def run_spades(configs_dir, execution_home, cfg, dataset_data,
ext_python_modules_home, log):
if not isinstance(cfg.iterative_K, list):
cfg.iterative_K = [cfg.iterative_K]
cfg.iterative_K = sorted(cfg.iterative_K)
used_K = []
# checking and removing conflicting K-mer directories
if options_storage.restart_from and (options_storage.restart_k_mers !=
options_storage.original_k_mers):
processed_K = []
for k in range(options_storage.MIN_K, options_storage.MAX_K, 2):
cur_K_dir = os.path.join(cfg.output_dir, "K%d" % k)
if os.path.isdir(cur_K_dir) and os.path.isfile(
os.path.join(cur_K_dir, "final_contigs.fasta")):
processed_K.append(k)
if processed_K:
RL = get_read_length(cfg.output_dir, processed_K[0],
ext_python_modules_home, log)
needed_K = update_k_mers_in_special_cases(cfg.iterative_K,
RL,
log,
silent=True)
needed_K = [k for k in needed_K if k < RL]
original_K = reveal_original_k_mers(RL)
k_to_delete = []
for id, k in enumerate(needed_K):
if len(processed_K) == id:
if processed_K[-1] == original_K[
-1]: # the last K in the original run was processed in "last_one" mode
k_to_delete = [original_K[-1]]
break
if processed_K[id] != k:
k_to_delete = processed_K[id:]
break
if not k_to_delete and (len(processed_K) > len(needed_K)):
k_to_delete = processed_K[len(needed_K) - 1:]
if k_to_delete:
log.info(
"Restart mode: removing previously processed directories for K=%s "
"to avoid conflicts with K specified with --restart-from" %
(str(k_to_delete)))
for k in k_to_delete:
shutil.rmtree(os.path.join(cfg.output_dir, "K%d" % k))
bin_reads_dir = os.path.join(cfg.output_dir, ".bin_reads")
if os.path.isdir(bin_reads_dir) and not options_storage.continue_mode:
shutil.rmtree(bin_reads_dir)
cfg.tmp_dir = support.get_tmp_dir(prefix="spades_")
finished_on_stop_after = False
K = cfg.iterative_K[0]
if len(cfg.iterative_K) == 1:
run_iteration(configs_dir, execution_home, cfg, log, K, None, True)
used_K.append(K)
else:
run_iteration(configs_dir, execution_home, cfg, log, K, None, False)
used_K.append(K)
if options_storage.stop_after == "k%d" % K:
finished_on_stop_after = True
else:
prev_K = K
RL = get_read_length(cfg.output_dir, K, ext_python_modules_home,
log)
cfg.iterative_K = update_k_mers_in_special_cases(
cfg.iterative_K, RL, log)
if len(cfg.iterative_K) < 2 or cfg.iterative_K[1] + 1 > RL:
if cfg.rr_enable:
if len(cfg.iterative_K) < 2:
log.info(
"== Rerunning for the first value of K (%d) with Repeat Resolving"
% cfg.iterative_K[0])
else:
support.warning(
"Second value of iterative K (%d) exceeded estimated read length (%d). "
"Rerunning for the first value of K (%d) with Repeat Resolving"
% (cfg.iterative_K[1], RL, cfg.iterative_K[0]),
log)
run_iteration(configs_dir, execution_home, cfg, log,
cfg.iterative_K[0], None, True)
used_K.append(cfg.iterative_K[0])
K = cfg.iterative_K[0]
else:
rest_of_iterative_K = cfg.iterative_K
rest_of_iterative_K.pop(0)
count = 0
for K in rest_of_iterative_K:
count += 1
last_one = count == len(cfg.iterative_K) or (
rest_of_iterative_K[count] + 1 > RL)
run_iteration(configs_dir, execution_home, cfg, log, K,
prev_K, last_one)
used_K.append(K)
prev_K = K
if last_one:
break
if options_storage.stop_after == "k%d" % K:
finished_on_stop_after = True
break
if count < len(cfg.iterative_K) and not finished_on_stop_after:
support.warning(
"Iterations stopped. Value of K (%d) exceeded estimated read length (%d)"
% (cfg.iterative_K[count], RL), log)
if options_storage.stop_after and options_storage.stop_after.startswith(
'k'):
support.finish_here(log)
latest = os.path.join(cfg.output_dir, "K%d" % K)
if cfg.correct_scaffolds and not options_storage.run_completed:
if options_storage.continue_mode and os.path.isfile(
os.path.join(cfg.output_dir, "SCC", "corrected_scaffolds.fasta"
)) and not options_storage.restart_from == "scc":
log.info("\n===== Skipping %s (already processed). \n" %
"scaffold correction")
else:
if options_storage.continue_mode:
support.continue_from_here(log)
run_scaffold_correction(configs_dir, execution_home, cfg, log,
latest, 21)
latest = os.path.join(os.path.join(cfg.output_dir, "SCC"), "K21")
if options_storage.stop_after == 'scc':
support.finish_here(log)
if cfg.correct_scaffolds:
correct_scaffolds_fpath = os.path.join(latest,
"corrected_scaffolds.fasta")
if os.path.isfile(correct_scaffolds_fpath):
shutil.copyfile(correct_scaffolds_fpath, cfg.result_scaffolds)
elif not finished_on_stop_after: # interupted by --stop-after, so final K is not processed!
if os.path.isfile(os.path.join(latest, "before_rr.fasta")):
result_before_rr_contigs = os.path.join(
os.path.dirname(cfg.result_contigs), "before_rr.fasta")
if not os.path.isfile(result_before_rr_contigs
) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "before_rr.fasta"),
result_before_rr_contigs)
if options_storage.rna:
if os.path.isfile(os.path.join(latest, "transcripts.fasta")):
if not os.path.isfile(cfg.result_transcripts
) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "transcripts.fasta"),
cfg.result_transcripts)
if os.path.isfile(os.path.join(latest, "transcripts.paths")):
if not os.path.isfile(cfg.result_transcripts_paths
) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "transcripts.paths"),
cfg.result_transcripts_paths)
for filtering_type in options_storage.filtering_types:
prefix = filtering_type + "_filtered_"
result_filtered_transcripts = os.path.join(
cfg.output_dir, prefix + options_storage.transcripts_name)
latest_filtered_transcripts = os.path.join(
latest, prefix + "final_paths.fasta")
if os.path.isfile(latest_filtered_transcripts):
if not os.path.isfile(
result_filtered_transcripts
) or not options_storage.continue_mode:
shutil.copyfile(latest_filtered_transcripts,
result_filtered_transcripts)
else:
if os.path.isfile(os.path.join(latest, "final_contigs.fasta")):
if not os.path.isfile(cfg.result_contigs
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "final_contigs.fasta"),
cfg.result_contigs)
if os.path.isfile(os.path.join(latest, "first_pe_contigs.fasta")):
result_first_pe_contigs = os.path.join(
os.path.dirname(cfg.result_contigs),
"first_pe_contigs.fasta")
if not os.path.isfile(result_first_pe_contigs
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "first_pe_contigs.fasta"),
result_first_pe_contigs)
if cfg.rr_enable:
if os.path.isfile(os.path.join(latest, "scaffolds.fasta")):
if not os.path.isfile(
cfg.result_scaffolds
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "scaffolds.fasta"),
cfg.result_scaffolds)
if os.path.isfile(os.path.join(latest, "scaffolds.paths")):
if not os.path.isfile(
cfg.result_scaffolds_paths
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "scaffolds.paths"),
cfg.result_scaffolds_paths)
if os.path.isfile(
os.path.join(latest, "assembly_graph_with_scaffolds.gfa")):
if not os.path.isfile(cfg.result_graph_gfa
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest,
"assembly_graph_with_scaffolds.gfa"),
cfg.result_graph_gfa)
if os.path.isfile(os.path.join(latest, "assembly_graph.fastg")):
if not os.path.isfile(
cfg.result_graph) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "assembly_graph.fastg"),
cfg.result_graph)
if os.path.isfile(os.path.join(latest, "final_contigs.paths")):
if not os.path.isfile(cfg.result_contigs_paths
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "final_contigs.paths"),
cfg.result_contigs_paths)
if cfg.developer_mode:
# saves
saves_link = os.path.join(os.path.dirname(cfg.result_contigs), "saves")
if os.path.lexists(
saves_link
): # exists returns False for broken links! lexists return True
os.remove(saves_link)
os.symlink(os.path.join(latest, "saves"), saves_link)
if os.path.isdir(bin_reads_dir):
shutil.rmtree(bin_reads_dir)
if os.path.isdir(cfg.tmp_dir):
shutil.rmtree(cfg.tmp_dir)
return used_K
def run_mulksg(configs_dir, execution_home, cfg, dataset_data,
ext_python_modules_home, log, comm, rank, size):
#Running this on multiple nodes, need to differentiate the actual work here
if not isinstance(cfg.iterative_K, list):
cfg.iterative_K = [cfg.iterative_K]
cfg.iterative_K = sorted(cfg.iterative_K)
while (len(cfg.iterative_K) - 1) % size != 0:
bgap_idx = 0
bgap = 0
for i in range(0, len(cfg.iterative_K) - 1):
gap = cfg.iterative_K[i + 1] - cfg.iterative_K[i]
if gap > bgap:
bgap = gap
bgap_idx = i
if bgap == 0:
break
ul = cfg.iterative_K[i + 1]
ll = cfg.iterative_K[i]
new_k = (int)(ul - ll) / 2
