def MapSynapsesAndSurfaces(input_meta_filename, output_meta_filename):
# read in the meta data files
input_data = ReadMetaData(input_meta_filename)
output_data = ReadMetaData(output_meta_filename)
input_synapse_directory = input_data.SynapseDirectory()
output_synapse_directory = output_data.SynapseDirectory()
# create the output synapse directory if it does not exist
if not os.path.exists(output_synapse_directory):
os.makedirs(output_synapse_directory, exist_ok=True)
# read in all of the input syanpses
input_synapses = {}
for iz in range(input_data.StartZ(), input_data.EndZ()):
for iy in range(input_data.StartY(), input_data.EndY()):
for ix in range(input_data.StartX(), input_data.EndX()):
input_synapse_filename = '{}/{:04d}z-{:04d}y-{:04d}x.pts'.format(input_synapse_directory, iz, iy, ix)
# ignore the local points
global_pts, _ = ReadPtsFile(input_data, input_synapse_filename)
for label in global_pts:
if not label in input_synapses:
input_synapses[label] = []
# add the array from this block to the input synapses
input_synapses[label] += global_pts[label]
# create an output dictionary of synapses per block
output_synapses_per_block = {}
# iterate over all output blocks
for iz in range(output_data.StartZ(), output_data.EndZ()):
for iy in range(output_data.StartY(), output_data.EndY()):
for ix in range(output_data.StartX(), output_data.EndX()):
# create empty synapses_per_block dictionaries whose keys will be labels
output_synapses_per_block[(iz, iy, ix)] = {}
# for every label, go through the discovered synapses from the input data
for label in input_synapses.keys():
input_synapses_per_label = input_synapses[label]
for input_global_index in input_synapses_per_label:
# the global iz, iy, ix coordinates remain the same across blocks
global_iz, global_iy, global_ix = input_data.GlobalIndexToIndices(input_global_index)
# get the new block from the global coordinates
output_block_iz = global_iz // output_data.BlockZLength()
output_block_iy = global_iy // output_data.BlockYLength()
output_block_ix = global_ix // output_data.BlockXLength()
if not label in output_synapses_per_block[(output_block_iz, output_block_iy, output_block_ix)]:
output_synapses_per_block[(output_block_iz, output_block_iy, output_block_ix)][label] = []
# get the new global index
output_global_index = output_data.GlobalIndicesToIndex(global_iz, global_iy, global_ix)
output_synapses_per_block[(output_block_iz, output_block_iy, output_block_ix)][label].append(output_global_index)
# write all of the synapse block files
output_synapses_directory = output_data.SynapseDirectory()
for iz in range(output_data.StartZ(), output_data.EndZ()):
for iy in range(output_data.StartY(), output_data.EndY()):
for ix in range(output_data.StartX(), output_data.EndX()):
output_synapse_filename = '{}/{:04d}z-{:04d}y-{:04d}x.pts'.format(output_synapse_directory, iz, iy, ix)
# write the pts file (use global indices)
WritePtsFile(output_data, output_synapse_filename, output_synapses_per_block[(iz, iy, ix)], input_local_indices = False)
# get the input/output directories for the surfaces
input_surfaces_directory = input_data.SurfacesDirectory()
output_surfaces_directory = output_data.SurfacesDirectory()
# create the output synapse directory if it does not exist
if not os.path.exists(output_surfaces_directory):
os.makedirs(output_surfaces_directory, exist_ok=True)
# iterate over all labels
for label in range(1, input_data.NLabels()):
start_time = time.time()
# skip over labels that do not exist
input_surface_filename = '{}/{:016d}.pts'.format(input_surfaces_directory, label)
if not os.path.exists(input_surface_filename): continue
# read in the input global points
input_global_points, _ = ReadPtsFile(input_data, input_surface_filename)
# create an empty dictionary for the output points
output_global_points = {}
output_global_points[label] = []
for input_global_index in input_global_points[label]:
# the global iz, iy, ix coordinates remain the same across blocks
global_iz, global_iy, global_ix = input_data.GlobalIndexToIndices(input_global_index)
# get the new global index
output_global_index = output_data.GlobalIndicesToIndex(global_iz, global_iy, global_ix)
output_global_points[label].append(output_global_index)
