diffusion MRI preprocessing is performed using a separate workflow for
a full :abbr:`DWI (diffusion weighted imaging)` *entity*.
A DWI *entity* may comprehend one or several runs (for instance, two
opposed :abbr:`PE (phase-encoding)` directions.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from dmriprep.workflows.base import init_single_subject_wf
from collections import namedtuple, OrderedDict
BIDSLayout = namedtuple('BIDSLayout', ['root'])
wf = init_single_subject_wf(
anat_only=False,
debug=False,
force_syn=True,
freesurfer=True,
hires=True,
ignore=[],
layout=BIDSLayout('.'),
longitudinal=False,
low_mem=False,
name='single_subject_wf',
omp_nthreads=1,
output_dir='.',
output_spaces=OrderedDict([
('MNI152Lin', {}), ('fsaverage', {'density': '10k'}),
('T1w', {}), ('fsnative', {})]),
reportlets_dir='.',
skull_strip_fixed_seed=False,
skull_strip_template=('OASIS30ANTs', {}),
subject_id='test',
use_syn=True,
)
Parameters
----------
anat_only : bool
Disable diffusion MRI workflows
debug : bool
Enable debugging outputs
force_syn : bool
**Temporary**: Always run SyN-based SDC
freesurfer : bool
Enable FreeSurfer surface reconstruction (may increase runtime)
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
layout : BIDSLayout object
BIDS dataset layout
longitudinal : bool
Treat multiple sessions as longitudinal (may increase runtime)
See sub-workflows for specific differences
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
name : str
Name of workflow
omp_nthreads : int
Maximum number of threads an individual process may use
output_dir : str
Directory in which to save derivatives
output_spaces : OrderedDict
Ordered dictionary where keys are TemplateFlow ID strings (e.g., ``MNI152Lin``,
``MNI152NLin6Asym``, ``MNI152NLin2009cAsym``, or ``fsLR``) strings designating
nonstandard references (e.g., ``T1w`` or ``anat``, ``sbref``, ``run``, etc.),
or paths pointing to custom templates organized in a TemplateFlow-like structure.
Values of the dictionary aggregate modifiers (e.g., the value for the key ``MNI152Lin``
could be ``{'resolution': 2}`` if one wants the resampling to be done on the 2mm
resolution version of the selected template).
reportlets_dir : str
Directory in which to save reportlets
skull_strip_fixed_seed : bool
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1
skull_strip_template : tuple
Name of target template for brain extraction with ANTs' ``antsBrainExtraction``,
and corresponding dictionary of output-space modifiers.
subject_id : str
List of subject labels
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
Inputs
------
subjects_dir : os.pathlike
FreeSurfer's ``$SUBJECTS_DIR``
"""
from ..config import NONSTANDARD_REFERENCES
if name in ('single_subject_wf', 'single_subject_dmripreptest_wf'):
# for documentation purposes
subject_data = {
't1w': ['/completely/made/up/path/sub-01_T1w.nii.gz'],
'dwi': ['/completely/made/up/path/sub-01_dwi.nii.gz']
}
else:
subject_data = collect_data(layout, subject_id)[0]
# Make sure we always go through these two checks
if not anat_only and subject_data['dwi'] == []:
raise Exception("No DWI data found for participant {}. "
"All workflows require DWI images.".format(subject_id))
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *dMRIPrep* {dmriprep_ver}
(@dmriprep; RRID:SCR_017412),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(dmriprep_ver=__version__, nipype_ver=nipype_ver)
workflow.__postdesc__ = """
For more details of the pipeline, see [the section corresponding
to workflows in *dMRIPrep*'s documentation]\
(https://nipreps.github.io/dmriprep/{dmriprep_ver}/workflows.html \
"dMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by dMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(dmriprep_ver=Version(__version__).public)
# Filter out standard spaces to a separate dict
std_spaces = OrderedDict([(key, modifiers)
for key, modifiers in output_spaces.items()
if key not in NONSTANDARD_REFERENCES])
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(bids_dir=layout.root, bids_validate=False),
name='bids_info')
summary = pe.Node(SubjectSummary(
std_spaces=list(std_spaces.keys()),
nstd_spaces=list(
set(NONSTANDARD_REFERENCES).intersection(output_spaces.keys()))),
name='summary',
run_without_submitting=True)
about = pe.Node(AboutSummary(version=__version__,
command=' '.join(sys.argv)),
name='about',
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='summary', keep_dtype=True),
name='ds_report_summary',
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='about', keep_dtype=True),
name='ds_report_about',
run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=layout.root,
debug=debug,
freesurfer=freesurfer,
hires=hires,
longitudinal=longitudinal,
name="anat_preproc_wf",
num_t1w=len(subject_data['t1w']),
omp_nthreads=omp_nthreads,
output_dir=output_dir,
output_spaces=std_spaces,
reportlets_dir=reportlets_dir,
skull_strip_fixed_seed=skull_strip_fixed_seed,
skull_strip_template=skull_strip_template,
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')
]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w'), ('dwi', 'dwi')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix),
'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_'):
workflow.get_node(node).interface.out_path_base = 'dmriprep'
if anat_only:
return workflow
for dwi_file in subject_data['dwi']:
dwi_preproc_wf = init_dwi_preproc_wf(
dwi_file=dwi_file,
debug=debug,
force_syn=force_syn,
ignore=ignore,
layout=layout,
low_mem=low_mem,
num_dwi=len(subject_data['dwi']),
omp_nthreads=omp_nthreads,
output_dir=output_dir,
reportlets_dir=reportlets_dir,
use_syn=use_syn,
)
workflow.connect([
(
anat_preproc_wf,
dwi_preproc_wf,
[
(('outputnode.t1w_preproc', _pop),
'inputnode.t1w_preproc'),
('outputnode.t1w_brain', 'inputnode.t1w_brain'),
('outputnode.t1w_mask', 'inputnode.t1w_mask'),
('outputnode.t1w_dseg', 'inputnode.t1w_dseg'),
('outputnode.t1w_aseg', 'inputnode.t1w_aseg'),
('outputnode.t1w_aparc', 'inputnode.t1w_aparc'),
('outputnode.t1w_tpms', 'inputnode.t1w_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm'),
('outputnode.joint_template', 'inputnode.joint_template'),
('outputnode.joint_anat2std_xfm',
'inputnode.joint_anat2std_xfm'),
('outputnode.joint_std2anat_xfm',
'inputnode.joint_std2anat_xfm'),
# Undefined if --fs-no-reconall, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.t1w2fsnative_xfm',
'inputnode.t1w2fsnative_xfm'),
('outputnode.fsnative2t1w_xfm',
'inputnode.fsnative2t1w_xfm')
]),
])
return workflow
def init_sdc_estimate_wf(fmaps, epi_meta, omp_nthreads=1, debug=False):
"""
Build a :abbr:`SDC (susceptibility distortion correction)` workflow.
This workflow implements the heuristics to choose an estimation
methodology for :abbr:`SDC (susceptibility distortion correction)`.
When no field map information is present within the BIDS inputs,
the EXPERIMENTAL "fieldmap-less SyN" can be performed, using
the ``--use-syn`` argument. When ``--force-syn`` is specified,
then the "fieldmap-less SyN" is always executed and reported
despite of other fieldmaps available with higher priority.
In the latter case (some sort of fieldmap(s) is available and
``--force-syn`` is requested), then the :abbr:`SDC (susceptibility
distortion correction)` method applied is that with the
highest priority.
Parameters
----------
fmaps : list of pybids dicts
A list of dictionaries with the available fieldmaps
(and their metadata using the key ``'metadata'`` for the
case of :abbr:`PEPOLAR (Phase-Encoding POLARity)` fieldmaps).
epi_meta : dict
BIDS metadata dictionary corresponding to the
:abbr:`EPI (echo-planar imaging)` run (i.e., suffix ``bold``,
``sbref``, or ``dwi``) for which the fieldmap is being estimated.
omp_nthreads : int
Maximum number of threads an individual process may use
debug : bool
Enable debugging outputs
Inputs
------
epi_file
A reference image calculated at a previous stage
epi_brain
Same as above, but brain-masked
epi_mask
Brain mask for the run
t1w_brain
T1w image, brain-masked, for the fieldmap-less SyN method
std2anat_xfm
Standard-to-T1w transform generated during spatial
normalization (only for the fieldmap-less SyN method).
Outputs
-------
epi_corrected
The EPI scan reference after unwarping.
epi_mask
The corresponding new mask after unwarping
epi_brain
Brain-extracted, unwarped EPI scan reference
out_warp
The deformation field to unwarp the susceptibility distortions
syn_ref
If ``--force-syn``, an unwarped EPI scan reference with this
method (for reporting purposes)
method : str
Short description of the estimation method that was run.
"""
workflow = Workflow(name='sdc_estimate_wf' if fmaps else 'sdc_bypass_wf')
inputnode = pe.Node(niu.IdentityInterface(fields=[
'epi_file', 'epi_brain', 'epi_mask', 't1w_brain', 'std2anat_xfm'
]),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(fields=[
'epi_corrected', 'epi_mask', 'epi_brain', 'out_warp', 'syn_ref',
'method'
]),
name='outputnode')
# No fieldmaps - forward inputs to outputs
if not fmaps:
workflow.__postdesc__ = """\
Susceptibility distortion correction (SDC) was omitted.
"""
outputnode.inputs.method = 'None'
workflow.connect([
(inputnode, outputnode, [('epi_file', 'epi_corrected'),
('epi_mask', 'epi_mask'),
('epi_brain', 'epi_brain')]),
])
return workflow
workflow.__postdesc__ = """\
Based on the estimated susceptibility distortion, a corrected
EPI (echo-planar imaging) reference was calculated for a more
accurate co-registration with the anatomical reference.
"""
only_syn = 'syn' in fmaps and len(fmaps) == 1
# PEPOLAR path
if 'epi' in fmaps:
from .pepolar import init_pepolar_unwarp_wf, check_pes
# SyN works without this metadata
if epi_meta.get('PhaseEncodingDirection') is None:
raise ValueError(
'PhaseEncodingDirection is not defined within the metadata retrieved '
'for the intended EPI (DWI, BOLD, or SBRef) run.')
outputnode.inputs.method = 'PEB/PEPOLAR (phase-encoding based / PE-POLARity)'
fmaps_epi = [(v[0], v[1].get('PhaseEncodingDirection'))
for v in fmaps['epi']]
if not all(list(zip(*fmaps_epi))[1]):
raise ValueError(
'At least one of the EPI runs with alternative phase-encoding '
'blips is missing the required "PhaseEncodingDirection" metadata entry.'
)
# Find matched PE directions
matched_pe = check_pes(fmaps_epi, epi_meta['PhaseEncodingDirection'])
# Get EPI polarities and their metadata
sdc_unwarp_wf = init_pepolar_unwarp_wf(matched_pe=matched_pe,
omp_nthreads=omp_nthreads)
sdc_unwarp_wf.inputs.inputnode.epi_pe_dir = epi_meta[
'PhaseEncodingDirection']
sdc_unwarp_wf.inputs.inputnode.fmaps_epi = fmaps_epi
workflow.connect([
(inputnode, sdc_unwarp_wf, [('epi_file', 'inputnode.in_reference'),
('epi_brain',
'inputnode.in_reference_brain'),
('epi_mask', 'inputnode.in_mask')]),
])
# FIELDMAP path
elif 'fieldmap' in fmaps or 'phasediff' in fmaps:
from .fmap import init_fmap2field_wf
from .unwarp import init_sdc_unwarp_wf
# SyN works without this metadata
if epi_meta.get('PhaseEncodingDirection') is None:
raise ValueError(
'PhaseEncodingDirection is not defined within the metadata retrieved '
'for the intended EPI (DWI, BOLD, or SBRef) run.')
if 'fieldmap' in fmaps:
from .fmap import init_fmap_wf
try:
fmap, = fmaps['fieldmap']
except ValueError:
LOGGER.warning(
'Several B0 fieldmaps found for the given target, using '
'the first one.')
fmap = fmaps['fieldmap'][0]
outputnode.inputs.method = 'FMB (fieldmap-based) - directly measured B0 map'
fmap_wf = init_fmap_wf(omp_nthreads=omp_nthreads,
fmap_bspline=False)
# set inputs
fmap_wf.inputs.inputnode.magnitude = [
m for m, _ in fmap['magnitude']
]
fmap_wf.inputs.inputnode.fieldmap = [
m for m, _ in fmap['fieldmap']
]
elif 'phasediff' in fmaps:
from .phdiff import init_phdiff_wf
try:
fmap, = fmaps['phasediff']
except ValueError:
LOGGER.warning(
'Several phase-difference maps found for the given target, using '
'the first one.')
fmap = fmaps['phasediff'][0]
outputnode.inputs.method = 'FMB (fieldmap-based) - phase-difference map'
fmap_wf = init_phdiff_wf(omp_nthreads=omp_nthreads)
# set inputs
fmap_wf.inputs.inputnode.magnitude = [
m for m, _ in fmap['magnitude']
]
fmap_wf.inputs.inputnode.phasediff = fmap['phases']
fmap2field_wf = init_fmap2field_wf(omp_nthreads=omp_nthreads,
debug=debug)
fmap2field_wf.inputs.inputnode.metadata = epi_meta
sdc_unwarp_wf = init_sdc_unwarp_wf(omp_nthreads=omp_nthreads,
debug=debug,
name='sdc_unwarp_wf')
workflow.connect([
(inputnode, fmap2field_wf, [('epi_file', 'inputnode.in_reference'),
('epi_brain',
'inputnode.in_reference_brain')]),
(inputnode, sdc_unwarp_wf, [('epi_file', 'inputnode.in_reference'),
('epi_mask',
'inputnode.in_reference_mask')]),
(fmap_wf, fmap2field_wf,
[('outputnode.fmap', 'inputnode.fmap'),
('outputnode.fmap_ref', 'inputnode.fmap_ref'),
('outputnode.fmap_mask', 'inputnode.fmap_mask')]),
(fmap2field_wf, sdc_unwarp_wf, [('outputnode.out_warp',
'inputnode.in_warp')]),
])
elif not only_syn:
raise ValueError('Fieldmaps of types %s are not supported' %
', '.join(['"%s"' % f for f in fmaps]))
# FIELDMAP-less path
if 'syn' in fmaps:
from .syn import init_syn_sdc_wf
syn_sdc_wf = init_syn_sdc_wf(epi_pe=epi_meta.get(
'PhaseEncodingDirection', None),
omp_nthreads=omp_nthreads)
workflow.connect([
(inputnode, syn_sdc_wf,
[('epi_file', 'inputnode.in_reference'),
('epi_brain', 'inputnode.in_reference_brain'),
('t1w_brain', 'inputnode.t1w_brain'),
('std2anat_xfm', 'inputnode.std2anat_xfm')]),
(syn_sdc_wf, outputnode, [('outputnode.out_reference', 'syn_ref')
]),
])
# XXX Eliminate branch when forcing isn't an option
if only_syn: # No fieldmaps, but --use-syn
outputnode.inputs.method = 'FLB ("fieldmap-less", SyN-based)'
sdc_unwarp_wf = syn_sdc_wf
else: # --force-syn was called when other fieldmap was present
sdc_unwarp_wf.__desc__ = None
workflow.connect([
(sdc_unwarp_wf, outputnode,
[('outputnode.out_warp', 'out_warp'),
('outputnode.out_reference', 'epi_corrected'),
('outputnode.out_reference_brain', 'epi_brain'),
('outputnode.out_mask', 'epi_mask')]),
])
return workflow
def init_phdiff_wf(omp_nthreads, debug=False, name="phdiff_wf"):
r"""
Generate a :math:`B_0` field from consecutive-phases and phase-difference maps.
This workflow preprocess phase-difference maps (or generates the phase-difference
map should two ``phase1``/``phase2`` be provided at the input), and generates
an image equivalent to BIDS's ``fieldmap`` that can be processed with the
general fieldmap workflow.
Besides phase2 - phase1 subtraction, the core of this particular workflow relies
in the phase-unwrapping with FSL PRELUDE [Jenkinson2003]_.
FSL PRELUDE takes wrapped maps in the range 0 to 6.28, `as per the user guide
<https://fsl.fmrib.ox.ac.uk/fsl/fslwiki/FUGUE/Guide#Step_2_-_Getting_.28wrapped.29_phase_in_radians>`__.
For the phase-difference maps, recentering back to :math:`[-\pi \dotsb \pi )`
is necessary.
After some massaging and with the scaling of the echo separation factor
:math:`\Delta \text{TE}`, the phase-difference maps are converted into
an actual :math:`B_0` map in Hz units.
Workflow Graph
.. workflow ::
:graph2use: orig
:simple_form: yes
from sdcflows.workflows.fit.fieldmap import init_phdiff_wf
wf = init_phdiff_wf(omp_nthreads=1)
Parameters
----------
omp_nthreads : :obj:`int`
Maximum number of threads an individual process may use
debug : :obj:`bool`
Run on debug mode
name : :obj:`str`
Name of workflow (default: ``phdiff_wf``)
Inputs
------
magnitude : :obj:`os.PathLike`
A reference magnitude image preprocessed elsewhere.
phase : :obj:`list` of :obj:`tuple` of (:obj:`os.PathLike`, :obj:`dict`)
List containing one GRE phase-difference map with its corresponding metadata
(requires ``EchoTime1`` and ``EchoTime2``), or the phase maps for the two
subsequent echoes, with their metadata (requires ``EchoTime``).
mask : :obj:`os.PathLike`
A brain mask calculated from the magnitude image.
Outputs
-------
fieldmap : :obj:`os.PathLike`
The estimated fieldmap in Hz.
"""
from nipype.interfaces.fsl import PRELUDE
from ...interfaces.fmap import Phasediff2Fieldmap, PhaseMap2rads, SubtractPhases
workflow = Workflow(name=name)
workflow.__postdesc__ = f"""\
The corresponding phase-map(s) were phase-unwrapped with `prelude` (FSL {PRELUDE.version}).
"""
inputnode = pe.Node(
niu.IdentityInterface(fields=["magnitude", "phase", "mask"]),
name="inputnode")
outputnode = pe.Node(
niu.IdentityInterface(fields=["fieldmap"]),
name="outputnode",
)
def _split(phase):
return phase
split = pe.MapNode( # We cannot use an inline connection function with MapNode
niu.Function(function=_split, output_names=["map_file", "meta"]),
iterfield=["phase"],
run_without_submitting=True,
name="split",
)
# phase diff -> radians
phmap2rads = pe.MapNode(
PhaseMap2rads(),
name="phmap2rads",
iterfield=["in_file"],
run_without_submitting=True,
)
# FSL PRELUDE will perform phase-unwrapping
prelude = pe.Node(PRELUDE(), name="prelude")
calc_phdiff = pe.Node(SubtractPhases(),
name="calc_phdiff",
run_without_submitting=True)
calc_phdiff.interface._always_run = debug
compfmap = pe.Node(Phasediff2Fieldmap(), name="compfmap")
# fmt: off
workflow.connect([
(inputnode, split, [("phase", "phase")]),
(inputnode, prelude, [("magnitude", "magnitude_file"),
("mask", "mask_file")]),
(split, phmap2rads, [("map_file", "in_file")]),
(phmap2rads, calc_phdiff, [("out_file", "in_phases")]),
(split, calc_phdiff, [("meta", "in_meta")]),
(calc_phdiff, prelude, [("phase_diff", "phase_file")]),
(prelude, compfmap, [("unwrapped_phase_file", "in_file")]),
(calc_phdiff, compfmap, [("metadata", "metadata")]),
(compfmap, outputnode, [("out_file", "fieldmap")]),
])
# fmt: on
return workflow
def init_sdc_wf(boldref, omp_nthreads=1, debug=False, ignore=None):
"""
This workflow implements the heuristics to choose a
:abbr:`SDC (susceptibility distortion correction)` strategy.
When no field map information is present within the BIDS inputs,
the EXPERIMENTAL "fieldmap-less SyN" can be performed, using
the ``--use-syn`` argument. When ``--force-syn`` is specified,
then the "fieldmap-less SyN" is always executed and reported
despite of other fieldmaps available with higher priority.
In the latter case (some sort of fieldmap(s) is available and
``--force-syn`` is requested), then the :abbr:`SDC (susceptibility
distortion correction)` method applied is that with the
highest priority.
.. workflow::
:graph2use: orig
:simple_form: yes
from sdcflows.workflows.base import init_sdc_wf
wf = init_sdc_wf(
fmaps=[{
'suffix': 'phasediff',
'phasediff': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_phasediff.nii.gz',
'magnitude1': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude1.nii.gz',
'magnitude2': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude2.nii.gz',
}],
bold_meta={
'RepetitionTime': 2.0,
'SliceTiming': [0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9],
'PhaseEncodingDirection': 'j',
},
)
**Parameters**
boldref : pybids.BIDSFile
A BIDSFile object with suffix ``bold``, ``sbref`` or ``dwi``.
omp_nthreads : int
Maximum number of threads an individual process may use
debug : bool
Enable debugging outputs
**Inputs**
bold_ref
A BOLD reference calculated at a previous stage
bold_ref_brain
Same as above, but brain-masked
bold_mask
Brain mask for the BOLD run
t1_brain
T1w image, brain-masked, for the fieldmap-less SyN method
std2anat_xfm
List of standard-to-T1w transforms generated during spatial
normalization (only for the fieldmap-less SyN method).
template : str
Name of template from which prior knowledge will be mapped
into the subject's T1w reference
(only for the fieldmap-less SyN method)
templates : str
Name of templates that index the ``std2anat_xfm`` input list
(only for the fieldmap-less SyN method).
**Outputs**
bold_ref
An unwarped BOLD reference
bold_mask
The corresponding new mask after unwarping
bold_ref_brain
Brain-extracted, unwarped BOLD reference
out_warp
The deformation field to unwarp the susceptibility distortions
syn_bold_ref
If ``--force-syn``, an unwarped BOLD reference with this
method (for reporting purposes)
"""
if ignore is None:
ignore = tuple()
if not isinstance(ignore, (list, tuple)):
ignore = tuple(ignore)
fmaps = defaultdict(list, [])
for associated in boldref.get_associations(kind='InformedBy'):
if associated.suffix == 'epi':
fmaps[associated.suffix].append(associated)
# elif associated.suffix in ('phase', 'phasediff', 'fieldmap'):
# fmaps['fieldmap'].append(associated)
workflow = Workflow(name='sdc_wf' if boldref else 'sdc_bypass_wf')
inputnode = pe.Node(niu.IdentityInterface(
fields=['bold_ref', 'bold_ref_brain', 'bold_mask',
't1_brain', 'std2anat_xfm', 'template', 'templates']),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(
fields=['bold_ref', 'bold_mask', 'bold_ref_brain',
'out_warp', 'syn_bold_ref', 'method']),
name='outputnode')
# No fieldmaps - forward inputs to outputs
if not fmaps or 'fieldmaps' in ignore:
workflow.__postdesc__ = """\
Susceptibility distortion correction (SDC) has been skipped because the
dataset does not contain extra field map acquisitions correctly described
with metadata, and the experimental SDC-SyN method was not explicitly selected.
"""
outputnode.inputs.method = 'None'
workflow.connect([
(inputnode, outputnode, [('bold_ref', 'bold_ref'),
('bold_mask', 'bold_mask'),
('bold_ref_brain', 'bold_ref_brain')]),
])
return workflow
workflow.__postdesc__ = """\
Based on the estimated susceptibility distortion, an
unwarped BOLD reference was calculated for a more accurate
co-registration with the anatomical reference.
"""
# PEPOLAR path
if 'epi' in fmaps:
outputnode.inputs.method = 'PEB/PEPOLAR (phase-encoding based / PE-POLARity)'
# Get EPI polarities and their metadata
sdc_unwarp_wf = init_pepolar_unwarp_wf(
bold_meta=boldref.get_metadata(),
epi_fmaps=[(fmap, fmap.get_metadata()["PhaseEncodingDirection"])
for fmap in fmaps['epi']],
omp_nthreads=omp_nthreads,
name='pepolar_unwarp_wf')
workflow.connect([
(inputnode, sdc_unwarp_wf, [
('bold_ref', 'inputnode.in_reference'),
('bold_mask', 'inputnode.in_mask'),
('bold_ref_brain', 'inputnode.in_reference_brain')]),
])
# FIELDMAP path
# elif 'fieldmap' in fmaps:
# # Import specific workflows here, so we don't break everything with one
# # unused workflow.
# suffices = {f.suffix for f in fmaps['fieldmap']}
# if 'fieldmap' in suffices:
# from .fmap import init_fmap_wf
# outputnode.inputs.method = 'FMB (fieldmap-based)'
# fmap_estimator_wf = init_fmap_wf(
# omp_nthreads=omp_nthreads,
# fmap_bspline=False)
# # set inputs
# fmap_estimator_wf.inputs.inputnode.fieldmap = fmap['fieldmap']
# fmap_estimator_wf.inputs.inputnode.magnitude = fmap['magnitude']
# if fmap['suffix'] == 'phasediff':
# from .phdiff import init_phdiff_wf
# fmap_estimator_wf = init_phdiff_wf(omp_nthreads=omp_nthreads)
# # set inputs
# fmap_estimator_wf.inputs.inputnode.phasediff = fmap['phasediff']
# fmap_estimator_wf.inputs.inputnode.magnitude = [
# fmap_ for key, fmap_ in sorted(fmap.items())
# if key.startswith("magnitude")
# ]
# sdc_unwarp_wf = init_sdc_unwarp_wf(
# omp_nthreads=omp_nthreads,
# fmap_demean=fmap_demean,
# debug=debug,
# name='sdc_unwarp_wf')
# sdc_unwarp_wf.inputs.inputnode.metadata = bold_meta
# workflow.connect([
# (inputnode, sdc_unwarp_wf, [
# ('bold_ref', 'inputnode.in_reference'),
# ('bold_ref_brain', 'inputnode.in_reference_brain'),
# ('bold_mask', 'inputnode.in_mask')]),
# (fmap_estimator_wf, sdc_unwarp_wf, [
# ('outputnode.fmap', 'inputnode.fmap'),
# ('outputnode.fmap_ref', 'inputnode.fmap_ref'),
# ('outputnode.fmap_mask', 'inputnode.fmap_mask')]),
# ])
# # FIELDMAP-less path
# if any(fm['suffix'] == 'syn' for fm in fmaps):
# # Select template
# sdc_select_std = pe.Node(KeySelect(
# fields=['std2anat_xfm']),
# name='sdc_select_std', run_without_submitting=True)
# syn_sdc_wf = init_syn_sdc_wf(
# bold_pe=bold_meta.get('PhaseEncodingDirection', None),
# omp_nthreads=omp_nthreads)
# workflow.connect([
# (inputnode, sdc_select_std, [
# ('template', 'key'),
# ('templates', 'keys'),
# ('std2anat_xfm', 'std2anat_xfm')]),
# (sdc_select_std, syn_sdc_wf, [
# ('std2anat_xfm', 'inputnode.std2anat_xfm')]),
# (inputnode, syn_sdc_wf, [
# ('t1_brain', 'inputnode.t1_brain'),
# ('bold_ref', 'inputnode.bold_ref'),
# ('bold_ref_brain', 'inputnode.bold_ref_brain'),
# ('template', 'inputnode.template')]),
# ])
# # XXX Eliminate branch when forcing isn't an option
# if fmap['suffix'] == 'syn': # No fieldmaps, but --use-syn
# outputnode.inputs.method = 'FLB ("fieldmap-less", SyN-based)'
# sdc_unwarp_wf = syn_sdc_wf
# else: # --force-syn was called when other fieldmap was present
# sdc_unwarp_wf.__desc__ = None
# workflow.connect([
# (syn_sdc_wf, outputnode, [
# ('outputnode.out_reference', 'syn_bold_ref')]),
# ])
workflow.connect([
(sdc_unwarp_wf, outputnode, [
('outputnode.out_warp', 'out_warp'),
('outputnode.out_reference', 'bold_ref'),
('outputnode.out_reference_brain', 'bold_ref_brain'),
('outputnode.out_mask', 'bold_mask')]),
])
return workflow
def init_single_subject_wf(subject_id):
"""
Set-up the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and diffusion MRI preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Diffusion MRI preprocessing is performed using a separate workflow for
a full :abbr:`DWI (diffusion weighted imaging)` *entity*.
A DWI *entity* may comprehend one or several runs (for instance, two
opposed :abbr:`PE (phase-encoding)` directions.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from dmriprep.config.testing import mock_config
from dmriprep.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf('THP0005')
Parameters
----------
subject_id : str
List of subject labels
Inputs
------
subjects_dir : os.pathlike
FreeSurfer's ``$SUBJECTS_DIR``
"""
name = "single_subject_%s_wf" % subject_id
subject_data = collect_data(config.execution.layout, subject_id)[0]
if 'flair' in config.workflow.ignore:
subject_data['flair'] = []
if 't2w' in config.workflow.ignore:
subject_data['t2w'] = []
anat_only = config.workflow.anat_only
# Make sure we always go through these two checks
if not anat_only and not subject_data['dwi']:
raise Exception(f"No DWI data found for participant {subject_id}. "
"All workflows require DWI images.")
if not subject_data['t1w']:
raise Exception(f"No T1w images found for participant {subject_id}. "
"All workflows require T1w images.")
workflow = Workflow(name=name)
workflow.__desc__ = f"""
Results included in this manuscript come from preprocessing
performed using *dMRIPrep* {config.environment.version}
(@dmriprep; RRID:SCR_017412),
which is based on *Nipype* {config.environment.nipype_version}
(@nipype1; @nipype2; RRID:SCR_002502).
"""
workflow.__postdesc__ = """
For more details of the pipeline, see [the section corresponding
to workflows in *dMRIPrep*'s documentation]\
(https://nipreps.github.io/dmriprep/master/workflows.html \
"dMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by dMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
"""
spaces = config.workflow.spaces
reportlets_dir = str(config.execution.work_dir / 'reportlets')
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(bids_dir=config.execution.bids_dir,
bids_validate=False),
name='bids_info')
summary = pe.Node(SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False)),
name='summary',
run_without_submitting=True)
about = pe.Node(AboutSummary(version=config.environment.version,
command=' '.join(sys.argv)),
name='about',
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='summary', keep_dtype=True),
name='ds_report_summary',
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='about', keep_dtype=True),
name='ds_report_about',
run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=str(config.execution.bids_dir),
debug=config.execution.debug is True,
freesurfer=config.workflow.run_reconall,
hires=config.workflow.hires,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=str(config.execution.output_dir),
reportlets_dir=reportlets_dir,
skull_strip_fixed_seed=config.workflow.skull_strip_fixed_seed,
skull_strip_mode='force',
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
spaces=spaces,
t1w=subject_data['t1w'],
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')
]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w'), ('dwi', 'dwi')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix),
'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_'):
workflow.get_node(node).interface.out_path_base = 'dmriprep'
if anat_only:
return workflow
# Append the dMRI section to the existing anatomical excerpt
# That way we do not need to stream down the number of bold datasets
anat_preproc_wf.__postdesc__ = (anat_preproc_wf.__postdesc__ or '') + f"""
Diffusion data preprocessing
: For each of the {len(subject_data["dwi"])} dwi scans found per subject
(across all sessions), the following preprocessing was performed."""
for dwi_file in subject_data['dwi']:
dwi_preproc_wf = init_dwi_preproc_wf(dwi_file)
workflow.connect([
(
anat_preproc_wf,
dwi_preproc_wf,
[
(('outputnode.t1w_preproc', _pop),
'inputnode.t1w_preproc'),
('outputnode.t1w_mask', 'inputnode.t1w_mask'),
('outputnode.t1w_dseg', 'inputnode.t1w_dseg'),
('outputnode.t1w_aseg', 'inputnode.t1w_aseg'),
('outputnode.t1w_aparc', 'inputnode.t1w_aparc'),
('outputnode.t1w_tpms', 'inputnode.t1w_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm'),
# Undefined if --fs-no-reconall, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.t1w2fsnative_xfm',
'inputnode.t1w2fsnative_xfm'),
('outputnode.fsnative2t1w_xfm',
'inputnode.fsnative2t1w_xfm')
]),
])
return workflow
def init_fmap_wf(omp_nthreads=1,
debug=False,
mode="phasediff",
name="fmap_wf"):
"""
Estimate the fieldmap based on a field-mapping MRI acquisition.
Estimates the fieldmap using either one phase-difference map or
image and one or more
magnitude images corresponding to two or more :abbr:`GRE (Gradient Echo sequence)`
acquisitions.
When we have a sequence that directly measures the fieldmap,
we just need to mask it (using the corresponding magnitude image)
to remove the noise in the surrounding air region, and ensure that
units are Hz.
Workflow Graph
.. workflow ::
:graph2use: orig
:simple_form: yes
from sdcflows.workflows.fit.fieldmap import init_fmap_wf
wf = init_fmap_wf(omp_nthreads=6)
Parameters
----------
omp_nthreads : :obj:`int`
Maximum number of threads an individual process may use.
debug : :obj:`bool`
Run on debug mode
name : :obj:`str`
Unique name of this workflow.
Inputs
------
magnitude : :obj:`list` of :obj:`str`
Path to the corresponding magnitude image for anatomical reference.
fieldmap : :obj:`list` of :obj:`tuple`(:obj:`str`, :obj:`dict`)
Path to the fieldmap acquisition (``*_fieldmap.nii[.gz]`` of BIDS).
Outputs
-------
fmap : :obj:`str`
Path to the estimated fieldmap.
fmap_ref : :obj:`str`
Path to a preprocessed magnitude image reference.
fmap_coeff : :obj:`str` or :obj:`list` of :obj:`str`
The path(s) of the B-Spline coefficients supporting the fieldmap.
fmap_mask : :obj:`str`
Path to a binary brain mask corresponding to the ``fmap`` and ``fmap_ref``
pair.
"""
from ...interfaces.bspline import (
BSplineApprox,
DEFAULT_LF_ZOOMS_MM,
DEFAULT_HF_ZOOMS_MM,
DEFAULT_ZOOMS_MM,
)
workflow = Workflow(name=name)
inputnode = pe.Node(
niu.IdentityInterface(fields=["magnitude", "fieldmap"]),
name="inputnode")
outputnode = pe.Node(
niu.IdentityInterface(
fields=["fmap", "fmap_ref", "fmap_mask", "fmap_coeff"]),
name="outputnode",
)
magnitude_wf = init_magnitude_wf(omp_nthreads=omp_nthreads)
bs_filter = pe.Node(BSplineApprox(),
n_procs=omp_nthreads,
name="bs_filter")
bs_filter.interface._always_run = debug
bs_filter.inputs.bs_spacing = ([DEFAULT_LF_ZOOMS_MM, DEFAULT_HF_ZOOMS_MM]
if not debug else [DEFAULT_ZOOMS_MM])
bs_filter.inputs.extrapolate = not debug
# fmt: off
workflow.connect([
(inputnode, magnitude_wf, [("magnitude", "inputnode.magnitude")]),
(magnitude_wf, bs_filter, [("outputnode.fmap_mask", "in_mask")]),
(magnitude_wf, outputnode, [
("outputnode.fmap_mask", "fmap_mask"),
("outputnode.fmap_ref", "fmap_ref"),
]),
(bs_filter, outputnode,
[("out_extrapolated" if not debug else "out_field", "fmap"),
("out_coeff", "fmap_coeff")]),
])
# fmt: on
if mode == "phasediff":
workflow.__postdesc__ = """\
A *B<sub>0</sub>* nonuniformity map (or *fieldmap*) was estimated from the
phase-drift map(s) measure with two consecutive GRE (gradient-recalled echo)
acquisitions.
"""
phdiff_wf = init_phdiff_wf(omp_nthreads, debug=debug)
# fmt: off
workflow.connect([
(inputnode, phdiff_wf, [("fieldmap", "inputnode.phase")]),
(magnitude_wf, phdiff_wf, [
("outputnode.fmap_ref", "inputnode.magnitude"),
("outputnode.fmap_mask", "inputnode.mask"),
]),
(phdiff_wf, bs_filter, [
("outputnode.fieldmap", "in_data"),
]),
])
# fmt: on
else:
from niworkflows.interfaces.images import IntraModalMerge
from ...interfaces.fmap import CheckB0Units
workflow.__postdesc__ = """\
A *B<sub>0</sub>* nonuniformity map (or *fieldmap*) was directly measured with
an MRI scheme designed with that purpose such as SEI (Spiral-Echo Imaging).