new_k = new_k / 2 * 2 + 1
cfg.iterative_K.append(new_k)
cfg.iterative_K = sorted(cfg.iterative_K)
#add to cfg.iterative_K
#add a number between the biggest gaps in the current cfg.iterative_K
if rank == 0: #Setup all the configs, we will put them in a work_list
# checking and removing conflicting K-mer directories
if options_storage.restart_from and (options_storage.restart_k_mers !=
options_storage.original_k_mers):
processed_K = []
for k in range(options_storage.MIN_K, options_storage.MAX_K, 2):
cur_K_dir = os.path.join(cfg.output_dir, "K%d" % k)
if os.path.isdir(cur_K_dir) and os.path.isfile(
os.path.join(cur_K_dir, "final_contigs.fasta")):
processed_K.append(k)
if processed_K:
RL = get_read_length(cfg.output_dir, processed_K[0],
ext_python_modules_home, log)
needed_K = update_k_mers_in_special_cases(cfg.iterative_K,
RL,
log,
silent=True)
needed_K = [k for k in needed_K if k < RL]
original_K = reveal_original_k_mers(RL)
k_to_delete = []
for id, k in enumerate(needed_K):
if len(processed_K) == id:
if processed_K[-1] == original_K[
-1]: # the last K in the original run was processed in "last_one" mode
k_to_delete = [original_K[-1]]
break
if processed_K[id] != k:
k_to_delete = processed_K[id:]
break
if not k_to_delete and (len(processed_K) > len(needed_K)):
k_to_delete = processed_K[len(needed_K) - 1:]
if k_to_delete:
log.info(
"Restart mode: removing previously processed directories for K=%s "
"to avoid conflicts with K specified with --restart-from"
% (str(k_to_delete)))
for k in k_to_delete:
shutil.rmtree(os.path.join(cfg.output_dir, "K%d" % k))
bin_reads_dir = os.path.join(cfg.output_dir, ".bin_reads")
if os.path.isdir(bin_reads_dir):
shutil.rmtree(bin_reads_dir)
cfg.tmp_dir = support.get_tmp_dir(prefix="mulksg_")
first_kValues = cfg.iterative_K
last_kValue = []
last_kValue.append(first_kValues[-1])
del first_kValues[-1]
#process all of the files as last_one
first_commands, contig_files = create_mulksg_configs(
configs_dir, execution_home, cfg, log, first_kValues, None, True,
ext_python_modules_home)
work_list = first_commands
if size > 1 and len(cfg.iterative_K) > 1:
num_running = 0
for command in work_list:
if num_running == (size - 1):
support.sys_call(command, log)
num_running = 0
else:
node_rank = comm.recv() #source=ANY_SOURCE
comm.send(command, node_rank)
num_running += 1
for i in range(1, size):
node_rank = comm.recv() #source=ANY_SOURCE
comm.send("Done", node_rank)
print "Rank", node_rank, "Finished"
else:
if len(cfg.iterative_K) > 1:
pool = Pool(len(first_commands))
results = pool.map(
support.sys_call,
first_commands) #(command for command in first_commands))
#Now we have generated all the contig files, concatenate into one file
extra_contig_filename = None
try:
extra_contig_filename = cfg.output_dir + "/extracontigs.fa" #"/K" + last_kValue[0]
with open(extra_contig_filename, 'w') as contig_file:
for fname in contig_files:
try:
with open(fname) as infile:
contig_file.write(infile.read())
except:
continue
except:
log.info("Could not create extra contig file!!!!")
extra_contig_filename = None
last_command, contig_files = create_mulksg_configs(
configs_dir, execution_home, cfg, log, last_kValue,
extra_contig_filename, True, ext_python_modules_home)
last_command = last_command[0]
support.sys_call(last_command, log)
K = last_kValue[0]
latest = os.path.join(cfg.output_dir, "K%d" % K)
finished_on_stop_after = False
if cfg.correct_scaffolds and not options_storage.run_completed:
if options_storage.continue_mode and os.path.isfile(
os.path.join(cfg.output_dir, "SCC",
"corrected_scaffolds.fasta")
) and not options_storage.restart_from == "scc":
log.info("\n===== Skipping %s (already processed). \n" %
"scaffold correction")
else:
if options_storage.continue_mode:
support.continue_from_here(log)
run_scaffold_correction(configs_dir, execution_home, cfg, log,
latest, 21)
latest = os.path.join(os.path.join(cfg.output_dir, "SCC"), "K21")
if options_storage.stop_after == 'scc':
support.finish_here(log)
if cfg.correct_scaffolds:
correct_scaffolds_fpath = os.path.join(
latest, "corrected_scaffolds.fasta")
if os.path.isfile(correct_scaffolds_fpath):
shutil.copyfile(correct_scaffolds_fpath, cfg.result_scaffolds)
elif not finished_on_stop_after: # interupted by --stop-after, so final K is not processed!
if os.path.isfile(os.path.join(latest, "before_rr.fasta")):
result_before_rr_contigs = os.path.join(
os.path.dirname(cfg.result_contigs), "before_rr.fasta")
if not os.path.isfile(result_before_rr_contigs
) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "before_rr.fasta"),
result_before_rr_contigs)
if options_storage.rna:
if os.path.isfile(os.path.join(latest, "transcripts.fasta")):
if not os.path.isfile(
cfg.result_transcripts
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "transcripts.fasta"),
cfg.result_transcripts)
if os.path.isfile(os.path.join(latest, "transcripts.paths")):
if not os.path.isfile(
cfg.result_transcripts_paths
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "transcripts.paths"),
cfg.result_transcripts_paths)
for filtering_type in options_storage.filtering_types:
prefix = filtering_type + "_filtered_"
result_filtered_transcripts = os.path.join(
cfg.output_dir,
prefix + options_storage.transcripts_name)
latest_filtered_transcripts = os.path.join(
latest, prefix + "final_paths.fasta")
if os.path.isfile(latest_filtered_transcripts):
if not os.path.isfile(
result_filtered_transcripts
) or not options_storage.continue_mode:
shutil.copyfile(latest_filtered_transcripts,
result_filtered_transcripts)
else:
if os.path.isfile(os.path.join(latest, "final_contigs.fasta")):
if not os.path.isfile(
cfg.result_contigs
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "final_contigs.fasta"),
cfg.result_contigs)
if os.path.isfile(
os.path.join(latest, "first_pe_contigs.fasta")):
result_first_pe_contigs = os.path.join(
os.path.dirname(cfg.result_contigs),
"first_pe_contigs.fasta")
if not os.path.isfile(
result_first_pe_contigs
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "first_pe_contigs.fasta"),
result_first_pe_contigs)
if cfg.rr_enable:
if os.path.isfile(os.path.join(latest, "scaffolds.fasta")):
if not os.path.isfile(
cfg.result_scaffolds
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "scaffolds.fasta"),
cfg.result_scaffolds)
if os.path.isfile(os.path.join(latest, "scaffolds.paths")):
if not os.path.isfile(
cfg.result_scaffolds_paths
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "scaffolds.paths"),
cfg.result_scaffolds_paths)
if os.path.isfile(
os.path.join(latest,
"assembly_graph_with_scaffolds.gfa")):
if not os.path.isfile(
cfg.result_graph_gfa
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest,
"assembly_graph_with_scaffolds.gfa"),
cfg.result_graph_gfa)
if os.path.isfile(os.path.join(latest,
"assembly_graph.fastg")):
if not os.path.isfile(
cfg.result_graph
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "assembly_graph.fastg"),
cfg.result_graph)
if os.path.isfile(os.path.join(latest, "final_contigs.paths")):
if not os.path.isfile(
cfg.result_contigs_paths
) or not options_storage.continue_mode:
shutil.copyfile(
os.path.join(latest, "final_contigs.paths"),
cfg.result_contigs_paths)
if os.path.isdir(bin_reads_dir):
shutil.rmtree(bin_reads_dir)
if os.path.isdir(cfg.tmp_dir):
shutil.rmtree(cfg.tmp_dir)
return first_kValues + last_kValue
elif rank != 0 and len(cfg.iterative_K) > 1:
comm.send(rank, 0)
command = comm.recv(source=0)
while command != "Done":
# print rank, command
support.sys_call(command, log)
comm.send(rank, 0)
command = comm.recv(source=0)
# comm.send(rank, 0) #tell the master I am done and exiting
return None
def main(args):
os.environ["LC_ALL"] = "C"
if len(args) == 1:
show_usage(0)
log = logging.getLogger('spades')
log.setLevel(logging.DEBUG)
console = logging.StreamHandler(sys.stdout)
console.setFormatter(logging.Formatter('%(message)s'))
console.setLevel(logging.DEBUG)
log.addHandler(console)
support.check_binaries(bin_home, log)
# parse options and safe all parameters to cfg
options = args
cfg, dataset_data = fill_cfg(options, log)
if options_storage.continue_mode:
cmd_line, options = get_options_from_params(os.path.join(options_storage.output_dir, "params.txt"), args[0])
if not options:
support.error("failed to parse command line of the previous run! Please restart from the beginning or specify another output directory.")
