def writeKmersAndCounts(z, xs, nm=None):
if nm is None:
xNm = 'kmers'
cNm = 'counts'
else:
xNm = nm + '-kmers'
cNm = nm + '-counts'
t = tmpfile()
with open(t, 'w') as f:
ys = demuxKmersAndCounts(xs, f)
writeKmers(z, ys, xNm)
z.add_file(cNm, t)
os.remove(t)
def test_rwVector():
N = 65536
random.seed(17)
xs = [int(0.5 + 10 * random.expovariate(0.5)) for i in xrange(N)]
with autoremove():
t = tmpfile()
with casket(t, 'w') as z:
writeVector(z, xs, 'quux')
with casket(t, 'r') as z:
ys = readVectorList(z, 'quux')
assert len(xs) == len(ys)
for i in xrange(len(xs)):
assert xs[i] == ys[i]
def test_kmersList():
K = 25
M = (1 << (2 * K)) - 1
N = 65536
random.seed(17)
xs = [random.randint(0, M) for i in xrange(N)]
xs.sort()
with autoremove():
t = tmpfile()
with casket(t, 'w') as z:
zs = [x for x in xs]
writeKmersList(z, zs)
with casket(t, 'r') as z:
ys = list(readKmers(z))
assert len(xs) == len(ys)
for i in xrange(len(xs)):
assert xs[i] == ys[i], '%d\t%s\t%s' % (i, render(
K, xs[i]), render(K, ys[i]))
def mergeNinto(K, xss, hist, acgt, z, nm = None):
if nm is None:
knm = 'kmers'
cnm = 'counts'
else:
knm = nm + '-kmers'
cnm = nm + '-counts'
t = tmpfile()
with z.add_stream(knm) as kf, open(t, 'w') as cf:
with kmerWriter(kf) as kx, countsWriter(cf) as cx:
ss = [_kmerRadixBlockStream(K, xs) for xs in xss]
q = heap(ss)
while len(q) > 0:
v = q.front()
r = v.radix
ys = {}
while len(q) > 0 and v.radix == r:
for (x, c) in v.xs:
ys[x] = c + ys.get(x, 0)
v.next()
if v.done():
q.pop()
if len(q) > 0:
v = q.front()
else:
q.modifyfront()
v = q.front()
ys = ys.items()
ys.sort()
for (x, c) in ys:
hist[c] = 1 + hist.get(c, 0)
acgt[x&3] += c
kx.append(x)
cx.append(c)
z.add_file(cnm, t)
os.remove(t)
def __init__(self, z, zfn, comp):
self.z = z
self.zfn = zfn
self.comp = comp
self.tfn = tmpfile('.szf')
self.tf = open(self.tfn, 'w')
def main(argv):
opts = docopt.docopt(__doc__, argv)
K = 25
nms = []
idx = {}
for (nm, seq) in readFasta(openFile(opts['<baits>'])):
n = len(nms)
nms.append(nm)
for x in kmersList(K, seq, True):
if x not in idx:
idx[x] = set([])
idx[x].add(n)
for x in idx.keys():
idx[x] = list(idx[x])
idx[x].sort()
anti = set([])
if opts['-U']:
with openFile(opts['-U']) as f:
for (nm, seq) in readFasta(f):
for x in kmersList(K, seq, True):
anti.add(x)
rn = 0
if opts['-p']:
hist = {}
for (fn1, fn2) in pairs(opts['<input>']):
tmps = [(tmpfile('_1.fastq'), tmpfile('_2.fastq'))
for i in xrange(len(nms))]
cache = [[[], []] for i in xrange(len(nms))]
counts = [0 for i in xrange(len(nms))]
with openFile(fn1) as f1, openFile(fn2) as f2:
for fq1, fq2 in both(readFastq(f1), readFastq(f2)):
hits = set([])
pushup = False
