def boolean_gating(gate_params):
points = gate_params['points']
points2 = gate_params['1']
boolean_op = gate_params['0']
reverse_gate = '0'
if 'reverse' in gate_params:
if (gate_params['reverse']):
reverse_gate = '1'
## Generate unique datastore path, ensuring uniqueness.
while True:
new_name = str(uuid1())
new_path = ds.generate_path(DATA_BUCKET, None, new_name)
if not ds.check_exists(new_path, None):
break
if (gate_params['tool'] == "boolean_gating"):
if (len(points)%2 == 0) and (len(points2)%2 == 0):
if (reverse_gate == '1'):
if (boolean_op == 'or'):
boolean_op = 'and'
elif (boolean_op == 'and'):
boolean_op = 'or'
else:
return generate_gating_feedback("fail", "The boolean operator must be either 'and' or 'or'")
gate1_points = " ".join(str(p) for p in points)
gate2_points = " ".join(str(p) for p in points2)
queue.gate_boolean(gate_params['filename'], new_name, boolean_op, 'poly', gate1_points, reverse_gate, 'poly', gate2_points, reverse_gate, gate_params['axes']['x'], gate_params['axes']['y'], gate_params['axes']['x'], gate_params['axes']['y']);
return generate_gating_feedback("success", "the boolean gating was performed correctly", new_name, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect, wrong number of arguments")
def multiple_gating(gate_params):
points = gate_params['points']
while True:
new_name = str(uuid1())
new_path = ds.generate_path(DATA_BUCKET, None, new_name)
if not ds.check_exists(new_path, None):
break
if (gate_params['tool'] == 'kmeans_gating') :
if len(points) == 1 :
clusters = new_name
number_gates = points[0]
new_names = [new_name]
for i in range(0, number_gates-1):
while True:
next_new_name = str(uuid1())
path = ds.generate_path(DATA_BUCKET, None, next_new_name)
if not ds.check_exists(path, None):
new_names.append(next_new_name)
clusters = next_new_name + " " + clusters
break
queue.gate_kmeans(gate_params['filename'], clusters, str(number_gates), gate_params['axes']['x'], gate_params['axes']['y']);
return generate_gating_feedback("success", "the kmeans gate was performed correctly", new_names, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect, wrong number of arguments")
elif (gate_params['tool'] == 'quadrant_gating') :
if len(points) == 2 :
other_new_names = []
for i in range(0, 3):
while True:
next_new_name = str(uuid1())
path = ds.generate_path(DATA_BUCKET, None, next_new_name)
if not ds.check_exists(path, None):
other_new_names.append(next_new_name)
break
x_coord = str(points[0])
y_coord = str(points[1])
queue.gate_quadrant(gate_params['filename'], x_coord, y_coord, new_name, other_new_names[0], other_new_names[1], other_new_names[2], gate_params['axes']['x'], gate_params['axes']['y']);
new_paths = [new_name] + other_new_names
new_paths.reverse() #Make sure the user polls for the last graph to be created
return generate_gating_feedback("success", "the quadrant gate was performed correctly", new_paths, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect, wrong number of arguments")
def analysis_status_json(request):
authed_user = auth.get_current_user()
if authed_user is None:
return __unauthed_response()
user_key = ps.get_user_key_by_id(authed_user.user_id())
response_part = {
'backoff' : 0, #tells the client to back off for a given amount of time (milliseconds) (This is added to the client's constant poll interval)
'giveup' : True, #True instructs the client to stop polling - useful for situations such as unauthed requests where polling will never result in the user being shown a graph
'done' : False #True indicates that the analysis has finished and that the user can be redirected to the new image
}
try:
file_req = json.loads(request.raw_post_data)
except ValueError:
response_part.update({'error' : 'Invalid request payload.'})
return HttpResponse(json.dumps(response_part), content_type="application/json")
if 'filename' not in file_req:
response_part.update({'error' : 'Incomplete request.'})
return HttpResponse(json.dumps(response_part), content_type="application/json")
chk_file_name = DATA_BUCKET + '/' + file_req['filename']
file_entry = ps.get_file_by_name(chk_file_name)
logging.info(file_entry)
if file_entry is None:
response_part.update( { 'error' : 'File or gate not recognised.', 'giveup' : False } )
return HttpResponse(json.dumps(response_part), content_type="application/json")
else:
fp_entry = ps.get_user_file_permissions(file_entry.key, user_key)
if fp_entry is None:
response_part.update( { 'error' : 'Permission denied.' } )
return HttpResponse(json.dumps(response_part), content_type="application/json")
name = file_req['filename']
is_done = ds.check_exists(GRAPH_BUCKET + '/' + name + '.png', None)
#Prevent redirecting before the view is ready
is_done &= ds.check_exists(DATA_BUCKET + '/' + name, None)
#Prevent redirecting before gating info is ready
is_done &= ds.check_exists(INFO_BUCKET + '/' + name + '.txt', None)
response_part.update( { 'done' : is_done, 'giveup' : False } )
return HttpResponse(json.dumps(response_part), content_type="application/json")
def new_file(self, field_name, file_name, content_type, content_length, charset):