cfg, dataset_data = fill_cfg(options, log, secondary_filling=True)
if options_storage.restart_from:
check_cfg_for_partial_run(cfg, type='restart-from')
options_storage.continue_mode = True
if options_storage.stop_after:
check_cfg_for_partial_run(cfg, type='stop-after')
log_filename = os.path.join(cfg["common"].output_dir, "spades.log")
if options_storage.continue_mode:
log_handler = logging.FileHandler(log_filename, mode='a')
else:
log_handler = logging.FileHandler(log_filename, mode='w')
log.addHandler(log_handler)
if options_storage.continue_mode:
log.info("\n======= SPAdes pipeline continued. Log can be found here: " + log_filename + "\n")
log.info("Restored from " + cmd_line)
if options_storage.restart_from:
updated_params = ""
skip_next = False
for v in args[1:]:
if v == '-o' or v == '--restart-from':
skip_next = True
continue
if skip_next or v.startswith('--restart-from='): # you can specify '--restart-from=k33' but not '-o=out_dir'
skip_next = False
continue
updated_params += " " + v
updated_params = updated_params.strip()
log.info("with updated parameters: " + updated_params)
cmd_line += " " + updated_params
log.info("")
params_filename = os.path.join(cfg["common"].output_dir, "params.txt")
params_handler = logging.FileHandler(params_filename, mode='w')
log.addHandler(params_handler)
if options_storage.continue_mode:
log.info(cmd_line)
else:
command = "Command line: "
for v in args:
# substituting relative paths with absolute ones (read paths, output dir path, etc)
v, prefix = support.get_option_prefix(v)
if v in options_storage.dict_of_rel2abs.keys():
v = options_storage.dict_of_rel2abs[v]
if prefix:
command += prefix + ":"
command += v + " "
log.info(command)
# special case
# if "mismatch_corrector" in cfg and not support.get_lib_ids_by_type(dataset_data, 'paired-end'):
# support.warning('cannot perform mismatch correction without at least one paired-end library! Skipping this step.', log)
# del cfg["mismatch_corrector"]
print_used_values(cfg, log)
log.removeHandler(params_handler)
support.check_single_reads_in_options(options, log)
if not options_storage.continue_mode:
log.info("\n======= SPAdes pipeline started. Log can be found here: " + log_filename + "\n")
# splitting interlaced reads and processing Ns in additional contigs if needed
if support.dataset_has_interlaced_reads(dataset_data) or support.dataset_has_additional_contigs(dataset_data)\
or support.dataset_has_nxmate_reads(dataset_data):
dir_for_split_reads = os.path.join(options_storage.output_dir, 'split_input')
if support.dataset_has_interlaced_reads(dataset_data) or support.dataset_has_nxmate_reads(dataset_data):
if not os.path.isdir(dir_for_split_reads):
os.makedirs(dir_for_split_reads)
if support.dataset_has_interlaced_reads(dataset_data):
dataset_data = support.split_interlaced_reads(dataset_data, dir_for_split_reads, log)
if support.dataset_has_nxmate_reads(dataset_data):
dataset_data = support.process_nxmate_reads(dataset_data, dir_for_split_reads, log)
if support.dataset_has_additional_contigs(dataset_data):
dataset_data = support.process_Ns_in_additional_contigs(dataset_data, dir_for_split_reads, log)
options_storage.dataset_yaml_filename = os.path.join(options_storage.output_dir, "input_dataset.yaml")
pyyaml.dump(dataset_data, open(options_storage.dataset_yaml_filename, 'w'))
cfg["dataset"].yaml_filename = options_storage.dataset_yaml_filename
try:
# copying configs before all computations (to prevent its changing at run time)
tmp_configs_dir = os.path.join(cfg["common"].output_dir, "configs")
if os.path.isdir(tmp_configs_dir) and not options_storage.continue_mode:
shutil.rmtree(tmp_configs_dir)
if not os.path.isdir(tmp_configs_dir):
if options_storage.configs_dir:
dir_util.copy_tree(options_storage.configs_dir, tmp_configs_dir, preserve_times=False)
else:
dir_util.copy_tree(os.path.join(spades_home, "configs"), tmp_configs_dir, preserve_times=False)
corrected_dataset_yaml_filename = ''
if "error_correction" in cfg:
STAGE_NAME = "Read error correction"
bh_cfg = merge_configs(cfg["error_correction"], cfg["common"])
corrected_dataset_yaml_filename = os.path.join(bh_cfg.output_dir, "corrected.yaml")
if os.path.isfile(corrected_dataset_yaml_filename) and options_storage.continue_mode \
and not options_storage.restart_from == "ec":
log.info("\n===== Skipping %s (already processed). \n" % STAGE_NAME)
else:
support.continue_from_here(log)
if "HEAPCHECK" in os.environ:
del os.environ["HEAPCHECK"]
if "heap_check" in bh_cfg.__dict__:
os.environ["HEAPCHECK"] = bh_cfg.heap_check
if os.path.exists(bh_cfg.output_dir):
shutil.rmtree(bh_cfg.output_dir)
os.makedirs(bh_cfg.output_dir)
if support.get_lib_ids_by_type(dataset_data, options_storage.LONG_READS_TYPES):
not_used_dataset_data = support.get_libs_by_type(dataset_data, options_storage.LONG_READS_TYPES)
to_correct_dataset_data = support.rm_libs_by_type(dataset_data, options_storage.LONG_READS_TYPES)
to_correct_dataset_yaml_filename = os.path.join(bh_cfg.output_dir, "to_correct.yaml")
pyyaml.dump(to_correct_dataset_data, open(to_correct_dataset_yaml_filename, 'w'))
bh_cfg.__dict__["dataset_yaml_filename"] = to_correct_dataset_yaml_filename
else:
not_used_dataset_data = None
bh_cfg.__dict__["dataset_yaml_filename"] = cfg["dataset"].yaml_filename
log.info("\n===== %s started. \n" % STAGE_NAME)
hammer_logic.run_hammer(corrected_dataset_yaml_filename, tmp_configs_dir, bin_home, bh_cfg, not_used_dataset_data,
ext_python_modules_home, log)
log.info("\n===== %s finished. \n" % STAGE_NAME)
if options_storage.stop_after == 'ec':
support.finish_here(log)
result_contigs_filename = os.path.join(cfg["common"].output_dir, "contigs.fasta")
result_scaffolds_filename = os.path.join(cfg["common"].output_dir, "scaffolds.fasta")
result_assembly_graph_filename = os.path.join(cfg["common"].output_dir, "assembly_graph.fastg")
truseq_long_reads_file_base = os.path.join(cfg["common"].output_dir, "truseq_long_reads")
truseq_long_reads_file = truseq_long_reads_file_base + ".fasta"
misc_dir = os.path.join(cfg["common"].output_dir, "misc")
### if mismatch correction is enabled then result contigs are copied to misc directory
assembled_contigs_filename = os.path.join(misc_dir, "assembled_contigs.fasta")
assembled_scaffolds_filename = os.path.join(misc_dir, "assembled_scaffolds.fasta")
if "assembly" in cfg and not options_storage.run_completed:
STAGE_NAME = "Assembling"
spades_cfg = merge_configs(cfg["assembly"], cfg["common"])
spades_cfg.__dict__["result_contigs"] = result_contigs_filename
spades_cfg.__dict__["result_scaffolds"] = result_scaffolds_filename
spades_cfg.__dict__["result_graph"] = result_assembly_graph_filename
if options_storage.continue_mode and (os.path.isfile(spades_cfg.result_contigs)
or ("mismatch_corrector" in cfg and
os.path.isfile(assembled_contigs_filename)))\
and not options_storage.restart_from == 'as' \
and not options_storage.restart_from == 'scc' \
and not (options_storage.restart_from and options_storage.restart_from.startswith('k')):
log.info("\n===== Skipping %s (already processed). \n" % STAGE_NAME)
# calculating latest_dir for the next stages
latest_dir = support.get_latest_dir(os.path.join(spades_cfg.output_dir, "K*"))
if not latest_dir:
