def t_unknown_seq(self):
"""Prepare unknown base sequences with the correct length.
"""
gff_iterator = GFFAddingIterator()
rec_dict = gff_iterator.get_all_features(self._test_gff_file)
assert len(rec_dict["I"].seq) == 12766937
assert len(rec_dict["X"].seq) == 17718531
def t_fasta_directive(self):
"""Parse FASTA sequence information contained in a GFF3 file.
"""
iterator = GFFAddingIterator()
recs = iterator.get_all_features(self._gff_file)
assert len(recs) == 1
test_rec = recs['chr17']
assert str(test_rec.seq) == "GATTACAGATTACA"
def t_ensembl_nested_features(self):
"""Test nesting of features with GFF2 files using transcript_id.
"""
gff_iterator = GFFAddingIterator()
rec_dict = gff_iterator.get_all_features(self._ensembl_file)
assert len(rec_dict["I"].features) == 2
t_feature = rec_dict["I"].features[0]
assert len(t_feature.sub_features) == 32
def t_gff3_noval_attrib(self):
"""Parse GFF3 file from NCBI with a key/value pair with no value.
"""
gff_iterator = GFFAddingIterator()
recs = gff_iterator.get_all_features(self._test_ncbi)
assert len(recs) == 1
t_feature = recs.values()[0].features[0]
assert t_feature.qualifiers["pseudo"] == ["true"]
def t_gff2_iteration(self):
"""Test iterated features with GFF2 files, breaking without parents.
"""
gff_iterator = GFFAddingIterator()
break_dicts = []
for rec_dict in gff_iterator.get_features(self._wormbase_file,
target_lines=15):
break_dicts.append(rec_dict)
assert len(break_dicts) == 3
def t_solid_iterator(self):
"""Iterated parsing in a flat file without nested features.
"""
gff_iterator = GFFAddingIterator()
feature_sizes = []
for rec_dict in gff_iterator.get_features(self._test_gff_file,
target_lines=5):
feature_sizes.append([len(r.features) for r in rec_dict.values()])
assert max([sum(s) for s in feature_sizes]) == 5
assert len(feature_sizes) == 26, len(feature_sizes)
def t_gff3_multiple_ids(self):
"""Deal with GFF3 with non-unique ID attributes, using NCBI example.
"""
gff_iterator = GFFAddingIterator()
recs = gff_iterator.get_all_features(self._test_ncbi)
assert len(recs) == 1
t_features = recs.values()[0].features[1:]
# 4 feature sets, same ID, different positions, different attributes
assert len(t_features) == 4
for f in t_features:
assert len(f.sub_features) == 3
def t_gff3_iterator(self):
"""Iterated parsing in GFF3 files with nested features.
"""
gff_iterator = GFFAddingIterator()
feature_sizes = []
for rec_dict in gff_iterator.get_features(self._test_gff_file,
target_lines=70):
feature_sizes.append([len(r.features) for r in rec_dict.values()])
# should be one big set because we don't have a good place to split
assert len(feature_sizes) == 1
assert feature_sizes[0][0] == 59
def t_basic_directives(self):
"""Parse out top level meta-data supplied in a GFF3 file.
"""
iterator = GFFAddingIterator()
recs = iterator.get_all_features(self._gff_file)
anns = recs['chr17'].annotations
assert anns['gff-version'] == ['3']
assert anns['attribute-ontology'] == ['baz']
assert anns['feature-ontology'] == ['bar']
assert anns['source-ontology'] == ['boo']
assert anns['sequence-region'] == [('foo', '1', '100'), ('chr17',
'62467934', '62469545')]
def t_wormbase_nested_features(self):
"""Test nesting of features with GFF2 files using Transcript only.
"""
gff_iterator = GFFAddingIterator()
rec_dict = gff_iterator.get_all_features(self._wormbase_file)
assert len(rec_dict) == 3
parent_features = [f for f in rec_dict["I"].features if f.type ==
"Transcript"]
assert len(parent_features) == 1
inferred_features = [f for f in rec_dict["I"].features if f.type ==
"inferred_parent"]
assert len(inferred_features) == 0
tfeature = parent_features[0]
assert tfeature.qualifiers["WormPep"][0] == "WP:CE40797"
assert len(tfeature.sub_features) == 46
def t_line_adjust(self):
"""Adjust lines during parsing to fix potential GFF problems.