for x in kmersList(K, fq1[1]):
if x in anti:
pushup = True
break
for i in idx.get(x, []):
hits.add(i)
for x in kmersList(K, fq2[1]):
if x in anti:
pushup = True
break
for i in idx.get(x, []):
hits.add(i)
if pushup:
continue
n = len(hits)
hist[n] = 1 + hist.get(n, 0)
for i in hits:
counts[i] += 1
cache[i][0].append(fq1)
cache[i][1].append(fq2)
if len(cache[i][0]) >= 1024:
with open(tmps[i][0], 'a') as f:
for rd in cache[i][0]:
print >> f, rd[0]
print >> f, rd[1]
print >> f, rd[2]
print >> f, rd[3]
with open(tmps[i][1], 'a') as f:
for rd in cache[i][1]:
print >> f, rd[0]
print >> f, rd[1]
print >> f, rd[2]
print >> f, rd[3]
cache[i][0] = []
cache[i][1] = []
for i in xrange(len(cache)):
if len(cache[i][0]) > 0:
with open(tmps[i][0], 'a') as f:
for rd in cache[i][0]:
print >> f, rd[0]
print >> f, rd[1]
print >> f, rd[2]
print >> f, rd[3]
with open(tmps[i][1], 'a') as f:
for rd in cache[i][1]:
print >> f, rd[0]
print >> f, rd[1]
print >> f, rd[2]
print >> f, rd[3]
cache[i][0] = []
cache[i][1] = []
with zipfile.ZipFile(opts['<output>'], 'w',
zipfile.ZIP_DEFLATED) as z:
for i in xrange(len(nms)):
if counts[i] > 0:
pth = '/'.join(nms[i].split())
z.write(tmps[i][0], pth + '/' + fn1)
os.remove(tmps[i][0])
z.write(tmps[i][1], pth + '/' + fn2)
os.remove(tmps[i][1])
hist = hist.items()
hist.sort()
for (n, f) in hist:
print '%d\t%d' % (n, f)
else:
raise "not implemented"
def main(argv):
opts = docopt.docopt(__doc__, argv)
verbose = opts['-v']
K = int(opts['<k>'])
out = opts['<output>']
Z = 1024 * 1024 * 32
if opts['-m'] is not None:
Z = 1024 * 1024 * int(opts['-m'])
buf = KmerAccumulator2(K)
n = 0
tmps = []
acgt = [0, 0, 0, 0]
m = 0
d = None
if opts['-D'] is not None:
d = float(opts['-D'])
S = 0
if opts['-S'] is not None:
S = int(opts['-S'])
cacheYes = set([])
cacheNo = set([])
B = opts['-C']
if B is not None:
xs = set([])
for (nm, seq) in readFasta(openFile(B)):
xs |= set(kmersList(K, seq, True))
B = xs
tmpnm = tmpfile('.pmc')
with casket(tmpnm, 'w') as z:
nr = 0
for itm in reads(opts['<input>'],
K=K,
pairs=False,
reads=False,
kmers=True,
both=True,
verbose=verbose):
xs = itm.kmers[0]
for x in xs:
acgt[x & 3] += 1
if d is not None:
for x in xs:
if x in cacheNo:
continue
if x not in cacheYes:
if not sub(S, d, x):
cacheNo.add(x)
continue
cacheYes.add(x)
buf.add(x)
m += 1
n += 1
if len(cacheYes) > 1000000:
cacheYes = set([])
if len(cacheNo) > 1000000:
cacheNo = set([])
elif B is not None:
found = False
for x in xs:
if x in B:
found = True
break
if found:
buf.addList(xs)
for x in xs:
m += 1
n += 1
else:
buf.addList(xs)
for x in xs:
m += 1
n += 1
nr += 1
if (nr & 1023) == 0 and buf.mem() >= Z // 2:
fn = 'tmps-%d' % (len(tmps), )
