def plot_squiggle(args, filename, start_times, mean_signals):
"""
Use rpy2 to create a squiggle plot of the read
"""
r = robjects.r
r.library("ggplot2")
grdevices = importr('grDevices')
# set t_0 as the first measured time for the read.
t_0 = start_times[0]
total_time = start_times[-1] - start_times[0]
# adjust times to be relative to t_0
r_start_times = robjects.FloatVector([t - t_0 for t in start_times])
r_mean_signals = robjects.FloatVector(mean_signals)
# infer the appropriate number of events given the number of facets
num_events = len(r_mean_signals)
events_per_facet = (num_events / args.num_facets) + 1
# dummy variable to control faceting
facet_category = robjects.FloatVector([(i / events_per_facet) + 1 for i in range(len(start_times))])
# make a data frame of the start times and mean signals
d = {'start': r_start_times, 'mean': r_mean_signals, 'cat': facet_category}
df = robjects.DataFrame(d)
gp = ggplot2.ggplot(df)
if not args.theme_bw:
pp = gp + ggplot2.aes_string(x='start', y='mean') \
+ ggplot2.geom_step(size=0.25) \
+ ggplot2.facet_wrap(robjects.Formula('~cat'), ncol=1, scales="free_x") \
+ ggplot2.scale_x_continuous('Time (seconds)') \
+ ggplot2.scale_y_continuous('Mean signal (picoamps)') \
+ ggplot2.ggtitle('Squiggle plot for read: ' + filename + "\nTotal time (sec): " + str(total_time)) \
+ ggplot2.theme(**{'plot.title': ggplot2.element_text(size=11)})
else:
pp = gp + ggplot2.aes_string(x='start', y='mean') \
+ ggplot2.geom_step(size=0.25) \
+ ggplot2.facet_wrap(robjects.Formula('~cat'), ncol=1, scales="free_x") \
+ ggplot2.scale_x_continuous('Time (seconds)') \
+ ggplot2.scale_y_continuous('Mean signal (picoamps)') \
+ ggplot2.ggtitle('Squiggle plot for read: ' + filename + "\nTotal time (sec): " + str(total_time)) \
+ ggplot2.theme(**{'plot.title': ggplot2.element_text(size=11)}) \
+ ggplot2.theme_bw()
if args.saveas is not None:
plot_file = os.path.basename(filename) + "." + args.saveas
if os.path.isfile(plot_file):
raise Exception('Cannot create plot for %s: plot file %s already exists' % (filename, plot_file))
if args.saveas == "pdf":
grdevices.pdf(plot_file, width = 8.5, height = 11)
elif args.saveas == "png":
grdevices.png(plot_file, width = 8.5, height = 11,
units = "in", res = 300)
pp.plot()
grdevices.dev_off()
else:
pp.plot()
# keep the plot open until user hits enter
print('Type enter to exit.')
raw_input()
def plot_collectors_curve(args, start_times, read_lengths):
"""
Use rpy2 to create a collectors curve of the run
"""
r = robjects.r
r.library("ggplot2")
grdevices = importr('grDevices')
# set t_0 as the first measured time for the read.
t_0 = start_times[0]
# adjust times to be relative to t_0
r_start_times = robjects.FloatVector([float(t - t_0) / float(3600) + 0.00000001 \
for t in start_times])
r_read_lengths = robjects.IntVector(read_lengths)
# compute the cumulative based on reads or total base pairs
if args.plot_type == 'reads':
y_label = "Total reads"
cumulative = \
r.cumsum(robjects.IntVector([1] * len(start_times)))
elif args.plot_type == 'basepairs':
y_label = "Total base pairs"
cumulative = r.cumsum(r_read_lengths)
step = args.skip
# make a data frame of the lists
d = {
'start':
robjects.FloatVector(
[r_start_times[n] for n in xrange(0, len(r_start_times), step)]),
'lengths':
robjects.IntVector(
[r_read_lengths[n] for n in xrange(0, len(r_read_lengths), step)]),
'cumul':
robjects.IntVector(
[cumulative[n] for n in xrange(0, len(cumulative), step)])
}
df = robjects.DataFrame(d)
if args.savedf:
robjects.r("write.table")(df, file=args.savedf, sep="\t")
# title
total_reads = len(read_lengths)
total_bp = sum(read_lengths)
plot_title = "Yield: " \
+ str(total_reads) + " reads and " \
+ str(total_bp) + " base pairs."
# plot
gp = ggplot2.ggplot(df)
pp = gp + ggplot2.aes_string(x='start', y='cumul') \
+ ggplot2.geom_step(size=2) \
+ ggplot2.scale_x_continuous('Time (hours)') \
+ ggplot2.scale_y_continuous(y_label) \
+ ggplot2.ggtitle(plot_title)
# extrapolation
if args.extrapolate:
start = robjects.ListVector({'a': 1, 'b': 1})
pp = pp + ggplot2.stat_smooth(fullrange='TRUE', method='nls',
formula='y~a*I((x*3600)^b)',
se='FALSE', start=start) \
+ ggplot2.xlim(0, float(args.extrapolate))
if args.theme_bw:
pp = pp + ggplot2.theme_bw()
if args.saveas is not None:
plot_file = args.saveas
if plot_file.endswith(".pdf"):
grdevices.pdf(plot_file, width=8.5, height=8.5)
elif plot_file.endswith(".png"):
grdevices.png(plot_file,
width=8.5,
height=8.5,
units="in",
res=300)
else:
logger.error("Unrecognized extension for %s!" % (plot_file))
sys.exit()
pp.plot()
grdevices.dev_off()
else:
pp.plot()
# keep the plot open until user hits enter
print('Type enter to exit.')