"""
# Merge input fieldmap images (assumes all are given in the same units!)
fmapmrg = pe.Node(
IntraModalMerge(zero_based_avg=False, hmc=False, to_ras=False),
name="fmapmrg",
)
units = pe.Node(CheckB0Units(),
name="units",
run_without_submitting=True)
# fmt: off
workflow.connect([
(inputnode, units, [(("fieldmap", _get_units), "units")]),
(inputnode, fmapmrg, [(("fieldmap", _get_file), "in_files")]),
(fmapmrg, units, [("out_avg", "in_file")]),
(units, bs_filter, [("out_file", "in_data")]),
])
# fmt: on
return workflow
def init_func_preproc_wf(bold_file):
"""
This workflow controls the functional preprocessing stages of *fMRIPrep*.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from fprodents.workflows.tests import mock_config
from fprodents import config
from fprodents.workflows.bold.base import init_func_preproc_wf
with mock_config():
bold_file = config.execution.bids_dir / 'sub-01' / 'func' \
/ 'sub-01_task-mixedgamblestask_run-01_bold.nii.gz'
wf = init_func_preproc_wf(str(bold_file))
Inputs
------
bold_file
BOLD series NIfTI file
t1w_preproc
Bias-corrected structural template image
t1w_mask
Mask of the skull-stripped template image
anat_dseg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1w_asec
Segmentation of structural image, done with FreeSurfer.
t1w_aparc
Parcellation of structural image, done with FreeSurfer.
anat_tpms
List of tissue probability maps in T1w space
template
List of templates to target
anat2std_xfm
List of transform files, collated with templates
std2anat_xfm
List of inverse transform files, collated with templates
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1w2fsnative_xfm
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
fsnative2t1w_xfm
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
Outputs
-------
bold_t1
BOLD series, resampled to T1w space
bold_mask_t1
BOLD series mask in T1w space
bold_std
BOLD series, resampled to template space
bold_mask_std
BOLD series mask in template space
confounds
TSV of confounds
surfaces
BOLD series, resampled to FreeSurfer surfaces
aroma_noise_ics
Noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
See Also
--------
* :py:func:`~fprodents.workflows.bold.stc.init_bold_stc_wf`
* :py:func:`~fprodents.workflows.bold.hmc.init_bold_hmc_wf`
* :py:func:`~fprodents.workflows.bold.t2s.init_bold_t2s_wf`
* :py:func:`~fprodents.workflows.bold.registration.init_bold_t1_trans_wf`
* :py:func:`~fprodents.workflows.bold.registration.init_bold_reg_wf`
* :py:func:`~fprodents.workflows.bold.confounds.init_bold_confounds_wf`
* :py:func:`~fprodents.workflows.bold.confounds.init_ica_aroma_wf`
* :py:func:`~fprodents.workflows.bold.resampling.init_bold_std_trans_wf`
* :py:func:`~fprodents.workflows.bold.resampling.init_bold_preproc_trans_wf`
* :py:func:`~fprodents.workflows.bold.resampling.init_bold_surf_wf`
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.fixes import FixHeaderApplyTransforms as ApplyTransforms
from niworkflows.interfaces.nibabel import ApplyMask
from niworkflows.interfaces.utility import KeySelect, DictMerge
from nipype.interfaces.freesurfer.utils import LTAConvert
from ...patch.utils import extract_entities
mem_gb = {"filesize": 1, "resampled": 1, "largemem": 1}
bold_tlen = 10
# Have some options handy
omp_nthreads = config.nipype.omp_nthreads
spaces = config.workflow.spaces
output_dir = str(config.execution.output_dir)
# Extract BIDS entities and metadata from BOLD file(s)
entities = extract_entities(bold_file)
layout = config.execution.layout
# Take first file as reference
ref_file = pop_file(bold_file)
metadata = layout.get_metadata(ref_file)
echo_idxs = listify(entities.get("echo", []))
multiecho = len(echo_idxs) > 2
if len(echo_idxs) == 1:
config.loggers.warning(
f"Running a single echo <{ref_file}> from a seemingly multi-echo dataset."
)
bold_file = ref_file # Just in case - drop the list
if len(echo_idxs) == 2:
raise RuntimeError(
"Multi-echo processing requires at least three different echos (found two)."
)
if multiecho:
# Drop echo entity for future queries, have a boolean shorthand
entities.pop("echo", None)
# reorder echoes from shortest to largest
tes, bold_file = zip(*sorted([(layout.get_metadata(bf)["EchoTime"], bf)
for bf in bold_file]))
ref_file = bold_file[0] # Reset reference to be the shortest TE
if os.path.isfile(ref_file):
bold_tlen, mem_gb = _create_mem_gb(ref_file)
wf_name = _get_wf_name(ref_file)
config.loggers.workflow.debug(
"Creating bold processing workflow for <%s> (%.2f GB / %d TRs). "
"Memory resampled/largemem=%.2f/%.2f GB.",
ref_file,
mem_gb["filesize"],
bold_tlen,
mem_gb["resampled"],
mem_gb["largemem"],
)
# Find associated sbref, if possible
entities["suffix"] = "sbref"
entities["extension"] = ["nii", "nii.gz"] # Overwrite extensions
sbref_files = layout.get(return_type="file", **entities)
sbref_msg = f"No single-band-reference found for {os.path.basename(ref_file)}."
if sbref_files and "sbref" in config.workflow.ignore:
sbref_msg = "Single-band reference file(s) found and ignored."
elif sbref_files:
sbref_msg = "Using single-band reference file(s) {}.".format(",".join(
[os.path.basename(sbf) for sbf in sbref_files]))
config.loggers.workflow.info(sbref_msg)
# Check whether STC must/can be run
run_stc = (bool(metadata.get("SliceTiming"))
and 'slicetiming' not in config.workflow.ignore)
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__postdesc__ = """\
All resamplings can be performed with *a single interpolation
step* by composing all the pertinent transformations (i.e. head-motion
transform matrices, susceptibility distortion correction when available,
and co-registrations to anatomical and output spaces).
Gridded (volumetric) resamplings were performed using `antsApplyTransforms` (ANTs),
configured with Lanczos interpolation to minimize the smoothing
effects of other kernels [@lanczos].
Non-gridded (surface) resamplings were performed using `mri_vol2surf`
(FreeSurfer).
"""
inputnode = pe.Node(
niu.IdentityInterface(fields=[
"bold_file",
"ref_file",
"bold_ref_xfm",
"n_dummy_scans",
"validation_report",
"subjects_dir",
"subject_id",
"anat_preproc",
"anat_mask",
"anat_dseg",
"anat_tpms",
"anat2std_xfm",
"std2anat_xfm",
"template",
"anat2fsnative_xfm",
"fsnative2anat_xfm",
]),
name="inputnode",
)
inputnode.inputs.bold_file = bold_file
outputnode = pe.Node(
niu.IdentityInterface(fields=[
"bold_mask",
"bold_t1",
"bold_t1_ref",
"bold_mask_t1",
"bold_std",
"bold_std_ref",
"bold_mask_std",
"bold_native",
"surfaces",
"confounds",
"aroma_noise_ics",
"melodic_mix",
"nonaggr_denoised_file",
"confounds_metadata",
]),
name="outputnode",
)
# Generate a brain-masked conversion of the t1w and bold reference images
t1w_brain = pe.Node(ApplyMask(), name="t1w_brain")
lta_convert = pe.Node(LTAConvert(out_fsl=True, out_itk=True),
name="lta_convert")
# BOLD buffer: an identity used as a pointer to either the original BOLD
# or the STC'ed one for further use.
boldbuffer = pe.Node(niu.IdentityInterface(fields=["bold_file"]),
name="boldbuffer")
summary = pe.Node(
FunctionalSummary(
slice_timing=run_stc,
registration="FSL",
registration_dof=config.workflow.bold2t1w_dof,
registration_init=config.workflow.bold2t1w_init,
pe_direction=metadata.get("PhaseEncodingDirection"),
echo_idx=echo_idxs,
tr=metadata.get("RepetitionTime"),
distortion_correction="<not implemented>",
),
name="summary",
mem_gb=config.DEFAULT_MEMORY_MIN_GB,
run_without_submitting=True,
)
summary.inputs.dummy_scans = config.workflow.dummy_scans
func_derivatives_wf = init_func_derivatives_wf(
bids_root=layout.root,
metadata=metadata,
output_dir=output_dir,
spaces=spaces,
use_aroma=config.workflow.use_aroma,
)
# fmt:off
workflow.connect([
(outputnode, func_derivatives_wf, [
('bold_t1', 'inputnode.bold_t1'),
('bold_t1_ref', 'inputnode.bold_t1_ref'),
('bold_mask_t1', 'inputnode.bold_mask_t1'),
('bold_native', 'inputnode.bold_native'),
('confounds', 'inputnode.confounds'),
('surfaces', 'inputnode.surf_files'),
('aroma_noise_ics', 'inputnode.aroma_noise_ics'),
('melodic_mix', 'inputnode.melodic_mix'),
('nonaggr_denoised_file', 'inputnode.nonaggr_denoised_file'),
('confounds_metadata', 'inputnode.confounds_metadata'),
]),
])
# fmt:on
# Top-level BOLD splitter
bold_split = pe.Node(FSLSplit(dimension="t"),
name="bold_split",
mem_gb=mem_gb["filesize"] * 3)
# calculate BOLD registration to T1w
bold_reg_wf = init_bold_reg_wf(
bold2t1w_dof=config.workflow.bold2t1w_dof,
bold2t1w_init=config.workflow.bold2t1w_init,
mem_gb=mem_gb["resampled"],
name="bold_reg_wf",
omp_nthreads=omp_nthreads,
use_compression=False,
)
# apply BOLD registration to T1w
bold_t1_trans_wf = init_bold_t1_trans_wf(
name="bold_t1_trans_wf",
use_fieldwarp=False,
multiecho=multiecho,
mem_gb=mem_gb["resampled"],
omp_nthreads=omp_nthreads,
use_compression=False,
)
t1w_mask_bold_tfm = pe.Node(ApplyTransforms(interpolation="MultiLabel"),
name="t1w_mask_bold_tfm",
mem_gb=0.1)
# get confounds
bold_confounds_wf = init_bold_confs_wf(
mem_gb=mem_gb["largemem"],
metadata=metadata,
regressors_all_comps=config.workflow.regressors_all_comps,
regressors_fd_th=config.workflow.regressors_fd_th,
regressors_dvars_th=config.workflow.regressors_dvars_th,
name="bold_confounds_wf",
)
bold_confounds_wf.get_node("inputnode").inputs.t1_transform_flags = [False]
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_bold_trans_wf = init_bold_preproc_trans_wf(
mem_gb=mem_gb["resampled"],
omp_nthreads=omp_nthreads,
use_compression=not config.execution.low_mem,
use_fieldwarp=False,
name="bold_bold_trans_wf",
)
bold_bold_trans_wf.inputs.inputnode.name_source = ref_file
# SLICE-TIME CORRECTION (or bypass) #############################################
if run_stc:
bold_stc_wf = init_bold_stc_wf(name="bold_stc_wf", metadata=metadata)
# fmt:off
workflow.connect([
(inputnode, bold_stc_wf, [("n_dummy_scans", "inputnode.skip_vols")
]),
(bold_stc_wf, boldbuffer, [("outputnode.stc_file", "bold_file")]),
])
# fmt:on
if not multiecho:
# fmt:off
workflow.connect([(inputnode, bold_stc_wf,
[('bold_file', 'inputnode.bold_file')])])
# fmt:on
else: # for meepi, iterate through stc_wf for all workflows
meepi_echos = boldbuffer.clone(name="meepi_echos")
meepi_echos.iterables = ("bold_file", bold_file)
# fmt:off
workflow.connect([(meepi_echos, bold_stc_wf,
[('bold_file', 'inputnode.bold_file')])])
# fmt:on
elif not multiecho: # STC is too short or False
# bypass STC from original BOLD to the splitter through boldbuffer
# fmt:off
workflow.connect([(inputnode, boldbuffer, [('bold_file', 'bold_file')])
])
# fmt:on
else:
# for meepi, iterate over all meepi echos to boldbuffer
boldbuffer.iterables = ("bold_file", bold_file)
# MULTI-ECHO EPI DATA #############################################
if multiecho:
from niworkflows.func.util import init_skullstrip_bold_wf
skullstrip_bold_wf = init_skullstrip_bold_wf(name="skullstrip_bold_wf")
inputnode.inputs.bold_file = ref_file # Replace reference w first echo
join_echos = pe.JoinNode(
niu.IdentityInterface(fields=["bold_files"]),
joinsource=("meepi_echos" if run_stc is True else "boldbuffer"),
joinfield=["bold_files"],
name="join_echos",
)
# create optimal combination, adaptive T2* map
bold_t2s_wf = init_bold_t2s_wf(
echo_times=tes,
mem_gb=mem_gb["resampled"],
omp_nthreads=omp_nthreads,
name="bold_t2smap_wf",
)
# fmt:off
workflow.connect([
(skullstrip_bold_wf, join_echos,
[('outputnode.skull_stripped_file', 'bold_files')]),
(join_echos, bold_t2s_wf, [('bold_files', 'inputnode.bold_file')]),
])
# fmt:on
# MAIN WORKFLOW STRUCTURE #######################################################
# fmt:off
workflow.connect([
(inputnode, bold_reg_wf, [('anat_preproc', 'inputnode.t1w_brain'),
('ref_file', 'inputnode.ref_bold_brain')]),
(inputnode, t1w_brain, [('anat_preproc', 'in_file'),
('anat_mask', 'in_mask')]),
# convert bold reference LTA transform to other formats
(inputnode, lta_convert, [('bold_ref_xfm', 'in_lta')]),
# BOLD buffer has slice-time corrected if it was run, original otherwise
(boldbuffer, bold_split, [('bold_file', 'in_file')]),
(inputnode, summary, [('n_dummy_scans', 'algo_dummy_scans')]),
# EPI-T1 registration workflow
(inputnode, bold_t1_trans_wf, [('bold_file', 'inputnode.name_source'),
('anat_mask', 'inputnode.t1w_mask'),
('ref_file', 'inputnode.ref_bold_brain')
]),
(t1w_brain, bold_t1_trans_wf, [('out_file', 'inputnode.t1w_brain')]),
(lta_convert, bold_t1_trans_wf, [('out_itk', 'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_t1_trans_wf, [('outputnode.bold2anat',
'inputnode.bold2anat')]),
(bold_t1_trans_wf, outputnode, [('outputnode.bold_t1', 'bold_t1'),
('outputnode.bold_t1_ref',
'bold_t1_ref')]),
# transform T1 mask to BOLD
(inputnode, t1w_mask_bold_tfm, [('anat_mask', 'input_image'),
('ref_file', 'reference_image')]),
(bold_reg_wf, t1w_mask_bold_tfm, [('outputnode.anat2bold',
'transforms')]),
(t1w_mask_bold_tfm, outputnode, [('output_image', 'bold_mask')]),
# Connect bold_confounds_wf
(inputnode, bold_confounds_wf, [('anat_tpms', 'inputnode.anat_tpms'),
('anat_mask', 'inputnode.t1w_mask')]),
(lta_convert, bold_confounds_wf, [('out_fsl', 'inputnode.movpar_file')
]),
(bold_reg_wf, bold_confounds_wf, [('outputnode.anat2bold',
'inputnode.anat2bold')]),
(inputnode, bold_confounds_wf, [('n_dummy_scans',
'inputnode.skip_vols')]),
(t1w_mask_bold_tfm, bold_confounds_wf, [('output_image',
'inputnode.bold_mask')]),
(bold_confounds_wf, outputnode, [('outputnode.confounds_file',
'confounds')]),
(bold_confounds_wf, outputnode, [('outputnode.confounds_metadata',
'confounds_metadata')]),
# Connect bold_bold_trans_wf
(inputnode, bold_bold_trans_wf, [('ref_file', 'inputnode.bold_ref')]),
(t1w_mask_bold_tfm, bold_bold_trans_wf, [('output_image',
'inputnode.bold_mask')]),
(bold_split, bold_bold_trans_wf, [('out_files', 'inputnode.bold_file')
]),
(lta_convert, bold_bold_trans_wf, [('out_itk', 'inputnode.hmc_xforms')
]),
# Summary
(outputnode, summary, [('confounds', 'confounds_file')]),
])
# fmt:on
# for standard EPI data, pass along correct file
if not multiecho:
# fmt:off
workflow.connect([
(inputnode, func_derivatives_wf, [('bold_file',
'inputnode.source_file')]),
(bold_bold_trans_wf, bold_confounds_wf, [('outputnode.bold',
'inputnode.bold')]),
(bold_split, bold_t1_trans_wf, [('out_files',
'inputnode.bold_split')]),
])
# fmt:on
else: # for meepi, create and use optimal combination
# fmt:off
workflow.connect([
# update name source for optimal combination
(inputnode, func_derivatives_wf,
[(('bold_file', combine_meepi_source), 'inputnode.source_file')]),
(bold_bold_trans_wf, skullstrip_bold_wf, [('outputnode.bold',
'inputnode.in_file')]),
(bold_t2s_wf, bold_confounds_wf, [('outputnode.bold',
'inputnode.bold')]),
(bold_t2s_wf, bold_t1_trans_wf, [('outputnode.bold',
'inputnode.bold_split')]),
])
# fmt:on
# Map final BOLD mask into T1w space (if required)
nonstd_spaces = set(spaces.get_nonstandard())
if nonstd_spaces.intersection(("T1w", "anat")):
from niworkflows.interfaces.fixes import (
FixHeaderApplyTransforms as ApplyTransforms, )
boldmask_to_t1w = pe.Node(
ApplyTransforms(interpolation="MultiLabel"),
name="boldmask_to_t1w",
mem_gb=0.1,
)
# fmt:off
workflow.connect([
(bold_reg_wf, boldmask_to_t1w, [('outputnode.bold2anat',
'transforms')]),
(bold_t1_trans_wf, boldmask_to_t1w, [('outputnode.bold_mask_t1',
'reference_image')]),
(t1w_mask_bold_tfm, boldmask_to_t1w, [('output_image',
'input_image')]),
(boldmask_to_t1w, outputnode, [('output_image', 'bold_mask_t1')]),
])
# fmt:on
if nonstd_spaces.intersection(("func", "run", "bold", "boldref", "sbref")):
# fmt:off
workflow.connect([
(bold_bold_trans_wf, outputnode, [('outputnode.bold',
'bold_native')]),
(bold_bold_trans_wf, func_derivatives_wf,
[('outputnode.bold_ref', 'inputnode.bold_native_ref'),
('outputnode.bold_mask', 'inputnode.bold_mask_native')]),
])
# fmt:on
if spaces.get_spaces(nonstandard=False, dim=(3, )):
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_std_trans_wf = init_bold_std_trans_wf(
mem_gb=mem_gb["resampled"],
omp_nthreads=omp_nthreads,
spaces=spaces,
name="bold_std_trans_wf",
use_compression=not config.execution.low_mem,
use_fieldwarp=False,
)
# fmt:off
workflow.connect([
(inputnode, bold_std_trans_wf,
[('template', 'inputnode.templates'),
('anat2std_xfm', 'inputnode.anat2std_xfm'),
('bold_file', 'inputnode.name_source')]),
(t1w_mask_bold_tfm, bold_std_trans_wf, [('output_image',
'inputnode.bold_mask')]),
(lta_convert, bold_std_trans_wf, [('out_itk',
'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_std_trans_wf, [('outputnode.bold2anat',
'inputnode.bold2anat')]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_std', 'bold_std'),
('outputnode.bold_std_ref', 'bold_std_ref'),
('outputnode.bold_mask_std', 'bold_mask_std')]),
])
# fmt:on
if not multiecho:
# fmt:off
workflow.connect([
(bold_split, bold_std_trans_wf, [("out_files",
"inputnode.bold_split")]),
])
# fmt:on
else:
split_opt_comb = bold_split.clone(name="split_opt_comb")
# fmt:off
workflow.connect([(bold_t2s_wf, split_opt_comb,
[('outputnode.bold', 'in_file')]),
(split_opt_comb, bold_std_trans_wf,
[('out_files', 'inputnode.bold_split')])])
# fmt:on
# func_derivatives_wf internally parametrizes over snapshotted spaces.
# fmt:off
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('outputnode.template', 'inputnode.template'),
('outputnode.spatial_reference',
'inputnode.spatial_reference'),
('outputnode.bold_std_ref', 'inputnode.bold_std_ref'),
('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
]),
])
# fmt:on
if config.workflow.use_aroma: # ICA-AROMA workflow
from .confounds import init_ica_aroma_wf
ica_aroma_wf = init_ica_aroma_wf(
mem_gb=mem_gb["resampled"],
metadata=metadata,
omp_nthreads=omp_nthreads,
use_fieldwarp=False,
err_on_aroma_warn=config.workflow.aroma_err_on_warn,
aroma_melodic_dim=config.workflow.aroma_melodic_dim,
name="ica_aroma_wf",
)
join = pe.Node(
niu.Function(output_names=["out_file"], function=_to_join),
name="aroma_confounds",
)
mrg_conf_metadata = pe.Node(
niu.Merge(2),
name="merge_confound_metadata",
run_without_submitting=True,
)
mrg_conf_metadata2 = pe.Node(
DictMerge(),
name="merge_confound_metadata2",
run_without_submitting=True,
)
# fmt:off
workflow.disconnect([
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_metadata', 'confounds_metadata'),
]),
])
workflow.connect([
(inputnode, ica_aroma_wf,
[('bold_file', 'inputnode.name_source'),
('n_dummy_scans', 'inputnode.skip_vols')]),
(lta_convert, ica_aroma_wf, [('out_fsl',
'inputnode.movpar_file')]),
(bold_confounds_wf, join, [('outputnode.confounds_file',
'in_file')]),
(bold_confounds_wf, mrg_conf_metadata,
[('outputnode.confounds_metadata', 'in1')]),
(ica_aroma_wf, join, [('outputnode.aroma_confounds',
'join_file')]),
(ica_aroma_wf, mrg_conf_metadata,
[('outputnode.aroma_metadata', 'in2')]),
(mrg_conf_metadata, mrg_conf_metadata2, [('out', 'in_dicts')]),
(ica_aroma_wf, outputnode,
[('outputnode.aroma_noise_ics', 'aroma_noise_ics'),
('outputnode.melodic_mix', 'melodic_mix'),
('outputnode.nonaggr_denoised_file', 'nonaggr_denoised_file')
]),
(join, outputnode, [('out_file', 'confounds')]),
(mrg_conf_metadata2, outputnode, [('out_dict',
'confounds_metadata')]),
(bold_std_trans_wf, ica_aroma_wf,
[('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
('outputnode.spatial_reference',
'inputnode.spatial_reference')]),
])
# fmt:on
if spaces.get_spaces(nonstandard=False, dim=(3, )):
carpetplot_wf = init_carpetplot_wf(
mem_gb=mem_gb["resampled"],
metadata=metadata,
name="carpetplot_wf",
)
# Xform to 'Fischer344' is always computed.
carpetplot_select_std = pe.Node(
KeySelect(fields=["std2anat_xfm"], key="Fischer344"),
name="carpetplot_select_std",
run_without_submitting=True,
)
# fmt:off
workflow.connect([
(inputnode, carpetplot_select_std, [('std2anat_xfm',
'std2anat_xfm'),
('template', 'keys')]),
(carpetplot_select_std, carpetplot_wf,
[('std2anat_xfm', 'inputnode.std2anat_xfm')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf,
carpetplot_wf, [('outputnode.bold', 'inputnode.bold')]),
(t1w_mask_bold_tfm, carpetplot_wf, [('output_image',
'inputnode.bold_mask')]),
(bold_reg_wf, carpetplot_wf, [('outputnode.anat2bold',
'inputnode.anat2bold')]),
(bold_confounds_wf, carpetplot_wf, [('outputnode.confounds_file',
'inputnode.confounds_file')]),
])
# fmt:on
# REPORTING ############################################################
ds_report_summary = pe.Node(
DerivativesDataSink(desc="summary",
datatype="figures",
dismiss_entities=("echo", )),
name="ds_report_summary",
run_without_submitting=True,
mem_gb=config.DEFAULT_MEMORY_MIN_GB,
)
ds_report_validation = pe.Node(
DerivativesDataSink(
base_directory=output_dir,
desc="validation",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_validation",
run_without_submitting=True,
mem_gb=config.DEFAULT_MEMORY_MIN_GB,
)
# fmt:off
workflow.connect([
(summary, ds_report_summary, [('out_report', 'in_file')]),
(inputnode, ds_report_validation, [('validation_report', 'in_file')]),
])
# fmt:on
# Fill-in datasinks of reportlets seen so far
for node in workflow.list_node_names():
if node.split(".")[-1].startswith("ds_report"):
workflow.get_node(node).inputs.base_directory = output_dir
workflow.get_node(node).inputs.source_file = ref_file
return workflow
def init_dwi_preproc_wf(
dwi_file,
debug,
force_syn,
ignore,
low_mem,
omp_nthreads,
output_dir,
reportlets_dir,
use_syn,
layout=None,
num_dwi=1,
):
"""
This workflow controls the diffusion preprocessing stages of *dMRIPrep*.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from dmriprep.workflows.dwi import init_dwi_preproc_wf
from collections import namedtuple
BIDSLayout = namedtuple('BIDSLayout', ['root'])
wf = init_dwi_preproc_wf(
dwi_file='/completely/made/up/path/sub-01_dwi.nii.gz',
debug=False,
force_syn=True,
ignore=[],
low_mem=False,
omp_nthreads=1,
output_dir='.',
reportlets_dir='.',
use_syn=True,
layout=BIDSLayout('.'),
num_dwi=1,
)
Parameters
----------
dwi_file : str
dwi NIfTI file
debug : bool
Enable debugging outputs
force_syn : bool
**Temporary**: Always run SyN-based SDC
ignore : list
Preprocessing steps to skip (may include "sdc")
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
omp_nthreads : int
Maximum number of threads an individual process may use
output_dir : str
Directory in which to save derivatives
reportlets_dir : str
Absolute path of a directory in which reportlets will be temporarily stored
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
layout : BIDSLayout
BIDSLayout structure to enable metadata retrieval
num_dwi : int
Total number of dwi files that have been set for preprocessing
(default is 1)
Inputs
------
dwi_file
dwi NIfTI file
bvec_file
File path of the b-values
bval_file
File path of the b-vectors
Outputs
-------
dwi_file
dwi NIfTI file
dwi_mask
dwi mask
See also
--------
* :py:func:`~dmriprep.workflows.dwi.util.init_dwi_reference_wf`
* :py:func:`~dmriprep.workflows.dwi.outputs.init_reportlets_wf`
"""
wf_name = _get_wf_name(dwi_file)
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__desc__ = """
Diffusion data preprocessing
: For each of the {num_dwi} dwi scans found per subject (across all sessions),
the following preprocessing was performed.
""".format(num_dwi=num_dwi)
workflow.__postdesc__ = """\
"""
# For doc building purposes
if not hasattr(layout, 'parse_file_entities'):
LOGGER.log(25, 'No valid layout: building empty workflow.')
bvec_file = '/completely/made/up/path/sub-01_dwi.bvec'
bval_file = '/completely/made/up/path/sub-01_dwi.bval'
else:
bvec_file = layout.get_bvec(dwi_file)
bval_file = layout.get_bval(dwi_file)
inputnode = pe.Node(niu.IdentityInterface(fields=[
'dwi_file', 'bvec_file', 'bval_file', 'subjects_dir', 'subject_id',
't1w_preproc', 't1w_brain', 't1w_mask', 't1w_dseg', 't1w_tpms',
't1w_aseg', 't1w_aparc', 'anat2std_xfm', 'std2anat_xfm', 'template',
'joint_anat2std_xfm', 'joint_std2anat_xfm', 'joint_template',
't1w2fsnative_xfm', 'fsnative2t1w_xfm'
]),
name='inputnode')
inputnode.inputs.dwi_file = dwi_file
inputnode.inputs.bvec_file = bvec_file
inputnode.inputs.bval_file = bval_file
outputnode = pe.Node(niu.IdentityInterface(
fields=['out_dwi', 'out_bvec', 'out_bval', 'out_rasb', 'out_dwi_mask'
]),
name='outputnode')
gradient_table = pe.Node(CheckGradientTable(), name='gradient_table')
dwi_reference_wf = init_dwi_reference_wf(omp_nthreads=1)
# MAIN WORKFLOW STRUCTURE
workflow.connect([
(inputnode, gradient_table, [('dwi_file', 'dwi_file'),
('bvec_file', 'in_bvec'),
('bval_file', 'in_bval')]),
(inputnode, dwi_reference_wf, [('dwi_file', 'inputnode.dwi_file')]),
(gradient_table, dwi_reference_wf, [('b0_ixs', 'inputnode.b0_ixs')]),
(dwi_reference_wf, outputnode, [('outputnode.ref_image', 'out_dwi'),
('outputnode.dwi_mask', 'out_dwi_mask')
]),
(gradient_table, outputnode, [('out_bvec', 'out_bvec'),
('out_bval', 'out_bval'),
('out_rasb', 'out_rasb')])
])
# REPORTING
reportlets_wf = init_reportlets_wf(reportlets_dir)
workflow.connect([
(inputnode, reportlets_wf, [('dwi_file', 'inputnode.source_file')]),
(dwi_reference_wf, reportlets_wf,
[('outputnode.ref_image', 'inputnode.dwi_ref'),
('outputnode.dwi_mask', 'inputnode.dwi_mask'),
('outputnode.validation_report', 'inputnode.validation_report')]),
])
return workflow
def init_func_preproc_wf(bold_file, ignore, freesurfer,
use_bbr, t2s_coreg, bold2t1w_dof, reportlets_dir,
output_spaces, template, output_dir, omp_nthreads,
fmap_bspline, fmap_demean, use_syn, force_syn,
use_aroma, ignore_aroma_err, aroma_melodic_dim,
medial_surface_nan, cifti_output,
debug, low_mem, template_out_grid,
layout=None, num_bold=1):
"""
This workflow controls the functional preprocessing stages of FMRIPREP.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.bold import init_func_preproc_wf
wf = init_func_preproc_wf('/completely/made/up/path/sub-01_task-nback_bold.nii.gz',
omp_nthreads=1,
ignore=[],
freesurfer=True,
reportlets_dir='.',
output_dir='.',
template='MNI152NLin2009cAsym',
output_spaces=['T1w', 'fsnative',
'template', 'fsaverage5'],
debug=False,
use_bbr=True,
t2s_coreg=False,
bold2t1w_dof=9,
fmap_bspline=True,
fmap_demean=True,
use_syn=True,
force_syn=True,
low_mem=False,
template_out_grid='native',
medial_surface_nan=False,
cifti_output=False,
use_aroma=False,
ignore_aroma_err=False,
aroma_melodic_dim=-200,
num_bold=1)
**Parameters**
bold_file : str
BOLD series NIfTI file
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
freesurfer : bool
Enable FreeSurfer functional registration (bbregister) and resampling
BOLD series to FreeSurfer surface meshes.
use_bbr : bool or None
Enable/disable boundary-based registration refinement.
If ``None``, test BBR result for distortion before accepting.
When using ``t2s_coreg``, BBR will be enabled by default unless
explicitly specified otherwise.
t2s_coreg : bool
For multiecho EPI, use the calculated T2*-map for T2*-driven coregistration
bold2t1w_dof : 6, 9 or 12
Degrees-of-freedom for BOLD-T1w registration
reportlets_dir : str
Directory in which to save reportlets
output_spaces : list
List of output spaces functional images are to be resampled to.
Some parts of pipeline will only be instantiated for some output spaces.
Valid spaces:
- T1w
- template
- fsnative
- fsaverage (or other pre-existing FreeSurfer templates)
template : str
Name of template targeted by ``template`` output space
output_dir : str
Directory in which to save derivatives
omp_nthreads : int
Maximum number of threads an individual process may use
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
force_syn : bool
**Temporary**: Always run SyN-based SDC
use_aroma : bool
Perform ICA-AROMA on MNI-resampled functional series
ignore_aroma_err : bool
Do not fail on ICA-AROMA errors
medial_surface_nan : bool
Replace medial wall values with NaNs on functional GIFTI files
cifti_output : bool
Generate bold CIFTI file in output spaces
debug : bool
Enable debugging outputs
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
template_out_grid : str
Keyword ('native', '1mm' or '2mm') or path of custom reference
image for normalization
layout : BIDSLayout
BIDSLayout structure to enable metadata retrieval
num_bold : int
Total number of BOLD files that have been set for preprocessing
(default is 1)
**Inputs**
bold_file
BOLD series NIfTI file
t1_preproc
Bias-corrected structural template image
t1_brain
Skull-stripped ``t1_preproc``
t1_mask
Mask of the skull-stripped template image
t1_seg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1_tpms
List of tissue probability maps in T1w space
t1_2_mni_forward_transform
ANTs-compatible affine-and-warp transform file
t1_2_mni_reverse_transform
ANTs-compatible affine-and-warp transform file (inverse)
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1_2_fsnative_forward_transform
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
t1_2_fsnative_reverse_transform
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
**Outputs**
bold_t1
BOLD series, resampled to T1w space
bold_mask_t1
BOLD series mask in T1w space
bold_mni
BOLD series, resampled to template space
bold_mask_mni
BOLD series mask in template space
confounds
TSV of confounds
surfaces
BOLD series, resampled to FreeSurfer surfaces
aroma_noise_ics
Noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
bold_cifti
BOLD CIFTI image
cifti_variant
combination of target spaces for `bold_cifti`
**Subworkflows**
* :py:func:`~fmriprep.workflows.bold.util.init_bold_reference_wf`
* :py:func:`~fmriprep.workflows.bold.stc.init_bold_stc_wf`
* :py:func:`~fmriprep.workflows.bold.hmc.init_bold_hmc_wf`
* :py:func:`~fmriprep.workflows.bold.t2s.init_bold_t2s_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_t1_trans_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_bold_confounds_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_ica_aroma_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_mni_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_preproc_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_surf_wf`
* :py:func:`~fmriprep.workflows.fieldmap.pepolar.init_pepolar_unwarp_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_fmap_estimator_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_sdc_unwarp_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_nonlinear_sdc_wf`
"""
from ..fieldmap.base import init_sdc_wf # Avoid circular dependency (#1066)
ref_file = bold_file
mem_gb = {'filesize': 1, 'resampled': 1, 'largemem': 1}
bold_tlen = 10
multiecho = isinstance(bold_file, list)
if multiecho:
tes = [layout.get_metadata(echo)['EchoTime'] for echo in bold_file]
ref_file = dict(zip(tes, bold_file))[min(tes)]
if os.path.isfile(ref_file):
bold_tlen, mem_gb = _create_mem_gb(ref_file)
wf_name = _get_wf_name(ref_file)
LOGGER.log(25, ('Creating bold processing workflow for "%s" (%.2f GB / %d TRs). '
'Memory resampled/largemem=%.2f/%.2f GB.'),
ref_file, mem_gb['filesize'], bold_tlen, mem_gb['resampled'], mem_gb['largemem'])
sbref_file = None
# For doc building purposes
if layout is None or bold_file == 'bold_preprocesing':
LOGGER.log(25, 'No valid layout: building empty workflow.')
metadata = {
'RepetitionTime': 2.0,
'SliceTiming': [0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9],
'PhaseEncodingDirection': 'j',
}
fmaps = [{
'type': 'phasediff',
'phasediff': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_phasediff.nii.gz',
'magnitude1': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude1.nii.gz',
'magnitude2': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude2.nii.gz',
}]
run_stc = True
multiecho = False
else:
# Find associated sbref, if possible
entities = layout.parse_file_entities(ref_file)
entities['type'] = 'sbref'
files = layout.get(**entities, extensions=['nii', 'nii.gz'])
refbase = os.path.basename(ref_file)
if 'sbref' in ignore:
LOGGER.info("Single-band reference files ignored.")
elif files and multiecho:
LOGGER.warning("Single-band reference found, but not supported in "
"multi-echo workflows at this time. Ignoring.")
elif files:
sbref_file = files[0].filename
sbbase = os.path.basename(sbref_file)
if len(files) > 1:
LOGGER.warning(
"Multiple single-band reference files found for {}; using "
"{}".format(refbase, sbbase))
else:
LOGGER.log(25, "Using single-band reference file {}".format(sbbase))
else:
LOGGER.log(25, "No single-band-reference found for {}".format(refbase))
metadata = layout.get_metadata(ref_file)
# Find fieldmaps. Options: (phase1|phase2|phasediff|epi|fieldmap|syn)
fmaps = []
if 'fieldmaps' not in ignore:
fmaps = layout.get_fieldmap(ref_file, return_list=True)
for fmap in fmaps:
fmap['metadata'] = layout.get_metadata(fmap[fmap['type']])
# Run SyN if forced or in the absence of fieldmap correction
if force_syn or (use_syn and not fmaps):
fmaps.append({'type': 'syn'})
# Short circuits: (True and True and (False or 'TooShort')) == 'TooShort'
run_stc = ("SliceTiming" in metadata and
'slicetiming' not in ignore and
(_get_series_len(ref_file) > 4 or "TooShort"))
# Check if MEEPI for T2* coregistration target
if t2s_coreg and not multiecho:
LOGGER.warning("No multiecho BOLD images found for T2* coregistration. "
"Using standard EPI-T1 coregistration.")
t2s_coreg = False
# By default, force-bbr for t2s_coreg unless user specifies otherwise
if t2s_coreg and use_bbr is None:
use_bbr = True
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__desc__ = """
Functional data preprocessing
: For each of the {num_bold} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""".format(num_bold=num_bold)
workflow.__postdesc__ = """\
All resamplings can be performed with *a single interpolation
step* by composing all the pertinent transformations (i.e. head-motion
transform matrices, susceptibility distortion correction when available,
and co-registrations to anatomical and template spaces).
Gridded (volumetric) resamplings were performed using `antsApplyTransforms` (ANTs),
configured with Lanczos interpolation to minimize the smoothing
effects of other kernels [@lanczos].
Non-gridded (surface) resamplings were performed using `mri_vol2surf`
(FreeSurfer).
