def compute_mutagenesis_primers(self):
"""
Using the sequence fragments to be assembled together, computes for
each step the primers that are to be used to assemble the mutated
fragments.
"""
for step, frags in self.fragments.items():
# Run the standard PrimerDesigner protocol.
p = PrimerDesigner()
p.set_sequences(frags)
# Ensure to set the filename attribute.
p.filename = 'step{step}'.format(step=step)
p.construct_graph()
p.compute_assembly_primers()
p.compute_junction_sequencing_primers()
p.compute_pcr_protocol()
self.primer_designers[step] = p
