def update_z_edge(frame):
'''Frame updater for FuncAnimation.
'''
im_xy.set_data(imgs_rna[chan][:,:,frame])
colors = []
for spot in spots_masked:
alpha = su.gauss_1d(frame, 1., spot[2], spot[3])
colors.append([1., 0., 0., alpha])
spots_xy.set_edgecolor(colors)
return im_xy, spots_xy # this return structure is a requirement for the FuncAnimation() callable
def update_z_compartments(frame):
'''Frame updater for FuncAnimation.
'''
im_xy.set_data(imgs_rna[chan][:,:,frame])
colors_nuc = []
colors_cyt = []
colors_peri = []
for spot in spots_by_region['nuc']:
alpha = su.gauss_1d(frame, 1., spot[2], spot[3])
colors_nuc.append([0.3, 0.3, 1., alpha])
for spot in spots_by_region['cyt']:
alpha = su.gauss_1d(frame, 1., spot[2], spot[3])
colors_cyt.append([1., 0.3, 0.3, alpha])
for spot in spots_by_region['peri']:
alpha = su.gauss_1d(frame, 1., spot[2], spot[3])
colors_peri.append([0.3, 1., 0.3, alpha])
spots_nuc_xy.set_facecolor(colors_nuc)
spots_cyt_xy.set_facecolor(colors_cyt)
spots_peri_xy.set_facecolor(colors_peri)
return im_xy, spots_nuc_xy, spots_cyt_xy, spots_peri_xy # this return structure is a requirement for the FuncAnimation() callable
def update_z_grassfire(frame):
'''Frame updater for FuncAnimation.
'''
dapi_xy.set_data(img_dapi[:,:,frame])
im_xy.set_data(imgs_rna[chan][:,:,frame])
colors = spots_xy.get_facecolor()
for i, spot in enumerate(spots_xyz):
alpha = su.gauss_1d(frame, 1., spot[2]/dims['Z'], 1.)
colors[i,3] = alpha
spots_xy.set_facecolor(colors)
return dapi_xy, im_xy, spots_xy # this return structure is a requirement for the FuncAnimation() callable