# write the new surface filename
output_surface_filename = '{}/{:016d}.pts'.format(output_surfaces_directory, label)
WritePtsFile(output_data, output_surface_filename, output_global_points, input_local_indices = False)
print ('Completed label {} in {:0.2f} seconds'.format(label, time.time() - start_time))
def EvaluateNeuralReconstructionIntegrity(meta_filename):
data = ReadMetaData(meta_filename)
synapses_per_label = {}
# read in all of the synapses from all of the blocks
synapse_directory = data.SynapseDirectory()
for iz in range(data.StartZ(), data.EndZ()):
for iy in range(data.StartY(), data.EndY()):
for ix in range(data.StartX(), data.EndX()):
synapses_filename = '{}/{:04d}z-{:04d}y-{:04d}x.pts'.format(synapse_directory, iz, iy, ix)
# ignore the local coordinates
block_synapses, _ = ReadPtsFile(data, synapses_filename)
# add all synapses for each label in this block to the global synapses
for label in block_synapses.keys():
if not label in synapses_per_label:
synapses_per_label[label] = []
synapses_per_label[label] += block_synapses[label]
# get the output filename
evaluation_directory = data.EvaluationDirectory()
if not os.path.exists(evaluation_directory):
os.makedirs(evaluation_directory, exist_ok=True)
output_filename = '{}/nri-results.txt'.format(evaluation_directory)
fd = open(output_filename, 'w')
# keep track of global statistics
total_true_positives = 0
total_false_positives = 0
total_false_negatives = 0
# for each label, find if synapses correspond to endpoints
for label in range(1, data.NLabels()):
# read the refined skeleton for this synapse
skeleton_directory = '{}/skeletons'.format(data.SkeletonOutputDirectory())
skeleton_filename = '{}/{:016d}.pts'.format(skeleton_directory, label)
# skip over labels not processed
if not os.path.exists(skeleton_filename): continue
# get the synapses only for this one label
synapses = synapses_per_label[label]
# ignore the local coordinates
skeletons, _ = ReadPtsFile(data, skeleton_filename)
skeleton = skeletons[label]
# read in the somata surfaces (points on the surface should not count as endpoints)
somata_directory = '{}/somata_surfaces'.format(data.TempDirectory())
somata_filename = '{}/{:016d}.pts'.format(somata_directory, label)
# path may not exist if soma not found
if os.path.exists(somata_filename):
somata_surfaces, _ = ReadPtsFile(data, somata_filename)
somata_surface = set(somata_surfaces[label])
else:
somata_surface = set()
# get the endpoints in this skeleton for this label
endpoints = FindEndpoints(data, skeleton, somata_surface)
true_positives, false_positives, false_negatives = CalculateNeuralReconstructionIntegrityScore(data, synapses, endpoints)
# if there are no true positives the NRI score is 0
if true_positives == 0:
nri_score = 0
else:
precision = true_positives / float(true_positives + false_positives)
recall = true_positives / float(true_positives + false_negatives)
nri_score = 2 * (precision * recall) / (precision + recall)
print ('Label: {}'.format(label))
print (' True Positives: {:10d}'.format(true_positives))
print (' False Positives: {:10d}'.format(false_positives))
print (' False Negatives: {:10d}'.format(false_negatives))
print (' NRI Score: {:0.4f}'.format(nri_score))
fd.write ('Label: {}\n'.format(label))
fd.write (' True Positives: {:10d}\n'.format(true_positives))
fd.write (' False Positives: {:10d}\n'.format(false_positives))
fd.write (' False Negatives: {:10d}\n'.format(false_negatives))
fd.write (' NRI Score: {:0.4f}\n'.format(nri_score))
# update the global stats
total_true_positives += true_positives
total_false_positives += false_positives
total_false_negatives += false_negatives
precision = total_true_positives / float(total_true_positives + total_false_positives)
recall = total_true_positives / float(total_true_positives + total_false_negatives)
nri_score = 2 * (precision * recall) / (precision + recall)
print ('Total Volume'.format(label))
print (' True Positives: {:10d}'.format(total_true_positives))
print (' False Positives: {:10d}'.format(total_false_positives))
print (' False Negatives: {:10d}'.format(total_false_negatives))
print (' NRI Score: {:0.4f}'.format(nri_score))
fd.write ('Total Volume\n'.format(label))
fd.write (' True Positives: {:10d}\n'.format(total_true_positives))
fd.write (' False Positives: {:10d}\n'.format(total_false_positives))