support.error("failed to continue the previous run! Please restart from previous stages or from the beginning.", log)
else:
old_result_files = [result_contigs_filename, result_scaffolds_filename,
assembled_contigs_filename, assembled_scaffolds_filename]
for old_result_file in old_result_files:
if os.path.isfile(old_result_file):
os.remove(old_result_file)
if options_storage.restart_from == 'as':
support.continue_from_here(log)
if os.path.isfile(corrected_dataset_yaml_filename):
dataset_data = pyyaml.load(open(corrected_dataset_yaml_filename, 'r'))
dataset_data = support.relative2abs_paths(dataset_data, os.path.dirname(corrected_dataset_yaml_filename))
if spades_cfg.disable_rr:
spades_cfg.__dict__["rr_enable"] = False
else:
spades_cfg.__dict__["rr_enable"] = True
if "HEAPCHECK" in os.environ:
del os.environ["HEAPCHECK"]
if "heap_check" in spades_cfg.__dict__:
os.environ["HEAPCHECK"] = spades_cfg.heap_check
log.info("\n===== %s started.\n" % STAGE_NAME)
# creating dataset
dataset_filename = os.path.join(spades_cfg.output_dir, "dataset.info")
if not os.path.isfile(dataset_filename) or not options_storage.continue_mode:
dataset_file = open(dataset_filename, 'w')
import process_cfg
dataset_file.write("single_cell" + '\t' + process_cfg.bool_to_str(cfg["dataset"].single_cell) + '\n')
dataset_file.write("meta" + '\t' + process_cfg.bool_to_str(cfg["dataset"].meta) + '\n')
dataset_file.write("moleculo" + '\t' + process_cfg.bool_to_str(cfg["dataset"].truseq) + '\n')
if os.path.isfile(corrected_dataset_yaml_filename):
dataset_file.write("reads" + '\t' + process_cfg.process_spaces(corrected_dataset_yaml_filename) + '\n')
else:
dataset_file.write("reads" + '\t' + process_cfg.process_spaces(cfg["dataset"].yaml_filename) + '\n')
if spades_cfg.developer_mode and "reference" in cfg["dataset"].__dict__:
dataset_file.write("reference_genome" + '\t')
dataset_file.write(process_cfg.process_spaces(cfg["dataset"].reference) + '\n')
dataset_file.close()
spades_cfg.__dict__["dataset"] = dataset_filename
latest_dir = spades_logic.run_spades(tmp_configs_dir, bin_home, spades_cfg, dataset_data, ext_python_modules_home, log)
if os.path.isdir(misc_dir) and not options_storage.continue_mode:
shutil.rmtree(misc_dir)
if not os.path.isdir(misc_dir):
os.makedirs(misc_dir)
if options_storage.continue_mode and options_storage.restart_from and options_storage.restart_from.startswith('k'):
k_str = options_storage.restart_from[1:]
if k_str.find(":") != -1:
k_str = k_str[:k_str.find(":")]
support.error("failed to continue from K=%s because this K was not processed in the original run!" % k_str, log)
log.info("\n===== %s finished. \n" % STAGE_NAME)
if not options_storage.run_completed:
if options_storage.stop_after == 'as' or options_storage.stop_after == 'scc' or (options_storage.stop_after and options_storage.stop_after.startswith('k')):
support.finish_here(log)
#postprocessing
if cfg["run_truseq_postprocessing"] and not options_storage.run_completed:
if options_storage.continue_mode and os.path.isfile(truseq_long_reads_file_base + ".fastq") and not options_storage.restart_from == 'tpp':
log.info("\n===== Skipping %s (already processed). \n" % "TruSeq postprocessing")
else:
support.continue_from_here(log)
if os.path.isfile(result_scaffolds_filename):
shutil.move(result_scaffolds_filename, assembled_scaffolds_filename)
reads_library = dataset_data[0]
alignment_bin = os.path.join(bin_home, "bwa-spades")
alignment_dir = os.path.join(cfg["common"].output_dir, "alignment")
sam_files = alignment.align_bwa(alignment_bin, assembled_scaffolds_filename, dataset_data, alignment_dir, log, options_storage.threads)
moleculo_postprocessing.moleculo_postprocessing(assembled_scaffolds_filename, truseq_long_reads_file_base, sam_files, log)
if options_storage.stop_after == 'tpp':
support.finish_here(log)
#corrector
if "mismatch_corrector" in cfg and not options_storage.run_completed and \
(os.path.isfile(result_contigs_filename) or
(options_storage.continue_mode and os.path.isfile(assembled_contigs_filename))):
STAGE_NAME = "Mismatch correction"
to_correct = dict()
to_correct["contigs"] = (result_contigs_filename, assembled_contigs_filename)
if os.path.isfile(result_scaffolds_filename) or (options_storage.continue_mode and
os.path.isfile(assembled_scaffolds_filename)):
to_correct["scaffolds"] = (result_scaffolds_filename, assembled_scaffolds_filename)
# moving assembled contigs (scaffolds) to misc dir
for assembly_type, (old, new) in to_correct.items():
if options_storage.continue_mode and os.path.isfile(new):
continue
if os.path.isfile(old):
shutil.move(old, new)
if options_storage.continue_mode and os.path.isfile(result_contigs_filename) and \
(os.path.isfile(result_scaffolds_filename) or not os.path.isfile(assembled_scaffolds_filename)) \
and not options_storage.restart_from == 'mc':
log.info("\n===== Skipping %s (already processed). \n" % STAGE_NAME)
else:
if options_storage.restart_from == 'mc':
support.continue_from_here(log)
log.info("\n===== %s started." % STAGE_NAME)
# detecting paired-end library with the largest insert size
cfg["mismatch_corrector"].__dict__["dataset"] = cfg["dataset"].yaml_filename
#TODO: add reads orientation
import corrector_logic
corrector_cfg = cfg["mismatch_corrector"]
# processing contigs and scaffolds (or only contigs)
for assembly_type, (corrected, assembled) in to_correct.items():
if options_storage.continue_mode and os.path.isfile(corrected):
log.info("\n== Skipping processing of " + assembly_type + " (already processed)\n")
continue
support.continue_from_here(log)
log.info("\n== Processing of " + assembly_type + "\n")
tmp_dir_for_corrector = os.path.join(cfg["common"].output_dir, "mismatch_corrector", assembly_type)
cfg["mismatch_corrector"].__dict__["output_dir"] = tmp_dir_for_corrector
# correcting
corr_cfg = merge_configs(cfg["mismatch_corrector"], cfg["common"])
result_corrected_filename = os.path.join(tmp_dir_for_corrector, "corrected_contigs.fasta")
corrector_logic.run_corrector( tmp_configs_dir, bin_home, corr_cfg,
ext_python_modules_home, log, assembled, result_corrected_filename)
if os.path.isfile(result_corrected_filename):
shutil.copyfile(result_corrected_filename, corrected)
tmp_d = os.path.join(tmp_dir_for_corrector, "tmp")
if os.path.isdir(tmp_d) and not cfg["common"].developer_mode:
shutil.rmtree(tmp_d)
log.info("\n===== %s finished.\n" % STAGE_NAME)
if options_storage.stop_after == 'mc':
support.finish_here(log)
if not cfg["common"].developer_mode and os.path.isdir(tmp_configs_dir):
shutil.rmtree(tmp_configs_dir)
if not options_storage.run_completed:
#log.info("")
if "error_correction" in cfg and os.path.isdir(os.path.dirname(corrected_dataset_yaml_filename)):
log.info(" * Corrected reads are in " + support.process_spaces(os.path.dirname(corrected_dataset_yaml_filename) + "/"))
if "assembly" in cfg and os.path.isfile(result_contigs_filename):
message = " * Assembled contigs are in " + support.process_spaces(result_contigs_filename)
log.info(message)
if "assembly" in cfg and os.path.isfile(result_scaffolds_filename):
message = " * Assembled scaffolds are in " + support.process_spaces(result_scaffolds_filename)
log.info(message)
if "assembly" in cfg and os.path.isfile(result_assembly_graph_filename):
message = " * Assembly graph is in " + support.process_spaces(result_assembly_graph_filename)
