def test_plantcv_erode():
img = cv2.imread(os.path.join(TEST_DATA, TEST_INPUT_BINARY), -1)
device, erode_img = pcv.erode(img=img, kernel=5, i=1, device=0, debug=None)
# Assert that the output image has the dimensions of the input image
if all([i == j] for i, j in zip(np.shape(erode_img), TEST_BINARY_DIM)):
# Assert that the image is binary
if all([i == j] for i, j in zip(np.unique(erode_img), [0, 255])):
assert 1
else:
assert 0
else:
assert 0
def test_plantcv_erode():
img = cv2.imread(os.path.join(TEST_DATA, TEST_INPUT_BINARY), -1)
device, erode_img = pcv.erode(img=img, kernel=5, i=1, device=0, debug=None)
# Assert that the output image has the dimensions of the input image
if all([i == j] for i, j in zip(np.shape(erode_img), TEST_BINARY_DIM)):
# Assert that the image is binary
if all([i == j] for i, j in zip(np.unique(erode_img), [0, 255])):
assert 1
else:
assert 0
else:
assert 0
def main():
# Get options
args = options()
if args.debug:
print("Analyzing your image dude...")
# Read image
device = 0
img = cv2.imread(args.image, flags=0)
path, img_name = os.path.split(args.image)
# Read in image which is average of average of backgrounds
img_bkgrd = cv2.imread("bkgrd_ave_z2500.png", flags=0)
# NIR images for burnin2 are up-side down. This may be fixed in later experiments
img = ndimage.rotate(img, 180)
img_bkgrd = ndimage.rotate(img_bkgrd, 180)
# Subtract the image from the image background to make the plant more prominent
device, bkg_sub_img = pcv.image_subtract(img, img_bkgrd, device, args.debug)
if args.debug:
pcv.plot_hist(bkg_sub_img, "bkg_sub_img")
device, bkg_sub_thres_img = pcv.binary_threshold(bkg_sub_img, 145, 255, "dark", device, args.debug)
bkg_sub_thres_img = cv2.inRange(bkg_sub_img, 30, 220)
if args.debug:
cv2.imwrite("bkgrd_sub_thres.png", bkg_sub_thres_img)
# device, bkg_sub_thres_img = pcv.binary_threshold_2_sided(img_bkgrd, 50, 190, device, args.debug)
# if a region of interest is specified read it in
roi = cv2.imread(args.roi)
# Start by examining the distribution of pixel intensity values
if args.debug:
pcv.plot_hist(img, "hist_img")
# Will intensity transformation enhance your ability to isolate object of interest by thesholding?
device, he_img = pcv.HistEqualization(img, device, args.debug)
if args.debug:
pcv.plot_hist(he_img, "hist_img_he")
# Laplace filtering (identify edges based on 2nd derivative)
device, lp_img = pcv.laplace_filter(img, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(lp_img, "hist_lp")
# Lapacian image sharpening, this step will enhance the darkness of the edges detected
device, lp_shrp_img = pcv.image_subtract(img, lp_img, device, args.debug)
if args.debug:
pcv.plot_hist(lp_shrp_img, "hist_lp_shrp")
# Sobel filtering
# 1st derivative sobel filtering along horizontal axis, kernel = 1, unscaled)
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sbx_img, "hist_sbx")
# 1st derivative sobel filtering along vertical axis, kernel = 1, unscaled)
device, sby_img = pcv.sobel_filter(img, 0, 1, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sby_img, "hist_sby")
# Combine the effects of both x and y filters through matrix addition
# This will capture edges identified within each plane and emphesize edges found in both images
device, sb_img = pcv.image_add(sbx_img, sby_img, device, args.debug)
if args.debug:
pcv.plot_hist(sb_img, "hist_sb_comb_img")
# Use a lowpass (blurring) filter to smooth sobel image
device, mblur_img = pcv.median_blur(sb_img, 1, device, args.debug)
device, mblur_invert_img = pcv.invert(mblur_img, device, args.debug)
# combine the smoothed sobel image with the laplacian sharpened image
# combines the best features of both methods as described in "Digital Image Processing" by Gonzalez and Woods pg. 169
device, edge_shrp_img = pcv.image_add(mblur_invert_img, lp_shrp_img, device, args.debug)
if args.debug:
pcv.plot_hist(edge_shrp_img, "hist_edge_shrp_img")
# Perform thresholding to generate a binary image
device, tr_es_img = pcv.binary_threshold(edge_shrp_img, 145, 255, "dark", device, args.debug)
# Prepare a few small kernels for morphological filtering
kern = np.zeros((3, 3), dtype=np.uint8)
kern1 = np.copy(kern)
kern1[1, 1:3] = 1
kern2 = np.copy(kern)
kern2[1, 0:2] = 1
kern3 = np.copy(kern)
kern3[0:2, 1] = 1
kern4 = np.copy(kern)
kern4[1:3, 1] = 1
# Prepare a larger kernel for dilation
kern[1, 0:3] = 1
kern[0:3, 1] = 1
# Perform erosion with 4 small kernels
device, e1_img = pcv.erode(tr_es_img, kern1, 1, device, args.debug)
device, e2_img = pcv.erode(tr_es_img, kern2, 1, device, args.debug)
device, e3_img = pcv.erode(tr_es_img, kern3, 1, device, args.debug)
device, e4_img = pcv.erode(tr_es_img, kern4, 1, device, args.debug)
# Combine eroded images
device, c12_img = pcv.logical_or(e1_img, e2_img, device, args.debug)
device, c123_img = pcv.logical_or(c12_img, e3_img, device, args.debug)
device, c1234_img = pcv.logical_or(c123_img, e4_img, device, args.debug)
# Perform dilation
# device, dil_img = pcv.dilate(c1234_img, kern, 1, device, args.debug)
device, comb_img = pcv.logical_or(c1234_img, bkg_sub_thres_img, device, args.debug)
# Get masked image
# The dilated image may contain some pixels which are not plant
device, masked_erd = pcv.apply_mask(img, comb_img, "black", device, args.debug)
# device, masked_erd_dil = pcv.apply_mask(img, dil_img, 'black', device, args.debug)
# Need to remove the edges of the image, we did that by generating a set of rectangles to mask the edges
# img is (254 X 320)
# mask for the bottom of the image
device, box1_img, rect_contour1, hierarchy1 = pcv.rectangle_mask(img, (120, 184), (215, 252), device, args.debug)
