def get_voltage(self, neuron_config, stim_name):
ephys_sweeps = self.cfg.ephys_sweeps
ephys_sweep = next(s for s in ephys_sweeps
if s['stimulus_name'] == stim_name)
ds = NwbDataSet(self.ephys_file_name)
data = ds.get_sweep(ephys_sweep['sweep_number'])
stimulus = data['stimulus']
stimulus = stimulus[stimulus != 0]
stimulus = stimulus[:self.cfg.stimulus_allow]
# initialize the neuron
neuron = GlifNeuron.from_dict(neuron_config)
# Set dt
neuron.dt = 1.0 / data['sampling_rate']
# simulate the neuron
output = neuron.run(stimulus)
voltage = output['voltage'] * 1e3
voltage = voltage[~np.isnan(voltage)]
voltage = voltage[:self.cfg.signal_allow]
return output, voltage, neuron, stimulus
def test_spike_times(self):
expected = [
2.937305, 3.16453 , 3.24271 , 4.1622 , 4.24182 ,
10.0898 , 10.132545, 10.176095, 10.2361 , 10.660655,
10.762125, 10.863465, 10.93833 , 11.140815, 11.19246 ,
11.24553 , 11.696305, 11.812655, 11.90469 , 12.056755,
12.15794 , 12.233905, 12.47577 , 12.741295, 12.82861 ,
12.923175, 18.05068 , 18.139875, 18.17693 , 18.221485,
18.24337 , 18.39981 , 18.470705, 18.759675, 18.82183 ,
18.877475, 18.91033 , 18.941195, 19.050515, 19.12557 ,
19.15963 , 19.188655, 19.226205, 19.29813 , 19.420665,
19.47627 , 19.763365, 19.824225, 19.897995, 19.93155 ,
20.04916 , 20.11832 , 20.148755, 20.18004 , 20.22173 ,
20.2433 , 20.40018 , 20.470915, 20.759715, 20.82156 ,
20.866465, 20.90807 , 20.939175]
bp = BiophysicalPerisomaticApi('http://api.brain-map.org')
bp.cache_stimulus = True # change to False to not download the large stimulus NWB file
neuronal_model_id = 472451419 # get this from the web site as above
bp.cache_data(neuronal_model_id, working_directory='neuronal_model')
cwd = os.path.realpath(os.curdir)
print(cwd)
os.chdir(os.path.join(cwd, 'neuronal_model'))
manifest = ju.read('manifest.json')
manifest['biophys'][0]['model_file'][0] = 'manifest_51.json'
manifest['runs'][0]['sweeps'] = [51]
ju.write('manifest_51.json', manifest)
subprocess.call(['nrnivmodl', './modfiles'])
run(Config().load('manifest_51.json'))
#os.chdir(cwd)
nwb_out = NwbDataSet('work/386049444.nwb')
spikes = nwb_out.get_spike_times(51)
numpy.testing.assert_array_almost_equal(spikes, expected)
def calc_ev(ew, folder, s, sweeps, stim_len, data_spike_times, dt):
'''
'''
print ew, folder
#convert data times to indicies
data_spike_ind = []
for d in data_spike_times:
data_spike_ind.append((d / dt).astype(int))
#get model data
path = get_model_nwb_path_from_folder(ew, folder, s) #get nwb file path
if isinstance(path, basestring):
model = NwbDataSet(path)
model_spike_ind = []
for sw in sweeps:
spikes = (model.get_spike_times(sw) / dt).astype(int)
model_spike_ind.append(spikes)
#check to make sure all spike time arrays are the same for the model
for ii in range(1, len(model_spike_ind)):
if not np.array_equal(model_spike_ind[ii],
model_spike_ind[ii - 1]):
print 'MODEL SPIKE TIMES SHOULD BE THE SAME AND THEY ARE NOT!', os.path.basename(
folder)[:9]
print len(model_spike_ind), model_spike_ind
# raise Exception('model spike times should be the same and they are not')
return exVar(data_spike_ind, [model_spike_ind[0]], sigma, dt, stim_len)
else:
return np.nan
def set_up_objective(self, measure='spike frequency'):
'''
Prepares the model for parameter optimization by assigning the output measure to be used in the cost function.
Parameters
----------
measure: string
Name of the output measure to be used in optimization. Currently only 'spike frequency' is implemented.
'''
if (measure == 'spike frequency'):
# get the experimental data from the NWB file
data_set = NwbDataSet(
os.path.join(
self.model_dir,
self.description.manifest.get_path('stimulus_path')))
spike_times = data_set.get_spike_times(self.reference_sweep)
# calculate firing frequency for the NWB data
sum_intervals = 0.0
for i in range(len(spike_times) - 1):
sum_intervals += (spike_times[i + 1] - spike_times[i])
self.reference_output = len(spike_times) / sum_intervals
else:
print "Model fitting using the output measure", measure, "has not been implemented yet."
def read_stimulus(self, stimulus_path, sweep=0):
'''Load current values for a specific experiment sweep and setup simulation
and stimulus sampling rates.
NOTE: NEURON only allows simulation timestamps of multiples of 40KHz. To
avoid aliasing, we set the simulation sampling rate to the least common
multiple of the stimulus sampling rate and 40KHz.
Parameters
----------
stimulus path : string
NWB file name
sweep : integer, optional
sweep index
'''
Utils._log.info("reading stimulus path: %s, sweep %s", stimulus_path,
sweep)
stimulus_data = NwbDataSet(stimulus_path)
sweep_data = stimulus_data.get_sweep(sweep)
# convert to nA for NEURON
self.stim_curr = sweep_data['stimulus'] * 1.0e9
# convert from Hz
hz = int(sweep_data['sampling_rate'])
neuron_hz = Utils.nearest_neuron_sampling_rate(hz)
self.simulation_sampling_rate = neuron_hz
self.stimulus_sampling_rate = hz
if hz != neuron_hz:
Utils._log.debug(
"changing sampling rate from %d to %d to avoid NEURON aliasing",
hz, neuron_hz)
def save_nwb(output_path, v, sweep, sweeps_by_type):
'''Save a single voltage output result into an existing sweep in a NWB file.
This is intended to overwrite a recorded trace with a simulated voltage.
Parameters
----------
output_path : string
file name of a pre-existing NWB file.
v : numpy array
voltage
sweep : integer
which entry to overwrite in the file.
'''
output = NwbDataSet(output_path)
output.set_sweep(sweep, None, v)
sweep_by_type = {t: [ sweep ] for t, ss in sweeps_by_type.items() if sweep in ss }
sweep_features = extract_cell_features.extract_sweep_features(output,
sweep_by_type)
try:
spikes = sweep_features[sweep]['spikes']
spike_times = [ s['threshold_t'] for s in spikes ]
output.set_spike_times(sweep, spike_times)
except Exception, e:
logging.info("sweep %d has no sweep features. %s" % (sweep, e.message) )
def read_stimulus(self, stimulus_path, sweep=0):
'''Load current values for a specific experiment sweep and setup simulation
and stimulus sampling rates.