fd.write (' False Negatives: {:10d}\n'.format(total_false_negatives))
fd.write (' NRI Score: {:0.4f}\n'.format(nri_score))
def ConvertSynapsesAndProject(meta_filename, input_synapse_directory, xyz,
conversion_rate):
data = ReadMetaData(meta_filename)
resolution = data.Resolution()
# create an empty set of synapses
synapses = {}
# iterate over all labels
for label in range(1, data.NLabels()):
# read the surfaces for this label
surface_filename = '{}/{:016d}.pts'.format(data.SurfacesDirectory(),
label)
# some surfaces (i.e., labels) will not exist in the volume
if not os.path.exists(surface_filename): continue
# read in the surface points, ignore the local coordinates
surfaces, _ = ReadPtsFile(data, surface_filename)
surface = surfaces[label]
npts = len(surface)
surface_point_cloud = np.zeros((npts, 3), dtype=np.int32)
for index, iv in enumerate(surface):
iz, iy, ix = data.GlobalIndexToIndices(iv)
surface_point_cloud[index, :] = (iz * resolution[OR_Z],
iy * resolution[OR_Y],
ix * resolution[OR_X])
# create an empty array for the synapses
synapses[label] = []
projected = 0
missed = 0
# read in the original synapses
input_synapse_filename = '{}/syn_{:04}.txt'.format(
input_synapse_directory, label)
if os.path.exists(input_synapse_filename):
with open(input_synapse_filename, 'r') as fd:
for line in fd:
# separate the line into coordinates
coordinates = line.strip().split()
if xyz:
ix = round(int(coordinates[0]) / conversion_rate[OR_X])
iy = round(int(coordinates[1]) / conversion_rate[OR_Y])
iz = round(int(coordinates[2]) / conversion_rate[OR_Z])
else:
iz = round(int(coordinates[0]) / conversion_rate[OR_Z])
iy = round(int(coordinates[1]) / conversion_rate[OR_Y])
ix = round(int(coordinates[2]) / conversion_rate[OR_X])
# create a 2D vector for this point
vec = np.zeros((1, 3), dtype=np.int32)
vec[0, :] = (iz * resolution[OR_Z], iy * resolution[OR_Y],
ix * resolution[OR_X])
closest_point = surface[scipy.spatial.distance.cdist(
surface_point_cloud, vec).argmin()]
closest_iz, closest_iy, closest_ix = data.GlobalIndexToIndices(
closest_point)
deltaz = resolution[OR_Z] * (iz - closest_iz)
deltay = resolution[OR_Y] * (iy - closest_iy)
deltax = resolution[OR_X] * (ix - closest_ix)
distance = math.sqrt(deltaz * deltaz + deltay * deltay +
deltax * deltax)
# skip distances that are clearly off (over 200 nanometers)
max_deviation = 800
if distance < max_deviation:
# add to the list of valid synapses
synapses[label].append(closest_point)
projected += 1
else:
missed += 1
print('Synapses within {} nanometers from surface: {}'.format(
max_deviation, projected))
print('Synapses over {} nanometers from surface: {}'.format(
max_deviation, missed))
# divide all synapses into blocks
synapses_per_block = {}
# iterate over all blocks
for iz in range(data.StartZ(), data.EndZ()):
for iy in range(data.StartY(), data.EndY()):
for ix in range(data.StartX(), data.EndX()):
# create empty synapses_per_block dictionaries whose keys will be labels
synapses_per_block[(iz, iy, ix)] = {}
# for every label, iterate over the discovered synapses
for label in synapses.keys():
synapses_per_label = synapses[label]
# iterate over all of the projected synapses
for global_index in synapses_per_label:
global_iz, global_iy, global_ix = data.GlobalIndexToIndices(
global_index)
block_iz = global_iz // data.BlockZLength()
block_iy = global_iy // data.BlockYLength()
block_ix = global_ix // data.BlockXLength()
# create the array for this label per block if it does not exist
if not label in synapses_per_block[(block_iz, block_iy, block_ix)]:
synapses_per_block[(block_iz, block_iy, block_ix)][label] = []
synapses_per_block[(block_iz, block_iy,
block_ix)][label].append(global_index)
# write all of the synapse block files
synapse_directory = data.SynapseDirectory()
if not os.path.exists(synapse_directory):
os.makedirs(synapse_directory, exist_ok=True)
for iz in range(data.StartZ(), data.EndZ()):
for iy in range(data.StartY(), data.EndY()):
for ix in range(data.StartX(), data.EndX()):
synapse_filename = '{}/{:04d}z-{:04d}y-{:04d}x.pts'.format(
synapse_directory, iz, iy, ix)
# write the pts file (use global indices)
WritePtsFile(data,
synapse_filename,
synapses_per_block[(iz, iy, ix)],
input_local_indices=False)