# mask for the left side of the image
device, box2_img, rect_contour2, hierarchy2 = pcv.rectangle_mask(img, (1, 1), (85, 252), device, args.debug)
# mask for the right side of the image
device, box3_img, rect_contour3, hierarchy3 = pcv.rectangle_mask(img, (240, 1), (318, 252), device, args.debug)
# mask the edges
device, box4_img, rect_contour4, hierarchy4 = pcv.border_mask(img, (1, 1), (318, 252), device, args.debug)
# combine boxes to filter the edges and car out of the photo
device, bx12_img = pcv.logical_or(box1_img, box2_img, device, args.debug)
device, bx123_img = pcv.logical_or(bx12_img, box3_img, device, args.debug)
device, bx1234_img = pcv.logical_or(bx123_img, box4_img, device, args.debug)
device, inv_bx1234_img = pcv.invert(bx1234_img, device, args.debug)
# Make a ROI around the plant, include connected objects
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, edge_masked_img = pcv.apply_mask(masked_erd, inv_bx1234_img, "black", device, args.debug)
device, roi_img, roi_contour, roi_hierarchy = pcv.rectangle_mask(img, (120, 75), (200, 184), device, args.debug)
plant_objects, plant_hierarchy = cv2.findContours(edge_masked_img, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)
device, roi_objects, hierarchy5, kept_mask, obj_area = pcv.roi_objects(
img, "partial", roi_contour, roi_hierarchy, plant_objects, plant_hierarchy, device, args.debug
)
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, "black", device, args.debug)
rgb = cv2.cvtColor(img, cv2.COLOR_GRAY2RGB)
# Generate a binary to send to the analysis function
device, mask = pcv.binary_threshold(masked_img, 1, 255, "light", device, args.debug)
mask3d = np.copy(mask)
plant_objects_2, plant_hierarchy_2 = cv2.findContours(mask3d, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)
device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device, args.debug)
### Analysis ###
device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(
img, args.image, mask, 256, device, args.debug, args.outdir + "/" + img_name
)
device, shape_header, shape_data, ori_img = pcv.analyze_object(
rgb, args.image, o, m, device, args.debug, args.outdir + "/" + img_name
)
pcv.print_results(args.image, hist_header, hist_data)
pcv.print_results(args.image, shape_header, shape_data)
def main():
# Get options
args = options()
if args.debug:
print("Analyzing your image dude...")
# Read image
device = 0
img = cv2.imread(args.image, flags=0)
path, img_name = os.path.split(args.image)
# Read in image which is average of average of backgrounds
img_bkgrd = cv2.imread("bkgrd_ave_z3500.png", flags=0)
# NIR images for burnin2 are up-side down. This may be fixed in later experiments
img = ndimage.rotate(img, 180)
img_bkgrd = ndimage.rotate(img_bkgrd, 180)
# Subtract the image from the image background to make the plant more prominent
device, bkg_sub_img = pcv.image_subtract(img, img_bkgrd, device, args.debug)
if args.debug:
pcv.plot_hist(bkg_sub_img, 'bkg_sub_img')
device, bkg_sub_thres_img = pcv.binary_threshold(bkg_sub_img, 145, 255, 'dark', device, args.debug)
bkg_sub_thres_img = cv2.inRange(bkg_sub_img, 30, 220)
if args.debug:
cv2.imwrite('bkgrd_sub_thres.png', bkg_sub_thres_img)
#device, bkg_sub_thres_img = pcv.binary_threshold_2_sided(img_bkgrd, 50, 190, device, args.debug)
# if a region of interest is specified read it in
roi = cv2.imread(args.roi)
# Start by examining the distribution of pixel intensity values
if args.debug:
pcv.plot_hist(img, 'hist_img')
# Will intensity transformation enhance your ability to isolate object of interest by thesholding?
device, he_img = pcv.HistEqualization(img, device, args.debug)
if args.debug:
pcv.plot_hist(he_img, 'hist_img_he')
# Laplace filtering (identify edges based on 2nd derivative)
device, lp_img = pcv.laplace_filter(img, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(lp_img, 'hist_lp')
# Lapacian image sharpening, this step will enhance the darkness of the edges detected
device, lp_shrp_img = pcv.image_subtract(img, lp_img, device, args.debug)
if args.debug:
pcv.plot_hist(lp_shrp_img, 'hist_lp_shrp')
# Sobel filtering
# 1st derivative sobel filtering along horizontal axis, kernel = 1, unscaled)
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sbx_img, 'hist_sbx')
# 1st derivative sobel filtering along vertical axis, kernel = 1, unscaled)
device, sby_img = pcv.sobel_filter(img, 0, 1, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sby_img, 'hist_sby')
# Combine the effects of both x and y filters through matrix addition
# This will capture edges identified within each plane and emphesize edges found in both images
device, sb_img = pcv.image_add(sbx_img, sby_img, device, args.debug)
if args.debug:
pcv.plot_hist(sb_img, 'hist_sb_comb_img')
# Use a lowpass (blurring) filter to smooth sobel image
device, mblur_img = pcv.median_blur(sb_img, 1, device, args.debug)
device, mblur_invert_img = pcv.invert(mblur_img, device, args.debug)
# combine the smoothed sobel image with the laplacian sharpened image
# combines the best features of both methods as described in "Digital Image Processing" by Gonzalez and Woods pg. 169
device, edge_shrp_img = pcv.image_add(mblur_invert_img, lp_shrp_img, device, args.debug)
if args.debug:
pcv.plot_hist(edge_shrp_img, 'hist_edge_shrp_img')
# Perform thresholding to generate a binary image
device, tr_es_img = pcv.binary_threshold(edge_shrp_img, 145, 255, 'dark', device, args.debug)
# Prepare a few small kernels for morphological filtering
kern = np.zeros((3,3), dtype=np.uint8)
kern1 = np.copy(kern)
kern1[1,1:3]=1
kern2 = np.copy(kern)
kern2[1,0:2]=1