NOTE: NEURON only allows simulation timestamps of multiples of 40KHz. To
avoid aliasing, we set the simulation sampling rate to the least common
multiple of the stimulus sampling rate and 40KHz.
Parameters
----------
stimulus path : string
NWB file name
sweep : integer, optional
sweep index
'''
Utils._log.info(
"reading stimulus path: %s, sweep %s",
stimulus_path,
sweep)
stimulus_data = NwbDataSet(stimulus_path)
sweep_data = stimulus_data.get_sweep(sweep)
# convert to nA for NEURON
self.stim_curr = sweep_data['stimulus'] * 1.0e9
# convert from Hz
hz = int(sweep_data['sampling_rate'])
neuron_hz = Utils.nearest_neuron_sampling_rate(hz)
self.simulation_sampling_rate = neuron_hz
self.stimulus_sampling_rate = hz
if hz != neuron_hz:
Utils._log.debug("changing sampling rate from %d to %d to avoid NEURON aliasing", hz, neuron_hz)
def ve_tau(specimen_id, ve_path):
#print(chr(27) + "[2J") # To clear terminal screen
print "START VE_TAU " + str(specimen_id) + " " + str(ve_path)
expt_taus = []
data_set = NwbDataSet(ve_path)
long_square_sweeps = lims_utils.get_sweeps_of_type("C1LSCOARSE",
specimen_id,
passed_only=True)
print "ve specimen id= " + str(specimen_id)
for sweep in long_square_sweeps:
#print "ve_sweep_number: " + str(sweep)
#print(data_set.get_sweep_metadata(sweep))
try:
(data_set.get_sweep_metadata(sweep)["aibs_stimulus_amplitude_pa"])
except:
continue
else:
if (data_set.get_sweep_metadata(sweep)
["aibs_stimulus_amplitude_pa"] < 0):
v, i, t = lims_utils.get_sweep_v_i_t_from_set(data_set, sweep)
sweep_feat = EphysSweepFeatureExtractor(
t, v) # Get time and voltage of each hyperpolarizing sweep
if np.isnan(sweep_feat):
continue
else:
expt_taus.append(sweep_feat.estimate_time_constant(
)) # Append time constant of each sweep to list
mean_expt_tau = np.nanmean(expt_taus) # Mean time constant for this cell
print "mean_ve_tau= " + str(mean_expt_tau)
return mean_expt_tau
def ve_ramp_latency(specimen_id, ve_path):
data_set = NwbDataSet(ve_path)
ramp_sweeps = lims_utils.get_sweeps_of_type("C1RP25PR1S", specimen_id, passed_only=True)
if len(ramp_sweeps) == 0:
return np.nan
spike_times = data_set.get_spike_times(ramp_sweeps[0])
if len(spike_times) > 0:
return spike_times[0]
else:
return np.nan
class Nwb1Appender(NwbAppender):
def __init__(self, nwb_file_name):
NwbAppender.__init__(self, nwb_file_name)
self.nwbfile = NwbDataSet(self.nwb_file_name)
def add_spike_times(self, sweep_spike_times):
for sweep_num, spike_times in sweep_spike_times.items():
self.nwbfile.set_spike_times(sweep_num, spike_times)
def load_sweep(file_name, sweep_number, desired_dt=None, cut=0, bessel=False):
'''load a data sweep and do specified data processing.
Inputs:
file_name: string
name of .nwb data file
sweep_number:
number specifying the sweep to be loaded
desired_dt:
the size of the time step the data should be subsampled to
cut:
indicie of which to start reporting data (i.e. cut off data before this indicie)
bessel: dictionary
contains parameters 'N' and 'Wn' to implement standard python bessel filtering
Returns:
dictionary containing
voltage: array
current: array
dt: time step of the returned data
start_idx: the index at which the first stimulus starts (excluding the test pulse)
'''
ds = NwbDataSet(file_name)
data = ds.get_sweep(sweep_number)
data["dt"] = 1.0 / data["sampling_rate"]
if cut > 0:
data["response"] = data["response"][cut:]
data["stimulus"] = data["stimulus"][cut:]
if bessel:
sample_freq = 1. / data["dt"]
filt_coeff = (bessel["freq"]) / (
sample_freq / 2.) # filter fraction of Nyquist frequency
b, a = signal.bessel(bessel["N"], filt_coeff, "low")
data['response'] = signal.filtfilt(b, a, data['response'], axis=0)
if desired_dt is not None:
if data["dt"] != desired_dt:
data["response"] = subsample_data(data["response"], "mean",
data["dt"], desired_dt)
data["stimulus"] = subsample_data(data["stimulus"], "mean",
data["dt"], desired_dt)
data["start_idx"] = int(data["index_range"][0] /
(desired_dt / data["dt"]))
data["dt"] = desired_dt
if "start_idx" not in data:
data["start_idx"] = data["index_range"][0]
return {
"voltage": data["response"],
"current": data["stimulus"],
"dt": data["dt"],
"start_idx": data["start_idx"]
}
def stimulus(neuron_config_file, ephys_sweeps_file):
ephys_sweeps = json_utilities.read(ephys_sweeps_file)
ephys_file_name = 'stimulus.nwb'
# pull out the stimulus for the first sweep
ephys_sweep = ephys_sweeps[0]
ds = NwbDataSet(ephys_file_name)
data = ds.get_sweep(ephys_sweep['sweep_number'])
stimulus = data['stimulus']
return stimulus
def load_experiment(file_name, sweep_number):
ds = NwbDataSet(file_name)
sweep = ds.get_sweep(sweep_number)
r = sweep['index_range']
v = sweep['response'] * 1e3
i = sweep['stimulus'] * 1e12
dt = 1.0 / sweep['sampling_rate']
t = np.arange(0, len(v)) * dt
return (v, i, t, r, dt)
def write_sweep_response(file_name, sweep_number, response, spike_times):
''' Overwrite the response in a file. '''
logging.debug("writing sweep")
write_start_time = time.time()
ephds = NwbDataSet(file_name)
ephds.set_sweep(sweep_number, stimulus=None, response=response)
ephds.set_spike_times(sweep_number, spike_times)
logging.debug("write time %f" % (time.time() - write_start_time))
def stimulus(neuron_config_file, ephys_sweeps_file):
neuron_config = json_utilities.read(neuron_config_file)
ephys_sweeps = json_utilities.read(ephys_sweeps_file)
ephys_file_name = 'stimulus.nwb'
# pull out the stimulus for the first sweep
ephys_sweep = ephys_sweeps[0]
ds = NwbDataSet(ephys_file_name)
data = ds.get_sweep(ephys_sweep['sweep_number'])
stimulus = data['stimulus']
return stimulus
def from_electrophysiology(
cell_id: int,
ephys: NwbDataSet,
duration=2.0) -> 'ProcessedAllenNeuronElectrophysiology':
current_list = []
voltage_list = []
time_list = []
stim_amp_list = []
n_spikes_list = []
spike_features_list = []
for sweep_number in ephys.get_sweep_numbers():
sweep_metadata = ephys.get_sweep_metadata(sweep_number)
if sweep_metadata['aibs_stimulus_name'] == 'Long Square':
sweep_data = ephys.get_sweep(sweep_number)
amp = sweep_metadata['aibs_stimulus_amplitude_pa']
index_range = sweep_data["index_range"]
sampling_rate = sweep_data["sampling_rate"]
current = sweep_data["stimulus"][
index_range[0]:index_range[1] + 1]
voltage = sweep_data["response"][
index_range[0]:index_range[1] + 1]
# truncate
max_frames = int(duration * sampling_rate)
assert max_frames < len(voltage)
current = current[:max_frames] * 1e12 # in pA
voltage = voltage[:max_frames] * 1e3 # in mV
# extract featrures
time = np.arange(0, max_frames,
dtype=np.float) / sampling_rate # in seconds
ext = EphysSweepFeatureExtractor(t=time, v=voltage, i=current)
ext.process_spikes()
spike_features = ext.spikes()
n_spikes = len(spike_features)
current_list.append(current)
voltage_list.append(voltage)
time_list.append(time)
stim_amp_list.append(amp)
n_spikes_list.append(n_spikes)
spike_features_list.append(spike_features)
return ProcessedAllenNeuronElectrophysiology(
cell_id=cell_id,
current_list=current_list,
voltage_list=voltage_list,
time_list=time_list,
stim_amp_list=stim_amp_list,
n_spikes_list=n_spikes_list,
spike_features_list=spike_features_list)
def get_sweep_from_nwb(nwb_file, sweep_num):
'''
Read a sweep from an NWB file and convert Volts -> mV and Amps -> pA.