raw_input()
def plot_collectors_curve(args, start_times, read_lengths):
"""
Use rpy2 to create a collectors curve of the run
"""
r = robjects.r
r.library("ggplot2")
grdevices = importr('grDevices')
# set t_0 as the first measured time for the read.
t_0 = start_times[0]
# adjust times to be relative to t_0
r_start_times = robjects.FloatVector([float(t - t_0) / float(3600) + 0.00000001 \
for t in start_times])
r_read_lengths = robjects.IntVector(read_lengths)
# compute the cumulative based on reads or total base pairs
if args.plot_type == 'reads':
y_label = "Total reads"
cumulative = \
r.cumsum(robjects.IntVector([1] * len(start_times)))
elif args.plot_type == 'basepairs':
y_label = "Total base pairs"
cumulative = r.cumsum(r_read_lengths)
# make a data frame of the lists
d = {'start': r_start_times,
'lengths': r_read_lengths,
'cumul': cumulative}
df = robjects.DataFrame(d)
if args.savedf:
robjects.r("write.table")(df, file=args.savedf, sep="\t")
# title
total_reads = len(read_lengths)
total_bp = sum(read_lengths)
plot_title = "Yield: " \
+ str(total_reads) + " reads and " \
+ str(total_bp) + " base pairs."
# plot
gp = ggplot2.ggplot(df)
pp = gp + ggplot2.aes_string(x='start', y='cumul') \
+ ggplot2.geom_step(size=2) \
+ ggplot2.scale_x_continuous('Time (hours)') \
+ ggplot2.scale_y_continuous(y_label) \
+ ggplot2.ggtitle(plot_title)
# extrapolation
if args.extrapolate:
start = robjects.ListVector({'a': 1, 'b': 1})
pp = pp + ggplot2.stat_smooth(fullrange='TRUE', method='nls',
formula='y~a*I((x*3600)^b)',
se='FALSE', start=start) \
+ ggplot2.xlim(0, float(args.extrapolate))
if args.theme_bw:
pp = pp + ggplot2.theme_bw()
if args.saveas is not None:
plot_file = args.saveas
if plot_file.endswith(".pdf"):
grdevices.pdf(plot_file, width = 8.5, height = 8.5)
elif plot_file.endswith(".png"):
grdevices.png(plot_file, width = 8.5, height = 8.5,
units = "in", res = 300)
else:
logger.error("Unrecognized extension for %s!" % (plot_file))
sys.exit()
pp.plot()
grdevices.dev_off()
else:
pp.plot()
# keep the plot open until user hits enter
print('Type enter to exit.')
raw_input()
def plot_squiggle(args, filename, start_times, mean_signals):
"""
Use rpy2 to create a squiggle plot of the read
"""
r = robjects.r
r.library("ggplot2")
grdevices = importr('grDevices')
# set t_0 as the first measured time for the read.
t_0 = start_times[0]
total_time = start_times[-1] - start_times[0]
# adjust times to be relative to t_0
r_start_times = robjects.FloatVector([t - t_0 for t in start_times])
r_mean_signals = robjects.FloatVector(mean_signals)
# infer the appropriate number of events given the number of facets
num_events = len(r_mean_signals)
events_per_facet = (num_events / args.num_facets) + 1
# dummy variable to control faceting
facet_category = robjects.FloatVector([(i / events_per_facet) + 1
for i in range(len(start_times))])
# make a data frame of the start times and mean signals
d = {'start': r_start_times, 'mean': r_mean_signals, 'cat': facet_category}
df = robjects.DataFrame(d)
gp = ggplot2.ggplot(df)
if not args.theme_bw:
pp = gp + ggplot2.aes_string(x='start', y='mean') \
+ ggplot2.geom_step(size=0.25) \
+ ggplot2.facet_wrap(robjects.Formula('~cat'), ncol=1, scales="free_x") \
+ ggplot2.scale_x_continuous('Time (seconds)') \
+ ggplot2.scale_y_continuous('Mean signal (picoamps)') \
+ ggplot2.ggtitle('Squiggle plot for read: ' + filename + "\nTotal time (sec): " + str(total_time)) \
+ ggplot2.theme(**{'plot.title': ggplot2.element_text(size=11)})
else:
pp = gp + ggplot2.aes_string(x='start', y='mean') \
+ ggplot2.geom_step(size=0.25) \
+ ggplot2.facet_wrap(robjects.Formula('~cat'), ncol=1, scales="free_x") \
+ ggplot2.scale_x_continuous('Time (seconds)') \
+ ggplot2.scale_y_continuous('Mean signal (picoamps)') \
+ ggplot2.ggtitle('Squiggle plot for read: ' + filename + "\nTotal time (sec): " + str(total_time)) \
+ ggplot2.theme(**{'plot.title': ggplot2.element_text(size=11)}) \
+ ggplot2.theme_bw()
if args.saveas is not None:
plot_file = os.path.basename(filename) + "." + args.saveas
if os.path.isfile(plot_file):
raise Exception(
'Cannot create plot for %s: plot file %s already exists' %
(filename, plot_file))
if args.saveas == "pdf":
grdevices.pdf(plot_file, width=8.5, height=11)
elif args.saveas == "png":
grdevices.png(plot_file, width=8.5, height=11, units="in", res=300)
pp.plot()
grdevices.dev_off()
else:
pp.plot()
# keep the plot open until user hits enter
print('Type enter to exit.')
raw_input()