kern3 = np.copy(kern)
kern3[0:2,1]=1
kern4 = np.copy(kern)
kern4[1:3,1]=1
# Prepare a larger kernel for dilation
kern[1,0:3]=1
kern[0:3,1]=1
# Perform erosion with 4 small kernels
device, e1_img = pcv.erode(tr_es_img, kern1, 1, device, args.debug)
device, e2_img = pcv.erode(tr_es_img, kern2, 1, device, args.debug)
device, e3_img = pcv.erode(tr_es_img, kern3, 1, device, args.debug)
device, e4_img = pcv.erode(tr_es_img, kern4, 1, device, args.debug)
# Combine eroded images
device, c12_img = pcv.logical_or(e1_img, e2_img, device, args.debug)
device, c123_img = pcv.logical_or(c12_img, e3_img, device, args.debug)
device, c1234_img = pcv.logical_or(c123_img, e4_img, device, args.debug)
# Perform dilation
# device, dil_img = pcv.dilate(c1234_img, kern, 1, device, args.debug)
device, comb_img = pcv.logical_or(c1234_img, bkg_sub_thres_img, device, args.debug)
# Get masked image
# The dilated image may contain some pixels which are not plant
device, masked_erd = pcv.apply_mask(img, comb_img, 'black', device, args.debug)
# device, masked_erd_dil = pcv.apply_mask(img, dil_img, 'black', device, args.debug)
# Need to remove the edges of the image, we did that by generating a set of rectangles to mask the edges
# img is (254 X 320)
# mask for the bottom of the image
device, box1_img, rect_contour1, hierarchy1 = pcv.rectangle_mask(img, (100,210), (230,252), device, args.debug)
# mask for the left side of the image
device, box2_img, rect_contour2, hierarchy2 = pcv.rectangle_mask(img, (1,1), (85,252), device, args.debug)
# mask for the right side of the image
device, box3_img, rect_contour3, hierarchy3 = pcv.rectangle_mask(img, (240,1), (318,252), device, args.debug)
# mask the edges
device, box4_img, rect_contour4, hierarchy4 = pcv.border_mask(img, (1,1), (318,252), device, args.debug)
# combine boxes to filter the edges and car out of the photo
device, bx12_img = pcv.logical_or(box1_img, box2_img, device, args.debug)
device, bx123_img = pcv.logical_or(bx12_img, box3_img, device, args.debug)
device, bx1234_img = pcv.logical_or(bx123_img, box4_img, device, args.debug)
device, inv_bx1234_img = pcv.invert(bx1234_img, device, args.debug)
# Make a ROI around the plant, include connected objects
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, edge_masked_img = pcv.apply_mask(masked_erd, inv_bx1234_img, 'black', device, args.debug)
device, roi_img, roi_contour, roi_hierarchy = pcv.rectangle_mask(img, (100,75), (220,208), device, args.debug)
plant_objects, plant_hierarchy = cv2.findContours(edge_masked_img,cv2.RETR_TREE,cv2.CHAIN_APPROX_NONE)
device, roi_objects, hierarchy5, kept_mask, obj_area = pcv.roi_objects(img, 'partial', roi_contour, roi_hierarchy, plant_objects, plant_hierarchy, device, args.debug)
# Apply the box mask to the image
# device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, 'black', device, args.debug)
rgb = cv2.cvtColor(img,cv2.COLOR_GRAY2RGB)
# Generate a binary to send to the analysis function
device, mask = pcv.binary_threshold(masked_img, 1, 255, 'light', device, args.debug)
mask3d = np.copy(mask)
plant_objects_2, plant_hierarchy_2 = cv2.findContours(mask3d,cv2.RETR_TREE,cv2.CHAIN_APPROX_NONE)
device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device, args.debug)
### Analysis ###
device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(img, args.image, mask, 256, device, args.debug, args.outdir + '/' + img_name)
device, shape_header, shape_data, ori_img = pcv.analyze_object(rgb, args.image, o, m, device, args.debug, args.outdir + '/' + img_name)
pcv.print_results(args.image, hist_header, hist_data)
pcv.print_results(args.image, shape_header, shape_data)
def get_feature(img):
print("step one")
"""
Step one: Background forground substraction
"""
# Get options
args = options()
debug = args.debug
# Read image
filename = args.result
# img, path, filename = pcv.readimage(args.image)
# Pipeline step
device = 0
device, resize_img = pcv.resize(img, 0.4, 0.4, device, debug)
# Classify the pixels as plant or background
device, mask_img = pcv.naive_bayes_classifier(
resize_img,
pdf_file=
"/home/matthijs/PycharmProjects/SMR1/src/vision/ML_background/Trained_models/model_3/naive_bayes_pdfs.txt",
device=0,
debug='print')
# Median Filter
device, blur = pcv.median_blur(mask_img.get('plant'), 5, device, debug)
print("step two")
"""
Step one: Identifiy the objects, extract and filter the objects
"""
# Identify objects
device, id_objects, obj_hierarchy = pcv.find_objects(resize_img,
blur,
device,
debug=None)
# Define ROI
device, roi1, roi_hierarchy = pcv.define_roi(resize_img,
'rectangle',
device,
roi=True,
roi_input='default',
debug=True,
adjust=True,
x_adj=50,
y_adj=10,
w_adj=-100,
h_adj=0)
# Decide which objects to keep
device, roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(
resize_img, 'cutto', roi1, roi_hierarchy, id_objects, obj_hierarchy,
device, debug)
# print(roi_objects[0])
# cv2.drawContours(resize_img, [roi_objects[0]], 0, (0, 255, 0), 3)
# cv2.imshow("img",resize_img)
# cv2.waitKey(0)
area_oud = 0
i = 0
index = 0
object_list = []
# a = np.array([[hierarchy3[0][0]]])
hierarchy = []
for cnt in roi_objects:
area = cv2.contourArea(cnt)
M = cv2.moments(cnt)
if M["m10"] or M["m01"]:
cX = int(M["m10"] / M["m00"])
cY = int(M["m01"] / M["m00"])
# check if the location of the contour is between the constrains
if cX > 200 and cX < 500 and cY > 25 and cY < 400:
# cv2.circle(resize_img, (cX, cY), 5, (255, 0, 255), thickness=1, lineType=1, shift=0)
# check if the size of the contour is bigger than 250
if area > 450:
obj = np.vstack(roi_objects)
object_list.append(roi_objects[i])