'''
ds = NwbDataSet(nwb_file)
data = ds.get_sweep(sweep_num)
v = data['response'] * 1e3 # convert to mV
i = data['stimulus'] * 1e12 # convert to pA
dt = 1.0 / data['sampling_rate']
t = np.arange(0,len(v)) * dt
return (v, i, t)
def read_stimulus(self, stimulus_path, sweep=0):
"""load current values for a specific experiment sweep.
Parameters
----------
stimulus path : string
NWB file name
sweep : integer, optional
sweep index
"""
Utils._log.info("reading stimulus path: %s, sweep %s" % (stimulus_path, sweep))
stimulus_data = NwbDataSet(stimulus_path)
sweep_data = stimulus_data.get_sweep(sweep)
self.stim_curr = sweep_data["stimulus"] * 1.0e9 # convert to nA for NEURON
self.sampling_rate = 1.0e3 / sweep_data["sampling_rate"] # convert from Hz
def save_nwb(output_path, v, sweep):
"""Save a single voltage output result into an existing sweep in a NWB file.
This is intended to overwrite a recorded trace with a simulated voltage.
Parameters
----------
output_path : string
file name of a pre-existing NWB file.
v : numpy array
voltage
sweep : integer
which entry to overwrite in the file.
"""
output = NwbDataSet(output_path)
output.set_sweep(sweep, None, v)
def get_ephys_data(self, specimen_id, file_name=None):
"""
Download electrophysiology traces for a single cell in the database.
Parameters
----------
specimen_id: int
The ID of a cell specimen to download.
file_name: string
File name to save/read the ephys features metadata as CSV.
If file_name is None, the file_name will be pulled out of the
manifest. If caching is disabled, no file will be saved.
Default is None.
Returns
-------
NwbDataSet
A class instance with helper methods for retrieving stimulus
and response traces out of an NWB file.
"""
file_name = self.get_cache_path(file_name, self.EPHYS_DATA_KEY, specimen_id)
if not os.path.exists(file_name):
self.api.save_ephys_data(specimen_id, file_name)
return NwbDataSet(file_name)
def get_model_spike_times_from_nwb(ends_with, specimen_id_directory,
model_string, sweeps, where_running):
''' Gets the times of spike from the model nwb file
inputs
ends_with: string
end of file searching for: options "_GLIF1_neuron_config.json","_GLIF2_neuron_config.json' etc."
specimen_id_directory: string
path to structured data directory containing neuron_config, preprocessor, etc., files.
model_string: string
string searching for in model name: options '(LIF)', '(LIF-R)', '(LIF-ASC)', '(LIF-R_ASC)', '(LIF-R_ASC_A')
sweeps: list of integers
integers refer to the sweep number in the electrophysiology .nwb data file
where_running: string
options are 'internal': the code is being run within the Institute and can therefore access the internal file system
'external': the code is being run outside the Institute and requires the use of the api to download the model nwb files
Note that although ends_with and model_string should be appropriately paired, there is no check
within this module to make sure that they are
outputs: returns either a
nan if the there is not a model in the structured data directory corresponding to what the requested ends_with variable
or
model_spike_times: list of numpy arrays
each array contains the times of the spikes in each sweep
'''
if where_running == 'internal':
path = get_model_nwb_path_from_folder(ends_with, specimen_id_directory,
model_string) #get nwb file path
elif where_running == 'external':
path = download_model_nwb_if_model_exists_in_SDD(
ends_with, specimen_id_directory, model_string) #get nwb file path
else:
raise Exception(
'specify whether the code is being run internally or externally')
if isinstance(path, basestring):
model = NwbDataSet(path)
model_spike_times = []
if sweeps == []:
raise Exception('There are no sweeps to look at')
for sw in sweeps:
model_spike_times.append(model.get_spike_times(sw))
return model_spike_times
else:
return np.nan
def load_sweep(file_name, sweep_number):
''' Load the stimulus for a sweep from file. '''
logging.debug("loading sweep %d" % sweep_number)
load_start_time = time.time()
data = NwbDataSet(file_name).get_sweep(sweep_number)
logging.debug("load time %f" % (time.time() - load_start_time))
return data
def get_sweep_data(nwb_file, sweep_number, time_scale=1e3, voltage_scale=1e3, stim_scale=1e12):
"""
Extract data and stim characteristics for a specific DC sweep from nwb file
Parameters
----------
nwb_file : string
File name of a pre-existing NWB file.