## Generate unique datastore path, ensuring uniqueness.
while True:
self.name = str(uuid1())
path = ds.generate_path(buckets.DATA + "/", None, self.name)
if not ds.check_exists(path, None):
break
## Generate friendly name.
friendly_name = splitext(file_name)[0]
## Setup file handle.
self.file_handle = ds.add_file(path, friendly_name, "raw_data", "w")
## Setup uploaded file.
self.upload = fcsUploadedFile(path, self.name, content_type, charset)
return None
def change_axes(tool_params):
## Generate unique datastore path, ensuring uniqueness.
while True:
new_name = str(uuid1())
new_path = ds.generate_path(DATA_BUCKET, None, new_name)
if not ds.check_exists(new_path, None):
break
if tool_params['newAxes']['x'] == tool_params['newAxes']['y']:
return generate_gating_feedback("fail", "Please choose two different axes")
# todo: check if the axes exist.
queue.change_axis(tool_params['filename'], tool_params['newAxes']['x'], tool_params['newAxes']['y'], new_name)
return generate_gating_feedback("success","the axis change was performed", new_name, tool_params['filename'], tool_params['newAxes']['x'], tool_params['newAxes']['y'])
def file_preview(request, file = None):
app_access = __check_app_access()
if app_access is not None:
return app_access
## Authentication.
authed_user = auth.get_current_user()
if authed_user is None:
authed_user_nick = 'Guest'
else:
authed_user_nick = authed_user.nickname()
## Graph visualisation.
# Replaced by a spinning canvas on the clientside
# if not ds.check_exists(GRAPH_BUCKET + '/' + file_name_without_extension + '.png', None):
# file_name_without_extension = None
#TODO: Might need to be simplified or moved to a function in fileinfo
# TODO the folowing should be replaced by a method in the APIDatastore
file_info = ps.get_file_by_name(DATA_BUCKET + '/' + file)
undo_uri = None
if file_info is not None:
prev_file = ps.get_file_by_key(file_info.prev_file_key)
if prev_file is not None:
undo_uri = prev_file.file_name.rpartition(DATA_BUCKET + '/')[2]
lst = ds.list(DATA_BUCKET)
current_file = None
for temp_file in lst:
temp_file.filename = temp_file.filename.rpartition('/')[2]
if temp_file.filename == file:
current_file = temp_file;
# Check whether graph exists yet.
graph_exists = ds.check_exists(GRAPH_BUCKET + '/' + file.partition('.')[0] + '.png', None)
# Get permissions for file.
permissions = None
if file_info is not None:
user_key = ps.get_user_key_by_id(authed_user.user_id())
permissions = ps.get_user_file_permissions(file_info.key, user_key)