"""
inputnode = pe.Node(niu.IdentityInterface(
fields=['bold_file', 'sbref_file', 'subjects_dir', 'subject_id',
't1_preproc', 't1_brain', 't1_mask', 't1_seg', 't1_tpms',
't1_aseg', 't1_aparc',
't1_2_mni_forward_transform', 't1_2_mni_reverse_transform',
't1_2_fsnative_forward_transform', 't1_2_fsnative_reverse_transform']),
name='inputnode')
inputnode.inputs.bold_file = bold_file
if sbref_file is not None:
inputnode.inputs.sbref_file = sbref_file
outputnode = pe.Node(niu.IdentityInterface(
fields=['bold_t1', 'bold_t1_ref', 'bold_mask_t1', 'bold_aseg_t1', 'bold_aparc_t1',
'bold_mni', 'bold_mni_ref' 'bold_mask_mni', 'bold_aseg_mni', 'bold_aparc_mni',
'bold_cifti', 'cifti_variant', 'cifti_variant_key', 'confounds', 'surfaces',
'aroma_noise_ics', 'melodic_mix', 'nonaggr_denoised_file']),
name='outputnode')
# BOLD buffer: an identity used as a pointer to either the original BOLD
# or the STC'ed one for further use.
boldbuffer = pe.Node(niu.IdentityInterface(fields=['bold_file']), name='boldbuffer')
summary = pe.Node(
FunctionalSummary(output_spaces=output_spaces,
slice_timing=run_stc,
registration='FreeSurfer' if freesurfer else 'FSL',
registration_dof=bold2t1w_dof,
pe_direction=metadata.get("PhaseEncodingDirection")),
name='summary', mem_gb=DEFAULT_MEMORY_MIN_GB, run_without_submitting=True)
func_derivatives_wf = init_func_derivatives_wf(output_dir=output_dir,
output_spaces=output_spaces,
template=template,
freesurfer=freesurfer,
use_aroma=use_aroma,
cifti_output=cifti_output)
workflow.connect([
(outputnode, func_derivatives_wf, [
('bold_t1', 'inputnode.bold_t1'),
('bold_t1_ref', 'inputnode.bold_t1_ref'),
('bold_aseg_t1', 'inputnode.bold_aseg_t1'),
('bold_aparc_t1', 'inputnode.bold_aparc_t1'),
('bold_mask_t1', 'inputnode.bold_mask_t1'),
('bold_mni', 'inputnode.bold_mni'),
('bold_mni_ref', 'inputnode.bold_mni_ref'),
('bold_aseg_mni', 'inputnode.bold_aseg_mni'),
('bold_aparc_mni', 'inputnode.bold_aparc_mni'),
('bold_mask_mni', 'inputnode.bold_mask_mni'),
('confounds', 'inputnode.confounds'),
('surfaces', 'inputnode.surfaces'),
('aroma_noise_ics', 'inputnode.aroma_noise_ics'),
('melodic_mix', 'inputnode.melodic_mix'),
('nonaggr_denoised_file', 'inputnode.nonaggr_denoised_file'),
('bold_cifti', 'inputnode.bold_cifti'),
('cifti_variant', 'inputnode.cifti_variant'),
('cifti_variant_key', 'inputnode.cifti_variant_key')
]),
])
# Generate a tentative boldref
bold_reference_wf = init_bold_reference_wf(omp_nthreads=omp_nthreads)
# Top-level BOLD splitter
bold_split = pe.Node(FSLSplit(dimension='t'), name='bold_split',
mem_gb=mem_gb['filesize'] * 3)
# HMC on the BOLD
bold_hmc_wf = init_bold_hmc_wf(name='bold_hmc_wf',
mem_gb=mem_gb['filesize'],
omp_nthreads=omp_nthreads)
# calculate BOLD registration to T1w
bold_reg_wf = init_bold_reg_wf(name='bold_reg_wf',
freesurfer=freesurfer,
use_bbr=use_bbr,
bold2t1w_dof=bold2t1w_dof,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# apply BOLD registration to T1w
bold_t1_trans_wf = init_bold_t1_trans_wf(name='bold_t1_trans_wf',
freesurfer=freesurfer,
use_fieldwarp=(fmaps is not None or use_syn),
multiecho=multiecho,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# get confounds
bold_confounds_wf = init_bold_confs_wf(
mem_gb=mem_gb['largemem'],
metadata=metadata,
name='bold_confounds_wf')
bold_confounds_wf.get_node('inputnode').inputs.t1_transform_flags = [False]
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_bold_trans_wf = init_bold_preproc_trans_wf(
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=not low_mem,
use_fieldwarp=(fmaps is not None or use_syn),
name='bold_bold_trans_wf'
)
bold_bold_trans_wf.inputs.inputnode.name_source = ref_file
# SLICE-TIME CORRECTION (or bypass) #############################################
if run_stc is True: # bool('TooShort') == True, so check True explicitly
bold_stc_wf = init_bold_stc_wf(name='bold_stc_wf', metadata=metadata)
workflow.connect([
(bold_reference_wf, bold_stc_wf, [
('outputnode.skip_vols', 'inputnode.skip_vols')]),
(bold_stc_wf, boldbuffer, [('outputnode.stc_file', 'bold_file')]),
])
if not multiecho:
workflow.connect([
(bold_reference_wf, bold_stc_wf, [
('outputnode.bold_file', 'inputnode.bold_file')])])
else: # for meepi, iterate through stc_wf for all workflows
meepi_echos = boldbuffer.clone(name='meepi_echos')
meepi_echos.iterables = ('bold_file', bold_file)
workflow.connect([
(meepi_echos, bold_stc_wf, [('bold_file', 'inputnode.bold_file')])])
elif not multiecho: # STC is too short or False
# bypass STC from original BOLD to the splitter through boldbuffer
workflow.connect([
(bold_reference_wf, boldbuffer, [('outputnode.bold_file', 'bold_file')])])
else:
# for meepi, iterate over all meepi echos to boldbuffer
boldbuffer.iterables = ('bold_file', bold_file)
# SDC (SUSCEPTIBILITY DISTORTION CORRECTION) or bypass ##########################
bold_sdc_wf = init_sdc_wf(
fmaps, metadata, omp_nthreads=omp_nthreads,
debug=debug, fmap_demean=fmap_demean, fmap_bspline=fmap_bspline)
bold_sdc_wf.inputs.inputnode.template = template
if not fmaps:
LOGGER.warning('SDC: no fieldmaps found or they were ignored (%s).',
ref_file)
elif fmaps[0]['type'] == 'syn':
LOGGER.warning(
'SDC: no fieldmaps found or they were ignored. '
'Using EXPERIMENTAL "fieldmap-less SyN" correction '
'for dataset %s.', ref_file)
else:
LOGGER.log(25, 'SDC: fieldmap estimation of type "%s" intended for %s found.',
fmaps[0]['type'], ref_file)
# MULTI-ECHO EPI DATA #############################################
if multiecho:
from .util import init_skullstrip_bold_wf
skullstrip_bold_wf = init_skullstrip_bold_wf(name='skullstrip_bold_wf')
inputnode.inputs.bold_file = ref_file # Replace reference w first echo
join_echos = pe.JoinNode(niu.IdentityInterface(fields=['bold_files']),
joinsource=('meepi_echos' if run_stc is True else 'boldbuffer'),
joinfield=['bold_files'],
name='join_echos')
# create optimal combination, adaptive T2* map
bold_t2s_wf = init_bold_t2s_wf(echo_times=tes,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
t2s_coreg=t2s_coreg,
name='bold_t2smap_wf')
workflow.connect([
(skullstrip_bold_wf, join_echos, [
('outputnode.skull_stripped_file', 'bold_files')]),
(join_echos, bold_t2s_wf, [
('bold_files', 'inputnode.bold_file')]),
])
# MAIN WORKFLOW STRUCTURE #######################################################
workflow.connect([
# Generate early reference
(inputnode, bold_reference_wf, [('bold_file', 'inputnode.bold_file'),
('sbref_file', 'inputnode.sbref_file')]),
# BOLD buffer has slice-time corrected if it was run, original otherwise
(boldbuffer, bold_split, [('bold_file', 'in_file')]),
# HMC
(bold_reference_wf, bold_hmc_wf, [
('outputnode.raw_ref_image', 'inputnode.raw_ref_image'),
('outputnode.bold_file', 'inputnode.bold_file')]),
# EPI-T1 registration workflow
(inputnode, bold_reg_wf, [
('t1_brain', 'inputnode.t1_brain'),
('t1_seg', 'inputnode.t1_seg'),
# Undefined if --no-freesurfer, but this is safe
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1_2_fsnative_reverse_transform', 'inputnode.t1_2_fsnative_reverse_transform')]),
(inputnode, bold_t1_trans_wf, [
('bold_file', 'inputnode.name_source'),
('t1_brain', 'inputnode.t1_brain'),
('t1_mask', 'inputnode.t1_mask'),
('t1_aseg', 'inputnode.t1_aseg'),
('t1_aparc', 'inputnode.t1_aparc')]),
# unused if multiecho, but this is safe
(bold_hmc_wf, bold_t1_trans_wf, [('outputnode.xforms', 'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_t1_trans_wf, [
('outputnode.itk_bold_to_t1', 'inputnode.itk_bold_to_t1')]),
(bold_t1_trans_wf, outputnode, [('outputnode.bold_t1', 'bold_t1'),
('outputnode.bold_t1_ref', 'bold_t1_ref'),
('outputnode.bold_aseg_t1', 'bold_aseg_t1'),
('outputnode.bold_aparc_t1', 'bold_aparc_t1')]),
(bold_reg_wf, summary, [('outputnode.fallback', 'fallback')]),
# SDC (or pass-through workflow)
(inputnode, bold_sdc_wf, [
('t1_brain', 'inputnode.t1_brain'),
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform')]),
(bold_reference_wf, bold_sdc_wf, [
('outputnode.ref_image', 'inputnode.bold_ref'),
('outputnode.ref_image_brain', 'inputnode.bold_ref_brain'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
# For t2s_coreg, replace EPI-to-T1w registration inputs
(bold_sdc_wf if not t2s_coreg else bold_t2s_wf, bold_reg_wf, [
('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain')]),
(bold_sdc_wf if not t2s_coreg else bold_t2s_wf, bold_t1_trans_wf, [
('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain'),
('outputnode.bold_mask', 'inputnode.ref_bold_mask')]),
(bold_sdc_wf, bold_t1_trans_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_sdc_wf, bold_bold_trans_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, summary, [('outputnode.method', 'distortion_correction')]),
# Connect bold_confounds_wf
(inputnode, bold_confounds_wf, [('t1_tpms', 'inputnode.t1_tpms'),
('t1_mask', 'inputnode.t1_mask')]),
(bold_hmc_wf, bold_confounds_wf, [
('outputnode.movpar_file', 'inputnode.movpar_file')]),
(bold_reg_wf, bold_confounds_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.t1_bold_xform')]),
(bold_reference_wf, bold_confounds_wf, [
('outputnode.skip_vols', 'inputnode.skip_vols')]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
# Connect bold_bold_trans_wf
(bold_split, bold_bold_trans_wf, [
('out_files', 'inputnode.bold_file')]),
(bold_hmc_wf, bold_bold_trans_wf, [
('outputnode.xforms', 'inputnode.hmc_xforms')]),
# Summary
(outputnode, summary, [('confounds', 'confounds_file')]),
])
# for standard EPI data, pass along correct file
if not multiecho:
workflow.connect([
(inputnode, func_derivatives_wf, [
('bold_file', 'inputnode.source_file')]),
(bold_bold_trans_wf, bold_confounds_wf, [
('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_split, bold_t1_trans_wf, [
('out_files', 'inputnode.bold_split')]),
])
else: # for meepi, create and use optimal combination
workflow.connect([
# update name source for optimal combination
(inputnode, func_derivatives_wf, [
(('bold_file', combine_meepi_source), 'inputnode.source_file')]),
(bold_bold_trans_wf, skullstrip_bold_wf, [
('outputnode.bold', 'inputnode.in_file')]),
(bold_t2s_wf, bold_confounds_wf, [
('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_t2s_wf, bold_t1_trans_wf, [
('outputnode.bold', 'inputnode.bold_split')]),
])
if fmaps:
from ..fieldmap.unwarp import init_fmap_unwarp_report_wf
sdc_type = fmaps[0]['type']
# Report on BOLD correction
fmap_unwarp_report_wf = init_fmap_unwarp_report_wf(
suffix='sdc_%s' % sdc_type)
workflow.connect([
(inputnode, fmap_unwarp_report_wf, [
('t1_seg', 'inputnode.in_seg')]),
(bold_reference_wf, fmap_unwarp_report_wf, [
('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, fmap_unwarp_report_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.in_xfm')]),
(bold_sdc_wf, fmap_unwarp_report_wf, [
('outputnode.bold_ref', 'inputnode.in_post')]),
])
if force_syn and sdc_type != 'syn':
syn_unwarp_report_wf = init_fmap_unwarp_report_wf(
suffix='forcedsyn', name='syn_unwarp_report_wf')
workflow.connect([
(inputnode, syn_unwarp_report_wf, [
('t1_seg', 'inputnode.in_seg')]),
(bold_reference_wf, syn_unwarp_report_wf, [
('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, syn_unwarp_report_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.in_xfm')]),
(bold_sdc_wf, syn_unwarp_report_wf, [
('outputnode.syn_bold_ref', 'inputnode.in_post')]),
])
# Map final BOLD mask into T1w space (if required)
if 'T1w' in output_spaces:
from niworkflows.interfaces.fixes import (
FixHeaderApplyTransforms as ApplyTransforms
)
boldmask_to_t1w = pe.Node(
ApplyTransforms(interpolation='MultiLabel', float=True),
name='boldmask_to_t1w', mem_gb=0.1
)
workflow.connect([
(bold_reg_wf, boldmask_to_t1w, [
('outputnode.itk_bold_to_t1', 'transforms')]),
(bold_t1_trans_wf, boldmask_to_t1w, [
('outputnode.bold_mask_t1', 'reference_image')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, boldmask_to_t1w, [
('outputnode.bold_mask', 'input_image')]),
(boldmask_to_t1w, outputnode, [
('output_image', 'bold_mask_t1')]),
])
if 'template' in output_spaces:
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_mni_trans_wf = init_bold_mni_trans_wf(
template=template,
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
template_out_grid=template_out_grid,
use_compression=not low_mem,
use_fieldwarp=fmaps is not None,
name='bold_mni_trans_wf'
)
carpetplot_wf = init_carpetplot_wf(
mem_gb=mem_gb['resampled'],
metadata=metadata,
name='carpetplot_wf')
workflow.connect([
(inputnode, bold_mni_trans_wf, [
('bold_file', 'inputnode.name_source'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform'),
('t1_aseg', 'inputnode.bold_aseg'),
('t1_aparc', 'inputnode.bold_aparc')]),
(bold_hmc_wf, bold_mni_trans_wf, [
('outputnode.xforms', 'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_mni_trans_wf, [
('outputnode.itk_bold_to_t1', 'inputnode.itk_bold_to_t1')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, bold_mni_trans_wf, [
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, bold_mni_trans_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_mni_trans_wf, outputnode, [('outputnode.bold_mni', 'bold_mni'),
('outputnode.bold_mni_ref', 'bold_mni_ref'),
('outputnode.bold_mask_mni', 'bold_mask_mni'),
('outputnode.bold_aseg_mni', 'bold_aseg_mni'),
('outputnode.bold_aparc_mni', 'bold_aparc_mni')]),
(inputnode, carpetplot_wf, [
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, carpetplot_wf, [
('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_reg_wf, carpetplot_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.t1_bold_xform')]),
(bold_confounds_wf, carpetplot_wf, [
('outputnode.confounds_file', 'inputnode.confounds_file')]),
])
if not multiecho:
workflow.connect([
(bold_split, bold_mni_trans_wf, [
('out_files', 'inputnode.bold_split')])
])
else:
split_opt_comb = bold_split.clone(name='split_opt_comb')
workflow.connect([
(bold_t2s_wf, split_opt_comb, [
('outputnode.bold', 'in_file')]),
(split_opt_comb, bold_mni_trans_wf, [
('out_files', 'inputnode.bold_split')
])
])
if use_aroma:
# ICA-AROMA workflow
# Internally resamples to MNI152 Linear (2006)
from .confounds import init_ica_aroma_wf
from niworkflows.interfaces.utils import JoinTSVColumns
ica_aroma_wf = init_ica_aroma_wf(
template=template,
metadata=metadata,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_fieldwarp=fmaps is not None,
ignore_aroma_err=ignore_aroma_err,
aroma_melodic_dim=aroma_melodic_dim,
name='ica_aroma_wf')
join = pe.Node(JoinTSVColumns(), name='aroma_confounds')
workflow.disconnect([
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
])
workflow.connect([
(inputnode, ica_aroma_wf, [
('bold_file', 'inputnode.name_source'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform')]),
(bold_split, ica_aroma_wf, [
('out_files', 'inputnode.bold_split')]),
(bold_hmc_wf, ica_aroma_wf, [
('outputnode.movpar_file', 'inputnode.movpar_file'),
('outputnode.xforms', 'inputnode.hmc_xforms')]),
(bold_reg_wf, ica_aroma_wf, [
('outputnode.itk_bold_to_t1', 'inputnode.itk_bold_to_t1')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, ica_aroma_wf, [
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, ica_aroma_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_reference_wf, ica_aroma_wf, [
('outputnode.skip_vols', 'inputnode.skip_vols')]),
(bold_confounds_wf, join, [
('outputnode.confounds_file', 'in_file')]),
(ica_aroma_wf, join,
[('outputnode.aroma_confounds', 'join_file')]),
(ica_aroma_wf, outputnode,
[('outputnode.aroma_noise_ics', 'aroma_noise_ics'),
('outputnode.melodic_mix', 'melodic_mix'),
('outputnode.nonaggr_denoised_file', 'nonaggr_denoised_file')]),
(join, outputnode, [('out_file', 'confounds')]),
])
# SURFACES ##################################################################################
surface_spaces = [space for space in output_spaces if space.startswith('fs')]
if freesurfer and surface_spaces:
LOGGER.log(25, 'Creating BOLD surface-sampling workflow.')
bold_surf_wf = init_bold_surf_wf(mem_gb=mem_gb['resampled'],
output_spaces=surface_spaces,
medial_surface_nan=medial_surface_nan,
name='bold_surf_wf')
workflow.connect([
(inputnode, bold_surf_wf, [
('t1_preproc', 'inputnode.t1_preproc'),
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1_2_fsnative_forward_transform', 'inputnode.t1_2_fsnative_forward_transform')]),
(bold_t1_trans_wf, bold_surf_wf, [('outputnode.bold_t1', 'inputnode.source_file')]),
(bold_surf_wf, outputnode, [('outputnode.surfaces', 'surfaces')]),
])
# CIFTI output
if cifti_output and surface_spaces:
bold_surf_wf.__desc__ += """\
*Grayordinates* files [@hcppipelines], which combine surface-sampled
data and volume-sampled data, were also generated.
"""
gen_cifti = pe.MapNode(GenerateCifti(), iterfield=["surface_target", "gifti_files"],
name="gen_cifti")
gen_cifti.inputs.TR = metadata.get("RepetitionTime")
gen_cifti.inputs.surface_target = [s for s in surface_spaces
if s.startswith('fsaverage')]
workflow.connect([
(bold_surf_wf, gen_cifti, [
('outputnode.surfaces', 'gifti_files')]),
(inputnode, gen_cifti, [('subjects_dir', 'subjects_dir')]),
(bold_mni_trans_wf, gen_cifti, [('outputnode.bold_mni', 'bold_file')]),
(gen_cifti, outputnode, [('out_file', 'bold_cifti'),
('variant', 'cifti_variant'),
('variant_key', 'cifti_variant_key')]),
])
# REPORTING ############################################################
ds_report_summary = pe.Node(
DerivativesDataSink(suffix='summary'),
name='ds_report_summary', run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
ds_report_validation = pe.Node(
DerivativesDataSink(base_directory=reportlets_dir,
suffix='validation'),
name='ds_report_validation', run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
workflow.connect([
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bold_reference_wf, ds_report_validation, [
('outputnode.validation_report', 'in_file')]),
])
# Fill-in datasinks of reportlets seen so far
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_report'):
workflow.get_node(node).inputs.base_directory = reportlets_dir
workflow.get_node(node).inputs.source_file = ref_file
return workflow
def init_topup_wf(omp_nthreads=1, debug=False, name="pepolar_estimate_wf"):
"""
Create the PEPOLAR field estimation workflow based on FSL's ``topup``.
Workflow Graph
.. workflow ::
:graph2use: orig
:simple_form: yes
from sdcflows.workflows.fit.pepolar import init_topup_wf
wf = init_topup_wf()
Parameters
----------
debug : :obj:`bool`
Whether a fast configuration of topup (less accurate) should be applied.
name : :obj:`str`
Name for this workflow
omp_nthreads : :obj:`int`
Parallelize internal tasks across the number of CPUs given by this option.
Inputs
------
in_data : :obj:`list` of :obj:`str`
A list of EPI files that will be fed into TOPUP.
metadata : :obj:`list` of :obj:`dict`
A list of dictionaries containing the metadata corresponding to each file
in ``in_data``.
Outputs
-------
fmap : :obj:`str`
The path of the estimated fieldmap.
fmap_ref : :obj:`str`
The path of an unwarped conversion of files in ``in_data``.
fmap_mask : :obj:`str`
The path of mask corresponding to the ``fmap_ref`` output.
fmap_coeff : :obj:`str` or :obj:`list` of :obj:`str`
The path(s) of the B-Spline coefficients supporting the fieldmap.
"""
from nipype.interfaces.fsl.epi import TOPUP
from niworkflows.interfaces.nibabel import MergeSeries
from niworkflows.interfaces.images import IntraModalMerge
from ...interfaces.epi import GetReadoutTime
from ...interfaces.utils import Flatten
from ...interfaces.bspline import TOPUPCoeffReorient
from ..ancillary import init_brainextraction_wf
workflow = Workflow(name=name)
workflow.__postdesc__ = f"""\
{_PEPOLAR_DESC} with `topup` (@topup; FSL {TOPUP().version}).
"""
inputnode = pe.Node(niu.IdentityInterface(fields=INPUT_FIELDS),
name="inputnode")
outputnode = pe.Node(
niu.IdentityInterface(fields=[
"fmap",
"fmap_ref",
"fmap_coeff",
"fmap_mask",
"jacobians",
"xfms",
"out_warps",
]),
name="outputnode",
)
flatten = pe.Node(Flatten(), name="flatten")
concat_blips = pe.Node(MergeSeries(), name="concat_blips")
readout_time = pe.MapNode(
GetReadoutTime(),
name="readout_time",
iterfield=["metadata", "in_file"],
run_without_submitting=True,
)
topup = pe.Node(
TOPUP(config=_pkg_fname("sdcflows",
f"data/flirtsch/b02b0{'_quick' * debug}.cnf")),
name="topup",
)
merge_corrected = pe.Node(IntraModalMerge(hmc=False, to_ras=False),
name="merge_corrected")
fix_coeff = pe.Node(TOPUPCoeffReorient(),
name="fix_coeff",
run_without_submitting=True)
brainextraction_wf = init_brainextraction_wf()
# fmt: off
workflow.connect([
(inputnode, flatten, [("in_data", "in_data"),
("metadata", "in_meta")]),
(flatten, readout_time, [("out_data", "in_file"),
("out_meta", "metadata")]),
(flatten, concat_blips, [("out_data", "in_files")]),
(flatten, topup, [(("out_meta", _pe2fsl), "encoding_direction")]),
(readout_time, topup, [("readout_time", "readout_times")]),
(concat_blips, topup, [("out_file", "in_file")]),
(topup, merge_corrected, [("out_corrected", "in_files")]),
(topup, fix_coeff, [("out_fieldcoef", "in_coeff"),
("out_corrected", "fmap_ref")]),
(topup, outputnode, [("out_field", "fmap"), ("out_jacs", "jacobians"),
("out_mats", "xfms"),
("out_warps", "out_warps")]),
(merge_corrected, brainextraction_wf, [("out_avg", "inputnode.in_file")
]),
(merge_corrected, outputnode, [("out_avg", "fmap_ref")]),
(brainextraction_wf, outputnode, [("outputnode.out_mask", "fmap_mask")
]),
(fix_coeff, outputnode, [("out_coeff", "fmap_coeff")]),
])
# fmt: on
return workflow
def init_sdc_wf(fmaps, bold_meta, omp_nthreads=1,
debug=False, fmap_bspline=False, fmap_demean=True):
"""
This workflow implements the heuristics to choose a
:abbr:`SDC (susceptibility distortion correction)` strategy.
When no field map information is present within the BIDS inputs,
the EXPERIMENTAL "fieldmap-less SyN" can be performed, using
the ``--use-syn`` argument. When ``--force-syn`` is specified,
then the "fieldmap-less SyN" is always executed and reported
despite of other fieldmaps available with higher priority.
In the latter case (some sort of fieldmap(s) is available and
``--force-syn`` is requested), then the :abbr:`SDC (susceptibility
distortion correction)` method applied is that with the
highest priority.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.fieldmap import init_sdc_wf
wf = init_sdc_wf(
fmaps=[{
'type': 'phasediff',
'phasediff': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_phasediff.nii.gz',
'magnitude1': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude1.nii.gz',
'magnitude2': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude2.nii.gz',
}],
bold_meta={
'RepetitionTime': 2.0,
'SliceTiming': [0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9],
'PhaseEncodingDirection': 'j',
},
)
**Parameters**
fmaps : list of pybids dicts
A list of dictionaries with the available fieldmaps
(and their metadata using the key ``'metadata'`` for the
case of *epi* fieldmaps)
bold_meta : dict
BIDS metadata dictionary corresponding to the BOLD run
omp_nthreads : int
Maximum number of threads an individual process may use
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
debug : bool
Enable debugging outputs
**Inputs**
bold_ref
A BOLD reference calculated at a previous stage
bold_ref_brain
Same as above, but brain-masked
bold_mask
Brain mask for the BOLD run
t1_brain
T1w image, brain-masked, for the fieldmap-less SyN method
t1_2_mni_reverse_transform
MNI-to-T1w transform to map prior knowledge to the T1w
fo the fieldmap-less SyN method
template : str
Name of template targeted by ``template`` output space
**Outputs**
bold_ref
An unwarped BOLD reference
bold_mask
The corresponding new mask after unwarping
bold_ref_brain
Brain-extracted, unwarped BOLD reference
out_warp
The deformation field to unwarp the susceptibility distortions
syn_bold_ref
If ``--force-syn``, an unwarped BOLD reference with this
method (for reporting purposes)
"""
# TODO: To be removed (filter out unsupported fieldmaps):
fmaps = [fmap for fmap in fmaps if fmap['type'] in FMAP_PRIORITY]
workflow = Workflow(name='sdc_wf' if fmaps else 'sdc_bypass_wf')
inputnode = pe.Node(niu.IdentityInterface(
fields=['bold_ref', 'bold_ref_brain', 'bold_mask',
't1_brain', 't1_2_mni_reverse_transform', 'template']),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(
fields=['bold_ref', 'bold_mask', 'bold_ref_brain',
'out_warp', 'syn_bold_ref', 'method']),
name='outputnode')
# No fieldmaps - forward inputs to outputs
if not fmaps:
outputnode.inputs.method = 'None'
workflow.connect([
(inputnode, outputnode, [('bold_ref', 'bold_ref'),
('bold_mask', 'bold_mask'),
('bold_ref_brain', 'bold_ref_brain')]),
])
return workflow
workflow.__postdesc__ = """\
Based on the estimated susceptibility distortion, an
unwarped BOLD reference was calculated for a more accurate
co-registration with the anatomical reference.
"""
# In case there are multiple fieldmaps prefer EPI
fmaps.sort(key=lambda fmap: FMAP_PRIORITY[fmap['type']])
fmap = fmaps[0]
# PEPOLAR path
if fmap['type'] == 'epi':
outputnode.inputs.method = 'PEB/PEPOLAR (phase-encoding based / PE-POLARity)'
# Get EPI polarities and their metadata
epi_fmaps = [(fmap_['epi'], fmap_['metadata']["PhaseEncodingDirection"])
for fmap_ in fmaps if fmap_['type'] == 'epi']
sdc_unwarp_wf = init_pepolar_unwarp_wf(
bold_meta=bold_meta,
epi_fmaps=epi_fmaps,
omp_nthreads=omp_nthreads,
name='pepolar_unwarp_wf')
workflow.connect([
(inputnode, sdc_unwarp_wf, [
('bold_ref', 'inputnode.in_reference'),
('bold_mask', 'inputnode.in_mask'),
('bold_ref_brain', 'inputnode.in_reference_brain')]),
])
# FIELDMAP path
if fmap['type'] in ['fieldmap', 'phasediff']:
outputnode.inputs.method = 'FMB (%s-based)' % fmap['type']
# Import specific workflows here, so we don't break everything with one
# unused workflow.
if fmap['type'] == 'fieldmap':
from .fmap import init_fmap_wf
fmap_estimator_wf = init_fmap_wf(
omp_nthreads=omp_nthreads,
fmap_bspline=fmap_bspline)
# set inputs
fmap_estimator_wf.inputs.inputnode.fieldmap = fmap['fieldmap']
fmap_estimator_wf.inputs.inputnode.magnitude = fmap['magnitude']
if fmap['type'] == 'phasediff':
from .phdiff import init_phdiff_wf
fmap_estimator_wf = init_phdiff_wf(omp_nthreads=omp_nthreads)
# set inputs
fmap_estimator_wf.inputs.inputnode.phasediff = fmap['phasediff']
fmap_estimator_wf.inputs.inputnode.magnitude = [
fmap_ for key, fmap_ in sorted(fmap.items())
if key.startswith("magnitude")
]
sdc_unwarp_wf = init_sdc_unwarp_wf(
omp_nthreads=omp_nthreads,
fmap_demean=fmap_demean,
debug=debug,
name='sdc_unwarp_wf')
sdc_unwarp_wf.inputs.inputnode.metadata = bold_meta
workflow.connect([
(inputnode, sdc_unwarp_wf, [
('bold_ref', 'inputnode.in_reference'),
('bold_ref_brain', 'inputnode.in_reference_brain'),
('bold_mask', 'inputnode.in_mask')]),
(fmap_estimator_wf, sdc_unwarp_wf, [
('outputnode.fmap', 'inputnode.fmap'),
('outputnode.fmap_ref', 'inputnode.fmap_ref'),
('outputnode.fmap_mask', 'inputnode.fmap_mask')]),
])
# FIELDMAP-less path
if any(fm['type'] == 'syn' for fm in fmaps):
syn_sdc_wf = init_syn_sdc_wf(
bold_pe=bold_meta.get('PhaseEncodingDirection', None),
omp_nthreads=omp_nthreads)
workflow.connect([
(inputnode, syn_sdc_wf, [
('t1_brain', 'inputnode.t1_brain'),
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform'),
('bold_ref', 'inputnode.bold_ref'),
('bold_ref_brain', 'inputnode.bold_ref_brain'),
('template', 'inputnode.template')]),
])
# XXX Eliminate branch when forcing isn't an option
if fmap['type'] == 'syn': # No fieldmaps, but --use-syn
outputnode.inputs.method = 'FLB ("fieldmap-less", SyN-based)'
sdc_unwarp_wf = syn_sdc_wf
else: # --force-syn was called when other fieldmap was present
sdc_unwarp_wf.__desc__ = None
workflow.connect([
(syn_sdc_wf, outputnode, [
('outputnode.out_reference', 'syn_bold_ref')]),
])
workflow.connect([
(sdc_unwarp_wf, outputnode, [
('outputnode.out_warp', 'out_warp'),
('outputnode.out_reference', 'bold_ref'),
('outputnode.out_reference_brain', 'bold_ref_brain'),
('outputnode.out_mask', 'bold_mask')]),
])
return workflow
def init_single_subject_wf(subject_id):
"""
Organize the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from nibabies.workflows.tests import mock_config
from nibabies.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf('01')
Parameters
----------
subject_id : :obj:`str`
Subject label for this single-subject workflow.
Inputs
------
subjects_dir : :obj:`str`
FreeSurfer's ``$SUBJECTS_DIR``.
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.bids import BIDSInfo, BIDSDataGrabber
from niworkflows.interfaces.nilearn import NILEARN_VERSION
from niworkflows.utils.bids import collect_data
from niworkflows.utils.spaces import Reference
from .anatomical import init_infant_anat_wf
from ..utils.misc import fix_multi_source_name
name = "single_subject_%s_wf" % subject_id
subject_data = collect_data(
config.execution.layout,
subject_id,
config.execution.task_id,
config.execution.echo_idx,
bids_filters=config.execution.bids_filters,
)[0]
if "flair" in config.workflow.ignore:
subject_data["flair"] = []
if "t2w" in config.workflow.ignore:
subject_data["t2w"] = []
anat_only = config.workflow.anat_only
anat_derivatives = config.execution.anat_derivatives
anat_modality = config.workflow.anat_modality
spaces = config.workflow.spaces
# Make sure we always go through these two checks
if not anat_only and not subject_data["bold"]:
task_id = config.execution.task_id
raise RuntimeError(
"No BOLD images found for participant {} and task {}. "
"All workflows require BOLD images.".format(
subject_id, task_id if task_id else "<all>"))
if anat_derivatives:
from smriprep.utils.bids import collect_derivatives
std_spaces = spaces.get_spaces(nonstandard=False, dim=(3, ))
anat_derivatives = collect_derivatives(
anat_derivatives.absolute(),
subject_id,
std_spaces,
config.workflow.run_reconall,
)
if anat_derivatives is None:
config.loggers.workflow.warning(f"""\
Attempted to access pre-existing anatomical derivatives at \
<{config.execution.anat_derivatives}>, however not all expectations of fMRIPrep \
were met (for participant <{subject_id}>, spaces <{', '.join(std_spaces)}>, \
reconall <{config.workflow.run_reconall}>).""")
if not anat_derivatives and not subject_data[anat_modality]:
raise Exception(
f"No {anat_modality} images found for participant {subject_id}. "
"All workflows require T1w images.")
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPrep* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(
fmriprep_ver=config.environment.version,
nipype_ver=config.environment.nipype_version,
)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://nibabies.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by fMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(nilearn_ver=NILEARN_VERSION)
nibabies_dir = str(config.execution.nibabies_dir)
inputnode = pe.Node(niu.IdentityInterface(fields=["subjects_dir"]),
name="inputnode")
bidssrc = pe.Node(
BIDSDataGrabber(
subject_data=subject_data,
anat_only=anat_only,
anat_derivatives=anat_derivatives,
subject_id=subject_id,
),
name="bidssrc",
)
bids_info = pe.Node(
BIDSInfo(bids_dir=config.execution.bids_dir, bids_validate=False),
name="bids_info",
)
summary = pe.Node(
SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False),
),
name="summary",
run_without_submitting=True,
)
about = pe.Node(
AboutSummary(version=config.environment.version,
command=" ".join(sys.argv)),
name="about",
run_without_submitting=True,
)
ds_report_summary = pe.Node(
DerivativesDataSink(
base_directory=nibabies_dir,
desc="summary",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_summary",
run_without_submitting=True,
)
ds_report_about = pe.Node(
DerivativesDataSink(
base_directory=nibabies_dir,
desc="about",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_about",
run_without_submitting=True,
)
# Preprocessing of anatomical (includes registration to UNCInfant)
anat_preproc_wf = init_infant_anat_wf(
ants_affine_init=config.workflow.ants_affine_init or True,
age_months=config.workflow.age_months,
anat_modality=anat_modality,
t1w=subject_data["t1w"],
t2w=subject_data["t2w"],
bids_root=config.execution.bids_dir,
existing_derivatives=anat_derivatives,
freesurfer=config.workflow.run_reconall,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=nibabies_dir,
segmentation_atlases=config.execution.segmentation_atlases_dir,
skull_strip_mode=config.workflow.skull_strip_t1w,
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
sloppy=config.execution.sloppy,
spaces=spaces,
)
# fmt: off
workflow.connect([
(inputnode, anat_preproc_wf, [
('subjects_dir', 'inputnode.subjects_dir'),
]),
(inputnode, summary, [
('subjects_dir', 'subjects_dir'),
]),
(bidssrc, summary, [
('bold', 'bold'),
]),
(bids_info, summary, [
('subject', 'subject_id'),
]),
(bids_info, anat_preproc_wf, [
(('subject', _prefix), 'inputnode.subject_id'),
]),
(
bidssrc,
anat_preproc_wf,
[
('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
# ('roi', 'inputnode.roi'),
# ('flair', 'inputnode.flair'),
]),
(summary, ds_report_summary, [
('out_report', 'in_file'),
]),
(about, ds_report_about, [
('out_report', 'in_file'),
]),
])
if not anat_derivatives:
workflow.connect([
(bidssrc, bids_info, [
(('t1w', fix_multi_source_name), 'in_file'),
]),
(bidssrc, summary, [
('t1w', 't1w'),
('t2w', 't2w'),
]),
(bidssrc, ds_report_summary, [
(('t1w', fix_multi_source_name), 'source_file'),
]),
(bidssrc, ds_report_about, [
(('t1w', fix_multi_source_name), 'source_file'),
]),
])
else:
workflow.connect([
(bidssrc, bids_info, [
(('bold', fix_multi_source_name), 'in_file'),
]),
(anat_preproc_wf, summary, [
('outputnode.t1w_preproc', 't1w'),
]),
(anat_preproc_wf, ds_report_summary, [
('outputnode.t1w_preproc', 'source_file'),
]),
(anat_preproc_wf, ds_report_about, [
('outputnode.t1w_preproc', 'source_file'),
]),
])
# fmt: on
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split(".")[-1].startswith("ds_"):
workflow.get_node(node).interface.out_path_base = ""
if anat_only:
return workflow
# Susceptibility distortion correction
fmap_estimators = None
if "fieldmap" not in config.workflow.ignore:
from sdcflows.utils.wrangler import find_estimators
from sdcflows.workflows.base import init_fmap_preproc_wf
# SDC Step 1: Run basic heuristics to identify available data for fieldmap estimation
# For now, no fmapless
fmap_estimators = find_estimators(
layout=config.execution.layout,
subject=subject_id,
fmapless=False, # config.workflow.use_syn,
force_fmapless=False, # config.workflow.force_syn,
)
# Append the functional section to the existing anatomical exerpt
# That way we do not need to stream down the number of bold datasets
anat_preproc_wf.__postdesc__ = ((anat_preproc_wf.__postdesc__ if hasattr(
anat_preproc_wf, '__postdesc__') else "") + f"""
Functional data preprocessing
: For each of the {len(subject_data['bold'])} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""")
# calculate reference image(s) for BOLD images
# group all BOLD files based on same:
# 1) session
# 2) PE direction
# 3) total readout time
from niworkflows.workflows.epi.refmap import init_epi_reference_wf
_, bold_groupings = group_bolds_ref(layout=config.execution.layout,
subject=subject_id)
if any(not x for x in bold_groupings):
print("No BOLD files found for one or more reference groupings")
return workflow
func_preproc_wfs = []
for idx, bold_files in enumerate(bold_groupings):
bold_ref_wf = init_epi_reference_wf(
auto_bold_nss=True,
name=f'bold_reference_wf{idx}',
omp_nthreads=config.nipype.omp_nthreads)
bold_ref_wf.inputs.inputnode.in_files = bold_files
for idx, bold_file in enumerate(bold_files):
func_preproc_wf = init_func_preproc_wf(
bold_file, has_fieldmap=bool(fmap_estimators))
# fmt: off
workflow.connect([
(bold_ref_wf, func_preproc_wf, [
('outputnode.epi_ref_file', 'inputnode.bold_ref'),
(('outputnode.xfm_files', _select_iter_idx, idx),
'inputnode.bold_ref_xfm'),
(('outputnode.n_dummy', _select_iter_idx, idx),
'inputnode.n_dummy_scans'),
]),
(
anat_preproc_wf,
func_preproc_wf,
[
('outputnode.anat_preproc', 'inputnode.anat_preproc'),
('outputnode.anat_mask', 'inputnode.anat_mask'),
('outputnode.anat_brain', 'inputnode.anat_brain'),
('outputnode.anat_dseg', 'inputnode.anat_dseg'),
('outputnode.anat_aseg', 'inputnode.anat_aseg'),
('outputnode.anat_aparc', 'inputnode.anat_aparc'),
('outputnode.anat_tpms', 'inputnode.anat_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm'),
# Undefined if --fs-no-reconall, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.anat2fsnative_xfm',
'inputnode.anat2fsnative_xfm'),
('outputnode.fsnative2anat_xfm',
'inputnode.fsnative2anat_xfm'),
]),
])
# fmt: on
func_preproc_wfs.append(func_preproc_wf)
if not fmap_estimators:
config.loggers.workflow.warning(
"Data for fieldmap estimation not present. Please note that these data "
"will not be corrected for susceptibility distortions.")
return workflow
config.loggers.workflow.info(
f"Fieldmap estimators found: {[e.method for e in fmap_estimators]}")
from sdcflows.workflows.base import init_fmap_preproc_wf
from sdcflows import fieldmaps as fm
fmap_wf = init_fmap_preproc_wf(
debug=bool(
config.execution.debug), # TODO: Add debug option for fieldmaps
estimators=fmap_estimators,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=nibabies_dir,
subject=subject_id,
)
fmap_wf.__desc__ = f"""
Fieldmap data preprocessing
: A total of {len(fmap_estimators)} fieldmaps were found available within the input
BIDS structure for this particular subject.
