def extract_intra_spikes(DatFileName,IntraChannel,output_dir=None,ExtraChannels=None):
"""extracts spikes times from intracellular data"""
THRESH_FRAC = .5
DatFileName = os.path.abspath(DatFileName)
DatDir = os.path.dirname(DatFileName)
basename = intra_basename(DatFileName)
OutDir = join(output_dir,basename) if output_dir else join(DatDir,basename)
with indir(OutDir):
SpkFileName = basename+'.spk.1'
n_ch_dat,sample_rate = get_dat_pars(DatFileName)
global N_CH
N_CH = 1 if ExtraChannels is None else 1 + len(ExtraChannels)
set_globals_samples(sample_rate)
print("extracting intracellular spikes from %s"%DatFileName)
n_samples = num_samples(DatFileName,n_ch_dat,n_bytes=np.nbytes[DTYPE])
AllDataArr = np.memmap(DatFileName,dtype=np.int16,shape=(n_samples,n_ch_dat),mode='r')
b,a = signal.butter(3,100./(SAMPLE_RATE/2),'high') #filter at 100 Hz
IntraArr = AllDataArr[:,IntraChannel].copy()
IntraArr = signal.filtfilt(b,a,IntraArr)
Thresh = IntraArr.max()*THRESH_FRAC
Segs = contig_segs(np.flatnonzero(IntraArr > Thresh),padding=2)
TmList = map(lambda Seg: Seg[IntraArr[Seg].argmax()],Segs)
CluList = np.ones(len(TmList),dtype=np.int)
FetList = np.zeros((len(TmList),1),dtype=np.int)
SpkList = [get_padded(IntraArr,PeakSample-S_BEFORE,PeakSample+S_AFTER) for PeakSample in TmList]
SpkArr = np.array(SpkList)[:,:,np.newaxis]
if ExtraChannels is not None:
ExtraArr = AllDataArr[:,ExtraChannels].copy()
#b,a = signal.butter(BUTTER_ORDER,(F_LOW/(SAMPLE_RATE/2),.95),'pass')
ExtraArr = filtfilt2d(b,a,ExtraArr)
ExtraSpkList = [get_padded(ExtraArr,PeakSample-S_BEFORE,PeakSample+S_AFTER) for PeakSample in TmList]
ExtraSpkArr = np.array(ExtraSpkList)
SpkArr *= ExtraSpkArr[0].max()/SpkArr[0].max()
SpkArr = np.concatenate((np.array(ExtraSpkList),SpkArr),axis=2)
output.write_spk(np.array(SpkArr),SpkFileName)
write_files(basename,CluList,TmList,FetList,[])
def extract_wave_simple(IndList, FilteredArr):
IndArr = np.array(IndList, dtype=np.int32)
SampArr = IndArr[:, 0]
ChArr = IndArr[:, 1]
PeakSample = SampArr[FilteredArr[SampArr, ChArr].argmin()]
Wave = get_padded(FilteredArr, PeakSample - S_BEFORE, PeakSample + S_AFTER)
return Wave, PeakSample, bincount(ChArr, N_CH).astype(np.bool8)
def extract_wave_simple(IndList,FilteredArr):
IndArr = np.array(IndList,dtype=np.int32)
SampArr = IndArr[:,0]
ChArr = IndArr[:,1]
PeakSample = SampArr[FilteredArr[SampArr,ChArr].argmin()]
Wave = get_padded(FilteredArr,PeakSample-S_BEFORE,PeakSample+S_AFTER)
return Wave,PeakSample,bincount(ChArr,N_CH).astype(np.bool8)
def extract_wave_interp(IndList,FilteredArr):
IndArr = np.array(IndList,dtype=np.int32)
SampArr = IndArr[:,0]
ChArr = IndArr[:,1]
PeakInd = FilteredArr[SampArr,ChArr].argmin()
PeakSample,PeakChannel = SampArr[PeakInd],ChArr[PeakInd]
WavePlus = get_padded(FilteredArr,PeakSample-S_BEFORE-1,PeakSample+S_AFTER+1)
Wave = interp_around_peak(WavePlus,S_BEFORE+1,PeakChannel,S_BEFORE,S_AFTER,kind=INTERP_METHOD)
return Wave,PeakSample,bincount(ChArr,N_CH).astype(np.bool8)
def extract_wave_interp(IndList, FilteredArr):
IndArr = np.array(IndList, dtype=np.int32)
SampArr = IndArr[:, 0]
ChArr = IndArr[:, 1]
PeakInd = FilteredArr[SampArr, ChArr].argmin()
PeakSample, PeakChannel = SampArr[PeakInd], ChArr[PeakInd]
WavePlus = get_padded(FilteredArr, PeakSample - S_BEFORE - 1,
PeakSample + S_AFTER + 1)
Wave = interp_around_peak(WavePlus,
S_BEFORE + 1,
PeakChannel,
S_BEFORE,
S_AFTER,
kind=INTERP_METHOD)
return Wave, PeakSample, bincount(ChArr, N_CH).astype(np.bool8)
def extract_intra_spikes(DatFileName,
IntraChannel,
output_dir=None,
ExtraChannels=None):
"""extracts spikes times from intracellular data"""
THRESH_FRAC = .5
DatFileName = os.path.abspath(DatFileName)
DatDir = os.path.dirname(DatFileName)
basename = intra_basename(DatFileName)
OutDir = join(output_dir, basename) if output_dir else join(
DatDir, basename)
with indir(OutDir):
SpkFileName = basename + '.spk.1'
n_ch_dat, sample_rate = get_dat_pars(DatFileName)
global N_CH
N_CH = 1 if ExtraChannels is None else 1 + len(ExtraChannels)
set_globals_samples(sample_rate)
print("extracting intracellular spikes from %s" % DatFileName)
n_samples = num_samples(DatFileName,
n_ch_dat,
n_bytes=np.nbytes[DTYPE])
AllDataArr = np.memmap(DatFileName,
dtype=np.int16,
shape=(n_samples, n_ch_dat),
mode='r')
b, a = signal.butter(3, 100. / (SAMPLE_RATE / 2),
'high') #filter at 100 Hz
IntraArr = AllDataArr[:, IntraChannel].copy()
IntraArr = signal.filtfilt(b, a, IntraArr)
Thresh = IntraArr.max() * THRESH_FRAC
Segs = contig_segs(np.flatnonzero(IntraArr > Thresh), padding=2)
TmList = map(lambda Seg: Seg[IntraArr[Seg].argmax()], Segs)
CluList = np.ones(len(TmList), dtype=np.int)
FetList = np.zeros((len(TmList), 1), dtype=np.int)
SpkList = [
get_padded(IntraArr, PeakSample - S_BEFORE, PeakSample + S_AFTER)
for PeakSample in TmList
]
SpkArr = np.array(SpkList)[:, :, np.newaxis]
if ExtraChannels is not None:
ExtraArr = AllDataArr[:, ExtraChannels].copy()
#b,a = signal.butter(BUTTER_ORDER,(F_LOW/(SAMPLE_RATE/2),.95),'pass')
ExtraArr = filtfilt2d(b, a, ExtraArr)
ExtraSpkList = [
get_padded(ExtraArr, PeakSample - S_BEFORE,
PeakSample + S_AFTER) for PeakSample in TmList
]
ExtraSpkArr = np.array(ExtraSpkList)
SpkArr *= ExtraSpkArr[0].max() / SpkArr[0].max()
SpkArr = np.concatenate((np.array(ExtraSpkList), SpkArr), axis=2)
output.write_spk(np.array(SpkArr), SpkFileName)
write_files(basename, CluList, TmList, FetList, [])
