def test_timeout(self):
start = time.time()
with self.assertRaisesRegex(
RuntimeError, "Child process .* timed out after 1 second"):
subprocessing.execute(["sleep", "10"], timeout=1)
elapsed = time.time() - start
assert elapsed < 1.5
def test_redirection(self):
result = subprocessing.execute(["echo", "test"], stdout=open(os.devnull, "w"))
with self.assertRaisesRegex(ValueError, "stdout was redirected to file, unable to access"):
result.stdout.strip()
assert not result.stderr
assert not result.return_code
assert result.get_command_string() == "echo test"
result = subprocessing.execute(["cat", "--bad-option"], stderr=open(os.devnull, "w"))
assert result.stdout.strip() == ""
with self.assertRaisesRegex(ValueError, "stderr was redirected to file, unable to access"):
assert result.stderr.startswith("cat: unrecognized")
assert result.return_code
def test_piping(self):
result = subprocessing.execute(["pwd"])
assert result.stdout.strip() == os.getcwd()
assert result.stderr.strip() == ""
assert not result.return_code and result.successful()
result = subprocessing.execute(["cat", "--bad-option"])
assert result.stdout.strip() == ""
assert result.stderr.startswith("cat: unrecognized")
assert result.return_code and not result.successful()
result = subprocessing.execute(["cat"], stdin="fish")
assert result.stdout.strip() == "fish"
assert not result.stderr
assert not result.return_code and result.successful()
def run_prodigal(record: Record, options: ConfigType) -> None:
""" Run progidal to annotate prokaryotic sequences
"""
if "basedir" in options.get('prodigal', ''):
basedir = options.prodigal.basedir
else:
basedir = ""
with TemporaryDirectory(change=True):
name = record.id.lstrip('-')
if not name:
name = "unknown"
fasta_file = '%s.fasta' % name
result_file = '%s.predict' % name
with open(fasta_file, 'w') as handle:
seqio.write([record.to_biopython()], handle, 'fasta')
# run prodigal
prodigal = [path.join(basedir, 'prodigal')]
prodigal.extend(['-i', fasta_file, '-f', 'sco', '-o', result_file])
if options.genefinding_tool == "prodigal-m" or len(record.seq) < 20000:
prodigal.extend(['-p', 'meta'])
err = execute(prodigal).stderr
if err.find('Error') > -1:
logging.error("Failed to run prodigal: %r", err)
raise RuntimeError("prodigal error: %s" % err)
found = 0
for line in open(result_file, 'r'):
# skip first line
if not line.startswith('>'):
continue
name, start_chunk, end_chunk, prodigal_strand = line[1:].rstrip(
).split("_")
try:
start = int(start_chunk)
end = int(end_chunk)
if prodigal_strand == "+":
strand = 1
else:
strand = -1
except ValueError:
logging.error('Malformatted prodigal output line %r',
line.rstrip())
continue
if start > end:
strand = -1
start, end = end, start
loc = FeatureLocation(start - 1, end, strand=strand)
translation = record.get_aa_translation_from_location(loc)
feature = CDSFeature(loc,
locus_tag='ctg%s_%s' %
(record.record_index, name),
translation=translation,
translation_table=record.transl_table)
record.add_cds_feature(feature)
found += 1
logging.debug("prodigal found %d CDS features", found)
def download_resfam(db_dir: str) -> None:
"""Download and sanitise the Resfam database."""