"""
for func_preproc_wf in func_preproc_wfs:
# fmt: off
workflow.connect([
(fmap_wf, func_preproc_wf, [
("outputnode.fmap", "inputnode.fmap"),
("outputnode.fmap_ref", "inputnode.fmap_ref"),
("outputnode.fmap_coeff", "inputnode.fmap_coeff"),
("outputnode.fmap_mask", "inputnode.fmap_mask"),
("outputnode.fmap_id", "inputnode.fmap_id"),
]),
])
# fmt: on
# Overwrite ``out_path_base`` of sdcflows's DataSinks
for node in fmap_wf.list_node_names():
if node.split(".")[-1].startswith("ds_"):
fmap_wf.get_node(node).interface.out_path_base = ""
# Step 3: Manually connect PEPOLAR
for estimator in fmap_estimators:
config.loggers.workflow.info(f"""\
Setting-up fieldmap "{estimator.bids_id}" ({estimator.method}) with \
<{', '.join(s.path.name for s in estimator.sources)}>""")
if estimator.method in (fm.EstimatorType.MAPPED,
fm.EstimatorType.PHASEDIFF):
continue
suffices = set(s.suffix for s in estimator.sources)
if estimator.method == fm.EstimatorType.PEPOLAR and sorted(
suffices) == ["epi"]:
getattr(fmap_wf.inputs, f"in_{estimator.bids_id}").in_data = [
str(s.path) for s in estimator.sources
]
getattr(fmap_wf.inputs, f"in_{estimator.bids_id}").metadata = [
s.metadata for s in estimator.sources
]
continue
if estimator.method == fm.EstimatorType.PEPOLAR:
raise NotImplementedError(
"Sophisticated PEPOLAR schemes (e.g., using DWI+EPI) are unsupported."
)
return workflow
def init_single_subject_wf(subject_id):
"""
Set-up the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and diffusion MRI preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Diffusion MRI preprocessing is performed using a separate workflow for
a full :abbr:`DWI (diffusion weighted imaging)` *entity*.
A DWI *entity* may comprehend one or several runs (for instance, two
opposed :abbr:`PE (phase-encoding)` directions.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from dmriprep.config.testing import mock_config
from dmriprep.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf("THP0005")
Parameters
----------
subject_id : str
List of subject labels
Inputs
------
subjects_dir : os.pathlike
FreeSurfer's ``$SUBJECTS_DIR``
"""
from ..utils.misc import sub_prefix as _prefix
name = f"single_subject_{subject_id}_wf"
subject_data = collect_data(config.execution.layout, subject_id)[0]
if "flair" in config.workflow.ignore:
subject_data["flair"] = []
if "t2w" in config.workflow.ignore:
subject_data["t2w"] = []
anat_only = config.workflow.anat_only
# Make sure we always go through these two checks
if not anat_only and not subject_data["dwi"]:
raise Exception(f"No DWI data found for participant {subject_id}. "
"All workflows require DWI images.")
if not subject_data["t1w"]:
raise Exception(f"No T1w images found for participant {subject_id}. "
"All workflows require T1w images.")
workflow = Workflow(name=name)
workflow.__desc__ = f"""
Results included in this manuscript come from preprocessing
performed using *dMRIPrep* {config.environment.version}
(@dmriprep; RRID:SCR_017412),
which is based on *Nipype* {config.environment.nipype_version}
(@nipype1; @nipype2; RRID:SCR_002502).
"""
workflow.__postdesc__ = """
For more details of the pipeline, see [the section corresponding
to workflows in *dMRIPrep*'s documentation]\
(https://nipreps.github.io/dmriprep/master/workflows.html \
"dMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by dMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
"""
spaces = config.workflow.spaces
output_dir = config.execution.output_dir
fsinputnode = pe.Node(niu.IdentityInterface(fields=["subjects_dir"]),
name="fsinputnode")
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only),
name="bidssrc")
bids_info = pe.Node(
BIDSInfo(bids_dir=config.execution.bids_dir, bids_validate=False),
name="bids_info",
)
summary = pe.Node(
SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False),
),
name="summary",
run_without_submitting=True,
)
about = pe.Node(
AboutSummary(version=config.environment.version,
command=" ".join(sys.argv)),
name="about",
run_without_submitting=True,
)
ds_report_summary = pe.Node(
DerivativesDataSink(base_directory=str(output_dir),
desc="summary",
datatype="figures"),
name="ds_report_summary",
run_without_submitting=True,
)
ds_report_about = pe.Node(
DerivativesDataSink(base_directory=str(output_dir),
desc="about",
datatype="figures"),
name="ds_report_about",
run_without_submitting=True,
)
anat_derivatives = config.execution.anat_derivatives
if anat_derivatives:
from smriprep.utils.bids import collect_derivatives
std_spaces = spaces.get_spaces(nonstandard=False, dim=(3, ))
anat_derivatives = collect_derivatives(
anat_derivatives.absolute(),
subject_id,
std_spaces,
config.workflow.run_reconall,
)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=str(config.execution.bids_dir),
debug=config.execution.debug is True,
existing_derivatives=anat_derivatives,
freesurfer=config.workflow.run_reconall,
hires=config.workflow.hires,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=str(output_dir),
skull_strip_fixed_seed=config.workflow.skull_strip_fixed_seed,
skull_strip_mode="force",
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
spaces=spaces,
t1w=subject_data["t1w"],
)
anat_preproc_wf.__desc__ = f"\n\n{anat_preproc_wf.__desc__}"
# fmt:off
workflow.connect([
(fsinputnode, anat_preproc_wf, [("subjects_dir",
"inputnode.subjects_dir")]),
(bidssrc, bids_info, [(("t1w", fix_multi_T1w_source_name), "in_file")
]),
(fsinputnode, summary, [("subjects_dir", "subjects_dir")]),
(bidssrc, summary, [("t1w", "t1w"), ("t2w", "t2w"), ("dwi", "dwi")]),
(bids_info, summary, [("subject", "subject_id")]),
(bids_info, anat_preproc_wf, [(("subject", _prefix),
"inputnode.subject_id")]),
(bidssrc, anat_preproc_wf, [
("t1w", "inputnode.t1w"),
("t2w", "inputnode.t2w"),
("roi", "inputnode.roi"),
("flair", "inputnode.flair"),
]),
(bidssrc, ds_report_summary, [
(("t1w", fix_multi_T1w_source_name), "source_file"),
]),
(summary, ds_report_summary, [("out_report", "in_file")]),
(bidssrc, ds_report_about, [(("t1w", fix_multi_T1w_source_name),
"source_file")]),
(about, ds_report_about, [("out_report", "in_file")]),
])
# fmt:off
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split(".")[-1].startswith("ds_"):
workflow.get_node(node).interface.out_path_base = "dmriprep"
if anat_only:
return workflow
from .dwi.base import init_dwi_preproc_wf
# Append the dMRI section to the existing anatomical excerpt
# That way we do not need to stream down the number of DWI datasets
anat_preproc_wf.__postdesc__ = f"""\
{anat_preproc_wf.__postdesc__ or ''}
Diffusion data preprocessing
: For each of the {len(subject_data["dwi"])} DWI scans found per subject
(across all sessions), the gradient table was vetted and converted into the *RASb*
format (i.e., given in RAS+ scanner coordinates, normalized b-vectors and scaled b-values),
and a *b=0* average for reference to the subsequent steps of preprocessing was calculated.
"""
# SDC Step 0: Determine whether fieldmaps can/should be estimated
fmap_estimators = None
if "fieldmap" not in config.workflow.ignore:
from sdcflows import fieldmaps as fm
from sdcflows.utils.wrangler import find_estimators
from sdcflows.workflows.base import init_fmap_preproc_wf
# SDC Step 1: Run basic heuristics to identify available data for fieldmap estimation
fmap_estimators = find_estimators(
layout=config.execution.layout,
subject=subject_id,
fmapless=False,
)
# Add fieldmap-less estimators
if not fmap_estimators and config.workflow.use_syn:
# estimators = [fm.FieldmapEstimation()]
raise NotImplementedError
# Nuts and bolts: initialize individual run's pipeline
dwi_preproc_list = []
for dwi_file in subject_data["dwi"]:
dwi_preproc_wf = init_dwi_preproc_wf(
dwi_file,
has_fieldmap=bool(fmap_estimators),
)
# fmt: off
workflow.connect([
(
anat_preproc_wf,
dwi_preproc_wf,
[
("outputnode.t1w_preproc", "inputnode.t1w_preproc"),
("outputnode.t1w_mask", "inputnode.t1w_mask"),
("outputnode.t1w_dseg", "inputnode.t1w_dseg"),
("outputnode.t1w_aseg", "inputnode.t1w_aseg"),
("outputnode.t1w_aparc", "inputnode.t1w_aparc"),
("outputnode.t1w_tpms", "inputnode.t1w_tpms"),
("outputnode.template", "inputnode.template"),
("outputnode.anat2std_xfm", "inputnode.anat2std_xfm"),
("outputnode.std2anat_xfm", "inputnode.std2anat_xfm"),
# Undefined if --fs-no-reconall, but this is safe
("outputnode.subjects_dir", "inputnode.subjects_dir"),
("outputnode.t1w2fsnative_xfm",
"inputnode.t1w2fsnative_xfm"),
("outputnode.fsnative2t1w_xfm",
"inputnode.fsnative2t1w_xfm"),
]),
(bids_info, dwi_preproc_wf, [("subject", "inputnode.subject_id")]),
])
# fmt: on
# Keep a handle to each workflow
dwi_preproc_list.append(dwi_preproc_wf)
if not fmap_estimators:
return workflow
# SDC Step 2: Manually add further estimators (e.g., fieldmap-less)
fmap_wf = init_fmap_preproc_wf(
debug=config.execution.debug,
estimators=fmap_estimators,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=str(output_dir),
subject=subject_id,
)
fmap_wf.__desc__ = f"""
*B<sub>0</sub>* fieldmap data preprocessing
: A total of {len(fmap_estimators)} fieldmaps were found available within the input
BIDS structure for this particular subject.
"""
# TODO: Requires nipreps/sdcflows#147
for dwi_preproc_wf in dwi_preproc_list:
# fmt: off
workflow.connect([
(fmap_wf, dwi_preproc_wf, [
("outputnode.fmap", "inputnode.fmap"),
("outputnode.fmap_ref", "inputnode.fmap_ref"),
("outputnode.fmap_coeff", "inputnode.fmap_coeff"),
("outputnode.fmap_mask", "inputnode.fmap_mask"),
("outputnode.fmap_id", "inputnode.fmap_id"),
]),
])
# fmt: on
# Overwrite ``out_path_base`` of sdcflows's DataSinks
for node in fmap_wf.list_node_names():
if node.split(".")[-1].startswith("ds_"):
fmap_wf.get_node(node).interface.out_path_base = "dmriprep"
# Step 3: Manually connect PEPOLAR
for estimator in fmap_estimators:
if estimator.method != fm.EstimatorType.PEPOLAR:
continue
suffices = set(s.suffix for s in estimator.sources)
if sorted(suffices) == ["epi"]:
getattr(fmap_wf.inputs, f"in_{estimator.bids_id}").in_data = [
str(s.path) for s in estimator.sources
]
getattr(fmap_wf.inputs, f"in_{estimator.bids_id}").metadata = [
s.metadata for s in estimator.sources
]
else:
raise NotImplementedError
# from niworkflows.interfaces.utility import KeySelect
# est_id = estimator.bids_id
# estim_select = pe.MapNode(
# KeySelect(fields=["metadata", "dwi_reference", "dwi_mask", "gradients_rasb",]),
# name=f"fmap_select_{est_id}",
# run_without_submitting=True,
# iterfields=["key"]
# )
# estim_select.inputs.key = [
# str(s.path) for s in estimator.sources if s.suffix in ("epi", "dwi", "sbref")
# ]
# # fmt:off
# workflow.connect([
# (referencenode, estim_select, [("dwi_file", "keys"),
# ("metadata", "metadata"),
# ("dwi_reference", "dwi_reference"),
# ("gradients_rasb", "gradients_rasb")]),
# ])
# # fmt:on
return workflow
def init_func_preproc_wf(bold_file, has_fieldmap=False):
"""
This workflow controls the functional preprocessing stages of *fMRIPrep*.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.tests import mock_config
from fmriprep import config
from fmriprep.workflows.bold.base import init_func_preproc_wf
with mock_config():
bold_file = config.execution.bids_dir / 'sub-01' / 'func' \
/ 'sub-01_task-mixedgamblestask_run-01_bold.nii.gz'
wf = init_func_preproc_wf(str(bold_file))
Parameters
----------
bold_file
BOLD series NIfTI file
has_fieldmap
Signals the workflow to use inputnode fieldmap files
Inputs
------
bold_file
BOLD series NIfTI file
t1w_preproc
Bias-corrected structural template image
t1w_mask
Mask of the skull-stripped template image
t1w_dseg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1w_asec
Segmentation of structural image, done with FreeSurfer.
t1w_aparc
Parcellation of structural image, done with FreeSurfer.
t1w_tpms
List of tissue probability maps in T1w space
template
List of templates to target
anat2std_xfm
List of transform files, collated with templates
std2anat_xfm
List of inverse transform files, collated with templates
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1w2fsnative_xfm
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
fsnative2t1w_xfm
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
bold_ref
BOLD reference file
bold_ref_xfm
Transform file in LTA format from bold to reference
n_dummy_scans
Number of nonsteady states at the beginning of the BOLD run
Outputs
-------
bold_t1
BOLD series, resampled to T1w space
bold_mask_t1
BOLD series mask in T1w space
bold_std
BOLD series, resampled to template space
bold_mask_std
BOLD series mask in template space
confounds
TSV of confounds
surfaces
BOLD series, resampled to FreeSurfer surfaces
aroma_noise_ics
Noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
bold_cifti
BOLD CIFTI image
cifti_variant
combination of target spaces for `bold_cifti`
See Also
--------
* :py:func:`~niworkflows.func.util.init_bold_reference_wf`
* :py:func:`~fmriprep.workflows.bold.stc.init_bold_stc_wf`
* :py:func:`~fmriprep.workflows.bold.hmc.init_bold_hmc_wf`
* :py:func:`~fmriprep.workflows.bold.t2s.init_bold_t2s_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_t1_trans_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_bold_confs_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_ica_aroma_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_std_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_preproc_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_surf_wf`
* :py:func:`~sdcflows.workflows.fmap.init_fmap_wf`
* :py:func:`~sdcflows.workflows.pepolar.init_pepolar_unwarp_wf`
* :py:func:`~sdcflows.workflows.phdiff.init_phdiff_wf`
* :py:func:`~sdcflows.workflows.syn.init_syn_sdc_wf`
* :py:func:`~sdcflows.workflows.unwarp.init_sdc_unwarp_wf`
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.utility import DictMerge
mem_gb = {'filesize': 1, 'resampled': 1, 'largemem': 1}
bold_tlen = 10
# Have some options handy
omp_nthreads = config.nipype.omp_nthreads
freesurfer = config.workflow.run_reconall
spaces = config.workflow.spaces
nibabies_dir = str(config.execution.nibabies_dir)
# Extract BIDS entities and metadata from BOLD file(s)
entities = extract_entities(bold_file)
layout = config.execution.layout
# Take first file as reference
ref_file = pop_file(bold_file)
metadata = layout.get_metadata(ref_file)
# get original image orientation
ref_orientation = get_img_orientation(ref_file)
echo_idxs = listify(entities.get("echo", []))
multiecho = len(echo_idxs) > 2
if len(echo_idxs) == 1:
config.loggers.workflow.warning(
f"Running a single echo <{ref_file}> from a seemingly multi-echo dataset."
)
bold_file = ref_file # Just in case - drop the list
if len(echo_idxs) == 2:
raise RuntimeError(
"Multi-echo processing requires at least three different echos (found two)."
)
if multiecho:
# Drop echo entity for future queries, have a boolean shorthand
entities.pop("echo", None)
# reorder echoes from shortest to largest
tes, bold_file = zip(*sorted([(layout.get_metadata(bf)["EchoTime"], bf)
for bf in bold_file]))
ref_file = bold_file[0] # Reset reference to be the shortest TE
if os.path.isfile(ref_file):
bold_tlen, mem_gb = _create_mem_gb(ref_file)
wf_name = _get_wf_name(ref_file)
config.loggers.workflow.debug(
f'Creating bold processing workflow for <{ref_file}> ({mem_gb["filesize"]:.2f} GB '
f'/ {bold_tlen} TRs). Memory resampled/largemem={mem_gb["resampled"]:.2f}'
f'/{mem_gb["largemem"]:.2f} GB.')
# Find associated sbref, if possible
entities['suffix'] = 'sbref'
entities['extension'] = ['.nii', '.nii.gz'] # Overwrite extensions
sbref_files = layout.get(return_type='file', **entities)
sbref_msg = f"No single-band-reference found for {os.path.basename(ref_file)}."
if sbref_files and 'sbref' in config.workflow.ignore:
sbref_msg = "Single-band reference file(s) found and ignored."
elif sbref_files:
sbref_msg = "Using single-band reference file(s) {}.".format(','.join(
[os.path.basename(sbf) for sbf in sbref_files]))
config.loggers.workflow.info(sbref_msg)
if has_fieldmap:
# Search for intended fieldmap
from pathlib import Path
import re
from sdcflows.fieldmaps import get_identifier
bold_rel = re.sub(r"^sub-[a-zA-Z0-9]*/", "",
str(Path(bold_file).relative_to(layout.root)))
estimator_key = get_identifier(bold_rel)
if not estimator_key:
has_fieldmap = False
config.loggers.workflow.critical(
f"None of the available B0 fieldmaps are associated to <{bold_rel}>"
)
# Short circuits: (True and True and (False or 'TooShort')) == 'TooShort'
run_stc = (bool(metadata.get("SliceTiming"))
and 'slicetiming' not in config.workflow.ignore
and (_get_series_len(ref_file) > 4 or "TooShort"))
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__postdesc__ = """\
All resamplings can be performed with *a single interpolation
step* by composing all the pertinent transformations (i.e. head-motion
transform matrices, susceptibility distortion correction when available,
and co-registrations to anatomical and output spaces).
Gridded (volumetric) resamplings were performed using `antsApplyTransforms` (ANTs),
configured with Lanczos interpolation to minimize the smoothing
effects of other kernels [@lanczos].
Non-gridded (surface) resamplings were performed using `mri_vol2surf`
(FreeSurfer).
"""
inputnode = pe.Node(
niu.IdentityInterface(fields=[
'bold_file',
# from smriprep
'anat_preproc',
'anat_brain',
'anat_mask',
'anat_dseg',
'anat_tpms',
'anat_aseg',
'anat_aparc',
'anat2std_xfm',
'std2anat_xfm',
'template',
# from bold reference workflow
'bold_ref',
'bold_ref_xfm',
'n_dummy_scans',
# from sdcflows (optional)
'fmap',
'fmap_ref',
'fmap_coeff',
'fmap_mask',
'fmap_id',
# if reconstructing with FreeSurfer (optional)
'anat2fsnative_xfm',
'fsnative2anat_xfm',
'subject_id',
'subjects_dir',
]),
name='inputnode')
inputnode.inputs.bold_file = bold_file
outputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_anat', 'bold_anat_ref', 'bold2anat_xfm', 'anat2bold_xfm',
'bold_mask_anat', 'bold_aseg_anat', 'bold_aparc_anat', 'bold_std',
'bold_std_ref', 'bold_mask_std', 'bold_aseg_std', 'bold_aparc_std',
'bold_native', 'bold_cifti', 'cifti_variant', 'cifti_metadata',
'cifti_density', 'surfaces', 'confounds', 'aroma_noise_ics',
'melodic_mix', 'nonaggr_denoised_file', 'confounds_metadata'
]),
name='outputnode')
# BOLD buffer: an identity used as a pointer to either the original BOLD
# or the STC'ed one for further use.
boldbuffer = pe.Node(niu.IdentityInterface(fields=['bold_file']),
name='boldbuffer')
summary = pe.Node(FunctionalSummary(
slice_timing=run_stc,
registration=('FSL', 'FreeSurfer')[freesurfer],
registration_dof=config.workflow.bold2t1w_dof,
registration_init=config.workflow.bold2t1w_init,
pe_direction=metadata.get("PhaseEncodingDirection"),
echo_idx=echo_idxs,
tr=metadata.get("RepetitionTime"),
orientation=ref_orientation),
name='summary',
mem_gb=config.DEFAULT_MEMORY_MIN_GB,
run_without_submitting=True)
summary.inputs.dummy_scans = config.workflow.dummy_scans
# TODO: SDC: make dynamic
summary.inputs.distortion_correction = 'None' if not has_fieldmap else 'TOPUP'
func_derivatives_wf = init_func_derivatives_wf(
bids_root=layout.root,
cifti_output=config.workflow.cifti_output,
freesurfer=freesurfer,
metadata=metadata,
output_dir=nibabies_dir,
spaces=spaces,
use_aroma=config.workflow.use_aroma,
debug=config.execution.debug,
)
workflow.connect([
(outputnode, func_derivatives_wf, [
('bold_anat', 'inputnode.bold_t1'),
('bold_anat_ref', 'inputnode.bold_t1_ref'),
('bold2anat_xfm', 'inputnode.bold2anat_xfm'),
('anat2bold_xfm', 'inputnode.anat2bold_xfm'),
('bold_aseg_anat', 'inputnode.bold_aseg_t1'),
('bold_aparc_anat', 'inputnode.bold_aparc_t1'),
('bold_mask_anat', 'inputnode.bold_mask_t1'),
('bold_native', 'inputnode.bold_native'),
('confounds', 'inputnode.confounds'),
('surfaces', 'inputnode.surf_files'),
('aroma_noise_ics', 'inputnode.aroma_noise_ics'),
('melodic_mix', 'inputnode.melodic_mix'),
('nonaggr_denoised_file', 'inputnode.nonaggr_denoised_file'),
('bold_cifti', 'inputnode.bold_cifti'),
('cifti_variant', 'inputnode.cifti_variant'),
('cifti_metadata', 'inputnode.cifti_metadata'),
('cifti_density', 'inputnode.cifti_density'),
('confounds_metadata', 'inputnode.confounds_metadata'),
('acompcor_masks', 'inputnode.acompcor_masks'),
('tcompcor_mask', 'inputnode.tcompcor_mask'),
]),
])
# Extract BOLD validation from init_bold_reference_wf
val_bold = pe.MapNode(
ValidateImage(),
name="val_bold",
mem_gb=config.DEFAULT_MEMORY_MIN_GB,
iterfield=["in_file"],
)
val_bold.inputs.in_file = listify(bold_file)
# Top-level BOLD splitter
bold_split = pe.Node(FSLSplit(dimension='t'),
name='bold_split',
mem_gb=mem_gb['filesize'] * 3)
# HMC on the BOLD
bold_hmc_wf = init_bold_hmc_wf(name='bold_hmc_wf',
mem_gb=mem_gb['filesize'],
omp_nthreads=omp_nthreads)
# calculate BOLD registration to T1w
bold_reg_wf = init_bold_reg_wf(
bold2t1w_dof=config.workflow.bold2t1w_dof,
bold2t1w_init=config.workflow.bold2t1w_init,
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
name='bold_reg_wf',
omp_nthreads=omp_nthreads,
sloppy=config.execution.sloppy,
use_bbr=config.workflow.use_bbr,
)
# apply BOLD registration to T1w
bold_t1_trans_wf = init_bold_t1_trans_wf(name='bold_t1_trans_wf',
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
if not has_fieldmap:
bold_t1_trans_wf.inputs.inputnode.fieldwarp = 'identity'
# get confounds
bold_confounds_wf = init_bold_confs_wf(
mem_gb=mem_gb['largemem'],
metadata=metadata,
freesurfer=freesurfer,
regressors_all_comps=config.workflow.regressors_all_comps,
regressors_fd_th=config.workflow.regressors_fd_th,
regressors_dvars_th=config.workflow.regressors_dvars_th,
name='bold_confounds_wf')
bold_confounds_wf.get_node('inputnode').inputs.t1_transform_flags = [False]
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_bold_trans_wf = init_bold_preproc_trans_wf(
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=not config.execution.low_mem,
use_fieldwarp=False, # TODO: Fieldwarp is already applied in new sdcflow
name='bold_bold_trans_wf')
bold_bold_trans_wf.inputs.inputnode.name_source = ref_file
# SLICE-TIME CORRECTION (or bypass) #############################################
if run_stc is True: # bool('TooShort') == True, so check True explicitly
bold_stc_wf = init_bold_stc_wf(name='bold_stc_wf', metadata=metadata)
workflow.connect([
(inputnode, bold_stc_wf, [('n_dummy_scans', 'inputnode.skip_vols')
]),
(bold_stc_wf, boldbuffer, [('outputnode.stc_file', 'bold_file')]),
])
if not multiecho:
workflow.connect([(val_bold, bold_stc_wf, [
(("out_file", pop_file), 'inputnode.bold_file')
])])
else: # for meepi, iterate through stc_wf for all workflows
meepi_echos = boldbuffer.clone(name='meepi_echos')
meepi_echos.iterables = ('bold_file', bold_file)
workflow.connect([(meepi_echos, bold_stc_wf,
[('bold_file', 'inputnode.bold_file')])])
elif not multiecho: # STC is too short or False
# bypass STC from original BOLD to the splitter through boldbuffer
workflow.connect([(val_bold, boldbuffer, [(("out_file", pop_file),
'bold_file')])])
else:
# for meepi, iterate over all meepi echos to boldbuffer
boldbuffer.iterables = ('bold_file', bold_file)
# MULTI-ECHO EPI DATA #############################################
if multiecho: # instantiate relevant interfaces, imports
from niworkflows.func.util import init_skullstrip_bold_wf
skullstrip_bold_wf = init_skullstrip_bold_wf(name='skullstrip_bold_wf')
split_opt_comb = bold_split.clone(name='split_opt_comb')
inputnode.inputs.bold_file = ref_file # Replace reference w first echo
join_echos = pe.JoinNode(
niu.IdentityInterface(
fields=['bold_files', 'skullstripped_bold_files']),
joinsource=('meepi_echos' if run_stc is True else 'boldbuffer'),
joinfield=['bold_files', 'skullstripped_bold_files'],
name='join_echos')
# create optimal combination, adaptive T2* map
bold_t2s_wf = init_bold_t2s_wf(echo_times=tes,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
name='bold_t2smap_wf')
# Mask BOLD reference image
final_boldref_masker = pe.Node(BrainExtraction(),
name='final_boldref_masker')
# MAIN WORKFLOW STRUCTURE #######################################################
workflow.connect([
# BOLD buffer has slice-time corrected if it was run, original otherwise
(boldbuffer, bold_split, [('bold_file', 'in_file')]),
# HMC
(inputnode, bold_hmc_wf, [('bold_ref', 'inputnode.raw_ref_image')]),
(inputnode, final_boldref_masker, [('bold_ref', 'in_file')]),
(val_bold, bold_hmc_wf, [(("out_file", pop_file),
'inputnode.bold_file')]),
(inputnode, summary, [('n_dummy_scans', 'algo_dummy_scans')]),
# EPI-T1 registration workflow
(
inputnode,
bold_reg_wf,
[
('anat_dseg', 'inputnode.t1w_dseg'),
# Undefined if --fs-no-reconall, but this is safe
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('fsnative2anat_xfm', 'inputnode.fsnative2t1w_xfm')
]),
(inputnode, bold_reg_wf, [('anat_brain', 'inputnode.t1w_brain')]),
(inputnode, bold_t1_trans_wf, [('bold_file', 'inputnode.name_source'),
('anat_mask', 'inputnode.t1w_mask'),
('anat_brain', 'inputnode.t1w_brain'),
('anat_aseg', 'inputnode.t1w_aseg'),
('anat_aparc', 'inputnode.t1w_aparc')]),
(bold_reg_wf, outputnode,
[('outputnode.itk_bold_to_t1', 'bold2anat_xfm'),
('outputnode.itk_t1_to_bold', 'anat2bold_xfm')]),
(bold_reg_wf, bold_t1_trans_wf, [('outputnode.itk_bold_to_t1',
'inputnode.itk_bold_to_t1')]),
(bold_t1_trans_wf, outputnode,
[('outputnode.bold_t1', 'bold_anat'),
('outputnode.bold_t1_ref', 'bold_anat_ref'),
('outputnode.bold_aseg_t1', 'bold_aseg_anat'),
('outputnode.bold_aparc_t1', 'bold_aparc_anat')]),
(bold_reg_wf, summary, [('outputnode.fallback', 'fallback')]),
# Connect bold_confounds_wf
(inputnode, bold_confounds_wf, [('anat_tpms', 'inputnode.t1w_tpms'),
('anat_mask', 'inputnode.t1w_mask')]),
(bold_hmc_wf, bold_confounds_wf,
[('outputnode.movpar_file', 'inputnode.movpar_file'),
('outputnode.rmsd_file', 'inputnode.rmsd_file')]),
(bold_reg_wf, bold_confounds_wf, [('outputnode.itk_t1_to_bold',
'inputnode.t1_bold_xform')]),
(inputnode, bold_confounds_wf, [('n_dummy_scans',
'inputnode.skip_vols')]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
('outputnode.confounds_metadata', 'confounds_metadata'),
('outputnode.acompcor_masks', 'acompcor_masks'),
('outputnode.tcompcor_mask', 'tcompcor_mask'),
]),
# Connect bold_bold_trans_wf
(bold_split, bold_bold_trans_wf, [('out_files', 'inputnode.bold_file')]
),
(bold_hmc_wf, bold_bold_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
# Summary
(outputnode, summary, [('confounds', 'confounds_file')]),
])
# for standard EPI data, pass along correct file
if not multiecho:
# TODO: Add SDC
workflow.connect([
(inputnode, func_derivatives_wf, [('bold_file',
'inputnode.source_file')]),
(bold_bold_trans_wf, bold_confounds_wf, [('outputnode.bold',
'inputnode.bold')]),
# (bold_bold_trans_wf, final_boldref_wf, [
# ('outputnode.bold', 'inputnode.bold_file')]),
(bold_split, bold_t1_trans_wf, [('out_files',
'inputnode.bold_split')]),
(bold_hmc_wf, bold_t1_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
# (bold_sdc_wf, bold_t1_trans_wf, [
# ('outputnode.out_warp', 'inputnode.fieldwarp')])
])
else: # for meepi, use optimal combination
workflow.connect([
# update name source for optimal combination
(inputnode, func_derivatives_wf,
[(('bold_file', combine_meepi_source), 'inputnode.source_file')]),
(bold_bold_trans_wf, join_echos, [('outputnode.bold', 'bold_files')
]),
# (join_echos, final_boldref_wf, [
# ('bold_files', 'inputnode.bold_file')]),
# TODO: Check with multi-echo data
(bold_bold_trans_wf, skullstrip_bold_wf, [('outputnode.bold',
'inputnode.in_file')]),
(skullstrip_bold_wf, join_echos,
[('outputnode.skull_stripped_file', 'skullstripped_bold_files')]),
(join_echos, bold_t2s_wf, [('skullstripped_bold_files',
'inputnode.bold_file')]),
(bold_t2s_wf, bold_confounds_wf, [('outputnode.bold',
'inputnode.bold')]),
(bold_t2s_wf, split_opt_comb, [('outputnode.bold', 'in_file')]),
(split_opt_comb, bold_t1_trans_wf, [('out_files',
'inputnode.bold_split')]),
])
# Already applied in bold_bold_trans_wf, which inputs to bold_t2s_wf
bold_t1_trans_wf.inputs.inputnode.fieldwarp = 'identity'
bold_t1_trans_wf.inputs.inputnode.hmc_xforms = 'identity'
# Map final BOLD mask into T1w space (if required)
nonstd_spaces = set(spaces.get_nonstandard())
if nonstd_spaces.intersection(('T1w', 'anat')):
from niworkflows.interfaces.fixes import (FixHeaderApplyTransforms as
ApplyTransforms)
boldmask_to_t1w = pe.Node(ApplyTransforms(interpolation='MultiLabel'),
name='boldmask_to_t1w',
mem_gb=0.1)
workflow.connect([
(bold_reg_wf, boldmask_to_t1w, [('outputnode.itk_bold_to_t1',
'transforms')]),
(bold_t1_trans_wf, boldmask_to_t1w, [('outputnode.bold_mask_t1',
'reference_image')]),
(boldmask_to_t1w, outputnode, [('output_image', 'bold_mask_anat')
]),
])
if nonstd_spaces.intersection(('func', 'run', 'bold', 'boldref', 'sbref')):
workflow.connect([
(inputnode, func_derivatives_wf, [
('bold_ref', 'inputnode.bold_native_ref'),
]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, outputnode,
[('outputnode.bold', 'bold_native')])
])
if spaces.get_spaces(nonstandard=False, dim=(3, )):
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_std_trans_wf = init_bold_std_trans_wf(
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
spaces=spaces,
name='bold_std_trans_wf',
use_compression=not config.execution.low_mem,
)
if not has_fieldmap:
bold_std_trans_wf.inputs.inputnode.fieldwarp = 'identity'
workflow.connect([
(inputnode, bold_std_trans_wf,
[('template', 'inputnode.templates'),
('anat2std_xfm', 'inputnode.anat2std_xfm'),
('bold_file', 'inputnode.name_source'),
('anat_aseg', 'inputnode.bold_aseg'),
('anat_aparc', 'inputnode.bold_aparc')]),
(bold_reg_wf, bold_std_trans_wf, [('outputnode.itk_bold_to_t1',
'inputnode.itk_bold_to_t1')]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_std', 'bold_std'),
('outputnode.bold_std_ref', 'bold_std_ref'),
('outputnode.bold_mask_std', 'bold_mask_std')]),
])
if freesurfer:
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('outputnode.bold_aseg_std', 'inputnode.bold_aseg_std'),
('outputnode.bold_aparc_std', 'inputnode.bold_aparc_std'),
]),
(bold_std_trans_wf, outputnode,
[('outputnode.bold_aseg_std', 'bold_aseg_std'),
('outputnode.bold_aparc_std', 'bold_aparc_std')]),
])
if not multiecho:
# TODO: Add SDC
workflow.connect([
(bold_split, bold_std_trans_wf, [('out_files',
'inputnode.bold_split')]),
# (bold_sdc_wf, bold_std_trans_wf, [
# ('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_hmc_wf, bold_std_trans_wf, [('outputnode.xforms',
'inputnode.hmc_xforms')]),
])
else:
workflow.connect([(split_opt_comb, bold_std_trans_wf,
[('out_files', 'inputnode.bold_split')])])
# Already applied in bold_bold_trans_wf, which inputs to bold_t2s_wf
bold_std_trans_wf.inputs.inputnode.fieldwarp = 'identity'
bold_std_trans_wf.inputs.inputnode.hmc_xforms = 'identity'
# func_derivatives_wf internally parametrizes over snapshotted spaces.
workflow.connect([
(bold_std_trans_wf, func_derivatives_wf, [
('outputnode.template', 'inputnode.template'),
('outputnode.spatial_reference',
'inputnode.spatial_reference'),
('outputnode.bold_std_ref', 'inputnode.bold_std_ref'),
('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
]),
])
if config.workflow.use_aroma: # ICA-AROMA workflow
from .confounds import init_ica_aroma_wf
ica_aroma_wf = init_ica_aroma_wf(
mem_gb=mem_gb['resampled'],
metadata=metadata,
omp_nthreads=omp_nthreads,
err_on_aroma_warn=config.workflow.aroma_err_on_warn,
aroma_melodic_dim=config.workflow.aroma_melodic_dim,
name='ica_aroma_wf')
join = pe.Node(niu.Function(output_names=["out_file"],
function=_to_join),
name='aroma_confounds')
mrg_conf_metadata = pe.Node(niu.Merge(2),
name='merge_confound_metadata',
run_without_submitting=True)
mrg_conf_metadata2 = pe.Node(DictMerge(),
name='merge_confound_metadata2',
run_without_submitting=True)
workflow.disconnect([
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_metadata', 'confounds_metadata'),
]),
])
workflow.connect([
(inputnode, ica_aroma_wf, [('bold_file',
'inputnode.name_source')]),
(bold_hmc_wf, ica_aroma_wf, [('outputnode.movpar_file',
'inputnode.movpar_file')]),
(inputnode, ica_aroma_wf, [('n_dummy_scans',
'inputnode.skip_vols')]),
(bold_confounds_wf, join, [('outputnode.confounds_file',
'in_file')]),
(bold_confounds_wf, mrg_conf_metadata,
[('outputnode.confounds_metadata', 'in1')]),
(ica_aroma_wf, join, [('outputnode.aroma_confounds',
'join_file')]),
(ica_aroma_wf, mrg_conf_metadata,
[('outputnode.aroma_metadata', 'in2')]),
(mrg_conf_metadata, mrg_conf_metadata2, [('out', 'in_dicts')]),
(ica_aroma_wf, outputnode,
[('outputnode.aroma_noise_ics', 'aroma_noise_ics'),
('outputnode.melodic_mix', 'melodic_mix'),
('outputnode.nonaggr_denoised_file', 'nonaggr_denoised_file')
]),
(join, outputnode, [('out_file', 'confounds')]),
(mrg_conf_metadata2, outputnode, [('out_dict',
'confounds_metadata')]),
(bold_std_trans_wf, ica_aroma_wf,
[('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.bold_mask_std', 'inputnode.bold_mask_std'),
('outputnode.spatial_reference',
'inputnode.spatial_reference')]),
])
# SURFACES ##################################################################################
# Freesurfer
freesurfer_spaces = spaces.get_fs_spaces()
if freesurfer and freesurfer_spaces:
config.loggers.workflow.debug(
'Creating BOLD surface-sampling workflow.')