log.info(message)
#log.info("")
#breaking scaffolds
if os.path.isfile(result_scaffolds_filename):
if not os.path.isdir(misc_dir):
os.makedirs(misc_dir)
result_broken_scaffolds = os.path.join(misc_dir, "broken_scaffolds.fasta")
if not os.path.isfile(result_broken_scaffolds) or not options_storage.continue_mode:
modified, broken_scaffolds = support.break_scaffolds(result_scaffolds_filename,
options_storage.THRESHOLD_FOR_BREAKING_SCAFFOLDS)
if modified:
support.write_fasta(result_broken_scaffolds, broken_scaffolds)
#log.info(" * Scaffolds broken by " + str(options_storage.THRESHOLD_FOR_BREAKING_SCAFFOLDS) +
# " Ns are in " + result_broken_scaffolds)
### printing WARNINGS SUMMARY
if not support.log_warnings(log):
log.info("\n======= SPAdes pipeline finished.") # otherwise it finished WITH WARNINGS
if options_storage.test_mode:
if options_storage.truseq_mode:
if not os.path.isfile(truseq_long_reads_file):
support.error("TEST FAILED: %s does not exist!" % truseq_long_reads_file)
else:
for result_filename in [result_contigs_filename, result_scaffolds_filename]:
if os.path.isfile(result_filename):
result_fasta = list(support.read_fasta(result_filename))
# correctness check: should be one contig of length 1000 bp
correct_number = 1
correct_length = 1000
if not len(result_fasta):
support.error("TEST FAILED: %s does not contain contigs!" % result_filename)
elif len(result_fasta) > correct_number:
support.error("TEST FAILED: %s contains more than %d contig (%d)!" %
(result_filename, correct_number, len(result_fasta)))
elif len(result_fasta[0][1]) != correct_length:
if len(result_fasta[0][1]) > correct_length:
relation = "more"
else:
relation = "less"
support.error("TEST FAILED: %s contains %s than %d bp (%d bp)!" %
(result_filename, relation, correct_length, len(result_fasta[0][1])))
else:
support.error("TEST FAILED: " + result_filename + " does not exist!")
log.info("\n========= TEST PASSED CORRECTLY.")
log.info("\nSPAdes log can be found here: " + log_filename)
log.info("")
log.info("Thank you for using SPAdes!")
log.removeHandler(log_handler)
except Exception:
exc_type, exc_value, _ = sys.exc_info()
if exc_type == SystemExit:
sys.exit(exc_value)
else:
if exc_type == OSError and exc_value.errno == errno.ENOEXEC: # Exec format error
support.error("It looks like you are using SPAdes binaries for another platform.\n" +
support.get_spades_binaries_info_message())
else:
log.exception(exc_value)
support.error("exception caught: %s" % exc_type, log)
except BaseException: # since python 2.5 system-exiting exceptions (e.g. KeyboardInterrupt) are derived from BaseException
exc_type, exc_value, _ = sys.exc_info()
if exc_type == SystemExit:
sys.exit(exc_value)
else:
log.exception(exc_value)
support.error("exception caught: %s" % exc_type, log)
def run_spades(configs_dir, execution_home, cfg, dataset_data, ext_python_modules_home, log):
if not isinstance(cfg.iterative_K, list):
cfg.iterative_K = [cfg.iterative_K]
cfg.iterative_K = sorted(cfg.iterative_K)
used_K = []
# checking and removing conflicting K-mer directories
if options_storage.restart_from and (options_storage.restart_k_mers != options_storage.original_k_mers):
processed_K = []
for k in range(options_storage.MIN_K, options_storage.MAX_K, 2):
cur_K_dir = os.path.join(cfg.output_dir, "K%d" % k)
if os.path.isdir(cur_K_dir) and os.path.isfile(os.path.join(cur_K_dir, "final_contigs.fasta")):
processed_K.append(k)
if processed_K:
RL = get_read_length(cfg.output_dir, processed_K[0], ext_python_modules_home, log)
needed_K = update_k_mers_in_special_cases(cfg.iterative_K, RL, log, silent=True)
needed_K = [k for k in needed_K if k < RL]
original_K = reveal_original_k_mers(RL)
k_to_delete = []
for id, k in enumerate(needed_K):
if len(processed_K) == id:
if processed_K[-1] == original_K[-1]: # the last K in the original run was processed in "last_one" mode
k_to_delete = [original_K[-1]]
break
if processed_K[id] != k:
k_to_delete = processed_K[id:]
break
if not k_to_delete and (len(processed_K) > len(needed_K)):
k_to_delete = processed_K[len(needed_K) - 1:]
if k_to_delete:
log.info("Restart mode: removing previously processed directories for K=%s "
"to avoid conflicts with K specified with --restart-from" % (str(k_to_delete)))
for k in k_to_delete:
shutil.rmtree(os.path.join(cfg.output_dir, "K%d" % k))
bin_reads_dir = os.path.join(cfg.output_dir, ".bin_reads")
if os.path.isdir(bin_reads_dir) and not options_storage.continue_mode:
shutil.rmtree(bin_reads_dir)
cfg.tmp_dir = support.get_tmp_dir(prefix="spades_")
finished_on_stop_after = False
K = cfg.iterative_K[0]
if len(cfg.iterative_K) == 1:
run_iteration(configs_dir, execution_home, cfg, log, K, None, True)
used_K.append(K)
else:
run_iteration(configs_dir, execution_home, cfg, log, K, None, False)
used_K.append(K)
if options_storage.stop_after == "k%d" % K:
finished_on_stop_after = True
else:
prev_K = K
RL = get_read_length(cfg.output_dir, K, ext_python_modules_home, log)
cfg.iterative_K = update_k_mers_in_special_cases(cfg.iterative_K, RL, log)
if len(cfg.iterative_K) < 2 or cfg.iterative_K[1] + 1 > RL:
if cfg.rr_enable:
if len(cfg.iterative_K) < 2:
log.info("== Rerunning for the first value of K (%d) with Repeat Resolving" %
cfg.iterative_K[0])
else:
support.warning("Second value of iterative K (%d) exceeded estimated read length (%d). "
"Rerunning for the first value of K (%d) with Repeat Resolving" %
(cfg.iterative_K[1], RL, cfg.iterative_K[0]), log)
run_iteration(configs_dir, execution_home, cfg, log, cfg.iterative_K[0], None, True)
used_K.append(cfg.iterative_K[0])
K = cfg.iterative_K[0]
else:
rest_of_iterative_K = cfg.iterative_K
rest_of_iterative_K.pop(0)
count = 0
for K in rest_of_iterative_K:
count += 1
last_one = count == len(cfg.iterative_K) or (rest_of_iterative_K[count] + 1 > RL)
run_iteration(configs_dir, execution_home, cfg, log, K, prev_K, last_one)
used_K.append(K)
prev_K = K
if last_one:
break
if options_storage.stop_after == "k%d" % K:
finished_on_stop_after = True
break
if count < len(cfg.iterative_K) and not finished_on_stop_after:
support.warning("Iterations stopped. Value of K (%d) exceeded estimated read length (%d)" %
(cfg.iterative_K[count], RL), log)
if options_storage.stop_after and options_storage.stop_after.startswith('k'):
support.finish_here(log)
latest = os.path.join(cfg.output_dir, "K%d" % K)
if cfg.correct_scaffolds and not options_storage.run_completed:
if options_storage.continue_mode and os.path.isfile(os.path.join(cfg.output_dir, "SCC", "corrected_scaffolds.fasta")) and not options_storage.restart_from == "scc":
log.info("\n===== Skipping %s (already processed). \n" % "scaffold correction")
else:
if options_storage.continue_mode:
support.continue_from_here(log)
run_scaffold_correction(configs_dir, execution_home, cfg, log, latest, 21)
latest = os.path.join(os.path.join(cfg.output_dir, "SCC"), "K21")
if options_storage.stop_after == 'scc':
support.finish_here(log)
if cfg.correct_scaffolds:
correct_scaffolds_fpath = os.path.join(latest, "corrected_scaffolds.fasta")
if os.path.isfile(correct_scaffolds_fpath):
shutil.copyfile(correct_scaffolds_fpath, cfg.result_scaffolds)
elif not finished_on_stop_after: # interupted by --stop-after, so final K is not processed!