template_dict = {'current_file' : current_file,
'name' : file,
'authed_user_nick': authed_user_nick,
'file_info' : file_info,
'graph_ready' : graph_exists,
'undo_link' : undo_uri,
'permissions' : permissions }
## Get gating information.
info_path = INFO_BUCKET + '/' + file.partition('.')[0] + '.txt'
if ds.check_exists(info_path, None):
buffer = ds.open(info_path)
if buffer:
file_text = buffer.read()
stats = file_text.split(' ')
if len(stats)>=3:
template_dict.update( { 'gating_stats' : { 'selection' : stats[0],
'total' : stats[1],
'percent' : float(stats[2])*100 } } )
## Get different axis
axis_path = INFO_BUCKET + '/' + file.partition('.')[0] + 'info.txt'
if ds.check_exists(axis_path, None):
buffer = ds.open(axis_path)
if buffer:
file_text = buffer.read()
axis = file_text.split('\n')
if len(axis)>0:
while '' in axis:
axis.remove('')
template_dict.update( { 'available_axes' : axis } )
return render(request, 'file_preview.html', template_dict)
def test_checkexists_notExistDir(self):
self.assertFalse(ds.check_exists("/some_bucket/not_a_real_dir/", None))
def test_checkexists_notExist(self):
self.assertFalse(ds.check_exists("/some_bucket/no_file/here/file.notafile", None))
def test_addfile_checkExists(self):
ds.add_file("/test_bucket/test_dir/test_file.ext")
self.assertTrue(ds.check_exists("/test_bucket/test_dir/test_file.ext", None))
def simple_gating(gate_params):
points = gate_params['points']
reverse_gate = '0'
if 'reverse' in gate_params:
if (gate_params['reverse']):
reverse_gate = '1'
## Generate unique datastore path, ensuring uniqueness.
while True:
new_name = str(uuid1())
new_path = ds.generate_path(DATA_BUCKET, None, new_name)
if not ds.check_exists(new_path, None):
break
if (gate_params['tool'] == "rectangular_gating") :
if len(points) == 4 :
#Reoder the point to take the topLeft and bottomRight points of the square
if points[0] > points[2]:
tempcoor = points[0]
points[0] = points[2]
points[2] = tempcoor
if points[1] > points[3]:
tempcoor = points[1]
points[1] = points[3]
points[3] = tempcoor
gating_request = " ".join(str(p) for p in points)
queue.gate_rectangle(gate_params['filename'], gating_request, new_name, reverse_gate, gate_params['axes']['x'], gate_params['axes']['y']);
return generate_gating_feedback("success", "the rectangular gating was performed correctly", new_name, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect " + params + " length:" + str(len(points)) + " is not equal to 4")
elif (gate_params['tool'] == "polygon_gating") :
if len(points)%2 == 0 :
gating_request = " ".join(str(p) for p in points)
queue.gate_polygon(gate_params['filename'], gating_request, new_name, reverse_gate, gate_params['axes']['x'], gate_params['axes']['y']);
return generate_gating_feedback("success", "the polygonal gating was performed correctly", new_name, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect " + params + " #pointCoordinates:" + str(len(points))-1 + " is not pair")
elif (gate_params['tool'] == "oval_gating") :
if len(points) == 6 :
gating_request = " ".join(str(p) for p in points)
queue.gate_circle(gate_params['filename'], gating_request, new_name, reverse_gate, gate_params['axes']['x'], gate_params['axes']['y']);
return generate_gating_feedback("success", "the oval gating was performed correctly", new_name, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect " + params + " #pointCoordinates:" + str(len(points)) + " is not even")
elif (gate_params['tool'] == 'normal_gating') :
if len(points) == 1 :
gating_request = str(points[0])
queue.gate_normal(gate_params['filename'], new_name, reverse_gate, gate_params['axes']['x'], gate_params['axes']['y'], gating_request);
return generate_gating_feedback("success", "the normal gating was performed correctly", new_name, gate_params['filename'], gate_params['axes']['x'], gate_params['axes']['y'])
else:
return generate_gating_feedback("fail", "notcorrect, wrong number of arguments")
else :
return generate_gating_feedback("fail", "The gate " + gate_params['tool'] + " is not known")