bold_surf_wf = init_bold_surf_wf(
mem_gb=mem_gb['resampled'],
surface_spaces=freesurfer_spaces,
medial_surface_nan=config.workflow.medial_surface_nan,
name='bold_surf_wf')
workflow.connect([
(inputnode, bold_surf_wf,
[('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('anat2fsnative_xfm', 'inputnode.t1w2fsnative_xfm')]),
(bold_t1_trans_wf, bold_surf_wf, [('outputnode.bold_t1',
'inputnode.source_file')]),
(bold_surf_wf, outputnode, [('outputnode.surfaces', 'surfaces')]),
(bold_surf_wf, func_derivatives_wf, [('outputnode.target',
'inputnode.surf_refs')]),
])
# CIFTI output
if config.workflow.cifti_output:
from .resampling import init_bold_grayords_wf
bold_grayords_wf = init_bold_grayords_wf(
grayord_density=config.workflow.cifti_output,
mem_gb=mem_gb['resampled'],
repetition_time=metadata['RepetitionTime'])
workflow.connect([
(inputnode, bold_grayords_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bold_std_trans_wf, bold_grayords_wf,
[('outputnode.bold_std', 'inputnode.bold_std'),
('outputnode.spatial_reference',
'inputnode.spatial_reference')]),
(bold_surf_wf, bold_grayords_wf, [
('outputnode.surfaces', 'inputnode.surf_files'),
('outputnode.target', 'inputnode.surf_refs'),
]),
(bold_grayords_wf, outputnode,
[('outputnode.cifti_bold', 'bold_cifti'),
('outputnode.cifti_variant', 'cifti_variant'),
('outputnode.cifti_metadata', 'cifti_metadata'),
('outputnode.cifti_density', 'cifti_density')]),
])
if spaces.get_spaces(nonstandard=False, dim=(3, )):
if not config.workflow.cifti_output:
config.loggers.workflow.critical(
"The carpetplot requires CIFTI outputs")
else:
carpetplot_wf = init_carpetplot_wf(
mem_gb=mem_gb['resampled'],
metadata=metadata,
cifti_output=bool(config.workflow.cifti_output),
name='carpetplot_wf')
workflow.connect([
(bold_grayords_wf, carpetplot_wf, [('outputnode.cifti_bold',
'inputnode.cifti_bold')]),
(bold_confounds_wf, carpetplot_wf,
[('outputnode.confounds_file', 'inputnode.confounds_file')]),
])
# REPORTING ############################################################
ds_report_summary = pe.Node(DerivativesDataSink(
desc='summary', datatype="figures", dismiss_entities=("echo", )),
name='ds_report_summary',
run_without_submitting=True,
mem_gb=config.DEFAULT_MEMORY_MIN_GB)
ds_report_validation = pe.Node(DerivativesDataSink(
desc='validation', datatype="figures", dismiss_entities=("echo", )),
name='ds_report_validation',
run_without_submitting=True,
mem_gb=config.DEFAULT_MEMORY_MIN_GB)
workflow.connect([
(summary, ds_report_summary, [('out_report', 'in_file')]),
(val_bold, ds_report_validation, [(("out_report", pop_file), 'in_file')
]),
])
# Fill-in datasinks of reportlets seen so far
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_report'):
workflow.get_node(node).inputs.base_directory = nibabies_dir
workflow.get_node(node).inputs.source_file = ref_file
# Distortion correction
if not has_fieldmap:
# fmt: off
# Finalize workflow with fieldmap-less connections
workflow.connect([
(inputnode, final_boldref_masker, [('bold_ref', 'in_file')]),
(final_boldref_masker, bold_t1_trans_wf, [
('out_mask', 'inputnode.ref_bold_mask'),
('out_file', 'inputnode.ref_bold_brain'),
]),
(final_boldref_masker, bold_reg_wf, [
('out_file', 'inputnode.ref_bold_brain'),
]),
(final_boldref_masker, bold_confounds_wf,
[('out_mask', 'inputnode.bold_mask')]),
])
if nonstd_spaces.intersection(('T1w', 'anat')):
workflow.connect([
(final_boldref_masker, boldmask_to_t1w, [('out_mask',
'input_image')]),
])
# (final_boldref_wf, boldmask_to_t1w, [('outputnode.bold_mask', 'input_image')]),
# ])
if nonstd_spaces.intersection(
('func', 'run', 'bold', 'boldref', 'sbref')):
workflow.connect([
(final_boldref_masker, func_derivatives_wf,
[('out_file', 'inputnode.bold_native_ref'),
('out_mask', 'inputnode.bold_mask_native')]),
])
# (final_boldref_wf, func_derivatives_wf, [
# ('outputnode.ref_image', 'inputnode.bold_native_ref'),
# ('outputnode.bold_mask', 'inputnode.bold_mask_native')]),
# ])
if spaces.get_spaces(nonstandard=False, dim=(3, )):
workflow.connect([
(final_boldref_masker, bold_std_trans_wf,
[('out_mask', 'inputnode.bold_mask')]),
])
# (final_boldref_wf, bold_std_trans_wf, [
# ('outputnode.bold_mask', 'inputnode.bold_mask')]),
# ])
# fmt: on
return workflow
from niworkflows.interfaces.reportlets.registration import (
SimpleBeforeAfterRPT as SimpleBeforeAfter, )
from niworkflows.interfaces.utility import KeySelect
from sdcflows.workflows.apply.registration import init_coeff2epi_wf
from sdcflows.workflows.apply.correction import init_unwarp_wf
coeff2epi_wf = init_coeff2epi_wf(
debug="fieldmaps" in config.execution.debug,
omp_nthreads=config.nipype.omp_nthreads,
write_coeff=True,
)
unwarp_wf = init_unwarp_wf(debug="fieldmaps" in config.execution.debug,
omp_nthreads=config.nipype.omp_nthreads)
unwarp_wf.inputs.inputnode.metadata = layout.get_metadata(str(bold_file))
output_select = pe.Node(
KeySelect(fields=["fmap", "fmap_ref", "fmap_coeff", "fmap_mask"]),
name="output_select",
run_without_submitting=True,
)
output_select.inputs.key = estimator_key[0]
if len(estimator_key) > 1:
config.loggers.workflow.warning(
f"Several fieldmaps <{', '.join(estimator_key)}> are "
f"'IntendedFor' <{bold_file}>, using {estimator_key[0]}")
sdc_report = pe.Node(
SimpleBeforeAfter(before_label="Distorted", after_label="Corrected"),
name="sdc_report",
mem_gb=0.1,
)
ds_report_sdc = pe.Node(
DerivativesDataSink(base_directory=nibabies_dir,
desc="sdc",
suffix="bold",
datatype="figures",
dismiss_entities=("echo", )),
name="ds_report_sdc",
run_without_submitting=True,
)
unwarp_masker = pe.Node(BrainExtraction(), name='unwarp_masker')
# fmt: off
workflow.connect([
(inputnode, output_select, [("fmap", "fmap"), ("fmap_ref", "fmap_ref"),
("fmap_coeff", "fmap_coeff"),
("fmap_mask", "fmap_mask"),
("fmap_id", "keys")]),
(output_select, coeff2epi_wf, [("fmap_ref", "inputnode.fmap_ref"),
("fmap_coeff", "inputnode.fmap_coeff"),
("fmap_mask", "inputnode.fmap_mask")]),
(inputnode, coeff2epi_wf, [("bold_ref", "inputnode.target_ref")]),
(final_boldref_masker, coeff2epi_wf, [("out_file",
"inputnode.target_mask")]),
(inputnode, unwarp_wf, [("bold_ref", "inputnode.distorted")]),
(coeff2epi_wf, unwarp_wf, [("outputnode.fmap_coeff",
"inputnode.fmap_coeff")]),
(inputnode, sdc_report, [("bold_ref", "before")]),
(unwarp_wf, sdc_report, [("outputnode.corrected", "after"),
("outputnode.corrected_mask", "wm_seg")]),
(inputnode, ds_report_sdc, [("bold_file", "source_file")]),
(sdc_report, ds_report_sdc, [("out_report", "in_file")]),
# remaining workflow connections
(unwarp_wf, unwarp_masker, [('outputnode.corrected', 'in_file')]),
(unwarp_masker, bold_confounds_wf, [('out_mask', 'inputnode.bold_mask')
]),
(unwarp_masker, bold_t1_trans_wf,
[('out_mask', 'inputnode.ref_bold_mask'),
('out_file', 'inputnode.ref_bold_brain')]),
# (unwarp_masker, bold_bold_trans_wf, [
# ('out_mask', 'inputnode.bold_mask')]), # Not used within workflow
(unwarp_masker, bold_reg_wf, [('out_file', 'inputnode.ref_bold_brain')]
),
# TODO: Add distortion correction method to sdcflow outputs?
# (bold_sdc_wf, summary, [('outputnode.method', 'distortion_correction')]),
])
if nonstd_spaces.intersection(('T1w', 'anat')):
workflow.connect([
(unwarp_masker, boldmask_to_t1w, [('out_mask', 'input_image')]),
])
if nonstd_spaces.intersection(('func', 'run', 'bold', 'boldref', 'sbref')):
workflow.connect([
(unwarp_masker, func_derivatives_wf,
[('out_file', 'inputnode.bold_native_ref'),
('out_mask', 'inputnode.bold_mask_native')]),
])
if spaces.get_spaces(nonstandard=False, dim=(3, )):
workflow.connect([
(unwarp_masker, bold_std_trans_wf, [('out_mask',
'inputnode.bold_mask')]),
])
# fmt: on
# if not multiecho:
# (bold_sdc_wf, bold_t1_trans_wf, [
# ('outputnode.out_warp', 'inputnode.fieldwarp')])
# (bold_sdc_wf, bold_std_trans_wf, [
# ('outputnode.out_warp', 'inputnode.fieldwarp')]),
# ])
return workflow
def init_anat_preproc_wf(
bids_root, freesurfer, fs_spaces, hires, longitudinal,
omp_nthreads, output_dir, num_t1w, reportlets_dir,
skull_strip_template, template,
debug=False, name='anat_preproc_wf', skull_strip_fixed_seed=False):
r"""
This workflow controls the anatomical preprocessing stages of smriprep.
This includes:
- Creation of a structural template
- Skull-stripping and bias correction
- Tissue segmentation
- Normalization
- Surface reconstruction with FreeSurfer
.. workflow::
:graph2use: orig
:simple_form: yes
from smriprep.workflows.anatomical import init_anat_preproc_wf
wf = init_anat_preproc_wf(
bids_root='.',
freesurfer=True,
fs_spaces=['T1w', 'fsnative',
'template', 'fsaverage5'],
hires=True,
longitudinal=False,
omp_nthreads=1,
output_dir='.',
num_t1w=1,
reportlets_dir='.',
skull_strip_template='MNI152NLin2009cAsym',
template='MNI152NLin2009cAsym',
)
**Parameters**
bids_root : str
Path of the input BIDS dataset root
debug : bool
Enable debugging outputs
freesurfer : bool
Enable FreeSurfer surface reconstruction (increases runtime by 6h, at the very least)
fs_spaces : list
List of output spaces functional images are to be resampled to.
Some pipeline components will only be instantiated for some output spaces.
Valid spaces:
- T1w
- template
- fsnative
- fsaverage (or other pre-existing FreeSurfer templates)
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
longitudinal : bool
Create unbiased structural template, regardless of number of inputs
(may increase runtime)
name : str, optional
Workflow name (default: anat_preproc_wf)
omp_nthreads : int
Maximum number of threads an individual process may use
output_dir : str
Directory in which to save derivatives
reportlets_dir : str
Directory in which to save reportlets
skull_strip_fixed_seed : bool
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1 (default: ``False``)
skull_strip_template : str
Name of ANTs skull-stripping template ('MNI152NLin2009cAsym', 'OASIS30ANTs' or 'NKI')
template : str
Name of template targeted by ``template`` output space
**Inputs**
t1w
List of T1-weighted structural images
t2w
List of T2-weighted structural images
flair
List of FLAIR images
subjects_dir
FreeSurfer SUBJECTS_DIR
**Outputs**
t1_preproc
Bias-corrected structural template, defining T1w space
t1_brain
Skull-stripped ``t1_preproc``
t1_mask
Mask of the skull-stripped template image
t1_seg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1_tpms
List of tissue probability maps in T1w space
t1_2_mni
T1w template, normalized to MNI space
t1_2_mni_forward_transform
ANTs-compatible affine-and-warp transform file
t1_2_mni_reverse_transform
ANTs-compatible affine-and-warp transform file (inverse)
mni_mask
Mask of skull-stripped template, in MNI space
mni_seg
Segmentation, resampled into MNI space
mni_tpms
List of tissue probability maps in MNI space
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1_2_fsnative_forward_transform
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
t1_2_fsnative_reverse_transform
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
surfaces
GIFTI surfaces (gray/white boundary, midthickness, pial, inflated)
**Subworkflows**
* :py:func:`~niworkflows.anat.ants.init_brain_extraction_wf`
* :py:func:`~smriprep.workflows.surfaces.init_surface_recon_wf`
"""
if isinstance(template, list): # THIS SHOULD BE DELETED
template = template[0]
template_meta = get_metadata(template)
template_refs = ['@%s' % template.lower()]
if template_meta.get('RRID', None):
template_refs += ['RRID:%s' % template_meta['RRID']]
workflow = Workflow(name=name)
workflow.__postdesc__ = """\
Spatial normalization to the
*{template_name}* [{template_refs}]
was performed through nonlinear registration with `antsRegistration`
(ANTs {ants_ver}), using brain-extracted versions of both T1w volume
and template.
Brain tissue segmentation of cerebrospinal fluid (CSF),
white-matter (WM) and gray-matter (GM) was performed on
the brain-extracted T1w using `fast` [FSL {fsl_ver}, RRID:SCR_002823,
@fsl_fast].
""".format(
ants_ver=ANTsInfo.version() or '<ver>',
fsl_ver=fsl.FAST().version or '<ver>',
template_name=template_meta['Name'],
template_refs=', '.join(template_refs),
)
desc = """Anatomical data preprocessing
: """
desc += """\
A total of {num_t1w} T1-weighted (T1w) images were found within the input
BIDS dataset.
All of them were corrected for intensity non-uniformity (INU)
""" if num_t1w > 1 else """\
The T1-weighted (T1w) image was corrected for intensity non-uniformity (INU)
"""
desc += """\
with `N4BiasFieldCorrection` [@n4], distributed with ANTs {ants_ver} \
[@ants, RRID:SCR_004757]"""
desc += '.\n' if num_t1w > 1 else ", and used as T1w-reference throughout the workflow.\n"
desc += """\
The T1w-reference was then skull-stripped with a *Nipype* implementation of
the `antsBrainExtraction.sh` workflow (from ANTs), using {skullstrip_tpl}
as target template.
""".format(skullstrip_tpl=skull_strip_template)
workflow.__desc__ = desc.format(
num_t1w=num_t1w,
ants_ver=ANTsInfo.version() or '<ver>'
)
inputnode = pe.Node(
niu.IdentityInterface(fields=['t1w', 't2w', 'roi', 'flair', 'subjects_dir', 'subject_id']),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(
fields=['t1_preproc', 't1_brain', 't1_mask', 't1_seg', 't1_tpms',
't1_2_mni', 't1_2_mni_forward_transform', 't1_2_mni_reverse_transform',
'mni_mask', 'mni_seg', 'mni_tpms',
'template_transforms',
'subjects_dir', 'subject_id', 't1_2_fsnative_forward_transform',
't1_2_fsnative_reverse_transform', 'surfaces', 't1_aseg', 't1_aparc']),
name='outputnode')
buffernode = pe.Node(niu.IdentityInterface(
fields=['t1_brain', 't1_mask']), name='buffernode')
anat_template_wf = init_anat_template_wf(longitudinal=longitudinal, omp_nthreads=omp_nthreads,
num_t1w=num_t1w)
# 3. Skull-stripping
# Bias field correction is handled in skull strip workflows.
brain_extraction_wf = init_brain_extraction_wf(
in_template=skull_strip_template,
atropos_use_random_seed=not skull_strip_fixed_seed,
omp_nthreads=omp_nthreads,
normalization_quality='precise' if not debug else 'testing')
workflow.connect([
(inputnode, anat_template_wf, [('t1w', 'inputnode.t1w')]),
(anat_template_wf, brain_extraction_wf, [
('outputnode.t1_template', 'inputnode.in_files')]),
(brain_extraction_wf, outputnode, [
('outputnode.bias_corrected', 't1_preproc')]),
(anat_template_wf, outputnode, [
('outputnode.template_transforms', 't1_template_transforms')]),
(buffernode, outputnode, [('t1_brain', 't1_brain'),
('t1_mask', 't1_mask')]),
])
# 4. Surface reconstruction
if freesurfer:
surface_recon_wf = init_surface_recon_wf(name='surface_recon_wf',
omp_nthreads=omp_nthreads, hires=hires)
applyrefined = pe.Node(fsl.ApplyMask(), name='applyrefined')
workflow.connect([
(inputnode, surface_recon_wf, [
('t2w', 'inputnode.t2w'),
('flair', 'inputnode.flair'),
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id')]),
(anat_template_wf, surface_recon_wf, [('outputnode.t1_template', 'inputnode.t1w')]),
(brain_extraction_wf, surface_recon_wf, [
(('outputnode.out_file', _pop), 'inputnode.skullstripped_t1'),
('outputnode.out_segm', 'inputnode.ants_segs'),
(('outputnode.bias_corrected', _pop), 'inputnode.corrected_t1')]),
(brain_extraction_wf, applyrefined, [
(('outputnode.bias_corrected', _pop), 'in_file')]),
(surface_recon_wf, applyrefined, [
('outputnode.out_brainmask', 'mask_file')]),
(surface_recon_wf, outputnode, [
('outputnode.subjects_dir', 'subjects_dir'),
('outputnode.subject_id', 'subject_id'),
('outputnode.t1_2_fsnative_forward_transform', 't1_2_fsnative_forward_transform'),
('outputnode.t1_2_fsnative_reverse_transform', 't1_2_fsnative_reverse_transform'),
('outputnode.surfaces', 'surfaces'),
('outputnode.out_aseg', 't1_aseg'),
('outputnode.out_aparc', 't1_aparc')]),
(applyrefined, buffernode, [('out_file', 't1_brain')]),
(surface_recon_wf, buffernode, [
('outputnode.out_brainmask', 't1_mask')]),
])
else:
workflow.connect([
(brain_extraction_wf, buffernode, [
(('outputnode.out_file', _pop), 't1_brain'),
('outputnode.out_mask', 't1_mask')]),
])
# 5. Segmentation
t1_seg = pe.Node(fsl.FAST(segments=True, no_bias=True, probability_maps=True),
name='t1_seg', mem_gb=3)
workflow.connect([
(buffernode, t1_seg, [('t1_brain', 'in_files')]),
(t1_seg, outputnode, [('tissue_class_map', 't1_seg'),
('probability_maps', 't1_tpms')]),
])
# 6. Spatial normalization (T1w to MNI registration)
t1_2_mni = pe.Node(
RobustMNINormalizationRPT(
float=True,
generate_report=True,
flavor='testing' if debug else 'precise',
),
name='t1_2_mni',
n_procs=omp_nthreads,
mem_gb=2
)
# Resample the brain mask and the tissue probability maps into mni space
mni_mask = pe.Node(
ApplyTransforms(dimension=3, default_value=0, float=True,
interpolation='MultiLabel'),
name='mni_mask'
)
mni_seg = pe.Node(
ApplyTransforms(dimension=3, default_value=0, float=True,
interpolation='MultiLabel'),
name='mni_seg'
)
mni_tpms = pe.MapNode(
ApplyTransforms(dimension=3, default_value=0, float=True,
interpolation='Linear'),
iterfield=['input_image'],
name='mni_tpms'
)
# TODO isolate the spatial normalization workflow #############
ref_img = str(get_template(template, resolution=1, desc=None, suffix='T1w',
extensions=['.nii', '.nii.gz']))
t1_2_mni.inputs.template = template
mni_mask.inputs.reference_image = ref_img
mni_seg.inputs.reference_image = ref_img
mni_tpms.inputs.reference_image = ref_img
workflow.connect([
(inputnode, t1_2_mni, [('roi', 'lesion_mask')]),
(brain_extraction_wf, t1_2_mni, [
(('outputnode.bias_corrected', _pop), 'moving_image')]),
(buffernode, t1_2_mni, [('t1_mask', 'moving_mask')]),
(buffernode, mni_mask, [('t1_mask', 'input_image')]),
(t1_2_mni, mni_mask, [('composite_transform', 'transforms')]),
(t1_seg, mni_seg, [('tissue_class_map', 'input_image')]),
(t1_2_mni, mni_seg, [('composite_transform', 'transforms')]),
(t1_seg, mni_tpms, [('probability_maps', 'input_image')]),
(t1_2_mni, mni_tpms, [('composite_transform', 'transforms')]),
(t1_2_mni, outputnode, [
('warped_image', 't1_2_mni'),
('composite_transform', 't1_2_mni_forward_transform'),
('inverse_composite_transform', 't1_2_mni_reverse_transform')]),
(mni_mask, outputnode, [('output_image', 'mni_mask')]),
(mni_seg, outputnode, [('output_image', 'mni_seg')]),
(mni_tpms, outputnode, [('output_image', 'mni_tpms')]),
])
# spatial normalization ends here ###############################
seg_rpt = pe.Node(ROIsPlot(colors=['magenta', 'b'], levels=[1.5, 2.5]),
name='seg_rpt')
anat_reports_wf = init_anat_reports_wf(
reportlets_dir=reportlets_dir, template=template,
freesurfer=freesurfer)
workflow.connect([
(inputnode, anat_reports_wf, [
(('t1w', fix_multi_T1w_source_name), 'inputnode.source_file')]),
(anat_template_wf, anat_reports_wf, [
('outputnode.out_report', 'inputnode.t1_conform_report')]),
(anat_template_wf, seg_rpt, [
('outputnode.t1_template', 'in_file')]),
(t1_seg, seg_rpt, [('tissue_class_map', 'in_rois')]),
(outputnode, seg_rpt, [('t1_mask', 'in_mask')]),
(seg_rpt, anat_reports_wf, [('out_report', 'inputnode.seg_report')]),
(t1_2_mni, anat_reports_wf, [('out_report', 'inputnode.t1_2_mni_report')]),
])
if freesurfer:
workflow.connect([
(surface_recon_wf, anat_reports_wf, [
('outputnode.out_report', 'inputnode.recon_report')]),
])
anat_derivatives_wf = init_anat_derivatives_wf(
bids_root=bids_root,
freesurfer=freesurfer,
output_dir=output_dir,
template=template,
)
workflow.connect([
(anat_template_wf, anat_derivatives_wf, [
('outputnode.t1w_valid_list', 'inputnode.source_files')]),
(outputnode, anat_derivatives_wf, [
('t1_template_transforms', 'inputnode.t1_template_transforms'),
('t1_preproc', 'inputnode.t1_preproc'),
('t1_mask', 'inputnode.t1_mask'),
('t1_seg', 'inputnode.t1_seg'),
('t1_tpms', 'inputnode.t1_tpms'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform'),
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform'),
('t1_2_mni', 'inputnode.t1_2_mni'),
('mni_mask', 'inputnode.mni_mask'),
('mni_seg', 'inputnode.mni_seg'),
('mni_tpms', 'inputnode.mni_tpms'),
('t1_2_fsnative_forward_transform', 'inputnode.t1_2_fsnative_forward_transform'),
('surfaces', 'inputnode.surfaces'),
]),
])
if freesurfer:
workflow.connect([
(surface_recon_wf, anat_derivatives_wf, [
('outputnode.out_aseg', 'inputnode.t1_fs_aseg'),
('outputnode.out_aparc', 'inputnode.t1_fs_aparc'),
]),
])
return workflow
def init_ica_aroma_wf(template, metadata, mem_gb, omp_nthreads,
name='ica_aroma_wf',
susan_fwhm=6.0,
ignore_aroma_err=False,
aroma_melodic_dim=-200,
use_fieldwarp=True):
"""
This workflow wraps `ICA-AROMA`_ to identify and remove motion-related
independent components from a BOLD time series.
The following steps are performed:
#. Remove non-steady state volumes from the bold series.
#. Smooth data using FSL `susan`, with a kernel width FWHM=6.0mm.
#. Run FSL `melodic` outside of ICA-AROMA to generate the report
#. Run ICA-AROMA
#. Aggregate identified motion components (aggressive) to TSV
#. Return ``classified_motion_ICs`` and ``melodic_mix`` for user to complete
non-aggressive denoising in T1w space
#. Calculate ICA-AROMA-identified noise components
(columns named ``AROMAAggrCompXX``)
Additionally, non-aggressive denoising is performed on the BOLD series
resampled into MNI space.
There is a current discussion on whether other confounds should be extracted
before or after denoising `here <http://nbviewer.jupyter.org/github/poldracklab/\
fmriprep-notebooks/blob/922e436429b879271fa13e76767a6e73443e74d9/issue-817_\
aroma_confounds.ipynb>`__.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.bold.confounds import init_ica_aroma_wf
wf = init_ica_aroma_wf(template='MNI152NLin2009cAsym',
metadata={'RepetitionTime': 1.0},
mem_gb=3,
omp_nthreads=1)
**Parameters**
template : str
Spatial normalization template used as target when that
registration step was previously calculated with
:py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`.
The template must be one of the MNI templates (fMRIPrep uses
``MNI152NLin2009cAsym`` by default).
metadata : dict
BIDS metadata for BOLD file
mem_gb : float
Size of BOLD file in GB
omp_nthreads : int
Maximum number of threads an individual process may use
name : str
Name of workflow (default: ``bold_mni_trans_wf``)
susan_fwhm : float
Kernel width (FWHM in mm) for the smoothing step with
FSL ``susan`` (default: 6.0mm)
use_fieldwarp : bool
Include SDC warp in single-shot transform from BOLD to MNI
ignore_aroma_err : bool
Do not fail on ICA-AROMA errors
aroma_melodic_dim: int
Set the dimensionality of the MELODIC ICA decomposition.
Negative numbers set a maximum on automatic dimensionality estimation.
Positive numbers set an exact number of components to extract.
(default: -200, i.e., estimate <=200 components)
**Inputs**
itk_bold_to_t1
Affine transform from ``ref_bold_brain`` to T1 space (ITK format)
t1_2_mni_forward_transform
ANTs-compatible affine-and-warp transform file
name_source
BOLD series NIfTI file
Used to recover original information lost during processing
skip_vols
number of non steady state volumes
bold_split
Individual 3D BOLD volumes, not motion corrected
bold_mask
BOLD series mask in template space
hmc_xforms
List of affine transforms aligning each volume to ``ref_image`` in ITK format
fieldwarp
a :abbr:`DFM (displacements field map)` in ITK format
movpar_file
SPM-formatted motion parameters file
**Outputs**
aroma_confounds
TSV of confounds identified as noise by ICA-AROMA
aroma_noise_ics
CSV of noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
nonaggr_denoised_file
BOLD series with non-aggressive ICA-AROMA denoising applied
.. _ICA-AROMA: https://github.com/maartenmennes/ICA-AROMA
"""
workflow = Workflow(name=name)
workflow.__postdesc__ = """\
Automatic removal of motion artifacts using independent component analysis
[ICA-AROMA, @aroma] was performed on the *preprocessed BOLD on MNI space*
time-series after removal of non-steady state volumes and spatial smoothing
with an isotropic, Gaussian kernel of 6mm FWHM (full-width half-maximum).
Corresponding "non-aggresively" denoised runs were produced after such
smoothing.
Additionally, the "aggressive" noise-regressors were collected and placed
in the corresponding confounds file.
"""
inputnode = pe.Node(niu.IdentityInterface(
fields=[
'itk_bold_to_t1',
't1_2_mni_forward_transform',
'name_source',
'skip_vols',
'bold_split',
'bold_mask',
'hmc_xforms',
'fieldwarp',
'movpar_file']), name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(
fields=['aroma_confounds', 'aroma_noise_ics', 'melodic_mix',
'nonaggr_denoised_file']), name='outputnode')
bold_mni_trans_wf = init_bold_mni_trans_wf(
template=template,
freesurfer=False,
mem_gb=mem_gb,
omp_nthreads=omp_nthreads,
template_out_grid=str(
get_template('MNI152Lin') / 'tpl-MNI152Lin_space-MNI_res-02_T1w.nii.gz'),
use_compression=False,
use_fieldwarp=use_fieldwarp,
name='bold_mni_trans_wf'
)
bold_mni_trans_wf.__desc__ = None
rm_non_steady_state = pe.Node(niu.Function(function=_remove_volumes,
output_names=['bold_cut']),
name='rm_nonsteady')
calc_median_val = pe.Node(fsl.ImageStats(op_string='-k %s -p 50'), name='calc_median_val')
calc_bold_mean = pe.Node(fsl.MeanImage(), name='calc_bold_mean')
def _getusans_func(image, thresh):
return [tuple([image, thresh])]
getusans = pe.Node(niu.Function(function=_getusans_func, output_names=['usans']),
name='getusans', mem_gb=0.01)
smooth = pe.Node(fsl.SUSAN(fwhm=susan_fwhm), name='smooth')
# melodic node
melodic = pe.Node(fsl.MELODIC(
no_bet=True, tr_sec=float(metadata['RepetitionTime']), mm_thresh=0.5, out_stats=True,
dim=aroma_melodic_dim), name="melodic")
# ica_aroma node
ica_aroma = pe.Node(ICA_AROMARPT(
denoise_type='nonaggr', generate_report=True, TR=metadata['RepetitionTime']),
name='ica_aroma')
add_non_steady_state = pe.Node(niu.Function(function=_add_volumes,
output_names=['bold_add']),
name='add_nonsteady')
# extract the confound ICs from the results
ica_aroma_confound_extraction = pe.Node(ICAConfounds(ignore_aroma_err=ignore_aroma_err),
name='ica_aroma_confound_extraction')
ds_report_ica_aroma = pe.Node(
DerivativesDataSink(suffix='ica_aroma'),
name='ds_report_ica_aroma', run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
def _getbtthresh(medianval):
return 0.75 * medianval
# connect the nodes
workflow.connect([
(inputnode, bold_mni_trans_wf, [
('name_source', 'inputnode.name_source'),
('bold_split', 'inputnode.bold_split'),
('bold_mask', 'inputnode.bold_mask'),
('hmc_xforms', 'inputnode.hmc_xforms'),
('itk_bold_to_t1', 'inputnode.itk_bold_to_t1'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform'),
('fieldwarp', 'inputnode.fieldwarp')]),
(inputnode, ica_aroma, [('movpar_file', 'motion_parameters')]),
(inputnode, rm_non_steady_state, [
('skip_vols', 'skip_vols')]),
(bold_mni_trans_wf, rm_non_steady_state, [
('outputnode.bold_mni', 'bold_file')]),
(bold_mni_trans_wf, calc_median_val, [
('outputnode.bold_mask_mni', 'mask_file')]),
(rm_non_steady_state, calc_median_val, [
('bold_cut', 'in_file')]),
(rm_non_steady_state, calc_bold_mean, [
('bold_cut', 'in_file')]),
(calc_bold_mean, getusans, [('out_file', 'image')]),
(calc_median_val, getusans, [('out_stat', 'thresh')]),
# Connect input nodes to complete smoothing
(rm_non_steady_state, smooth, [
('bold_cut', 'in_file')]),
(getusans, smooth, [('usans', 'usans')]),
(calc_median_val, smooth, [(('out_stat', _getbtthresh), 'brightness_threshold')]),
# connect smooth to melodic
(smooth, melodic, [('smoothed_file', 'in_files')]),
(bold_mni_trans_wf, melodic, [
('outputnode.bold_mask_mni', 'mask')]),
# connect nodes to ICA-AROMA
(smooth, ica_aroma, [('smoothed_file', 'in_file')]),
(bold_mni_trans_wf, ica_aroma, [
('outputnode.bold_mask_mni', 'report_mask'),
('outputnode.bold_mask_mni', 'mask')]),
(melodic, ica_aroma, [('out_dir', 'melodic_dir')]),
# generate tsvs from ICA-AROMA
(ica_aroma, ica_aroma_confound_extraction, [('out_dir', 'in_directory')]),
(inputnode, ica_aroma_confound_extraction, [
('skip_vols', 'skip_vols')]),
# output for processing and reporting
(ica_aroma_confound_extraction, outputnode, [('aroma_confounds', 'aroma_confounds'),
('aroma_noise_ics', 'aroma_noise_ics'),
('melodic_mix', 'melodic_mix')]),
# TODO change melodic report to reflect noise and non-noise components
(ica_aroma, add_non_steady_state, [
('nonaggr_denoised_file', 'bold_cut_file')]),
(bold_mni_trans_wf, add_non_steady_state, [
('outputnode.bold_mni', 'bold_file')]),
(inputnode, add_non_steady_state, [
('skip_vols', 'skip_vols')]),
(add_non_steady_state, outputnode, [('bold_add', 'nonaggr_denoised_file')]),
(ica_aroma, ds_report_ica_aroma, [('out_report', 'in_file')]),
])
return workflow
def init_nibetaseries_participant_wf(
estimator,
atlas_img,
atlas_lut,
bids_dir,
database_path,
derivatives_pipeline_dir,
exclude_description_label,
fir_delays,
hrf_model,
high_pass,
norm_betas,
output_dir,
return_residuals,
run_label,
selected_confounds,
session_label,
signal_scaling,
smoothing_kernel,
space_label,
subject_list,
task_label,
description_label,
work_dir,
):
"""
This workflow organizes the execution of NiBetaSeries, with a sub-workflow for
each subject.
Parameters
----------
atlas_img : str
Path to input atlas nifti
atlas_lut : str
Path to input atlas lookup table (tsv)
bids_dir : str
Root directory of BIDS dataset
database_path : str
Path to a BIDS database
derivatives_pipeline_dir : str
Root directory of the derivatives pipeline
exclude_description_label : str or None
Exclude bold series containing this description label
fir_delays : list or None
FIR delays (in scans)
hrf_model : str
The model that represents the shape of the hemodynamic response function
high_pass : float
High pass filter to apply to bold (in Hertz).
Reminder - frequencies _higher_ than this number are kept.
norm_betas : Bool
If True, beta estimates are divided by the square root of their variance
output_dir : str
Directory where derivatives are saved
return_residuals : bool
Output the residuals from the betaseries model into the
derivatives directory
run_label : str or None
Include bold series containing this run label
selected_confounds : list
List of confounds to be included in regression
signal_scaling : False or 0
Whether (0) or not (False) to scale each voxel's timeseries
session_label : str or None
Include bold series containing this session label
smoothing_kernel : float or None
The smoothing kernel to be applied to the bold series before beta estimation
space_label : str or None
Include bold series containing this space label
subject_list : list
List of subject labels
task_label : str or None
Include bold series containing this task label
description_label : str or None
Include bold series containing this description label
work_dir : str
Directory in which to store workflow execution state and temporary files
"""
# setup workflow
nibetaseries_participant_wf = Workflow(name='nibetaseries_participant_wf')
nibetaseries_participant_wf.base_dir = os.path.join(
work_dir, 'NiBetaSeries_work')
os.makedirs(nibetaseries_participant_wf.base_dir, exist_ok=True)
nibetaseries_participant_wf.__desc__ = """
Results included in this manuscript come from modeling
performed using *NiBetaSeries* {nibs_ver} [@Kent2018],
which is based on *Nipype* {nipype_ver} [@Gorgolewski2011; @Gorgolewski2018].