hierarchy.append(hierarchy3[0][i])
print(i)
i = i + 1
a = np.array([hierarchy])
# a = [[[-1,-1,-1,-1][-1,-1,-1,-1][-1,-1,-1,-1]]]
# Object combine kept objects
# device, obj, mask_2 = pcv.object_composition(resize_img, object_list, a, device, debug)
mask_contours = np.zeros(resize_img.shape, np.uint8)
cv2.drawContours(mask_contours, object_list, -1, (255, 255, 255), -1)
gray_image = cv2.cvtColor(mask_contours, cv2.COLOR_BGR2GRAY)
ret, mask_contours = cv2.threshold(gray_image, 127, 255, cv2.THRESH_BINARY)
# Identify objects
device, id_objects, obj_hierarchy = pcv.find_objects(resize_img,
mask_contours,
device,
debug=None)
# Decide which objects to keep
device, roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(
resize_img,
'cutto',
roi1,
roi_hierarchy,
id_objects,
obj_hierarchy,
device,
debug=None)
# Object combine kept objects
device, obj, mask = pcv.object_composition(resize_img,
roi_objects,
hierarchy3,
device,
debug=None)
############### Analysis ################
masked = mask.copy()
outfile = False
if args.writeimg == True:
outfile = args.outdir + "/" + filename
print("step three")
"""
Step three: Calculate all the features
"""
# Find shape properties, output shape image (optional)
device, shape_header, shape_data, shape_img = pcv.analyze_object(
resize_img, args.image, obj, mask, device, debug, filename="/file")
print(shape_img)
# Shape properties relative to user boundary line (optional)
device, boundary_header, boundary_data, boundary_img1 = pcv.analyze_bound(
resize_img, args.image, obj, mask, 1680, device)
# Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
device, color_header, color_data, color_img = pcv.analyze_color(
resize_img, args.image, kept_mask, 256, device, debug, 'all', 'v',
'img', 300)
maks_watershed = mask.copy()
kernel = np.zeros((5, 5), dtype=np.uint8)
device, mask_watershed, = pcv.erode(maks_watershed, 5, 1, device, debug)
device, watershed_header, watershed_data, analysis_images = pcv.watershed_segmentation(
device, resize_img, mask, 50, './examples', debug)
device, list_of_acute_points = pcv.acute_vertex(obj, 30, 60, 10,
resize_img, device, debug)
device, top, bottom, center_v = pcv.x_axis_pseudolandmarks(
obj, mask, resize_img, device, debug)
device, left, right, center_h = pcv.y_axis_pseudolandmarks(
obj, mask, resize_img, device, debug)
device, points_rescaled, centroid_rescaled, bottomline_rescaled = pcv.scale_features(
obj, mask, list_of_acute_points, 225, device, debug)
# Identify acute vertices (tip points) of an object
# Results in set of point values that may indicate tip points
device, vert_ave_c, hori_ave_c, euc_ave_c, ang_ave_c, vert_ave_b, hori_ave_b, euc_ave_b, ang_ave_b = pcv.landmark_reference_pt_dist(
points_rescaled, centroid_rescaled, bottomline_rescaled, device, debug)
landmark_header = [
'HEADER_LANDMARK', 'tip_points', 'tip_points_r', 'centroid_r',
'baseline_r', 'tip_number', 'vert_ave_c', 'hori_ave_c', 'euc_ave_c',
'ang_ave_c', 'vert_ave_b', 'hori_ave_b', 'euc_ave_b', 'ang_ave_b',
'left_lmk', 'right_lmk', 'center_h_lmk', 'left_lmk_r', 'right_lmk_r',
'center_h_lmk_r', 'top_lmk', 'bottom_lmk', 'center_v_lmk', 'top_lmk_r',
'bottom_lmk_r', 'center_v_lmk_r'
]
landmark_data = [
'LANDMARK_DATA', 0, 0, 0, 0,
len(list_of_acute_points), vert_ave_c, hori_ave_c, euc_ave_c,
ang_ave_c, vert_ave_b, hori_ave_b, euc_ave_b, ang_ave_b, 0, 0, 0, 0, 0,
0, 0, 0, 0, 0, 0, 0
]
shape_data_train = list(shape_data)
shape_data_train.pop(0)
shape_data_train.pop(10)
watershed_data_train = list(watershed_data)
watershed_data_train.pop(0)
landmark_data_train = [
len(list_of_acute_points), vert_ave_c, hori_ave_c, euc_ave_c,
ang_ave_c, vert_ave_b, hori_ave_b, euc_ave_b, ang_ave_b
]
X = shape_data_train + watershed_data_train + landmark_data_train
print("len X", len(X))
print(X)
# Write shape and color data to results fil
result = open(args.result, "a")
result.write('\t'.join(map(str, shape_header)))
result.write("\n")
result.write('\t'.join(map(str, shape_data)))
result.write("\n")
result.write('\t'.join(map(str, watershed_header)))
result.write("\n")
result.write('\t'.join(map(str, watershed_data)))
result.write("\n")
result.write('\t'.join(map(str, landmark_header)))
result.write("\n")
result.write('\t'.join(map(str, landmark_data)))
result.write("\n")
for row in shape_img:
result.write('\t'.join(map(str, row)))
result.write("\n")
result.write('\t'.join(map(str, color_header)))
result.write("\n")
result.write('\t'.join(map(str, color_data)))
result.write("\n")
for row in color_img:
result.write('\t'.join(map(str, row)))
result.write("\n")
result.close()
print("done")
print(shape_img)
return X, shape_img, masked
args = options()
debug = args.debug
device = 0
img = cv2.imread("/home/matthijs/Downloads/tomaat_3.jpg")
img_2 = cv2.imread("/home/matthijs/Downloads/tomaat_3.jpg", 0)
cimg = cv2.cvtColor(img_2, cv2.COLOR_BAYER_BG2BGR)
device, s = pcv.rgb2gray_hsv(img, 's', device)
device, a = pcv.rgb2gray_lab(img, 'a', device, debug) # looks most promissing
device, a_thresh = pcv.binary_threshold(a, 135, 255, 'light', device, debug)
device, a_mblur = pcv.median_blur(a_thresh, 5, device, debug)
kernel = np.zeros((3, 3), dtype=np.uint8)
device, mask_watershed, = pcv.erode(a_mblur, 5, 1, device, debug)
circles = cv2.HoughCircles(img_2,
cv2.cv.CV_HOUGH_GRADIENT,
1,
30,
param1=50,
param2=20,
minRadius=130,
maxRadius=220)
print("watt?")
circles = np.uint16(np.around(circles))
for i in circles[0, :]:
cv2.circle(cimg, (i[0], i[1]), i[2], (0, 255, 0), 2)
def main():
# obtiene opciones de imagen
args = options()
#LINEA 22
if args.debug:
print("Debug mode turned on...")
