def waitpgm(self, sanitize=True):
if not self.ptcrunning:
return
#print "* Wait for PTC to finish"
if sanitize:
self.sanitize() # Sanitize tips before waiting for this to be done
worklist.comment("Wait for PTC")
while self.pgmEndTime-clock.pipetting > 120:
# Run any idle programs
oldElapsed=clock.pipetting
for ip in self.idlePgms:
if self.pgmEndTime-clock.pipetting > 120:
#print "Executing idle program with %.0f seconds"%(self.pgmEndTime-clock.pipetting)
ip(self.pgmEndTime-clock.pipetting-120)
if oldElapsed==clock.pipetting:
# Nothing was done
break
worklist.flushQueue() # Just in case
clock.pipandthermotime+=(clock.pipetting-self.pgmStartTime)
clock.thermotime+=(self.pgmEndTime-clock.pipetting)
clock.pipetting=self.pgmStartTime
#print "Waiting for PTC with %.0f seconds expected to remain"%(self.pgmEndTime-clock.pipetting)
self.lihahome()
worklist.pyrun('PTC\\ptcwait.py')
worklist.pyrun("PTC\\ptclid.py OPEN")
# worklist.pyrun('PTC\\ptcrun.py %s CALC ON'%"COOLDOWN")
# worklist.pyrun('PTC\\ptcwait.py')
worklist.vector("PTC200lid",decklayout.PTCPOS,worklist.SAFETOEND,True,worklist.DONOTMOVE,worklist.CLOSE)
worklist.vector("Hotel 1 Lid",decklayout.HOTELPOS,worklist.SAFETOEND,True,worklist.DONOTMOVE,worklist.OPEN)
worklist.vector("PTC200WigglePos",decklayout.PTCPOS,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle",decklayout.PTCPOS,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.CLOSE,True)
worklist.vector("PTC200Wiggle",decklayout.PTCPOS,worklist.ENDTOSAFE,False,worklist.DONOTMOVE,worklist.OPEN,True)
worklist.vector("PTC200WigglePos",decklayout.PTCPOS,worklist.ENDTOSAFE,False,worklist.DONOTMOVE,worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle2Pos",decklayout.PTCPOS,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle2",decklayout.PTCPOS,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.CLOSE,True)
worklist.vector("PTC200Wiggle2",decklayout.PTCPOS,worklist.ENDTOSAFE,False,worklist.DONOTMOVE,worklist.OPEN,True)
worklist.vector("PTC200Wiggle2Pos",decklayout.PTCPOS,worklist.ENDTOSAFE,False,worklist.DONOTMOVE,worklist.DONOTMOVE)
worklist.vector("PTC200WigglePos",decklayout.PTCPOS,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle",decklayout.PTCPOS,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.CLOSE,True)
worklist.vector("PTC200Wiggle",decklayout.PTCPOS,worklist.ENDTOSAFE,False,worklist.DONOTMOVE,worklist.OPEN,True)
worklist.vector("PTC200WigglePos",decklayout.PTCPOS,worklist.ENDTOSAFE,False,worklist.DONOTMOVE,worklist.DONOTMOVE)
self.ptcrunning=False
self.moveplate(decklayout.SAMPLEPLATE,"Home")
# Mark all samples on plate as unmixed (due to condensation)
Sample.notMixed(decklayout.SAMPLEPLATE.name)
# Verify plate is in place
worklist.vector(decklayout.SAMPLEPLATE.vectorName,decklayout.SAMPLEPLATE,worklist.SAFETOEND,False,worklist.DONOTMOVE,worklist.CLOSE)
worklist.vector(decklayout.SAMPLEPLATE.vectorName,decklayout.SAMPLEPLATE,worklist.ENDTOSAFE,False,worklist.OPEN,worklist.DONOTMOVE)
worklist.romahome()
#worklist.userprompt("Plate should be back on deck. Press return to continue")
# Wash tips again to remove any drips that may have formed while waiting for PTC
worklist.wash(15,1,5,True)
def sanitize(self,nmix=1,deepvol=20,force=False):
'Deep wash including RNase-Away treatment'
fixedTips=(~self.DITIMASK)&15
worklist.flushQueue()
if not force and fixedTips==self.cleanTips:
# print no sanitize needed
worklist.comment("Sanitize not needed cleanTips=%d"%self.cleanTips)
return
worklist.comment("Sanitize (cleanTips=%d)"%self.cleanTips)
worklist.wash(15,1,2)
fixedWells=[]
if not self.overrideSanitize:
for i in range(4):
if (fixedTips & (1<<i)) != 0:
fixedWells.append(i)
decklayout.BLEACH.addhistory("SANITIZE",0,1<<i)
worklist.mix(fixedTips,fixedWells,decklayout.BLEACH.mixLC,200,decklayout.BLEACH.plate,nmix,False)
worklist.wash(fixedTips,1,deepvol,True)
self.cleanTips|=fixedTips
# print "* Sanitize"
worklist.comment(clock.statusString())
def sanitize(self, nmix=1, deepvol=20, force=False):
'Deep wash including RNase-Away treatment'
fixedTips = (~self.DITIMASK) & 15
