def run(protocol: protocol_api.ProtocolContext):
#from opentrons import simulate
#metadata = {'apiLevel': '2.8'}
#protocol = simulate.get_protocol_api('2.8')
#Set Column Location of Buffer in 12-well reservoir
#Load Column Location of Dye in 12-well reservoir
buffer_well = 'A1'
dye_well = 'A2'
#Labware
plate = protocol.load_labware(
'costar96flatbottomtransparent_96_wellplate_200ul', 1)
tiprack_1 = protocol.load_labware('opentrons_96_tiprack_300ul', 2)
reservoir = protocol.load_labware(
'brooksreservoirplate_12_wellplate_21000ul', 4)
#pipettes
p300 = protocol.load_instrument('p300_multi',
'left',
tip_racks=[tiprack_1])
protocol.max_speeds['Z'] = 100
#commands
#distributing pbs
p300.distribute(100,
reservoir.wells_by_name()[buffer_well],
plate.rows_by_name()['A'][1:])
#distributing dye
p300.pick_up_tip()
for i in range(2):
p300.transfer(100,
reservoir[dye_well],
plate['A' + str(i + 1)],
mix_before=(4, 75),
mix_after=(1, 75),
blow_out=True,
blowout_location='destination well',
new_tip='never')
#serial dilution
for i in range(9):
p300.transfer(100,
plate['A' + str(i + 2)],
plate['A' + str(i + 3)],
mix_before=(2, 75),
mix_after=(1, 75),
blow_out=True,
blowout_location='destination well',
new_tip='never')
p300.drop_tip()
for line in protocol.commands():
print(line)