sweep_number : integer
time_scale : float
Convert to ms scale
voltage_scale : float
Convert to mV scale
stim_scale : float
Convert to pA scale
Returns
-------
t : numpy array
Sampled time points in ms
v : numpy array
Recorded voltage at the sampled time points in mV
stim_start_time : float
Stimulus start time in ms
stim_end_time : float
Stimulus end time in ms
"""
nwb = NwbDataSet(nwb_file)
sweep = nwb.get_sweep(sweep_number)
stim = sweep['stimulus'] * stim_scale # in pA
stim_diff = np.diff(stim)
stim_start = np.where(stim_diff != 0)[0][-2]
stim_end = np.where(stim_diff != 0)[0][-1]
# read v and t as numpy arrays
v = sweep['response'] * voltage_scale # in mV
dt = time_scale / sweep['sampling_rate'] # in ms
num_samples = len(v)
t = np.arange(num_samples) * dt
stim_start_time = t[stim_start]
stim_end_time = t[stim_end]
return t, v, stim_start_time, stim_end_time
def expt_data_set(specimen_id):
sql = """
select wkf.storage_directory || wkf.filename from well_known_files wkf
join specimens sp on sp.ephys_roi_result_id = wkf.attachable_id
where sp.id = %s
and wkf.well_known_file_type_id = %s
"""
results = lims_utils.query(sql, (specimen_id, NWB_DOWNLOAD_TYPE_ID))
nwb_path = results[0][0]
return NwbDataSet(nwb_path)
def ve_fi_curve(specimen_id, ve_path):
data_set = NwbDataSet(ve_path)
expt_set = expt_data_set(specimen_id)
long_square_sweeps = lims_utils.get_sweeps_of_type("C1LSCOARSE",
specimen_id,
passed_only=True)
fi_curve_data = dict([
amp_and_spike_count(data_set, sweep, expt_set)
for sweep in long_square_sweeps
])
return fi_curve_stats(fi_curve_data)
def extract_single_sweep_features(features, nwb_file, sweep_number):
'''
Run feature extraction on a single sweep.
Parameters
----------
features: EphysFeatureExtractor instance
nwb_file: string
File name of an NWB file
sweep_numbers: int
Sweep number in the NWB file
'''
nwb = NwbDataSet(nwb_file)
data = nwb.get_sweep(sweep_number)
v = data['response']
curr = data['stimulus']
idx0 = data['index_range'][0]
idx1 = data['index_range'][1]
if idx0 >= idx1:
logging.warning("Sweep %s stop index precedes start index, skipping spike identification" % sweep_number)
return
hz = data['sampling_rate']
dt = 1.0 / hz
t = np.arange(0, len(v)) * dt
features.process_instance(sweep_number, v*1e3, curr*1e12, t, dt*idx0, dt*(idx1-idx0-2), None)
results = {}
results["mean"] = features.feature_list[-1].mean
results["stdev"] = features.feature_list[-1].stdev
return results
def output():
neuron_config = json_utilities.read('neuron_config.json')
ephys_sweeps = json_utilities.read('ephys_sweeps.json')
ephys_file_name = 'stimulus.nwb'
# pull out the stimulus for the first sweep
ephys_sweep = ephys_sweeps[0]
ds = NwbDataSet(ephys_file_name)
data = ds.get_sweep(ephys_sweep['sweep_number'])
stimulus = data['stimulus']
# initialize the neuron
# important! update the neuron's dt for your stimulus
neuron = GlifNeuron.from_dict(neuron_config)
neuron.dt = 1.0 / data['sampling_rate']
# simulate the neuron
truncate = 56041
output = neuron.run(stimulus[0:truncate])
return output
def save_nwb(output_path, v, sweep):
'''Save a single voltage output result into an existing sweep in a NWB file.
This is intended to overwrite a recorded trace with a simulated voltage.
Parameters
----------
output_path : string
file name of a pre-existing NWB file.
v : numpy array
voltage
sweep : integer
which entry to overwrite in the file.
'''
output = NwbDataSet(output_path)
output.set_sweep(sweep, None, v)
sweep_features = extract_cell_features.extract_sweep_features(output_path,
[sweep])
spikes = sweep_features[sweep]['mean']['spikes']
spike_times = [ s['t'] for s in spikes ]
output.set_spike_times(sweep, spike_times)
def output(neuron_config_file, ephys_sweeps_file):
neuron_config = json_utilities.read(neuron_config_file)
ephys_sweeps = json_utilities.read(ephys_sweeps_file)
ephys_file_name = 'stimulus.nwb'
# pull out the stimulus for the first sweep
ephys_sweep = ephys_sweeps[0]
ds = NwbDataSet(ephys_file_name)
data = ds.get_sweep(ephys_sweep['sweep_number'])
stimulus = data['stimulus']
# initialize the neuron
# important! update the neuron's dt for your stimulus
neuron = GlifNeuron.from_dict(neuron_config)
neuron.dt = 1.0 / data['sampling_rate']
# simulate the neuron
truncate = 56041
output = neuron.run(stimulus[0:truncate])
return output
def get_sweep_data(sweep_name):
""" Input: sweep name (string)
Output: NwbDataSet object
"""
global nwb_file_name
try:
num = int(sweep_name.split('_')[-1])
except:
print("Unable to parse sweep number from '%s'" % str(sweep_name))
raise
return NwbDataSet(nwb_file_name).get_sweep(num)
def read_stimulus(self, stimulus_path, sweep=0):
'''load current values for a specific experiment sweep.
Parameters
----------
stimulus path : string
NWB file name
sweep : integer, optional
sweep index
'''
Utils._log.info("reading stimulus path: %s, sweep %s", stimulus_path,
sweep)
stimulus_data = NwbDataSet(stimulus_path)
sweep_data = stimulus_data.get_sweep(sweep)
# convert to nA for NEURON
self.stim_curr = sweep_data['stimulus'] * 1.0e9
# convert from Hz
self.sampling_rate = 1.0e3 / sweep_data['sampling_rate']
def save_nwb(output_path, v, sweep, sweep_by_type= None):
'''Save a single voltage output result into an existing sweep in a NWB file.
This is intended to overwrite a recorded trace with a simulated voltage.
Parameters
----------
output_path : string
file name of a pre-existing NWB file.
v : numpy array
voltage
sweep : integer
which entry to overwrite in the file.
'''
output = NwbDataSet(output_path)
output.set_sweep(sweep, None, v)
if sweep_by_type is not None:
sweep_by_type = {t: [sweep]
for t, ss in sweeps_by_type.items() if sweep in ss}
sweep_features = extract_cell_features.extract_sweep_features(output,
sweep_by_type)
try:
spikes = sweep_features[sweep]['spikes']
spike_times = [s['threshold_t'] for s in spikes]
output.set_spike_times(sweep, spike_times)
except Exception as e:
logging.info("sweep %d has no sweep features. %s" % (sweep, e.args))
def get_data_sets_from_remote(upper_bound=2, lower_bound=None):
try:
with open('all_allen_cells.p', 'rb') as f:
cells = pickle.load(f)
ctc = CellTypesCache(manifest_file='cell_types/manifest.json')
except:
ctc = CellTypesCache(manifest_file='cell_types/manifest.json')
cells = ctc.get_cells()
with open('all_allen_cells.p', 'wb') as f:
pickle.dump(cells, f)
data = []
data_sets = []
path_name = 'data_nwbs'
try:
os.mkdir(path_name)
except:
print('directory already made.')