worklist.flushQueue()
if not force and fixedTips == self.cleanTips:
# print no sanitize needed
worklist.comment("Sanitize not needed cleanTips=%d" %
self.cleanTips)
return
worklist.comment("Sanitize (cleanTips=%d)" % self.cleanTips)
worklist.wash(15, 1, 2)
fixedWells = []
if not self.overrideSanitize:
for i in range(4):
if (fixedTips & (1 << i)) != 0:
fixedWells.append(i)
decklayout.BLEACH.addhistory("SANITIZE", 0, 1 << i)
worklist.mix(fixedTips, fixedWells, decklayout.BLEACH.mixLC, 200,
decklayout.BLEACH.plate, nmix, False)
worklist.wash(fixedTips, 1, deepvol, True)
self.cleanTips |= fixedTips
# print "* Sanitize"
worklist.comment(clock.statusString())
def waitpgm(self, sanitize=True):
if not self.ptcrunning:
return
#print "* Wait for PTC to finish"
if sanitize:
self.sanitize() # Sanitize tips before waiting for this to be done
worklist.comment("Wait for PTC")
while self.pgmEndTime - clock.pipetting > 120:
# Run any idle programs
oldElapsed = clock.pipetting
for ip in self.idlePgms:
if self.pgmEndTime - clock.pipetting > 120:
#print "Executing idle program with %.0f seconds"%(self.pgmEndTime-clock.pipetting)
ip(self.pgmEndTime - clock.pipetting - 120)
if oldElapsed == clock.pipetting:
# Nothing was done
break
worklist.flushQueue() # Just in case
clock.pipandthermotime += (clock.pipetting - self.pgmStartTime)
clock.thermotime += (self.pgmEndTime - clock.pipetting)
clock.pipetting = self.pgmStartTime
#print "Waiting for PTC with %.0f seconds expected to remain"%(self.pgmEndTime-clock.pipetting)
worklist.pyrun('PTC\\ptcwait.py')
worklist.pyrun("PTC\\ptclid.py OPEN")
# worklist.pyrun('PTC\\ptcrun.py %s CALC ON'%"COOLDOWN")
# worklist.pyrun('PTC\\ptcwait.py')
worklist.vector("PTC200lid", decklayout.PTCPOS, worklist.SAFETOEND,
True, worklist.DONOTMOVE, worklist.CLOSE)
worklist.vector("Hotel 1 Lid", decklayout.HOTELPOS, worklist.SAFETOEND,
True, worklist.DONOTMOVE, worklist.OPEN)
worklist.vector("PTC200WigglePos", decklayout.PTCPOS,
worklist.SAFETOEND, False, worklist.DONOTMOVE,
worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle", decklayout.PTCPOS, worklist.SAFETOEND,
False, worklist.DONOTMOVE, worklist.CLOSE, True)
worklist.vector("PTC200Wiggle", decklayout.PTCPOS, worklist.ENDTOSAFE,
False, worklist.DONOTMOVE, worklist.OPEN, True)
worklist.vector("PTC200WigglePos", decklayout.PTCPOS,
worklist.ENDTOSAFE, False, worklist.DONOTMOVE,
worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle2Pos", decklayout.PTCPOS,
worklist.SAFETOEND, False, worklist.DONOTMOVE,
worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle2", decklayout.PTCPOS, worklist.SAFETOEND,
False, worklist.DONOTMOVE, worklist.CLOSE, True)
worklist.vector("PTC200Wiggle2", decklayout.PTCPOS, worklist.ENDTOSAFE,
False, worklist.DONOTMOVE, worklist.OPEN, True)
worklist.vector("PTC200Wiggle2Pos", decklayout.PTCPOS,
worklist.ENDTOSAFE, False, worklist.DONOTMOVE,
worklist.DONOTMOVE)
worklist.vector("PTC200WigglePos", decklayout.PTCPOS,
worklist.SAFETOEND, False, worklist.DONOTMOVE,
worklist.DONOTMOVE)
worklist.vector("PTC200Wiggle", decklayout.PTCPOS, worklist.SAFETOEND,
False, worklist.DONOTMOVE, worklist.CLOSE, True)
worklist.vector("PTC200Wiggle", decklayout.PTCPOS, worklist.ENDTOSAFE,
False, worklist.DONOTMOVE, worklist.OPEN, True)
worklist.vector("PTC200WigglePos", decklayout.PTCPOS,
worklist.ENDTOSAFE, False, worklist.DONOTMOVE,
worklist.DONOTMOVE)
self.ptcrunning = False
self.moveplate(decklayout.SAMPLEPLATE, "Home")
# Mark all samples on plate as unmixed (due to condensation)
Sample.notMixed(decklayout.SAMPLEPLATE.name)
# Verify plate is in place
worklist.vector(decklayout.SAMPLEPLATE.vectorName,
decklayout.SAMPLEPLATE, worklist.SAFETOEND, False,
worklist.DONOTMOVE, worklist.CLOSE)
worklist.vector(decklayout.SAMPLEPLATE.vectorName,
decklayout.SAMPLEPLATE, worklist.ENDTOSAFE, False,
worklist.OPEN, worklist.DONOTMOVE)
worklist.romahome()
#worklist.userprompt("Plate should be back on deck. Press return to continue")
# Wash tips again to remove any drips that may have formed while waiting for PTC
worklist.wash(15, 1, 5, True)