""".format(nibs_ver=get_versions()['version'], nipype_ver=nipype_ver)
nibetaseries_participant_wf.__postdesc__ = """
### Software Dependencies
Additional libraries used in the NiBetaSeries workflow include
*Pybids* {pybids_ver} [@Yarkoni2019], *Niworkflows* {niworkflows_ver},
*Nibabel* {nibabel_ver}, *Pandas* {pandas_ver} [@McKinney2010], and
*Numpy* {numpy_ver} [@VanDerWalt2011; @Oliphant2006].
### Copyright Waiver
The above boilerplate text was automatically generated by NiBetaSeries
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(pybids_ver=pybids_ver,
niworkflows_ver=niworkflows_ver,
nibabel_ver=nibabel_ver,
pandas_ver=pandas_ver,
numpy_ver=numpy_ver)
# Go ahead and initialize the layout database
if database_path is None:
database_path = os.path.join(work_dir, 'dbcache')
reset_database = True
else:
reset_database = False
# reading in derivatives and bids inputs as queryable database like objects
layout = BIDSLayout(bids_dir,
derivatives=derivatives_pipeline_dir,
index_metadata=False,
database_file=database_path,
reset_database=reset_database)
# only index bold file metadata
if reset_database:
indexer = BIDSLayoutIndexerPatch(layout)
metadata_filter = {
'extension': ['nii', 'nii.gz', 'json'],
'suffix': 'bold',
}
indexer.index_metadata(**metadata_filter)
for subject_label in subject_list:
# collect the necessary inputs for both collect data
subject_data = collect_data(layout,
subject_label,
task=task_label,
run=run_label,
ses=session_label,
space=space_label,
description=description_label)
# collect files to be associated with each preproc
brainmask_list = [d['brainmask'] for d in subject_data]
confound_tsv_list = [d['confounds'] for d in subject_data]
events_tsv_list = [d['events'] for d in subject_data]
preproc_img_list = [d['preproc'] for d in subject_data]
bold_metadata_list = [d['metadata'] for d in subject_data]
single_subject_wf = init_single_subject_wf(
estimator=estimator,
atlas_img=atlas_img,
atlas_lut=atlas_lut,
bold_metadata_list=bold_metadata_list,
brainmask_list=brainmask_list,
confound_tsv_list=confound_tsv_list,
events_tsv_list=events_tsv_list,
fir_delays=fir_delays,
hrf_model=hrf_model,
high_pass=high_pass,
name='single_subject' + subject_label + '_wf',
norm_betas=norm_betas,
output_dir=output_dir,
preproc_img_list=preproc_img_list,
return_residuals=return_residuals,
selected_confounds=selected_confounds,
signal_scaling=signal_scaling,
smoothing_kernel=smoothing_kernel,
)
single_subject_wf.config['execution']['crashdump_dir'] = (os.path.join(
output_dir, "sub-" + subject_label, 'log'))
for node in single_subject_wf._get_all_nodes():
node.config = deepcopy(single_subject_wf.config)
nibetaseries_participant_wf.add_nodes([single_subject_wf])
return nibetaseries_participant_wf
def init_func_preproc_wf(bold_file, ignore, freesurfer,
use_bbr, t2s_coreg, bold2t1w_dof, reportlets_dir,
output_spaces, template, output_dir, omp_nthreads,
fmap_bspline, fmap_demean, use_syn, force_syn,
use_aroma, ignore_aroma_err, aroma_melodic_dim,
medial_surface_nan, cifti_output,
debug, low_mem, template_out_grid,
layout=None, num_bold=1):
"""
This workflow controls the functional preprocessing stages of FMRIPREP.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.bold import init_func_preproc_wf
wf = init_func_preproc_wf('/completely/made/up/path/sub-01_task-nback_bold.nii.gz',
omp_nthreads=1,
ignore=[],
freesurfer=True,
reportlets_dir='.',
output_dir='.',
template='MNI152NLin2009cAsym',
output_spaces=['T1w', 'fsnative',
'template', 'fsaverage5'],
debug=False,
use_bbr=True,
t2s_coreg=False,
bold2t1w_dof=9,
fmap_bspline=True,
fmap_demean=True,
use_syn=True,
force_syn=True,
low_mem=False,
template_out_grid='native',
medial_surface_nan=False,
cifti_output=False,
use_aroma=False,
ignore_aroma_err=False,
aroma_melodic_dim=-200,
num_bold=1)
**Parameters**
bold_file : str
BOLD series NIfTI file
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
freesurfer : bool
Enable FreeSurfer functional registration (bbregister) and resampling
BOLD series to FreeSurfer surface meshes.
use_bbr : bool or None
Enable/disable boundary-based registration refinement.
If ``None``, test BBR result for distortion before accepting.
When using ``t2s_coreg``, BBR will be enabled by default unless
explicitly specified otherwise.
t2s_coreg : bool
For multiecho EPI, use the calculated T2*-map for T2*-driven coregistration
bold2t1w_dof : 6, 9 or 12
Degrees-of-freedom for BOLD-T1w registration
reportlets_dir : str
Directory in which to save reportlets
output_spaces : list
List of output spaces functional images are to be resampled to.
Some parts of pipeline will only be instantiated for some output spaces.
Valid spaces:
- T1w
- template
- fsnative
- fsaverage (or other pre-existing FreeSurfer templates)
template : str
Name of template targeted by ``template`` output space
output_dir : str
Directory in which to save derivatives
omp_nthreads : int
Maximum number of threads an individual process may use
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
force_syn : bool
**Temporary**: Always run SyN-based SDC
use_aroma : bool
Perform ICA-AROMA on MNI-resampled functional series
ignore_aroma_err : bool
Do not fail on ICA-AROMA errors
medial_surface_nan : bool
Replace medial wall values with NaNs on functional GIFTI files
cifti_output : bool
Generate bold CIFTI file in output spaces
debug : bool
Enable debugging outputs
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
template_out_grid : str
Keyword ('native', '1mm' or '2mm') or path of custom reference
image for normalization
layout : BIDSLayout
BIDSLayout structure to enable metadata retrieval
num_bold : int
Total number of BOLD files that have been set for preprocessing
(default is 1)
**Inputs**
bold_file
BOLD series NIfTI file
t1_preproc
Bias-corrected structural template image
t1_brain
Skull-stripped ``t1_preproc``
t1_mask
Mask of the skull-stripped template image
t1_seg
Segmentation of preprocessed structural image, including
gray-matter (GM), white-matter (WM) and cerebrospinal fluid (CSF)
t1_tpms
List of tissue probability maps in T1w space
t1_2_mni_forward_transform
ANTs-compatible affine-and-warp transform file
t1_2_mni_reverse_transform
ANTs-compatible affine-and-warp transform file (inverse)
subjects_dir
FreeSurfer SUBJECTS_DIR
subject_id
FreeSurfer subject ID
t1_2_fsnative_forward_transform
LTA-style affine matrix translating from T1w to FreeSurfer-conformed subject space
t1_2_fsnative_reverse_transform
LTA-style affine matrix translating from FreeSurfer-conformed subject space to T1w
**Outputs**
bold_t1
BOLD series, resampled to T1w space
bold_mask_t1
BOLD series mask in T1w space
bold_mni
BOLD series, resampled to template space
bold_mask_mni
BOLD series mask in template space
confounds
TSV of confounds
surfaces
BOLD series, resampled to FreeSurfer surfaces
aroma_noise_ics
Noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
bold_cifti
BOLD CIFTI image
cifti_variant
combination of target spaces for `bold_cifti`
**Subworkflows**
* :py:func:`~fmriprep.workflows.bold.util.init_bold_reference_wf`
* :py:func:`~fmriprep.workflows.bold.stc.init_bold_stc_wf`
* :py:func:`~fmriprep.workflows.bold.hmc.init_bold_hmc_wf`
* :py:func:`~fmriprep.workflows.bold.t2s.init_bold_t2s_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_t1_trans_wf`
* :py:func:`~fmriprep.workflows.bold.registration.init_bold_reg_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_bold_confounds_wf`
* :py:func:`~fmriprep.workflows.bold.confounds.init_ica_aroma_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_mni_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_preproc_trans_wf`
* :py:func:`~fmriprep.workflows.bold.resampling.init_bold_surf_wf`
* :py:func:`~fmriprep.workflows.fieldmap.pepolar.init_pepolar_unwarp_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_fmap_estimator_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_sdc_unwarp_wf`
* :py:func:`~fmriprep.workflows.fieldmap.init_nonlinear_sdc_wf`
"""
from ..fieldmap.base import init_sdc_wf # Avoid circular dependency (#1066)
ref_file = bold_file
mem_gb = {'filesize': 1, 'resampled': 1, 'largemem': 1}
bold_tlen = 10
multiecho = isinstance(bold_file, list)
if multiecho:
tes = [layout.get_metadata(echo)['EchoTime'] for echo in bold_file]
ref_file = dict(zip(tes, bold_file))[min(tes)]
if os.path.isfile(ref_file):
bold_tlen, mem_gb = _create_mem_gb(ref_file)
wf_name = _get_wf_name(ref_file)
LOGGER.log(25, ('Creating bold processing workflow for "%s" (%.2f GB / %d TRs). '
'Memory resampled/largemem=%.2f/%.2f GB.'),
ref_file, mem_gb['filesize'], bold_tlen, mem_gb['resampled'], mem_gb['largemem'])
sbref_file = None
# For doc building purposes
if layout is None or bold_file == 'bold_preprocesing':
LOGGER.log(25, 'No valid layout: building empty workflow.')
metadata = {
'RepetitionTime': 2.0,
'SliceTiming': [0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9],
'PhaseEncodingDirection': 'j',
}
fmaps = [{
'type': 'phasediff',
'phasediff': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_phasediff.nii.gz',
'magnitude1': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude1.nii.gz',
'magnitude2': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude2.nii.gz',
}]
run_stc = True
multiecho = False
else:
# Find associated sbref, if possible
entities = layout.parse_file_entities(ref_file)
entities['type'] = 'sbref'
files = layout.get(**entities, extensions=['nii', 'nii.gz'])
refbase = os.path.basename(ref_file)
if 'sbref' in ignore:
LOGGER.info("Single-band reference files ignored.")
elif files and multiecho:
LOGGER.warning("Single-band reference found, but not supported in "
"multi-echo workflows at this time. Ignoring.")
elif files:
sbref_file = files[0].filename
sbbase = os.path.basename(sbref_file)
if len(files) > 1:
LOGGER.warning(
"Multiple single-band reference files found for {}; using "
"{}".format(refbase, sbbase))
else:
LOGGER.log(25, "Using single-band reference file {}".format(sbbase))
else:
LOGGER.log(25, "No single-band-reference found for {}".format(refbase))
metadata = layout.get_metadata(ref_file)
# Find fieldmaps. Options: (phase1|phase2|phasediff|epi|fieldmap|syn)
fmaps = []
if 'fieldmaps' not in ignore:
fmaps = layout.get_fieldmap(ref_file, return_list=True)
for fmap in fmaps:
fmap_type = 'phase1' if fmap['type'] == 'phase' else fmap['type']
fmap['metadata'] = layout.get_metadata(fmap[fmap_type])
# Run SyN if forced or in the absence of fieldmap correction
if force_syn or (use_syn and not fmaps):
fmaps.append({'type': 'syn'})
# Short circuits: (True and True and (False or 'TooShort')) == 'TooShort'
run_stc = ("SliceTiming" in metadata and
'slicetiming' not in ignore and
(_get_series_len(ref_file) > 4 or "TooShort"))
# Check if MEEPI for T2* coregistration target
if t2s_coreg and not multiecho:
LOGGER.warning("No multiecho BOLD images found for T2* coregistration. "
"Using standard EPI-T1 coregistration.")
t2s_coreg = False
# By default, force-bbr for t2s_coreg unless user specifies otherwise
if t2s_coreg and use_bbr is None:
use_bbr = True
# Build workflow
workflow = Workflow(name=wf_name)
workflow.__desc__ = """
Functional data preprocessing
: For each of the {num_bold} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""".format(num_bold=num_bold)
workflow.__postdesc__ = """\
All resamplings can be performed with *a single interpolation
step* by composing all the pertinent transformations (i.e. head-motion
transform matrices, susceptibility distortion correction when available,
and co-registrations to anatomical and template spaces).
Gridded (volumetric) resamplings were performed using `antsApplyTransforms` (ANTs),
configured with Lanczos interpolation to minimize the smoothing
effects of other kernels [@lanczos].
Non-gridded (surface) resamplings were performed using `mri_vol2surf`
(FreeSurfer).
"""
inputnode = pe.Node(niu.IdentityInterface(
fields=['bold_file', 'sbref_file', 'subjects_dir', 'subject_id',
't1_preproc', 't1_brain', 't1_mask', 't1_seg', 't1_tpms',
't1_aseg', 't1_aparc',
't1_2_mni_forward_transform', 't1_2_mni_reverse_transform',
't1_2_fsnative_forward_transform', 't1_2_fsnative_reverse_transform']),
name='inputnode')
inputnode.inputs.bold_file = bold_file
if sbref_file is not None:
inputnode.inputs.sbref_file = sbref_file
outputnode = pe.Node(niu.IdentityInterface(
fields=['bold_t1', 'bold_t1_ref', 'bold_mask_t1', 'bold_aseg_t1', 'bold_aparc_t1',
'bold_mni', 'bold_mni_ref' 'bold_mask_mni', 'bold_aseg_mni', 'bold_aparc_mni',
'bold_cifti', 'cifti_variant', 'cifti_variant_key', 'confounds', 'surfaces',
'aroma_noise_ics', 'melodic_mix', 'nonaggr_denoised_file']),
name='outputnode')
# BOLD buffer: an identity used as a pointer to either the original BOLD
# or the STC'ed one for further use.
boldbuffer = pe.Node(niu.IdentityInterface(fields=['bold_file']), name='boldbuffer')
summary = pe.Node(
FunctionalSummary(output_spaces=output_spaces,
slice_timing=run_stc,
registration='FreeSurfer' if freesurfer else 'FSL',
registration_dof=bold2t1w_dof,
pe_direction=metadata.get("PhaseEncodingDirection")),
name='summary', mem_gb=DEFAULT_MEMORY_MIN_GB, run_without_submitting=True)
func_derivatives_wf = init_func_derivatives_wf(output_dir=output_dir,
output_spaces=output_spaces,
template=template,
freesurfer=freesurfer,
use_aroma=use_aroma,
cifti_output=cifti_output)
workflow.connect([
(outputnode, func_derivatives_wf, [
('bold_t1', 'inputnode.bold_t1'),
('bold_t1_ref', 'inputnode.bold_t1_ref'),
('bold_aseg_t1', 'inputnode.bold_aseg_t1'),
('bold_aparc_t1', 'inputnode.bold_aparc_t1'),
('bold_mask_t1', 'inputnode.bold_mask_t1'),
('bold_mni', 'inputnode.bold_mni'),
('bold_mni_ref', 'inputnode.bold_mni_ref'),
('bold_aseg_mni', 'inputnode.bold_aseg_mni'),
('bold_aparc_mni', 'inputnode.bold_aparc_mni'),
('bold_mask_mni', 'inputnode.bold_mask_mni'),
('confounds', 'inputnode.confounds'),
('surfaces', 'inputnode.surfaces'),
('aroma_noise_ics', 'inputnode.aroma_noise_ics'),
('melodic_mix', 'inputnode.melodic_mix'),
('nonaggr_denoised_file', 'inputnode.nonaggr_denoised_file'),
('bold_cifti', 'inputnode.bold_cifti'),
('cifti_variant', 'inputnode.cifti_variant'),
('cifti_variant_key', 'inputnode.cifti_variant_key')
]),
])
# Generate a tentative boldref
bold_reference_wf = init_bold_reference_wf(omp_nthreads=omp_nthreads)
# Top-level BOLD splitter
bold_split = pe.Node(FSLSplit(dimension='t'), name='bold_split',
mem_gb=mem_gb['filesize'] * 3)
# HMC on the BOLD
bold_hmc_wf = init_bold_hmc_wf(name='bold_hmc_wf',
mem_gb=mem_gb['filesize'],
omp_nthreads=omp_nthreads)
# calculate BOLD registration to T1w
bold_reg_wf = init_bold_reg_wf(name='bold_reg_wf',
freesurfer=freesurfer,
use_bbr=use_bbr,
bold2t1w_dof=bold2t1w_dof,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# apply BOLD registration to T1w
bold_t1_trans_wf = init_bold_t1_trans_wf(name='bold_t1_trans_wf',
freesurfer=freesurfer,
use_fieldwarp=(fmaps is not None or use_syn),
multiecho=multiecho,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=False)
# get confounds
bold_confounds_wf = init_bold_confs_wf(
mem_gb=mem_gb['largemem'],
metadata=metadata,
name='bold_confounds_wf')
bold_confounds_wf.get_node('inputnode').inputs.t1_transform_flags = [False]
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_bold_trans_wf = init_bold_preproc_trans_wf(
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_compression=not low_mem,
use_fieldwarp=(fmaps is not None or use_syn),
name='bold_bold_trans_wf'
)
bold_bold_trans_wf.inputs.inputnode.name_source = ref_file
# SLICE-TIME CORRECTION (or bypass) #############################################
if run_stc is True: # bool('TooShort') == True, so check True explicitly
bold_stc_wf = init_bold_stc_wf(name='bold_stc_wf', metadata=metadata)
workflow.connect([
(bold_reference_wf, bold_stc_wf, [
('outputnode.skip_vols', 'inputnode.skip_vols')]),
(bold_stc_wf, boldbuffer, [('outputnode.stc_file', 'bold_file')]),
])
if not multiecho:
workflow.connect([
(bold_reference_wf, bold_stc_wf, [
('outputnode.bold_file', 'inputnode.bold_file')])])
else: # for meepi, iterate through stc_wf for all workflows
meepi_echos = boldbuffer.clone(name='meepi_echos')
meepi_echos.iterables = ('bold_file', bold_file)
workflow.connect([
(meepi_echos, bold_stc_wf, [('bold_file', 'inputnode.bold_file')])])
elif not multiecho: # STC is too short or False
# bypass STC from original BOLD to the splitter through boldbuffer
workflow.connect([
(bold_reference_wf, boldbuffer, [('outputnode.bold_file', 'bold_file')])])
else:
# for meepi, iterate over all meepi echos to boldbuffer
boldbuffer.iterables = ('bold_file', bold_file)
# SDC (SUSCEPTIBILITY DISTORTION CORRECTION) or bypass ##########################
bold_sdc_wf = init_sdc_wf(
fmaps, metadata, omp_nthreads=omp_nthreads,
debug=debug, fmap_demean=fmap_demean, fmap_bspline=fmap_bspline)
bold_sdc_wf.inputs.inputnode.template = template
if not fmaps:
LOGGER.warning('SDC: no fieldmaps found or they were ignored (%s).',
ref_file)
elif fmaps[0]['type'] == 'syn':
LOGGER.warning(
'SDC: no fieldmaps found or they were ignored. '
'Using EXPERIMENTAL "fieldmap-less SyN" correction '
'for dataset %s.', ref_file)
else:
LOGGER.log(25, 'SDC: fieldmap estimation of type "%s" intended for %s found.',
fmaps[0]['type'], ref_file)
# MULTI-ECHO EPI DATA #############################################
if multiecho:
from .util import init_skullstrip_bold_wf
skullstrip_bold_wf = init_skullstrip_bold_wf(name='skullstrip_bold_wf')
inputnode.inputs.bold_file = ref_file # Replace reference w first echo
join_echos = pe.JoinNode(niu.IdentityInterface(fields=['bold_files']),
joinsource=('meepi_echos' if run_stc is True else 'boldbuffer'),
joinfield=['bold_files'],
name='join_echos')
# create optimal combination, adaptive T2* map
bold_t2s_wf = init_bold_t2s_wf(echo_times=tes,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
t2s_coreg=t2s_coreg,
name='bold_t2smap_wf')
workflow.connect([
(skullstrip_bold_wf, join_echos, [
('outputnode.skull_stripped_file', 'bold_files')]),
(join_echos, bold_t2s_wf, [
('bold_files', 'inputnode.bold_file')]),
])
# MAIN WORKFLOW STRUCTURE #######################################################
workflow.connect([
# Generate early reference
(inputnode, bold_reference_wf, [('bold_file', 'inputnode.bold_file'),
('sbref_file', 'inputnode.sbref_file')]),
# BOLD buffer has slice-time corrected if it was run, original otherwise
(boldbuffer, bold_split, [('bold_file', 'in_file')]),
# HMC
(bold_reference_wf, bold_hmc_wf, [
('outputnode.raw_ref_image', 'inputnode.raw_ref_image'),
('outputnode.bold_file', 'inputnode.bold_file')]),
# EPI-T1 registration workflow
(inputnode, bold_reg_wf, [
('t1_brain', 'inputnode.t1_brain'),
('t1_seg', 'inputnode.t1_seg'),
# Undefined if --no-freesurfer, but this is safe
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1_2_fsnative_reverse_transform', 'inputnode.t1_2_fsnative_reverse_transform')]),
(inputnode, bold_t1_trans_wf, [
('bold_file', 'inputnode.name_source'),
('t1_brain', 'inputnode.t1_brain'),
('t1_mask', 'inputnode.t1_mask'),
('t1_aseg', 'inputnode.t1_aseg'),
('t1_aparc', 'inputnode.t1_aparc')]),
# unused if multiecho, but this is safe
(bold_hmc_wf, bold_t1_trans_wf, [('outputnode.xforms', 'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_t1_trans_wf, [
('outputnode.itk_bold_to_t1', 'inputnode.itk_bold_to_t1')]),
(bold_t1_trans_wf, outputnode, [('outputnode.bold_t1', 'bold_t1'),
('outputnode.bold_t1_ref', 'bold_t1_ref'),
('outputnode.bold_aseg_t1', 'bold_aseg_t1'),
('outputnode.bold_aparc_t1', 'bold_aparc_t1')]),
(bold_reg_wf, summary, [('outputnode.fallback', 'fallback')]),
# SDC (or pass-through workflow)
(inputnode, bold_sdc_wf, [
('t1_brain', 'inputnode.t1_brain'),
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform')]),
(bold_reference_wf, bold_sdc_wf, [
('outputnode.ref_image', 'inputnode.bold_ref'),
('outputnode.ref_image_brain', 'inputnode.bold_ref_brain'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
# For t2s_coreg, replace EPI-to-T1w registration inputs
(bold_sdc_wf if not t2s_coreg else bold_t2s_wf, bold_reg_wf, [
('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain')]),
(bold_sdc_wf if not t2s_coreg else bold_t2s_wf, bold_t1_trans_wf, [
('outputnode.bold_ref_brain', 'inputnode.ref_bold_brain'),
('outputnode.bold_mask', 'inputnode.ref_bold_mask')]),
(bold_sdc_wf, bold_t1_trans_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_sdc_wf, bold_bold_trans_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, summary, [('outputnode.method', 'distortion_correction')]),
# Connect bold_confounds_wf
(inputnode, bold_confounds_wf, [('t1_tpms', 'inputnode.t1_tpms'),
('t1_mask', 'inputnode.t1_mask')]),
(bold_hmc_wf, bold_confounds_wf, [
('outputnode.movpar_file', 'inputnode.movpar_file')]),
(bold_reg_wf, bold_confounds_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.t1_bold_xform')]),
(bold_reference_wf, bold_confounds_wf, [
('outputnode.skip_vols', 'inputnode.skip_vols')]),
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
# Connect bold_bold_trans_wf
(bold_split, bold_bold_trans_wf, [
('out_files', 'inputnode.bold_file')]),
(bold_hmc_wf, bold_bold_trans_wf, [
('outputnode.xforms', 'inputnode.hmc_xforms')]),
# Summary
(outputnode, summary, [('confounds', 'confounds_file')]),
])
# for standard EPI data, pass along correct file
if not multiecho:
workflow.connect([
(inputnode, func_derivatives_wf, [
('bold_file', 'inputnode.source_file')]),
(bold_bold_trans_wf, bold_confounds_wf, [
('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_split, bold_t1_trans_wf, [
('out_files', 'inputnode.bold_split')]),
])
else: # for meepi, create and use optimal combination
workflow.connect([
# update name source for optimal combination
(inputnode, func_derivatives_wf, [
(('bold_file', combine_meepi_source), 'inputnode.source_file')]),
(bold_bold_trans_wf, skullstrip_bold_wf, [
('outputnode.bold', 'inputnode.in_file')]),
(bold_t2s_wf, bold_confounds_wf, [
('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_t2s_wf, bold_t1_trans_wf, [
('outputnode.bold', 'inputnode.bold_split')]),
])
if fmaps:
from ..fieldmap.unwarp import init_fmap_unwarp_report_wf
sdc_type = fmaps[0]['type']
# Report on BOLD correction
fmap_unwarp_report_wf = init_fmap_unwarp_report_wf(
suffix='sdc_%s' % sdc_type)
workflow.connect([
(inputnode, fmap_unwarp_report_wf, [
('t1_seg', 'inputnode.in_seg')]),
(bold_reference_wf, fmap_unwarp_report_wf, [
('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, fmap_unwarp_report_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.in_xfm')]),
(bold_sdc_wf, fmap_unwarp_report_wf, [
('outputnode.bold_ref', 'inputnode.in_post')]),
])
if force_syn and sdc_type != 'syn':
syn_unwarp_report_wf = init_fmap_unwarp_report_wf(
suffix='forcedsyn', name='syn_unwarp_report_wf')
workflow.connect([
(inputnode, syn_unwarp_report_wf, [
('t1_seg', 'inputnode.in_seg')]),
(bold_reference_wf, syn_unwarp_report_wf, [
('outputnode.ref_image', 'inputnode.in_pre')]),
(bold_reg_wf, syn_unwarp_report_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.in_xfm')]),
(bold_sdc_wf, syn_unwarp_report_wf, [
('outputnode.syn_bold_ref', 'inputnode.in_post')]),
])
# Map final BOLD mask into T1w space (if required)
if 'T1w' in output_spaces:
from niworkflows.interfaces.fixes import (
FixHeaderApplyTransforms as ApplyTransforms
)
boldmask_to_t1w = pe.Node(
ApplyTransforms(interpolation='MultiLabel', float=True),
name='boldmask_to_t1w', mem_gb=0.1
)
workflow.connect([
(bold_reg_wf, boldmask_to_t1w, [
('outputnode.itk_bold_to_t1', 'transforms')]),
(bold_t1_trans_wf, boldmask_to_t1w, [
('outputnode.bold_mask_t1', 'reference_image')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, boldmask_to_t1w, [
('outputnode.bold_mask', 'input_image')]),
(boldmask_to_t1w, outputnode, [
('output_image', 'bold_mask_t1')]),
])
if 'template' in output_spaces:
# Apply transforms in 1 shot
# Only use uncompressed output if AROMA is to be run
bold_mni_trans_wf = init_bold_mni_trans_wf(
template=template,
freesurfer=freesurfer,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
template_out_grid=template_out_grid,
use_compression=not low_mem,
use_fieldwarp=fmaps is not None,
name='bold_mni_trans_wf'
)
carpetplot_wf = init_carpetplot_wf(
mem_gb=mem_gb['resampled'],
metadata=metadata,
name='carpetplot_wf')
workflow.connect([
(inputnode, bold_mni_trans_wf, [
('bold_file', 'inputnode.name_source'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform'),
('t1_aseg', 'inputnode.bold_aseg'),
('t1_aparc', 'inputnode.bold_aparc')]),
(bold_hmc_wf, bold_mni_trans_wf, [
('outputnode.xforms', 'inputnode.hmc_xforms')]),
(bold_reg_wf, bold_mni_trans_wf, [
('outputnode.itk_bold_to_t1', 'inputnode.itk_bold_to_t1')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, bold_mni_trans_wf, [
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, bold_mni_trans_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_mni_trans_wf, outputnode, [('outputnode.bold_mni', 'bold_mni'),
('outputnode.bold_mni_ref', 'bold_mni_ref'),
('outputnode.bold_mask_mni', 'bold_mask_mni'),
('outputnode.bold_aseg_mni', 'bold_aseg_mni'),
('outputnode.bold_aparc_mni', 'bold_aparc_mni')]),
(inputnode, carpetplot_wf, [
('t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, carpetplot_wf, [
('outputnode.bold', 'inputnode.bold'),
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_reg_wf, carpetplot_wf, [
('outputnode.itk_t1_to_bold', 'inputnode.t1_bold_xform')]),
(bold_confounds_wf, carpetplot_wf, [
('outputnode.confounds_file', 'inputnode.confounds_file')]),
])
if not multiecho:
workflow.connect([
(bold_split, bold_mni_trans_wf, [
('out_files', 'inputnode.bold_split')])
])
else:
split_opt_comb = bold_split.clone(name='split_opt_comb')
workflow.connect([
(bold_t2s_wf, split_opt_comb, [
('outputnode.bold', 'in_file')]),
(split_opt_comb, bold_mni_trans_wf, [
('out_files', 'inputnode.bold_split')
])
])
if use_aroma:
# ICA-AROMA workflow
# Internally resamples to MNI152 Linear (2006)
from .confounds import init_ica_aroma_wf
from niworkflows.interfaces.utils import JoinTSVColumns
ica_aroma_wf = init_ica_aroma_wf(
template=template,
metadata=metadata,
mem_gb=mem_gb['resampled'],
omp_nthreads=omp_nthreads,
use_fieldwarp=fmaps is not None,
ignore_aroma_err=ignore_aroma_err,
aroma_melodic_dim=aroma_melodic_dim,
name='ica_aroma_wf')
join = pe.Node(JoinTSVColumns(), name='aroma_confounds')
workflow.disconnect([
(bold_confounds_wf, outputnode, [
('outputnode.confounds_file', 'confounds'),
]),
])
workflow.connect([
(inputnode, ica_aroma_wf, [
('bold_file', 'inputnode.name_source'),
('t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform')]),
(bold_split, ica_aroma_wf, [
('out_files', 'inputnode.bold_split')]),
(bold_hmc_wf, ica_aroma_wf, [
('outputnode.movpar_file', 'inputnode.movpar_file'),
('outputnode.xforms', 'inputnode.hmc_xforms')]),
(bold_reg_wf, ica_aroma_wf, [
('outputnode.itk_bold_to_t1', 'inputnode.itk_bold_to_t1')]),
(bold_bold_trans_wf if not multiecho else bold_t2s_wf, ica_aroma_wf, [
('outputnode.bold_mask', 'inputnode.bold_mask')]),
(bold_sdc_wf, ica_aroma_wf, [
('outputnode.out_warp', 'inputnode.fieldwarp')]),
(bold_reference_wf, ica_aroma_wf, [
('outputnode.skip_vols', 'inputnode.skip_vols')]),
(bold_confounds_wf, join, [
('outputnode.confounds_file', 'in_file')]),
(ica_aroma_wf, join,
[('outputnode.aroma_confounds', 'join_file')]),
(ica_aroma_wf, outputnode,
[('outputnode.aroma_noise_ics', 'aroma_noise_ics'),
('outputnode.melodic_mix', 'melodic_mix'),
('outputnode.nonaggr_denoised_file', 'nonaggr_denoised_file')]),
(join, outputnode, [('out_file', 'confounds')]),
])
# SURFACES ##################################################################################
surface_spaces = [space for space in output_spaces if space.startswith('fs')]
if freesurfer and surface_spaces:
LOGGER.log(25, 'Creating BOLD surface-sampling workflow.')
bold_surf_wf = init_bold_surf_wf(mem_gb=mem_gb['resampled'],
output_spaces=surface_spaces,
medial_surface_nan=medial_surface_nan,
name='bold_surf_wf')
workflow.connect([
(inputnode, bold_surf_wf, [
('t1_preproc', 'inputnode.t1_preproc'),
('subjects_dir', 'inputnode.subjects_dir'),
('subject_id', 'inputnode.subject_id'),
('t1_2_fsnative_forward_transform', 'inputnode.t1_2_fsnative_forward_transform')]),
(bold_t1_trans_wf, bold_surf_wf, [('outputnode.bold_t1', 'inputnode.source_file')]),
(bold_surf_wf, outputnode, [('outputnode.surfaces', 'surfaces')]),
])
# CIFTI output
if cifti_output and surface_spaces:
bold_surf_wf.__desc__ += """\
*Grayordinates* files [@hcppipelines], which combine surface-sampled
data and volume-sampled data, were also generated.
"""
gen_cifti = pe.MapNode(GenerateCifti(), iterfield=["surface_target", "gifti_files"],
name="gen_cifti")
gen_cifti.inputs.TR = metadata.get("RepetitionTime")
gen_cifti.inputs.surface_target = [s for s in surface_spaces
if s.startswith('fsaverage')]
workflow.connect([
(bold_surf_wf, gen_cifti, [
('outputnode.surfaces', 'gifti_files')]),
(inputnode, gen_cifti, [('subjects_dir', 'subjects_dir')]),
(bold_mni_trans_wf, gen_cifti, [('outputnode.bold_mni', 'bold_file')]),
(gen_cifti, outputnode, [('out_file', 'bold_cifti'),
('variant', 'cifti_variant'),
('variant_key', 'cifti_variant_key')]),
])
# REPORTING ############################################################
ds_report_summary = pe.Node(
DerivativesDataSink(suffix='summary'),
name='ds_report_summary', run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
ds_report_validation = pe.Node(
DerivativesDataSink(base_directory=reportlets_dir,
suffix='validation'),
name='ds_report_validation', run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
workflow.connect([
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bold_reference_wf, ds_report_validation, [
('outputnode.validation_report', 'in_file')]),
])
# Fill-in datasinks of reportlets seen so far
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_report'):
workflow.get_node(node).inputs.base_directory = reportlets_dir
workflow.get_node(node).inputs.source_file = ref_file
return workflow
def init_single_subject_wf(subject_id):
"""
Organize the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from nibabies.workflows.tests import mock_config
from nibabies.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf('01')
Parameters
----------
subject_id : :obj:`str`
Subject label for this single-subject workflow.
Inputs
------
subjects_dir : :obj:`str`
FreeSurfer's ``$SUBJECTS_DIR``.
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.bids import BIDSInfo, BIDSDataGrabber
from niworkflows.interfaces.nilearn import NILEARN_VERSION
from niworkflows.utils.bids import collect_data
from niworkflows.utils.spaces import Reference
from .anatomical import init_infant_anat_wf
from ..utils.misc import fix_multi_source_name
name = "single_subject_%s_wf" % subject_id
subject_data = collect_data(
config.execution.layout,
subject_id,
config.execution.task_id,
config.execution.echo_idx,
bids_filters=config.execution.bids_filters,
)[0]
if "flair" in config.workflow.ignore:
subject_data["flair"] = []
if "t2w" in config.workflow.ignore:
subject_data["t2w"] = []
anat_only = config.workflow.anat_only
anat_derivatives = config.execution.anat_derivatives
anat_modality = config.workflow.anat_modality
spaces = config.workflow.spaces
# Make sure we always go through these two checks
if not anat_only and not subject_data["bold"]:
task_id = config.execution.task_id
raise RuntimeError(
"No BOLD images found for participant {} and task {}. "
"All workflows require BOLD images.".format(
subject_id, task_id if task_id else "<all>"))
if anat_derivatives:
from smriprep.utils.bids import collect_derivatives
std_spaces = spaces.get_spaces(nonstandard=False, dim=(3, ))
anat_derivatives = collect_derivatives(
anat_derivatives.absolute(),
subject_id,
std_spaces,
config.workflow.run_reconall,
)
if anat_derivatives is None:
config.loggers.workflow.warning(f"""\
Attempted to access pre-existing anatomical derivatives at \
<{config.execution.anat_derivatives}>, however not all expectations of fMRIPrep \
were met (for participant <{subject_id}>, spaces <{', '.join(std_spaces)}>, \
reconall <{config.workflow.run_reconall}>).""")
if not anat_derivatives and not subject_data[anat_modality]:
raise Exception(
f"No {anat_modality} images found for participant {subject_id}. "
"All workflows require T1w images.")