# lee la imagen el flags=0 indica que se espera una imagen a escala de grises
img = cv2.imread(args.image, flags=0)
# cv2.imshow("imagen original",img)
# Get directory path and image name from command line arguments
path, img_name = os.path.split(args.image)
#LINEA 30
# Read in image which is the pixelwise average of background images
img_bkgrd = cv2.imread("background_average.jpg", flags=0)
#cv2.imshow("ventana del fondo",img_bkgrd)
# paso del procesamiento de imagenes
device = 0
######hasta qui bien
#linea 37
# Restar la imagen de fondo de la imagen con la planta.
device, bkg_sub_img = pcv.image_subtract(img, img_bkgrd, device,
args.debug)
#cv2.imshow("imagen resta",bkg_sub_img)
# Threshold the image of interest using the two-sided cv2.inRange function (keep what is between 50-190)
bkg_sub_thres_img = cv2.inRange(bkg_sub_img, 50, 190)
if args.debug:
cv2.imwrite('bkgrd_sub_thres.png', bkg_sub_thres_img)
#hasta qui todo bien
#linea 46
# Filtrado de Laplace (identificar bordes basados en la derivada 2)
device, lp_img = pcv.laplace_filter(img, 1, 1, device, args.debug)
#cv2.imshow("imagen de filtrado",lp_img)
if args.debug:
pcv.plot_hist(lp_img, 'histograma_lp')
# Lapacian image sharpening, this step will enhance the darkness of the edges detected
device, lp_shrp_img = pcv.image_subtract(img, lp_img, device, args.debug)
#cv2.imshow("imagen de borde lapacian",lp_shrp_img)
if args.debug:
pcv.plot_hist(lp_shrp_img, 'histograma_lp_shrp')
#hasta aqui todo bien linea 58
# Sobel filtering-filtrado de sobel
# 1ª derivada filtrado sobel a lo largo del eje horizontal, núcleo = 1, sin escala)
""" segun esta masl son siete,kito scale y me kedo con apertura k,chekar sobel en docs
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, 1, device, args.debug)
"""
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, device, args.debug)
#cv2.imshow("imagen sobel-eje horizontal",sbx_img)
if args.debug:
pcv.plot_hist(sbx_img, 'histograma_sbx')
# Filtrado de la primera derivada sobel a lo largo del eje vertical, núcleo = 1, sin escala)
device, sby_img = pcv.sobel_filter(img, 0, 1, 1, device, args.debug)
#cv2.imshow("imagen sobel-ejevertical",sby_img)
if args.debug:
pcv.plot_hist(sby_img, 'histograma_sby')
# Combina los efectos de ambos filtros x e y mediante la suma de matrizes
# Esto captura los bordes identificados dentro de cada plano y enfatiza los bordes encontrados en ambas imágenes
device, sb_img = pcv.image_add(sbx_img, sby_img, device, args.debug)
#cv2.imshow("imagen suma de sobel",sb_img)
if args.debug:
pcv.plot_hist(sb_img, 'histograma_sb_comb_img')
#hasta aqui todo bien linea 82
# usar filtro pasa bajo blur para suavizar la imagen de sobel
device, mblur_img = pcv.median_blur(sb_img, 1, device, args.debug)
#cv2.imshow("imagen blur",mblur_img)
device, mblur_invert_img = pcv.invert(mblur_img, device, args.debug)
#cv2.imshow("imagen blur-invertido",mblur_invert_img)
# Combinar la imagen suavizada del sobel con la imagen afilada del laplaciano
# combines the best features of both methods as described in "Digital Image Processing" by Gonzalez and Woods pg. 169
#Combina las mejores características de ambos métodos como se describe en "Digital Image Processing" por González y Woods pág. 169
device, edge_shrp_img = pcv.image_add(mblur_invert_img, lp_shrp_img,
device, args.debug)
#cv2.imshow("imagen-combinacion-sobel-laplacian",mblur_img)
if args.debug:
pcv.plot_hist(edge_shrp_img, 'hist_edge_shrp_img')
# Realizar el umbral para generar una imagen binaria
device, tr_es_img = pcv.binary_threshold(edge_shrp_img, 125, 255, 'dark',
device, args.debug)
#cv2.imshow("imagen binaria de combinacion",tr_es_img)
#hasta aqui todo bien linea 99
# Prepare a few small kernels for morphological filtering
#prepara nucleos pequeños para un filtrado moorfologico
kern = np.zeros((3, 3), dtype=np.uint8)
kern1 = np.copy(kern)
kern1[1, 1:3] = 1
kern2 = np.copy(kern)
kern2[1, 0:2] = 1
kern3 = np.copy(kern)
kern3[0:2, 1] = 1
kern4 = np.copy(kern)
kern4[1:3, 1] = 1
# prepara un nucleo grande para la dilatacion
kern[1, 0:3] = 1
kern[0:3, 1] = 1
# Perform erosion with 4 small kernels
device, e1_img = pcv.erode(tr_es_img, 1, 1, device, args.debug)
#cv2.imshow("erosion 1",e1_img)
device, e2_img = pcv.erode(tr_es_img, 1, 1, device, args.debug)
#cv2.imshow("erosion 2",e2_img)
device, e3_img = pcv.erode(tr_es_img, 1, 1, device, args.debug)
#cv2.imshow("erosion 3",e3_img)
device, e4_img = pcv.erode(tr_es_img, 1, 1, device, args.debug)
#cv2.imshow("erosion 4",e4_img)
# Combine eroded images
device, c12_img = pcv.logical_or(e1_img, e2_img, device, args.debug)
#cv2.imshow("c12",c12_img)
device, c123_img = pcv.logical_or(c12_img, e3_img, device, args.debug)
#cv2.imshow("c123",c123_img)
device, c1234_img = pcv.logical_or(c123_img, e4_img, device, args.debug)
#cv2.imshow("c1234",c1234_img)
# Bring the two object identification approaches together.
# Using a logical OR combine object identified by background subtraction and the object identified by derivative filter.
device, comb_img = pcv.logical_or(c1234_img, bkg_sub_thres_img, device,
args.debug)
#cv2.imshow("comb_img",comb_img)
# Get masked image, Essentially identify pixels corresponding to plant and keep those.
device, masked_erd = pcv.apply_mask(img, comb_img, 'black', device,
args.debug)
#cv2.imshow("masked_erd",masked_erd)
#cv2.imshow("imagen original chkar",img)
# Need to remove the edges of the image, we did that by generating a set of rectangles to mask the edges
# img is (254 X 320)
# mask for the bottom of the image
device, im2, box1_img, rect_contour1, hierarchy1 = pcv.rectangle_mask(
img, (120, 184), (215, 252), device, args.debug, color='white')
#cv2.imshow("im2",box1_img)
# mask for the left side of the image
device, im3, box2_img, rect_contour2, hierarchy2 = pcv.rectangle_mask(
img, (1, 1), (85, 252), device, args.debug, color='white')
#cv2.imshow("im3",box2_img)
# mask for the right side of the image
device, im4, box3_img, rect_contour3, hierarchy3 = pcv.rectangle_mask(
img, (240, 1), (318, 252), device, args.debug, color='white')
#cv2.imshow("im4",box3_img)
# mask the edges
device, im5, box4_img, rect_contour4, hierarchy4 = pcv.rectangle_mask(
img, (1, 1), (318, 252), device, args.debug)
#cv2.imshow("im5",box4_img)
# combine boxes to filter the edges and car out of the photo
device, bx12_img = pcv.logical_or(box1_img, box2_img, device, args.debug)
device, bx123_img = pcv.logical_or(bx12_img, box3_img, device, args.debug)
device, bx1234_img = pcv.logical_or(bx123_img, box4_img, device,
args.debug)
#cv2.imshow("combinacion logica or",bx1234_img)