archive_filename = os.path.join(db_dir, "resfam", "Resfams.hmm.gz")
filename = os.path.splitext(archive_filename)[0]
url = RESFAM_URL
# checksum of existing not matched because it has a convert timestamp in it
# So check size and line count as an approximation
if present_and_size_matches(filename, RESFAM_SIZE) and \
present_and_line_count_matches(filename, RESFAM_LINES):
print("Resfams database present and checked")
return
print("Downloading Resfam database")
check_diskspace(url)
download_if_not_present(url, archive_filename, RESFAM_ARCHIVE_CHECKSUM)
filename = unzip_file(archive_filename, gzip, gzip.zlib.error) # type: ignore
delete_file(filename + ".gz")
# remove tabs
converted = execute(["hmmconvert", filename])
print("Ensuring all cutoffs are present")
# add TC to those entries missing them
# calculated as 10% less than the minimum scoring hit in their own group
missing_cutoffs = {
"RF0174": int(374 * 0.9),
"RF0172": int(85 * 0.9),
"RF0173": int(295 * 0.9),
"RF0168": int(691 * 0.9),
}
with open(filename, "w") as handle:
lines = list(converted.stdout)
i = 0
while i < len(lines):
# find an accession
while i < len(lines) and not lines[i].startswith("ACC"):
handle.write(lines[i])
i += 1
# end of file with no new accession
if i >= len(lines):
break
# write the accession line itself
handle.write(lines[i])
# add the cutoffs if missing
acc = lines[i].split()[1]
if acc not in missing_cutoffs:
continue
value = missing_cutoffs[acc]
# an accession of interest, so add cutoffs in the same place as others
while not lines[i].startswith("CKSUM"):
handle.write(lines[i])
i += 1
# write the CKSUM line
handle.write(lines[i])
# and finally add the cutoffs
for cutoff in ["GA", "TC", "NC"]:
handle.write("%s %d.00 %d.00\n" % (cutoff, value, value))
i += 1
ensure_database_pressed(filename)
def run_diamond(query: str, database: str, tempdir: str, options: ConfigType) -> str:
""" Runs diamond, comparing the given query to the given database
Arguments:
query: the path of query sequence file
target: the path of the database to compare to
tempdir: the path of a temporary directory for diamond to use
options: antismash Config
Returns:
the name of the output file created
"""
logging.debug("Running external command: diamond")
command = [
"diamond", "blastp",
"--db", database,
"--threads", str(options.cpus),
"--query", query,
"--compress", "0",
"--max-target-seqs", "10000",
"--evalue", "1e-05",
"--out", "input.out",
"--outfmt", "6", # 6 is blast tabular format, just as in blastp
"--tmpdir", tempdir
]
result = subprocessing.execute(command)
if not result.successful():
raise RuntimeError("diamond failed to run: %s -> %s" % (command, result.stderr[-100:]))
return "input.out"
def get_git_version() -> str:
"""Get the sha1 of the current git version"""
args = ['git', 'rev-parse', '--short', 'HEAD']
try:
return execute(args).stdout.strip()
except OSError:
pass
return ""
def get_git_version(fallback_filename: Optional[str] = GIT_VERSION_FALLBACK_FILENAME) -> str:
"""Get the sha1 of the current git version"""
git_version = ""
try:
version_cmd = execute(['git', 'rev-parse', '--short', 'HEAD'])
status_cmd = execute(['git', 'status', '--porcelain'])
if version_cmd.successful() and status_cmd.successful():
git_version = version_cmd.stdout.strip()
changes = status_cmd.stdout.splitlines()
if changes:
git_version += "(changed)"
except OSError:
pass
if git_version == "" and fallback_filename:
if locate_file(fallback_filename, silent=True):
with open(fallback_filename, 'rt') as handle:
git_version = handle.read().strip()
return git_version
def run_external(fasta_filename: str) -> str:
""" Runs glimmerhmm on the provided fasta file and returns the stdout output
from glimmerhmm.
"""
glimmerhmm = ['glimmerhmm', fasta_filename,
path.get_full_path(__file__, "data/train_crypto"), "-g"]
run_result = execute(glimmerhmm)
if run_result.stderr.find('ERROR') > -1:
logging.error("Failed to run GlimmerHMM: %r", run_result.stderr)
raise RuntimeError("Failed to run GlimmerHMM: %s" % run_result.stderr)
return run_result.stdout
def alignsmcogs(smcog: str, input_number: int) -> str:
""" Align to multiple sequence alignment, output as fasta file """
reference = path.get_full_path(__file__, "data", "%s_muscle.fasta" % str(smcog).lower())
output_filename = "muscle%d.fasta" % input_number
musclecommand = ["muscle", "-quiet", "-profile", "-in1", reference,
"-in2", "input" + str(input_number) + ".fasta",
"-out", output_filename]
result = subprocessing.execute(musclecommand)
if result.return_code:
raise RuntimeError("Muscle failed to run: %s, %s" % (musclecommand, result.stderr[-100:]))
return output_filename
def run_nrpspredictor(a_domains: List[AntismashDomain],
options: ConfigType) -> Dict[str, Prediction]:
""" Runs NRPSPredictor2 over the provided A domains.