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPrep* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(
fmriprep_ver=config.environment.version,
nipype_ver=config.environment.nipype_version,
)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://nibabies.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by fMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(nilearn_ver=NILEARN_VERSION)
fmriprep_dir = str(config.execution.fmriprep_dir)
inputnode = pe.Node(niu.IdentityInterface(fields=["subjects_dir"]),
name="inputnode")
bidssrc = pe.Node(
BIDSDataGrabber(
subject_data=subject_data,
anat_only=anat_only,
anat_derivatives=anat_derivatives,
subject_id=subject_id,
),
name="bidssrc",
)
bids_info = pe.Node(
BIDSInfo(bids_dir=config.execution.bids_dir, bids_validate=False),
name="bids_info",
)
summary = pe.Node(
SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False),
),
name="summary",
run_without_submitting=True,
)
about = pe.Node(
AboutSummary(version=config.environment.version,
command=" ".join(sys.argv)),
name="about",
run_without_submitting=True,
)
ds_report_summary = pe.Node(
DerivativesDataSink(
base_directory=fmriprep_dir,
desc="summary",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_summary",
run_without_submitting=True,
)
ds_report_about = pe.Node(
DerivativesDataSink(
base_directory=fmriprep_dir,
desc="about",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_about",
run_without_submitting=True,
)
# Preprocessing of anatomical (includes registration to UNCInfant)
anat_preproc_wf = init_infant_anat_wf(
ants_affine_init=config.workflow.ants_affine_init or True,
age_months=config.workflow.age_months,
anat_modality=anat_modality,
t1w=subject_data['t1w'],
t2w=subject_data['t2w'],
bids_root=config.execution.bids_dir,
existing_derivatives=anat_derivatives,
freesurfer=config.workflow.run_reconall,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=fmriprep_dir,
segmentation_atlases=config.execution.segmentation_atlases_dir,
skull_strip_mode=config.workflow.skull_strip_t1w,
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
sloppy=config.execution.sloppy,
spaces=spaces,
)
# fmt: off
workflow.connect([
(inputnode, anat_preproc_wf, [
('subjects_dir', 'inputnode.subjects_dir'),
]),
(inputnode, summary, [
('subjects_dir', 'subjects_dir'),
]),
(bidssrc, summary, [
('bold', 'bold'),
]),
(bids_info, summary, [
('subject', 'subject_id'),
]),
(bids_info, anat_preproc_wf, [
(('subject', _prefix), 'inputnode.subject_id'),
]),
(
bidssrc,
anat_preproc_wf,
[
('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
# ('roi', 'inputnode.roi'),
# ('flair', 'inputnode.flair'),
]),
(summary, ds_report_summary, [
('out_report', 'in_file'),
]),
(about, ds_report_about, [
('out_report', 'in_file'),
]),
])
if not anat_derivatives:
workflow.connect([
(bidssrc, bids_info, [
(('t1w', fix_multi_source_name), 'in_file'),
]),
(bidssrc, summary, [
('t1w', 't1w'),
('t2w', 't2w'),
]),
(bidssrc, ds_report_summary, [
(('t1w', fix_multi_source_name), 'source_file'),
]),
(bidssrc, ds_report_about, [
(('t1w', fix_multi_source_name), 'source_file'),
]),
])
else:
workflow.connect([
(bidssrc, bids_info, [
(('bold', fix_multi_source_name), 'in_file'),
]),
(anat_preproc_wf, summary, [
('outputnode.t1w_preproc', 't1w'),
]),
(anat_preproc_wf, ds_report_summary, [
('outputnode.t1w_preproc', 'source_file'),
]),
(anat_preproc_wf, ds_report_about, [
('outputnode.t1w_preproc', 'source_file'),
]),
])
# fmt: on
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split(".")[-1].startswith("ds_"):
workflow.get_node(node).interface.out_path_base = ""
if anat_only:
return workflow
raise NotImplementedError("BOLD processing is not yet implemented.")
# Append the functional section to the existing anatomical exerpt
# That way we do not need to stream down the number of bold datasets
anat_preproc_wf.__postdesc__ = ((anat_preproc_wf.__postdesc__ or "") + f"""
Functional data preprocessing
: For each of the {len(subject_data['bold'])} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""")
for bold_file in subject_data["bold"]:
func_preproc_wf = init_func_preproc_wf(bold_file)
# fmt: off
workflow.connect([
(
anat_preproc_wf,
func_preproc_wf,
[
('outputnode.anat_preproc', 'inputnode.anat_preproc'),
('outputnode.anat_mask', 'inputnode.anat_mask'),
('outputnode.anat_dseg', 'inputnode.anat_dseg'),
('outputnode.anat_aseg', 'inputnode.anat_aseg'),
('outputnode.anat_aparc', 'inputnode.anat_aparc'),
('outputnode.anat_tpms', 'inputnode.anat_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm'),
# Undefined if --fs-no-reconall, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.anat2fsnative_xfm',
'inputnode.t1w2fsnative_xfm'),
('outputnode.fsnative2anat_xfm',
'inputnode.fsnative2t1w_xfm'),
]),
])
# fmt: on
return workflow
def init_ica_aroma_wf(
mem_gb,
metadata,
omp_nthreads,
aroma_melodic_dim=-200,
err_on_aroma_warn=False,
name='ica_aroma_wf',
susan_fwhm=6.0,
):
"""
Build a workflow that runs `ICA-AROMA`_.
This workflow wraps `ICA-AROMA`_ to identify and remove motion-related
independent components from a BOLD time series.
The following steps are performed:
#. Remove non-steady state volumes from the bold series.
#. Smooth data using FSL `susan`, with a kernel width FWHM=6.0mm.
#. Run FSL `melodic` outside of ICA-AROMA to generate the report
#. Run ICA-AROMA
#. Aggregate identified motion components (aggressive) to TSV
#. Return ``classified_motion_ICs`` and ``melodic_mix`` for user to complete
non-aggressive denoising in T1w space
#. Calculate ICA-AROMA-identified noise components
(columns named ``AROMAAggrCompXX``)
Additionally, non-aggressive denoising is performed on the BOLD series
resampled into MNI space.
There is a current discussion on whether other confounds should be extracted
before or after denoising `here
<http://nbviewer.jupyter.org/github/poldracklab/fmriprep-notebooks/blob/922e436429b879271fa13e76767a6e73443e74d9/issue-817_aroma_confounds.ipynb>`__.
.. _ICA-AROMA: https://github.com/maartenmennes/ICA-AROMA
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.bold.confounds import init_ica_aroma_wf
wf = init_ica_aroma_wf(
mem_gb=3,
metadata={'RepetitionTime': 1.0},
omp_nthreads=1)
Parameters
----------
metadata : :obj:`dict`
BIDS metadata for BOLD file
mem_gb : :obj:`float`
Size of BOLD file in GB
omp_nthreads : :obj:`int`
Maximum number of threads an individual process may use
name : :obj:`str`
Name of workflow (default: ``bold_tpl_trans_wf``)
susan_fwhm : :obj:`float`
Kernel width (FWHM in mm) for the smoothing step with
FSL ``susan`` (default: 6.0mm)
err_on_aroma_warn : :obj:`bool`
Do not fail on ICA-AROMA errors
aroma_melodic_dim : :obj:`int`
Set the dimensionality of the MELODIC ICA decomposition.
Negative numbers set a maximum on automatic dimensionality estimation.
Positive numbers set an exact number of components to extract.
(default: -200, i.e., estimate <=200 components)
Inputs
------
itk_bold_to_t1
Affine transform from ``ref_bold_brain`` to T1 space (ITK format)
anat2std_xfm
ANTs-compatible affine-and-warp transform file
name_source
BOLD series NIfTI file
Used to recover original information lost during processing
skip_vols
number of non steady state volumes
bold_split
Individual 3D BOLD volumes, not motion corrected
bold_mask
BOLD series mask in template space
hmc_xforms
List of affine transforms aligning each volume to ``ref_image`` in ITK format
movpar_file
SPM-formatted motion parameters file
Outputs
-------
aroma_confounds
TSV of confounds identified as noise by ICA-AROMA
aroma_noise_ics
CSV of noise components identified by ICA-AROMA
melodic_mix
FSL MELODIC mixing matrix
nonaggr_denoised_file
BOLD series with non-aggressive ICA-AROMA denoising applied
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.segmentation import ICA_AROMARPT
from niworkflows.interfaces.utility import KeySelect
from niworkflows.interfaces.utils import TSV2JSON
workflow = Workflow(name=name)
workflow.__postdesc__ = """\
Automatic removal of motion artifacts using independent component analysis
[ICA-AROMA, @aroma] was performed on the *preprocessed BOLD on MNI space*
time-series after removal of non-steady state volumes and spatial smoothing
with an isotropic, Gaussian kernel of 6mm FWHM (full-width half-maximum).
Corresponding "non-aggresively" denoised runs were produced after such
smoothing.
Additionally, the "aggressive" noise-regressors were collected and placed
in the corresponding confounds file.
"""
inputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_std',
'bold_mask_std',
'movpar_file',
'name_source',
'skip_vols',
'spatial_reference',
]),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(fields=[
'aroma_confounds', 'aroma_noise_ics', 'melodic_mix',
'nonaggr_denoised_file', 'aroma_metadata'
]),
name='outputnode')
# extract out to BOLD base
select_std = pe.Node(KeySelect(fields=['bold_mask_std', 'bold_std']),
name='select_std',
run_without_submitting=True)
select_std.inputs.key = 'MNI152NLin6Asym_res-2'
rm_non_steady_state = pe.Node(niu.Function(function=_remove_volumes,
output_names=['bold_cut']),
name='rm_nonsteady')
calc_median_val = pe.Node(fsl.ImageStats(op_string='-k %s -p 50'),
name='calc_median_val')
calc_bold_mean = pe.Node(fsl.MeanImage(), name='calc_bold_mean')
def _getusans_func(image, thresh):
return [tuple([image, thresh])]
getusans = pe.Node(niu.Function(function=_getusans_func,
output_names=['usans']),
name='getusans',
mem_gb=0.01)
smooth = pe.Node(fsl.SUSAN(fwhm=susan_fwhm), name='smooth')
# melodic node
melodic = pe.Node(fsl.MELODIC(no_bet=True,
tr_sec=float(metadata['RepetitionTime']),
mm_thresh=0.5,
out_stats=True,
dim=aroma_melodic_dim),
name="melodic")
# ica_aroma node
ica_aroma = pe.Node(ICA_AROMARPT(denoise_type='nonaggr',
generate_report=True,
TR=metadata['RepetitionTime'],
args='-np'),
name='ica_aroma')
add_non_steady_state = pe.Node(niu.Function(function=_add_volumes,
output_names=['bold_add']),
name='add_nonsteady')
# extract the confound ICs from the results
ica_aroma_confound_extraction = pe.Node(
ICAConfounds(err_on_aroma_warn=err_on_aroma_warn),
name='ica_aroma_confound_extraction')
ica_aroma_metadata_fmt = pe.Node(TSV2JSON(index_column='IC',
output=None,
enforce_case=True,
additional_metadata={
'Method': {
'Name':
'ICA-AROMA',
'Version':
getenv(
'AROMA_VERSION',
'n/a')
}
}),
name='ica_aroma_metadata_fmt')
ds_report_ica_aroma = pe.Node(DerivativesDataSink(
desc='aroma', datatype="figures", dismiss_entities=("echo", )),
name='ds_report_ica_aroma',
run_without_submitting=True,
mem_gb=DEFAULT_MEMORY_MIN_GB)
def _getbtthresh(medianval):
return 0.75 * medianval
# connect the nodes
workflow.connect([
(inputnode, select_std, [('spatial_reference', 'keys'),
('bold_std', 'bold_std'),
('bold_mask_std', 'bold_mask_std')]),
(inputnode, ica_aroma, [('movpar_file', 'motion_parameters')]),
(inputnode, rm_non_steady_state, [('skip_vols', 'skip_vols')]),
(select_std, rm_non_steady_state, [('bold_std', 'bold_file')]),
(select_std, calc_median_val, [('bold_mask_std', 'mask_file')]),
(rm_non_steady_state, calc_median_val, [('bold_cut', 'in_file')]),
(rm_non_steady_state, calc_bold_mean, [('bold_cut', 'in_file')]),
(calc_bold_mean, getusans, [('out_file', 'image')]),
(calc_median_val, getusans, [('out_stat', 'thresh')]),
# Connect input nodes to complete smoothing
(rm_non_steady_state, smooth, [('bold_cut', 'in_file')]),
(getusans, smooth, [('usans', 'usans')]),
(calc_median_val, smooth, [(('out_stat', _getbtthresh),
'brightness_threshold')]),
# connect smooth to melodic
(smooth, melodic, [('smoothed_file', 'in_files')]),
(select_std, melodic, [('bold_mask_std', 'mask')]),
# connect nodes to ICA-AROMA
(smooth, ica_aroma, [('smoothed_file', 'in_file')]),
(select_std, ica_aroma, [('bold_mask_std', 'report_mask'),
('bold_mask_std', 'mask')]),
(melodic, ica_aroma, [('out_dir', 'melodic_dir')]),
# generate tsvs from ICA-AROMA
(ica_aroma, ica_aroma_confound_extraction, [('out_dir', 'in_directory')
]),
(inputnode, ica_aroma_confound_extraction, [('skip_vols', 'skip_vols')
]),
(ica_aroma_confound_extraction, ica_aroma_metadata_fmt,
[('aroma_metadata', 'in_file')]),
# output for processing and reporting
(ica_aroma_confound_extraction,
outputnode, [('aroma_confounds', 'aroma_confounds'),
('aroma_noise_ics', 'aroma_noise_ics'),
('melodic_mix', 'melodic_mix')]),
(ica_aroma_metadata_fmt, outputnode, [('output', 'aroma_metadata')]),
(ica_aroma, add_non_steady_state, [('nonaggr_denoised_file',
'bold_cut_file')]),
(select_std, add_non_steady_state, [('bold_std', 'bold_file')]),
(inputnode, add_non_steady_state, [('skip_vols', 'skip_vols')]),
(add_non_steady_state, outputnode, [('bold_add',
'nonaggr_denoised_file')]),
(ica_aroma, ds_report_ica_aroma, [('out_report', 'in_file')]),
])
return workflow
def init_single_subject_wf(subject_id, task_id, echo_idx, name, reportlets_dir, output_dir,
bids_dir, ignore, debug, low_mem, anat_only, longitudinal, t2s_coreg,
omp_nthreads, skull_strip_template, skull_strip_fixed_seed,
freesurfer, output_spaces, template, medial_surface_nan,
cifti_output, hires, use_bbr, bold2t1w_dof, fmap_bspline, fmap_demean,
use_syn, force_syn, template_out_grid,
use_aroma, aroma_melodic_dim, ignore_aroma_err):
"""
This workflow organizes the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.base import init_single_subject_wf
wf = init_single_subject_wf(subject_id='test',
task_id='',
echo_idx=None,
name='single_subject_wf',
reportlets_dir='.',
output_dir='.',
bids_dir='.',
ignore=[],
debug=False,
low_mem=False,
anat_only=False,
longitudinal=False,
t2s_coreg=False,
omp_nthreads=1,
skull_strip_template='OASIS',
skull_strip_fixed_seed=False,
freesurfer=True,
template='MNI152NLin2009cAsym',
output_spaces=['T1w', 'fsnative',
'template', 'fsaverage5'],
medial_surface_nan=False,
cifti_output=False,
hires=True,
use_bbr=True,
bold2t1w_dof=9,
fmap_bspline=False,
fmap_demean=True,
use_syn=True,
force_syn=True,
template_out_grid='native',
use_aroma=False,
aroma_melodic_dim=-200,
ignore_aroma_err=False)
Parameters
subject_id : str
List of subject labels
task_id : str or None
Task ID of BOLD series to preprocess, or ``None`` to preprocess all
echo_idx : int or None
Index of echo to preprocess in multiecho BOLD series,
or ``None`` to preprocess all
name : str
Name of workflow
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
debug : bool
Enable debugging outputs
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
anat_only : bool
Disable functional workflows
longitudinal : bool
Treat multiple sessions as longitudinal (may increase runtime)
See sub-workflows for specific differences
t2s_coreg : bool
For multi-echo EPI, use the calculated T2*-map for T2*-driven coregistration
omp_nthreads : int
Maximum number of threads an individual process may use
skull_strip_template : str
Name of ANTs skull-stripping template ('OASIS' or 'NKI')
skull_strip_fixed_seed : bool
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1
reportlets_dir : str
Directory in which to save reportlets
output_dir : str
Directory in which to save derivatives
bids_dir : str
Root directory of BIDS dataset
freesurfer : bool
Enable FreeSurfer surface reconstruction (may increase runtime)
output_spaces : list
List of output spaces functional images are to be resampled to.
Some parts of pipeline will only be instantiated for some output spaces.
Valid spaces:
- T1w
- template
- fsnative
- fsaverage (or other pre-existing FreeSurfer templates)
template : str
Name of template targeted by ``template`` output space
medial_surface_nan : bool
Replace medial wall values with NaNs on functional GIFTI files
cifti_output : bool
Generate bold CIFTI file in output spaces
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
use_bbr : bool or None
Enable/disable boundary-based registration refinement.
If ``None``, test BBR result for distortion before accepting.
bold2t1w_dof : 6, 9 or 12
Degrees-of-freedom for BOLD-T1w registration
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
force_syn : bool
**Temporary**: Always run SyN-based SDC
template_out_grid : str
Keyword ('native', '1mm' or '2mm') or path of custom reference
image for normalization
use_aroma : bool
Perform ICA-AROMA on MNI-resampled functional series
ignore_aroma_err : bool
Do not fail on ICA-AROMA errors
Inputs
subjects_dir
FreeSurfer SUBJECTS_DIR
"""
if name in ('single_subject_wf', 'single_subject_fmripreptest_wf'):
# for documentation purposes
subject_data = {
't1w': ['/completely/made/up/path/sub-01_T1w.nii.gz'],
'bold': ['/completely/made/up/path/sub-01_task-nback_bold.nii.gz']
}
layout = None
else:
subject_data, layout = collect_data(bids_dir, subject_id, task_id, echo_idx)
# Make sure we always go through these two checks
if not anat_only and subject_data['bold'] == []:
raise Exception("No BOLD images found for participant {} and task {}. "
"All workflows require BOLD images.".format(
subject_id, task_id if task_id else '<all>'))
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPprep* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(fmriprep_ver=__version__, nipype_ver=nipype_ver)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://fmriprep.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### References
""".format(nilearn_ver=nilearn_ver)
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data, anat_only=anat_only),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(), name='bids_info', run_without_submitting=True)
summary = pe.Node(SubjectSummary(output_spaces=output_spaces, template=template),
name='summary', run_without_submitting=True)
about = pe.Node(AboutSummary(version=__version__,
command=' '.join(sys.argv)),
name='about', run_without_submitting=True)
ds_report_summary = pe.Node(
DerivativesDataSink(base_directory=reportlets_dir,
suffix='summary'),
name='ds_report_summary', run_without_submitting=True)
ds_report_about = pe.Node(
DerivativesDataSink(base_directory=reportlets_dir,
suffix='about'),
name='ds_report_about', run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(name="anat_preproc_wf",
skull_strip_template=skull_strip_template,
skull_strip_fixed_seed=skull_strip_fixed_seed,
output_spaces=output_spaces,
template=template,
debug=debug,
longitudinal=longitudinal,
omp_nthreads=omp_nthreads,
freesurfer=freesurfer,
hires=hires,
reportlets_dir=reportlets_dir,
output_dir=output_dir,
num_t1w=len(subject_data['t1w']))
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir', 'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'),
('t2w', 't2w'),
('bold', 'bold')]),
(bids_info, summary, [('subject_id', 'subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(summary, anat_preproc_wf, [('subject_id', 'inputnode.subject_id')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name), 'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name), 'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
if anat_only:
return workflow
for bold_file in subject_data['bold']:
func_preproc_wf = init_func_preproc_wf(bold_file=bold_file,
layout=layout,
ignore=ignore,
freesurfer=freesurfer,
use_bbr=use_bbr,
t2s_coreg=t2s_coreg,
bold2t1w_dof=bold2t1w_dof,
reportlets_dir=reportlets_dir,
output_spaces=output_spaces,
template=template,
medial_surface_nan=medial_surface_nan,
cifti_output=cifti_output,
output_dir=output_dir,
omp_nthreads=omp_nthreads,
low_mem=low_mem,
fmap_bspline=fmap_bspline,
fmap_demean=fmap_demean,
use_syn=use_syn,
force_syn=force_syn,
debug=debug,
template_out_grid=template_out_grid,
use_aroma=use_aroma,
aroma_melodic_dim=aroma_melodic_dim,
ignore_aroma_err=ignore_aroma_err,
num_bold=len(subject_data['bold']))
workflow.connect([
(anat_preproc_wf, func_preproc_wf,
[('outputnode.t1_preproc', 'inputnode.t1_preproc'),
('outputnode.t1_brain', 'inputnode.t1_brain'),
('outputnode.t1_mask', 'inputnode.t1_mask'),
('outputnode.t1_seg', 'inputnode.t1_seg'),
('outputnode.t1_aseg', 'inputnode.t1_aseg'),
('outputnode.t1_aparc', 'inputnode.t1_aparc'),
('outputnode.t1_tpms', 'inputnode.t1_tpms'),
('outputnode.t1_2_mni_forward_transform', 'inputnode.t1_2_mni_forward_transform'),
('outputnode.t1_2_mni_reverse_transform', 'inputnode.t1_2_mni_reverse_transform'),
# Undefined if --no-freesurfer, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.t1_2_fsnative_forward_transform',
'inputnode.t1_2_fsnative_forward_transform'),
('outputnode.t1_2_fsnative_reverse_transform',
'inputnode.t1_2_fsnative_reverse_transform')]),
])
return workflow
def init_sdc_wf(fmaps,
bold_meta,
omp_nthreads=1,
debug=False,
fmap_bspline=False,
fmap_demean=True):
"""
This workflow implements the heuristics to choose a
:abbr:`SDC (susceptibility distortion correction)` strategy.
When no field map information is present within the BIDS inputs,
the EXPERIMENTAL "fieldmap-less SyN" can be performed, using
the ``--use-syn`` argument. When ``--force-syn`` is specified,
then the "fieldmap-less SyN" is always executed and reported
despite of other fieldmaps available with higher priority.
In the latter case (some sort of fieldmap(s) is available and
``--force-syn`` is requested), then the :abbr:`SDC (susceptibility
distortion correction)` method applied is that with the
highest priority.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.fieldmap import init_sdc_wf
wf = init_sdc_wf(
fmaps=[{
'suffix': 'phasediff',
'phasediff': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_phasediff.nii.gz',
'magnitude1': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude1.nii.gz',
'magnitude2': 'sub-03/ses-2/fmap/sub-03_ses-2_run-1_magnitude2.nii.gz',
}],
bold_meta={
'RepetitionTime': 2.0,
'SliceTiming': [0.0, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9],
'PhaseEncodingDirection': 'j',
},
)
**Parameters**
fmaps : list of pybids dicts
A list of dictionaries with the available fieldmaps
(and their metadata using the key ``'metadata'`` for the
case of *epi* fieldmaps)
bold_meta : dict
BIDS metadata dictionary corresponding to the BOLD run
omp_nthreads : int
Maximum number of threads an individual process may use
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
debug : bool
Enable debugging outputs
**Inputs**
bold_ref
A BOLD reference calculated at a previous stage
bold_ref_brain
Same as above, but brain-masked
bold_mask
Brain mask for the BOLD run
t1_brain
T1w image, brain-masked, for the fieldmap-less SyN method
std2anat_xfm
List of standard-to-T1w transforms generated during spatial
normalization (only for the fieldmap-less SyN method).
template : str
Name of template from which prior knowledge will be mapped
into the subject's T1w reference
(only for the fieldmap-less SyN method)
templates : str
Name of templates that index the ``std2anat_xfm`` input list
(only for the fieldmap-less SyN method).
**Outputs**
bold_ref
An unwarped BOLD reference
bold_mask
The corresponding new mask after unwarping
bold_ref_brain
Brain-extracted, unwarped BOLD reference
out_warp
The deformation field to unwarp the susceptibility distortions
syn_bold_ref
If ``--force-syn``, an unwarped BOLD reference with this
method (for reporting purposes)
"""
# TODO: To be removed (filter out unsupported fieldmaps):
fmaps = [fmap for fmap in fmaps if fmap['suffix'] in FMAP_PRIORITY]
workflow = Workflow(name='sdc_wf' if fmaps else 'sdc_bypass_wf')
inputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_ref', 'bold_ref_brain', 'bold_mask', 't1_brain', 'std2anat_xfm',
'template', 'templates'
]),
name='inputnode')
outputnode = pe.Node(niu.IdentityInterface(fields=[
'bold_ref', 'bold_mask', 'bold_ref_brain', 'out_warp', 'syn_bold_ref',
'method'
]),
name='outputnode')
# No fieldmaps - forward inputs to outputs
if not fmaps:
outputnode.inputs.method = 'None'
workflow.connect([
(inputnode, outputnode, [('bold_ref', 'bold_ref'),
('bold_mask', 'bold_mask'),
('bold_ref_brain', 'bold_ref_brain')]),
])
return workflow
workflow.__postdesc__ = """\
Based on the estimated susceptibility distortion, an
unwarped BOLD reference was calculated for a more accurate
co-registration with the anatomical reference.
"""
# In case there are multiple fieldmaps prefer EPI
fmaps.sort(key=lambda fmap: FMAP_PRIORITY[fmap['suffix']])
fmap = fmaps[0]
# PEPOLAR path
if fmap['suffix'] == 'epi':
outputnode.inputs.method = 'PEB/PEPOLAR (phase-encoding based / PE-POLARity)'
# Get EPI polarities and their metadata
epi_fmaps = [(fmap_['epi'],
fmap_['metadata']["PhaseEncodingDirection"])
for fmap_ in fmaps if fmap_['suffix'] == 'epi']
sdc_unwarp_wf = init_pepolar_unwarp_wf(bold_meta=bold_meta,
epi_fmaps=epi_fmaps,
omp_nthreads=omp_nthreads,
name='pepolar_unwarp_wf')
workflow.connect([
(inputnode, sdc_unwarp_wf, [('bold_ref', 'inputnode.in_reference'),
('bold_mask', 'inputnode.in_mask'),
('bold_ref_brain',
'inputnode.in_reference_brain')]),
])
# FIELDMAP path
if fmap['suffix'] in ['fieldmap', 'phasediff']:
outputnode.inputs.method = 'FMB (%s-based)' % fmap['suffix']
# Import specific workflows here, so we don't break everything with one
# unused workflow.
if fmap['suffix'] == 'fieldmap':
from .fmap import init_fmap_wf
fmap_estimator_wf = init_fmap_wf(omp_nthreads=omp_nthreads,
fmap_bspline=fmap_bspline)
# set inputs
fmap_estimator_wf.inputs.inputnode.fieldmap = fmap['fieldmap']
fmap_estimator_wf.inputs.inputnode.magnitude = fmap['magnitude']
if fmap['suffix'] == 'phasediff':
from .phdiff import init_phdiff_wf
fmap_estimator_wf = init_phdiff_wf(omp_nthreads=omp_nthreads)
# set inputs
fmap_estimator_wf.inputs.inputnode.phasediff = fmap['phasediff']
fmap_estimator_wf.inputs.inputnode.magnitude = [
fmap_ for key, fmap_ in sorted(fmap.items())
if key.startswith("magnitude")
]
sdc_unwarp_wf = init_sdc_unwarp_wf(omp_nthreads=omp_nthreads,
fmap_demean=fmap_demean,
debug=debug,
name='sdc_unwarp_wf')
sdc_unwarp_wf.inputs.inputnode.metadata = bold_meta
workflow.connect([
(inputnode, sdc_unwarp_wf, [('bold_ref', 'inputnode.in_reference'),
('bold_ref_brain',
'inputnode.in_reference_brain'),
('bold_mask', 'inputnode.in_mask')]),
(fmap_estimator_wf, sdc_unwarp_wf,
[('outputnode.fmap', 'inputnode.fmap'),
('outputnode.fmap_ref', 'inputnode.fmap_ref'),
('outputnode.fmap_mask', 'inputnode.fmap_mask')]),
])
# FIELDMAP-less path
if any(fm['suffix'] == 'syn' for fm in fmaps):
# Select template
sdc_select_std = pe.Node(KeySelect(fields=['std2anat_xfm']),
name='sdc_select_std',
run_without_submitting=True)
syn_sdc_wf = init_syn_sdc_wf(bold_pe=bold_meta.get(
'PhaseEncodingDirection', None),
omp_nthreads=omp_nthreads)
workflow.connect([
(inputnode, sdc_select_std, [('template', 'key'),
('templates', 'keys'),
('std2anat_xfm', 'std2anat_xfm')]),
(sdc_select_std, syn_sdc_wf, [('std2anat_xfm',
'inputnode.std2anat_xfm')]),
(inputnode, syn_sdc_wf, [('t1_brain', 'inputnode.t1_brain'),
('bold_ref', 'inputnode.bold_ref'),
('bold_ref_brain',
'inputnode.bold_ref_brain'),
('template', 'inputnode.template')]),
])
# XXX Eliminate branch when forcing isn't an option
if fmap['suffix'] == 'syn': # No fieldmaps, but --use-syn
outputnode.inputs.method = 'FLB ("fieldmap-less", SyN-based)'
sdc_unwarp_wf = syn_sdc_wf
else: # --force-syn was called when other fieldmap was present
sdc_unwarp_wf.__desc__ = None
workflow.connect([
(syn_sdc_wf, outputnode, [('outputnode.out_reference',
'syn_bold_ref')]),
])
workflow.connect([
(sdc_unwarp_wf, outputnode, [('outputnode.out_warp', 'out_warp'),
('outputnode.out_reference', 'bold_ref'),
('outputnode.out_reference_brain',
'bold_ref_brain'),
('outputnode.out_mask', 'bold_mask')]),
])
return workflow
def init_single_subject_wf(
debug,
freesurfer,
hires,
layout,
longitudinal,
low_mem,
name,
omp_nthreads,
output_dir,
output_spaces,
reportlets_dir,
skull_strip_fixed_seed,
skull_strip_template,
subject_id,
):
"""
Create a single subject workflow.
This workflow organizes the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
.. workflow::
:graph2use: orig
:simple_form: yes
from collections import OrderedDict, namedtuple
from smriprep.workflows.base import init_single_subject_wf
BIDSLayout = namedtuple('BIDSLayout', ['root'])
wf = init_single_subject_wf(
debug=False,
freesurfer=True,
hires=True,
layout=BIDSLayout('.'),
longitudinal=False,
low_mem=False,
name='single_subject_wf',
omp_nthreads=1,
output_dir='.',
output_spaces=OrderedDict([('MNI152NLin2009cAsym', {}),
('fsaverage5', {})]),
reportlets_dir='.',
skull_strip_fixed_seed=False,
skull_strip_template=('OASIS30ANTs', {}),
subject_id='test',
)
**Parameters**
debug : bool
Enable debugging outputs
freesurfer : bool
Enable FreeSurfer surface reconstruction (may increase runtime)
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
layout : BIDSLayout object
BIDS dataset layout
longitudinal : bool
Treat multiple sessions as longitudinal (may increase runtime)
See sub-workflows for specific differences
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
name : str
Name of workflow
omp_nthreads : int
Maximum number of threads an individual process may use
output_dir : str
Directory in which to save derivatives
output_spaces : OrderedDict
List of spatial normalization targets. Some parts of pipeline will
only be instantiated for some output spaces. Valid spaces:
- Any template identifier from TemplateFlow
- Path to a template folder organized following TemplateFlow's
conventions
reportlets_dir : str
Directory in which to save reportlets
skull_strip_fixed_seed : bool
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1
skull_strip_template : tuple
Name of ANTs skull-stripping template (e.g., 'OASIS30ANTs') and
dictionary of template specifications.
subject_id : str
List of subject labels
**Inputs**
subjects_dir
FreeSurfer SUBJECTS_DIR
"""
from ..interfaces.reports import AboutSummary, SubjectSummary
if name in ('single_subject_wf', 'single_subject_smripreptest_wf'):
# for documentation purposes
subject_data = {
't1w': ['/completely/made/up/path/sub-01_T1w.nii.gz'],
}
else:
subject_data = collect_data(layout, subject_id)[0]
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *sMRIPprep* {smriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(smriprep_ver=__version__, nipype_ver=nipype_ver)
workflow.__postdesc__ = """
For more details of the pipeline, see [the section corresponding
to workflows in *sMRIPrep*'s documentation]\
(https://smriprep.readthedocs.io/en/latest/workflows.html \
"sMRIPrep's documentation").
### References
"""
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=True),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(bids_dir=layout.root),
name='bids_info',
run_without_submitting=True)
summary = pe.Node(SubjectSummary(output_spaces=list(output_spaces.keys())),
name='summary',
run_without_submitting=True)
about = pe.Node(AboutSummary(version=__version__,
command=' '.join(sys.argv)),
name='about',
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='summary', keep_dtype=True),
name='ds_report_summary',
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='about', keep_dtype=True),
name='ds_report_about',
run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=layout.root,
debug=debug,
freesurfer=freesurfer,
hires=hires,
longitudinal=longitudinal,
name="anat_preproc_wf",
num_t1w=len(subject_data['t1w']),
omp_nthreads=omp_nthreads,
output_dir=output_dir,
output_spaces=output_spaces,
reportlets_dir=reportlets_dir,
skull_strip_fixed_seed=skull_strip_fixed_seed,
skull_strip_template=skull_strip_template,
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')
]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix),
'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
return workflow
def init_single_subject_wf(
anat_only,
aroma_melodic_dim,
bold2t1w_dof,
cifti_output,
debug,
dummy_scans,
echo_idx,
err_on_aroma_warn,
fmap_bspline,
fmap_demean,
force_syn,
freesurfer,
hires,
ignore,
layout,
longitudinal,
low_mem,
medial_surface_nan,
name,
omp_nthreads,
output_dir,
reportlets_dir,
regressors_all_comps,
regressors_dvars_th,
regressors_fd_th,
skull_strip_fixed_seed,
skull_strip_template,
spaces,
subject_id,
t2s_coreg,
task_id,
use_aroma,
use_bbr,
use_syn,
):
"""
This workflow organizes the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from collections import namedtuple
from niworkflows.utils.spaces import Reference, SpatialReferences
from fmriprep.workflows.base import init_single_subject_wf
BIDSLayout = namedtuple('BIDSLayout', ['root'])
wf = init_single_subject_wf(
anat_only=False,
aroma_melodic_dim=-200,
bold2t1w_dof=9,
cifti_output=False,
debug=False,
dummy_scans=None,
echo_idx=None,
err_on_aroma_warn=False,
fmap_bspline=False,
fmap_demean=True,
force_syn=True,
freesurfer=True,
hires=True,
ignore=[],
layout=BIDSLayout('.'),
longitudinal=False,
low_mem=False,
medial_surface_nan=False,
name='single_subject_wf',
omp_nthreads=1,
output_dir='.',
reportlets_dir='.',
regressors_all_comps=False,
regressors_dvars_th=1.5,
regressors_fd_th=0.5,
skull_strip_fixed_seed=False,
skull_strip_template=Reference('OASIS30ANTs'),
spaces=SpatialReferences(
spaces=['MNI152Lin',
('fsaverage', {'density': '10k'}),
'T1w',
'fsnative'],
checkpoint=True),
subject_id='test',
t2s_coreg=False,
task_id='',
use_aroma=False,
use_bbr=True,
use_syn=True,
)
Parameters
----------
anat_only : bool
Disable functional workflows
aroma_melodic_dim : int
Maximum number of components identified by MELODIC within ICA-AROMA
(default is -200, i.e., no limitation).
bold2t1w_dof : 6, 9 or 12
Degrees-of-freedom for BOLD-T1w registration
cifti_output : bool
Generate bold CIFTI file in output spaces
debug : bool
Enable debugging outputs
dummy_scans : int or None
Number of volumes to consider as non steady state
echo_idx : int or None
Index of echo to preprocess in multiecho BOLD series,
or ``None`` to preprocess all
err_on_aroma_warn : bool
Do not fail on ICA-AROMA errors
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
force_syn : bool
**Temporary**: Always run SyN-based SDC
freesurfer : bool
Enable FreeSurfer surface reconstruction (may increase runtime)
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
layout : BIDSLayout object
BIDS dataset layout
longitudinal : bool
Treat multiple sessions as longitudinal (may increase runtime)
See sub-workflows for specific differences
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
medial_surface_nan : bool
Replace medial wall values with NaNs on functional GIFTI files
name : str
Name of workflow
omp_nthreads : int
Maximum number of threads an individual process may use
output_dir : str
Directory in which to save derivatives
reportlets_dir : str
Directory in which to save reportlets
regressors_all_comps
Return all CompCor component time series instead of the top fraction
regressors_fd_th
Criterion for flagging framewise displacement outliers
regressors_dvars_th
Criterion for flagging DVARS outliers
skull_strip_fixed_seed : bool
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1
skull_strip_template : tuple
Name of target template for brain extraction with ANTs' ``antsBrainExtraction``,
and corresponding dictionary of output-space modifiers.
subject_id : str
List of subject labels
t2s_coreg : bool
For multi-echo EPI, use the calculated T2*-map for T2*-driven coregistration
spaces : :py:class:`~niworkflows.utils.spaces.SpatialReferences`
A container for storing, organizing, and parsing spatial normalizations. Composed of
:py:class:`~niworkflows.utils.spaces.Reference` objects representing spatial references.
Each ``Reference`` contains a space, which is a string of either TemplateFlow template IDs
(e.g., ``MNI152Lin``, ``MNI152NLin6Asym``, ``MNIPediatricAsym``), nonstandard references
(e.g., ``T1w`` or ``anat``, ``sbref``, ``run``, etc.), or a custom template located in
the TemplateFlow root directory. Each ``Reference`` may also contain a spec, which is a
dictionary with template specifications (e.g., a specification of ``{'resolution': 2}``
would lead to resampling on a 2mm resolution of the space).
task_id : str or None
Task ID of BOLD series to preprocess, or ``None`` to preprocess all
use_aroma : bool
Perform ICA-AROMA on MNI-resampled functional series
use_bbr : bool or None
Enable/disable boundary-based registration refinement.
If ``None``, test BBR result for distortion before accepting.
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
Inputs
------
subjects_dir : str
FreeSurfer's ``$SUBJECTS_DIR``.