ids = [c['id'] for c in cells]
if upper_bound == None and lower_bound is None:
limited_range = ids[0:-1]
elif upper_bound is not None and lower_bound is not None:
limited_range = ids[lower_bound:upper_bound]
cnt = 0
for specimen_id in limited_range:
temp_path = str(path_name) + str('/') + str(specimen_id) + '.p'
if os.path.exists(temp_path):
cnt += 1
for specimen_id in limited_range:
temp_path = str(path_name) + str('/') + str(specimen_id) + '.p'
if os.path.exists(temp_path):
with open(temp_path, 'rb') as f:
(data_set_nwb, sweeps, specimen_id) = pickle.load(f)
data_sets.append((data_set_nwb, sweeps, specimen_id))
else:
data_set = ctc.get_ephys_data(specimen_id)
sweeps = ctc.get_ephys_sweeps(specimen_id)
file_name = 'cell_types/specimen_' + str(
specimen_id) + '/ephys.nwb'
data_set_nwb = NwbDataSet(file_name)
data_sets.append((data_set_nwb, sweeps, specimen_id))
with open(temp_path, 'wb') as f:
pickle.dump((data_set_nwb, sweeps, specimen_id), f)
return data_sets
def embed_spike_times(input_nwb_file, output_nwb_file, sweep_features):
# embed spike times in NWB file
tmp_nwb_file = output_nwb_file + ".tmp"
shutil.copy(input_nwb_file, tmp_nwb_file)
for sweep_num in sweep_features:
spikes = sweep_features[sweep_num]['spikes']
spike_times = [ s['threshold_t'] for s in spikes ]
NwbDataSet(tmp_nwb_file).set_spike_times(sweep_num, spike_times)
try:
shutil.move(tmp_nwb_file, output_nwb_file)
except OSError as e:
logging.error("Problem renaming file: %s -> %s" % (tmp_nwb_file, output_nwb_file))
raise e
logging.debug("Embedded spike times into output.nwb file")
def load_experiment(specimen):
path, bath, breakin, end, giga, last_bias, mid_bias, SS_amp, spec_name = Find_Critical_Sweeps(
specimen)
ds = NwbDataSet(path)
if bath is 'error':
bath_peak = 9999999
bath_ss = 9999999
else:
bath_sweep = ds.get_sweep(bath)
bath_i = bath_sweep['response'] * 1e12
bath_peak, bath_ss = get_Reses(70, bath_i)
if breakin is 'error':
breakin_peak = 9999999
breakin_ss = 9999999
else:
breakin_sweep = ds.get_sweep(breakin)
breakin_i = breakin_sweep['response'] * 1e12
breakin_peak, breakin_ss = get_Reses(70, breakin_i)
if end is 'error':
end_peak = 9999999
end_ss = 9999999
end_leak = 9999999
else:
end_sweep = ds.get_sweep(end)
end_i = end_sweep['response'] * 1e12
end_peak, end_ss = get_Reses(70, end_i)
end_leak = np.mean(end_i[0:100])
if giga is 'error':
giga_peak = 9999999
giga_ss = 9999999
else:
giga_sweep = ds.get_sweep(giga)
giga_i = giga_sweep['response'] * 1e12
giga_peak, giga_ss = get_Reses(70, giga_i)
features = []
features.append(spec_name)
features.append(bath_ss)
features.append(breakin_peak)
features.append(breakin_ss)
features.append(end_peak)
features.append(end_ss)
features.append(end_leak)
features.append(giga_ss)
features.append(last_bias)
features.append(mid_bias)
features.append(SS_amp)
return features
def main():
"""Main sequence of pre-processing and passive fitting"""
# This argschema package reads arguments from a JSON file
module = ags.ArgSchemaParser(schema_type=PreprocessorParameters,
logger_name=None)
nwb_path = module.args["paths"][
"nwb"] # nwb - neurodata without borders (ephys data)
swc_path = module.args["paths"]["swc"] # swc - morphology data
storage_directory = module.args["paths"]["storage_directory"]
try:
paths, results, passive_info, s1_tasks, s2_tasks = \
preprocess(data_set=NwbDataSet(nwb_path),
swc_data=pd.read_table(swc_path, sep='\s+', comment='#', header=None),
dendrite_type_tag=module.args["dendrite_type_tag"],
sweeps=module.args["sweeps"],
bridge_avg=module.args["bridge_avg"],
storage_directory=storage_directory)
except NoUsableSweepsException as e:
ju.write(module.args["output_json"], {'error': e.message})
return
preprocess_results_path = os.path.join(storage_directory,
"preprocess_results.json")
ju.write(preprocess_results_path, results)
passive_info_path = os.path.join(storage_directory, "passive_info.json")
ju.write(passive_info_path, passive_info)
paths.update({
"swc": swc_path,
"nwb": nwb_path,
"storage_directory": storage_directory,
"preprocess_results": preprocess_results_path,
"passive_info": passive_info_path,
})
output = {
"paths": paths,
"stage_1_task_list": s1_tasks,
"stage_2_task_list": s2_tasks,
}
ju.write(module.args["output_json"], output)
def write_sweep_response(file_name, sweep_number, response, spike_times):
''' Overwrite the response in a file. '''
logging.debug("writing sweep")
write_start_time = time.time()
ephds = NwbDataSet(file_name)
ephds.set_sweep(sweep_number, stimulus=None, response=response)
ephds.set_spike_times(sweep_number, spike_times)
logging.debug("write time %f" % (time.time() - write_start_time))
def prepare_nwb_output(nwb_stimulus_path, nwb_result_path):
"""Copy the stimulus file, zero out the recorded voltages and spike times.
Parameters
----------
nwb_stimulus_path : string
NWB file name
nwb_result_path : string
NWB file name
"""
copy(nwb_stimulus_path, nwb_result_path)
data_set = NwbDataSet(nwb_result_path)
data_set.fill_sweep_responses(0.0)
for sweep in data_set.get_sweep_numbers():
data_set.set_spike_times(sweep, [])
def ve_ap_dim(specimen_id, ve_path):
data_set = NwbDataSet(ve_path)
expt_set = expt_data_set(specimen_id)
long_square_sweeps = lims_utils.get_sweeps_of_type("C1LSCOARSE",
specimen_id,
passed_only=True)
fi_curve_data = dict([
amp_and_spike_count(data_set, sweep, expt_set)
for sweep in long_square_sweeps
])
sweeps_by_amp = {
amp_and_spike_count(data_set, sweep, expt_set)[0]: sweep
for sweep in long_square_sweeps
}
fi_arr = np.array([(amp, fi_curve_data[amp])
for amp in sorted(fi_curve_data.keys())])
spiking_sweeps = np.flatnonzero(fi_arr[:, 1])
if len(spiking_sweeps) == 0:
return np.nan, np.nan
rheo_sweep = sweeps_by_amp[fi_arr[spiking_sweeps[0], 0]]
# print specimen_id, rheo_sweep
v, i, t = lims_utils.get_sweep_v_i_t_from_set(data_set, rheo_sweep)
swp_ext = EphysSweepFeatureExtractor(t,
v,
start=1.02,
end=2.02,
filter=None)
swp_ext.process_spikes()
if len(swp_ext.spike_feature("width")) == 0:
print "NO SPIKES FOR {:d} ON SWEEP {:d}".format(
specimen_id, sweeps_by_amp[fi_arr[spiking_sweeps[0], 0]])
print fi_arr
print sweeps_by_amp
return np.nan, np.nan
return_vals = (swp_ext.spike_feature("width")[0] * 1e3,
swp_ext.spike_feature("peak_v")[0] -
swp_ext.spike_feature("trough_v")[0])
return return_vals
def prepare_nwb_output(nwb_stimulus_path, nwb_result_path):
'''Copy the stimulus file, zero out the recorded voltages and spike times.