Arguments:
a_domains: a list of AntismashDomains, one for each A domain
options: antismash options
Returns:
a dictionary mapping each domain name to a PredictorSVMResult
"""
# NRPSPredictor: extract AMP-binding + 120 residues N-terminal of this domain,
# extract 8 Angstrom residues and insert this into NRPSPredictor
nrps_predictor_dir = path.get_full_path(__file__, "external",
"NRPSPredictor2")
data_dir = os.path.join(nrps_predictor_dir, 'data')
lib_dir = os.path.join(nrps_predictor_dir, 'lib')
jar_file = os.path.join(nrps_predictor_dir, 'build', 'NRPSpredictor2.jar')
java_separator = ":"
if sys.platform == "win32":
java_separator = ";"
classpath = java_separator.join([
jar_file,
os.path.join(lib_dir, 'java-getopt-1.0.13.jar'),
os.path.join(lib_dir, 'Utilities.jar'),
os.path.join(lib_dir, 'libsvm.jar')
])
input_filename = "signatures.fa"
output_filename = "svm_output.txt"
bacterial = "1" if options.taxon == "bacteria" else '0'
signatures = [get_34_aa_signature(a_domain) for a_domain in a_domains]
with TemporaryDirectory(change=True):
# Get NRPSPredictor2 code predictions, output sig file for input for NRPSPredictor2 SVMs
with open(input_filename, "w") as handle:
for sig, domain in zip(signatures, a_domains):
handle.write("%s\t%s\n" % (sig, domain.get_name()))
# Run NRPSPredictor2 SVM
commands = [
'java',
'-Ddatadir=%s' % data_dir, '-cp', classpath,
'org.roettig.NRPSpredictor2.NRPSpredictor2', '-i', input_filename,
'-r', output_filename, '-s', '1', '-b', bacterial
]
result = subprocessing.execute(commands)
if not result.successful():
raise RuntimeError("NRPSPredictor2 failed: %s" % result.stderr)
with open(output_filename) as handle:
lines = handle.read().splitlines()[1:] # strip the header
return read_output(lines)
def hmmsearch(fasta_format: str, hmm: str) -> float:
""" Runs hmmsearch, only taking a single value from the output """
result = subprocessing.execute(["hmmsearch", "--noali", hmm, "-"], stdin=fasta_format)
if not result.successful():
logging.error("hmmsearch stderr: %s", result.stderr)
raise RuntimeError("hmmsearch exited non-zero")
if "[No targets detected" in result.stdout:
return 0.
text = result.stdout
start = text.find('Domain annotation for each sequence:')
end = text[start:].find('Internal pipeline statistics summary:')
lines = text[start:start + end].splitlines()
return float(lines[4].split()[2])
def make_blastdb(inputfile: str, db_prefix: str) -> subprocessing.RunResult:
""" Runs makeblastdb on the inputs to create a blast protein database
makeblastdb will create 3 files with the given prefix and the extensions:
.pin, .phr, .psq
Arguments:
inputfile: the input filename
db_prefix: the prefix to use for the created database
Returns:
a subprocessing.RunResult instance
"""
command = ["makeblastdb", "-in", inputfile, "-out", db_prefix, "-dbtype", "prot"]
result = subprocessing.execute(command)
if not result.successful():
raise RuntimeError("makeblastdb failed to run: %s -> %s" % (command, result.stderr[-100:]))
return result
def draw_tree(input_number: int, output_dir: str, tag: str) -> str:
""" Construct a PNG for display via fasttree
Returns:
the filename of the image generated
"""
matplotlib.use('Agg')
command = [
"fasttree", "-quiet", "-fastest", "-noml",
"trimmed_alignment%d.fasta" % input_number
]
run_result = subprocessing.execute(command)
if not run_result.successful():
raise RuntimeError("Fasttree failed to run successfully:",
run_result.stderr)
handle = StringIO(run_result.stdout)
tree_filename = os.path.join(output_dir, tag + '.png')
try:
tree = Phylo.read(handle, 'newick')
except NewickError:
logging.debug('Invalid newick tree for %r', tag)
return ''
# enforce a minimum distance between branches
max_size = max(tree.distance(node) for node in tree.get_terminals())
for clade in tree.get_nonterminals() + tree.get_terminals():
if not clade.branch_length:
clade.branch_length = max_size / 20
else:
clade.branch_length = abs(clade.branch_length) + max_size / 20
# change the colour of the query gene
label_colors = {tag: 'green'}
Phylo.draw(tree,
do_show=False,
label_colors=label_colors,
label_func=lambda node: str(node).replace("|", " "))
fig = matplotlib.pyplot.gcf()
fig.set_size_inches(20, (tree.count_terminals() / 3))
matplotlib.pyplot.axis('off')
fig.savefig(os.path.join(output_dir, tag + '.png'), bbox_inches='tight')
matplotlib.pyplot.close(fig)
return tree_filename
def run_blast(query: str, database: str) -> str:
""" Runs blastp, comparing the given query with the given database
An output file will be created, using the name of the query but with the
extension changed to .out
Arguments:
query: the path of query sequence file
target: the path of the database to compare to
Returns:
the name of the created output file
"""
out_file = query.rsplit(".", 1)[0] + ".out"
command = ["blastp", "-db", database, "-query", query, "-outfmt", "6",
"-max_target_seqs", "10000", "-evalue", "1e-05",
"-out", out_file]
res = subprocessing.execute(command)
if not res.successful():
raise RuntimeError("blastp run failed: %s..." % res.stderr[-200:])
return out_file
def compile_pfam(filename):
"""Compile a HMMer database with hmmpress."""
command = ['hmmpress', '-f', filename]
execute(command)