"""
def adjust_fn(results):
rec_index = results['quals']['i'][0]
read_name = results['rec_id']
results['quals']['read_name'] = [read_name]
results['rec_id'] = rec_index
return results
gff_iterator = GFFAddingIterator(line_adjust_fn=adjust_fn)
rec_dict = gff_iterator.get_all_features(self._test_gff_file)
assert len(rec_dict) == 1
assert rec_dict.keys() == ['1']
assert len(rec_dict.values()[0].features) == 112
assert rec_dict.values()[0].features[0].qualifiers['read_name'] == \
['3_336_815_F3']
def main(seq_file, gff_file):
# -- To be customized
# You need to update these parameters to point to your local database
user = "******"
passwd = "cdev"
host = "localhost"
db_name = "wb199_gff"
biodb_name = "wb199_gff_cds_pcr"
# These need to be updated to reflect what you would like to parse
# out of the GFF file. Set limit_info=None to parse everything, but
# be sure the file is small or you may deal with memory issues.
rnai_types = [('Orfeome', 'PCR_product'),
('GenePair_STS', 'PCR_product'),
('Promoterome', 'PCR_product')]
gene_types = [('Non_coding_transcript', 'gene'),
('Coding_transcript', 'gene'),
('Coding_transcript', 'mRNA'),
('Coding_transcript', 'CDS')]
limit_info = dict(gff_source_type = rnai_types + gene_types)
# --
print "Parsing FASTA sequence file..."
with open(seq_file) as seq_handle:
seq_dict = SeqIO.to_dict(SeqIO.parse(seq_handle, "fasta"))
print "Parsing GFF data file..."
feature_adder = GFFAddingIterator(seq_dict)
rec_dict = feature_adder.get_all_features(gff_file, limit_info)
recs = rec_dict.values()
print "Writing to BioSQL database..."
server = BioSeqDatabase.open_database(driver="MySQLdb", user=user,
passwd=passwd, host=host, db=db_name)
try:
if biodb_name not in server.keys():
server.new_database(biodb_name)
else:
server.remove_database(biodb_name)
server.adaptor.commit()
server.new_database(biodb_name)
db = server[biodb_name]
db.load(recs)
server.adaptor.commit()
except:
server.adaptor.rollback()
raise
def t_gff3_iterator_limit(self):
"""Iterated interface using a limit query on GFF3 files.
"""
gff_iterator = GFFAddingIterator()
cds_limit_info = dict(
gff_source_type = [('Coding_transcript', 'gene'),
('Coding_transcript', 'mRNA'),
('Coding_transcript', 'CDS')],
gff_id = ['I']
)
it_recs = []
for rec_dict in gff_iterator.get_features(self._test_gff_file,
limit_info=cds_limit_info):
it_recs.append(rec_dict)
assert len(it_recs) == 1
tfeature = it_recs[0]["I"].features[0].sub_features[0]
for sub_test in tfeature.sub_features:
assert sub_test.type == "CDS", sub_test
def t_gff3_to_gff3(self):
"""Read in and write out GFF3 without any loss of information.
"""
gff_iterator = GFFAddingIterator()
recs = gff_iterator.get_all_features(self._test_gff_file)
out_handle = StringIO.StringIO()
writer = GFF3Writer()
writer.write(recs.values(), out_handle)
wrote_handle = StringIO.StringIO(out_handle.getvalue())
(_, tmp_file) = tempfile.mkstemp(dir=self._test_dir)
try:
tmp_handle = open(tmp_file, "w")
tmp_handle.write(wrote_handle.read())
tmp_handle.close()
recs_two = gff_iterator.get_all_features(tmp_file)
finally:
os.remove(tmp_file)
orig_rec = recs.values()[0]
re_rec = recs.values()[0]
assert len(orig_rec.features) == len(re_rec.features)
for i, orig_f in enumerate(orig_rec.features):
assert str(orig_f) == str(re_rec.features[i])