if os.path.isfile(os.path.join(latest, "before_rr.fasta")):
result_before_rr_contigs = os.path.join(os.path.dirname(cfg.result_contigs), "before_rr.fasta")
if not os.path.isfile(result_before_rr_contigs) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "before_rr.fasta"), result_before_rr_contigs)
if options_storage.rna:
if os.path.isfile(os.path.join(latest, "transcripts.fasta")):
if not os.path.isfile(cfg.result_transcripts) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "transcripts.fasta"), cfg.result_transcripts)
if os.path.isfile(os.path.join(latest, "transcripts.paths")):
if not os.path.isfile(cfg.result_transcripts_paths) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "transcripts.paths"), cfg.result_transcripts_paths)
for filtering_type in options_storage.filtering_types:
prefix = filtering_type + "_filtered_"
result_filtered_transcripts = os.path.join(cfg.output_dir, prefix + options_storage.transcripts_name)
latest_filtered_transcripts = os.path.join(latest, prefix + "final_paths.fasta")
if os.path.isfile(latest_filtered_transcripts):
if not os.path.isfile(result_filtered_transcripts) or not options_storage.continue_mode:
shutil.copyfile(latest_filtered_transcripts, result_filtered_transcripts)
else:
if os.path.isfile(os.path.join(latest, "final_contigs.fasta")):
if not os.path.isfile(cfg.result_contigs) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "final_contigs.fasta"), cfg.result_contigs)
if os.path.isfile(os.path.join(latest, "first_pe_contigs.fasta")):
result_first_pe_contigs = os.path.join(os.path.dirname(cfg.result_contigs), "first_pe_contigs.fasta")
if not os.path.isfile(result_first_pe_contigs) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "first_pe_contigs.fasta"), result_first_pe_contigs)
if cfg.rr_enable:
if os.path.isfile(os.path.join(latest, "scaffolds.fasta")):
if not os.path.isfile(cfg.result_scaffolds) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "scaffolds.fasta"), cfg.result_scaffolds)
if os.path.isfile(os.path.join(latest, "scaffolds.paths")):
if not os.path.isfile(cfg.result_scaffolds_paths) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "scaffolds.paths"), cfg.result_scaffolds_paths)
if os.path.isfile(os.path.join(latest, "assembly_graph_with_scaffolds.gfa")):
if not os.path.isfile(cfg.result_graph_gfa) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "assembly_graph_with_scaffolds.gfa"), cfg.result_graph_gfa)
if os.path.isfile(os.path.join(latest, "assembly_graph.fastg")):
if not os.path.isfile(cfg.result_graph) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "assembly_graph.fastg"), cfg.result_graph)
if os.path.isfile(os.path.join(latest, "final_contigs.paths")):
if not os.path.isfile(cfg.result_contigs_paths) or not options_storage.continue_mode:
shutil.copyfile(os.path.join(latest, "final_contigs.paths"), cfg.result_contigs_paths)
if cfg.developer_mode:
# saves
saves_link = os.path.join(os.path.dirname(cfg.result_contigs), "saves")
if os.path.lexists(saves_link): # exists returns False for broken links! lexists return True
os.remove(saves_link)
os.symlink(os.path.join(latest, "saves"), saves_link)
if os.path.isdir(bin_reads_dir):
shutil.rmtree(bin_reads_dir)
if os.path.isdir(cfg.tmp_dir):
shutil.rmtree(cfg.tmp_dir)
return used_K
def main(args):
os.environ["LC_ALL"] = "C"
if len(args) == 1:
show_usage(0)
log = logging.getLogger('spades')
log.setLevel(logging.DEBUG)
console = logging.StreamHandler(sys.stdout)
console.setFormatter(logging.Formatter('%(message)s'))
console.setLevel(logging.DEBUG)
log.addHandler(console)
support.check_binaries(bin_home, log)
# auto detecting SPAdes mode (rna, meta, etc)
mode = options_storage.get_mode()
if mode is not None:
args.append('--' + mode)
# parse options and safe all parameters to cfg
options = args
cfg, dataset_data = fill_cfg(options, log)
if options_storage.continue_mode:
cmd_line, options = get_options_from_params(os.path.join(options_storage.output_dir, "params.txt"), args[0])
if not options:
support.error("failed to parse command line of the previous run! Please restart from the beginning or specify another output directory.")
cfg, dataset_data = fill_cfg(options, log, secondary_filling=True)
if options_storage.restart_from:
check_cfg_for_partial_run(cfg, type='restart-from')
options_storage.continue_mode = True
if options_storage.stop_after:
check_cfg_for_partial_run(cfg, type='stop-after')
log_filename = os.path.join(cfg["common"].output_dir, "spades.log")
if options_storage.continue_mode:
log_handler = logging.FileHandler(log_filename, mode='a')
else:
log_handler = logging.FileHandler(log_filename, mode='w')
log.addHandler(log_handler)
if options_storage.continue_mode:
log.info("\n======= SPAdes pipeline continued. Log can be found here: " + log_filename + "\n")
log.info("Restored from " + cmd_line)
if options_storage.restart_from:
updated_params = ""
skip_next = False
for v in args[1:]:
if v == '-o' or v == '--restart-from':
skip_next = True
continue
if skip_next or v.startswith('--restart-from='): # you can specify '--restart-from=k33' but not '-o=out_dir'
skip_next = False
continue
updated_params += "\t" + v
updated_params = updated_params.strip()
log.info("with updated parameters: " + updated_params)
cmd_line += "\t" + updated_params
log.info("")
params_filename = os.path.join(cfg["common"].output_dir, "params.txt")
params_handler = logging.FileHandler(params_filename, mode='w')
log.addHandler(params_handler)
if options_storage.continue_mode:
log.info(cmd_line)
else:
command = "Command line: "
for v in args:
# substituting relative paths with absolute ones (read paths, output dir path, etc)
v, prefix = support.get_option_prefix(v)
if v in options_storage.dict_of_rel2abs.keys():
v = options_storage.dict_of_rel2abs[v]
if prefix:
command += prefix + ":"
command += v + "\t"
log.info(command)
# special case
# if "mismatch_corrector" in cfg and not support.get_lib_ids_by_type(dataset_data, 'paired-end'):
# support.warning('cannot perform mismatch correction without at least one paired-end library! Skipping this step.', log)
# del cfg["mismatch_corrector"]
print_used_values(cfg, log)
log.removeHandler(params_handler)
support.check_single_reads_in_options(options, log)
if not options_storage.continue_mode:
log.info("\n======= SPAdes pipeline started. Log can be found here: " + log_filename + "\n")
# splitting interlaced reads and processing Ns in additional contigs if needed
if support.dataset_has_interlaced_reads(dataset_data) or support.dataset_has_additional_contigs(dataset_data)\
or support.dataset_has_nxmate_reads(dataset_data):
dir_for_split_reads = os.path.join(options_storage.output_dir, 'split_input')
if support.dataset_has_interlaced_reads(dataset_data) or support.dataset_has_nxmate_reads(dataset_data):
if not os.path.isdir(dir_for_split_reads):
os.makedirs(dir_for_split_reads)
if support.dataset_has_interlaced_reads(dataset_data):
dataset_data = support.split_interlaced_reads(dataset_data, dir_for_split_reads, log)
if support.dataset_has_nxmate_reads(dataset_data):
dataset_data = support.process_nxmate_reads(dataset_data, dir_for_split_reads, log)
if support.dataset_has_additional_contigs(dataset_data):
dataset_data = support.process_Ns_in_additional_contigs(dataset_data, dir_for_split_reads, log)
options_storage.dataset_yaml_filename = os.path.join(options_storage.output_dir, "input_dataset.yaml")
pyyaml.dump(dataset_data, open(options_storage.dataset_yaml_filename, 'w'))
cfg["dataset"].yaml_filename = options_storage.dataset_yaml_filename
try:
# copying configs before all computations (to prevent its changing at run time)
tmp_configs_dir = os.path.join(cfg["common"].output_dir, "configs")