tmps.append(fn)
writeKmersAndCounts2(z, buf.kmersOnly(), buf.countsOnly(), fn)
buf.clear()
n = 0
if len(tmps) and len(buf):
fn = 'tmps-%d' % (len(tmps), )
tmps.append(fn)
writeKmersAndCounts2(z, buf.kmersOnly(), buf.countsOnly(), fn)
buf = []
with zotk.kmers(out, 'w') as z:
h = {}
if len(tmps) == 0:
for c in buf.countsOnly():
h[c] = 1 + h.get(c, 0)
writeKmersAndCounts2(z, buf.kmersOnly(), buf.countsOnly())
elif len(tmps) == 1:
with casket(tmpnm, 'r') as z0:
writeKmersAndCounts(z, readKmersAndCounts(z0, tmps[0]))
else:
with casket(tmpnm, 'r') as z0:
xss = [readKmersAndCounts(z0, t) for t in tmps]
mergeNinto(K, xss, h, z)
n = float(sum(acgt))
acgt = [c / n for c in acgt]
z.meta['K'] = K
z.meta['kmers'] = 'kmers'
z.meta['counts'] = 'counts'
z.meta['hist'] = h
z.meta['acgt'] = acgt
z.meta['reads'] = nr
os.remove(tmpnm)
def main(argv):
opts = docopt.docopt(__doc__, argv)
K = None
out = opts['<output>']
px = list(pairs(opts['<input>']))
if len(px) == 1:
with kmers(out, 'w') as z:
h = {}
acgt = [0, 0, 0, 0]
ix = px[0]
if len(ix) == 1:
with kmers(ix[0], 'r') as z0:
K = z0.meta['K']
xs = readKmersAndCounts(z0)
zs = hist(xs, h, acgt)
writeKmersAndCounts(z, xs)
else:
with kmers(ix[0], 'r') as z0, kmers(ix[1], 'r') as z1:
K = z0.meta['K']
K1 = z1.meta['K']
if K1 != K:
print >> sys.stderr, "mismatched K"
sys.exit(1)
xs = readKmersAndCounts(z0)
ys = readKmersAndCounts(z1)
zs = hist(merge(xs, ys), h, acgt)
writeKmersAndCounts(z, zs)
n = float(sum(acgt))
acgt = [c/n for c in acgt]
z.meta['hist'] = h
z.meta['acgt'] = acgt
return
tmps = []
tmpnm = tmpfile('.pmc')
with casket(tmpnm, 'w') as z:
for ix in px:
if len(ix) == 1:
nm = 'tmp-' + str(len(tmps))
tmps.append(nm)
with kmers(ix[0], 'r') as z0:
if K is None:
K = z0.meta['K']
else:
K0 = z0.meta['K']
if K0 != K:
print >> sys.stderr, "mismatched K"
sys.exit(1)
xs = readKmersAndCounts(z0)
writeKmersAndCounts(z, xs, nm)
else:
nm = 'tmp-' + str(len(tmps))
tmps.append(nm)
with kmers(ix[0], 'r') as z0, kmers(ix[1], 'r') as z1:
if K is None:
K = z0.meta['K']
else:
K0 = z0.meta['K']
if K0 != K:
print >> sys.stderr, "mismatched K"
sys.exit(1)
K1 = z1.meta['K']
if K1 != K:
print >> sys.stderr, "mismatched K"
sys.exit(1)
xs = readKmersAndCounts(z0)
ys = readKmersAndCounts(z1)
writeKmersAndCounts(z, merge(xs, ys), nm)
assert K is not None
with kmers(out, 'w') as z:
h = {}
acgt = [0, 0, 0, 0]
with casket(tmpnm, 'r') as z0:
xss = [readKmersAndCounts(z0, t) for t in tmps]
mergeNinto(K, xss, h, acgt, z)
n = float(sum(acgt))
acgt = [c/n for c in acgt]
z.meta['K'] = K
z.meta['kmers'] = 'kmers'
z.meta['counts'] = 'counts'
z.meta['hist'] = h
z.meta['acgt'] = acgt
os.remove(tmpnm)