# invert this mask and then apply it the masked image.
device, inv_bx1234_img = pcv.invert(bx1234_img, device, args.debug)
# cv2.imshow("combinacion logica or invertida",inv_bx1234_img)
device, edge_masked_img = pcv.apply_mask(masked_erd, inv_bx1234_img,
'black', device, args.debug)
# cv2.imshow("edge_masked_img",edge_masked_img)
# assign the coordinates of an area of interest (rectangle around the area you expect the plant to be in)
device, im6, roi_img, roi_contour, roi_hierarchy = pcv.rectangle_mask(
img, (120, 75), (200, 184), device, args.debug)
#cv2.imshow("im6",roi_img)
# get the coordinates of the plant from the masked object
plant_objects, plant_hierarchy = cv2.findContours(edge_masked_img,
cv2.RETR_TREE,
cv2.CHAIN_APPROX_NONE)
# Obtain the coordinates of the plant object which are partially within the area of interest
device, roi_objects, hierarchy5, kept_mask, obj_area = pcv.roi_objects(
img, 'partial', roi_contour, roi_hierarchy, plant_objects,
plant_hierarchy, device, args.debug)
# Apply the box mask to the image to ensure no background
device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, 'black',
device, args.debug)
#cv2.imshow("mascara final",masked_img)
#/////////////////////////////////////////////////////////////
#device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, 'black', device, args.debug)
rgb = cv2.cvtColor(img, cv2.COLOR_GRAY2RGB)
#cv2.imshow("rgb",rgb)
# Generate a binary to send to the analysis function
device, mask = pcv.binary_threshold(masked_img, 1, 255, 'light', device,
args.debug)
#cv2.imshow("mask",mask)
mask3d = np.copy(mask)
plant_objects_2, plant_hierarchy_2 = cv2.findContours(
mask3d, cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)
device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device,
args.debug)
# Get final masked image
device, masked_img = pcv.apply_mask(kept_mask, inv_bx1234_img, 'black',
device, args.debug)
#cv2.imshow("maskara final2",masked_img)
################### copia lo de arriba esta mal el tutorial
# Obtain a 3 dimensional representation of this grayscale image (for pseudocoloring)
#rgb = cv2.cvtColor(img,cv2.COLOR_GRAY2RGB)
# Generate a binary to send to the analysis function
#device, mask = pcv.binary_threshold(masked_img, 1, 255, 'light', device, args.debug)
# Make a copy of this mask for pseudocoloring
#mask3d = np.copy(mask)
# Extract coordinates of plant for pseudocoloring of plant
#plant_objects_2, plant_hierarchy_2 = cv2.findContours(mask3d,cv2.RETR_TREE,cv2.CHAIN_APPROX_NONE)
#device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device, args.debug)
# Extract coordinates of plant for pseudocoloring of plant
#plant_objects_2, plant_hierarchy_2 = cv2.findContours(mask3d,cv2.RETR_TREE,cv2.CHAIN_APPROX_NONE)
#device, o, m = pcv.object_composition(rgb, roi_objects, hierarchy5, device, args.debug)
####################################
#######################
### Analysis ###
# Perform signal analysis
#################pruebas de que esta masl el tutorial""""""""""""""""
#ols=type(args.image)
#print ols
##############pruebas de que no agarro device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(img, args.image, mask, 256, device, args.debug, args.outdir + '/' + img_name)
#print(args.outdir+'/'+img_name)
#print(args.debug)
#al final si salio se agrego lo qyue esta debug= and filename=
##################################################### debug me marca True por ello puse pritn de mas
#device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(img, rgb, mask, 256, device, debug='print', filename=False)
device, hist_header, hist_data, h_norm = pcv.analyze_NIR_intensity(
img,
rgb,
mask,
256,
device,
debug=args.debug,
filename=args.outdir + '/' + img_name)
# Perform shape analysis
device, shape_header, shape_data, ori_img = pcv.analyze_object(
rgb,
args.image,
o,
m,
device,
debug=args.debug,
filename=args.outdir + '/' + img_name)
# Print the results to STDOUT
pcv.print_results(args.image, hist_header, hist_data)
pcv.print_results(args.image, shape_header, shape_data)
cv2.waitKey()
cv2.destroyAllWdindows()
def main():
# Get options
args = options()
if args.debug:
print("Analyzing your image dude...")
# Read image
img = cv2.imread(args.image, flags=0)
# if a region of interest is specified read it in
roi = cv2.imread(args.roi)
# Pipeline step
device = 0
# Start by examining the distribution of pixel intensity values
if args.debug:
pcv.plot_hist(img, 'hist_img')