if os.path.isdir(tmp_configs_dir) and not options_storage.continue_mode:
shutil.rmtree(tmp_configs_dir)
if not os.path.isdir(tmp_configs_dir):
if options_storage.configs_dir:
dir_util.copy_tree(options_storage.configs_dir, tmp_configs_dir, preserve_times=False, preserve_mode=False)
else:
dir_util.copy_tree(os.path.join(spades_home, "configs"), tmp_configs_dir, preserve_times=False, preserve_mode=False)
corrected_dataset_yaml_filename = ''
if "error_correction" in cfg:
STAGE_NAME = "Read error correction"
bh_cfg = merge_configs(cfg["error_correction"], cfg["common"])
corrected_dataset_yaml_filename = os.path.join(bh_cfg.output_dir, "corrected.yaml")
ec_is_needed = True
only_compressing_is_needed = False
if os.path.isfile(corrected_dataset_yaml_filename) and options_storage.continue_mode \
and not options_storage.restart_from == "ec":
if not bh_cfg.gzip_output or \
support.dataset_has_gzipped_reads(pyyaml.load(open(corrected_dataset_yaml_filename, 'r'))):
log.info("\n===== Skipping %s (already processed). \n" % STAGE_NAME)
ec_is_needed = False
else:
only_compressing_is_needed = True
if ec_is_needed:
if not only_compressing_is_needed:
support.continue_from_here(log)
if "HEAPCHECK" in os.environ:
del os.environ["HEAPCHECK"]
if "heap_check" in bh_cfg.__dict__:
os.environ["HEAPCHECK"] = bh_cfg.heap_check
if os.path.exists(bh_cfg.output_dir):
shutil.rmtree(bh_cfg.output_dir)
os.makedirs(bh_cfg.output_dir)
bh_cfg.__dict__["dataset_yaml_filename"] = cfg["dataset"].yaml_filename
log.info("\n===== %s started. \n" % STAGE_NAME)
hammer_logic.run_hammer(corrected_dataset_yaml_filename, tmp_configs_dir, bin_home, bh_cfg, dataset_data,
ext_python_modules_home, only_compressing_is_needed, log)
log.info("\n===== %s finished. \n" % STAGE_NAME)
if options_storage.stop_after == 'ec':
support.finish_here(log)
result_contigs_filename = os.path.join(cfg["common"].output_dir, options_storage.contigs_name)
result_scaffolds_filename = os.path.join(cfg["common"].output_dir, options_storage.scaffolds_name)
result_assembly_graph_filename = os.path.join(cfg["common"].output_dir, options_storage.assembly_graph_name)
result_contigs_paths_filename = os.path.join(cfg["common"].output_dir, options_storage.contigs_paths)
result_scaffolds_paths_filename = os.path.join(cfg["common"].output_dir, options_storage.scaffolds_paths)
result_transcripts_filename = os.path.join(cfg["common"].output_dir, options_storage.transcripts_name)
result_transcripts_paths_filename = os.path.join(cfg["common"].output_dir, options_storage.transcripts_paths)
truseq_long_reads_file_base = os.path.join(cfg["common"].output_dir, "truseq_long_reads")
truseq_long_reads_file = truseq_long_reads_file_base + ".fasta"
misc_dir = os.path.join(cfg["common"].output_dir, "misc")
### if mismatch correction is enabled then result contigs are copied to misc directory
assembled_contigs_filename = os.path.join(misc_dir, "assembled_contigs.fasta")
assembled_scaffolds_filename = os.path.join(misc_dir, "assembled_scaffolds.fasta")
if "assembly" in cfg and not options_storage.run_completed:
STAGE_NAME = "Assembling"
spades_cfg = merge_configs(cfg["assembly"], cfg["common"])
spades_cfg.__dict__["result_contigs"] = result_contigs_filename
spades_cfg.__dict__["result_scaffolds"] = result_scaffolds_filename
spades_cfg.__dict__["result_graph"] = result_assembly_graph_filename
spades_cfg.__dict__["result_contigs_paths"] = result_contigs_paths_filename
spades_cfg.__dict__["result_scaffolds_paths"] = result_scaffolds_paths_filename
spades_cfg.__dict__["result_transcripts"] = result_transcripts_filename
spades_cfg.__dict__["result_transcripts_paths"] = result_transcripts_paths_filename
if options_storage.continue_mode and (os.path.isfile(spades_cfg.result_contigs)
or ("mismatch_corrector" in cfg and
os.path.isfile(assembled_contigs_filename))
or (options_storage.truseq_mode and os.path.isfile(assembled_scaffolds_filename)))\
and not options_storage.restart_from == 'as' \
and not options_storage.restart_from == 'scc' \
and not (options_storage.restart_from and options_storage.restart_from.startswith('k')):
log.info("\n===== Skipping %s (already processed). \n" % STAGE_NAME)
# calculating latest_dir for the next stages
latest_dir = support.get_latest_dir(os.path.join(spades_cfg.output_dir, "K*"))
if not latest_dir:
support.error("failed to continue the previous run! Please restart from previous stages or from the beginning.", log)
else:
old_result_files = [result_contigs_filename, result_scaffolds_filename,
assembled_contigs_filename, assembled_scaffolds_filename]
for old_result_file in old_result_files:
if os.path.isfile(old_result_file):
os.remove(old_result_file)
if options_storage.restart_from == 'as':
support.continue_from_here(log)
if os.path.isfile(corrected_dataset_yaml_filename):
dataset_data = pyyaml.load(open(corrected_dataset_yaml_filename, 'r'))
dataset_data = support.relative2abs_paths(dataset_data, os.path.dirname(corrected_dataset_yaml_filename))
if spades_cfg.disable_rr:
spades_cfg.__dict__["rr_enable"] = False
else:
spades_cfg.__dict__["rr_enable"] = True
if "HEAPCHECK" in os.environ:
del os.environ["HEAPCHECK"]
if "heap_check" in spades_cfg.__dict__:
os.environ["HEAPCHECK"] = spades_cfg.heap_check
log.info("\n===== %s started.\n" % STAGE_NAME)
# creating dataset
dataset_filename = os.path.join(spades_cfg.output_dir, "dataset.info")
if not os.path.isfile(dataset_filename) or not options_storage.continue_mode:
dataset_file = open(dataset_filename, 'w')
import process_cfg
if os.path.isfile(corrected_dataset_yaml_filename):
dataset_file.write("reads" + '\t' + process_cfg.process_spaces(corrected_dataset_yaml_filename) + '\n')
else:
dataset_file.write("reads" + '\t' + process_cfg.process_spaces(cfg["dataset"].yaml_filename) + '\n')
if spades_cfg.developer_mode and "reference" in cfg["dataset"].__dict__:
dataset_file.write("reference_genome" + '\t')
dataset_file.write(process_cfg.process_spaces(cfg["dataset"].reference) + '\n')
dataset_file.close()
spades_cfg.__dict__["dataset"] = dataset_filename
used_K = spades_logic.run_spades(tmp_configs_dir, bin_home, spades_cfg, dataset_data, ext_python_modules_home, log)
if os.path.isdir(misc_dir) and not options_storage.continue_mode:
shutil.rmtree(misc_dir)
if not os.path.isdir(misc_dir):
os.makedirs(misc_dir)
if options_storage.continue_mode and options_storage.restart_from and options_storage.restart_from.startswith('k'):
k_str = options_storage.restart_from[1:]
if k_str.find(":") != -1:
k_str = k_str[:k_str.find(":")]
support.error("failed to continue from K=%s because this K was not processed in the original run!" % k_str, log)
log.info("\n===== %s finished. Used k-mer sizes: %s \n" % (STAGE_NAME, ', '.join(map(str, used_K))))
if not options_storage.run_completed:
if options_storage.stop_after == 'as' or options_storage.stop_after == 'scc' or (options_storage.stop_after and options_storage.stop_after.startswith('k')):
support.finish_here(log)
#postprocessing
if cfg["run_truseq_postprocessing"] and not options_storage.run_completed:
if options_storage.continue_mode and os.path.isfile(truseq_long_reads_file_base + ".fastq") and not options_storage.restart_from == 'tpp':
log.info("\n===== Skipping %s (already processed). \n" % "TruSeq postprocessing")
else:
support.continue_from_here(log)
if os.path.isfile(result_scaffolds_filename):
shutil.move(result_scaffolds_filename, assembled_scaffolds_filename)
reads_library = dataset_data[0]
alignment_bin = os.path.join(bin_home, "bwa-spades")
alignment_dir = os.path.join(cfg["common"].output_dir, "alignment")