Parameters
----------
nwb_stimulus_path : string
NWB file name
nwb_result_path : string
NWB file name
'''
output_dir = os.path.dirname(nwb_result_path)
if not os.path.exists(output_dir):
os.makedirs(output_dir)
copy(nwb_stimulus_path, nwb_result_path)
data_set = NwbDataSet(nwb_result_path)
data_set.fill_sweep_responses(0.0, extend_experiment=True)
for sweep in data_set.get_sweep_numbers():
data_set.set_spike_times(sweep, [])
def prepare_nwb_output(nwb_stimulus_path,
nwb_result_path):
'''Copy the stimulus file, zero out the recorded voltages and spike times.
Parameters
----------
nwb_stimulus_path : string
NWB file name
nwb_result_path : string
NWB file name
'''
output_dir = os.path.dirname(nwb_result_path)
if not os.path.exists(output_dir):
os.makedirs(output_dir)
copy(nwb_stimulus_path, nwb_result_path)
data_set = NwbDataSet(nwb_result_path)
data_set.fill_sweep_responses(0.0)
for sweep in data_set.get_sweep_numbers():
data_set.set_spike_times(sweep, [])
soma = morphology.soma
# all compartments are dictionaries of compartment properties
# compartments also keep track of ids of their children
for child in morphology.children_of(soma):
print(child['x'], child['y'], child['z'], child['radius'])
#===============================================================================
# example 4
#===============================================================================
from allensdk.core.nwb_data_set import NwbDataSet
# if you ran the examples above, you will have a NWB file here
file_name = 'cell_types/specimen_485909730/ephys.nwb'
data_set = NwbDataSet(file_name)
sweep_numbers = data_set.get_sweep_numbers()
sweep_number = sweep_numbers[0]
sweep_data = data_set.get_sweep(sweep_number)
# spike times are in seconds relative to the start of the sweep
spike_times = data_set.get_spike_times(sweep_number)
# stimulus is a numpy array in amps
stimulus = sweep_data['stimulus']
# response is a numpy array in volts
reponse = sweep_data['response']
# sampling rate is in Hz
def prepare_stage_1(description, passive_fit_data):
output_directory = description.manifest.get_path('WORKDIR')
neuronal_model_data = ju.read(description.manifest.get_path('neuronal_model_data'))
specimen_data = neuronal_model_data['specimen']
specimen_id = neuronal_model_data['specimen_id']
is_spiny = not any(t['name'] == u'dendrite type - aspiny' for t in specimen_data['specimen_tags'])
all_sweeps = specimen_data['ephys_sweeps']
data_set = NwbDataSet(description.manifest.get_path('stimulus_path'))
swc_path = description.manifest.get_path('MORPHOLOGY')
if not os.path.exists(output_directory):
os.makedirs(output_directory)
ra = passive_fit_data['ra']
cm1 = passive_fit_data['cm1']
cm2 = passive_fit_data['cm2']
# Check for fi curve shift to decide to use core1 or core2
fi_shift, n_core2 = check_fi_shift.estimate_fi_shift(data_set, all_sweeps)
fi_shift_threshold = 30.0
sweeps_to_fit = []
if abs(fi_shift) > fi_shift_threshold:
_fit_stage_1_log.info("FI curve shifted; using Core 1")
sweeps_to_fit = find_core1_trace(data_set, all_sweeps)
else:
sweeps_to_fit = find_core2_trace(data_set, all_sweeps)
if sweeps_to_fit == []:
_fit_stage_1_log.info("Not enough good Core 2 traces; using Core 1")
sweeps_to_fit = find_core1_trace(data_set, all_sweeps)
_fit_stage_1_log.debug("will use sweeps: " + str(sweeps_to_fit))
jxn = -14.0
t_set = []
v_set = []
i_set = []
for s in sweeps_to_fit:
v, i, t = ephys_utils.get_sweep_v_i_t_from_set(data_set, s)
v += jxn
stim_start, stim_dur, stim_amp, start_idx, end_idx = ephys_utils.get_step_stim_characteristics(i, t)
t_set.append(t)
v_set.append(v)
i_set.append(i)
ext = EphysSweepSetFeatureExtractor(t_set, v_set, i_set, start=stim_start, end=(stim_start + stim_dur))
ext.process_spikes()
ft = {}
blacklist = ["isi_type"]
for k in ext.sweeps()[0].spike_feature_keys():
if k in blacklist:
continue
pair = {}
pair["mean"] = float(ext.spike_feature_averages(k).mean())
pair["stdev"] = float(ext.spike_feature_averages(k).std())
ft[k] = pair
# "Delta" features
sweep_avg_slow_trough_delta_time = []
sweep_avg_slow_trough_delta_v = []
sweep_avg_peak_trough_delta_time = []
for swp in ext.sweeps():
threshold_t = swp.spike_feature("threshold_t")
fast_trough_t = swp.spike_feature("fast_trough_t")
slow_trough_t = swp.spike_feature("slow_trough_t")
delta_t = slow_trough_t - fast_trough_t
delta_t[np.isnan(delta_t)] = 0.
sweep_avg_slow_trough_delta_time.append(np.mean(delta_t[:-1] / np.diff(threshold_t)))
fast_trough_v = swp.spike_feature("fast_trough_v")
slow_trough_v = swp.spike_feature("slow_trough_v")
delta_v = fast_trough_v - slow_trough_v
delta_v[np.isnan(delta_v)] = 0.
