def _console_main():
mode = _mode_select()
forward = _primer_select('f')
reverse = _primer_select('r')
Tm = _Tm_select()
tag = _tag_select(mode)
if tag != 'None':
tagfr = _tag_fr_select()
else:
tagfr = 'f'
if tagfr == 'f':
ftag = tag
rtag = 'None'
else:
rtag = tag
ftag = 'None'
print('Processing data...')
data = fasta_reader(mode)
processed = []
for item in data:
processed.append(primer_builder('f', item, forward, ftag, Tm, mode))
processed.append(primer_builder('r', item, reverse, rtag, Tm, mode))
fasta_writer(processed, 99999999, False)
print('Task finished.')
input('Press any key to exit.')
return
def buildNOW(self):
mode = self.buttonGroup_2.checkedButton().text()
forward = self._primer_select('f', self.text_re1.toPlainText())
reverse = self._primer_select('r', self.text_re2.toPlainText())
r_data = self._dna_select(self.text_DNA.toPlainText(), mode)
r_tm = self._Tm_select(self.text_tm.text())
r_tag = self._tag_select(mode)
for i in [forward,reverse,r_data,r_tm,r_tag]:
if i == '':
self.result_err_text += 'Please fill in the information. \n'
break
if self.tag_no.isChecked():
if self.sense_yes.isChecked() == False and self.sense_anti.isChecked() == False:
self.result_err_text += 'Please check sense or antisense'
if i == 'err':
self.result_err_text += 'Error: Forward or backward primer does not exist in dictionary "enzset". \n'
else:
if self.sense_yes.isChecked():
ftag = r_tag
if self.sense_anti.isChecked():
rtag = r_tag
processed = []
for item in r_data:
processed.append(primer_builder('f', item, forward, ftag, r_tm, mode))
processed.append(primer_builder('r', item, reverse, rtag, r_tm, mode))
fasta_writer(processed, 999999999, False)
print('Task finished.')
f_ = open("./output.txt", 'r')
with f_:
text = f_.read()
self.result_text.setText(text)