sam_files = alignment.align_bwa(alignment_bin, assembled_scaffolds_filename, dataset_data, alignment_dir, log, options_storage.threads)
moleculo_postprocessing.moleculo_postprocessing(assembled_scaffolds_filename, truseq_long_reads_file_base, sam_files, log)
if options_storage.stop_after == 'tpp':
support.finish_here(log)
#corrector
if "mismatch_corrector" in cfg and not options_storage.run_completed and \
(os.path.isfile(result_contigs_filename) or
(options_storage.continue_mode and os.path.isfile(assembled_contigs_filename))):
STAGE_NAME = "Mismatch correction"
to_correct = dict()
to_correct["contigs"] = (result_contigs_filename, assembled_contigs_filename)
if os.path.isfile(result_scaffolds_filename) or (options_storage.continue_mode and
os.path.isfile(assembled_scaffolds_filename)):
to_correct["scaffolds"] = (result_scaffolds_filename, assembled_scaffolds_filename)
# moving assembled contigs (scaffolds) to misc dir
for assembly_type, (old, new) in to_correct.items():
if options_storage.continue_mode and os.path.isfile(new):
continue
if os.path.isfile(old):
shutil.move(old, new)
if options_storage.continue_mode and os.path.isfile(result_contigs_filename) and \
(os.path.isfile(result_scaffolds_filename) or not os.path.isfile(assembled_scaffolds_filename)) \
and not options_storage.restart_from == 'mc':
log.info("\n===== Skipping %s (already processed). \n" % STAGE_NAME)
else:
if options_storage.restart_from == 'mc':
support.continue_from_here(log)
log.info("\n===== %s started." % STAGE_NAME)
# detecting paired-end library with the largest insert size
cfg["mismatch_corrector"].__dict__["dataset"] = cfg["dataset"].yaml_filename
#TODO: add reads orientation
import corrector_logic
corrector_cfg = cfg["mismatch_corrector"]
# processing contigs and scaffolds (or only contigs)
for assembly_type, (corrected, assembled) in to_correct.items():
if options_storage.continue_mode and os.path.isfile(corrected):
log.info("\n== Skipping processing of " + assembly_type + " (already processed)\n")
continue
support.continue_from_here(log)
log.info("\n== Processing of " + assembly_type + "\n")
tmp_dir_for_corrector = os.path.join(cfg["common"].output_dir, "mismatch_corrector", assembly_type)
cfg["mismatch_corrector"].__dict__["output_dir"] = tmp_dir_for_corrector
# correcting
corr_cfg = merge_configs(cfg["mismatch_corrector"], cfg["common"])
result_corrected_filename = os.path.join(tmp_dir_for_corrector, "corrected_contigs.fasta")
corrector_logic.run_corrector( tmp_configs_dir, bin_home, corr_cfg,
ext_python_modules_home, log, assembled, result_corrected_filename)
if os.path.isfile(result_corrected_filename):
shutil.copyfile(result_corrected_filename, corrected)
tmp_d = os.path.join(tmp_dir_for_corrector, "tmp")
if os.path.isdir(tmp_d) and not cfg["common"].developer_mode:
shutil.rmtree(tmp_d)
log.info("\n===== %s finished.\n" % STAGE_NAME)
if options_storage.stop_after == 'mc':
support.finish_here(log)
if not cfg["common"].developer_mode and os.path.isdir(tmp_configs_dir):
shutil.rmtree(tmp_configs_dir)
if not options_storage.run_completed:
#log.info("")
if "error_correction" in cfg and os.path.isdir(os.path.dirname(corrected_dataset_yaml_filename)):
log.info(" * Corrected reads are in " + support.process_spaces(os.path.dirname(corrected_dataset_yaml_filename) + "/"))
if "assembly" in cfg and os.path.isfile(result_contigs_filename):
message = " * Assembled contigs are in " + support.process_spaces(result_contigs_filename)
log.info(message)
if options_storage.rna:
if "assembly" in cfg and os.path.isfile(result_transcripts_filename):
message = " * Assembled transcripts are in " + support.process_spaces(result_transcripts_filename)
log.info(message)
if "assembly" in cfg and os.path.isfile(result_transcripts_paths_filename):
message = " * Paths in the assembly graph corresponding to the transcripts are in " + \
support.process_spaces(result_transcripts_paths_filename)
log.info(message)
else:
if "assembly" in cfg and os.path.isfile(result_scaffolds_filename):
message = " * Assembled scaffolds are in " + support.process_spaces(result_scaffolds_filename)
log.info(message)
if "assembly" in cfg and os.path.isfile(result_assembly_graph_filename):
message = " * Assembly graph is in " + support.process_spaces(result_assembly_graph_filename)
log.info(message)
if "assembly" in cfg and os.path.isfile(result_contigs_paths_filename):
message = " * Paths in the assembly graph corresponding to the contigs are in " + \
support.process_spaces(result_contigs_paths_filename)
log.info(message)
if "assembly" in cfg and os.path.isfile(result_scaffolds_paths_filename):
message = " * Paths in the assembly graph corresponding to the scaffolds are in " + \
support.process_spaces(result_scaffolds_paths_filename)
log.info(message)
#log.info("")
#breaking scaffolds
if os.path.isfile(result_scaffolds_filename):
if not os.path.isdir(misc_dir):
os.makedirs(misc_dir)
result_broken_scaffolds = os.path.join(misc_dir, "broken_scaffolds.fasta")
if not os.path.isfile(result_broken_scaffolds) or not options_storage.continue_mode:
modified, broken_scaffolds = support.break_scaffolds(result_scaffolds_filename,
options_storage.THRESHOLD_FOR_BREAKING_SCAFFOLDS)
if modified:
support.write_fasta(result_broken_scaffolds, broken_scaffolds)
#log.info(" * Scaffolds broken by " + str(options_storage.THRESHOLD_FOR_BREAKING_SCAFFOLDS) +
# " Ns are in " + result_broken_scaffolds)
### printing WARNINGS SUMMARY
if not support.log_warnings(log):
log.info("\n======= SPAdes pipeline finished.") # otherwise it finished WITH WARNINGS
if options_storage.test_mode:
if options_storage.truseq_mode:
if not os.path.isfile(truseq_long_reads_file):
support.error("TEST FAILED: %s does not exist!" % truseq_long_reads_file)
elif options_storage.rna:
if not os.path.isfile(result_transcripts_filename):
support.error("TEST FAILED: %s does not exist!" % result_transcripts_filename)
else:
for result_filename in [result_contigs_filename, result_scaffolds_filename]:
if os.path.isfile(result_filename):
result_fasta = list(support.read_fasta(result_filename))
# correctness check: should be one contig of length 1000 bp
correct_number = 1
correct_length = 1000
if not len(result_fasta):
support.error("TEST FAILED: %s does not contain contigs!" % result_filename)
elif len(result_fasta) > correct_number:
support.error("TEST FAILED: %s contains more than %d contig (%d)!" %
(result_filename, correct_number, len(result_fasta)))
elif len(result_fasta[0][1]) != correct_length:
if len(result_fasta[0][1]) > correct_length:
relation = "more"
else:
relation = "less"
support.error("TEST FAILED: %s contains %s than %d bp (%d bp)!" %
(result_filename, relation, correct_length, len(result_fasta[0][1])))
else:
support.error("TEST FAILED: " + result_filename + " does not exist!")
log.info("\n========= TEST PASSED CORRECTLY.")
log.info("\nSPAdes log can be found here: " + log_filename)
log.info("")
log.info("Thank you for using SPAdes!")
log.removeHandler(log_handler)
except Exception:
exc_type, exc_value, _ = sys.exc_info()
if exc_type == SystemExit:
sys.exit(exc_value)
else:
if exc_type == OSError and exc_value.errno == errno.ENOEXEC: # Exec format error
support.error("It looks like you are using SPAdes binaries for another platform.\n" +
support.get_spades_binaries_info_message())
else:
log.exception(exc_value)
support.error("exception caught: %s" % exc_type, log)
except BaseException: # since python 2.5 system-exiting exceptions (e.g. KeyboardInterrupt) are derived from BaseException
exc_type, exc_value, _ = sys.exc_info()
if exc_type == SystemExit:
sys.exit(exc_value)
else:
log.exception(exc_value)
support.error("exception caught: %s" % exc_type, log)