sweep_avg_slow_trough_delta_v.append(delta_v.mean())
ft["slow_trough_delta_time"] = {"mean": float(np.mean(sweep_avg_slow_trough_delta_time)),
"stdev": float(np.std(sweep_avg_slow_trough_delta_time))}
ft["slow_trough_delta_v"] = {"mean": float(np.mean(sweep_avg_slow_trough_delta_v)),
"stdev": float(np.std(sweep_avg_slow_trough_delta_v))}
baseline_v = float(ext.sweep_features("v_baseline").mean())
passive_fit_data["e_pas"] = baseline_v
for k in ext.sweeps()[0].sweep_feature_keys():
pair = {}
pair["mean"] = float(ext.sweep_features(k).mean())
pair["stdev"] = float(ext.sweep_features(k).std())
ft[k] = pair
# Determine highest step to check for depolarization block
noise_1_sweeps, _, _ = ephys_utils.get_sweeps_of_type("C1NSSEED_1", all_sweeps)
noise_2_sweeps, _, _ = ephys_utils.get_sweeps_of_type("C1NSSEED_2", all_sweeps)
step_sweeps, _, _ = ephys_utils.get_sweeps_of_type("C1LSCOARSE", all_sweeps)
all_sweeps = noise_1_sweeps + noise_2_sweeps + step_sweeps
max_i = 0
for s in all_sweeps:
try:
v, i, t = ephys_utils.get_sweep_v_i_t_from_set(data_set, s['sweep_number'])
except:
pass
if np.max(i) > max_i:
max_i = np.max(i)
max_i += 10 # add 10 pA
max_i *= 1e-3 # convert to nA
# ----------- Generate output and submit jobs ---------------
# Set up directories
# Decide which fit(s) we are doing
if (is_spiny and ft["width"]["mean"] < 0.8) or (not is_spiny and ft["width"]["mean"] > 0.8):
fit_types = ["f6", "f12"]
elif is_spiny:
fit_types = ["f6"]
else:
fit_types = ["f12"]
for fit_type in fit_types:
fit_type_dir = os.path.join(output_directory, fit_type)
if not os.path.exists(fit_type_dir):
os.makedirs(fit_type_dir)
for seed in SEEDS:
seed_dir = "{:s}/s{:d}".format(fit_type_dir, seed)
if not os.path.exists(seed_dir):
os.makedirs(seed_dir)
# Collect and save data for target.json file
target_dict = {}
target_dict["passive"] = [{
"ra": ra,
"cm": { "soma": cm1, "axon": cm1, "dend": cm2 },
"e_pas": baseline_v
}]
swc_data = pd.read_table(swc_path, sep='\s', comment='#', header=None)
has_apic = False
if APICAL_DENDRITE_TYPE in pd.unique(swc_data[1]):
has_apic = True
_fit_stage_1_log.info("Has apical dendrite")
else:
_fit_stage_1_log.info("Does not have apical dendrite")
if has_apic:
target_dict["passive"][0]["cm"]["apic"] = cm2
target_dict["fitting"] = [{
"junction_potential": jxn,
"sweeps": sweeps_to_fit,
"passive_fit_info": passive_fit_data,
"max_stim_test_na": max_i,
}]
target_dict["stimulus"] = [{
"amplitude": 1e-3 * stim_amp,
"delay": 1000.0,
"duration": 1e3 * stim_dur
}]
target_dict["manifest"] = []
target_dict["manifest"].append({"type": "file", "spec": swc_path, "key": "MORPHOLOGY"})
target_dict["target_features"] = collect_target_features(ft)
target_file = os.path.join(output_directory, 'target.json')
ju.write(target_file, target_dict)
# Create config.json for each fit type
config_base_data = ju.read(os.path.join(FIT_BASE_DIR,
'config_base.json'))
jobs = []
for fit_type in fit_types:
config = config_base_data.copy()
fit_type_dir = os.path.join(output_directory, fit_type)
config_path = os.path.join(fit_type_dir, "config.json")
config["biophys"][0]["model_file"] = [ target_file, config_path]
if has_apic:
fit_style_file = os.path.join(FIT_BASE_DIR, 'fit_styles', '%s_fit_style.json' % (fit_type))
else:
fit_style_file = os.path.join(FIT_BASE_DIR, "fit_styles", "%s_noapic_fit_style.json" % (fit_type))
config["biophys"][0]["model_file"].append(fit_style_file)
config["manifest"].append({"type": "dir", "spec": fit_type_dir, "key": "FITDIR"})
ju.write(config_path, config)
for seed in SEEDS:
logfile = os.path.join(output_directory, fit_type, 's%d' % seed, 'stage_1.log')
jobs.append({
'config_path': os.path.abspath(config_path),
'fit_type': fit_type,
'log': os.path.abspath(logfile),
'seed': seed,
'num_processes': DEFAULT_NUM_PROCESSES
})
return jobs
#===============================================================================
# example 4
#===============================================================================
import allensdk.core.json_utilities as json_utilities
from allensdk.model.glif.glif_neuron import GlifNeuron
from allensdk.core.nwb_data_set import NwbDataSet
neuron_config = json_utilities.read('neuron_config.json')['566302806']
ephys_sweeps = json_utilities.read('ephys_sweeps.json')
ephys_file_name = 'stimulus.nwb'
# pull out the stimulus for the current-clamp first sweep
ephys_sweep = next( s for s in ephys_sweeps
if s['stimulus_units'] == 'Amps' )
ds = NwbDataSet(ephys_file_name)
data = ds.get_sweep(ephys_sweep['sweep_number'])
stimulus = data['stimulus']
# initialize the neuron
# important! update the neuron's dt for your stimulus
neuron = GlifNeuron.from_dict(neuron_config)
neuron.dt = 1.0 / data['sampling_rate']
# simulate the neuron
output = neuron.run(stimulus)
voltage = output['voltage']
threshold = output['threshold']
spike_times = output['interpolated_spike_times']
raw_ephys_file_name = '%d_raw_data.nwb' % dataset_id
if not os.path.isfile(raw_ephys_file_name):
print('Downloading data: %s'%raw_ephys_file_name)
ct.save_ephys_data(dataset_id, raw_ephys_file_name)
print('Saved: %s'%raw_ephys_file_name)
else:
print('File: %s already present...'%raw_ephys_file_name)
print('Loading data from: %s'%raw_ephys_file_name)
from allensdk.core.nwb_data_set import NwbDataSet
data_set = NwbDataSet(raw_ephys_file_name)
import matplotlib.pyplot as plt
import numpy as np
plt.figure()
sweep_numbers = sweep_numbers_for_data[dataset_id]
subset = {}
for sweep_number in sweep_numbers:
sweep_data = data_set.get_sweep(sweep_number)
# start/stop indices that exclude the experimental test pulse (if applicable)
index_range = sweep_data['index_range']
raw_ephys_file_name = '%d_raw_data.nwb' % dataset_id
info = {}
import h5py
import numpy as np
h5f = h5py.File(raw_ephys_file_name, "r")
metas = ['aibs_cre_line','aibs_dendrite_type','intracellular_ephys/Electrode 1/location']
for m in metas:
d = h5f.get('/general/%s'%m)
print("%s = \t%s"%(m,d.value))
info[m.split('/')[-1]]=str(d.value)
h5f.close()
from allensdk.core.nwb_data_set import NwbDataSet
data_set = NwbDataSet(raw_ephys_file_name)
sweep_numbers = data_set.get_experiment_sweep_numbers()
