def getDomains(self, sparkContext):
# recover the species name for using in temp files
self.species = Utils.getSpecies(self.source_path)
domainFinder = DomainFinder.DomainFinder()
# load source sequences into a single list
if ("fasta" in self.source_type):
list, file_content = Parsers.parseFastaToList(self.source_path, "")
elif ("genbank" in self.source_type):
list = Parsers.genBankToAminoacid(self.source_path)
print('Processing domains...')
# create RDD with source sequences
sourceRDD = sparkContext.parallelize(file_content, numSlices=2000)
if ("nucleotide" in self.source_type):
# execute sixFrame translation for each sequence in RDD
sourceRDD = sourceRDD.map(lambda x: SixFrameTranslator.main(x))
# execute Pfam domain prediction for each sixFrame translation in RDD
domainsRDD = sourceRDD.map(lambda x: domainFinder.main(x[0], x[1]))
processedRDD = domainsRDD.map(
lambda x: self.processDomainOutput(x[0], x[1]))
# recover Pfam domain prediction results from RDD
result = processedRDD.collectAsMap()
print('Done!')
return result
def genBankToAminoacid(path):
list = []
# only aminoacid sequence
translations = ''
files = []
if (os.path.isfile(path)):
files.append(path)
else:
files = Utils.listFilesExt(path, 'gbk')
for file in files:
species = Utils.getSpecies(file)
records = parseGenBank(file)
for record in records:
locus = record.id
for feature in record.features:
#if feature.key == "CDS":
if feature.type == "CDS":
id, locus_tag, gene, protein_id, translation, \
product, function, description = '','','','','','','',''
for key, value in feature.qualifiers.items():
# get rid of the quotes around the qualifier
# find entry ID
if key == "translation":
translation = value[0]
elif key == "gene":
gene = value[0]
elif key == "locus_tag":
locus_tag = value[0]
elif key == "protein_id":
protein_id = value[0]
protein_id = protein_id.replace('/', '')
elif key == "product":
product = value[0]
elif key == "function":
function = value[0]
#priority for gene ID
id = locus_tag if not id and len(locus_tag) > 1 else id
id = gene if not id and len(gene) > 1 else id
id = protein_id if not id and len(protein_id) > 1 else id
description = product if product.strip() else description
description += '|' + function if function.strip(
) else description
entry = '>' + locus + '|' + species + '|' + id + '|' + description + '\n' + translation
if (entry not in list):
list.append(entry)
translations += translation
return list, translations
def __init__(self, source_type=None, source_path=None, result_path=None):
self.config = Utils.loadConfig()
self.task = self.config.get('dataPipeline', 'task')
self.source_path = self.config.get(
'dataPipeline',
'source.path') if source_path is None else source_path
self.source_type = self.config.get(
'dataPipeline',
'source.type') if source_type is None else source_type
self.result_path = self.config.get(
'dataPipeline',
'result.path') if result_path is None else result_path
self.result_path = Utils.normalizePath(self.result_path)
# create if it doesnt exist
os.makedirs(os.path.dirname(self.result_path), exist_ok=True)
# recover the species name for using in temp files
self.species = Utils.getSpecies(self.source_path)
# temp dir + file used by sub-pipelines
self.path = os.path.dirname(os.path.realpath(__file__))
self.path += '/temp/'
os.makedirs(os.path.dirname(self.path), exist_ok=True)
