def distribute_master_mix(p300m: InstrumentContext, plates: [Labware],
tuberack: Labware, tipracks: [Labware]):
tubes = tuberack.wells()[0:4]
p300m.well_bottom_clearance.dispense = .02
tip_counter = 0
# to_remove is the number of wells to be removed from the final row. all prior rows will be assumed to be filled
to_remove = 11
to_remove = 12 - to_remove
# last_row is the index of the last row (rows() returns 2D list of rows by well)
last_row = 1
plate = plates[0]
# for plate in plates:
wells = plate.rows()[:2]
wells[last_row] = wells[last_row][:to_remove]
# for example, pop the last 8 in a row for a
# for plate in plates:
# # last_row is the index of the last row (rows() returns 2D list of rows by well)
#
# plate = plates[0]
# # for plate in plates:
# wells = plate.rows()[:2]
# wells[last_row] = wells[last_row][:to_remove]
group_counter = 0
tip_gen = next_tip(p300m.tip_racks, 1)
for tube in tubes:
p300m.well_bottom_clearance.aspirate = .1
print(wells)
wells = plate.rows()[:2 + group_counter]
group_counter += 2
wells[last_row] = wells[last_row][:to_remove]
for row in wells:
for well in row:
tip = next(tip_gen)
print(tip)
p300m.pick_up_tip(location=tip, presses=2, increment=.05)
print(tube, well)
p300m.aspirate(volume=100, location=tube)
p300m.dispense(volume=100, location=well)
p300m.well_bottom_clearance.aspirate = .02
p300m.mix(volume=60, repetitions=3, location=well)
p300m.drop_tip()
tip_counter += 1
def mix(pipette: InstrumentContext, n_mixes, vol, location):
if n_mixes == 0:
return
else:
pipette.mix(n_mixes, vol, location)