# Will intensity transformation enhance your ability to isolate object of interest by thesholding?
device, he_img = pcv.HistEqualization(img, device, args.debug)
if args.debug:
pcv.plot_hist(he_img, 'hist_img_he')
# Laplace filtering (identify edges based on 2nd derivative)
device, lp_img = pcv.laplace_filter(img, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(lp_img, 'hist_lp')
# Lapacian image sharpening, this step will enhance the darkness of the edges detected
device, lp_shrp_img = pcv.image_subtract(img, lp_img, device, args.debug)
if args.debug:
pcv.plot_hist(lp_shrp_img, 'hist_lp_shrp')
# Sobel filtering
# 1st derivative sobel filtering along horizontal axis, kernel = 1, unscaled)
device, sbx_img = pcv.sobel_filter(img, 1, 0, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sbx_img, 'hist_sbx')
# 1st derivative sobel filtering along vertical axis, kernel = 1, unscaled)
device, sby_img = pcv.sobel_filter(img, 0, 1, 1, 1, device, args.debug)
if args.debug:
pcv.plot_hist(sby_img, 'hist_sby')
# Combine the effects of both x and y filters through matrix addition
# This will capture edges identified within each plane and emphesize edges found in both images
device, sb_img = pcv.image_add(sbx_img, sby_img, device, args.debug)
if args.debug:
pcv.plot_hist(sb_img, 'hist_sb_comb_img')
# Use a lowpass (blurring) filter to smooth sobel image
device, mblur_img = pcv.median_blur(sb_img, 1, device, args.debug)
device, mblur_invert_img = pcv.invert(mblur_img, device, args.debug)
# combine the smoothed sobel image with the laplacian sharpened image
# combines the best features of both methods as described in "Digital Image Processing" by Gonzalez and Woods pg. 169
device, edge_shrp_img = pcv.image_add(mblur_invert_img, lp_shrp_img, device, args.debug)
if args.debug:
pcv.plot_hist(edge_shrp_img, 'hist_edge_shrp_img')
# Perform thresholding to generate a binary image
device, tr_es_img = pcv.binary_threshold(edge_shrp_img, 145, 255, 'dark', device, args.debug)
# Prepare a few small kernels for morphological filtering
kern = np.zeros((3,3), dtype=np.uint8)
kern1 = np.copy(kern)
kern1[1,1:3]=1
kern2 = np.copy(kern)
kern2[1,0:2]=1
kern3 = np.copy(kern)
kern3[0:2,1]=1
kern4 = np.copy(kern)
kern4[1:3,1]=1
# Prepare a larger kernel for dilation
kern[1,0:3]=1
kern[0:3,1]=1
# Perform erosion with 4 small kernels
device, e1_img = pcv.erode(tr_es_img, kern1, 1, device, args.debug)
device, e2_img = pcv.erode(tr_es_img, kern2, 1, device, args.debug)
device, e3_img = pcv.erode(tr_es_img, kern3, 1, device, args.debug)
device, e4_img = pcv.erode(tr_es_img, kern4, 1, device, args.debug)
# Combine eroded images
device, c12_img = pcv.logical_or(e1_img, e2_img, device, args.debug)
device, c123_img = pcv.logical_or(c12_img, e3_img, device, args.debug)
device, c1234_img = pcv.logical_or(c123_img, e4_img, device, args.debug)
# Perform dilation
device, dil_img = pcv.dilate(c1234_img, kern, 1, device, args.debug)
# Get masked image
# The dilated image may contain some pixels which are not plant
device, masked_erd = pcv.apply_mask(img, c1234_img, 'black', device, args.debug)
device, masked_erd_dil = pcv.apply_mask(img, dil_img, 'black', device, args.debug)
# Need to remove the edges of the image, we did that by generating a set of rectangles to mask the edges
# img is (254 X 320)
device, box1_img, rect_contour1, hierarchy1 = pcv.rectangle_mask(img, (1,1), (64,252), device, args.debug)
device, box2_img, rect_contour2, hierarchy2 = pcv.rectangle_mask(img, (256,1), (318,252), device, args.debug)
device, box3_img, rect_contour3, hierarchy3 = pcv.rectangle_mask(img, (1,184), (318,252), device, args.debug)
device, box4_img, rect_contour4, hierarchy4 = pcv.border_mask(img, (1,1), (318,252), device, args.debug)
# combine boxes to filter the edges and car out of the photo
device, bx12_img = pcv.logical_or(box1_img, box2_img, device, args.debug)
device, bx123_img = pcv.logical_or(bx12_img, box3_img, device, args.debug)
device, bx1234_img = pcv.logical_or(bx123_img, box4_img, device, args.debug)
device, inv_bx1234_img = pcv.invert(bx1234_img, device, args.debug)
# Apply the box mask to the image
device, masked_img = pcv.apply_mask(masked_erd_dil, inv_bx1234_img, 'black', device, args.debug)
# Generate a binary to send to the analysis function
device, mask = pcv.binary_threshold(masked_img, 1, 255, 'light', device, args.debug)
pcv.analyze_NIR_intensity(img, args.image, mask, 256, device, args.debug, 'example')
def main():
# Parse command-line options
args = options()
device = 0
# Open output file
out = open(args.outfile, "w")
# Open the image file
img, path, fname = pcv.readimage(filename=args.image, debug=args.debug)
# Classify healthy and unhealthy plant pixels
device, masks = pcv.naive_bayes_classifier(img=img,
pdf_file=args.pdfs,
device=device)
# Use the identified blue mesh area to build a mask for the pot area
# First errode the blue mesh region to remove background
device, mesh_errode = pcv.erode(img=masks["Background_Blue"],
kernel=9,
i=3,
device=device,
debug=args.debug)
# Define a region of interest for blue mesh contours
device, pot_roi, pot_hierarchy = pcv.define_roi(img=img,
shape='rectangle',
device=device,
roi=None,
roi_input='default',
debug=args.debug,
adjust=True,
x_adj=0,
y_adj=500,
w_adj=0,
h_adj=-650)
# Find blue mesh contours
device, mesh_objects, mesh_hierarchy = pcv.find_objects(img=img,
mask=mesh_errode,
device=device,
debug=args.debug)
# Keep blue mesh contours in the region of interest
device, kept_mesh_objs, kept_mesh_hierarchy, kept_mask_mesh, _ = pcv.roi_objects(
img=img,
roi_type='partial',
roi_contour=pot_roi,
roi_hierarchy=pot_hierarchy,
object_contour=mesh_objects,
obj_hierarchy=mesh_hierarchy,
device=device,
debug=args.debug)
# Flatten the blue mesh contours into a single object
device, mesh_flattened, mesh_mask = pcv.object_composition(
img=img,
contours=kept_mesh_objs,
hierarchy=kept_mesh_hierarchy,
device=device,
debug=args.debug)
# Initialize a pot mask
pot_mask = np.zeros(np.shape(masks["Background_Blue"]), dtype=np.uint8)
# Find the minimum bounding rectangle for the blue mesh region
rect = cv2.minAreaRect(mesh_flattened)
