def _populate_iso(self, iso, layout):
StockSampleCreationIsoPopulator._populate_iso(self, iso, layout)
# Create sector preparation plates.
library_name = self.iso_request.label
ir_specs_96 = get_reservoir_specs_standard_96()
plate_specs_96 = get_rack_specs_from_reservoir_specs(ir_specs_96)
ir_specs_384 = get_reservoir_specs_standard_384()
plate_specs_384 = get_rack_specs_from_reservoir_specs(ir_specs_384)
future_status = get_item_status_future()
sec_layout_map = get_sector_layouts_for_384_layout(layout)
# Create preparation plates.
for sec_idx in range(NUMBER_SECTORS):
if not sec_idx in sec_layout_map:
continue
# TODO: Move label creation to LABELS class.
prep_label = self.PREP_PLATE_LABEL_PATTERN \
% (library_name,
iso.layout_number,
DEFAULT_PREPARATION_PLATE_CONCENTRATION,
sec_idx + 1)
prep_plate = plate_specs_96.create_rack(prep_label, future_status)
sec_layout = sec_layout_map[sec_idx]
iso.add_sector_preparation_plate(prep_plate, sec_idx,
sec_layout.create_rack_layout())
# Create aliquot plates.
for i in range(self.iso_request.number_aliquots):
# TODO: Move label creation to LABELS class.
aliquot_label = self.ALIQUOT_PLATE_LABEL_PATTERN \
% (library_name,
iso.layout_number,
DEFAULT_ALIQUOT_PLATE_CONCENTRATION,
i + 1)
aliquot_plate = plate_specs_384.create_rack(aliquot_label,
future_status)
iso.add_aliquot_plate(aliquot_plate)
def __sort_into_sectors(self):
# Create a rack layout for each quadrant.
self.add_debug("Sorting positions into sectors.")
self.__sector_layouts = get_sector_layouts_for_384_layout(self.base_layout, LibraryBaseLayout)
if len(self.__sector_layouts) < NUMBER_SECTORS:
missing_sector_labels = [str(si + 1) for si in range(NUMBER_SECTORS) if not si in self.__sector_layouts]
msg = "Some rack sectors are empty. You do not require stock " "racks for them: %s!" % ", ".join(
missing_sector_labels
)
self.add_warning(msg)
def __sort_into_sectors(self):
# Create a rack layout for each quadrant.
self.add_debug('Sorting positions into sectors.')
self.__sector_layouts = \
get_sector_layouts_for_384_layout(self.base_layout,
LibraryBaseLayout)
if len(self.__sector_layouts) < NUMBER_SECTORS:
missing_sector_labels = [str(si + 1)
for si in range(NUMBER_SECTORS)
if not si in self.__sector_layouts]
msg = 'Some rack sectors are empty. You do not require stock ' \
'racks for them: %s!' % ', '.join(missing_sector_labels)
self.add_warning(msg)
