def __create_stock_rack_buffer_worklists(self):
# These worklists are responsible for the addition of annealing buffer
# to the pool stock rack. There is 1 worklist for each quadrant.
self.add_debug('Create stock rack buffer worklists ...')
buffer_volume = get_stock_pool_buffer_volume()
for sector_index, base_layout in self.__quadrant_layouts.iteritems():
label = self.LIBRARY_STOCK_BUFFER_WORKLIST_LABEL % (
self.library_name, (sector_index + 1))
self.__create_buffer_worklist(base_layout, buffer_volume, label,
sector_index)
def __write_pool_creation_section(self):
# This is the stock transfer part (creating pools from single molecule
# designs).
self.add_debug('Create pool section ...')
self._write_headline(header_text=self.HEADER_POOL_CREATION,
preceding_blank_lines=0)
lines = []
volume_line = self.VOLUME_LINE % (MOLECULE_DESIGN_TRANSFER_VOLUME)
volume_line += ' each'
lines.append(volume_line)
buffer_line = self.BUFFER_LINE % get_stock_pool_buffer_volume()
lines.append(buffer_line)
for sector_index in sorted(self.tube_destination_racks.keys()):
lines.append('')
lines.append(self.QUADRANT_LINE % (sector_index + 1))
barcodes = self.tube_destination_racks[sector_index]
target_rack = self.pool_stock_racks[sector_index]
src_line = self.SOURCE_LINE_PLURAL % (', '.join(sorted(barcodes)))
lines.append(src_line)
lines.append(self.TARGET_LINE % (target_rack))
self._write_body_lines(lines)
def __write_pool_creation_section(self):
# This is the stock transfer part (creating pools from single molecule
# designs).
self.add_debug('Create pool section ...')
self._write_headline(header_text=self.HEADER_POOL_CREATION,
preceding_blank_lines=0)
lines = []
volume_line = self.VOLUME_LINE % (MOLECULE_DESIGN_TRANSFER_VOLUME)
volume_line += ' each'
lines.append(volume_line)
buffer_line = self.BUFFER_LINE % get_stock_pool_buffer_volume()
lines.append(buffer_line)
for sector_index in sorted(self.tube_destination_racks.keys()):
lines.append('')
lines.append(self.QUADRANT_LINE % (sector_index + 1))
barcodes = self.tube_destination_racks[sector_index]
target_rack = self.pool_stock_racks[sector_index]
src_line = self.SOURCE_LINE_PLURAL % (', '.join(sorted(barcodes)))
lines.append(src_line)
lines.append(self.TARGET_LINE % (target_rack))
self._write_body_lines(lines)