#sweep_numbers = [33,45]
sweep_numbers.sort()
print("All sweeps for %s: %s"%(dataset_id, sweep_numbers))
subthreshs = {}
spikings = {}
spike_count = {}
chosen = {}
stimuli = {}
import allensdk.core.json_utilities as json_utilities
from allensdk.model.glif.glif_neuron import GlifNeuron
from allensdk.core.nwb_data_set import NwbDataSet
neuron_config = json_utilities.read("neuron_config.json")
ephys_sweeps = json_utilities.read("ephys_sweeps.json")
ephys_file_name = "stimulus.nwb"
# pull out the stimulus for the first sweep
ephys_sweep = ephys_sweeps[0]
ds = NwbDataSet(ephys_file_name)
data = ds.get_sweep(ephys_sweep["sweep_number"])
stimulus = data["stimulus"]
# initialize the neuron
# important! update the neuron's dt for your stimulus
neuron = GlifNeuron.from_dict(neuron_config)
neuron.dt = 1.0 / data["sampling_rate"]
# simulate the neuron
output = neuron.run(stimulus)
voltage = output["voltage"]
threshold = output["threshold"]
spike_times = output["interpolated_spike_times"]
def save_cell_data_web(self, acceptable_stimtypes, non_standard_nwb=False,
ephys_dir='preprocessed', **kwargs):
bpopt_stimtype_map = utility.bpopt_stimtype_map
distinct_id_map = utility.aibs_stimname_map
nwb_file = NwbDataSet(self.nwb_path)
stim_map = defaultdict(list)
stim_sweep_map = {}
output_dir = os.path.join(os.getcwd(), ephys_dir)
utility.create_dirpath(output_dir)
sweep_numbers = kwargs.get('sweep_numbers') or nwb_file.get_sweep_numbers()
for sweep_number in sweep_numbers:
sweep_data = nwb_file.get_sweep_metadata(sweep_number)
stim_type = sweep_data['aibs_stimulus_name']
try:
stim_type = stim_type.decode('UTF-8')
except:
pass
if stim_type in acceptable_stimtypes:
sweep = nwb_file.get_sweep(sweep_number)
start_idx, stop_idx = sweep['index_range']
stimulus_trace = sweep['stimulus'][start_idx:stop_idx]
response_trace = sweep['response'][start_idx:stop_idx]
sampling_rate = sweep['sampling_rate']
time = np.arange(0, len(stimulus_trace)) / sampling_rate
trace_name = '%s_%d' % (
distinct_id_map[stim_type], sweep_number)
if non_standard_nwb:
calc_stimparams_func = self.calc_stimparams_nonstandard
else:
calc_stimparams_func = self.calc_stimparams
stim_start, stim_stop, stim_amp_start, stim_amp_end, \
tot_duration, hold_curr = calc_stimparams_func(
time, stimulus_trace, trace_name)
response_trace_short_filename = '%s.%s' % (trace_name, 'txt')
response_trace_filename = os.path.join(
output_dir, response_trace_short_filename)
time *= 1e3 # in ms
response_trace *= 1e3 # in mV
response_trace = utility.correct_junction_potential(response_trace,
self.junction_potential)
stimulus_trace *= 1e9
# downsampling
time, stimulus_trace, response_trace = utility.downsample_ephys_data(
time, stimulus_trace, response_trace)
if stim_type in utility.bpopt_current_play_stimtypes:
with open(response_trace_filename, 'wb') as response_trace_file:
np.savetxt(response_trace_file,
np.transpose([time, response_trace, stimulus_trace]))
else:
with open(response_trace_filename, 'wb') as response_trace_file:
np.savetxt(response_trace_file,
np.transpose([time, response_trace]))
holding_current = hold_curr # sweep['bias_current']
stim_map[distinct_id_map[stim_type]].append([
trace_name,
bpopt_stimtype_map[stim_type],
holding_current/1e12,
stim_amp_start / 1e12,
stim_amp_end/1e12,
stim_start * 1e3,
stim_stop * 1e3,
tot_duration * 1e3,
response_trace_short_filename])
stim_sweep_map[trace_name] = sweep_number
logger.debug('Writing stimmap.csv ...')
stim_reps_sweep_map, stimmap_filename = self.write_stimmap_csv(stim_map,
output_dir, stim_sweep_map)
self.write_provenance(
output_dir,
self.nwb_path,
stim_sweep_map,
stim_reps_sweep_map)
return output_dir, stimmap_filename
def extract_info_from_nwb_file(dataset_id, raw_ephys_file_name):
info = {}
import h5py
import numpy as np
h5f = h5py.File(raw_ephys_file_name, "r")
metas = ['aibs_cre_line','aibs_dendrite_type','intracellular_ephys/Electrode 1/location']
for m in metas:
d = h5f.get('/general/%s'%m)
print("%s = \t%s"%(m,d.value))
info[m.split('/')[-1]]=str(d.value)
h5f.close()
from allensdk.core.nwb_data_set import NwbDataSet
data_set = NwbDataSet(raw_ephys_file_name)
sweep_numbers = data_set.get_experiment_sweep_numbers()
if test:
sweep_numbers = [33,45]
sweep_numbers.sort()
info[DH.DATASET] = dataset_id
info[DH.COMMENT] = 'Data analysed on %s'%(time.ctime())
info[DH.PYELECTRO_VERSION] = pyel_ver
info[DH.ALLENSDK_VERSION] = allensdk_ver
info[DH.SWEEPS] = {}
for sweep_number in sweep_numbers:
sweep_data = data_set.get_sweep(sweep_number)
if data_set.get_sweep_metadata(sweep_number)['aibs_stimulus_name'] == "Long Square":
sweep_info = {}
sweep_info[DH.METADATA] = data_set.get_sweep_metadata(sweep_number)
info[DH.SWEEPS]['%i'%sweep_number] = sweep_info
sweep_info[DH.SWEEP] = sweep_number
# start/stop indices that exclude the experimental test pulse (if applicable)
index_range = sweep_data['index_range']
# stimulus is a numpy array in amps
stimulus = sweep_data['stimulus'][index_range[0]:index_range[-1]]
# response is a numpy array in volts
response = sweep_data['response'][index_range[0]:index_range[-1]]*1000
# sampling rate is in Hz
sampling_rate = sweep_data['sampling_rate']
# define some time points in seconds (i.e., convert to absolute time)
time_pts = np.arange(0,len(stimulus)/sampling_rate,1./sampling_rate)*1000
comment = 'Sweep: %i in %i; %sms -> %sms; %sA -> %sA; %smV -> %smV'%(sweep_number, dataset_id, time_pts[0], time_pts[-1], np.amin(stimulus), np.amax(stimulus), np.amin(response), np.amax(response))
print(comment)
sweep_info[DH.COMMENT] = comment
analysis = utils.simple_network_analysis({sweep_number:response},
time_pts,
extra_targets = ['%s:value_280'%sweep_number,
'%s:average_1000_1200'%sweep_number,
'%s:average_100_200'%sweep_number],
end_analysis=1500,
plot=plot,
show_plot_already=False,
verbose=True)
sweep_info[DH.ICLAMP_ANALYSIS] = analysis
analysis_file_name = '%s_analysis.json'%(dataset_id)
analysis_file = open(analysis_file_name, 'w')
pretty = pp.pformat(info)
pretty = pretty.replace('\'', '"')
pretty = pretty.replace('u"', '"')
analysis_file.write(pretty)
analysis_file.close()
print('Written info to %s'%analysis_file_name)