# Create a contour for the minimum bounding box
box = cv2.boxPoints(rect)
box = np.int0(box)
# Create a mask from the bounding box contour
cv2.drawContours(pot_mask, [box], 0, (255), -1)
# If the bounding box area is too small then the plant has likely occluded too much of the pot for us to use this
# as a marker for the pot area
if np.sum(pot_mask) / 255 < 2900000:
print(np.sum(pot_mask) / 255)
# Create a new pot mask
pot_mask = np.zeros(np.shape(masks["Background_Blue"]), dtype=np.uint8)
# Set the mask area to the ROI area
box = np.array([[0, 500], [0, 2806], [2304, 2806], [2304, 500]])
cv2.drawContours(pot_mask, [box], 0, (255), -1)
# Dialate the blue mesh area to include the ridge of the pot
device, pot_mask_dilated = pcv.dilate(img=pot_mask,
kernel=3,
i=60,
device=device,
debug=args.debug)
# Mask the healthy mask
device, healthy_masked = pcv.apply_mask(img=cv2.merge(
[masks["Healthy"], masks["Healthy"], masks["Healthy"]]),
mask=pot_mask_dilated,
mask_color="black",
device=device,
debug=args.debug)
# Mask the unhealthy mask
device, unhealthy_masked = pcv.apply_mask(img=cv2.merge(
[masks["Unhealthy"], masks["Unhealthy"], masks["Unhealthy"]]),
mask=pot_mask_dilated,
mask_color="black",
device=device,
debug=args.debug)
# Convert the masks back to binary
healthy_masked, _, _ = cv2.split(healthy_masked)
unhealthy_masked, _, _ = cv2.split(unhealthy_masked)
# Fill small objects
device, fill_image_healthy = pcv.fill(img=np.copy(healthy_masked),
mask=np.copy(healthy_masked),
size=300,
device=device,
debug=args.debug)
device, fill_image_unhealthy = pcv.fill(img=np.copy(unhealthy_masked),
mask=np.copy(unhealthy_masked),
size=1000,
device=device,
debug=args.debug)
# Define a region of interest
device, roi1, roi_hierarchy = pcv.define_roi(img=img,
shape='rectangle',
device=device,
roi=None,
roi_input='default',
debug=args.debug,
adjust=True,
x_adj=450,
y_adj=1000,
w_adj=-400,
h_adj=-1000)
# Filter objects that overlap the ROI
device, id_objects, obj_hierarchy_healthy = pcv.find_objects(
img=img, mask=fill_image_healthy, device=device, debug=args.debug)
device, _, _, kept_mask_healthy, _ = pcv.roi_objects(
img=img,
roi_type='partial',
roi_contour=roi1,
roi_hierarchy=roi_hierarchy,
object_contour=id_objects,
obj_hierarchy=obj_hierarchy_healthy,
device=device,
debug=args.debug)
device, id_objects, obj_hierarchy_unhealthy = pcv.find_objects(
img=img, mask=fill_image_unhealthy, device=device, debug=args.debug)
device, _, _, kept_mask_unhealthy, _ = pcv.roi_objects(
img=img,
roi_type='partial',
roi_contour=roi1,
roi_hierarchy=roi_hierarchy,
object_contour=id_objects,
obj_hierarchy=obj_hierarchy_unhealthy,
device=device,
debug=args.debug)
# Combine the healthy and unhealthy mask
device, mask = pcv.logical_or(img1=kept_mask_healthy,
img2=kept_mask_unhealthy,
device=device,
debug=args.debug)
# Output a healthy/unhealthy image
classified_img = cv2.merge([
np.zeros(np.shape(mask), dtype=np.uint8), kept_mask_healthy,
kept_mask_unhealthy
])
pcv.print_image(img=classified_img,
filename=os.path.join(
args.outdir,
os.path.basename(args.image)[:-4] + ".classified.png"))
# Output a healthy/unhealthy image overlaid on the original image
overlayed = cv2.addWeighted(src1=np.copy(classified_img),
alpha=0.5,
src2=np.copy(img),
beta=0.5,
gamma=0)
pcv.print_image(img=overlayed,
filename=os.path.join(
args.outdir,
os.path.basename(args.image)[:-4] + ".overlaid.png"))
# Extract hue values from the image
device, h = pcv.rgb2gray_hsv(img=img,
channel="h",
device=device,
debug=args.debug)
# Extract the plant hue values
plant_hues = h[np.where(mask == 255)]
# Initialize hue histogram
hue_hist = {}
for i in range(0, 180):
hue_hist[i] = 0
# Store all hue values
hue_values = []
# Populate histogram
total_px = len(plant_hues)
for hue in plant_hues:
hue_hist[hue] += 1
hue_values.append(hue)
# Parse the filename
genotype, treatment, replicate, timepoint = os.path.basename(
args.image)[:-4].split("_")
replicate = replicate.replace("#", "")
if timepoint[-3:] == "dbi":
timepoint = -1
else:
timepoint = timepoint.replace("dpi", "")
# Output results
for i in range(0, 180):
out.write("\t".join(
map(str, [
genotype, treatment, timepoint, replicate, total_px, i,
hue_hist[i]
])) + "\n")
out.close()
# Calculate basic statistics
healthy_sum = int(np.sum(kept_mask_healthy))
unhealthy_sum = int(np.sum(kept_mask_unhealthy))
healthy_total_ratio = healthy_sum / float(healthy_sum + unhealthy_sum)
unhealthy_total_ratio = unhealthy_sum / float(healthy_sum + unhealthy_sum)
stats = open(args.outfile[:-4] + ".stats.txt", "w")
stats.write("%s, %f, %f, %f, %f" %
(os.path.basename(args.image), healthy_sum, unhealthy_sum,
healthy_total_ratio, unhealthy_total_ratio) + '\n')
stats.close()
# Fit a 3-component Gaussian Mixture Model
gmm = mixture.GaussianMixture(n_components=3,
covariance_type="full",
tol=0.001)
gmm.fit(np.expand_dims(hue_values, 1))
gmm3 = open(args.outfile[:-4] + ".gmm3.txt", "w")
gmm3.write("%s, %f, %f, %f, %f, %f, %f, %f, %f, %f" %
(os.path.basename(args.image), gmm.means_.ravel()[0],
gmm.means_.ravel()[1], gmm.means_.ravel()[2],
np.sqrt(gmm.covariances_.ravel()[0]),
np.sqrt(gmm.covariances_.ravel()[1]),
np.sqrt(gmm.covariances_.ravel()[2]), gmm.weights_.ravel()[0],
gmm.weights_.ravel()[1], gmm.weights_.ravel()[2]) + '\n')
gmm3.close()
# Fit a 2-component Gaussian Mixture Model
gmm = mixture.GaussianMixture(n_components=2,
covariance_type="full",
tol=0.001)
gmm.fit(np.expand_dims(hue_values, 1))
gmm2 = open(args.outfile[:-4] + ".gmm2.txt", "w")
gmm2.write("%s, %f, %f, %f, %f, %f, %f" %
(os.path.basename(args.image), gmm.means_.ravel()[0],
gmm.means_.ravel()[1], np.sqrt(gmm.covariances_.ravel()[0]),
np.sqrt(gmm.covariances_.ravel()[1]), gmm.weights_.ravel()[0],
gmm.weights_.ravel()[1]) + '\n')
gmm2.close()
# Fit a 1-component Gaussian Mixture Model
gmm = mixture.GaussianMixture(n_components=1,
covariance_type="full",
tol=0.001)
gmm.fit(np.expand_dims(hue_values, 1))
gmm1 = open(args.outfile[:-4] + ".gmm1.txt", "w")
gmm1.write(
"%s, %f, %f, %f" %
(os.path.basename(args.image), gmm.means_.ravel()[0],
np.sqrt(gmm.covariances_.ravel()[0]), gmm.weights_.ravel()[0]) + '\n')
gmm1.close()