"""
if name in ('single_subject_wf', 'single_subject_fmripreptest_wf'):
# for documentation purposes
subject_data = {
't1w': ['/completely/made/up/path/sub-01_T1w.nii.gz'],
'bold': ['/completely/made/up/path/sub-01_task-nback_bold.nii.gz']
}
else:
subject_data = collect_data(layout, subject_id, task_id, echo_idx)[0]
# Make sure we always go through these two checks
if not anat_only and subject_data['bold'] == []:
raise Exception("No BOLD images found for participant {} and task {}. "
"All workflows require BOLD images.".format(
subject_id, task_id if task_id else '<all>'))
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPrep* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(fmriprep_ver=__version__, nipype_ver=nipype_ver)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://fmriprep.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by fMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(nilearn_ver=NILEARN_VERSION)
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data, anat_only=anat_only),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(
bids_dir=layout.root, bids_validate=False), name='bids_info')
summary = pe.Node(SubjectSummary(std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False)),
name='summary', run_without_submitting=True)
about = pe.Node(AboutSummary(version=__version__,
command=' '.join(sys.argv)),
name='about', run_without_submitting=True)
ds_report_summary = pe.Node(
DerivativesDataSink(base_directory=reportlets_dir,
desc='summary', keep_dtype=True),
name='ds_report_summary', run_without_submitting=True)
ds_report_about = pe.Node(
DerivativesDataSink(base_directory=reportlets_dir,
desc='about', keep_dtype=True),
name='ds_report_about', run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=layout.root,
debug=debug,
freesurfer=freesurfer,
hires=hires,
longitudinal=longitudinal,
name="anat_preproc_wf",
num_t1w=len(subject_data['t1w']),
omp_nthreads=omp_nthreads,
output_dir=output_dir,
reportlets_dir=reportlets_dir,
spaces=spaces,
skull_strip_fixed_seed=skull_strip_fixed_seed,
skull_strip_template=skull_strip_template,
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir', 'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'),
('t2w', 't2w'),
('bold', 'bold')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix), 'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name), 'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name), 'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_'):
workflow.get_node(node).interface.out_path_base = 'fmriprep'
if anat_only:
return workflow
for bold_file in subject_data['bold']:
func_preproc_wf = init_func_preproc_wf(
aroma_melodic_dim=aroma_melodic_dim,
bold2t1w_dof=bold2t1w_dof,
bold_file=bold_file,
cifti_output=cifti_output,
debug=debug,
dummy_scans=dummy_scans,
err_on_aroma_warn=err_on_aroma_warn,
fmap_bspline=fmap_bspline,
fmap_demean=fmap_demean,
force_syn=force_syn,
freesurfer=freesurfer,
ignore=ignore,
layout=layout,
low_mem=low_mem,
medial_surface_nan=medial_surface_nan,
num_bold=len(subject_data['bold']),
omp_nthreads=omp_nthreads,
output_dir=output_dir,
reportlets_dir=reportlets_dir,
regressors_all_comps=regressors_all_comps,
regressors_fd_th=regressors_fd_th,
regressors_dvars_th=regressors_dvars_th,
spaces=spaces,
t2s_coreg=t2s_coreg,
use_aroma=use_aroma,
use_bbr=use_bbr,
use_syn=use_syn,
)
workflow.connect([
(anat_preproc_wf, func_preproc_wf,
[(('outputnode.t1w_preproc', _pop), 'inputnode.t1w_preproc'),
('outputnode.t1w_brain', 'inputnode.t1w_brain'),
('outputnode.t1w_mask', 'inputnode.t1w_mask'),
('outputnode.t1w_dseg', 'inputnode.t1w_dseg'),
('outputnode.t1w_aseg', 'inputnode.t1w_aseg'),
('outputnode.t1w_aparc', 'inputnode.t1w_aparc'),
('outputnode.t1w_tpms', 'inputnode.t1w_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm'),
('outputnode.joint_template', 'inputnode.joint_template'),
('outputnode.joint_anat2std_xfm', 'inputnode.joint_anat2std_xfm'),
('outputnode.joint_std2anat_xfm', 'inputnode.joint_std2anat_xfm'),
# Undefined if --fs-no-reconall, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.t1w2fsnative_xfm', 'inputnode.t1w2fsnative_xfm'),
('outputnode.fsnative2t1w_xfm', 'inputnode.fsnative2t1w_xfm')]),
])
return workflow
def init_single_subject_wf(subject_id):
"""
Organize the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.tests import mock_config
from fmriprep.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf('01')
Parameters
----------
subject_id : :obj:`str`
Subject label for this single-subject workflow.
Inputs
------
subjects_dir : :obj:`str`
FreeSurfer's ``$SUBJECTS_DIR``.
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.bids import BIDSInfo, BIDSDataGrabber
from niworkflows.interfaces.nilearn import NILEARN_VERSION
from niworkflows.utils.bids import collect_data
from niworkflows.utils.misc import fix_multi_T1w_source_name
from niworkflows.utils.spaces import Reference
from smriprep.workflows.anatomical import init_anat_preproc_wf
name = "single_subject_%s_wf" % subject_id
subject_data = collect_data(config.execution.layout,
subject_id,
config.execution.task_id,
config.execution.echo_idx,
bids_filters=config.execution.bids_filters)[0]
if 'flair' in config.workflow.ignore:
subject_data['flair'] = []
if 't2w' in config.workflow.ignore:
subject_data['t2w'] = []
anat_only = config.workflow.anat_only
# Make sure we always go through these two checks
if not anat_only and not subject_data['bold']:
task_id = config.execution.task_id
raise RuntimeError(
"No BOLD images found for participant {} and task {}. "
"All workflows require BOLD images.".format(
subject_id, task_id if task_id else '<all>'))
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPrep* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(fmriprep_ver=config.environment.version,
nipype_ver=config.environment.nipype_version)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://fmriprep.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by fMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(nilearn_ver=NILEARN_VERSION)
spaces = config.workflow.spaces
reportlets_dir = str(config.execution.work_dir / 'reportlets')
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only,
subject_id=subject_id),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(bids_dir=config.execution.bids_dir,
bids_validate=False),
name='bids_info')
summary = pe.Node(SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False)),
name='summary',
run_without_submitting=True)
about = pe.Node(AboutSummary(version=config.environment.version,
command=' '.join(sys.argv)),
name='about',
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='summary', keep_dtype=True),
name='ds_report_summary',
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, desc='about', keep_dtype=True),
name='ds_report_about',
run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=str(config.execution.bids_dir),
debug=config.execution.debug is True,
freesurfer=config.workflow.run_reconall,
hires=config.workflow.hires,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=str(config.execution.output_dir),
reportlets_dir=reportlets_dir,
skull_strip_fixed_seed=config.workflow.skull_strip_fixed_seed,
skull_strip_mode=config.workflow.skull_strip_t1w,
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
spaces=spaces,
t1w=subject_data['t1w'],
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')
]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w'), ('bold', 'bold')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix),
'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_'):
workflow.get_node(node).interface.out_path_base = 'fmriprep'
if anat_only:
return workflow
# Append the functional section to the existing anatomical exerpt
# That way we do not need to stream down the number of bold datasets
anat_preproc_wf.__postdesc__ = (anat_preproc_wf.__postdesc__ or '') + """
Functional data preprocessing
: For each of the {num_bold} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""".format(num_bold=len(subject_data['bold']))
for bold_file in subject_data['bold']:
func_preproc_wf = init_func_preproc_wf(bold_file)
workflow.connect([
(
anat_preproc_wf,
func_preproc_wf,
[
('outputnode.t1w_preproc', 'inputnode.t1w_preproc'),
('outputnode.t1w_mask', 'inputnode.t1w_mask'),
('outputnode.t1w_dseg', 'inputnode.t1w_dseg'),
('outputnode.t1w_aseg', 'inputnode.t1w_aseg'),
('outputnode.t1w_aparc', 'inputnode.t1w_aparc'),
('outputnode.t1w_tpms', 'inputnode.t1w_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm'),
# Undefined if --fs-no-reconall, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.t1w2fsnative_xfm',
'inputnode.t1w2fsnative_xfm'),
('outputnode.fsnative2t1w_xfm',
'inputnode.fsnative2t1w_xfm')
]),
])
return workflow
def init_3dQwarp_wf(omp_nthreads=1, debug=False, name="pepolar_estimate_wf"):
"""
Create the PEPOLAR field estimation workflow based on AFNI's ``3dQwarp``.
This workflow takes in two EPI files that MUST have opposed
:abbr:`PE (phase-encoding)` direction.
Therefore, EPIs with orthogonal PE directions are not supported.
Workflow Graph
.. workflow ::
:graph2use: orig
:simple_form: yes
from sdcflows.workflows.fit.pepolar import init_3dQwarp_wf
wf = init_3dQwarp_wf()
Parameters
----------
debug : :obj:`bool`
Whether a fast configuration of topup (less accurate) should be applied.
name : :obj:`str`
Name for this workflow
omp_nthreads : :obj:`int`
Parallelize internal tasks across the number of CPUs given by this option.
Inputs
------
in_data : :obj:`list` of :obj:`str`
A list of two EPI files, the first of which will be taken as reference.
Outputs
-------
fmap : :obj:`str`
The path of the estimated fieldmap.
fmap_ref : :obj:`str`
The path of an unwarped conversion of the first element of ``in_data``.
"""
from nipype.interfaces import afni
from niworkflows.interfaces import CopyHeader
from niworkflows.interfaces.fixes import (
FixHeaderRegistration as Registration,
FixHeaderApplyTransforms as ApplyTransforms,
)
from niworkflows.interfaces.freesurfer import StructuralReference
from niworkflows.func.util import init_enhance_and_skullstrip_bold_wf
from ...utils.misc import front as _front, last as _last
from ...interfaces.utils import Flatten, ConvertWarp
workflow = Workflow(name=name)
workflow.__postdesc__ = f"""{_PEPOLAR_DESC} \
with `3dQwarp` (@afni; AFNI {''.join(['%02d' % v for v in afni.Info().version() or []])}).
"""
inputnode = pe.Node(niu.IdentityInterface(fields=["in_data", "metadata"]),
name="inputnode")
outputnode = pe.Node(niu.IdentityInterface(fields=["fmap", "fmap_ref"]),
name="outputnode")
flatten = pe.Node(Flatten(), name="flatten")
sort_pe = pe.Node(
niu.Function(function=_sorted_pe,
output_names=["sorted", "qwarp_args"]),
name="sort_pe",
run_without_submitting=True,
)
merge_pes = pe.MapNode(
StructuralReference(
auto_detect_sensitivity=True,
initial_timepoint=1,
fixed_timepoint=True, # Align to first image
intensity_scaling=True,
# 7-DOF (rigid + intensity)
no_iteration=True,
subsample_threshold=200,
out_file="template.nii.gz",
),
name="merge_pes",
iterfield=["in_files"],
)
pe0_wf = init_enhance_and_skullstrip_bold_wf(omp_nthreads=omp_nthreads,
name="pe0_wf")
pe1_wf = init_enhance_and_skullstrip_bold_wf(omp_nthreads=omp_nthreads,
name="pe1_wf")
align_pes = pe.Node(
Registration(
from_file=_pkg_fname("sdcflows", "data/translation_rigid.json"),
output_warped_image=True,
),
name="align_pes",
n_procs=omp_nthreads,
)
qwarp = pe.Node(
afni.QwarpPlusMinus(
blur=[-1, -1],
environ={"OMP_NUM_THREADS": f"{min(omp_nthreads, 4)}"},
minpatch=9,
nopadWARP=True,
noweight=True,
pblur=[0.05, 0.05],
),
name="qwarp",
n_procs=min(omp_nthreads, 4),
)
to_ants = pe.Node(ConvertWarp(), name="to_ants", mem_gb=0.01)
cphdr_warp = pe.Node(CopyHeader(), name="cphdr_warp", mem_gb=0.01)
unwarp_reference = pe.Node(
ApplyTransforms(
dimension=3,
float=True,
interpolation="LanczosWindowedSinc",
),
name="unwarp_reference",
)
# fmt: off
workflow.connect([
(inputnode, flatten, [("in_data", "in_data"),
("metadata", "in_meta")]),
(flatten, sort_pe, [("out_list", "inlist")]),
(sort_pe, qwarp, [("qwarp_args", "args")]),
(sort_pe, merge_pes, [("sorted", "in_files")]),
(merge_pes, pe0_wf, [(("out_file", _front), "inputnode.in_file")]),
(merge_pes, pe1_wf, [(("out_file", _last), "inputnode.in_file")]),
(pe0_wf, align_pes, [("outputnode.skull_stripped_file", "fixed_image")
]),
(pe1_wf, align_pes, [("outputnode.skull_stripped_file", "moving_image")
]),
(pe0_wf, qwarp, [("outputnode.skull_stripped_file", "in_file")]),
(align_pes, qwarp, [("warped_image", "base_file")]),
(inputnode, cphdr_warp, [(("in_data", _front), "hdr_file")]),
(qwarp, cphdr_warp, [("source_warp", "in_file")]),
(cphdr_warp, to_ants, [("out_file", "in_file")]),
(to_ants, unwarp_reference, [("out_file", "transforms")]),
(inputnode, unwarp_reference, [("in_reference", "reference_image"),
("in_reference", "input_image")]),
(unwarp_reference, outputnode, [("output_image", "fmap_ref")]),
(to_ants, outputnode, [("out_file", "fmap")]),
])
# fmt: on
return workflow
def init_single_subject_wf(subject_id):
"""
Organize the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from fprodents.workflows.tests import mock_config
from fprodents.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf('01')
Parameters
----------
subject_id : :obj:`str`
Subject label for this single-subject workflow.
Inputs
------
subjects_dir : :obj:`str`
FreeSurfer's ``$SUBJECTS_DIR``.
"""
from niworkflows.engine.workflows import LiterateWorkflow as Workflow
from niworkflows.interfaces.bids import BIDSInfo
from niworkflows.interfaces.nilearn import NILEARN_VERSION
from niworkflows.utils.bids import collect_data
from niworkflows.utils.connections import listify
from niworkflows.utils.spaces import Reference
from niworkflows.workflows.epi.refmap import init_epi_reference_wf
from ..patch.interfaces import BIDSDataGrabber
from ..patch.utils import extract_entities, fix_multi_source_name
from ..patch.workflows.anatomical import init_anat_preproc_wf
subject_data = collect_data(
config.execution.layout,
subject_id,
config.execution.task_id,
config.execution.echo_idx,
bids_filters=config.execution.bids_filters,
)[0]
anat_only = config.workflow.anat_only
# Make sure we always go through these two checks
if not anat_only and not subject_data["bold"]:
task_id = config.execution.task_id
raise RuntimeError(
f"No BOLD images found for participant <{subject_id}> and "
f"task <{task_id or 'all'}>. All workflows require BOLD images.")
workflow = Workflow(name=f"single_subject_{subject_id}_wf")
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPrep-rodents* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(
fmriprep_ver=config.environment.version,
nipype_ver=config.environment.nipype_version,
)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://fmriprep-rodents.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by fMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
""".format(nilearn_ver=NILEARN_VERSION)
spaces = config.workflow.spaces
output_dir = str(config.execution.output_dir)
inputnode = pe.Node(niu.IdentityInterface(fields=["subjects_dir"]),
name="inputnode")
bidssrc = pe.Node(
BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only,
subject_id=subject_id),
name="bidssrc",
)
bids_info = pe.Node(
BIDSInfo(bids_dir=config.execution.bids_dir, bids_validate=False),
name="bids_info",
)
summary = pe.Node(
SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False),
),
name="summary",
run_without_submitting=True,
)
about = pe.Node(
AboutSummary(version=config.environment.version,
command=" ".join(sys.argv)),
name="about",
run_without_submitting=True,
)
ds_report_summary = pe.Node(
DerivativesDataSink(
base_directory=output_dir,
desc="summary",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_summary",
run_without_submitting=True,
)
ds_report_about = pe.Node(
DerivativesDataSink(
base_directory=output_dir,
desc="about",
datatype="figures",
dismiss_entities=("echo", ),
),
name="ds_report_about",
run_without_submitting=True,
)
anat_derivatives = config.execution.anat_derivatives
if anat_derivatives:
from smriprep.utils.bids import collect_derivatives
std_spaces = spaces.get_spaces(nonstandard=False, dim=(3, ))
anat_derivatives = collect_derivatives(
anat_derivatives.absolute(),
subject_id,
std_spaces,
False,
)
if anat_derivatives is None:
config.loggers.workflow.warning(f"""\
Attempted to access pre-existing anatomical derivatives at \
<{config.execution.anat_derivatives}>, however not all expectations of fMRIPrep \
were met (for participant <{subject_id}>, spaces <{', '.join(std_spaces)}>.""")
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=str(config.execution.bids_dir),
debug=config.execution.debug is True,
existing_derivatives=anat_derivatives,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=output_dir,
skull_strip_fixed_seed=config.workflow.skull_strip_fixed_seed,
skull_strip_mode=config.workflow.skull_strip_t1w,
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
spaces=spaces,
t2w=subject_data["t2w"],
)
# fmt:off
workflow.connect([
(bidssrc, bids_info, [(('t2w', fix_multi_source_name), 'in_file')]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w'), ('bold', 'bold')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bidssrc, anat_preproc_wf, [('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi')]),
(bidssrc, ds_report_summary, [(('t2w', fix_multi_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t2w', fix_multi_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
# fmt:on
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split(".")[-1].startswith("ds_"):
workflow.get_node(node).interface.out_path_base = "fmriprep"
if anat_only:
return workflow
# Append the functional section to the existing anatomical exerpt
# That way we do not need to stream down the number of bold datasets
anat_preproc_wf.__postdesc__ = ((anat_preproc_wf.__postdesc__ or "") + """
Functional data preprocessing
: For each of the {num_bold} BOLD runs found per subject (across all
tasks and sessions), the following preprocessing was performed.
""".format(num_bold=len(subject_data["bold"])))
for bold_file in subject_data["bold"]:
echoes = extract_entities(bold_file).get("echo", [])
echo_idxs = listify(echoes)
multiecho = len(echo_idxs) > 2
# The default N4 shrink factor (4) appears to artificially blur values across
# anisotropic voxels. Shrink factors are intended to speed up calculation
# but in most cases, the extra calculation time appears to be minimal.
# Similarly, the use of an asymmetric bspline grid improves performance
# in anisotropic voxels. The number of N4 iterations are also reduced.
bold_ref_wf = init_epi_reference_wf(
auto_bold_nss=True,
omp_nthreads=config.nipype.omp_nthreads,
n4_iter=4,
adaptive_bspline_grid=True,
shrink_factor=1,
)
bold_ref_wf.inputs.inputnode.in_files = (bold_file if not multiecho
else bold_file[0])
func_preproc_wf = init_func_preproc_wf(bold_file)
# fmt:off
workflow.connect([
(anat_preproc_wf, func_preproc_wf,
[('outputnode.t2w_preproc', 'inputnode.anat_preproc'),
('outputnode.t2w_mask', 'inputnode.anat_mask'),
('outputnode.t2w_dseg', 'inputnode.anat_dseg'),
('outputnode.t2w_tpms', 'inputnode.anat_tpms'),
('outputnode.template', 'inputnode.template'),
('outputnode.anat2std_xfm', 'inputnode.anat2std_xfm'),
('outputnode.std2anat_xfm', 'inputnode.std2anat_xfm')]),
(bold_ref_wf, func_preproc_wf,
[('outputnode.epi_ref_file', 'inputnode.ref_file'),
('outputnode.xfm_files', 'inputnode.bold_ref_xfm'),
('outputnode.validation_report', 'inputnode.validation_report'),
(('outputnode.n_dummy', _pop), 'inputnode.n_dummy_scans')]),
])
# fmt:on
return workflow
def init_single_subject_wf(
layout, subject_id, task_id, echo_idx, name, reportlets_dir,
output_dir, ignore, debug, low_mem, anat_only, longitudinal, t2s_coreg,
omp_nthreads, skull_strip_template, skull_strip_fixed_seed, freesurfer,
output_spaces, template, medial_surface_nan, cifti_output, hires,
use_bbr, bold2t1w_dof, fmap_bspline, fmap_demean, use_syn, force_syn,
template_out_grid, use_aroma, aroma_melodic_dim, err_on_aroma_warn):
"""
This workflow organizes the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and functional preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Functional preprocessing is performed using a separate workflow for each
individual BOLD series.
.. workflow::
:graph2use: orig
:simple_form: yes
from fmriprep.workflows.base import init_single_subject_wf
from collections import namedtuple
BIDSLayout = namedtuple('BIDSLayout', ['root'], defaults='.')
wf = init_single_subject_wf(layout=BIDSLayout(),
subject_id='test',
task_id='',
echo_idx=None,
name='single_subject_wf',
reportlets_dir='.',
output_dir='.',
ignore=[],
debug=False,
low_mem=False,
anat_only=False,
longitudinal=False,
t2s_coreg=False,
omp_nthreads=1,
skull_strip_template='OASIS30ANTs',
skull_strip_fixed_seed=False,
freesurfer=True,
template='MNI152NLin2009cAsym',
output_spaces=['T1w', 'fsnative',
'template', 'fsaverage5'],
medial_surface_nan=False,
cifti_output=False,
hires=True,
use_bbr=True,
bold2t1w_dof=9,
fmap_bspline=False,
fmap_demean=True,
use_syn=True,
force_syn=True,
template_out_grid='native',
use_aroma=False,
aroma_melodic_dim=-200,
err_on_aroma_warn=False)
Parameters
layout : BIDSLayout object
BIDS dataset layout
subject_id : str
List of subject labels
task_id : str or None
Task ID of BOLD series to preprocess, or ``None`` to preprocess all
echo_idx : int or None
Index of echo to preprocess in multiecho BOLD series,
or ``None`` to preprocess all
name : str
Name of workflow
ignore : list
Preprocessing steps to skip (may include "slicetiming", "fieldmaps")
debug : bool
Enable debugging outputs
low_mem : bool
Write uncompressed .nii files in some cases to reduce memory usage
anat_only : bool
Disable functional workflows
longitudinal : bool
Treat multiple sessions as longitudinal (may increase runtime)
See sub-workflows for specific differences
t2s_coreg : bool
For multi-echo EPI, use the calculated T2*-map for T2*-driven coregistration
omp_nthreads : int
Maximum number of threads an individual process may use
skull_strip_template : str
Name of ANTs skull-stripping template ('OASIS30ANTs' or 'NKI')
skull_strip_fixed_seed : bool
Do not use a random seed for skull-stripping - will ensure
run-to-run replicability when used with --omp-nthreads 1
reportlets_dir : str
Directory in which to save reportlets
output_dir : str
Directory in which to save derivatives
freesurfer : bool
Enable FreeSurfer surface reconstruction (may increase runtime)
output_spaces : list
List of output spaces functional images are to be resampled to.
Some parts of pipeline will only be instantiated for some output spaces.
Valid spaces:
- T1w
- template
- fsnative
- fsaverage (or other pre-existing FreeSurfer templates)
template : str
Name of template targeted by ``template`` output space
medial_surface_nan : bool
Replace medial wall values with NaNs on functional GIFTI files
cifti_output : bool
Generate bold CIFTI file in output spaces
hires : bool
Enable sub-millimeter preprocessing in FreeSurfer
use_bbr : bool or None
Enable/disable boundary-based registration refinement.
If ``None``, test BBR result for distortion before accepting.
bold2t1w_dof : 6, 9 or 12
Degrees-of-freedom for BOLD-T1w registration
fmap_bspline : bool
**Experimental**: Fit B-Spline field using least-squares
fmap_demean : bool
Demean voxel-shift map during unwarp
use_syn : bool
**Experimental**: Enable ANTs SyN-based susceptibility distortion correction (SDC).
If fieldmaps are present and enabled, this is not run, by default.
force_syn : bool
**Temporary**: Always run SyN-based SDC
template_out_grid : str
Keyword ('native', '1mm' or '2mm') or path of custom reference
image for normalization
use_aroma : bool
Perform ICA-AROMA on MNI-resampled functional series
err_on_aroma_warn : bool
Do not fail on ICA-AROMA errors
Inputs
subjects_dir
FreeSurfer SUBJECTS_DIR
"""
if name in ('single_subject_wf', 'single_subject_fmripreptest_wf'):
# for documentation purposes
subject_data = {
't1w': ['/completely/made/up/path/sub-01_T1w.nii.gz'],
'bold': ['/completely/made/up/path/sub-01_task-nback_bold.nii.gz']
}
else:
subject_data = collect_data(layout, subject_id, task_id, echo_idx)[0]
# Make sure we always go through these two checks
if not anat_only and subject_data['bold'] == []:
raise Exception("No BOLD images found for participant {} and task {}. "
"All workflows require BOLD images.".format(
subject_id, task_id if task_id else '<all>'))
if not subject_data['t1w']:
raise Exception("No T1w images found for participant {}. "
"All workflows require T1w images.".format(subject_id))
workflow = Workflow(name=name)
workflow.__desc__ = """
Results included in this manuscript come from preprocessing
performed using *fMRIPrep* {fmriprep_ver}
(@fmriprep1; @fmriprep2; RRID:SCR_016216),
which is based on *Nipype* {nipype_ver}
(@nipype1; @nipype2; RRID:SCR_002502).
""".format(fmriprep_ver=__version__, nipype_ver=nipype_ver)
workflow.__postdesc__ = """
Many internal operations of *fMRIPrep* use
*Nilearn* {nilearn_ver} [@nilearn, RRID:SCR_001362],
mostly within the functional processing workflow.
For more details of the pipeline, see [the section corresponding
to workflows in *fMRIPrep*'s documentation]\
(https://fmriprep.readthedocs.io/en/latest/workflows.html \
"FMRIPrep's documentation").
### References
""".format(nilearn_ver=nilearn_ver)
inputnode = pe.Node(niu.IdentityInterface(fields=['subjects_dir']),
name='inputnode')
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only),
name='bidssrc')
bids_info = pe.Node(BIDSInfo(bids_dir=layout.root, bids_validate=False),
name='bids_info')
summary = pe.Node(SubjectSummary(output_spaces=output_spaces,
template=template),
name='summary',
run_without_submitting=True)
about = pe.Node(AboutSummary(version=__version__,
command=' '.join(sys.argv)),
name='about',
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, suffix='summary'),
name='ds_report_summary',
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=reportlets_dir, suffix='about'),
name='ds_report_about',
run_without_submitting=True)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=layout.root,
debug=debug,
freesurfer=freesurfer,
fs_spaces=output_spaces,
hires=hires,
longitudinal=longitudinal,
name="anat_preproc_wf",
num_t1w=len(subject_data['t1w']),
omp_nthreads=omp_nthreads,
output_dir=output_dir,
reportlets_dir=reportlets_dir,
skull_strip_fixed_seed=skull_strip_fixed_seed,
skull_strip_template=skull_strip_template,
template=template,
)
workflow.connect([
(inputnode, anat_preproc_wf, [('subjects_dir',
'inputnode.subjects_dir')]),
(bidssrc, bids_info, [(('t1w', fix_multi_T1w_source_name), 'in_file')
]),
(inputnode, summary, [('subjects_dir', 'subjects_dir')]),
(bidssrc, summary, [('t1w', 't1w'), ('t2w', 't2w'), ('bold', 'bold')]),
(bids_info, summary, [('subject', 'subject_id')]),
(bids_info, anat_preproc_wf, [(('subject', _prefix),
'inputnode.subject_id')]),
(bidssrc, anat_preproc_wf, [('t1w', 'inputnode.t1w'),
('t2w', 'inputnode.t2w'),
('roi', 'inputnode.roi'),
('flair', 'inputnode.flair')]),
(bidssrc, ds_report_summary, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(summary, ds_report_summary, [('out_report', 'in_file')]),
(bidssrc, ds_report_about, [(('t1w', fix_multi_T1w_source_name),
'source_file')]),
(about, ds_report_about, [('out_report', 'in_file')]),
])
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split('.')[-1].startswith('ds_'):
workflow.get_node(node).interface.out_path_base = 'fmriprep'
if anat_only:
return workflow
for bold_file in subject_data['bold']:
func_preproc_wf = init_func_preproc_wf(
bold_file=bold_file,
layout=layout,
ignore=ignore,
freesurfer=freesurfer,
use_bbr=use_bbr,
t2s_coreg=t2s_coreg,
bold2t1w_dof=bold2t1w_dof,
reportlets_dir=reportlets_dir,
output_spaces=output_spaces,
template=template,
medial_surface_nan=medial_surface_nan,
cifti_output=cifti_output,
output_dir=output_dir,
omp_nthreads=omp_nthreads,
low_mem=low_mem,
fmap_bspline=fmap_bspline,
fmap_demean=fmap_demean,
use_syn=use_syn,
force_syn=force_syn,
debug=debug,
template_out_grid=template_out_grid,
use_aroma=use_aroma,
aroma_melodic_dim=aroma_melodic_dim,
err_on_aroma_warn=err_on_aroma_warn,
num_bold=len(subject_data['bold']))
workflow.connect([
(
anat_preproc_wf,
func_preproc_wf,
[
(('outputnode.t1_preproc', _pop), 'inputnode.t1_preproc'),
('outputnode.t1_brain', 'inputnode.t1_brain'),
('outputnode.t1_mask', 'inputnode.t1_mask'),
('outputnode.t1_seg', 'inputnode.t1_seg'),
('outputnode.t1_aseg', 'inputnode.t1_aseg'),
('outputnode.t1_aparc', 'inputnode.t1_aparc'),
('outputnode.t1_tpms', 'inputnode.t1_tpms'),
('outputnode.t1_2_mni_forward_transform',
'inputnode.t1_2_mni_forward_transform'),
('outputnode.t1_2_mni_reverse_transform',
'inputnode.t1_2_mni_reverse_transform'),
# Undefined if --no-freesurfer, but this is safe
('outputnode.subjects_dir', 'inputnode.subjects_dir'),
('outputnode.subject_id', 'inputnode.subject_id'),
('outputnode.t1_2_fsnative_forward_transform',
'inputnode.t1_2_fsnative_forward_transform'),
('outputnode.t1_2_fsnative_reverse_transform',
'inputnode.t1_2_fsnative_reverse_transform')
]),
])
return workflow
def init_single_subject_wf(subject_id):
"""
Set-up the preprocessing pipeline for a single subject.
It collects and reports information about the subject, and prepares
sub-workflows to perform anatomical and diffusion MRI preprocessing.
Anatomical preprocessing is performed in a single workflow, regardless of
the number of sessions.
Diffusion MRI preprocessing is performed using a separate workflow for
a full :abbr:`DWI (diffusion weighted imaging)` *entity*.
A DWI *entity* may comprehend one or several runs (for instance, two
opposed :abbr:`PE (phase-encoding)` directions.
Workflow Graph
.. workflow::
:graph2use: orig
:simple_form: yes
from dmriprep.config.testing import mock_config
from dmriprep.workflows.base import init_single_subject_wf
with mock_config():
wf = init_single_subject_wf("THP0005")
Parameters
----------
subject_id : str
List of subject labels
Inputs
------
subjects_dir : os.pathlike
FreeSurfer's ``$SUBJECTS_DIR``
"""
name = f"single_subject_{subject_id}_wf"
subject_data = collect_data(config.execution.layout, subject_id)[0]
if "flair" in config.workflow.ignore:
subject_data["flair"] = []
if "t2w" in config.workflow.ignore:
subject_data["t2w"] = []
anat_only = config.workflow.anat_only
# Make sure we always go through these two checks
if not anat_only and not subject_data["dwi"]:
raise Exception(f"No DWI data found for participant {subject_id}. "
"All workflows require DWI images.")
if not subject_data["t1w"]:
raise Exception(f"No T1w images found for participant {subject_id}. "
"All workflows require T1w images.")
workflow = Workflow(name=name)
workflow.__desc__ = f"""
Results included in this manuscript come from preprocessing
performed using *dMRIPrep* {config.environment.version}
(@dmriprep; RRID:SCR_017412),
which is based on *Nipype* {config.environment.nipype_version}
(@nipype1; @nipype2; RRID:SCR_002502).
"""
workflow.__postdesc__ = """
For more details of the pipeline, see [the section corresponding
to workflows in *dMRIPrep*'s documentation]\
(https://nipreps.github.io/dmriprep/master/workflows.html \
"dMRIPrep's documentation").
### Copyright Waiver
The above boilerplate text was automatically generated by dMRIPrep
with the express intention that users should copy and paste this
text into their manuscripts *unchanged*.
It is released under the [CC0]\
(https://creativecommons.org/publicdomain/zero/1.0/) license.
### References
"""
spaces = config.workflow.spaces
output_dir = config.execution.output_dir
fsinputnode = pe.Node(niu.IdentityInterface(fields=["subjects_dir"]),
name="fsinputnode")
bidssrc = pe.Node(BIDSDataGrabber(subject_data=subject_data,
anat_only=anat_only),
name="bidssrc")
bids_info = pe.Node(BIDSInfo(bids_dir=config.execution.bids_dir,
bids_validate=False),
name="bids_info")
summary = pe.Node(SubjectSummary(
std_spaces=spaces.get_spaces(nonstandard=False),
nstd_spaces=spaces.get_spaces(standard=False)),
name="summary",
run_without_submitting=True)
about = pe.Node(AboutSummary(version=config.environment.version,
command=" ".join(sys.argv)),
name="about",
run_without_submitting=True)
ds_report_summary = pe.Node(DerivativesDataSink(
base_directory=str(output_dir), desc="summary", datatype="figures"),
name="ds_report_summary",
run_without_submitting=True)
ds_report_about = pe.Node(DerivativesDataSink(
base_directory=str(output_dir), desc="about", datatype="figures"),
name="ds_report_about",
run_without_submitting=True)
anat_derivatives = config.execution.anat_derivatives
if anat_derivatives:
from smriprep.utils.bids import collect_derivatives
std_spaces = spaces.get_spaces(nonstandard=False, dim=(3, ))
anat_derivatives = collect_derivatives(
anat_derivatives.absolute(),
subject_id,
std_spaces,
config.workflow.run_reconall,
)
# Preprocessing of T1w (includes registration to MNI)
anat_preproc_wf = init_anat_preproc_wf(
bids_root=str(config.execution.bids_dir),
debug=config.execution.debug is True,
existing_derivatives=anat_derivatives,
freesurfer=config.workflow.run_reconall,
hires=config.workflow.hires,
longitudinal=config.workflow.longitudinal,
omp_nthreads=config.nipype.omp_nthreads,
output_dir=str(output_dir),
skull_strip_fixed_seed=config.workflow.skull_strip_fixed_seed,
skull_strip_mode="force",
skull_strip_template=Reference.from_string(
config.workflow.skull_strip_template)[0],
spaces=spaces,
t1w=subject_data["t1w"],
)
workflow.connect([
(fsinputnode, anat_preproc_wf, [("subjects_dir",
"inputnode.subjects_dir")]),
(bidssrc, bids_info, [(("t1w", fix_multi_T1w_source_name), "in_file")
]),
(fsinputnode, summary, [("subjects_dir", "subjects_dir")]),
(bidssrc, summary, [("t1w", "t1w"), ("t2w", "t2w"), ("dwi", "dwi")]),
(bids_info, summary, [("subject", "subject_id")]),
(bids_info, anat_preproc_wf, [(("subject", _prefix),
"inputnode.subject_id")]),
(bidssrc, anat_preproc_wf, [("t1w", "inputnode.t1w"),
("t2w", "inputnode.t2w"),
("roi", "inputnode.roi"),
("flair", "inputnode.flair")]),
(bidssrc, ds_report_summary, [(("t1w", fix_multi_T1w_source_name),
"source_file")]),
(summary, ds_report_summary, [("out_report", "in_file")]),
(bidssrc, ds_report_about, [(("t1w", fix_multi_T1w_source_name),
"source_file")]),
(about, ds_report_about, [("out_report", "in_file")]),
])
# Overwrite ``out_path_base`` of smriprep's DataSinks
for node in workflow.list_node_names():
if node.split(".")[-1].startswith("ds_"):
workflow.get_node(node).interface.out_path_base = "dmriprep"
if anat_only:
return workflow
# Append the dMRI section to the existing anatomical excerpt
# That way we do not need to stream down the number of bold datasets
anat_preproc_wf.__postdesc__ = (anat_preproc_wf.__postdesc__ or "") + f"""
Diffusion data preprocessing
: For each of the {len(subject_data["dwi"])} DWI scans found per subject
(across all sessions), the gradient table was vetted and converted into the *RASb*
format (i.e., given in RAS+ scanner coordinates, normalized b-vectors and scaled b-values),
and a *b=0* average for reference to the subsequent steps of preprocessing was calculated.
"""
layout = config.execution.layout
dwi_data = tuple([(dwi, layout.get_metadata(dwi), layout.get_bvec(dwi),
layout.get_bval(dwi)) for dwi in subject_data["dwi"]])
inputnode = pe.Node(niu.IdentityInterface(fields=["dwi_data"]),
name="inputnode")
inputnode.iterables = [("dwi_data", dwi_data)]
referencenode = pe.JoinNode(niu.IdentityInterface(fields=[
"dwi_file", "metadata", "dwi_reference", "dwi_mask", "gradients_rasb"
]),
name="referencenode",
joinsource="inputnode",
run_without_submitting=True)
split_info = pe.Node(niu.Function(
function=_unpack,
output_names=["dwi_file", "metadata", "bvec", "bval"]),
name="split_info",
run_without_submitting=True)
early_b0ref_wf = init_early_b0ref_wf()
workflow.connect([
(inputnode, split_info, [("dwi_data", "in_tuple")]),
(split_info, early_b0ref_wf, [("dwi_file", "inputnode.dwi_file"),
("bvec", "inputnode.in_bvec"),
("bval", "inputnode.in_bval")]),
(split_info, referencenode, [("dwi_file", "dwi_file"),
("metadata", "metadata")]),
(early_b0ref_wf, referencenode, [
("outputnode.dwi_reference", "dwi_reference"),
("outputnode.dwi_mask", "dwi_mask"),
("outputnode.gradients_rasb", "gradients_rasb"),
]),
])
fmap_estimation_wf = init_fmap_estimation_wf(subject_data["dwi"],
debug=config.execution.debug)
workflow.connect([
(referencenode, fmap_estimation_wf,
[("dwi_reference", "inputnode.dwi_reference"),
("dwi_mask", "inputnode.dwi_mask")]),
])
return workflow
